CSPI05G25150 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G25150
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Heavy metal-associated domain containing protein
Location	Chr5: 24529544 .. 24531209 (-)
RNA-Seq Expression	CSPI05G25150
Synteny	CSPI05G25150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGCAACCTCGAAGGAATCATCATCCTTTCCTTGCGAGAAGAAAAAATTAGGCAAAGCCCTTTGCGGAAAGCAGCTGTGTGATTGTGAAACCAACCATCTTTGTCAACTCTTTCTCATCATCATCATCATTTCACTCTCACGGCTCCCCTTTTACTTACTCTCCATTATCTTCCTCCTCTCTTTTCCCAACCACTTATATAATCATCCAATCCCCTTTTTTCACAATAACTCACACATAAAAATCTTCTTCCTTTGAATCGGAGGCGGAGCATGGGCGCGGTGGTGGTCGGAATCGGAGTTCGGAAGAAAAGGATTTTTCCTCATGCTTCTAGTCTTGCTTCCATTGAGTCCTTAACTCTGCCTCTTGTCTCTGCTTTTTCCTCTGTTCCTCTTGCATTTGTTCTGACGTTGTCTTCTTGTGGCTCTAATTTAATGGCGGACGATTGTTGATTTTCAGGTTCAGGAAATTGTGTTGACTGCCGATATTGGTTGTGCTGAGTGTCAGAAGAAACTGGCCAATATACTCTCCAAAATGAGCGGTGACATTCTACTCTGTTTTTGGGGAACTTCGTTCCATCTTAAGTATTTTTTTTTATTTTGTTCATGTATGGAATCTGATGTTTTGGCTTTGTTTTAAGACTGTTTTTTGGGAAAGTATCGGGTATGGTTTGAGAATTATTTAGGCCTGTTTGTAAGTTTAGATTTTAATATCCATATATGTGTTTTAGAATTTAACTTGGGTTTATTCAATAAATTTGAAATTCGTAGTTAAAAATTAGTGGGTAAACAAACATAAATGAATAAAATCCTTATCAAAGCCAAAGGGAACTTTAGAAAATTACTATCTTTACCTTTTTGTTTTAGATTCTTTAATCTACCTTCTAGAGGTGTTTTATTATAAAAGTTTTGATTAAGGCTTGTTTTAGTTCAAAATAAAATAGTTAACTTTGGCTGTATGGATTTCAGCTTTTCACTATCAAAGTAGGCTTTGAAAAATCATTTGTATTTATTTAGAAAGATGAAAACGTTGATACATGCTGATGGAAAAACAAACAAAGCTTTTAAGAAACCAATGGTTGATTATGATATAATTGGAGTATCATTGATTTGATGAATTTCGGTTGGGTATAGATACAGAGTCTGTGGTGGTGAATTTGTTGGACAAGAAAGTGATATTGACTCGAAGATCGCAAATTCCATCAAGAGTTTCAACAATCAGGAGATTGTTTGCTTCTCCTTGCAGATAACTATGGTGGAATATTTCTCACGTTGTTTAGTTTCAAAGAAAAAAAGTTTAAGAGACTGCCTTTGTATATTTTTCTTTCCAGATGAAAATATGTTTGGAAAGAATATAAATATTGCCTTTTGATGCAACAAAATGGGCGTACTACTTCAAAAGATGTTATTTGTGGAAGTTGAGCACACTTTGAAGGGCATTGAAGGGCATGTGTTAGCTACAAACTTTATTTTAGTGTAAAGTAATCAAATATTTGAGAAAATTTAGAAATGCAAAGGTATTGTAATAAATGTTGGGGTGGGGATTTGAACCCGATACCTCTTATCCACGTGAACATACATGTGCCCGTTGAGCTAAGTTTATGTTGACTATTTAACTTATATTTATACTGGCTATTGATAAAATTATATAAAATTGTTACAAGCTA

