CSPI05G24490 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G24490
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProlyl endopeptidase
LocationChr5: 24091624 .. 24096034 (+)
RNA-Seq ExpressionCSPI05G24490
SyntenyCSPI05G24490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTCAAAACTCTTCTAAAACCCAAATCCTCCATTACAAAATTCTTCCTTTCTTCTTTGTCATTCTCCTCCTTCTGCAAACAACAACAACAACCCATCTTCTCATTCCCTTCCCAGTCCCCACCTTCTCCTAAGAAACTCCCTTTCACTCACTCTGTTCATGGCGTTAAGCTGCAAGATCCCTACCACTGGATGTCCAATACCCACGACCCTGATTTCGCTGACTATCTCCGTCAAGAAAACCTGTATGCTGAAGCATTCATGGCTGACACTCAGGTTCTACAGCGGCAGCTCTTCTCCGAGATGACGAGTCGAATTCCCGCCAAGGTTTCCACTCCCCCTGAGCCTTGGGGACCCTGGTCTGCTCTTTTCTCCTACTCCTAATCTTCTACTTTTTAATTTCGTTGAACGTTGCTGAAGCTGAATTTATCGATGCATCTTATTAGGTTTTACTACCAGTACATTCCGGATGGGAAGGAGTACCCAGTTCTATGCCGTAGGTTACAGAATGAGAAAAGCAGTTGGTTTAGGAAAATTTTAAATTTTGGTAAAGGGAATTCTGGGAAGGAAGAACAAGTATTGCTTGATTGGAATGAAATTGCTAAACAATACGGTAAGTTTATTGAGAACTTTTCTATTTTATTTGTGTCTATGTTGAGCTAAAGTGTACGGAAAATCGTTCTTCATCATGTTCTATCTGAATTATGAACAGTGAAAGAGCTACAGATACTTTACTATTGTTTATATAAGTCGTAAATGCCATTTTCTCTTTTGGGTAATACGTCGTTGATGATTGGTTCACTGTGTTTATCAAGGAATTTTCATTTTCGATCTTACTTCCTGTTATGAGAATTACGGCATTTCATTAAATTTTACGTTGTGTTGCTCACAATTGTAGGCTATGTTCACGTGGGAACTTGTCGTATTTCACCAGACCATAACTTTCTAGCATATACAGTTGATATTACTGGCAATGAACACTTCATGCTTCAGATTAAAGACCTGAGAAATGGACTGATAATTCCCAAGTTACAGAAGGAGGGAGTTGTAAGTTTGGCTTGGGCGGAAGAAGGCAGGGTGCTTTTCTATACTCAAGCAGATGAGAATCAGCGACCTTACAGGCAAATTTTTGTCAACGTTTTTAGCTCTTTTTTCTTCTAATAAATACATATACCTGACATTTATTCTTTTTTGCTTCTGCCTTAACATTTGACAGGGTTTTCTGCACAAAAGTTGGATTTAACGACACAGAGGATGTCTCGGTGTTTGTTGAAAATGATCCCAACTATTGTGTAGACATAACAAGCACAAAAGATGGGAAGTTCATAACTGTGAATTCAAACTCGAGGACTTCGTCTGAGGAAGGAACTTACCTATTTCAATTTTTTTCTTCTTTTTCTATCTTTTTTCTCTTAAGGAGATTTTATTTAAGCCAACTTAAGACTTCTATTTTGAAGCTTTACTGGTTTGTTTTACCCAGGTTTATATCATAGATGCCAACAACTCATTAGGTGGATTGCAAAGAATACATGAACGCATTCCTGGTATTCAATACTTTTTGGAACATCATCATGGTTTCTTTTATATCCTAACAAATGCTCCTCTTGAAAAGAATGGGGGTTGCTCGGAAGAAGATTATTATGTAGCTCGATGTCGAGTTGAAGATATTAAGTCAGCAGATTGGCAGGTTACTTTTATGCTACATCTGTTCCACATCTGATCTCAGTGTCCCTTCTTTTATCAACTGCGTGCACGTGTGTTTGTTTTAATTTAGTTCTAATTTTTGAGTTACTTCCACTCAACTAATCTCCTTTTTCATTTTCCACATACCTTGTCATGTCATGTGTGTATTATAGGATATCGTCCTTCAGAGTGAAGATTTCAGCATACAGGACATGGATATTTTCAGTGGACATCTTGTGCTTTTTGTCAATAAGAATGGTGTTTCTATGTTATGTTCAATCAATTTTCCTTTAGATGCTGATCATAACGTATGGGTTTGTTTTTCTTGGCTTGCTTTATGGTTAAATTAGCATCAGTATAACAATTCCTGTATATCATCTCATTCTACTTGTTCTGCAGCATCACTTGGAGATTGACAAACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAGCGTAGCTCCAGGATCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTCTCATCACCAGTGGTATTACTTTGTAATTTTATATATCTGCATGTGCATGAAATATCTTGGCACGTACTTATATCTGATTTTGTCTATTGAGTGGTTGTACTTTCTTCTCCCTTATTTTTGGTTTCCTTTGAATTATGAAGCTAAGTTTTATCTAGTAAATCATGATAAATTTATGAAATTATTTGCTTCTTATGCCTCTTGCTATAAAGCAGCTAAAAATTATTTTTCCTTATAGTTTTTGATAAAATTACACATTTGTGAGCATTATTGTCTTCAATGTGCTAGCATACACTGTCATAATTGGTACTTAGAATAGAGTTTTAAACTTAAATTTACCCCACTCTTCTGGCTTCATTTTGAAGTGTGAACCTTTCTAAAAATTTCATGTTTTCTTGGAACCACCAGATGCCAGATTTGATTGTTGACTATGACATGTCCAAACGGATCTTTTCAATCATTCAGCAAGAGGAAGTAAAAGCCCAGCATGATGTTGAACTTAAAACAAATCTACCAGATACGTTGGATGCTGAGGAAGTTTCAGACACACAAAGCAAAAGAGAAAATTTCCAGAATTGTGAATCCCAAAATTGGAAGGACTTTTCGGAGGCATACTTTTGCGAAAGGATAGAAGTTAAATCACATGACGGCATCAGAATACCCTTGACCATATTGTATTCCCCAATGACTTTTCAAAAAGGACAGTCACCTGGAATTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGCTGGTGTCCTTATCGCCTGAGTTTACTTGACCGTGGTTTTGTGCTGGCGTTTGCAGATGTCAGGTAGGTTCTATCGTTATATAATGTGTGTATGTGTGTGTTTGTATTGTTTCAATCTCTGTTAATTGAAACTCAATTTAGAACCTTGATTGTGCCTATGAGAATTTTAATTTTTACATGACATGGATTTTGGTGGGCAGGGGAGGAGGTGGTGGTGGTGATTCTTCATGGCATAGATGTGGGAGTGGGCTTGAGAAACCGAACTCAATACACGACTTCATCTCTTGTGCAAATTTTCTCATTCATAATGGTTATGTTCATAAGGATCGACTGGGTTCCATTGGATACAGTGCTGGAGGTCTTCTAGTTGGGGCTGCAATCAACATGCATCCCAATCTGTTTTGCGCAGCCATTTTAAAAGTTAGTTTGAGCCATACACTTGCCTCCTCATTGGTCTTCAAATCTTGTAAAGTAGATTGGAAATGCATCTAGTTTTATTTACTTTATTCATAAAAGGTTTTTATATGGCAATTCAATTTCAGTCCTGTTCTTTTACTTTCTAATTTCAAGTTAAGGTTCCTATTAAATTAGTTAAAGGGATGTTTCACCATCGGTAATGAACCCAGAGCAAAGGAAGATATTCCTAGAAACGTTGGAGATATTGAAGAATTTTAAATAATGAACTGATTCAACTAGACCAGAAGCCCGATAACTTTGTGTGAGGTTGAATTGGTGTTATATTCAGGATTGAAGCAATTGTACATTATAATTAATTAGGTAGGTAAAATTGATCCTATCAATACACACAGTGGAAGAAAATAGACTAACAATAAGAAGGAAATGTACAAGAAGAGAAACTGTGACTTCCAAAGTTTTAATGTTCTGCTTGGTTACTCTCAGATTTCTTTTTTATGGGGGTTGAAGGTTTTCTATGTTTAATCAGGTTCCATTTCTCGACATATGTAACACCCTACTGGATCCCAGTTTACCTCTCACCGTTCTGGATTATGAAGAATTTGGAAACCCACAAATACAAAAGCAATTTGAGTCTATTTTAAGCTATTCTCCTTACGATAATATATCCAAGGGAACCTGTTATCCTTCAATGCTCGTCACAGCGTCATTCCATGATGCAAGGTAAATTATTTTTTTCTTTCAACTGTATCACATTAGCATATTTGTCTGAACAGGTAAATGTTCTCTCGTGCTTTGCTCTCAAATAAACATATGTGAAATCATTGAATTTCATCCTCAGGGTTGGAGTATGGGAAGCTGCTAAGTGGGTGGCAAAAATTCGAGACACTACGTGCTCTCATTGTTCAACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTCGGTGAAGGTGGTCTCTATGGTGGATGTGAAGAGACGGCTTATGAGTACGCATTTCTCATCAAAGTCCTCAGAACTCCTGACCACGATTAA

mRNA sequence

ATGGCTCTCAAAACTCTTCTAAAACCCAAATCCTCCATTACAAAATTCTTCCTTTCTTCTTTGTCATTCTCCTCCTTCTGCAAACAACAACAACAACCCATCTTCTCATTCCCTTCCCAGTCCCCACCTTCTCCTAAGAAACTCCCTTTCACTCACTCTGTTCATGGCGTTAAGCTGCAAGATCCCTACCACTGGATGTCCAATACCCACGACCCTGATTTCGCTGACTATCTCCGTCAAGAAAACCTGTATGCTGAAGCATTCATGGCTGACACTCAGGTTCTACAGCGGCAGCTCTTCTCCGAGATGACGAGTCGAATTCCCGCCAAGGTTTCCACTCCCCCTGAGCCTTGGGGACCCTGGTTTTACTACCAGTACATTCCGGATGGGAAGGAGTACCCAGTTCTATGCCGTAGGTTACAGAATGAGAAAAGCAGTTGGTTTAGGAAAATTTTAAATTTTGGTAAAGGGAATTCTGGGAAGGAAGAACAAGTATTGCTTGATTGGAATGAAATTGCTAAACAATACGGCTATGTTCACGTGGGAACTTGTCGTATTTCACCAGACCATAACTTTCTAGCATATACAGTTGATATTACTGGCAATGAACACTTCATGCTTCAGATTAAAGACCTGAGAAATGGACTGATAATTCCCAAGTTACAGAAGGAGGGAGTTGTAAGTTTGGCTTGGGCGGAAGAAGGCAGGGTGCTTTTCTATACTCAAGCAGATGAGAATCAGCGACCTTACAGGGTTTTCTGCACAAAAGTTGGATTTAACGACACAGAGGATGTCTCGGTGTTTGTTGAAAATGATCCCAACTATTGTGTAGACATAACAAGCACAAAAGATGGGAAGTTCATAACTGTTTATATCATAGATGCCAACAACTCATTAGGTGGATTGCAAAGAATACATGAACGCATTCCTGGTATTCAATACTTTTTGGAACATCATCATGGTTTCTTTTATATCCTAACAAATGCTCCTCTTGAAAAGAATGGGGGTTGCTCGGAAGAAGATTATTATGTAGCTCGATGTCGAGTTGAAGATATTAAGTCAGCAGATTGGCAGGATATCGTCCTTCAGAGTGAAGATTTCAGCATACAGGACATGGATATTTTCAGTGGACATCTTGTGCTTTTTGTCAATAAGAATGGTGTTTCTATGTTATGTTCAATCAATTTTCCTTTAGATGCTGATCATAACCATCACTTGGAGATTGACAAACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAGCGTAGCTCCAGGATCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTCTCATCACCAGTGATGCCAGATTTGATTGTTGACTATGACATGTCCAAACGGATCTTTTCAATCATTCAGCAAGAGGAAGTAAAAGCCCAGCATGATGTTGAACTTAAAACAAATCTACCAGATACGTTGGATGCTGAGGAAGTTTCAGACACACAAAGCAAAAGAGAAAATTTCCAGAATTGTGAATCCCAAAATTGGAAGGACTTTTCGGAGGCATACTTTTGCGAAAGGATAGAAGTTAAATCACATGACGGCATCAGAATACCCTTGACCATATTGTATTCCCCAATGACTTTTCAAAAAGGACAGTCACCTGGAATTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGCTGGTGTCCTTATCGCCTGAGTTTACTTGACCGTGGTTTTGTGCTGGCGTTTGCAGATGTCAGGGGAGGAGGTGGTGGTGGTGATTCTTCATGGCATAGATGTGGGAGTGGGCTTGAGAAACCGAACTCAATACACGACTTCATCTCTTGTGCAAATTTTCTCATTCATAATGGTTATGTTCATAAGGATCGACTGGGTTCCATTGGATACAGTGCTGGAGGTCTTCTAGTTGGGGCTGCAATCAACATGCATCCCAATCTGTTTTGCGCAGCCATTTTAAAAGTTCCATTTCTCGACATATGTAACACCCTACTGGATCCCAGTTTACCTCTCACCGTTCTGGATTATGAAGAATTTGGAAACCCACAAATACAAAAGCAATTTGAGTCTATTTTAAGCTATTCTCCTTACGATAATATATCCAAGGGAACCTGTTATCCTTCAATGCTCGTCACAGCGTCATTCCATGATGCAAGGGTTGGAGTATGGGAAGCTGCTAAGTGGGTGGCAAAAATTCGAGACACTACGTGCTCTCATTGTTCAACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTCGGTGAAGGTGGTCTCTATGGTGGATGTGAAGAGACGGCTTATGAGTACGCATTTCTCATCAAAGTCCTCAGAACTCCTGACCACGATTAA

Coding sequence (CDS)

ATGGCTCTCAAAACTCTTCTAAAACCCAAATCCTCCATTACAAAATTCTTCCTTTCTTCTTTGTCATTCTCCTCCTTCTGCAAACAACAACAACAACCCATCTTCTCATTCCCTTCCCAGTCCCCACCTTCTCCTAAGAAACTCCCTTTCACTCACTCTGTTCATGGCGTTAAGCTGCAAGATCCCTACCACTGGATGTCCAATACCCACGACCCTGATTTCGCTGACTATCTCCGTCAAGAAAACCTGTATGCTGAAGCATTCATGGCTGACACTCAGGTTCTACAGCGGCAGCTCTTCTCCGAGATGACGAGTCGAATTCCCGCCAAGGTTTCCACTCCCCCTGAGCCTTGGGGACCCTGGTTTTACTACCAGTACATTCCGGATGGGAAGGAGTACCCAGTTCTATGCCGTAGGTTACAGAATGAGAAAAGCAGTTGGTTTAGGAAAATTTTAAATTTTGGTAAAGGGAATTCTGGGAAGGAAGAACAAGTATTGCTTGATTGGAATGAAATTGCTAAACAATACGGCTATGTTCACGTGGGAACTTGTCGTATTTCACCAGACCATAACTTTCTAGCATATACAGTTGATATTACTGGCAATGAACACTTCATGCTTCAGATTAAAGACCTGAGAAATGGACTGATAATTCCCAAGTTACAGAAGGAGGGAGTTGTAAGTTTGGCTTGGGCGGAAGAAGGCAGGGTGCTTTTCTATACTCAAGCAGATGAGAATCAGCGACCTTACAGGGTTTTCTGCACAAAAGTTGGATTTAACGACACAGAGGATGTCTCGGTGTTTGTTGAAAATGATCCCAACTATTGTGTAGACATAACAAGCACAAAAGATGGGAAGTTCATAACTGTTTATATCATAGATGCCAACAACTCATTAGGTGGATTGCAAAGAATACATGAACGCATTCCTGGTATTCAATACTTTTTGGAACATCATCATGGTTTCTTTTATATCCTAACAAATGCTCCTCTTGAAAAGAATGGGGGTTGCTCGGAAGAAGATTATTATGTAGCTCGATGTCGAGTTGAAGATATTAAGTCAGCAGATTGGCAGGATATCGTCCTTCAGAGTGAAGATTTCAGCATACAGGACATGGATATTTTCAGTGGACATCTTGTGCTTTTTGTCAATAAGAATGGTGTTTCTATGTTATGTTCAATCAATTTTCCTTTAGATGCTGATCATAACCATCACTTGGAGATTGACAAACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAGCGTAGCTCCAGGATCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTCTCATCACCAGTGATGCCAGATTTGATTGTTGACTATGACATGTCCAAACGGATCTTTTCAATCATTCAGCAAGAGGAAGTAAAAGCCCAGCATGATGTTGAACTTAAAACAAATCTACCAGATACGTTGGATGCTGAGGAAGTTTCAGACACACAAAGCAAAAGAGAAAATTTCCAGAATTGTGAATCCCAAAATTGGAAGGACTTTTCGGAGGCATACTTTTGCGAAAGGATAGAAGTTAAATCACATGACGGCATCAGAATACCCTTGACCATATTGTATTCCCCAATGACTTTTCAAAAAGGACAGTCACCTGGAATTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGCTGGTGTCCTTATCGCCTGAGTTTACTTGACCGTGGTTTTGTGCTGGCGTTTGCAGATGTCAGGGGAGGAGGTGGTGGTGGTGATTCTTCATGGCATAGATGTGGGAGTGGGCTTGAGAAACCGAACTCAATACACGACTTCATCTCTTGTGCAAATTTTCTCATTCATAATGGTTATGTTCATAAGGATCGACTGGGTTCCATTGGATACAGTGCTGGAGGTCTTCTAGTTGGGGCTGCAATCAACATGCATCCCAATCTGTTTTGCGCAGCCATTTTAAAAGTTCCATTTCTCGACATATGTAACACCCTACTGGATCCCAGTTTACCTCTCACCGTTCTGGATTATGAAGAATTTGGAAACCCACAAATACAAAAGCAATTTGAGTCTATTTTAAGCTATTCTCCTTACGATAATATATCCAAGGGAACCTGTTATCCTTCAATGCTCGTCACAGCGTCATTCCATGATGCAAGGGTTGGAGTATGGGAAGCTGCTAAGTGGGTGGCAAAAATTCGAGACACTACGTGCTCTCATTGTTCAACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTCGGTGAAGGTGGTCTCTATGGTGGATGTGAAGAGACGGCTTATGAGTACGCATTTCTCATCAAAGTCCTCAGAACTCCTGACCACGATTAA

Protein sequence

MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLRTPDHD*
Homology
BLAST of CSPI05G24490 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.8e-96
Identity = 231/753 (30.68%), Postives = 363/753 (48.21%), Query Frame = 0

Query: 43  PSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSE 102
           P  K++P  H +HG   +D Y+W+ +  + +   YL +EN Y    M   Q    Q++  
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKDRDNTEVIQYLEEENRYYHEIMRPLQEQTEQIYES 63

Query: 103 MTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKE 162
           M  R+P      P   G +FYY  +   K+YP+  R     K +  R +L          
Sbjct: 64  MVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYAR-----KQAASRALL------QDAT 123

Query: 163 EQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLI----I 222
           E+V+LD NE+A++  Y+ V   R++ DH+ LAY  +  G + + + IKDL  G +    +
Sbjct: 124 EEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLSDRV 183

Query: 223 PKLQKEGVVSLAWAEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVD 282
           P +   G  S+ W   G  +FYT  DE+QRP +++  ++G +   D  +F E D  + + 
Sbjct: 184 PNVYIYG--SMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTLF 243

Query: 283 ITSTKDGKFITVY----------IIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTN 342
           I+ ++ GKFI VY          +ID ++ L  LQ + ER  GI Y +EH      ILTN
Sbjct: 244 ISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLILTN 303

Query: 343 APLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGV 402
                       ++ + RC + D+ S    ++V  +E+  +Q+M  F   L++   +NG+
Sbjct: 304 E--------GALNFQLLRCPLNDLSSK--VNVVEYNEERYLQEMYPFRDKLLIAGRENGL 363

Query: 403 SMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMP 462
           + +  +         H  E+ ++  W  P                   LY V + S    
Sbjct: 364 TQIWVV---------HDGELQQIS-WDEP-------------------LYTVAVLSE--- 423

Query: 463 DLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNW 522
                YD ++ +         K    + L+T     L    VS  +  R  F+       
Sbjct: 424 ---QSYDTNEVLIQYESLLTPKTTFGLNLQTGEKQCLQVAPVSG-EYDRSQFRQ------ 483

Query: 523 KDFSEAYFCERIEVKSHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYR 582
                    E++      G+++P+T +Y       G +P IL GYG+YG   D  + PYR
Sbjct: 484 ---------EQLWATGRSGVKVPMTAVYLEGALDNGPAPLILYGYGSYGSNSDPRFDPYR 543

Query: 583 LSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRL 642
           L LL++G V   A VRGG   G   W+  G    K N+  DFI+ A  LI   Y    ++
Sbjct: 544 LPLLEKGIVFVTAQVRGGSEMG-RGWYEDGKMQNKRNTFTDFIAAAKHLIDQNYTSPTKM 603

Query: 643 GSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQ 702
            + G SAGGLLVGA  NM   LF   +  VPF+D+  T+LD S+PLT L+++E+G+P+ Q
Sbjct: 604 AARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTLEWDEWGDPRKQ 663

Query: 703 KQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAI 762
           + +  + SYSPYDN+ +   YP M +T   +D RVG +E AKWVA++R       + + +
Sbjct: 664 EDYFYMKSYSPYDNV-EAKDYPHMYITTGINDPRVGYFEPAKWVARLRAVKTD--NNTLV 678

Query: 763 LKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 782
           +KTNM  GHFG+ G +   +E A  YAF++  L
Sbjct: 724 MKTNMGAGHFGKSGRFNHLKEAAESYAFILDKL 678

BLAST of CSPI05G24490 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 3.0e-84
Identity = 212/752 (28.19%), Postives = 352/752 (46.81%), Query Frame = 0

Query: 43  PSPKKLPFTHSVHGVKLQDPYHWM--SNTHDPDFADYLRQENLYAEAFMADTQVLQRQLF 102
           P   ++P   ++HG    D Y+W+       P+  DYL+QEN Y    MA  Q LQ ++ 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 103 SEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRR--LQNEKSSWFRKILNFGKGN 162
            E+  RIP +  + P     + Y      G EY +  R+     E   W           
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEW----------- 122

Query: 163 SGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLII 222
                + LLD N+ A    +  +G   I+PD+  +A   D      + ++ ++L  G   
Sbjct: 123 -----ETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWY 182

Query: 223 PKLQKEGVVSLAWAEEGRVLFYTQADE-NQRPYRVFCTKVGFNDTEDVSVFVENDPNYCV 282
           P+L      S  WA +  + +Y +       PY+V+   +G   ++D  ++ E D  Y V
Sbjct: 183 PELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYV 242

Query: 283 DITSTKDGKFITVYIIDANNSLGGL--QRIHERIPGI--------QYFLEHHHGFFYILT 342
            +  T    ++ +++  A  S   L    + +  P +        +Y L+H+   FY+ +
Sbjct: 243 SLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRS 302

Query: 343 NAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNG 402
           N    ++G    +++ + R R+ D     W++++   E+  ++   +F+  LV+   + G
Sbjct: 303 N----RHG----KNFGLYRTRMRD--EQQWEELIPPRENIMLEGFTLFTDWLVVEERQRG 362

Query: 403 VSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVM 462
           ++ L  IN           +  ++    F  P+    +A   N +  ++  R   SS   
Sbjct: 363 LTSLRQIN----------RKTREVIGIAFDDPAYVTWIA--YNPEPETARLRYGYSSMTT 422

Query: 463 PDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQN 522
           PD + + DM      +++Q EV                                      
Sbjct: 423 PDTLFELDMDTGERRVLKQTEVPG------------------------------------ 482

Query: 523 WKDFSEAYFCERIEVKSHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPY 582
              ++  Y  E + + + DG+ +P++++Y    F+KG +P ++ GYG+YG  +D  +   
Sbjct: 483 --FYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFS 542

Query: 583 RLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDR 642
           RLSLLDRGFV A   VRGGG  G   W+  G  L+K N+ +D++   + L+  GY     
Sbjct: 543 RLSLLDRGFVYAIVHVRGGGELG-QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSL 602

Query: 643 LGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQI 702
             ++G SAGG+L+G AIN  P LF   I +VPF+D+  T+LD S+PLT  ++EE+GNPQ 
Sbjct: 603 CYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQD 662

Query: 703 QKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRD-TTCSHCSTS 762
            + +E + SYSPYDN++    YP +LVT   HD++V  WE AKWVAK+R+  T  H    
Sbjct: 663 PQYYEYMKSYSPYDNVT-AQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDHL--- 673

Query: 763 AILKTNMLGGHFGEGGLYGGCEETAYEYAFLI 779
            +L T+M  GH G+ G +   E  A EYAFL+
Sbjct: 723 LLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLV 673

BLAST of CSPI05G24490 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.6e-84
Identity = 216/760 (28.42%), Postives = 351/760 (46.18%), Query Frame = 0

Query: 39  SQSPPSPKKLP-FTHSVHGVKLQDPYHWM--SNTHDPDFADYLRQENLYAEAFMADTQVL 98
           S +PP   K P    + HG +  D Y+W+      + +   YL  EN Y +A MA  + L
Sbjct: 25  SATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYTDAVMAPLKPL 84

Query: 99  QRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFG 158
           + +L+ E+ +RI    ++ P     W+YY     GK+YPV  RR    K       ++  
Sbjct: 85  EDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARR----KDGPGVDAVSIQ 144

Query: 159 KGNSGKE---EQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDL 218
             N+  +   EQVLLD N +     Y +VG   +S D+  LAY  D  G   + ++ K+L
Sbjct: 145 AANAAGDFAGEQVLLDVNALGAGKDYYNVGDYEVSQDNRLLAYADDTNGRRQYTIRFKNL 204

Query: 219 RNGLIIPKLQKEGVVSLAWAEEGRVLFYTQAD-ENQRPYRVFCTKVGFNDTEDVSVFVEN 278
             G ++P        +L W+++GR LFY   D E     RV    +G   ++D  V+ E 
Sbjct: 205 DTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQDALVYEEE 264

Query: 279 DPNYCVDITSTKDGKFITVYIIDANN---------SLGGLQRIHERIPGIQYFLEHHHGF 338
           D ++ + I  ++D KFI + +    +         S G    +  R   ++Y  +H    
Sbjct: 265 DDSFYMGIGRSRDDKFICISVESTVSSEMRCTPAASPGVFTVLAPRERDVEYQADHLGDR 324

Query: 339 FYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLF 398
           + I TNA    N       + +     +     DW+D V   +D  ++  ++F G  V+ 
Sbjct: 325 WVIRTNADGATN-------FKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGFSVVA 384

Query: 399 VNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVL 458
              N +  L  I     +D+       K D   +   S   S  P +  D++   Y   +
Sbjct: 385 ERANALESLRVIKADGSSDY------VKADESAY---SMGLSANPETGTDWLRYSY-TSM 444

Query: 459 SSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQN 518
           ++P                           +++  KT     L  + V    + +     
Sbjct: 445 TTPA------------------------TTYEINTKTGERRQLKQQPVPGYDASK----- 504

Query: 519 CESQNWKDFSEAYFCERIEVKSHDG-IRIPLTILYSPMTFQKGQSPGILQGYGAYGEILD 578
                       Y  ER+   + DG  +IP+T++Y     + G++P +   YG+YG  +D
Sbjct: 505 ------------YVTERVWAPARDGKTKIPVTLVYRKDVARDGKAPMLQYAYGSYGASMD 564

Query: 579 KSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNG 638
            ++    +SLLDRG V A A +RGG   G  +W+  G    K N+  DFI   ++L+  G
Sbjct: 565 PNFSITNVSLLDRGVVYALAHIRGGQEMG-RAWYDDGKLYNKINTFTDFIDVTDYLVKEG 624

Query: 639 YVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEE 698
           Y  KDR+ ++G SAGGLL+GA  NM P  +   +  VPF+D+  T+LDP++PLT  +Y+E
Sbjct: 625 YAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVDVVTTMLDPTIPLTTNEYDE 684

Query: 699 FGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCS 758
           +GNP+ +  ++ IL+YSPYDN+ +   YP+M V     D++V  WE AK+VA++RD    
Sbjct: 685 WGNPEEKGYYDYILTYSPYDNL-QAKAYPAMFVGTGLWDSQVQYWEPAKYVARLRDLNTG 718

Query: 759 HCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 782
                 + +TNM  GH G+ G +    E A  +AF++  L
Sbjct: 745 --KGPVVFRTNMEAGHGGKSGRFRQYRERAEMFAFMLDQL 718

BLAST of CSPI05G24490 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 2.0e-56
Identity = 191/775 (24.65%), Postives = 331/775 (42.71%), Query Frame = 0

Query: 39  SQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQ 98
           S  PP P+       +H     D Y W+ +  DPD   YL  EN YA+   +    L+  
Sbjct: 5   SLQPPLPRSERRIRVLHNDVTIDSYGWLRDREDPDVLAYLEAENHYADEVTSYVAELKAD 64

Query: 99  LFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGN 158
           L +E+  R     + PP   G +FY+Q    G  +           S+W+R+ +      
Sbjct: 65  LIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSGLLH-----------SAWWRRPV------ 124

Query: 159 SGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLII 218
           +G  E+++ D N +     +  +G    S D  ++A++ D+ GNE + L+++D+ NG  I
Sbjct: 125 TGGPEELVFDPNTLPGAEVFYSLGALEPSDDGRYIAFSFDLIGNERYELRVRDMTNGREI 184

Query: 219 PKLQKEGVVSLAWAEEGRVLFYTQADENQRPY-RVFCTKVGFNDTEDVSVFVENDPNYCV 278
            +        L WA + R LF+T+   ++R + RV    V    +E   VF E +    +
Sbjct: 185 WR-DPSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETGRSE--VVFEEVNERLAL 244

Query: 279 DITSTKDGKFITVYIIDANNSLGGLQ-------------------RIHERIPGIQYFLEH 338
            +  +  G ++ + +I  ++    +Q                   RI  R  G + + EH
Sbjct: 245 VVRRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWRRILARELGHEIYAEH 304

Query: 339 HHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGH 398
               F    N         +  +  + R  ++D   + WQ++V      +++++ +   H
Sbjct: 305 WGNEFLFRVND--------TGPNLRLVRTAIDDTSPSRWQEVVPHRAGITLEEIHVLEEH 364

Query: 399 LVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPG---------S 458
            V+ + + G+        P    H+ +    ++ P   P+  +SC+V  G         +
Sbjct: 365 -VIVLEREGIQ-------PRLVAHHRN---GRVGPSIVPV-EHSCTVTVGLSAGGSYSCA 424

Query: 459 NHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEE 518
            H +  S     + S V PD+ +                   QHD  L T+    L    
Sbjct: 425 RHPYRVSALTYKICSFVTPDIFI-------------------QHD--LLTDKSKVLYRTL 484

Query: 519 VSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMTFQKGQSPGI 578
           VS  +                  E Y    +  K+ DG+ +P++I+      + G  P +
Sbjct: 485 VSGFE-----------------PELYEARVVMAKAEDGVEVPISIVARRDRGEDG--PVL 544

Query: 579 LQGYGAYGEILDKSWCPY------RLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEK 638
           L  YG YG     ++  +      RLSLLDRG       VRGGG  G  +WH   +  +K
Sbjct: 545 LNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGGELG-RAWHEAATRDQK 604

Query: 639 PNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDI 698
             +  D I+ A  L+ + +  +D +   G SAGG  V AA  + P+LF A + +VP  DI
Sbjct: 605 RLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLRPDLFRAVLAEVPLADI 664

Query: 699 CNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARV 758
            +T LD +LP  + +  E+G+P +   ++ + SY PY N++    YP   + A+ HD++V
Sbjct: 665 IDTELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYNLTPDRRYPPTYIDAALHDSQV 697

Query: 759 GVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLI 779
             ++ A++VA+ R           I +T M+GGH G     G  EE A+  A+++
Sbjct: 725 LYYQPARYVAQRRSKAVDR-DPDLIFRTRMIGGHMGVSHGPGVAEEAAFRMAWIL 697

BLAST of CSPI05G24490 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 7.6e-56
Identity = 192/792 (24.24%), Postives = 333/792 (42.05%), Query Frame = 0

Query: 27  CKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDFADYLRQENLYAE 86
           C   +  +    S  PP P+  P    +H     D Y W+ +  +PD   YL  EN YAE
Sbjct: 24  CSHFRSVVMWDKSLRPPLPRAEPRIRVLHDDVTVDRYGWLRDRENPDVRAYLEAENSYAE 83

Query: 87  AFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSS 146
              A  + L+ +L +E+  R P + +TPP   GP+ Y+Q    G  +PV           
Sbjct: 84  QATAHLRRLKTELIAEIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPV----------- 143

Query: 147 WFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFM 206
           W+R+ +      +G   +++LD N I     +  +G    S D  +LA++VD+ G E + 
Sbjct: 144 WWRRPV------TGGSAELVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIGAERYE 203

Query: 207 LQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFYTQADENQRP-YRVFCTKVGFNDTEDV 266
           L+++D+ +G  + +     V  + WA +   LF+T+   ++R  +++    VG  ++E  
Sbjct: 204 LRVRDMSDGRDVWR-DAGSVGQVVWAADNHTLFFTRERPDRRQHHQIVRLNVGRGNSE-- 263

Query: 267 SVFVENDPNYCVDITSTKDGKFI-------------------TVYIIDANNSLGGLQRIH 326
            VF E +    V +  ++ G ++                    V+ + A+   G  +RI 
Sbjct: 264 VVFEEANERLAVLVRRSQSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGGQWRRIV 323

Query: 327 ERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSED 386
            R  G Q + EH +  F    +         +   + +    ++D   + W+++V     
Sbjct: 324 MRELGHQIYAEHWYDRFLFRVDD--------AGPYWRLVSAPIDDPSPSRWEEVVPHRAG 383

Query: 387 FSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVA 446
            +I ++ +   HLVL   +     L S N              ++     P    SC++ 
Sbjct: 384 VTIDEIHVLEQHLVLLEREGLRPRLISRN-----------RSGRVGAVIVP-DEPSCTIR 443

Query: 447 PG---------SNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVEL 506
            G         + H F SS     +SS V PD  +++D +     ++             
Sbjct: 444 VGLSAGGCYSAARHPFRSSKLTYSVSSFVTPDTFIEHDFANDRSVVL------------- 503

Query: 507 KTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYS 566
                                    CE++     +  Y    +  ++ DG+++P++++  
Sbjct: 504 -------------------------CEARVPGYDATQYLATVVMAEAEDGVQVPISLVAR 563

Query: 567 PMTFQKGQSPGILQGYGAYGEILDKSWCPY------RLSLLDRGFVLAFADVRGGGGGGD 626
                 G  P +L  YG YG     S+  +      RLSLLDR        VRGGG  G 
Sbjct: 564 RDRTSPG--PVLLSVYGCYGIPRLPSFLAWPSSMTARLSLLDREVAFGIVHVRGGGELG- 623

Query: 627 SSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF 686
             WH   +  +K  +  D IS    LI  G+  +D +   G S GG  V A     PNLF
Sbjct: 624 RPWHDAATRDQKRITHTDLISATEGLIERGFATRDGVVIEGKSGGGGTVLATAVFRPNLF 683

Query: 687 CAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPS 746
            A + +VP  DI +T LD ++P T+ +  E+G+PQ   ++  + SY PY N+S     P 
Sbjct: 684 RAVVAEVPLADIIDTQLDSTMPYTLKETAEYGDPQDAYEYRYLRSYDPYYNLSPERSLPP 733

Query: 747 MLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETA 784
             V A+  D +V  ++ A++VA+ R +  +      + +  M+GGH G     G  E+ A
Sbjct: 744 TYVDAALDDGQVIYYQPARYVAQ-RRSCATDRDPDLVFRIRMVGGHSGPSHGPGIAEQAA 733

BLAST of CSPI05G24490 vs. ExPASy TrEMBL
Match: A0A0A0KQZ9 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G606880 PE=3 SV=1)

HSP 1 Score: 1630.5 bits (4221), Expect = 0.0e+00
Identity = 782/787 (99.36%), Postives = 783/787 (99.49%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ
Sbjct: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP
Sbjct: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH
Sbjct: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY
Sbjct: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300
           TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG
Sbjct: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300

Query: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360
           GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI
Sbjct: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360

Query: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420
           VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS
Sbjct: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420

Query: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTN 480
           NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK QHDVELKTN
Sbjct: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKVQHDVELKTN 480

Query: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540
           LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT
Sbjct: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540

Query: 541 FQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600
           F+KGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG
Sbjct: 541 FKKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600

Query: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPF 660
           LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF AAILKVPF
Sbjct: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFRAAILKVPF 660

Query: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720
           LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD
Sbjct: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720

Query: 721 ARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKV 780
           ARVGVWEAAKWVAKIRDTTCS CSTSAILKTNMLGGHFGEGGLYGGCEE AYEYAFLIKV
Sbjct: 721 ARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEEMAYEYAFLIKV 780

Query: 781 LRTPDHD 788
           LRTPDHD
Sbjct: 781 LRTPDHD 787

BLAST of CSPI05G24490 vs. ExPASy TrEMBL
Match: A0A1S3BF53 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103489202 PE=3 SV=1)

HSP 1 Score: 1545.0 bits (3999), Expect = 0.0e+00
Identity = 741/797 (92.97%), Postives = 764/797 (95.86%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALK+LLKPKSSITKFFLSSLSFSSFCK QQQPIFSFP QSPPSPKKLPFTHSVHGV LQ
Sbjct: 13  MALKSLLKPKSSITKFFLSSLSFSSFCK-QQQPIFSFPPQSPPSPKKLPFTHSVHGVTLQ 72

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPD +DYLRQENLYAEAFMADT+VLQRQLFSEMT RIP+KVSTPPEPWGP
Sbjct: 73  DPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPEPWGP 132

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWF+KIL FGKGN GKEEQVLLDWNEIAK+YGYVH
Sbjct: 133 WFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRYGYVH 192

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGR+LFY
Sbjct: 193 VGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRMLFY 252

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT----------V 300
           TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKF+T          V
Sbjct: 253 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFVTVNSNSRTSSEV 312

Query: 301 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE 360
           YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKN  C EEDYYVARCRVE
Sbjct: 313 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDYYVARCRVE 372

Query: 361 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK 420
           DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSIN PLDAD NHHLEI+K
Sbjct: 373 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADDNHHLEIEK 432

Query: 421 LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK 480
           LDPWFFPLPSNSCSVAPGSNHDFMSS YRVVLSSPVMPDLIVDYDMSKR FSIIQQEEVK
Sbjct: 433 LDPWFFPLPSNSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFSIIQQEEVK 492

Query: 481 AQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRI 540
            QHDVELKTNLPDTLD +EVSDTQ+KRENFQNC+SQNWKDFSEAY CERIEV SHDG+ I
Sbjct: 493 VQHDVELKTNLPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEVTSHDGVGI 552

Query: 541 PLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 600
           PLTILY+PMTFQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG
Sbjct: 553 PLTILYTPMTFQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 612

Query: 601 DSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 660
           DSSWHR G+GLEKPNSIHDF+SCANFLI+NGYVHKDRLGSIGYSAGGLLVGAAINMHPNL
Sbjct: 613 DSSWHRSGNGLEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 672

Query: 661 FCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYP 720
           F AAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPY+NISKG+CYP
Sbjct: 673 FRAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYP 732

Query: 721 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEET 780
           SMLVTASFHDARVGVWEAAKWVAKIRDTTCS CS+SAILKTNMLGGHFGEGGLYGGCEE 
Sbjct: 733 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGGLYGGCEEM 792

Query: 781 AYEYAFLIKVLRTPDHD 788
           AYEYAFLIKVLRTPDHD
Sbjct: 793 AYEYAFLIKVLRTPDHD 808

BLAST of CSPI05G24490 vs. ExPASy TrEMBL
Match: A0A5A7SV14 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G005000 PE=3 SV=1)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 703/773 (90.94%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALK+LLKPKSSITKFFLSSLSFSSFCK QQQPIFSFP QSPPSPKKLPFTHSVHGV LQ
Sbjct: 13  MALKSLLKPKSSITKFFLSSLSFSSFCK-QQQPIFSFPPQSPPSPKKLPFTHSVHGVTLQ 72

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPD +DYLRQENLYAEAFMADT+VLQRQLFSEMT RIP+KVSTPPEPWGP
Sbjct: 73  DPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPEPWGP 132

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWF+KIL FGKGN GKEEQVLLDWNEIAK+YGYVH
Sbjct: 133 WFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRYGYVH 192

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGR+LFY
Sbjct: 193 VGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRMLFY 252

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300
           TQADENQRPYR            DVSVFVENDPNYCVDITSTKDGKF+TVYIIDANNSLG
Sbjct: 253 TQADENQRPYRQ-------TFVNDVSVFVENDPNYCVDITSTKDGKFVTVYIIDANNSLG 312

Query: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360
           GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKN  C EEDYYVARCRVEDIKSADWQDI
Sbjct: 313 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDYYVARCRVEDIKSADWQDI 372

Query: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420
           VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSIN PLDAD NHHLEI+KLDPWFFPLPS
Sbjct: 373 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADDNHHLEIEKLDPWFFPLPS 432

Query: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTN 480
           NSCSVAPGSNHDFMSS YRVVLSSPVMPDLIVDYDMSKR FSIIQQEEVK QHDVELKTN
Sbjct: 433 NSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFSIIQQEEVKVQHDVELKTN 492

Query: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540
           LPDTLD +EVSDTQ+KRENFQNC+SQNWKDFSEAY CERIEV SHDG+ IPLTILY+PMT
Sbjct: 493 LPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEVTSHDGVGIPLTILYTPMT 552

Query: 541 FQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600
           FQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR             G+G
Sbjct: 553 FQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRS------------GNG 612

Query: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPF 660
           LEKPNSIHDF+SCANFLI+NGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF AAILKVPF
Sbjct: 613 LEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFRAAILKVPF 672

Query: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720
           LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPY+NISKG+CYPSMLVTASFHD
Sbjct: 673 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYPSMLVTASFHD 732

Query: 721 ARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYE 774
           ARVGVWEAAKWVAKIRDTTCS CS+SAILKTNMLGGHFGEGGLYGGCEE AYE
Sbjct: 733 ARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGGLYGGCEEMAYE 765

BLAST of CSPI05G24490 vs. ExPASy TrEMBL
Match: A0A5D3CCK8 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00280 PE=3 SV=1)

HSP 1 Score: 1393.6 bits (3606), Expect = 0.0e+00
Identity = 679/773 (87.84%), Postives = 702/773 (90.82%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALK+LLKPKSSITKFFLSSLSFSSFCK QQQPIFSFP QSPPSPKKLPFTHSVHGV LQ
Sbjct: 13  MALKSLLKPKSSITKFFLSSLSFSSFCK-QQQPIFSFPPQSPPSPKKLPFTHSVHGVTLQ 72

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPD +DYLRQENLYAEAFMADT+VLQRQLFSEMT RIP+KVSTPPEPWGP
Sbjct: 73  DPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPEPWGP 132

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWF+KIL FGKGN GKEEQVLLDWNEIAK+YGYVH
Sbjct: 133 WFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRYGYVH 192

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGR+LFY
Sbjct: 193 VGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRMLFY 252

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300
           TQADENQRPYR                                   F+ VYIIDANNSLG
Sbjct: 253 TQADENQRPYR---------------------------------QTFVNVYIIDANNSLG 312

Query: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360
           GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKN  C EEDYYVARCRVEDIKSADWQDI
Sbjct: 313 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDYYVARCRVEDIKSADWQDI 372

Query: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420
           VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSIN PLDAD NHHLEI+KLDPWFFPLPS
Sbjct: 373 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADDNHHLEIEKLDPWFFPLPS 432

Query: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTN 480
           NSCSVAPGSNHDFMSS YRVVLSSPVMPDLIVDYDMSKR FSIIQQEEVK QHDVELKTN
Sbjct: 433 NSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFSIIQQEEVKVQHDVELKTN 492

Query: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540
           LPDTLD +EVSDTQ+KRENFQNC+SQNWKDFSEAY CERIEV SHDG+ IPLTILY+PMT
Sbjct: 493 LPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEVTSHDGVGIPLTILYTPMT 552

Query: 541 FQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600
           FQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR             G+G
Sbjct: 553 FQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRS------------GNG 612

Query: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPF 660
           LEKPNSIHDF+SCANFLI+NGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF AAILKVPF
Sbjct: 613 LEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFRAAILKVPF 672

Query: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720
           LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPY+NISKG+CYPSMLVTASFHD
Sbjct: 673 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYPSMLVTASFHD 732

Query: 721 ARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYE 774
           ARVGVWEAAKWVAKIRDTTCS CS+SAILKTNMLGGHFGEGGLYGGCEE AYE
Sbjct: 733 ARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGGLYGGCEEMAYE 739

BLAST of CSPI05G24490 vs. ExPASy TrEMBL
Match: A0A6J1GZW2 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458759 PE=3 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 665/800 (83.12%), Postives = 723/800 (90.38%), Query Frame = 0

Query: 1   MALKTLLKP-KSSITKFFLSSLS-FSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVK 60
           MALK+LLKP ++SI    +SS S FSS CK++   IFS PS+SPP+ KK+PFTHSVHG+ 
Sbjct: 1   MALKSLLKPTRNSILCSSISSSSLFSSLCKER---IFSLPSESPPAAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPW 120
           LQDPYHWM+NT DPD ADYLR+ENLYAEAFMADTQ+LQR+LFSEMTSRI  KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRISTKVSTPPEPW 120

Query: 121 GPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGY 180
           GPWFYYQYIP+GKEYPVLCRRLQNEK++W +K+  F KGNSGK+E+VLLDWNEIAKQYGY
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQYGY 180

Query: 181 VHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVL 240
           VHVGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLR+GL+IPKLQ EGVVSLAWAEEGR L
Sbjct: 181 VHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQ-EGVVSLAWAEEGRTL 240

Query: 241 FYTQADENQRPYRVFCTKVGFNDT-EDVSVFVENDPNYCVDITSTKDGKFIT-------- 300
           FYTQADENQRPYRVF TK+GF+DT EDV VFVENDPNYCVDITSTKDGKFIT        
Sbjct: 241 FYTQADENQRPYRVFSTKLGFSDTGEDVLVFVENDPNYCVDITSTKDGKFITVNSNSRTS 300

Query: 301 --VYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARC 360
             VYIIDANN L GLQRIH+RIPGIQYFLEHH GFFYILTNAPLEK G CS+EDYYVARC
Sbjct: 301 SEVYIIDANNWLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAPLEKKGDCSKEDYYVARC 360

Query: 361 RVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLE 420
           RVEDIKSA+WQDIVLQS+DFSI DMD+FSGHLVLFVNKNGV MLCSIN PLDA+H H LE
Sbjct: 361 RVEDIKSANWQDIVLQSKDFSIHDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKHRLE 420

Query: 421 IDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQE 480
           I+KLDPWFFPLPSNSCSVAPGSNHDF SSLYRVVLSSPVMPDLIVDYDMSKR+FSIIQQE
Sbjct: 421 IEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQE 480

Query: 481 EVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDG 540
           EV+ +HD++LKT  PD L  E+VSD Q+KRENF+  ES+ WKDFS++Y CER EV SHDG
Sbjct: 481 EVEVKHDIKLKTYQPDALGIEKVSDAQNKRENFETRESETWKDFSDSYCCERKEVISHDG 540

Query: 541 IRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGG 600
           IR+PLTILYSP TFQKG+SPG+LQGYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R GG
Sbjct: 541 IRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR-GG 600

Query: 601 GGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMH 660
           GGGDSSWHRCGSGL+K NSI DFI CANFLI NGYVHK+RLGSIGYSAGGLLVGAAINMH
Sbjct: 601 GGGDSSWHRCGSGLQKQNSIQDFIFCANFLIDNGYVHKNRLGSIGYSAGGLLVGAAINMH 660

Query: 661 PNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGT 720
           P+LF AAILKVPFLDICNTLLDPSLPLT+LDYEEFGNP+I  QFESILSYSPYDNISKG+
Sbjct: 661 PDLFGAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPEIAMQFESILSYSPYDNISKGS 720

Query: 721 CYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGC 780
           CYP MLVTASF DARVGVWEAAKWVAKIRDTTCS CSTSAILKTNM+GGHFGEGGLYGGC
Sbjct: 721 CYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLYGGC 780

Query: 781 EETAYEYAFLIKVLRTPDHD 788
           EETAY+YAFLIKVL T D D
Sbjct: 781 EETAYDYAFLIKVLGTSDRD 795

BLAST of CSPI05G24490 vs. NCBI nr
Match: XP_011655724.1 (uncharacterized protein LOC101218436 [Cucumis sativus])

HSP 1 Score: 1622.4 bits (4200), Expect = 0.0e+00
Identity = 782/797 (98.12%), Postives = 783/797 (98.24%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ
Sbjct: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP
Sbjct: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH
Sbjct: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY
Sbjct: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT----------V 300
           TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT          V
Sbjct: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVNSNSRTSSEV 300

Query: 301 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE 360
           YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE
Sbjct: 301 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE 360

Query: 361 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK 420
           DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK
Sbjct: 361 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK 420

Query: 421 LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK 480
           LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK
Sbjct: 421 LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK 480

Query: 481 AQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRI 540
            QHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRI
Sbjct: 481 VQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRI 540

Query: 541 PLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 600
           PLTILYSPMTF+KGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG
Sbjct: 541 PLTILYSPMTFKKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 600

Query: 601 DSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 660
           DSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL
Sbjct: 601 DSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 660

Query: 661 FCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYP 720
           F AAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYP
Sbjct: 661 FRAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYP 720

Query: 721 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEET 780
           SMLVTASFHDARVGVWEAAKWVAKIRDTTCS CSTSAILKTNMLGGHFGEGGLYGGCEE 
Sbjct: 721 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEEM 780

Query: 781 AYEYAFLIKVLRTPDHD 788
           AYEYAFLIKVLRTPDHD
Sbjct: 781 AYEYAFLIKVLRTPDHD 797

BLAST of CSPI05G24490 vs. NCBI nr
Match: XP_008446478.1 (PREDICTED: LOW QUALITY PROTEIN: protease 2 [Cucumis melo])

HSP 1 Score: 1545.0 bits (3999), Expect = 0.0e+00
Identity = 741/797 (92.97%), Postives = 764/797 (95.86%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALK+LLKPKSSITKFFLSSLSFSSFCK QQQPIFSFP QSPPSPKKLPFTHSVHGV LQ
Sbjct: 13  MALKSLLKPKSSITKFFLSSLSFSSFCK-QQQPIFSFPPQSPPSPKKLPFTHSVHGVTLQ 72

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPD +DYLRQENLYAEAFMADT+VLQRQLFSEMT RIP+KVSTPPEPWGP
Sbjct: 73  DPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPEPWGP 132

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWF+KIL FGKGN GKEEQVLLDWNEIAK+YGYVH
Sbjct: 133 WFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRYGYVH 192

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGR+LFY
Sbjct: 193 VGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRMLFY 252

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT----------V 300
           TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKF+T          V
Sbjct: 253 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFVTVNSNSRTSSEV 312

Query: 301 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE 360
           YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKN  C EEDYYVARCRVE
Sbjct: 313 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDYYVARCRVE 372

Query: 361 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK 420
           DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSIN PLDAD NHHLEI+K
Sbjct: 373 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADDNHHLEIEK 432

Query: 421 LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK 480
           LDPWFFPLPSNSCSVAPGSNHDFMSS YRVVLSSPVMPDLIVDYDMSKR FSIIQQEEVK
Sbjct: 433 LDPWFFPLPSNSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFSIIQQEEVK 492

Query: 481 AQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRI 540
            QHDVELKTNLPDTLD +EVSDTQ+KRENFQNC+SQNWKDFSEAY CERIEV SHDG+ I
Sbjct: 493 VQHDVELKTNLPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEVTSHDGVGI 552

Query: 541 PLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 600
           PLTILY+PMTFQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG
Sbjct: 553 PLTILYTPMTFQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGG 612

Query: 601 DSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 660
           DSSWHR G+GLEKPNSIHDF+SCANFLI+NGYVHKDRLGSIGYSAGGLLVGAAINMHPNL
Sbjct: 613 DSSWHRSGNGLEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGAAINMHPNL 672

Query: 661 FCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYP 720
           F AAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPY+NISKG+CYP
Sbjct: 673 FRAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYP 732

Query: 721 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEET 780
           SMLVTASFHDARVGVWEAAKWVAKIRDTTCS CS+SAILKTNMLGGHFGEGGLYGGCEE 
Sbjct: 733 SMLVTASFHDARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGGLYGGCEEM 792

Query: 781 AYEYAFLIKVLRTPDHD 788
           AYEYAFLIKVLRTPDHD
Sbjct: 793 AYEYAFLIKVLRTPDHD 808

BLAST of CSPI05G24490 vs. NCBI nr
Match: KAE8648773.1 (hypothetical protein Csa_008861 [Cucumis sativus])

HSP 1 Score: 1536.9 bits (3978), Expect = 0.0e+00
Identity = 745/787 (94.66%), Postives = 747/787 (94.92%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ
Sbjct: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP
Sbjct: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH
Sbjct: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY
Sbjct: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300
           TQADENQRPYR                                   F+ VYIIDANNSLG
Sbjct: 241 TQADENQRPYRQI---------------------------------FVNVYIIDANNSLG 300

Query: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360
           GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI
Sbjct: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360

Query: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420
           VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS
Sbjct: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420

Query: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTN 480
           NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK QHDVELKTN
Sbjct: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKVQHDVELKTN 480

Query: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540
           LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT
Sbjct: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540

Query: 541 FQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600
           F+KGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG
Sbjct: 541 FKKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600

Query: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPF 660
           LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF AAILKVPF
Sbjct: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFRAAILKVPF 660

Query: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720
           LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD
Sbjct: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720

Query: 721 ARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKV 780
           ARVGVWEAAKWVAKIRDTTCS CSTSAILKTNMLGGHFGEGGLYGGCEE AYEYAFLIKV
Sbjct: 721 ARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEEMAYEYAFLIKV 754

Query: 781 LRTPDHD 788
           LRTPDHD
Sbjct: 781 LRTPDHD 754

BLAST of CSPI05G24490 vs. NCBI nr
Match: KAA0034488.1 (protease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 703/773 (90.94%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MALK+LLKPKSSITKFFLSSLSFSSFCK QQQPIFSFP QSPPSPKKLPFTHSVHGV LQ
Sbjct: 13  MALKSLLKPKSSITKFFLSSLSFSSFCK-QQQPIFSFPPQSPPSPKKLPFTHSVHGVTLQ 72

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DPYHWMSNTHDPD +DYLRQENLYAEAFMADT+VLQRQLFSEMT RIP+KVSTPPEPWGP
Sbjct: 73  DPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPEPWGP 132

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           WFYYQYIPDGKEYPVLCRRLQNEKSSWF+KIL FGKGN GKEEQVLLDWNEIAK+YGYVH
Sbjct: 133 WFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRYGYVH 192

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VGTCR+SPDHNFLAYTVDITG+EHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGR+LFY
Sbjct: 193 VGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRMLFY 252

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVYIIDANNSLG 300
           TQADENQRPYR            DVSVFVENDPNYCVDITSTKDGKF+TVYIIDANNSLG
Sbjct: 253 TQADENQRPYRQ-------TFVNDVSVFVENDPNYCVDITSTKDGKFVTVYIIDANNSLG 312

Query: 301 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDI 360
           GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKN  C EEDYYVARCRVEDIKSADWQDI
Sbjct: 313 GLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDYYVARCRVEDIKSADWQDI 372

Query: 361 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPS 420
           VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSIN PLDAD NHHLEI+KLDPWFFPLPS
Sbjct: 373 VLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADDNHHLEIEKLDPWFFPLPS 432

Query: 421 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTN 480
           NSCSVAPGSNHDFMSS YRVVLSSPVMPDLIVDYDMSKR FSIIQQEEVK QHDVELKTN
Sbjct: 433 NSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFSIIQQEEVKVQHDVELKTN 492

Query: 481 LPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIPLTILYSPMT 540
           LPDTLD +EVSDTQ+KRENFQNC+SQNWKDFSEAY CERIEV SHDG+ IPLTILY+PMT
Sbjct: 493 LPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEVTSHDGVGIPLTILYTPMT 552

Query: 541 FQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSG 600
           FQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR             G+G
Sbjct: 553 FQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRS------------GNG 612

Query: 601 LEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPF 660
           LEKPNSIHDF+SCANFLI+NGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF AAILKVPF
Sbjct: 613 LEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFRAAILKVPF 672

Query: 661 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHD 720
           LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPY+NISKG+CYPSMLVTASFHD
Sbjct: 673 LDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYPSMLVTASFHD 732

Query: 721 ARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYE 774
           ARVGVWEAAKWVAKIRDTTCS CS+SAILKTNMLGGHFGEGGLYGGCEE AYE
Sbjct: 733 ARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGGLYGGCEEMAYE 765

BLAST of CSPI05G24490 vs. NCBI nr
Match: XP_038892765.1 (protease 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 687/804 (85.45%), Postives = 727/804 (90.42%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFL-----SSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVH 60
           MALK+LLKPKSSITKFFL     SS  FSS CK   +PIFS PSQSPP+ KKLPFTHSVH
Sbjct: 1   MALKSLLKPKSSITKFFLYSSIASSSRFSSSCK---KPIFSLPSQSPPTAKKLPFTHSVH 60

Query: 61  GVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPP 120
           GV LQD YHWMSNTHDPD ADYLRQEN YAEAFM DTQ+LQ++LFSEMTSRIPAKVSTPP
Sbjct: 61  GVTLQDSYHWMSNTHDPDLADYLRQENFYAEAFMGDTQILQQRLFSEMTSRIPAKVSTPP 120

Query: 121 EPWGPWFYYQYIPDGKEYPVLCRRLQ--NEKSSWFRKILNFGKGNSGKEEQVLLDWNEIA 180
           EPWGPWFYYQYIP+GKEYPVLCRRLQ  NEKSSW +K+L F KGN GKEEQVLLDWNEIA
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLQNENEKSSWLKKLLQFVKGNFGKEEQVLLDWNEIA 180

Query: 181 KQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAE 240
           KQYGYVHVGTCR+SPDHNFLAYTVDITG+EHFMLQIKDL +G IIP+LQKEGVVSLAWAE
Sbjct: 181 KQYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLGSGRIIPELQKEGVVSLAWAE 240

Query: 241 EGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT---- 300
           EGR+LFYTQAD NQRPYRVFCTKVGF DTEDVSVFVENDPNYCVDITSTKDGKF+T    
Sbjct: 241 EGRMLFYTQADGNQRPYRVFCTKVGFKDTEDVSVFVENDPNYCVDITSTKDGKFVTVNSN 300

Query: 301 ------VYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYY 360
                 VYIIDA NS+ GLQRIH+R+PGIQYFLEHHHGFFYILTNAPLEKNG C +EDYY
Sbjct: 301 SRTSSEVYIIDAKNSVSGLQRIHKRVPGIQYFLEHHHGFFYILTNAPLEKNGDCLKEDYY 360

Query: 361 VARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHN 420
           VARCRVEDIKSAD QDI+LQSEDFSIQDMD+F GHLVLFVNKNGV MLCSIN PLD +  
Sbjct: 361 VARCRVEDIKSADQQDIILQSEDFSIQDMDLFGGHLVLFVNKNGVPMLCSINLPLDVNDK 420

Query: 421 HHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSI 480
             +EI KLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMS+R+FSI
Sbjct: 421 QCIEIKKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSRRVFSI 480

Query: 481 IQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVK 540
           IQQEEVK +  V+LKT LPD LD  EVSD Q+KRENFQN ESQNWKDFS+ Y+CER EV 
Sbjct: 481 IQQEEVKVKPGVKLKTYLPDALDIGEVSDAQNKRENFQNSESQNWKDFSDVYYCERKEVI 540

Query: 541 SHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADV 600
           SHDGIRIPLTILYSPM FQKGQSPG+LQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADV
Sbjct: 541 SHDGIRIPLTILYSPMNFQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADV 600

Query: 601 RGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAA 660
           RGG GGGDSSWHRCGSGLEK NSI DFISCANFL++NGY+HKDRLGSIGYSAGGLLVGAA
Sbjct: 601 RGGSGGGDSSWHRCGSGLEKQNSILDFISCANFLVNNGYIHKDRLGSIGYSAGGLLVGAA 660

Query: 661 INMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNI 720
           INMHP+LF AAILKVPFLD+CNTLLDP+LPLT+LDYEEFG+PQI KQF SILSYSPYDNI
Sbjct: 661 INMHPDLFRAAILKVPFLDVCNTLLDPTLPLTILDYEEFGSPQIPKQFRSILSYSPYDNI 720

Query: 721 SKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGL 780
           SKG+CYP MLVTAS  DARVGVWEAAKWVAKIRDTTCS CSTSAILKTNMLGGHFGEGGL
Sbjct: 721 SKGSCYPPMLVTASLRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGL 780

Query: 781 YGGCEETAYEYAFLIKVLRTPDHD 788
           YGGCEETAYEYAFLIKVL T DHD
Sbjct: 781 YGGCEETAYEYAFLIKVLGTSDHD 801

BLAST of CSPI05G24490 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 883.2 bits (2281), Expect = 1.5e-256
Identity = 438/795 (55.09%), Postives = 555/795 (69.81%), Query Frame = 0

Query: 1   MALKTLLKPKSSITKFFLSSLSFSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVHGVKLQ 60
           MA+  LL+     T    S LSFS+ C   +    S P+++PP PKK+PF  S HG+  Q
Sbjct: 1   MAVTYLLRRYKPAT--ISSVLSFSTKCFVGRTSSLSVPTEAPPVPKKIPFAISSHGITRQ 60

Query: 61  DPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGP 120
           DP+HWM NT D DF D+L++EN Y++AFMADT+ L+R LFSEM +RIP ++ TPPE WG 
Sbjct: 61  DPFHWMKNTDDTDFVDFLKRENSYSQAFMADTETLRRDLFSEMKTRIPEEIFTPPERWGQ 120

Query: 121 WFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVH 180
           W Y QYIP GKEYP+LCRRL+  K++W   +        G+EE+V+LDWN+IA+Q+GYVH
Sbjct: 121 WLYRQYIPKGKEYPLLCRRLEKGKTNWLSGLFR------GEEEEVVLDWNQIAEQFGYVH 180

Query: 181 VGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAWAEEGRVLFY 240
           VG CR+SPDHN+LAYTVD  G                                +G  LFY
Sbjct: 181 VGVCRVSPDHNYLAYTVDPEG--------------------------------DGITLFY 240

Query: 241 TQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFIT----------V 300
           T  DENQRP+RV  T V  +  +D  VF E D ++CVDIT+TKDGKF+T          V
Sbjct: 241 TVTDENQRPHRVVVTNVESDGRDDAVVFTERDSSFCVDITTTKDGKFVTINSNSRTSSEV 300

Query: 301 YIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVE 360
           YI++A+  + GLQR  ER+PG+Q FLEHH+GFFYILTN+P       S E YY+ RC VE
Sbjct: 301 YIVNADKPMAGLQRTRERVPGVQCFLEHHNGFFYILTNSPSNAISEWSGEGYYLTRCLVE 360

Query: 361 DIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDK 420
           +I+++DWQ +    +D  IQDMD+F+ +LVL++NK G+ MLCSI+ P+ A+  H   +D 
Sbjct: 361 EIEASDWQTVFRPDDDVVIQDMDMFNDYLVLYLNKKGLPMLCSIDMPIKANTKH---MDD 420

Query: 421 LDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVK 480
           L PW+FPLP +SCSVAPGSNHDF SS+YRVVLSSPV+PD IVDYD+S+R+FSI+QQE   
Sbjct: 421 LVPWYFPLPVDSCSVAPGSNHDFQSSIYRVVLSSPVIPDTIVDYDVSRRLFSIVQQEGGV 480

Query: 481 AQHDVELK---TNLPDTLDAEEVSDTQSKRENFQ-NCESQNWKDFSEAYFCERIEVKSHD 540
             +    K   T    T +  +++D  S+ E+ Q +     W+D S+ Y CER EV SHD
Sbjct: 481 VDNSDSSKPWYTADRSTENNGQLNDRTSEGEDGQLDSRMPKWEDLSDTYVCERQEVSSHD 540

Query: 541 GIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGG 600
           G+ +PLTILYS   ++K +SPG+L GYGAYGE+LDKSWC  RLS+LDRG+V+AFADVR G
Sbjct: 541 GVEVPLTILYSREAWKKSESPGMLIGYGAYGEVLDKSWCTNRLSMLDRGWVIAFADVR-G 600

Query: 601 GGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINM 660
           GG G+ SWH+ G+   K NSI DFI  A +L+  GYVH+  L ++GYSAG +L  AA+NM
Sbjct: 601 GGSGEFSWHKSGTRSLKQNSIQDFIYSAKYLVEKGYVHRHHLAAVGYSAGAILPAAAMNM 660

Query: 661 HPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKG 720
           HP+LF A ILKVPF+D+ NTL DP+LPLT+LD+EEFGNP  Q  F SILSYSPYD I K 
Sbjct: 661 HPSLFQAVILKVPFVDVLNTLSDPNLPLTLLDHEEFGNPDNQTDFGSILSYSPYDKIRKD 720

Query: 721 TCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGG 780
            CYPSMLVT SFHD+RVGVWE AKWVAKIRD+TC  CS + ILKTNM GGHFGEGG Y  
Sbjct: 721 VCYPSMLVTTSFHDSRVGVWEGAKWVAKIRDSTCHDCSRAVILKTNMNGGHFGEGGRYAQ 751

Query: 781 CEETAYEYAFLIKVL 782
           CEETA++YAFL+KV+
Sbjct: 781 CEETAFDYAFLLKVM 751

BLAST of CSPI05G24490 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-153
Identity = 305/768 (39.71%), Postives = 437/768 (56.90%), Query Frame = 0

Query: 38  PSQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDP----DFADYLRQENLYAEAFMADTQ 97
           P  + P P K P + + H    +DPY WMS   D         Y+ QE  Y EA +ADT 
Sbjct: 35  PPPALPKPPKKPQSFTFHDATWEDPYSWMSKLEDKVAMRHMDIYMEQEEKYTEAVLADTD 94

Query: 98  VLQRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRR---LQNEKSSWFRK 157
            +Q +L SEM SR+  ++STPP  WGPW YY+ + +GK+YPVLCRR   L  E  S    
Sbjct: 95  RIQTKLQSEMASRLSFELSTPPLRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISHKSP 154

Query: 158 ILNFGKGNSGKEEQVLLDWNEIAKQY-GYVHVGTCRISPDHNFLAYTVDITGNEHFMLQI 217
              F   +  + EQ LLD+N+ A+++ GY +     ISPDH FLAYT+    N++F L +
Sbjct: 155 AAGFDYTSGKRIEQKLLDYNQEAERFGGYAYEEMSEISPDHKFLAYTMYDKDNDYFKLCV 214

Query: 218 KDLRNGLIIPKLQKEGVVSLAWAEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFV 277
           ++L +G +  K   + V ++AWA+ G+ L Y   D+ +RP R++C+ +G  D EDV +  
Sbjct: 215 RNLNSGALCSKPHADRVSNIAWAKNGQALLYVVTDQKKRPCRIYCSTIGSTD-EDVLLHE 274

Query: 278 ENDPNYCVDITSTKDGKFIT----------VYIIDANNSLGGLQRIHERIPGIQYFLEHH 337
           E + N  V+I  TKD  F+T          V++I+A +   GL  + E        +EHH
Sbjct: 275 EFEGNVHVNIRHTKDFHFVTVNTFSTTFSKVFLINAADPFSGLALVWEHNAPAHCIIEHH 334

Query: 338 HGFFYILTNAPLEKNGGCSEEDYYVARCRVE-DIKSADWQDIVLQSEDFSIQDMDIFSGH 397
            GF Y+ TNA    N G + + +Y+ R  V        W+ + +   +  I+D+D    H
Sbjct: 335 QGFLYLFTNA---SNDGGTVDHHYLLRSPVHFSSCQRIWETVFINDPELIIEDVDFCKKH 394

Query: 398 LVLFVNKNGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLY 457
           L L V +     +C ++ PL       + +  + P + PLP +   + PG+N+DF S   
Sbjct: 395 LSLIVKEMQSFKICVVDLPLKT-KRVPVHLRDIKPRYLPLPKHVSQIFPGTNYDFNSPTM 454

Query: 458 RVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRE 517
           R  +SS VMPD +VDYD+    ++I+QQ+ +  +    L      T      S T++   
Sbjct: 455 RFTISSLVMPDAVVDYDLLNGKWNIVQQQNMLHERTRVLYGTANSTESPNIPSGTRTVSF 514

Query: 518 NFQNCESQN---WKDFSEAYFCERIEVKSHDGIRIPLTILYSPMTFQKGQSPGILQGYGA 577
           + ++  ++N   W D +E Y C+  EV SHDG  +PL+I+YS    ++ Q PG+L  +GA
Sbjct: 515 DTEDTTAENDNLWNDLTEFYACDYHEVSSHDGAMVPLSIVYSRAQKEENQKPGLLHVHGA 574

Query: 578 YGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCAN 637
           YGE+LDK W     SLLDRG+VLA+ADVRGGGG G   WH+ G G +K NSI D+I CA 
Sbjct: 575 YGEMLDKRWRSELKSLLDRGWVLAYADVRGGGGKG-KKWHQDGRGAKKLNSIKDYIQCAK 634

Query: 638 FLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLT 697
           +L+ N  V +++L   GYSAGGL+V +AIN  P+LF AA+LKVPFLD  +TL+ P LPLT
Sbjct: 635 YLVENNIVEENKLAGWGYSAGGLVVASAINHCPDLFQAAVLKVPFLDPTHTLIYPILPLT 694

Query: 698 VLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKI 757
             DYEEFG P     F +I  YSPYDNI K   YP++LVT+SF + R GVWEAAKWVA++
Sbjct: 695 AEDYEEFGYPGDINDFHAIREYSPYDNIPKDVLYPAVLVTSSF-NTRFGVWEAAKWVARV 754

Query: 758 RDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLRT 784
           RD T +      +L    L     E   +   +E+A E AFLIK++ +
Sbjct: 755 RDNTFNDPERPVLLN---LTTDIVEENRFLQTKESALEIAFLIKMMES 792

BLAST of CSPI05G24490 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 345.5 bits (885), Expect = 1.1e-94
Identity = 231/757 (30.52%), Postives = 367/757 (48.48%), Query Frame = 0

Query: 39  SQSPPSPKKLPFTHSVHGVKLQDPYHWM--SNTHDPDFADYLRQENLYAEAFMADTQVLQ 98
           S+SPP  KK+     + G    D Y+W+   +  +PD   YLR+EN Y +  M+ T+  +
Sbjct: 4   SRSPPVAKKVEHVMEMFGDVRVDNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTKQFE 63

Query: 99  RQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGK 158
            QLF+E+  RI     + P   GP++YY+    GKEY   CRRL  +  +          
Sbjct: 64  NQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEPSVYDTMPT 123

Query: 159 GNSGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGL 218
           G     E V+LD N  A+++ Y  +G  + SPDH  +AY  D  G+E + + + D    L
Sbjct: 124 GPDAPPEHVILDENTKAQEHDYYRIGAFKASPDHKLVAYAEDTKGDEIYTVNVID-SEAL 183

Query: 219 IIPKLQKEGVVS-LAWAEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNY 278
                Q +G+ S L WA    +L+ T  DE  RP +V+  K+G   + DV ++ E D  +
Sbjct: 184 KPVGQQLKGLTSYLEWAGNDALLYITM-DEILRPDKVWLHKLGTEQSSDVCLYHEKDDMF 243

Query: 279 CVDITSTKDGKFI----------TVYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYI 338
            +++ +++  K++           V+ +D + +  GL+ +  R+ GI   + H    F+I
Sbjct: 244 SLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRVDGIDSSVSHRGNHFFI 303

Query: 339 LTNAPLEKNGGCSEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNK 398
              +    N         +  C V+D  ++    ++   E   IQ++ +F  HL +F  +
Sbjct: 304 QRRSTEFYNS-------ELIACPVDD--TSKTTVLLPHRESVKIQEIQLFRDHLAVFERE 363

Query: 399 NGVSMLCSINFPLDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSP 458
           NG+  +     P +      L+  +   +  P+ S        +  +F S + R    S 
Sbjct: 364 NGLQKITVHRLPAEGQPLEGLQGGRNVSFVDPVYS-----VDSTESEFSSRVLRFKYCSM 423

Query: 459 VMPDLIVDYDMSKRIFSIIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCES 518
             P  + DYDM      + + + V    D                               
Sbjct: 424 KTPPSVYDYDMDSGTSVVKKIDTVLGGFD------------------------------- 483

Query: 519 QNWKDFSEAYFCERIEVKSHDGIRIPLTILYS-PMTFQKGQSPGILQGYGAYGEILDKSW 578
                 +  Y  ER  V + DG +IP++I+Y+  +    G  P +L GYG+Y   +D  +
Sbjct: 484 ------ASNYVTERKWVAASDGTQIPMSIVYNKKLAKLDGSDPLLLYGYGSYEISVDPYF 543

Query: 579 CPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVH 638
              RLSLLDRGF    A VRGGG  G   W+  G  L+K N+  DFI+CA  LI   Y  
Sbjct: 544 KASRLSLLDRGFTFVIAHVRGGGEMG-RQWYENGKLLKKKNTFTDFIACAERLIELKYCS 603

Query: 639 KDRLGSIGYSAGGLLVGAAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGN 698
           K++L   G SAGGLL+GA +NM P+LF   I  VPF+D+  T+LDP++PLT  ++EE+G+
Sbjct: 604 KEKLCMEGRSAGGLLMGAVVNMRPDLFKVVIAGVPFVDVLTTMLDPTIPLTTSEWEEWGD 663

Query: 699 PQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCS 758
           P+ ++ +  + SYSP DN++    YP+MLVTA  +D RV   E  KWVAK+R+      +
Sbjct: 664 PRKEEFYFYMKSYSPVDNVTAQN-YPNMLVTAGLNDPRVMYSEPGKWVAKLREMKTD--N 703

Query: 759 TSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 782
              + K  +  GHF + G +   +E A+ +AF++KVL
Sbjct: 724 NVLLFKCELGAGHFSKSGRFEKLQEDAFTFAFMMKVL 703

BLAST of CSPI05G24490 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 144.1 bits (362), Expect = 4.9e-34
Identity = 172/813 (21.16%), Postives = 319/813 (39.24%), Query Frame = 0

Query: 19  SSLSFSSFCKQQ----QQPIFSFPSQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDF 78
           +S+S   +C          +F    Q P + +        HGVK+ DPY W+ +    + 
Sbjct: 51  NSVSRRCYCSSSAIMGSSSVFGEQLQYPATRRDDSVVDDYHGVKIGDPYRWLEDPDAEEV 110

Query: 79  ADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYP 138
            ++++ +    ++ +   +  + +L   +T  I       P   G  ++Y +    +   
Sbjct: 111 KEFVQSQVKLTDSVLEKCET-KEKLRQNITKLIDHPRYDSPFRQGDKYFYFHNTGLQAQS 170

Query: 139 VLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLA 198
           VL                 + + N   E +VLLD N ++   G V + T  +S D  +LA
Sbjct: 171 VL-----------------YMQDNLDAEPEVLLDPNTLSDD-GTVALNTFSVSEDAKYLA 230

Query: 199 YTVDITGNEHFMLQIKDLRNGLIIP-KLQKEGVVSLAWAEEGRVLFY------------- 258
           Y +  +G++   +++  + +  + P  L       + W  + +  FY             
Sbjct: 231 YGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYGRYPAPKEGEDID 290

Query: 259 --TQADENQRPYRVFCTKVGFNDTEDVSVFVEND-PNYCVDITSTKDGKFI--------- 318
             T+ + N   + ++   +G + ++D+  + +N+ P Y      T DGK++         
Sbjct: 291 AGTETNSNLY-HELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGKYLIMSIGESCD 350

Query: 319 ---TVYIIDANNSLGGLQRIH---ERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDY 378
               +Y  D  +  GGL+        +P I+  ++     + +++N         +++  
Sbjct: 351 PVNKLYYCDMTSLSGGLESFRGSSSFLPFIK-LVDTFDAQYSVISNDETLFTFLTNKDAP 410

Query: 379 YVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADH 438
                RV+  +   W D+V + E   +      +G+ ++    + V  +  I        
Sbjct: 411 KYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHILQIRDLKSGSL 470

Query: 439 NHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFS 498
            H L +D        + S S   A   ++ F  S      +S + P +I   D++     
Sbjct: 471 LHQLPLD--------IGSVSDVSARRKDNTFFFS-----FTSFLTPGVIYKCDLAN---- 530

Query: 499 IIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEV 558
             +  EVK                  EV+     RE FQ  +               +  
Sbjct: 531 --ESPEVKV---------------FREVTVPGFDREAFQAIQ---------------VFY 590

Query: 559 KSHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDR-GFVLAFA 618
            S DG +IP+ I+        G  P +L  YG +   +  S+   R+ L    G V  FA
Sbjct: 591 PSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFA 650

Query: 619 DVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVG 678
           ++RGGG  G+  WH+ GS  +K N   DFIS A +L+  GY    +L   G S GGLLVG
Sbjct: 651 NIRGGGEYGE-EWHKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVG 710

Query: 679 AAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYD 738
           A IN  P+L+  A+  V  +D+   L      +      ++G  + +++F  ++ YSP  
Sbjct: 711 ACINQRPDLYGCALAHVGVMDM---LRFHKFTIGHAWTSDYGCSENEEEFHWLIKYSPLH 770

Query: 739 NISKG--------TCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTS-----AI 782
           N+ +           YPS ++  + HD RV    + K +A ++   C+    S      I
Sbjct: 771 NVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLATLQHVLCTSLDNSPQMNPII 789

BLAST of CSPI05G24490 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 140.6 bits (353), Expect = 5.5e-33
Identity = 171/810 (21.11%), Postives = 318/810 (39.26%), Query Frame = 0

Query: 19  SSLSFSSFCKQQ----QQPIFSFPSQSPPSPKKLPFTHSVHGVKLQDPYHWMSNTHDPDF 78
           +S+S   +C          +F    Q P + +        HGVK+ DPY W+ +    + 
Sbjct: 51  NSVSRRCYCSSSAIMGSSSVFGEQLQYPATRRDDSVVDDYHGVKIGDPYRWLEDPDAEEV 110

Query: 79  ADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPPEPWGPWFYYQYIPDGKEYP 138
            ++++ +    ++ +   +  + +L   +T  I       P   G  ++Y +    +   
Sbjct: 111 KEFVQSQVKLTDSVLEKCET-KEKLRQNITKLIDHPRYDSPFRQGDKYFYFHNTGLQAQS 170

Query: 139 VLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQYGYVHVGTCRISPDHNFLA 198
           VL                 + + N   E +VLLD N ++   G V + T  +S D  +LA
Sbjct: 171 VL-----------------YMQDNLDAEPEVLLDPNTLSDD-GTVALNTFSVSEDAKYLA 230

Query: 199 YTVDITGNEHFMLQIKDLRNGLIIP-KLQKEGVVSLAWAEEGRVLFY------------- 258
           Y +  +G++   +++  + +  + P  L       + W  + +  FY             
Sbjct: 231 YGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYGRYPAPKEGEDID 290

Query: 259 --TQADENQRPYRVFCTKVGFNDTEDVSVFVEND-PNYCVDITSTKDGKFI--------- 318
             T+ + N   + ++   +G + ++D+  + +N+ P Y      T DGK++         
Sbjct: 291 AGTETNSNLY-HELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGKYLIMSIGESCD 350

Query: 319 ---TVYIIDANNSLGGLQRIH---ERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDY 378
               +Y  D  +  GGL+        +P I+  ++     + +++N         +++  
Sbjct: 351 PVNKLYYCDMTSLSGGLESFRGSSSFLPFIK-LVDTFDAQYSVISNDETLFTFLTNKDAP 410

Query: 379 YVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADH 438
                RV+  +   W D+V + E   +      +G+ ++    + V  +  I        
Sbjct: 411 KYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHILQIRDLKSGSL 470

Query: 439 NHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFS 498
            H L +D        + S S   A   ++ F  S      +S + P +I   D++     
Sbjct: 471 LHQLPLD--------IGSVSDVSARRKDNTFFFS-----FTSFLTPGVIYKCDLAN---- 530

Query: 499 IIQQEEVKAQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEV 558
             +  EVK                  EV+     RE FQ  +               +  
Sbjct: 531 --ESPEVKV---------------FREVTVPGFDREAFQAIQ---------------VFY 590

Query: 559 KSHDGIRIPLTILYSPMTFQKGQSPGILQGYGAYGEILDKSWCPYRLSLLDR-GFVLAFA 618
            S DG +IP+ I+        G  P +L  YG +   +  S+   R+ L    G V  FA
Sbjct: 591 PSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFA 650

Query: 619 DVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVG 678
           ++RGGG  G+  WH+ GS  +K N   DFIS A +L+  GY    +L   G S GGLLVG
Sbjct: 651 NIRGGGEYGE-EWHKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVG 710

Query: 679 AAINMHPNLFCAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYD 738
           A IN  P+L+  A+  V  +D+   L      +      ++G  + +++F  ++ YSP  
Sbjct: 711 ACINQRPDLYGCALAHVGVMDM---LRFHKFTIGHAWTSDYGCSENEEEFHWLIKYSPLH 770

Query: 739 NISKG--------TCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHC--STSAILKT 782
           N+ +           YPS ++  + HD RV    + K +A +  T+  +       I + 
Sbjct: 771 NVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLAHVLCTSLDNSPQMNPIIGRI 786

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q595365.8e-9630.68Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
P245553.0e-8428.19Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
O078346.6e-8428.42Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
P556562.0e-5624.65Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
P556277.6e-5624.24Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
A0A0A0KQZ90.0e+0099.36Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G606880 PE=3 SV=1[more]
A0A1S3BF530.0e+0092.97Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103489202 PE=3 SV=1[more]
A0A5A7SV140.0e+0090.94Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G... [more]
A0A5D3CCK80.0e+0087.84Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475... [more]
A0A6J1GZW20.0e+0083.13Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458759 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011655724.10.0e+0098.12uncharacterized protein LOC101218436 [Cucumis sativus][more]
XP_008446478.10.0e+0092.97PREDICTED: LOW QUALITY PROTEIN: protease 2 [Cucumis melo][more]
KAE8648773.10.0e+0094.66hypothetical protein Csa_008861 [Cucumis sativus][more]
KAA0034488.10.0e+0090.94protease 2 [Cucumis melo var. makuwa][more]
XP_038892765.10.0e+0085.45protease 2 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G69020.11.5e-25655.09Prolyl oligopeptidase family protein [more]
AT5G66960.13.1e-15339.71Prolyl oligopeptidase family protein [more]
AT1G50380.11.1e-9430.52Prolyl oligopeptidase family protein [more]
AT1G76140.14.9e-3421.16Prolyl oligopeptidase family protein [more]
AT1G76140.25.5e-3321.11Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 547..565
score: 32.69
coord: 602..621
score: 37.11
coord: 690..705
score: 41.12
coord: 709..731
score: 44.16
coord: 573..597
score: 36.42
coord: 632..652
score: 55.39
NoneNo IPR availableGENE3D2.130.10.120coord: 110..468
e-value: 1.7E-181
score: 607.1
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 28..786
NoneNo IPR availablePANTHERPTHR11757:SF12PROLYL OLIGOPEPTIDASE FAMILY PROTEINcoord: 28..786
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 38..466
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 42..459
e-value: 5.6E-56
score: 190.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 61..779
e-value: 1.7E-181
score: 607.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 517..738
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 568..781
e-value: 4.8E-46
score: 157.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G24490.1CSPI05G24490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity