CSPI05G25390 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G25390
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionacyl-acyl carrier protein thioesterase ATL3, chloroplastic-like
LocationChr5: 24675720 .. 24676642 (-)
RNA-Seq ExpressionCSPI05G25390
SyntenyCSPI05G25390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCCAATTCTTCAATTTTCACCTACTAAAACCAAATTCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACCATTTTTCTTCACCAAAACAAGAACCTCTGTTTCTCAAAAAGTTTCCATTAGGTACTCTGTTTTCTGTTCATCACCACTAACCAACAATTATATAAACTAAAAAAACCAACATTGTTGATTGACAGTCAAGGTTACAAGATGGATGGGTTCCAAGATTTTGAGCTCAAAGTTCGTGATTACGAGCTGGATCAATTCGGTGTAGTCAACAATGCTGTTTATGCAGATTATTGCTACCATGGTCAAGCTGATCTAAACTCGAAACTTCTGTTAAATTGATAAGGTAAGTTTTATAATTGTAATTAAACAGGTCGTCACGAATTGCTAGAAAAGATGGGAGTGAGTCGGGATGCAATTACTCGGAGAGGGGAATCATTAGCGCTATCAGATTTGTCCATGAAATTCCTTTCATCACTTGGAGTATGGATTAATTAACCATAACATAACTAACCCATTTATTACTATAAATTTTGGTTTAATCAAACTACAAAAACTTATAGAATTATGATCAAATATAGAGTGGAGAGAGATTTGTTCTTAAAGTGAGGGTGTTGGGAATCTCAAACGTTCGGATCTATATTCATCACTTGATTTTCAAGCTTCCAAATATGGAGGTTAGTAGTAGTGAGTAATGAAATTAAATTAAACATACCTAGATTGTTGATTATTCAATTAGTGTTTGTATGCGATTGTATTGCAATTTGCAAACAGGCAATCTTAGATGCAAAGGCTACAGCAGTATGGATTAATAAAAACAATCGTCCAGTTCGAATCCCTCAGGAGTTTTTCTCAAAATTTGCTGATTATGTTGGGAAACAACAACATCACTTCAAAACAGAACAAAATTAG

mRNA sequence

ATGAATCCAATTCTTCAATTTTCACCTACTAAAACCAAATTCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACCATTTTTCTTCACCAAAACAAGAACCTCTGTTTCTCAAAAAGTTTCCATTAGTCAAGGTTACAAGATGGATGGGTTCCAAGATTTTGAGCTCAAAGTTCGTGATTACGAGCTGGATCAATTCGGTGTAGTCAACAATGCTGTTTATGCAGATTATTGCTACCATGGTCGTCACGAATTGCTAGAAAAGATGGGAGTGAGTCGGGATGCAATTACTCGGAGAGGGGAATCATTAGCGCTATCAGATTTGTCCATGAAATTCCTTTCATCACTTGGAAGTGGAGAGAGATTTGTTCTTAAAGTGAGGGTGTTGGGAATCTCAAACGTTCGGATCTATATTCATCACTTGATTTTCAAGCTTCCAAATATGGAGGCAATCTTAGATGCAAAGGCTACAGCAGTATGGATTAATAAAAACAATCGTCCAGTTCGAATCCCTCAGGAGTTTTTCTCAAAATTTGCTGATTATGTTGGGAAACAACAACATCACTTCAAAACAGAACAAAATTAG

Coding sequence (CDS)

ATGAATCCAATTCTTCAATTTTCACCTACTAAAACCAAATTCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCACCATTTTTCTTCACCAAAACAAGAACCTCTGTTTCTCAAAAAGTTTCCATTAGTCAAGGTTACAAGATGGATGGGTTCCAAGATTTTGAGCTCAAAGTTCGTGATTACGAGCTGGATCAATTCGGTGTAGTCAACAATGCTGTTTATGCAGATTATTGCTACCATGGTCGTCACGAATTGCTAGAAAAGATGGGAGTGAGTCGGGATGCAATTACTCGGAGAGGGGAATCATTAGCGCTATCAGATTTGTCCATGAAATTCCTTTCATCACTTGGAAGTGGAGAGAGATTTGTTCTTAAAGTGAGGGTGTTGGGAATCTCAAACGTTCGGATCTATATTCATCACTTGATTTTCAAGCTTCCAAATATGGAGGCAATCTTAGATGCAAAGGCTACAGCAGTATGGATTAATAAAAACAATCGTCCAGTTCGAATCCCTCAGGAGTTTTTCTCAAAATTTGCTGATTATGTTGGGAAACAACAACATCACTTCAAAACAGAACAAAATTAG

Protein sequence

MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFADYVGKQQHHFKTEQN*
Homology
BLAST of CSPI05G25390 vs. ExPASy Swiss-Prot
Match: S4TE15 (Acyl-acyl carrier protein thioesterase TE3, chloroplastic OS=Humulus lupulus OX=3486 GN=TE3 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.0e-46
Identity = 101/185 (54.59%), Postives = 125/185 (67.57%), Query Frame = 0

Query: 17  SSSSSSSSSPFFFTKTRTSVSQKV---------------SISQGYKMDGFQDFELKVRDY 76
           SSSSSSS+S   F  TR  +  ++                I  G  M  F + ELKVRDY
Sbjct: 22  SSSSSSSASSVAFPVTRLLIPPRLRVLPNPRRRCSALPFDIRGGKGMSEFYEVELKVRDY 81

Query: 77  ELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGER 136
           ELDQ+GVVNNAVYA YC HGRHELLE  G+S DA+ R G++LALS+LS+KFL+ L SG++
Sbjct: 82  ELDQYGVVNNAVYASYCQHGRHELLESFGLSCDAVARNGDALALSELSLKFLAPLRSGDK 141

Query: 137 FVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFADY 187
           FV+KVR+ G S  R+Y  HLIFKLPN E ILDAK TAVW++KN RPVRIP E  SK   +
Sbjct: 142 FVVKVRLSGSSAARLYFDHLIFKLPNQEPILDAKGTAVWLDKNYRPVRIPPEVRSKLVQF 201

BLAST of CSPI05G25390 vs. ExPASy Swiss-Prot
Match: Q8W583 (Acyl-acyl carrier protein thioesterase ATL3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT3 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 7.0e-43
Identity = 83/138 (60.14%), Postives = 102/138 (73.91%), Query Frame = 0

Query: 45  GYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLA 104
           G  M  F + ELKVRDYELDQFGVVNNAVYA+YC HGRHE LE +G++ D + R GE+LA
Sbjct: 47  GKGMSEFHEVELKVRDYELDQFGVVNNAVYANYCQHGRHEFLESIGINCDEVARSGEALA 106

Query: 105 LSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKN 164
           +S+L+MKFLS L SG++FV+K R+ G S  RIY  H IFKLPN E IL+AK  AVW++  
Sbjct: 107 ISELTMKFLSPLRSGDKFVVKARISGTSAARIYFDHFIFKLPNQEPILEAKGIAVWLDNK 166

Query: 165 NRPVRIPQEFFSKFADYV 183
            RPVRIP    SKF  ++
Sbjct: 167 YRPVRIPSSIRSKFVHFL 184

BLAST of CSPI05G25390 vs. ExPASy Swiss-Prot
Match: F4HX80 (Acyl-acyl carrier protein thioesterase ATL4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT4 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 6.5e-41
Identity = 79/143 (55.24%), Postives = 103/143 (72.03%), Query Frame = 0

Query: 40  VSISQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRR 99
           + +  G +M  F + ELKVRDYELDQFGVVNNAVYA+YC HG HE LE +G++ D + R 
Sbjct: 40  LDLKGGKEMSEFHEVELKVRDYELDQFGVVNNAVYANYCQHGMHEFLESIGINCDEVARS 99

Query: 100 GESLALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAV 159
           GE+LA+S+L+M FL+ L SG++FV+KV +   S  RIY  H I KLPN E IL+AKAT V
Sbjct: 100 GEALAISELTMNFLAPLRSGDKFVVKVNISRTSAARIYFDHSILKLPNQEVILEAKATVV 159

Query: 160 WINKNNRPVRIPQEFFSKFADYV 183
           W++  +RPVRIP    SKF  ++
Sbjct: 160 WLDNKHRPVRIPSSIRSKFVHFL 182

BLAST of CSPI05G25390 vs. ExPASy Swiss-Prot
Match: Q9C7I8 (Acyl-acyl carrier protein thioesterase ATL2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT2 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-38
Identity = 75/132 (56.82%), Postives = 98/132 (74.24%), Query Frame = 0

Query: 50  GFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLS 109
           GF + ELKVRDYELDQFGVVNNAVYA+YC HGRHE ++ +G++ + ++R G +LA+ +L+
Sbjct: 50  GFHEIELKVRDYELDQFGVVNNAVYANYCQHGRHEFMDSIGINCNEVSRSGGALAIPELT 109

Query: 110 MKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVR 169
           +KFL+ L SG RFV+K R+ GIS VRIY    IFKLPN E IL+AK TAVW++   RP R
Sbjct: 110 IKFLAPLRSGCRFVVKTRISGISLVRIYFEQFIFKLPNQEPILEAKGTAVWLDNKYRPTR 169

Query: 170 IPQEFFSKFADY 182
           +P    S F  +
Sbjct: 170 VPSHVRSYFGHF 181

BLAST of CSPI05G25390 vs. ExPASy Swiss-Prot
Match: Q9C7I5 (Acyl-acyl carrier protein thioesterase ATL1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT1 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.0e-38
Identity = 86/168 (51.19%), Postives = 113/168 (67.26%), Query Frame = 0

Query: 23  SSSPFFFTKTRTSVSQKVSI--SQGYK-MDGFQDFELKVRDYELDQFGVVNNAVYADYCY 82
           SS P       T   + +S    QG K M+G  + ELKVRDYELDQFGVVNNAVYA+YC 
Sbjct: 20  SSRPLILPLRSTKTFKPLSCFKQQGGKGMNGVHEIELKVRDYELDQFGVVNNAVYANYCQ 79

Query: 83  HGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIH 142
           HG+HE +E +G++ D ++R GE+LA+S+L++KFL+ L SG +FV+K R+ G S  RIY  
Sbjct: 80  HGQHEFMETIGINCDEVSRSGEALAVSELTIKFLAPLRSGCKFVVKTRISGTSMTRIYFE 139

Query: 143 HLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFADYVGKQQH 188
             IFKLPN E IL+AK  AVW++K  RPV IP    S F  +  ++QH
Sbjct: 140 QFIFKLPNQEPILEAKGMAVWLDKRYRPVCIPSYIRSNFGHF--QRQH 185

BLAST of CSPI05G25390 vs. ExPASy TrEMBL
Match: A0A0A0KR74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609790 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.5e-99
Identity = 190/194 (97.94%), Postives = 191/194 (98.45%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNPILQFSPTKTKFP SSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDG+QDFELKVRD
Sbjct: 1   MNPILQFSPTKTKFP-SSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGYQDFELKVRD 60

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSL SGE
Sbjct: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLRSGE 120

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV GISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD
Sbjct: 121 RFVLKVRVFGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180

Query: 181 YVGKQQHHFKTEQN 195
           YVGKQQHHFKTEQN
Sbjct: 181 YVGKQQHHFKTEQN 193

BLAST of CSPI05G25390 vs. ExPASy TrEMBL
Match: A0A5A7SZ57 (Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G005830 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.3e-88
Identity = 168/196 (85.71%), Postives = 184/196 (93.88%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNP++QFSPTKTKFP   SSSSSSSPFFF+KT+TSVSQ VSISQGYKMDGFQDFELKVRD
Sbjct: 9   MNPMIQFSPTKTKFP---SSSSSSSPFFFSKTKTSVSQTVSISQGYKMDGFQDFELKVRD 68

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RGESLALSDLSMKFLS L SG+
Sbjct: 69  YELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITERGESLALSDLSMKFLSPLRSGD 128

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV+G+S VRIY+HHLIFKLPNMEAILDAKATAVW+NK+NRPVR+P+E  SKFAD
Sbjct: 129 RFVLKVRVVGVSKVRIYVHHLIFKLPNMEAILDAKATAVWVNKSNRPVRLPEELLSKFAD 188