mRNA sequence

GAAGCAACCTCGAAGGAATCATCATCCTTTCCTTGCGAGAAGAAAAAATTAGGCAAAGCCCTTTGCGGAAAGCAGCTGTGTGATTGTGAAACCAACCATCTTTGTCAACTCTTTCTCATCATCATCATCATTTCACTCTCACGGCTCCCCTTTTACTTACTCTCCATTATCTTCCTCCTCTCTTTTCCCAACCACTTATATAATCATCCAATCCCCTTTTTTCACAATAACTCACACATAAAAATCTTCTTCCTTTGAATCGGAGGCGGAGCATGGGCGCGGTGGTGGTCGGAATCGGAGTTCGGAAGAAAAGGATTTTTCCTCATGCTTCTAGTCTTGCTTCCATTGAGTCCTTAACTCTGCCTCTTGTTCAGGAAATTGTGTTGACTGCCGATATTGGTTGTGCTGAGTGTCAGAAGAAACTGGCCAATATACTCTCCAAAATGAGCGATACAGAGTCTGTGGTGGTGAATTTGTTGGACAAGAAAGTGATATTGACTCGAAGATCGCAAATTCCATCAAGAGTTTCAACAATCAGGAGATTGTTTGCTTCTCCTTGCAGATAACTATGGTGGAATATTTCTCACGTTGTTTAGTTTCAAAGAAAAAAAGTTTAAGAGACTGCCTTTGTATATTTTTCTTTCCAGATGAAAATATGTTTGGAAAGAATATAAATATTGCCTTTTGATGCAACAAAATGGGCGTACTACTTCAAAAGATGTTATTTGTGGAAGTTGAGCACACTTTGAAGGGCATTGAAGGGCATGTGTTAGCTACAAACTTTATTTTAGTGTAAAGTAATCAAATATTTGAGAAAATTTAGAAATGCAAAGGTATTGTAATAAATGTTGGGGTGGGGATTTGAACCCGATACCTCTTATCCACGTGAACATACATGTGCCCGTTGAGCTAAGTTTATGTTGACTATTTAACTTATATTTATACTGGCTATTGATAAAATTATATAAAATTGTTACAAGCTA

Coding sequence (CDS)

ATGGGCGCGGTGGTGGTCGGAATCGGAGTTCGGAAGAAAAGGATTTTTCCTCATGCTTCTAGTCTTGCTTCCATTGAGTCCTTAACTCTGCCTCTTGTTCAGGAAATTGTGTTGACTGCCGATATTGGTTGTGCTGAGTGTCAGAAGAAACTGGCCAATATACTCTCCAAAATGAGCGATACAGAGTCTGTGGTGGTGAATTTGTTGGACAAGAAAGTGATATTGACTCGAAGATCGCAAATTCCATCAAGAGTTTCAACAATCAGGAGATTGTTTGCTTCTCCTTGCAGATAA

Protein sequence

MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR*

Homology

[Close]

BLAST of CSPI05G25150 vs. ExPASy TrEMBL
Match: A0A0A0KT62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608570 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-42
Identity = 96/97 (98.97%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSD 60
          MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKM+D
Sbjct: 1  MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMND 60

Query: 61 TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR 98
          TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR
Sbjct: 61 TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR 97

[Close]

BLAST of CSPI05G25150 vs. ExPASy TrEMBL
Match: A0A6J1HUG9 (uncharacterized protein LOC111467567 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467567 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-31
Identity = 79/96 (82.29%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 3   AVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTE 62
           A   GIGVRK +IFPHASSLASIESL+LPLVQEIV TADIGCAECQKKLANILSKM+DTE
Sbjct: 10  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFTADIGCAECQKKLANILSKMNDTE 69

Query: 63  SVVVNLLDKKVILTRRSQIP-SRVSTIRRLFASPCR 98
           SVVVNLLDKKVILTR+ QIP SRVST++RL  S  R
Sbjct: 70  SVVVNLLDKKVILTRKLQIPSSRVSTVKRLLGSSFR 105

[Close]

BLAST of CSPI05G25150 vs. ExPASy TrEMBL
Match: A0A6J1G188 (uncharacterized protein LOC111449743 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449743 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.0e-29
Identity = 74/89 (83.15%), Postives = 81/89 (91.01%), Query Frame = 0


Query: 7   GIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTESVVV 66
           GIGVRK +IFPHASSLAS+ESL+LPLVQEIV  ADIGCAECQKKLANIL+KM+DTESVVV
Sbjct: 12  GIGVRKLKIFPHASSLASVESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTESVVV 71

Query: 67  NLLDKKVILTRRSQI-PSRVSTIRRLFAS 95
           NLLDKKVILTR+ QI  SRVSTI+RL  S
Sbjct: 72  NLLDKKVILTRKLQIQTSRVSTIKRLLGS 100

[Close]

BLAST of CSPI05G25150 vs. ExPASy TrEMBL
Match: A0A6J1DB71 (uncharacterized protein LOC111018707 OS=Momordica charantia OX=3673 GN=LOC111018707 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.1e-27
Identity = 73/97 (75.26%), Postives = 81/97 (83.51%), Query Frame = 0


Query: 8   IGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTESVVVN 67
           IGVRK RIFPHASSLASIESL+LPLVQEIVLTADI C ECQ KLANILSK++DTESVVVN
Sbjct: 12  IGVRKPRIFPHASSLASIESLSLPLVQEIVLTADIRCPECQNKLANILSKINDTESVVVN 71

Query: 68  LLDKKVILTRRSQIP-------SRVSTIRRLFASPCR 98
           LL+KKVILTRR Q+P       S+V+TI+RL  S  R
Sbjct: 72  LLEKKVILTRRLQVPSTCSNSSSKVATIKRLLGSSFR 108

[Close]

BLAST of CSPI05G25150 vs. ExPASy TrEMBL
Match: A0A6J1G176 (uncharacterized protein LOC111449743 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449743 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.6e-25
Identity = 74/116 (63.79%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 7   GIGVRKKRIFPHASSLASIESLTLPL---------------------------VQEIVLT 66
           GIGVRK +IFPHASSLAS+ESL+LPL                           VQEIV  
Sbjct: 12  GIGVRKLKIFPHASSLASVESLSLPLVSAFSLYLRRFSSSPAEINALWSVILQVQEIVFA 71

Query: 67  ADIGCAECQKKLANILSKMSDTESVVVNLLDKKVILTRRSQI-PSRVSTIRRLFAS 95
           ADIGCAECQKKLANIL+KM+DTESVVVNLLDKKVILTR+ QI  SRVSTI+RL  S
Sbjct: 72  ADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQIQTSRVSTIKRLLGS 127

[Close]

BLAST of CSPI05G25150 vs. NCBI nr
Match: KGN52069.1 (hypothetical protein Csa_009028 [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 2.9e-42
Identity = 96/97 (98.97%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSD 60
          MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKM+D
Sbjct: 1  MGAVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMND 60

Query: 61 TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR 98
          TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR
Sbjct: 61 TESVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR 97

[Close]

BLAST of CSPI05G25150 vs. NCBI nr
Match: XP_038892707.1 (uncharacterized protein LOC120081691 [Benincasa hispida])

HSP 1 Score: 162.5 bits (410), Expect = 1.8e-36
Identity = 84/95 (88.42%), Postives = 89/95 (93.68%), Query Frame = 0


Query: 3  AVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTE 62
          A   GIGVRKK+IFPHASSLASIESL+LPLVQEIVLTADIGCAECQKKLANILSKM+DTE
Sbjct: 2  AAAAGIGVRKKKIFPHASSLASIESLSLPLVQEIVLTADIGCAECQKKLANILSKMNDTE 61

Query: 63 SVVVNLLDKKVILTRRSQIPSRVSTIRRLFASPCR 98
          SVVVNLLDKKVILTRR QIPSR+STI+RL  SPCR
Sbjct: 62 SVVVNLLDKKVILTRRLQIPSRISTIKRLLGSPCR 96

[Close]

BLAST of CSPI05G25150 vs. NCBI nr
Match: XP_022968296.1 (uncharacterized protein LOC111467567 isoform X3 [Cucurbita maxima])

HSP 1 Score: 145.2 bits (365), Expect = 3.0e-31
Identity = 79/96 (82.29%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 3   AVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTE 62
           A   GIGVRK +IFPHASSLASIESL+LPLVQEIV TADIGCAECQKKLANILSKM+DTE
Sbjct: 10  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFTADIGCAECQKKLANILSKMNDTE 69

Query: 63  SVVVNLLDKKVILTRRSQIP-SRVSTIRRLFASPCR 98
           SVVVNLLDKKVILTR+ QIP SRVST++RL  S  R
Sbjct: 70  SVVVNLLDKKVILTRKLQIPSSRVSTVKRLLGSSFR 105

[Close]

BLAST of CSPI05G25150 vs. NCBI nr
Match: KAG7013033.1 (hypothetical protein SDJN02_25789 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 142.5 bits (358), Expect = 1.9e-30
Identity = 78/96 (81.25%), Postives = 84/96 (87.50%), Query Frame = 0


Query: 3   AVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTE 62
           A   GIGVRK +IFPHASSLASIESL+LPLVQEIV  ADIGCAECQKKLANIL+KM+DTE
Sbjct: 9   AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTE 68

Query: 63  SVVVNLLDKKVILTRRSQIP-SRVSTIRRLFASPCR 98
           SVVVNLLDKKVILTR+ QIP SRVSTI+RL  S  R
Sbjct: 69  SVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 104

[Close]

BLAST of CSPI05G25150 vs. NCBI nr
Match: XP_023542320.1 (uncharacterized protein LOC111802252 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.5 bits (358), Expect = 1.9e-30
Identity = 78/96 (81.25%), Postives = 84/96 (87.50%), Query Frame = 0


Query: 3   AVVVGIGVRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTE 62
           A   GIGVRK +IFPHASSLASIESL+LPLVQEIV  ADIGCAECQKKLANIL+KM+DTE
Sbjct: 10  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTE 69

Query: 63  SVVVNLLDKKVILTRRSQIP-SRVSTIRRLFASPCR 98
           SVVVNLLDKKVILTR+ QIP SRVSTI+RL  S  R
Sbjct: 70  SVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105

[Close]

BLAST of CSPI05G25150 vs. TAIR 10
Match: AT1G68585.1 (unknown protein; Has 23 Blast hits to 23 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 71.2 bits (173), Expect = 5.1e-13
Identity = 41/94 (43.62%), Postives = 66/94 (70.21%), Query Frame = 0


Query: 10  VRKKRIFPHASSLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTESVVVNLL 69
           +R K      +SLAS+ SL++PL+QEIVL+ADI C++CQ+K+A+I+++M +T S++V++L
Sbjct: 16  LRSKLSRTSETSLASVASLSMPLIQEIVLSADIRCSDCQEKVADIMARMIETYSILVSVL 75

Query: 70  DKKVILT------RR------SQIPSRVSTIRRL 92
           +KKV LT      RR        +  ++STI+RL
Sbjct: 76  EKKVTLTCTYSGDRRVSKSYGEALLCKISTIKRL 109

[Close]

BLAST of CSPI05G25150 vs. TAIR 10
Match: AT2G35730.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 6.0e-06
Identity = 27/67 (40.30%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 21 SLASIESLTLPLVQEIVLTADIGCAECQKKLANILSKMSDTESVVVNLLDKKVILTRRSQ 80
          +L   E L+LP  Q I + AD+GC  CQ +++ I+SKM+  E  VV+ L KK+++ R   
Sbjct: 10 TLMLCEKLSLPSFQVIEINADVGCVACQDRVSKIVSKMTGIEEYVVD-LKKKLVMARGDF 69

Query: 81 IPSRVST 88
           P  VS+
Sbjct: 70 RPRLVSS 75

The following BLAST results are available for this feature:

BLAST of CSPI05G25150 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI05G25150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT62	1.4e-42	98.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608570 PE=4 SV=1	[more]
A0A6J1HUG9	1.4e-31	82.29	uncharacterized protein LOC111467567 isoform X3 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1G188	3.0e-29	83.15	uncharacterized protein LOC111449743 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1DB71	2.1e-27	75.26	uncharacterized protein LOC111018707 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1G176	7.6e-25	63.79	uncharacterized protein LOC111449743 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of CSPI05G25150 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52069.1	2.9e-42	98.97	hypothetical protein Csa_009028 [Cucumis sativus]	[more]
XP_038892707.1	1.8e-36	88.42	uncharacterized protein LOC120081691 [Benincasa hispida]	[more]
XP_022968296.1	3.0e-31	82.29	uncharacterized protein LOC111467567 isoform X3 [Cucurbita maxima]	[more]
KAG7013033.1	1.9e-30	81.25	hypothetical protein SDJN02_25789 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023542320.1	1.9e-30	81.25	uncharacterized protein LOC111802252 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI05G25150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68585.1	5.1e-13	43.62	unknown protein; Has 23 Blast hits to 23 proteins in 6 species: Archae - 0; Bact...	[more]
AT2G35730.1	6.0e-06	40.30	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 32..91 e-value: 3.9E-5 score: 25.8
None	No IPR available	PANTHER	PTHR47294:SF4	PROTEIN, PUTATIVE-RELATED	coord: 15..85
None	No IPR available	PANTHER	PTHR47294	OS08G0431150 PROTEIN	coord: 15..85
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 34..83

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G25150.1	CSPI05G25150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding