CSPI05G26550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G26550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGABA transporter 1-like
LocationChr5: 25385529 .. 25390154 (+)
RNA-Seq ExpressionCSPI05G26550
SyntenyCSPI05G26550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACTAATTAATTATATAAGCTTTATCTCTTTGCTGTTCTCATGCATATATCTTCATTCCCCTTGAGATCAGAAAATTAAGTAGAAAGATATCAGTGAGAAATGGAAACGAAAGCAGCGATTATCTCTGGGGATACAATGGCGAAAGAGAATGGCAATGCTCACGTCCAGTTGACAGTAGATCAGCTGGATGCTGGAGCTCTATTTGTTCTTAAATCTCGAGGTTAGTGTTGGCCTTAGCTTCCTACTTCTTCCGTTTTCGTAAGTATACGTAAATCGTCCCCATGTTATTTTCAAATCATGTTAGGCATTGGCATGACACACAATAAACGTTGGTAGCTAGTATGAAAAAGTGAATGGTGTAACTTGTAGAAATGGAGAATTAAACTATCAATCTTTAAGATGAAAGATTATGTCAATTATGGTCGGAAGAACCTCTCGTCAGAAAAATAAAAGTGAAAATGAAAAAAAAAAATTAAAAACACAATAATTAACTTTATAAAATTTTCAAACCGGAGAAAAATCCACTACGTGAAGCTTTACTATCACACGTAATAATTCTCTCATGACTGCAATTATAATAGCACTTTCTTATAGTAAACTTTATGATTGCACTAATAATTATCTAAAACTTAAAATGTTTCTAACTGAGACGACTTAAATTAGAGGTATTTGCTTATCTTTTACGGTCCTAGGAGAAGAAAAACTCCATTTCTAACATTTTACTAAAAAAACTCAACAACTTACAAAACAAACAACTTGTTAATATTATATATTTGATTTCCCTTTTTCTATAGAAGTGGAGAATTCAAACTTTTGTATGAGTAGTTATTAATTGATAGTTGAATGAAATTGATGATAGGGTCATGGTGGCATTGTGGATATCACTTGACAACGTCAATAGTTGCTCCATCACTGTTAAGTCTTCCTTTTGCTTTCAGACTCCTTGGTTGGGTTGGTGGAATTATATGTCTTCTTTTCTGTGGTGTTGTCACTTTCTATGCCTACCATCTCTTATCTCTTGTTTTAGAGCACCATGCTCTACGTGGTTCTCGTTTACTTAGGTTTCGAGATATGGCTACAAATATTCTTGGTACGTTATCTTTTTCTTTTTATTAATTTTGTGCAATAAAAAATAATTAAAAAAATTATCAAAGAGTGATAGCTTAGTGTTAAATATTGATATGTACGGGAAGTTGAAGGTTCAAATTAGTACTTGTGAGATTTGGATAGAAAAAGAATATTTTTTTCAACGAAAAAATGTTAAATTACGAATATGGTATTTTTTTAAAAATCTTTTTTAGGTTAGCCTACGTCTAGATTTTCAGGTCAAATACATTTCCAAACTTTCAATTTTTTACTTTCTAAAAATATAACAAAAAAAAAAAAAAAACTTTATGTTAAGTTTTGAAAATTGTGAACAAAATTGTCAAACATTATAAAATTACAAAAGATGAAAATACAACCAAGGTCCAGAATAAATATTTTGAAATATATATATATATATATATATAGATGAAGTTATTTTACAAAACCTTTTTGCATTTTTTCTTAAATTTGCAAAACTTTACTGGAATTTAAAATTGAAAACAATAGTTTTCAAAAGAGAAAATTGTAAAAATAAAAAGAACTTTTATCAAAAAAAATATTTACCTTTATAAAAAAAAAATAGAAAATTACATCTCTAATTAATTTTAATTAGTGCATAATAATATACATAGAAAATCGTTAATTAGTGCAGGATAATATACATAAGAAATCGTAATAATATATAATTCTCAACTCTGTTCACTAAATAAATATTAATTAAATAAAAATTGAAAATTGAAAATATAACAAAAAAAATCTATTTAAAAAAGTATAGCAAAATTTTTTCGATTCTATATATTTTGTATATTTAACAAAAAAAAAAATATAGAAAAATCTATTATATTCTATGTCTAAAAAATTTTCATAAAGGAAAAGAAAAAAAAGAGAATATGGCTGAATTGAATGAAATAGGGATTCATTAGGCATAAGAGATCCTATAGTTGGATTAGGACTGCCTATTTGACTTATAGGATACAAAAGGAAAAGGAAAAGCAATGAATTATAATTGTGAGTTGTGAGAGTGGCAGGACCTAAATGGGCCATCTTCTACGTGGGCCCAATTCAATTTGGAGTATGCTACGGTTCGGTTGTTGCTGGAATTCTTATCGGTGGTCAAAACCTCAAGGTCACACCTCTTTCCTTCATTTTTCCATATTGCCAAATTTAGAATTATCAAATCAAAATATAGTCCACCTATAGAGACTTCCATGTGAAATTTAATTATAGATCGAGGCTACGTCTAAGTCTATCCACTCGTAAATGTTTGTTATAAATAGAGATGTCTATTTAACTAACAAGGTCCAAATGTCATTGAAAATATGTCCCGACGTAGATAATGGAGGAGTGAATGAGAATTATTTTAAAAAATATTTTTTGTTTAATTACGTTTAATGTTTTAAAAAATATGTTCTAGAATTCACCCTAAATTTTGTAATGAAAGAAGTTGTTTGTTTGAAGAATCGAAGTTGCTCACAATTATCATTACCATTATTCTTAACGTTATAGATATTGTTACGGTTGCAATATTAAAACTATGAATCAATCATATTTATTGTTAGTTTTTTTTCATTATTATCAGAGTCTAGCCGTAATTGTAATAGTAACAATAATAGGAAATAACTATAAATATGAAAAAATTATGTCTAGAGGTAACAGTAACAAATTCATCGTTCTAAGTTAACGATATAAAAATTTTATTACGATTTGTTAAATGTGGCTTAAGTATAACATATGAGACGATATTTAGAACAACATGTTTGACTTGCTCGACATGACTTTTCTAGTGTTTGAAACGTCATTATAAATATACATGATTACTTATGAGATCATTATTACAAATTTGAATGAAACTAAACCTCACCTTTGTGTTTTGTGTTTTTGGCAGTATATTTATGTGCTGTGCAATCCAGAGGGGGGAATGCAACTTTACCAATTCATAATTATATTTGGAACACTAATGTTGATTTTAGCACAAATTCCATCTTTCCATTCTCTAAGACATATAAACCTCATCTCTCTCACCCTATCCCTTGCTTATAGTGCTTGTGTAACTGCTGCTTCTTTAAAACTAGGTAAAAAAAACAACTTTGTATCAATATTTTCAAACTCATTTTTCTTTTGTAAAATTACCAATAAGGCCTTCCCATCACAGGGTTTTCCAAGAATGCCCCTCCAAGAGACTACTCTGTTAAAGGGTCGCCCGTCAGTCAGTTATTTAATGCCTTCAATGGCATTTCAGTAATTGCAACTGCCTATGCATGTGGAATGCTTCCCGAAATTCAGGTATAAATTATGGTTTAATATATATTTATACAAAATACGTCCAAATTTGACTTTCTCTCTTAACAAAATGTAGAAAGTTAAATTTTTTGAAAGTATGTTATAATAAGTGAAATGGGTATCTAATGACTTTTGACATATACGTTTAGAACTTTAAAATTTCATACTAGTCTTTACTTGTATACCTATGATAATATCTTTATTTAGTCGATATGGAACTTTGATCATATTTTTAACAATCATCTCTTCGAACAAAGGACTATTGAGTCTTTCTTCAAATAGTCATCTATATGTTTAACTCTTATCTAGGCTAACTTCTTCTCATTGTAGTATCGCATAGTACACCACCTAGCCAAGAGTTCTACCACTTTTGCTGTACGCCTTGGTCAACATATATTGCATAGATAATGTTTATAAGTTTCATTTTTAAAAATTAAATGATTATCCAACCGAGCATAAATGGTTAAGTTGATTGAAGGCTAAATTTTATCCTCCCTCTGAACTAAAATTGAATTGAATGATGAACACATGCAGGCAACTTTAGTAGCTCCACTAAAAGGAAAGATGTTCAAAGGGTTATGCCTTTGTTATACAGTGATAGCAACCACCTTTTTGAGCGTAGGAATTTCTGCATATTGGACTTTTGGAAATGAGGCAATGGGAACAGTTCTTACTAATTTCATGTCACAAAACTCTTTACCTTCTTGGCTTATCATCATCACCAATGCATTTTGCCTCACTCAAGTCTCTGCCGTTGCTGGCGTAAGACCCTATATCTTTTTTCCATTCTTTTCCTATTTGAATCTCAATATGGCATGATTTAGTAGAGTATCTATAATATGTATCTTAACTTTAATTTGATTGTTTATTTGCATAGACTTATTTACAACCCACGAACGAAGCATTTGAAAAGACATTCGCGGACCCAAACAAAGACCAATTTTCGATGCGAAACATTGTGCCAAGGTTAATATCACGTTCGTTATCGGTGGTGATAGCAACTATAGTTGGAGCCATGTTGCCTTTCTTTGGGGATTTAATGGCACTAATTGGAGCATTGGGTTTCATTCCTTTGGACTTCATTATGCCAATGGTTTTCTACAATGCCACTTTCAAGCCTTCTAAGCGTAGCTTTATTTATTGGATAAACACTTTGATTGTCGCAATTTCATCAGTATTGGCTATAATTGGTGGTGTTGCTTCCATTCGGCAAATCGTTTTGGATGCCAAAGAATATCGTTTGTTTGCCAATGTGTAA

mRNA sequence

GTACTAATTAATTATATAAGCTTTATCTCTTTGCTGTTCTCATGCATATATCTTCATTCCCCTTGAGATCAGAAAATTAAGTAGAAAGATATCAGTGAGAAATGGAAACGAAAGCAGCGATTATCTCTGGGGATACAATGGCGAAAGAGAATGGCAATGCTCACGTCCAGTTGACAGTAGATCAGCTGGATGCTGGAGCTCTATTTGTTCTTAAATCTCGAGGGTCATGGTGGCATTGTGGATATCACTTGACAACGTCAATAGTTGCTCCATCACTGTTAAGTCTTCCTTTTGCTTTCAGACTCCTTGGTTGGGTTGGTGGAATTATATGTCTTCTTTTCTGTGGTGTTGTCACTTTCTATGCCTACCATCTCTTATCTCTTGTTTTAGAGCACCATGCTCTACGTGGTTCTCGTTTACTTAGGTTTCGAGATATGGCTACAAATATTCTTGGACCTAAATGGGCCATCTTCTACGTGGGCCCAATTCAATTTGGAGTATGCTACGGTTCGGTTGTTGCTGGAATTCTTATCGGTGGTCAAAACCTCAAGTATATTTATGTGCTGTGCAATCCAGAGGGGGGAATGCAACTTTACCAATTCATAATTATATTTGGAACACTAATGTTGATTTTAGCACAAATTCCATCTTTCCATTCTCTAAGACATATAAACCTCATCTCTCTCACCCTATCCCTTGCTTATAGTGCTTGTGTAACTGCTGCTTCTTTAAAACTAGGGTTTTCCAAGAATGCCCCTCCAAGAGACTACTCTGTTAAAGGGTCGCCCGTCAGTCAGTTATTTAATGCCTTCAATGGCATTTCAGTAATTGCAACTGCCTATGCATGTGGAATGCTTCCCGAAATTCAGGCAACTTTAGTAGCTCCACTAAAAGGAAAGATGTTCAAAGGGTTATGCCTTTGTTATACAGTGATAGCAACCACCTTTTTGAGCGTAGGAATTTCTGCATATTGGACTTTTGGAAATGAGGCAATGGGAACAGTTCTTACTAATTTCATGTCACAAAACTCTTTACCTTCTTGGCTTATCATCATCACCAATGCATTTTGCCTCACTCAAGTCTCTGCCGTTGCTGGCACTTATTTACAACCCACGAACGAAGCATTTGAAAAGACATTCGCGGACCCAAACAAAGACCAATTTTCGATGCGAAACATTGTGCCAAGGTTAATATCACGTTCGTTATCGGTGGTGATAGCAACTATAGTTGGAGCCATGTTGCCTTTCTTTGGGGATTTAATGGCACTAATTGGAGCATTGGGTTTCATTCCTTTGGACTTCATTATGCCAATGGTTTTCTACAATGCCACTTTCAAGCCTTCTAAGCGTAGCTTTATTTATTGGATAAACACTTTGATTGTCGCAATTTCATCAGTATTGGCTATAATTGGTGGTGTTGCTTCCATTCGGCAAATCGTTTTGGATGCCAAAGAATATCGTTTGTTTGCCAATGTGTAA

Coding sequence (CDS)

ATGGAAACGAAAGCAGCGATTATCTCTGGGGATACAATGGCGAAAGAGAATGGCAATGCTCACGTCCAGTTGACAGTAGATCAGCTGGATGCTGGAGCTCTATTTGTTCTTAAATCTCGAGGGTCATGGTGGCATTGTGGATATCACTTGACAACGTCAATAGTTGCTCCATCACTGTTAAGTCTTCCTTTTGCTTTCAGACTCCTTGGTTGGGTTGGTGGAATTATATGTCTTCTTTTCTGTGGTGTTGTCACTTTCTATGCCTACCATCTCTTATCTCTTGTTTTAGAGCACCATGCTCTACGTGGTTCTCGTTTACTTAGGTTTCGAGATATGGCTACAAATATTCTTGGACCTAAATGGGCCATCTTCTACGTGGGCCCAATTCAATTTGGAGTATGCTACGGTTCGGTTGTTGCTGGAATTCTTATCGGTGGTCAAAACCTCAAGTATATTTATGTGCTGTGCAATCCAGAGGGGGGAATGCAACTTTACCAATTCATAATTATATTTGGAACACTAATGTTGATTTTAGCACAAATTCCATCTTTCCATTCTCTAAGACATATAAACCTCATCTCTCTCACCCTATCCCTTGCTTATAGTGCTTGTGTAACTGCTGCTTCTTTAAAACTAGGGTTTTCCAAGAATGCCCCTCCAAGAGACTACTCTGTTAAAGGGTCGCCCGTCAGTCAGTTATTTAATGCCTTCAATGGCATTTCAGTAATTGCAACTGCCTATGCATGTGGAATGCTTCCCGAAATTCAGGCAACTTTAGTAGCTCCACTAAAAGGAAAGATGTTCAAAGGGTTATGCCTTTGTTATACAGTGATAGCAACCACCTTTTTGAGCGTAGGAATTTCTGCATATTGGACTTTTGGAAATGAGGCAATGGGAACAGTTCTTACTAATTTCATGTCACAAAACTCTTTACCTTCTTGGCTTATCATCATCACCAATGCATTTTGCCTCACTCAAGTCTCTGCCGTTGCTGGCACTTATTTACAACCCACGAACGAAGCATTTGAAAAGACATTCGCGGACCCAAACAAAGACCAATTTTCGATGCGAAACATTGTGCCAAGGTTAATATCACGTTCGTTATCGGTGGTGATAGCAACTATAGTTGGAGCCATGTTGCCTTTCTTTGGGGATTTAATGGCACTAATTGGAGCATTGGGTTTCATTCCTTTGGACTTCATTATGCCAATGGTTTTCTACAATGCCACTTTCAAGCCTTCTAAGCGTAGCTTTATTTATTGGATAAACACTTTGATTGTCGCAATTTCATCAGTATTGGCTATAATTGGTGGTGTTGCTTCCATTCGGCAAATCGTTTTGGATGCCAAAGAATATCGTTTGTTTGCCAATGTGTAA

Protein sequence

METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV*
Homology
BLAST of CSPI05G26550 vs. ExPASy Swiss-Prot
Match: F4HW02 (GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.2e-148
Identity = 252/434 (58.06%), Postives = 333/434 (76.73%), Query Frame = 0

Query: 29  LDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYA 88
           +DAG+LFVLKS+G+WWHCG+HLTTSIVAP+LLSLP+AF+ LGW  GI CL+    VTFY+
Sbjct: 18  VDAGSLFVLKSKGTWWHCGFHLTTSIVAPALLSLPYAFKFLGWAAGISCLVGGAAVTFYS 77

Query: 89  YHLLSLVLEHHALRGSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQN 148
           Y LLSL LEHHA  G+R LRFRDMA +IL PKW  +YVGPIQ  VCYG V+A  L+GGQ 
Sbjct: 78  YTLLSLTLEHHASLGNRYLRFRDMAHHILSPKWGRYYVGPIQMAVCYGVVIANALLGGQC 137

Query: 149 LKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAA 208
           LK +Y++  P G M+L++F+IIFG L+L+LAQ PSFHSLR+IN +SL L L YSA   AA
Sbjct: 138 LKAMYLVVQPNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAA 197

Query: 209 SLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMF 268
           S+ +G   NAP +DY++ G P +++F  FN +++IAT Y  G++PEIQAT+ AP+KGKM 
Sbjct: 198 SIYIGKEPNAPEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMM 257

Query: 269 KGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNS----LPSWLIIITNAFCL 328
           KGLC+CY V+  TF +V I+ YW FG +A G + TNF++  +    +P+W I + N F +
Sbjct: 258 KGLCMCYLVVIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTV 317

Query: 329 TQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFG 388
            Q+SAVA  YLQP N+  E   +DP K +FS+RN++PRL+ RSL VV+ATIV AMLPFFG
Sbjct: 318 LQLSAVAVVYLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFG 377

Query: 389 DLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQ 448
           D+ +L+GA GFIPLDF++P+VF+N TFKPSK+SFI+WINT+I  + S L +I  VA++RQ
Sbjct: 378 DVNSLLGAFGFIPLDFVLPVVFFNFTFKPSKKSFIFWINTVIAVVFSCLGVIAMVAAVRQ 437

Query: 449 IVLDAKEYRLFANV 459
           I++DA  Y+LFA+V
Sbjct: 438 IIIDANTYKLFADV 451

BLAST of CSPI05G26550 vs. ExPASy Swiss-Prot
Match: Q8L4X4 (Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.8e-111
Identity = 210/431 (48.72%), Postives = 287/431 (66.59%), Query Frame = 0

Query: 30  DAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAY 89
           DAGALFVL+S+G WWH G+HLTT+IV P++L+LP+AFR LGW  G +CL   G+VTFYAY
Sbjct: 18  DAGALFVLQSKGEWWHAGFHLTTAIVGPTILTLPYAFRGLGWWLGFVCLTTMGLVTFYAY 77

Query: 90  HLLSLVLEHHALRGSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNL 149
           +L+S VL+H    G R +RFR++A ++LG     + V  IQ  +  G  +  IL+ GQ L
Sbjct: 78  YLMSKVLDHCEKSGRRHIRFRELAADVLGSGLMFYVVIFIQTAINTGIGIGAILLAGQCL 137

Query: 150 KYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAAS 209
             +Y    P+G ++LY+FI +   +M++L+Q+PSFHSLRHIN  SL LSL Y+  V  A 
Sbjct: 138 DIMYSSLFPQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINCASLLLSLGYTFLVVGAC 197

Query: 210 LKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMFK 269
           + LG SKNAP R+YS++ S   ++F+AF  IS+IA  +  G+LPEIQATL  P  GKM K
Sbjct: 198 INLGLSKNAPKREYSLEHSDSGKVFSAFTSISIIAAIFGNGILPEIQATLAPPATGKMLK 257

Query: 270 GLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNS---LPSWLIIITNAFCLTQ 329
           GL LCY+VI  TF S  IS YW FGN +   +L N M        P  +I +   F L Q
Sbjct: 258 GLLLCYSVIFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPTLAPIVVIGLAVIFVLLQ 317

Query: 330 VSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDL 389
           + A+   Y Q   E  EK  AD  K  FS RN+VPRLI R+L +     + AMLPFFGD+
Sbjct: 318 LFAIGLVYSQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLYMAFCGFMAAMLPFFGDI 377

Query: 390 MALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIV 449
            A++GA GFIPLDF++PM+ YN T+KP++RSF YWIN  I+ + +   ++G  +SIR++V
Sbjct: 378 NAVVGAFGFIPLDFVLPMLLYNMTYKPTRRSFTYWINMTIMVVFTCAGLMGAFSSIRKLV 437

Query: 450 LDAKEYRLFAN 458
           LDA +++LF++
Sbjct: 438 LDANKFKLFSS 448

BLAST of CSPI05G26550 vs. ExPASy Swiss-Prot
Match: Q9C733 (Lysine histidine transporter-like 1 OS=Arabidopsis thaliana OX=3702 GN=At1g48640 PE=3 SV=2)

HSP 1 Score: 187.6 bits (475), Expect = 3.2e-46
Identity = 124/421 (29.45%), Postives = 211/421 (50.12%), Query Frame = 0

Query: 43  WWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALR 102
           WW+  +H  T++V   +L LPF    LGW  GI  L+   ++T Y   L  +V  H  + 
Sbjct: 45  WWYSTFHNVTAMVGAGVLGLPFFMAQLGWGPGIAVLILSWIITLYT--LWQMVEMHEMVP 104

Query: 103 GSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIY-VLCNPEGG 162
           G R  R+ ++     G +  ++ + P Q  V  G  +  ++ GGQ+LK  + + C     
Sbjct: 105 GKRFDRYHELGQFAFGERLGLYIIVPQQIIVEVGVCIVYMVTGGQSLKKFHEIACQDCSP 164

Query: 163 MQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPR 222
           ++L  FI+IF +   +L+ +P+F+S+  ++L++  +SL+YS     A+   G  ++    
Sbjct: 165 IRLSFFIMIFASSHFVLSHLPNFNSISGVSLVAAVMSLSYSTIAWTATAAKGVQEDV--- 224

Query: 223 DYSVK-GSPVSQLFNAFNGISVIATAYA-CGMLPEIQATLVA----PLKGKMFKGLCLCY 282
            Y  K G+  S + + F G+  IA AYA   ++ EIQAT+ +    P KG M++G+ + Y
Sbjct: 225 QYGYKSGTTASTVLSFFTGLGGIAFAYAGHNVVLEIQATIPSTPSNPSKGPMWRGVVVAY 284

Query: 283 TVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYL 342
            V+A  +  V +  Y  FGN     VL N +     P W I   N F +  V      + 
Sbjct: 285 VVVALCYFPVALVGYGVFGN----AVLDNVLMSLETPVWAIATANLFVVMHVIGSYQIFA 344

Query: 343 QPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALGF 402
            P  +  E TF    K  F    ++ R I R++ V +   +G M+PFFG L+A  G   F
Sbjct: 345 MPVFDMVE-TFL-VKKLNFKPSTVL-RFIVRNVYVALTMFIGIMIPFFGGLLAFFGGFAF 404

Query: 403 IPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLF 457
            P  + +P + +   +KP + S  +W N + + +  VL I+  +  +RQI++ +K+Y  F
Sbjct: 405 APTSYFLPCIMWLLIYKPKRFSLSWWTNWVCIVLGVVLMILSSIGGLRQIIIQSKDYSFF 453

BLAST of CSPI05G26550 vs. ExPASy Swiss-Prot
Match: Q9C9J0 (Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680 PE=2 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 2.3e-44
Identity = 131/422 (31.04%), Postives = 211/422 (50.00%), Query Frame = 0

Query: 43  WWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALR 102
           W++  +H  T++V   +L LPFA   LGW  G++ ++    +TFY+  L  +V  H A+ 
Sbjct: 39  WYYSAFHNVTAMVGAGVLGLPFAMSQLGWGPGLVAIIMSWAITFYS--LWQMVQLHEAVP 98

Query: 103 GSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNP--EG 162
           G RL R+ ++     GPK   + V P Q  V   S +   + GG++LK    L  P  E 
Sbjct: 99  GKRLDRYPELGQEAFGPKLGYWIVMPQQLLVQIASDIVYNVTGGKSLKKFVELLFPNLEH 158

Query: 163 GMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPP 222
             Q Y +I+ F  L L+L+Q P F+S++ ++L++  +S  YS   + AS+  G      P
Sbjct: 159 IRQTY-YILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGTEHR--P 218

Query: 223 RDYSVKGSPV-SQLFNAFNGISVIATAYA-CGMLPEIQATLVA----PLKGKMFKGLCLC 282
             Y V+G  V S +F+AFNGI  IA A+A   ++ EIQAT+ +    P K  M+KG+ + 
Sbjct: 219 STYGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVVVA 278

Query: 283 YTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTY 342
           Y ++   +L V IS YW FG      VL +       P+WLI   N         V G+Y
Sbjct: 279 YIIVIICYLFVAISGYWAFGAHVEDDVLISL----ERPAWLIAAANFMVFIH---VIGSY 338

Query: 343 LQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALG 402
                  F+   +   K      +   RL++RS  V +  +V   +PFFG L+   G L 
Sbjct: 339 QVFAMIVFDTIESYLVKTLKFTPSTTLRLVARSTYVALICLVAVCIPFFGGLLGFFGGLV 398

Query: 403 FIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRL 457
           F    + +P + +    +P + S  +W + + +     +AI+  +  +R I+L A+ Y+L
Sbjct: 399 FSSTSYFLPCIIWLIMKRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILSARTYKL 448

BLAST of CSPI05G26550 vs. ExPASy Swiss-Prot
Match: Q9SJP9 (Proline transporter 3 OS=Arabidopsis thaliana OX=3702 GN=PROT3 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.6e-43
Identity = 130/430 (30.23%), Postives = 212/430 (49.30%), Query Frame = 0

Query: 39  SRGSWWHCGYHLTTSIVAPSLLSLPFAFRL-LGWVGGIICLLFCGVVTFYAYHLLSLVLE 98
           S  SW+   + LTTSI +  +L       + LGW+GG++ L+    ++ YA    +LV +
Sbjct: 26  SSDSWFQAAFVLTTSINSAYVLGYSGTVMVPLGWIGGVVGLILATAISLYAN---TLVAK 85

Query: 99  HHALRGSRLLRFRDMATNILGPK-----WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYI 158
            H   G R +R+RD+A  I G K     W + YV        +      I++ G  LK +
Sbjct: 86  LHEFGGKRHIRYRDLAGFIYGRKAYCLTWVLQYVNLFMINCGF------IILAGSALKAV 145

Query: 159 YVLCNPEGGMQLYQFIIIFGTLMLILA-QIPSFHSLRHINLISLTLSLAYSACVTAASLK 218
           YVL   +  M+L  FI I G +  + A  IP   +L     +S  LSL Y       S+K
Sbjct: 146 YVLFRDDHAMKLPHFIAIAGLICAVFAIGIPHLSALGIWLAVSTILSLIYIVVAIVLSVK 205

Query: 219 LGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMFKGL 278
            G    AP RDY ++GSP+S+LF      + +   +  GMLPEIQAT+  P+   M K L
Sbjct: 206 DGV--KAPSRDYEIQGSPLSKLFTITGAAATLVFVFNTGMLPEIQATVKQPVVKNMMKAL 265

Query: 279 CLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVA 338
              +TV      +V    YW +G+     +L N       P W+  + N   + Q     
Sbjct: 266 YFQFTVGVLPMFAVVFIGYWAYGSSTSPYLLNNVNG----PLWVKALANISAILQSVISL 325

Query: 339 GTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIG 398
             +  PT E  +  F     +  +++N++ R+++R   + ++T++ A+LPF GD M+L G
Sbjct: 326 HIFASPTYEYMDTKFGIKG-NPLALKNLLFRIMARGGYIAVSTLLSALLPFLGDFMSLTG 385

Query: 399 ALGFIPLDFIMP-MVFYNATFKP--SKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLD 458
           A+   PL FI+   ++Y A      + +   +W+N +  ++ SV A    +A++R I LD
Sbjct: 386 AVSTFPLTFILANHMYYKAKNNKLNTLQKLCHWLNVVFFSLMSVAA---AIAALRLIALD 436

BLAST of CSPI05G26550 vs. ExPASy TrEMBL
Match: A0A0A0KWN7 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G615820 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 6.7e-257
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60
           METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL
Sbjct: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60

Query: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120
           SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK
Sbjct: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120

Query: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180
           WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ
Sbjct: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180

Query: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240
           IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI
Sbjct: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240

Query: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300
           SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT
Sbjct: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300

Query: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360
           VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV
Sbjct: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360

Query: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420
           PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY
Sbjct: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420

Query: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. ExPASy TrEMBL
Match: A0A5D3CBK1 (GABA transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002160 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 450/458 (98.25%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60
           METKA IISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL
Sbjct: 1   METKAPIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60

Query: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120
           SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK
Sbjct: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120

Query: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180
           WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFG LMLILAQ
Sbjct: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGGLMLILAQ 180

Query: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240
           IPSFHSLRHINLISLTLSLAYSACVTAASLKLG SKNAPPRDYSVKGSPV QLFNAFNGI
Sbjct: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGLSKNAPPRDYSVKGSPVGQLFNAFNGI 240

Query: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300
           SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCY VIATTFLSVGIS+YWTFGNEAMGT
Sbjct: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYAVIATTFLSVGISSYWTFGNEAMGT 300

Query: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360
           VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNK+QFSMRNIV
Sbjct: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKNQFSMRNIV 360

Query: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420
           PRLISRSLSV+IATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY
Sbjct: 361 PRLISRSLSVIIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420

Query: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. ExPASy TrEMBL
Match: A0A1S3BGK0 (GABA transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103489410 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 450/458 (98.25%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60
           METKA IISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL
Sbjct: 1   METKAPIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60

Query: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120
           SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK
Sbjct: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120

Query: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180
           WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFG LMLILAQ
Sbjct: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGGLMLILAQ 180

Query: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240
           IPSFHSLRHINLISLTLSLAYSACVTAASLKLG SKNAPPRDYSVKGSPV QLFNAFNGI
Sbjct: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGLSKNAPPRDYSVKGSPVGQLFNAFNGI 240

Query: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300
           SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCY VIATTFLSVGIS+YWTFGNEAMGT
Sbjct: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYAVIATTFLSVGISSYWTFGNEAMGT 300

Query: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360
           VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNK+QFSMRNIV
Sbjct: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKNQFSMRNIV 360

Query: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420
           PRLISRSLSV+IATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY
Sbjct: 361 PRLISRSLSVIIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420

Query: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. ExPASy TrEMBL
Match: A0A5D3CDF2 (GABA transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002170 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.4e-222
Identity = 403/456 (88.38%), Postives = 421/456 (92.32%), Query Frame = 0

Query: 6   AIISGDTMA---KENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSL 65
           A ISG   A   KE G A VQ T  +LDAGALFVLKSRGSWWHCGYHLTTSIVAP+LLSL
Sbjct: 5   APISGADQAAADKEKGGALVQPTA-ELDAGALFVLKSRGSWWHCGYHLTTSIVAPALLSL 64

Query: 66  PFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPKWA 125
           PFAFRLLGWVGG ICLL  GVVTFYAY LLSLVLEHHA++GSRLLRFRDMAT ILGPKWA
Sbjct: 65  PFAFRLLGWVGGSICLLLGGVVTFYAYLLLSLVLEHHAMQGSRLLRFRDMATYILGPKWA 124

Query: 126 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIP 185
           IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEG MQLYQFIIIFGTLMLILAQIP
Sbjct: 125 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGEMQLYQFIIIFGTLMLILAQIP 184

Query: 186 SFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISV 245
           SFHSLRHINL+SLTLSLAYSA  TAASL LG+SKNAPPRDYS++GSPVSQLFNAFNGISV
Sbjct: 185 SFHSLRHINLVSLTLSLAYSASATAASLILGYSKNAPPRDYSLQGSPVSQLFNAFNGISV 244

Query: 246 IATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVL 305
           IAT YACGMLPEIQATLVAP+KGKMFKGLCLCYTVIA TFLSVGIS YWTFGNEAMGTVL
Sbjct: 245 IATTYACGMLPEIQATLVAPVKGKMFKGLCLCYTVIAVTFLSVGISGYWTFGNEAMGTVL 304

Query: 306 TNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPR 365
           +NFM+ NSLPSWL+IITN FC  QVSAVAGTYLQPTNE FEK FADPNK+QFSMRNIVPR
Sbjct: 305 SNFMAHNSLPSWLLIITNIFCFLQVSAVAGTYLQPTNEVFEKIFADPNKNQFSMRNIVPR 364

Query: 366 LISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWI 425
           LISRSLSVV+A I+GAMLPFFGDLMALIGA GFIPLDFIMPM+FYNATFKPSK S IYW+
Sbjct: 365 LISRSLSVVVAIIIGAMLPFFGDLMALIGAFGFIPLDFIMPMIFYNATFKPSKHSLIYWV 424

Query: 426 NTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           NTLIVA+SSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 425 NTLIVAVSSVLAIIGGVASIRQIVLDAKEYRLFANV 459

BLAST of CSPI05G26550 vs. ExPASy TrEMBL
Match: A0A1S3BGQ7 (GABA transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103489411 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.4e-222
Identity = 403/456 (88.38%), Postives = 421/456 (92.32%), Query Frame = 0

Query: 6   AIISGDTMA---KENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSL 65
           A ISG   A   KE G A VQ T  +LDAGALFVLKSRGSWWHCGYHLTTSIVAP+LLSL
Sbjct: 5   APISGADQAAADKEKGGALVQPTA-ELDAGALFVLKSRGSWWHCGYHLTTSIVAPALLSL 64

Query: 66  PFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPKWA 125
           PFAFRLLGWVGG ICLL  GVVTFYAY LLSLVLEHHA++GSRLLRFRDMAT ILGPKWA
Sbjct: 65  PFAFRLLGWVGGSICLLLGGVVTFYAYLLLSLVLEHHAMQGSRLLRFRDMATYILGPKWA 124

Query: 126 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIP 185
           IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEG MQLYQFIIIFGTLMLILAQIP
Sbjct: 125 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGEMQLYQFIIIFGTLMLILAQIP 184

Query: 186 SFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISV 245
           SFHSLRHINL+SLTLSLAYSA  TAASL LG+SKNAPPRDYS++GSPVSQLFNAFNGISV
Sbjct: 185 SFHSLRHINLVSLTLSLAYSASATAASLILGYSKNAPPRDYSLQGSPVSQLFNAFNGISV 244

Query: 246 IATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVL 305
           IAT YACGMLPEIQATLVAP+KGKMFKGLCLCYTVIA TFLSVGIS YWTFGNEAMGTVL
Sbjct: 245 IATTYACGMLPEIQATLVAPVKGKMFKGLCLCYTVIAVTFLSVGISGYWTFGNEAMGTVL 304

Query: 306 TNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPR 365
           +NFM+ NSLPSWL+IITN FC  QVSAVAGTYLQPTNE FEK FADPNK+QFSMRNIVPR
Sbjct: 305 SNFMAHNSLPSWLLIITNIFCFLQVSAVAGTYLQPTNEVFEKIFADPNKNQFSMRNIVPR 364

Query: 366 LISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWI 425
           LISRSLSVV+A I+GAMLPFFGDLMALIGA GFIPLDFIMPM+FYNATFKPSK S IYW+
Sbjct: 365 LISRSLSVVVAIIIGAMLPFFGDLMALIGAFGFIPLDFIMPMIFYNATFKPSKHSLIYWV 424

Query: 426 NTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           NTLIVA+SSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 425 NTLIVAVSSVLAIIGGVASIRQIVLDAKEYRLFANV 459

BLAST of CSPI05G26550 vs. NCBI nr
Match: XP_004142456.1 (GABA transporter 1 [Cucumis sativus] >KGN52211.1 hypothetical protein Csa_008337 [Cucumis sativus])

HSP 1 Score: 896.0 bits (2314), Expect = 1.4e-256
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60
           METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL
Sbjct: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60

Query: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120
           SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK
Sbjct: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120

Query: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180
           WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ
Sbjct: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180

Query: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240
           IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI
Sbjct: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240

Query: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300
           SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT
Sbjct: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300

Query: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360
           VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV
Sbjct: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360

Query: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420
           PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY
Sbjct: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420

Query: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. NCBI nr
Match: XP_008446787.1 (PREDICTED: GABA transporter 1-like [Cucumis melo] >KAA0034672.1 GABA transporter 1-like [Cucumis melo var. makuwa] >TYK09223.1 GABA transporter 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 450/458 (98.25%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   METKAAIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60
           METKA IISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL
Sbjct: 1   METKAPIISGDTMAKENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLL 60

Query: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120
           SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK
Sbjct: 61  SLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPK 120

Query: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQ 180
           WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFG LMLILAQ
Sbjct: 121 WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGGLMLILAQ 180

Query: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGI 240
           IPSFHSLRHINLISLTLSLAYSACVTAASLKLG SKNAPPRDYSVKGSPV QLFNAFNGI
Sbjct: 181 IPSFHSLRHINLISLTLSLAYSACVTAASLKLGLSKNAPPRDYSVKGSPVGQLFNAFNGI 240

Query: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGT 300
           SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCY VIATTFLSVGIS+YWTFGNEAMGT
Sbjct: 241 SVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYAVIATTFLSVGISSYWTFGNEAMGT 300

Query: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIV 360
           VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNK+QFSMRNIV
Sbjct: 301 VLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKNQFSMRNIV 360

Query: 361 PRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420
           PRLISRSLSV+IATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY
Sbjct: 361 PRLISRSLSVIIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIY 420

Query: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 WINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. NCBI nr
Match: XP_038891456.1 (GABA transporter 1-like [Benincasa hispida])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 418/460 (90.87%), Postives = 436/460 (94.78%), Query Frame = 0

Query: 1   METKAAIISGDTMA--KENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPS 60
           METKA  ISGDTMA  KE+ NAHV LTV +LDAGALFVLKSRGSWWHCGYHLTTSIVAPS
Sbjct: 1   METKAP-ISGDTMAKEKESSNAHVHLTV-ELDAGALFVLKSRGSWWHCGYHLTTSIVAPS 60

Query: 61  LLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILG 120
           LLSLPFAFRLLGWVGGIICLLF G VTFYAYHLLSLVLEHHA+RGSRLLRFRDMATNILG
Sbjct: 61  LLSLPFAFRLLGWVGGIICLLFGGFVTFYAYHLLSLVLEHHAMRGSRLLRFRDMATNILG 120

Query: 121 PKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLIL 180
           PKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEG MQLY+FIIIFG LMLIL
Sbjct: 121 PKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGRMQLYEFIIIFGALMLIL 180

Query: 181 AQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFN 240
           AQIPSFHS+RHINLISLTLSLAYSACVTAASLKLG+SKNAPPRDYSV+GSPVSQLFNAFN
Sbjct: 181 AQIPSFHSMRHINLISLTLSLAYSACVTAASLKLGYSKNAPPRDYSVEGSPVSQLFNAFN 240

Query: 241 GISVIATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAM 300
           GISVIAT YACGMLPEIQATLVAP+KGKMFKGLCLCY VIA TFLSVGIS YWTFG EAM
Sbjct: 241 GISVIATTYACGMLPEIQATLVAPVKGKMFKGLCLCYAVIAATFLSVGISGYWTFGKEAM 300

Query: 301 GTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRN 360
           GTVL+NFMS N LPSWL+IITNAFCL QVSAVAGTYLQPTNE FEK FADPNK+QFSMRN
Sbjct: 301 GTVLSNFMSNNLLPSWLLIITNAFCLLQVSAVAGTYLQPTNEVFEKKFADPNKNQFSMRN 360

Query: 361 IVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSF 420
           IVPR+I+RSLSVVIATI+GAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKR +
Sbjct: 361 IVPRIIARSLSVVIATIIGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRGY 420

Query: 421 IYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           +YW+NTLIV +SSVLA IGGVASIRQIVLDAKEYRLFANV
Sbjct: 421 LYWLNTLIVVVSSVLAFIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. NCBI nr
Match: XP_038891453.1 (GABA transporter 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 793.5 bits (2048), Expect = 9.7e-226
Identity = 407/455 (89.45%), Postives = 426/455 (93.63%), Query Frame = 0

Query: 6   AIISGDTMA--KENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLP 65
           A +SGD  A  KE+G A VQ TV +LDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLP
Sbjct: 5   ASLSGDEAAADKESGRAPVQSTV-ELDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLP 64

Query: 66  FAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPKWAI 125
           FAFRLLGWVGG ICLLF G VTFYAY+LLSLVLEHHA+RGSRLLRFRDMAT ILGPKWAI
Sbjct: 65  FAFRLLGWVGGSICLLFGGFVTFYAYNLLSLVLEHHAIRGSRLLRFRDMATYILGPKWAI 124

Query: 126 FYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPS 185
           FYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEG MQLY+FIIIFG LMLILAQIPS
Sbjct: 125 FYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGRMQLYEFIIIFGALMLILAQIPS 184

Query: 186 FHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVI 245
           FHS+RHINLISLTLSLAYSACVTAASLKLG+SKNAPPRDYSV+GSPVSQLFNAFNGISVI
Sbjct: 185 FHSMRHINLISLTLSLAYSACVTAASLKLGYSKNAPPRDYSVEGSPVSQLFNAFNGISVI 244

Query: 246 ATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLT 305
           AT YACGMLPEIQATLVAP+KGKMFKGLCLCY VIA TFLSVGIS YWTFG EAMGTVL+
Sbjct: 245 ATTYACGMLPEIQATLVAPVKGKMFKGLCLCYAVIAATFLSVGISGYWTFGKEAMGTVLS 304

Query: 306 NFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRL 365
           NFMS N LPSWL+IITNAFCL QVSAVAGTYLQPTNE  EK FADPNK+QFS+RNIVPRL
Sbjct: 305 NFMSNNLLPSWLLIITNAFCLLQVSAVAGTYLQPTNEVLEKIFADPNKNQFSIRNIVPRL 364

Query: 366 ISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWIN 425
           ISRSLSVVIATI+GAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKR ++YW+N
Sbjct: 365 ISRSLSVVIATIIGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRGYLYWLN 424

Query: 426 TLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           TLIV +SSVLA IGGVASIRQIVLDAKEYRLFANV
Sbjct: 425 TLIVVVSSVLAFIGGVASIRQIVLDAKEYRLFANV 458

BLAST of CSPI05G26550 vs. NCBI nr
Match: XP_008446788.1 (PREDICTED: GABA transporter 1-like [Cucumis melo] >KAA0034673.1 GABA transporter 1-like [Cucumis melo var. makuwa] >TYK09224.1 GABA transporter 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 781.2 bits (2016), Expect = 5.0e-222
Identity = 403/456 (88.38%), Postives = 421/456 (92.32%), Query Frame = 0

Query: 6   AIISGDTMA---KENGNAHVQLTVDQLDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSL 65
           A ISG   A   KE G A VQ T  +LDAGALFVLKSRGSWWHCGYHLTTSIVAP+LLSL
Sbjct: 5   APISGADQAAADKEKGGALVQPTA-ELDAGALFVLKSRGSWWHCGYHLTTSIVAPALLSL 64

Query: 66  PFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALRGSRLLRFRDMATNILGPKWA 125
           PFAFRLLGWVGG ICLL  GVVTFYAY LLSLVLEHHA++GSRLLRFRDMAT ILGPKWA
Sbjct: 65  PFAFRLLGWVGGSICLLLGGVVTFYAYLLLSLVLEHHAMQGSRLLRFRDMATYILGPKWA 124

Query: 126 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIP 185
           IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEG MQLYQFIIIFGTLMLILAQIP
Sbjct: 125 IFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNPEGEMQLYQFIIIFGTLMLILAQIP 184

Query: 186 SFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISV 245
           SFHSLRHINL+SLTLSLAYSA  TAASL LG+SKNAPPRDYS++GSPVSQLFNAFNGISV
Sbjct: 185 SFHSLRHINLVSLTLSLAYSASATAASLILGYSKNAPPRDYSLQGSPVSQLFNAFNGISV 244

Query: 246 IATAYACGMLPEIQATLVAPLKGKMFKGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVL 305
           IAT YACGMLPEIQATLVAP+KGKMFKGLCLCYTVIA TFLSVGIS YWTFGNEAMGTVL
Sbjct: 245 IATTYACGMLPEIQATLVAPVKGKMFKGLCLCYTVIAVTFLSVGISGYWTFGNEAMGTVL 304

Query: 306 TNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPR 365
           +NFM+ NSLPSWL+IITN FC  QVSAVAGTYLQPTNE FEK FADPNK+QFSMRNIVPR
Sbjct: 305 SNFMAHNSLPSWLLIITNIFCFLQVSAVAGTYLQPTNEVFEKIFADPNKNQFSMRNIVPR 364

Query: 366 LISRSLSVVIATIVGAMLPFFGDLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWI 425
           LISRSLSVV+A I+GAMLPFFGDLMALIGA GFIPLDFIMPM+FYNATFKPSK S IYW+
Sbjct: 365 LISRSLSVVVAIIIGAMLPFFGDLMALIGAFGFIPLDFIMPMIFYNATFKPSKHSLIYWV 424

Query: 426 NTLIVAISSVLAIIGGVASIRQIVLDAKEYRLFANV 459
           NTLIVA+SSVLAIIGGVASIRQIVLDAKEYRLFANV
Sbjct: 425 NTLIVAVSSVLAIIGGVASIRQIVLDAKEYRLFANV 459

BLAST of CSPI05G26550 vs. TAIR 10
Match: AT1G08230.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.6e-149
Identity = 252/434 (58.06%), Postives = 333/434 (76.73%), Query Frame = 0

Query: 29  LDAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYA 88
           +DAG+LFVLKS+G+WWHCG+HLTTSIVAP+LLSLP+AF+ LGW  GI CL+    VTFY+
Sbjct: 18  VDAGSLFVLKSKGTWWHCGFHLTTSIVAPALLSLPYAFKFLGWAAGISCLVGGAAVTFYS 77

Query: 89  YHLLSLVLEHHALRGSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQN 148
           Y LLSL LEHHA  G+R LRFRDMA +IL PKW  +YVGPIQ  VCYG V+A  L+GGQ 
Sbjct: 78  YTLLSLTLEHHASLGNRYLRFRDMAHHILSPKWGRYYVGPIQMAVCYGVVIANALLGGQC 137

Query: 149 LKYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAA 208
           LK +Y++  P G M+L++F+IIFG L+L+LAQ PSFHSLR+IN +SL L L YSA   AA
Sbjct: 138 LKAMYLVVQPNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAA 197

Query: 209 SLKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMF 268
           S+ +G   NAP +DY++ G P +++F  FN +++IAT Y  G++PEIQAT+ AP+KGKM 
Sbjct: 198 SIYIGKEPNAPEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMM 257

Query: 269 KGLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNS----LPSWLIIITNAFCL 328
           KGLC+CY V+  TF +V I+ YW FG +A G + TNF++  +    +P+W I + N F +
Sbjct: 258 KGLCMCYLVVIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTV 317

Query: 329 TQVSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFG 388
            Q+SAVA  YLQP N+  E   +DP K +FS+RN++PRL+ RSL VV+ATIV AMLPFFG
Sbjct: 318 LQLSAVAVVYLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFG 377

Query: 389 DLMALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQ 448
           D+ +L+GA GFIPLDF++P+VF+N TFKPSK+SFI+WINT+I  + S L +I  VA++RQ
Sbjct: 378 DVNSLLGAFGFIPLDFVLPVVFFNFTFKPSKKSFIFWINTVIAVVFSCLGVIAMVAAVRQ 437

Query: 449 IVLDAKEYRLFANV 459
           I++DA  Y+LFA+V
Sbjct: 438 IIIDANTYKLFADV 451

BLAST of CSPI05G26550 vs. TAIR 10
Match: AT5G41800.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 2.0e-112
Identity = 210/431 (48.72%), Postives = 287/431 (66.59%), Query Frame = 0

Query: 30  DAGALFVLKSRGSWWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAY 89
           DAGALFVL+S+G WWH G+HLTT+IV P++L+LP+AFR LGW  G +CL   G+VTFYAY
Sbjct: 18  DAGALFVLQSKGEWWHAGFHLTTAIVGPTILTLPYAFRGLGWWLGFVCLTTMGLVTFYAY 77

Query: 90  HLLSLVLEHHALRGSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNL 149
           +L+S VL+H    G R +RFR++A ++LG     + V  IQ  +  G  +  IL+ GQ L
Sbjct: 78  YLMSKVLDHCEKSGRRHIRFRELAADVLGSGLMFYVVIFIQTAINTGIGIGAILLAGQCL 137

Query: 150 KYIYVLCNPEGGMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAAS 209
             +Y    P+G ++LY+FI +   +M++L+Q+PSFHSLRHIN  SL LSL Y+  V  A 
Sbjct: 138 DIMYSSLFPQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINCASLLLSLGYTFLVVGAC 197

Query: 210 LKLGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMFK 269
           + LG SKNAP R+YS++ S   ++F+AF  IS+IA  +  G+LPEIQATL  P  GKM K
Sbjct: 198 INLGLSKNAPKREYSLEHSDSGKVFSAFTSISIIAAIFGNGILPEIQATLAPPATGKMLK 257

Query: 270 GLCLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNS---LPSWLIIITNAFCLTQ 329
           GL LCY+VI  TF S  IS YW FGN +   +L N M        P  +I +   F L Q
Sbjct: 258 GLLLCYSVIFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPTLAPIVVIGLAVIFVLLQ 317

Query: 330 VSAVAGTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDL 389
           + A+   Y Q   E  EK  AD  K  FS RN+VPRLI R+L +     + AMLPFFGD+
Sbjct: 318 LFAIGLVYSQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLYMAFCGFMAAMLPFFGDI 377

Query: 390 MALIGALGFIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIV 449
            A++GA GFIPLDF++PM+ YN T+KP++RSF YWIN  I+ + +   ++G  +SIR++V
Sbjct: 378 NAVVGAFGFIPLDFVLPMLLYNMTYKPTRRSFTYWINMTIMVVFTCAGLMGAFSSIRKLV 437

Query: 450 LDAKEYRLFAN 458
           LDA +++LF++
Sbjct: 438 LDANKFKLFSS 448

BLAST of CSPI05G26550 vs. TAIR 10
Match: AT1G48640.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-47
Identity = 124/421 (29.45%), Postives = 211/421 (50.12%), Query Frame = 0

Query: 43  WWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALR 102
           WW+  +H  T++V   +L LPF    LGW  GI  L+   ++T Y   L  +V  H  + 
Sbjct: 45  WWYSTFHNVTAMVGAGVLGLPFFMAQLGWGPGIAVLILSWIITLYT--LWQMVEMHEMVP 104

Query: 103 GSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIY-VLCNPEGG 162
           G R  R+ ++     G +  ++ + P Q  V  G  +  ++ GGQ+LK  + + C     
Sbjct: 105 GKRFDRYHELGQFAFGERLGLYIIVPQQIIVEVGVCIVYMVTGGQSLKKFHEIACQDCSP 164

Query: 163 MQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPPR 222
           ++L  FI+IF +   +L+ +P+F+S+  ++L++  +SL+YS     A+   G  ++    
Sbjct: 165 IRLSFFIMIFASSHFVLSHLPNFNSISGVSLVAAVMSLSYSTIAWTATAAKGVQEDV--- 224

Query: 223 DYSVK-GSPVSQLFNAFNGISVIATAYA-CGMLPEIQATLVA----PLKGKMFKGLCLCY 282
            Y  K G+  S + + F G+  IA AYA   ++ EIQAT+ +    P KG M++G+ + Y
Sbjct: 225 QYGYKSGTTASTVLSFFTGLGGIAFAYAGHNVVLEIQATIPSTPSNPSKGPMWRGVVVAY 284

Query: 283 TVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTYL 342
            V+A  +  V +  Y  FGN     VL N +     P W I   N F +  V      + 
Sbjct: 285 VVVALCYFPVALVGYGVFGN----AVLDNVLMSLETPVWAIATANLFVVMHVIGSYQIFA 344

Query: 343 QPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALGF 402
            P  +  E TF    K  F    ++ R I R++ V +   +G M+PFFG L+A  G   F
Sbjct: 345 MPVFDMVE-TFL-VKKLNFKPSTVL-RFIVRNVYVALTMFIGIMIPFFGGLLAFFGGFAF 404

Query: 403 IPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRLF 457
            P  + +P + +   +KP + S  +W N + + +  VL I+  +  +RQI++ +K+Y  F
Sbjct: 405 APTSYFLPCIMWLLIYKPKRFSLSWWTNWVCIVLGVVLMILSSIGGLRQIIIQSKDYSFF 453

BLAST of CSPI05G26550 vs. TAIR 10
Match: AT1G71680.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-45
Identity = 131/422 (31.04%), Postives = 211/422 (50.00%), Query Frame = 0

Query: 43  WWHCGYHLTTSIVAPSLLSLPFAFRLLGWVGGIICLLFCGVVTFYAYHLLSLVLEHHALR 102
           W++  +H  T++V   +L LPFA   LGW  G++ ++    +TFY+  L  +V  H A+ 
Sbjct: 39  WYYSAFHNVTAMVGAGVLGLPFAMSQLGWGPGLVAIIMSWAITFYS--LWQMVQLHEAVP 98

Query: 103 GSRLLRFRDMATNILGPKWAIFYVGPIQFGVCYGSVVAGILIGGQNLKYIYVLCNP--EG 162
           G RL R+ ++     GPK   + V P Q  V   S +   + GG++LK    L  P  E 
Sbjct: 99  GKRLDRYPELGQEAFGPKLGYWIVMPQQLLVQIASDIVYNVTGGKSLKKFVELLFPNLEH 158

Query: 163 GMQLYQFIIIFGTLMLILAQIPSFHSLRHINLISLTLSLAYSACVTAASLKLGFSKNAPP 222
             Q Y +I+ F  L L+L+Q P F+S++ ++L++  +S  YS   + AS+  G      P
Sbjct: 159 IRQTY-YILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGTEHR--P 218

Query: 223 RDYSVKGSPV-SQLFNAFNGISVIATAYA-CGMLPEIQATLVA----PLKGKMFKGLCLC 282
             Y V+G  V S +F+AFNGI  IA A+A   ++ EIQAT+ +    P K  M+KG+ + 
Sbjct: 219 STYGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVVVA 278

Query: 283 YTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVAGTY 342
           Y ++   +L V IS YW FG      VL +       P+WLI   N         V G+Y
Sbjct: 279 YIIVIICYLFVAISGYWAFGAHVEDDVLISL----ERPAWLIAAANFMVFIH---VIGSY 338

Query: 343 LQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIGALG 402
                  F+   +   K      +   RL++RS  V +  +V   +PFFG L+   G L 
Sbjct: 339 QVFAMIVFDTIESYLVKTLKFTPSTTLRLVARSTYVALICLVAVCIPFFGGLLGFFGGLV 398

Query: 403 FIPLDFIMPMVFYNATFKPSKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLDAKEYRL 457
           F    + +P + +    +P + S  +W + + +     +AI+  +  +R I+L A+ Y+L
Sbjct: 399 FSSTSYFLPCIIWLIMKRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILSARTYKL 448

BLAST of CSPI05G26550 vs. TAIR 10
Match: AT2G36590.1 (proline transporter 3 )

HSP 1 Score: 176.0 bits (445), Expect = 6.8e-44
Identity = 130/430 (30.23%), Postives = 212/430 (49.30%), Query Frame = 0

Query: 39  SRGSWWHCGYHLTTSIVAPSLLSLPFAFRL-LGWVGGIICLLFCGVVTFYAYHLLSLVLE 98
           S  SW+   + LTTSI +  +L       + LGW+GG++ L+    ++ YA    +LV +
Sbjct: 26  SSDSWFQAAFVLTTSINSAYVLGYSGTVMVPLGWIGGVVGLILATAISLYAN---TLVAK 85

Query: 99  HHALRGSRLLRFRDMATNILGPK-----WAIFYVGPIQFGVCYGSVVAGILIGGQNLKYI 158
            H   G R +R+RD+A  I G K     W + YV        +      I++ G  LK +
Sbjct: 86  LHEFGGKRHIRYRDLAGFIYGRKAYCLTWVLQYVNLFMINCGF------IILAGSALKAV 145

Query: 159 YVLCNPEGGMQLYQFIIIFGTLMLILA-QIPSFHSLRHINLISLTLSLAYSACVTAASLK 218
           YVL   +  M+L  FI I G +  + A  IP   +L     +S  LSL Y       S+K
Sbjct: 146 YVLFRDDHAMKLPHFIAIAGLICAVFAIGIPHLSALGIWLAVSTILSLIYIVVAIVLSVK 205

Query: 219 LGFSKNAPPRDYSVKGSPVSQLFNAFNGISVIATAYACGMLPEIQATLVAPLKGKMFKGL 278
            G    AP RDY ++GSP+S+LF      + +   +  GMLPEIQAT+  P+   M K L
Sbjct: 206 DGV--KAPSRDYEIQGSPLSKLFTITGAAATLVFVFNTGMLPEIQATVKQPVVKNMMKAL 265

Query: 279 CLCYTVIATTFLSVGISAYWTFGNEAMGTVLTNFMSQNSLPSWLIIITNAFCLTQVSAVA 338
              +TV      +V    YW +G+     +L N       P W+  + N   + Q     
Sbjct: 266 YFQFTVGVLPMFAVVFIGYWAYGSSTSPYLLNNVNG----PLWVKALANISAILQSVISL 325

Query: 339 GTYLQPTNEAFEKTFADPNKDQFSMRNIVPRLISRSLSVVIATIVGAMLPFFGDLMALIG 398
             +  PT E  +  F     +  +++N++ R+++R   + ++T++ A+LPF GD M+L G
Sbjct: 326 HIFASPTYEYMDTKFGIKG-NPLALKNLLFRIMARGGYIAVSTLLSALLPFLGDFMSLTG 385

Query: 399 ALGFIPLDFIMP-MVFYNATFKP--SKRSFIYWINTLIVAISSVLAIIGGVASIRQIVLD 458
           A+   PL FI+   ++Y A      + +   +W+N +  ++ SV A    +A++R I LD
Sbjct: 386 AVSTFPLTFILANHMYYKAKNNKLNTLQKLCHWLNVVFFSLMSVAA---AIAALRLIALD 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HW022.2e-14858.06GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1[more]
Q8L4X42.8e-11148.72Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV... [more]
Q9C7333.2e-4629.45Lysine histidine transporter-like 1 OS=Arabidopsis thaliana OX=3702 GN=At1g48640... [more]
Q9C9J02.3e-4431.04Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680... [more]
Q9SJP99.6e-4330.23Proline transporter 3 OS=Arabidopsis thaliana OX=3702 GN=PROT3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWN76.7e-257100.00Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G615820 PE... [more]
A0A5D3CBK11.0e-25298.25GABA transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BGK01.0e-25298.25GABA transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103489410 PE=3 SV=1[more]
A0A5D3CDF22.4e-22288.38GABA transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BGQ72.4e-22288.38GABA transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103489411 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142456.11.4e-256100.00GABA transporter 1 [Cucumis sativus] >KGN52211.1 hypothetical protein Csa_008337... [more]
XP_008446787.12.1e-25298.25PREDICTED: GABA transporter 1-like [Cucumis melo] >KAA0034672.1 GABA transporter... [more]
XP_038891456.11.1e-23290.87GABA transporter 1-like [Benincasa hispida][more]
XP_038891453.19.7e-22689.45GABA transporter 1-like isoform X1 [Benincasa hispida][more]
XP_008446788.15.0e-22288.38PREDICTED: GABA transporter 1-like [Cucumis melo] >KAA0034673.1 GABA transporter... [more]
Match NameE-valueIdentityDescription
AT1G08230.21.6e-14958.06Transmembrane amino acid transporter family protein [more]
AT5G41800.12.0e-11248.72Transmembrane amino acid transporter family protein [more]
AT1G48640.12.3e-4729.45Transmembrane amino acid transporter family protein [more]
AT1G71680.11.6e-4531.04Transmembrane amino acid transporter family protein [more]
AT2G36590.16.8e-4430.23proline transporter 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 40..441
e-value: 3.8E-64
score: 216.9
NoneNo IPR availablePANTHERPTHR48017OS05G0424000 PROTEIN-RELATEDcoord: 13..453
NoneNo IPR availablePANTHERPTHR48017:SF38GABA TRANSPORTER 1-LIKEcoord: 13..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G26550.1CSPI05G26550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006865 amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015293 symporter activity