Query: 181 YVG--KQQHHFKTEQN 195
           YVG  +QQHHFKTEQ+
Sbjct: 189 YVGEQRQQHHFKTEQH 201

BLAST of CSPI05G25390 vs. ExPASy TrEMBL
Match: A0A1S3BGA0 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489291 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.3e-88
Identity = 168/196 (85.71%), Postives = 184/196 (93.88%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNP++QFSPTKTKFP   SSSSSSSPFFF+KT+TSVSQ VSISQGYKMDGFQDFELKVRD
Sbjct: 9   MNPMIQFSPTKTKFP---SSSSSSSPFFFSKTKTSVSQTVSISQGYKMDGFQDFELKVRD 68

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RGESLALSDLSMKFLS L SG+
Sbjct: 69  YELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITERGESLALSDLSMKFLSPLRSGD 128

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV+G+S VRIY+HHLIFKLPNMEAILDAKATAVW+NK+NRPVR+P+E  SKFAD
Sbjct: 129 RFVLKVRVVGVSKVRIYVHHLIFKLPNMEAILDAKATAVWVNKSNRPVRLPEELLSKFAD 188

Query: 181 YVG--KQQHHFKTEQN 195
           YVG  +QQHHFKTEQ+
Sbjct: 189 YVGEQRQQHHFKTEQH 201

BLAST of CSPI05G25390 vs. ExPASy TrEMBL
Match: A0A5D3CCR4 (Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001110 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 8.2e-71
Identity = 133/154 (86.36%), Postives = 146/154 (94.81%), Query Frame = 0

Query: 43  SQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGES 102
           +QGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RGES
Sbjct: 555 NQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITERGES 614

Query: 103 LALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWIN 162
           LALSDLSMKFLS L SG+RFVLKVRV+G+S VRIY+HHLIFKLPNMEAILDAKATAVW+N
Sbjct: 615 LALSDLSMKFLSPLRSGDRFVLKVRVVGVSKVRIYVHHLIFKLPNMEAILDAKATAVWVN 674

Query: 163 KNNRPVRIPQEFFSKFADYVG--KQQHHFKTEQN 195
           K+NRPVR+P+E  SKFADYVG  +QQHHFKTEQ+
Sbjct: 675 KSNRPVRLPEELLSKFADYVGEQRQQHHFKTEQH 708

BLAST of CSPI05G25390 vs. ExPASy TrEMBL
Match: A0A6J1HST6 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467460 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.9e-51
Identity = 111/180 (61.67%), Postives = 132/180 (73.33%), Query Frame = 0

Query: 3   PILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRDYE 62
           PI + SPT  +  S S             TR+S+S    +  G  MDGF D ELKVRDYE
Sbjct: 32  PISRPSPTSLRLRSPS------------VTRSSISSSFQLKAGKGMDGFLDVELKVRDYE 91

Query: 63  LDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGERF 122
           LDQFGVVNNAVYA YC HGRHELLE +G+S DA+ R G++LALS+LS+KFL+ L SG+RF
Sbjct: 92  LDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDRF 151

Query: 123 VLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFADYV 182
           V+KVR+ G+S  R YI H IFKLPNME IL+AKATAVW+NK+ RPVRIPQEF SKFA +V
Sbjct: 152 VVKVRISGMSAARFYIDHFIFKLPNMEPILEAKATAVWLNKSYRPVRIPQEFQSKFAQFV 199

BLAST of CSPI05G25390 vs. NCBI nr
Match: XP_011655778.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic isoform X1 [Cucumis sativus] >XP_031741192.1 acyl-acyl carrier protein thioesterase ATL3, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 190/194 (97.94%), Postives = 191/194 (98.45%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNPILQFSPTKTKFP SSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDG+QDFELKVRD
Sbjct: 9   MNPILQFSPTKTKFP-SSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGYQDFELKVRD 68

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSL SGE
Sbjct: 69  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLRSGE 128

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV GISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD
Sbjct: 129 RFVLKVRVFGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 188

Query: 181 YVGKQQHHFKTEQN 195
           YVGKQQHHFKTEQN
Sbjct: 189 YVGKQQHHFKTEQN 201

BLAST of CSPI05G25390 vs. NCBI nr
Match: XP_008446608.1 (PREDICTED: acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucumis melo] >KAA0034567.1 acyl-acyl carrier protein thioesterase ATL3 [Cucumis melo var. makuwa])

HSP 1 Score: 334.0 bits (855), Expect = 8.9e-88
Identity = 168/196 (85.71%), Postives = 184/196 (93.88%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNP++QFSPTKTKFP   SSSSSSSPFFF+KT+TSVSQ VSISQGYKMDGFQDFELKVRD
Sbjct: 9   MNPMIQFSPTKTKFP---SSSSSSSPFFFSKTKTSVSQTVSISQGYKMDGFQDFELKVRD 68

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RGESLALSDLSMKFLS L SG+
Sbjct: 69  YELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITERGESLALSDLSMKFLSPLRSGD 128

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV+G+S VRIY+HHLIFKLPNMEAILDAKATAVW+NK+NRPVR+P+E  SKFAD
Sbjct: 129 RFVLKVRVVGVSKVRIYVHHLIFKLPNMEAILDAKATAVWVNKSNRPVRLPEELLSKFAD 188

Query: 181 YVG--KQQHHFKTEQN 195
           YVG  +QQHHFKTEQ+
Sbjct: 189 YVGEQRQQHHFKTEQH 201

BLAST of CSPI05G25390 vs. NCBI nr
Match: KAE8648792.1 (hypothetical protein Csa_009266 [Cucumis sativus])

HSP 1 Score: 302.8 bits (774), Expect = 2.2e-78
Identity = 148/152 (97.37%), Postives = 150/152 (98.68%), Query Frame = 0

Query: 43  SQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGES 102
           +QGYKMDG+QDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGES
Sbjct: 555 NQGYKMDGYQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGES 614

Query: 103 LALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWIN 162
           LALSDLSMKFLSSL SGERFVLKVRV GISNVRIYIHHLIFKLPNMEAILDAKATAVWIN
Sbjct: 615 LALSDLSMKFLSSLRSGERFVLKVRVFGISNVRIYIHHLIFKLPNMEAILDAKATAVWIN 674

Query: 163 KNNRPVRIPQEFFSKFADYVGKQQHHFKTEQN 195
           KNNRPVRIPQEFFSKFADYVGKQQHHFKTEQN
Sbjct: 675 KNNRPVRIPQEFFSKFADYVGKQQHHFKTEQN 706

BLAST of CSPI05G25390 vs. NCBI nr
Match: XP_038893060.1 (acyl-acyl carrier protein thioesterase ATL4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 286.2 bits (731), Expect = 2.1e-73
Identity = 149/193 (77.20%), Postives = 165/193 (85.49%), Query Frame = 0

Query: 1   MNPILQFSPTKTKFPSSSSSSSSSSPFFFTKTRTSVSQKVSISQGYKMDGFQDFELKVRD 60
           MNP++QFSPTK KFP       SSSP F TK  TSVSQ  S SQ YKMD FQ+FELKVRD
Sbjct: 9   MNPMIQFSPTKPKFP-------SSSPIFCTK--TSVSQTFSNSQSYKMDWFQEFELKVRD 68

Query: 61  YELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGE 120
           YELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RG+SLALS+LS+ FLS L SG+
Sbjct: 69  YELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITGRGDSLALSELSLNFLSPLRSGD 128

Query: 121 RFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFAD 180
           RFVLKVRV G S VRIYI HLIFKLPNMEAIL+AKATAVW+NKNNRPVRIP+E+ +KFA+
Sbjct: 129 RFVLKVRVSGTSRVRIYIQHLIFKLPNMEAILEAKATAVWVNKNNRPVRIPEEYMAKFAE 188

Query: 181 YVGKQQHHFKTEQ 194
           +VG  QHHFKTEQ
Sbjct: 189 FVG-DQHHFKTEQ 191

BLAST of CSPI05G25390 vs. NCBI nr
Match: TYK09120.1 (acyl-acyl carrier protein thioesterase ATL3 [Cucumis melo var. makuwa])

HSP 1 Score: 276.6 bits (706), Expect = 1.7e-70
Identity = 133/154 (86.36%), Postives = 146/154 (94.81%), Query Frame = 0

Query: 43  SQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGES 102
           +QGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLE++GVSRDAIT RGES
Sbjct: 555 NQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLERIGVSRDAITERGES 614

Query: 103 LALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWIN 162
           LALSDLSMKFLS L SG+RFVLKVRV+G+S VRIY+HHLIFKLPNMEAILDAKATAVW+N
Sbjct: 615 LALSDLSMKFLSPLRSGDRFVLKVRVVGVSKVRIYVHHLIFKLPNMEAILDAKATAVWVN 674

Query: 163 KNNRPVRIPQEFFSKFADYVG--KQQHHFKTEQN 195
           K+NRPVR+P+E  SKFADYVG  +QQHHFKTEQ+
Sbjct: 675 KSNRPVRLPEELLSKFADYVGEQRQQHHFKTEQH 708

BLAST of CSPI05G25390 vs. TAIR 10
Match: AT1G68260.1 (Thioesterase superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 5.0e-44
Identity = 83/138 (60.14%), Postives = 102/138 (73.91%), Query Frame = 0

Query: 45  GYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLA 104
           G  M  F + ELKVRDYELDQFGVVNNAVYA+YC HGRHE LE +G++ D + R GE+LA
Sbjct: 47  GKGMSEFHEVELKVRDYELDQFGVVNNAVYANYCQHGRHEFLESIGINCDEVARSGEALA 106

Query: 105 LSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKN 164
           +S+L+MKFLS L SG++FV+K R+ G S  RIY  H IFKLPN E IL+AK  AVW++  
Sbjct: 107 ISELTMKFLSPLRSGDKFVVKARISGTSAARIYFDHFIFKLPNQEPILEAKGIAVWLDNK 166

Query: 165 NRPVRIPQEFFSKFADYV 183
            RPVRIP    SKF  ++
Sbjct: 167 YRPVRIPSSIRSKFVHFL 184

BLAST of CSPI05G25390 vs. TAIR 10
Match: AT1G68280.1 (Thioesterase superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 4.6e-42
Identity = 79/143 (55.24%), Postives = 103/143 (72.03%), Query Frame = 0

Query: 40  VSISQGYKMDGFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRR 99
           + +  G +M  F + ELKVRDYELDQFGVVNNAVYA+YC HG HE LE +G++ D + R 
Sbjct: 40  LDLKGGKEMSEFHEVELKVRDYELDQFGVVNNAVYANYCQHGMHEFLESIGINCDEVARS 99

Query: 100 GESLALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAV 159
           GE+LA+S+L+M FL+ L SG++FV+KV +   S  RIY  H I KLPN E IL+AKAT V
Sbjct: 100 GEALAISELTMNFLAPLRSGDKFVVKVNISRTSAARIYFDHSILKLPNQEVILEAKATVV 159

Query: 160 WINKNNRPVRIPQEFFSKFADYV 183
           W++  +RPVRIP    SKF  ++
Sbjct: 160 WLDNKHRPVRIPSSIRSKFVHFL 182

BLAST of CSPI05G25390 vs. TAIR 10
Match: AT1G35250.1 (Thioesterase superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 9.7e-40
Identity = 75/132 (56.82%), Postives = 98/132 (74.24%), Query Frame = 0

Query: 50  GFQDFELKVRDYELDQFGVVNNAVYADYCYHGRHELLEKMGVSRDAITRRGESLALSDLS 109
           GF + ELKVRDYELDQFGVVNNAVYA+YC HGRHE ++ +G++ + ++R G +LA+ +L+
Sbjct: 50  GFHEIELKVRDYELDQFGVVNNAVYANYCQHGRHEFMDSIGINCNEVSRSGGALAIPELT 109

Query: 110 MKFLSSLGSGERFVLKVRVLGISNVRIYIHHLIFKLPNMEAILDAKATAVWINKNNRPVR 169
           +KFL+ L SG RFV+K R+ GIS VRIY    IFKLPN E IL+AK TAVW++   RP R
Sbjct: 110 IKFLAPLRSGCRFVVKTRISGISLVRIYFEQFIFKLPNQEPILEAKGTAVWLDNKYRPTR 169

Query: 170 IPQEFFSKFADY 182
           +P    S F  +
Sbjct: 170 VPSHVRSYFGHF 181

BLAST of CSPI05G25390 vs. TAIR 10
Match: AT1G35290.1 (Thioesterase superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 2.2e-39
Identity = 86/168 (51.19%), Postives = 113/168 (67.26%), Query Frame = 0

Query: 23  SSSPFFFTKTRTSVSQKVSI--SQGYK-MDGFQDFELKVRDYELDQFGVVNNAVYADYCY 82
           SS P       T   + +S    QG K M+G  + ELKVRDYELDQFGVVNNAVYA+YC 
Sbjct: 20  SSRPLILPLRSTKTFKPLSCFKQQGGKGMNGVHEIELKVRDYELDQFGVVNNAVYANYCQ 79

Query: 83  HGRHELLEKMGVSRDAITRRGESLALSDLSMKFLSSLGSGERFVLKVRVLGISNVRIYIH 142
           HG+HE +E +G++ D ++R GE+LA+S+L++KFL+ L SG +FV+K R+ G S  RIY  
Sbjct: 80  HGQHEFMETIGINCDEVSRSGEALAVSELTIKFLAPLRSGCKFVVKTRISGTSMTRIYFE 139

Query: 143 HLIFKLPNMEAILDAKATAVWINKNNRPVRIPQEFFSKFADYVGKQQH 188
             IFKLPN E IL+AK  AVW++K  RPV IP    S F  +  ++QH
Sbjct: 140 QFIFKLPNQEPILEAKGMAVWLDKRYRPVCIPSYIRSNFGHF--QRQH 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
S4TE153.0e-4654.59Acyl-acyl carrier protein thioesterase TE3, chloroplastic OS=Humulus lupulus OX=... [more]
Q8W5837.0e-4360.14Acyl-acyl carrier protein thioesterase ATL3, chloroplastic OS=Arabidopsis thalia... [more]
F4HX806.5e-4155.24Acyl-acyl carrier protein thioesterase ATL4, chloroplastic OS=Arabidopsis thalia... [more]
Q9C7I81.4e-3856.82Acyl-acyl carrier protein thioesterase ATL2, chloroplastic OS=Arabidopsis thalia... [more]
Q9C7I53.0e-3851.19Acyl-acyl carrier protein thioesterase ATL1, chloroplastic OS=Arabidopsis thalia... [more]
Match NameE-valueIdentityDescription
A0A0A0KR745.5e-9997.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609790 PE=4 SV=1[more]
A0A5A7SZ574.3e-8885.71Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BGA04.3e-8885.71acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucumis melo ... [more]
A0A5D3CCR48.2e-7186.36Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1HST61.9e-5161.67acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
XP_011655778.11.1e-9897.94acyl-acyl carrier protein thioesterase ATL3, chloroplastic isoform X1 [Cucumis s... [more]
XP_008446608.18.9e-8885.71PREDICTED: acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucu... [more]
KAE8648792.12.2e-7897.37hypothetical protein Csa_009266 [Cucumis sativus][more]
XP_038893060.12.1e-7377.20acyl-acyl carrier protein thioesterase ATL4, chloroplastic-like [Benincasa hispi... [more]
TYK09120.11.7e-7086.36acyl-acyl carrier protein thioesterase ATL3 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G68260.15.0e-4460.14Thioesterase superfamily protein [more]
AT1G68280.14.6e-4255.24Thioesterase superfamily protein [more]
AT1G35250.19.7e-4056.82Thioesterase superfamily protein [more]
AT1G35290.12.2e-3951.19Thioesterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 35..182
e-value: 5.9E-29
score: 102.8
NoneNo IPR availablePFAMPF132794HBT_2coord: 61..176
e-value: 3.1E-9
score: 37.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR31793:SF27ACYL-ACYL CARRIER PROTEIN THIOESTERASE ATL1, CHLOROPLASTIC-RELATEDcoord: 14..185
NoneNo IPR availablePANTHERPTHR317934-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBERcoord: 14..185
NoneNo IPR availableCDDcd005864HBTcoord: 54..162
e-value: 1.52557E-24
score: 90.7432
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 48..188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G25390.1CSPI05G25390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity