CSPI05G23850 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G23850
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	transcription termination factor MTEF1, chloroplastic
Location	Chr5: 23748134 .. 23751591 (-)
RNA-Seq Expression	CSPI05G23850
Synteny	CSPI05G23850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGATATTTTGGTCCAATTTGTTCATCTTCTTCATTGAGCAGCAAAAAGCTTGGGTCTTTGAGGATGATAAGCAGACTCCCACTTCCTCCCTCAAGCTTCTTCTTCTTCTTCTTTCCTCCCTCATTGGCCGGGTCTCTTAAACCTTTGACAAACCCTAGTTTCAGAAAACCTCGTTTCACCCTTTCTCTCGATACCCAATTTTCTGACCGCTTCGACTCCGGCCTACTTTTCCGTGAGAAAATACTCTACCTTGAGAACCACCTCAATGTCGATTCTCGCAAAGCCTTCCGGGAGAATCCCCATTGTCGCTCTGCTACTCTCTCCACTCTCAAATCCGTCGAGGTCTGTCTCTCTTCCATGGGACTTGACCGATCTGCAGTCAGCCGCGTTCTCGATATGCACCCCAAACTCCTGACCTCGAATCCGGACTATGATATTTACCCCATCTTTGATTTTCTTCTTAATGAAGTTGAAATTCCATTTCCCGACATTCGTAAATCTATAATTCGATGCCCTAGAATTCTCGTCTCCGACTTGGACCATCAATTGCGACCTGCTTTGAAGTTCTTGCGTGACCTGGGTTTTGTTGGGTTGAAAGCCATCACGTGCCAGACGACATTGCTACTGGTTTCGAGTGTTGAACATACCCTTTTGCCTAAGATTCAGTACCTTGAGAGCTTGGGCTTATCGCATGAGGATGTGGTTAATATGGTGTTAAGGTCTCCGGGTTTGTTGACTTATAGCATTCAAAACAATTTGGTTCCTAAAGTGAGTTATTTCTTAGGAGACATGAAGGGTGATTTGTTGGAATTGAAACGGTTTCCGCAATATTTTTCGTTTAACTTGGAGAGGAAGATTAAGCTGCGTCATCGTTCACTGGTTGAACATGGTTTGTCACTTCCTTTGTCGAAAATGCTGAAGGCTAGTGACGGGGAGTTCAATGCATGGTTGATAGAAATGCGGTGCAGTTCGTTGATGGCTGATCGTTGAAGTGAGTTGCTTTGCTTCCTGTAGTTTTGTAGTTTCTTTGGATGAGATTATTTAGGATGCTGCTTTTCTCTGTACAGTTCGGCAAATCATTCCCTGTTTTTCCCTCCCTGGTGAGATAAAAAAAAGTATTTCGCTTAATTACATTGTTCATATGATAATTGTGAGAAACAAGAAGTGAGTTTGGCAGGTCTTGTAAAATATGCTAATTGTGGAAGACTACAACTTTGAATCAATGCATTGTTACTTCCTGTCAAAAAGTATGGAAAATTATCGATAATAGCAAGTTCAACAACTGATTCGTGGTGTAGTTATTGAGTGAACTTTCATGTGATATTAGTAAGCTATGCTCATTTACTTCAGCCAGATTATACTCGCAGTTCGTTTCTATGTGGATCCTAGGAGGTGAACATGTGGTTGATTTCTATTTTGATTTTGGACTAAGGGATATCTATCTGAAATCTACTCGTGTTGCACTTTATGATGCAAGATTTTGATTGATGCTACAGCATCTTTATAATTTGTTCACTGTCTAGCTTCCAAGGGATTGAATTCAATCGTCTTATGTTTAGTTTCAATAACTCCAACAAATTTTGTTTTTACCAACAGTTTTGAACATTTTTTAATCGTGGGATTGACTTCGTCTGCTGTACTTAAACTAAAGATAACAGATGCTGCATAGCTCCAAACACACGTGGTTCTTAATATTGTGCTAAAAATTGAAATTCACATCTTGCTATTGAGATAAAAATGGTATTTTTTAGACAAATTACACAATGCTTCACTTGCACTAACGAAAATACATGGATCATTGTATTAGGGGTTATATTATGTTTCTGAGTTATTCTTCACTCAGTGGAGTTCAACATGTTTTGAATGAAAATTCGATGGTTGTGGTCAAGAAGATATGATGGAGAAATTTTTTTCTGTTTTATTTTACATACACCCTGGAACTGGAATCCCCAGCTGTCCCATACACACGTGCTGCTATGTGGAAATCCTGTAGAAACATGGCTGATGTTCGTTTATGTGAACTTTTTCCTTCAATTGTGCAGGAACTGTGGTGGAAATGATTCTACAGCTCGATCGACAAGACCAGGCATTCTACCATATTCATCTTTTAATGCTGACCTTGACTATCTAAATTGGTTGGGCTGGAGGGATTCACACGATTGTTCCCATCTTGAGTGAGATCATCAGTCAGAATGTTTGCTATTTAAACAGAGAATACGTCGAAGACAGAGACTCAACGCTTCCACAATTACCATTTGTTGATGCAATGCCGGCCCTGACTACTGTAATTTGTTGAGAACTTTGGAGACTTGCAAGTTTGGCCCCCATCCCAAATGACTGCATCACATCTGGAAGATTGCTGTCTAAGTAGAAAATATCGTGAAAACTATGCGATTCCCCTTGAACCCCTACCATCAATTGAATTTAATTTCCTATGAGAGGCATTGTAAGAACACTCAACCTGTATATTCTAAATCTTTGACCATTGTTTGAAATTCATCAGAAAGAACATGTGGTTGGGTTTGCAAAAGGCTCAAACTTCAAGGAAGAAGAAAGGGAAAGATAGGGTGAAGTGAACCCCCAAAAGGCCCAAATCATATGATAACTTAAGGCAATGTACACCTCCATTCTGGGGGCTATAAATGCAGTCTACTGAAATCATCAGTAGGACTTGAATTATATTTTTCTGGTGGAAGAAAAGTTAGTGGTGTTGAAAATGAAACTAGATTTTGTCCCACCAAAGTTAGAAACAATGAGAGATTGATTTCATAATGCATGATGATTCATTTTCTTGTTGAAACATGAGCCGAAGGACACGGTTTCTTGCTGCAGAAATGCATCACTGTTCAGTAGTCCAGATTCCAGGGGTACGTTGTCCATTACATTACATGACAAGTTTTGAAGGGTACAGTATGTATGAAAGGCCTTGGGGTCATCTCTGTTTCTCGGGACAACAAATGCAAATGCAAATGCAAAATTTGGGGTCAGCATTCGTCACCTCTGGACCCCTTTACCTTCCTACAATCAATTTGCTCAGACACAGAAAAACAAGAGAGAAGGCATCTGTCCCATTCTTTAATCATTCACCCTCGAAGGATGGGATGAGTTTTTGATTTGAAATTCATGGTCTTCATTTAATTCTGTCTCTCTCTGCTCTACTCTACTCTTAAACAGTATTCTATTTATATTATGGTTGGTTGCTTCTAAGACAGATCTGTCTCAGAGGAGAATGAATGTTCTACGTAAGTCAAGATGGTTTCAACTTTCAATACTTGGATTAATTTTGGTTTATACATTAGATGTTAAGATAGAAATGGTTTTTGATTTGTTGGCATGAGATATTATTGTAAATGCTTATCTCGGATGGAAGAGTAGGCCAAAGAGAATTCATAGACAGTGAAATTATCTGTTATGAGACAGCAATTTCAGAATCCACATGTTATGAATTTATGGTTGT

mRNA sequence

GAGATATTTTGGTCCAATTTGTTCATCTTCTTCATTGAGCAGCAAAAAGCTTGGGTCTTTGAGGATGATAAGCAGACTCCCACTTCCTCCCTCAAGCTTCTTCTTCTTCTTCTTTCCTCCCTCATTGGCCGGGTCTCTTAAACCTTTGACAAACCCTAGTTTCAGAAAACCTCGTTTCACCCTTTCTCTCGATACCCAATTTTCTGACCGCTTCGACTCCGGCCTACTTTTCCGTGAGAAAATACTCTACCTTGAGAACCACCTCAATGTCGATTCTCGCAAAGCCTTCCGGGAGAATCCCCATTGTCGCTCTGCTACTCTCTCCACTCTCAAATCCGTCGAGGTCTGTCTCTCTTCCATGGGACTTGACCGATCTGCAGTCAGCCGCGTTCTCGATATGCACCCCAAACTCCTGACCTCGAATCCGGACTATGATATTTACCCCATCTTTGATTTTCTTCTTAATGAAGTTGAAATTCCATTTCCCGACATTCGTAAATCTATAATTCGATGCCCTAGAATTCTCGTCTCCGACTTGGACCATCAATTGCGACCTGCTTTGAAGTTCTTGCGTGACCTGGGTTTTGTTGGGTTGAAAGCCATCACGTGCCAGACGACATTGCTACTGGTTTCGAGTGTTGAACATACCCTTTTGCCTAAGATTCAGTACCTTGAGAGCTTGGGCTTATCGCATGAGGATGTGGTTAATATGGTGTTAAGGTCTCCGGGTTTGTTGACTTATAGCATTCAAAACAATTTGGTTCCTAAAGTGAGTTATTTCTTAGGAGACATGAAGGGTGATTTGTTGGAATTGAAACGGTTTCCGCAATATTTTTCGTTTAACTTGGAGAGGAAGATTAAGCTGCGTCATCGTTCACTGGTTGAACATGGTTTGTCACTTCCTTTGTCGAAAATGCTGAAGGCTAGTGACGGGGAGTTCAATGCATGGTTGATAGAAATGCGGTGCAGTTCGTTGATGGCTGATCGTTGAAGAACTGTGGTGGAAATGATTCTACAGCTCGATCGACAAGACCAGGCATTCTACCATATTCATCTTTTAATGCTGACCTTGACTATCTAAATTGGTTGGGCTGGAGGGATTCACACGATTGTTCCCATCTTGAGTGAGATCATCAGTCAGAATGTTTGCTATTTAAACAGAGAATACGTCGAAGACAGAGACTCAACGCTTCCACAATTACCATTTGTTGATGCAATGCCGGCCCTGACTACTGTAATTTGTTGAGAACTTTGGAGACTTGCAAGTTTGGCCCCCATCCCAAATGACTGCATCACATCTGGAAGATTGCTGTCTAAGTAGAAAATATCGTGAAAACTATGCGATTCCCCTTGAACCCCTACCATCAATTGAATTTAATTTCCTATGAGAGGCATTGTAAGAACACTCAACCTGTATATTCTAAATCTTTGACCATTGTTTGAAATTCATCAGAAAGAACATGTGGTTGGGTTTGCAAAAGGCTCAAACTTCAAGGAAGAAGAAAGGGAAAGATAGGGTGAAGTGAACCCCCAAAAGGCCCAAATCATATGATAACTTAAGGCAATGTACACCTCCATTCTGGGGGCTATAAATGCAGTCTACTGAAATCATCAGTAGGACTTGAATTATATTTTTCTGGTGGAAGAAAAGTTAGTGGTGTTGAAAATGAAACTAGATTTTGTCCCACCAAAGTTAGAAACAATGAGAGATTGATTTCATAATGCATGATGATTCATTTTCTTGTTGAAACATGAGCCGAAGGACACGGTTTCTTGCTGCAGAAATGCATCACTGTTCAGTAGTCCAGATTCCAGGGGTACGTTGTCCATTACATTACATGACAAGTTTTGAAGGGTACAGTATGTATGAAAGGCCTTGGGGTCATCTCTGTTTCTCGGGACAACAAATGCAAATGCAAATGCAAAATTTGGGGTCAGCATTCGTCACCTCTGGACCCCTTTACCTTCCTACAATCAATTTGCTCAGACACAGAAAAACAAGAGAGAAGGCATCTGTCCCATTCTTTAATCATTCACCCTCGAAGGATGGGATGAGTTTTTGATTTGAAATTCATGGTCTTCATTTAATTCTGTCTCTCTCTGCTCTACTCTACTCTTAAACAGTATTCTATTTATATTATGGTTGGTTGCTTCTAAGACAGATCTGTCTCAGAGGAGAATGAATGTTCTACGTAAGTCAAGATGGTTTCAACTTTCAATACTTGGATTAATTTTGGTTTATACATTAGATGTTAAGATAGAAATGGTTTTTGATTTGTTGGCATGAGATATTATTGTAAATGCTTATCTCGGATGGAAGAGTAGGCCAAAGAGAATTCATAGACAGTGAAATTATCTGTTATGAGACAGCAATTTCAGAATCCACATGTTATGAATTTATGGTTGT

Coding sequence (CDS)

ATGATAAGCAGACTCCCACTTCCTCCCTCAAGCTTCTTCTTCTTCTTCTTTCCTCCCTCATTGGCCGGGTCTCTTAAACCTTTGACAAACCCTAGTTTCAGAAAACCTCGTTTCACCCTTTCTCTCGATACCCAATTTTCTGACCGCTTCGACTCCGGCCTACTTTTCCGTGAGAAAATACTCTACCTTGAGAACCACCTCAATGTCGATTCTCGCAAAGCCTTCCGGGAGAATCCCCATTGTCGCTCTGCTACTCTCTCCACTCTCAAATCCGTCGAGGTCTGTCTCTCTTCCATGGGACTTGACCGATCTGCAGTCAGCCGCGTTCTCGATATGCACCCCAAACTCCTGACCTCGAATCCGGACTATGATATTTACCCCATCTTTGATTTTCTTCTTAATGAAGTTGAAATTCCATTTCCCGACATTCGTAAATCTATAATTCGATGCCCTAGAATTCTCGTCTCCGACTTGGACCATCAATTGCGACCTGCTTTGAAGTTCTTGCGTGACCTGGGTTTTGTTGGGTTGAAAGCCATCACGTGCCAGACGACATTGCTACTGGTTTCGAGTGTTGAACATACCCTTTTGCCTAAGATTCAGTACCTTGAGAGCTTGGGCTTATCGCATGAGGATGTGGTTAATATGGTGTTAAGGTCTCCGGGTTTGTTGACTTATAGCATTCAAAACAATTTGGTTCCTAAAGTGAGTTATTTCTTAGGAGACATGAAGGGTGATTTGTTGGAATTGAAACGGTTTCCGCAATATTTTTCGTTTAACTTGGAGAGGAAGATTAAGCTGCGTCATCGTTCACTGGTTGAACATGGTTTGTCACTTCCTTTGTCGAAAATGCTGAAGGCTAGTGACGGGGAGTTCAATGCATGGTTGATAGAAATGCGGTGCAGTTCGTTGATGGCTGATCGTTGA

Protein sequence

MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSNPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMRCSSLMADR*

Homology

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BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 309.3 bits (791), Expect = 4.9e-83
Identity = 157/254 (61.81%), Postives = 198/254 (77.95%), Query Frame = 0


Query: 51  DSGLLFREKILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVL 110
           D+G+LFREK++YL++ LNVD  KA R NP  RSA +S++ SVE  LSS GL R AV R+L
Sbjct: 28  DTGILFREKLIYLQD-LNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRIL 87

Query: 111 DMHPKLLTSNPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLR 170
           DM P LLTS+P+ +I P+  FL NE+ I   DI KSI RCPR+L+S +D+QLRPAL FL+
Sbjct: 88  DMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLK 147

Query: 171 DLGFVGLKAITCQTTLLLVSSVEHTLLPKIQYL-ESLGLSHEDVVNMVLRSPGLLTYSIQ 230
            LGFVG   IT + T+LLVS+VE TL+PKI+YL E LG + E+V  MV+RSP LLTYS+ 
Sbjct: 148 TLGFVGRDTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVD 207

Query: 231 NNLVPKVSYFLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASD 290
           NNLVPKV +F+ +M+GD+ ELKRFPQYFSF+LERKIK RHR L EHG+ +PLS+MLK SD
Sbjct: 208 NNLVPKVEFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSD 267

Query: 291 GEFNAWLIEMRCSS 304
           G+FN WL+E+R  S
Sbjct: 268 GQFNHWLLELRLRS 280

[Close]

BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.0e-16
Identity = 56/209 (26.79%), Postives = 109/209 (52.15%), Query Frame = 0


Query: 70  DSRKAFRENPHCRSATL-STLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSNPDYDIYPI 129
           D ++     P     T+ + LK+    L  +G+  S + +++   P L + + +  + P 
Sbjct: 235 DIKRMLLRQPQILQYTVENNLKAHISFLMGLGIPNSKIGQIVAATPSLFSYSVENSLRPT 294

Query: 130 FDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFL-RDLGFV--GLKAITCQTT 189
             +L+ EV I   D+ K +   P+ILV  LD        FL ++LG     +  +  +  
Sbjct: 295 IRYLIEEVGIKETDVGKVVQLSPQILVQRLDITWNTRYMFLSKELGAPRDSVVKMVKKHP 354

Query: 190 LLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFLGDMKG 249
            LL  S++   LP+I +L S+G+ + D++ ++     +L+ S+++NL PK  Y + ++  
Sbjct: 355 QLLHYSIDDGFLPRINFLRSIGMCNSDILKVLTSLTQVLSLSLEDNLKPKYMYLVNELNN 414

Query: 250 DLLELKRFPQYFSFNLERKIKLRHRSLVE 275
           ++  L ++P Y S +L+++I+ RHR LVE
Sbjct: 415 EVHILTKYPMYLSLSLDQRIRPRHRFLVE 443

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BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.5e-15
Identity = 58/213 (27.23%), Postives = 109/213 (51.17%), Query Frame = 0


Query: 81  CRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSNPDYDIYPIFDFLLNEVEIPF 140
           C  +    LK     L ++G+D++  ++++   P +LT +    +    +F L++  +  
Sbjct: 260 CGISLTDNLKPTMAFLETLGIDKNQWAKIISRFPAILTYSRQ-KLTSTVEF-LSQTGLTE 319

Query: 141 PDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGF-VGLKAITCQTTLLLVSSVEHTLLPK 200
             I + + RCP I+   ++ +LRP +++ R L   V +    C  T  L  S+E  L P 
Sbjct: 320 EQIGRILTRCPNIMSYSVEDKLRPTMEYFRSLNVDVAVLLHRCPQTFGL--SIESNLKPV 379

Query: 201 IQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFLGDMKGDLLELKRFPQYFSF 260
            ++    G   +++  M+ R   L T+S++ N++PK  YF   M     EL +FPQ+F +
Sbjct: 380 TEFFLEKGFGLDEIGIMISRYGALYTFSLKENVMPKWDYF-QTMDYPKSELVKFPQFFGY 439

Query: 261 NLERKIKLRHRSLVEHGLSLPLSKMLKASDGEF 293
           +L+ +IK R+  +   G+ L L+++L  S  EF
Sbjct: 440 SLQERIKPRYELVQRSGVRLLLNQVLSLSGIEF 467

[Close]

BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.6e-15
Identity = 62/197 (31.47%), Postives = 100/197 (50.76%), Query Frame = 0


Query: 99  MGLDRSAVSRVLDMHPKLLTSNPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDL 158
           +G+ +  + R+L + P L   + +  I P   F L E+ IP   I   +++ P +L + L
Sbjct: 398 LGIPKEGMKRILVVKPILYCIDLEKTIAPKVRF-LQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 159 DHQLRPALKFLRDLGFVGLK---AITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVN 218
             ++RP + FL     V  K    +      LL  S+   L P ++Y  SLG+    +  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 219 MVLRSPGLLTYSIQNNLVPKVSYFLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEH 278
           M+   P LL Y++ +NL PK  Y    M   L +L  FP++FS++LER+I  RH  +VE+
Sbjct: 518 MIADFPMLLRYNV-DNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVEN 577

Query: 279 GLSLPLSKMLKASDGEF 293
            ++  L  ML  +D EF
Sbjct: 578 RVNFKLRYMLACTDEEF 592

[Close]

BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 60/218 (27.52%), Postives = 100/218 (45.87%), Query Frame = 0


Query: 89  LKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSNPDYDIYP----IFDFLLNEVEIPFPDIR 148
           +K +   L  +G+ R A +R+++  P +L    D  + P    + DF + E  +P     
Sbjct: 287 IKPLVEYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLP----- 346

Query: 149 KSIIRCPRILVSDLDHQLRPALKFL--------RDLGFVGLKAITCQTTLLLVSSVEHTL 208
             I + P I+  DL  +L    K L         DLG +       +     VS  E  +
Sbjct: 347 SIIAQYPEIIGIDLKPKLDTQRKLLCSAIHLNPEDLGSL------IERMPQFVSLSESPM 406

Query: 209 LPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVS--YFLGDMKGDLLELKRFP 268
           L  I +L   G S +    MV+  P +L  ++    + K+S  YF  +MK  L +L  FP
Sbjct: 407 LKHIDFLTKCGFSIDQTREMVIGCPQVLALNLG---IMKLSFEYFQKEMKRPLQDLVDFP 466

Query: 269 QYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEF 293
            +F++ LE  +K RH+ +++ G+   L+ ML  SD +F
Sbjct: 467 AFFTYGLESTVKPRHKKIIKKGIKCSLAWMLNCSDEKF 490

[Close]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL
Match: A0A0A0KW02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605740 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.0e-171
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI
Sbjct: 32  MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 91

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN
Sbjct: 92  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 151

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI
Sbjct: 152 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 211

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL
Sbjct: 212 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 271

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
           GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR
Sbjct: 272 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 331

Query: 301 CSSLMADR 309
           CSSLMADR
Sbjct: 332 CSSLMADR 339

[Close]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL
Match: A0A5D3D1U5 (Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00330 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.8e-158
Identity = 287/308 (93.18%), Postives = 294/308 (95.45%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISRL LPP S F FFFPPSLAGSLKPL NPSF KPRFTLSL+TQFSDR DSGLLFREKI
Sbjct: 1   MISRLQLPPPSCFIFFFPPSLAGSLKPLKNPSFGKPRFTLSLNTQFSDRSDSGLLFREKI 60

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVDSRKAFRENPHCRSA+LSTLKSVEVCLSSMGLDRSAV RVLDM+PKLLTSN
Sbjct: 61  LYLENHLNVDSRKAFRENPHCRSASLSTLKSVEVCLSSMGLDRSAVGRVLDMYPKLLTSN 120

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYDIYPIFDFLLNEV+IPFPDI KSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI
Sbjct: 121 PDYDIYPIFDFLLNEVQIPFPDIHKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTTLLLVS+VEHTLLPKIQ+LESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKV+YFL
Sbjct: 181 TCQTTLLLVSNVEHTLLPKIQFLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVNYFL 240

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
           GDMKGDLLELK FPQYFSFNLERKIK RHRSLVEHGLSLPLSKMLK SDGEFNAWL EMR
Sbjct: 241 GDMKGDLLELKSFPQYFSFNLERKIKPRHRSLVEHGLSLPLSKMLKVSDGEFNAWLTEMR 300

Query: 301 CSSLMADR 309
           C SLMADR
Sbjct: 301 CRSLMADR 308

[Close]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL
Match: A0A1S3BFK9 (transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489133 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.8e-158
Identity = 287/308 (93.18%), Postives = 294/308 (95.45%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISRL LPP S F FFFPPSLAGSLKPL NPSF KPRFTLSL+TQFSDR DSGLLFREKI
Sbjct: 1   MISRLQLPPPSCFIFFFPPSLAGSLKPLKNPSFGKPRFTLSLNTQFSDRSDSGLLFREKI 60

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVDSRKAFRENPHCRSA+LSTLKSVEVCLSSMGLDRSAV RVLDM+PKLLTSN
Sbjct: 61  LYLENHLNVDSRKAFRENPHCRSASLSTLKSVEVCLSSMGLDRSAVGRVLDMYPKLLTSN 120

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYDIYPIFDFLLNEV+IPFPDI KSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI
Sbjct: 121 PDYDIYPIFDFLLNEVQIPFPDIHKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTTLLLVS+VEHTLLPKIQ+LESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKV+YFL
Sbjct: 181 TCQTTLLLVSNVEHTLLPKIQFLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVNYFL 240

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
           GDMKGDLLELK FPQYFSFNLERKIK RHRSLVEHGLSLPLSKMLK SDGEFNAWL EMR
Sbjct: 241 GDMKGDLLELKSFPQYFSFNLERKIKPRHRSLVEHGLSLPLSKMLKVSDGEFNAWLTEMR 300

Query: 301 CSSLMADR 309
           C SLMADR
Sbjct: 301 CRSLMADR 308

[Close]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL
Match: A0A6J1G0M2 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449597 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 8.6e-131
Identity = 249/308 (80.84%), Postives = 272/308 (88.31%), Query Frame = 0


Query: 1   MISRLP-LPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREK 60
           M+SR+  LPP  +  F FP S A SL PL NP+FRKPRFT+SL++QFSD  D+GLLFR+K
Sbjct: 1   MMSRVQNLPP--WNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDHSDAGLLFRQK 60

Query: 61  ILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTS 120
           ILYLENHLNVD RKAFRENP  RSATLS +KSVEVCLSSMGL+ SAV RVLDM+P+LLTS
Sbjct: 61  ILYLENHLNVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQLLTS 120

Query: 121 NPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKA 180
           +PDYD+YPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLD QLRPALKFLRDLGFVGL A
Sbjct: 121 DPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFVGLNA 180

Query: 181 ITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYF 240
           IT QTTLLLVSSVEHTLLPKIQYLE+LGLSH+DVVNMVLRSP LLTYSIQNNLVPKV YF
Sbjct: 181 ITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPKVDYF 240

Query: 241 LGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEM 300
           LGDMKGDL EL+RFPQYFSF+LERKIK RHR LVEHGLSL LSKMLK SDGEF A L+EM
Sbjct: 241 LGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTARLLEM 300

Query: 301 RCSSLMAD 308
           R  S  AD
Sbjct: 301 RLQSSTAD 306

[Close]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL
Match: A0A6J1HTW2 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467425 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.6e-129
Identity = 246/308 (79.87%), Postives = 270/308 (87.66%), Query Frame = 0


Query: 1   MISRLP-LPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREK 60
           MISR+  LPP +   F FP S   SL P+ NP+FRKPRFT+SL++QFSD  D+GL FR+K
Sbjct: 1   MISRVQNLPPCN--RFLFPRSSTPSLNPMRNPNFRKPRFTISLNSQFSDHSDAGLHFRQK 60

Query: 61  ILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTS 120
           ILYLENHL+VD RKAFRENP  RSATLS +KSVEVCLSSMGL+ SAV RVLDM+P+LLTS
Sbjct: 61  ILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQLLTS 120

Query: 121 NPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKA 180
           +PDYD+YPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLD QLRPALKFLRDLGFVGL A
Sbjct: 121 DPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFVGLNA 180

Query: 181 ITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYF 240
           IT QTTLLLVSSVEHTLLPKIQYLE+LGLSH+DVVNMVLRSP LLTYSIQNNLVPKV YF
Sbjct: 181 ITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPKVDYF 240

Query: 241 LGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEM 300
           LGDMKGDL EL+RFPQYFSF+LERKIK RHR LVEHGLSL LSKMLK SDGEF A L+EM
Sbjct: 241 LGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTARLLEM 300

Query: 301 RCSSLMAD 308
           R  S  AD
Sbjct: 301 RLQSSTAD 306

[Close]

BLAST of CSPI05G23850 vs. NCBI nr
Match: XP_004135172.1 (transcription termination factor MTEF1, chloroplastic [Cucumis sativus] >KAE8648749.1 hypothetical protein Csa_007991 [Cucumis sativus])

HSP 1 Score: 610.1 bits (1572), Expect = 1.0e-170
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI
Sbjct: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN
Sbjct: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI
Sbjct: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL
Sbjct: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
           GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR
Sbjct: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300

Query: 301 CSSLMADR 309
           CSSLMADR
Sbjct: 301 CSSLMADR 308

[Close]

BLAST of CSPI05G23850 vs. NCBI nr
Match: XP_008446371.1 (PREDICTED: transcription termination factor MTEF1, chloroplastic [Cucumis melo] >KAA0034429.1 transcription termination factor MTEF1 [Cucumis melo var. makuwa] >TYK17705.1 transcription termination factor MTEF1 [Cucumis melo var. makuwa])

HSP 1 Score: 567.0 bits (1460), Expect = 9.9e-158
Identity = 287/308 (93.18%), Postives = 294/308 (95.45%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISRL LPP S F FFFPPSLAGSLKPL NPSF KPRFTLSL+TQFSDR DSGLLFREKI
Sbjct: 1   MISRLQLPPPSCFIFFFPPSLAGSLKPLKNPSFGKPRFTLSLNTQFSDRSDSGLLFREKI 60

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVDSRKAFRENPHCRSA+LSTLKSVEVCLSSMGLDRSAV RVLDM+PKLLTSN
Sbjct: 61  LYLENHLNVDSRKAFRENPHCRSASLSTLKSVEVCLSSMGLDRSAVGRVLDMYPKLLTSN 120

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYDIYPIFDFLLNEV+IPFPDI KSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI
Sbjct: 121 PDYDIYPIFDFLLNEVQIPFPDIHKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTTLLLVS+VEHTLLPKIQ+LESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKV+YFL
Sbjct: 181 TCQTTLLLVSNVEHTLLPKIQFLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVNYFL 240

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
           GDMKGDLLELK FPQYFSFNLERKIK RHRSLVEHGLSLPLSKMLK SDGEFNAWL EMR
Sbjct: 241 GDMKGDLLELKSFPQYFSFNLERKIKPRHRSLVEHGLSLPLSKMLKVSDGEFNAWLTEMR 300

Query: 301 CSSLMADR 309
           C SLMADR
Sbjct: 301 CRSLMADR 308

[Close]

BLAST of CSPI05G23850 vs. NCBI nr
Match: XP_038892906.1 (transcription termination factor MTEF1, chloroplastic [Benincasa hispida] >XP_038892907.1 transcription termination factor MTEF1, chloroplastic [Benincasa hispida])

HSP 1 Score: 496.5 bits (1277), Expect = 1.7e-136
Identity = 260/308 (84.42%), Postives = 274/308 (88.96%), Query Frame = 0


Query: 1   MISRLPLPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREKI 60
           MISR  LPP     F FP S   +LKPL NP+FRKPRF LSL+TQFSD  D+GLLFREKI
Sbjct: 1   MISRFQLPPPC---FLFPRSSFQALKPLRNPNFRKPRFALSLNTQFSDHSDAGLLFREKI 60

Query: 61  LYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSN 120
           LYLENHLNVD RKAFRENP  RSATLST+KSVEVCLSSMGL+ SAV RVLDM+PKLLT+N
Sbjct: 61  LYLENHLNVDFRKAFRENPDSRSATLSTVKSVEVCLSSMGLELSAVGRVLDMYPKLLTAN 120

Query: 121 PDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAI 180
           PDYD+YPIFDFLLNEV+IPFPDIRKSIIRCPRILVSDLD QLRPALKFLRDLGFVGL AI
Sbjct: 121 PDYDLYPIFDFLLNEVQIPFPDIRKSIIRCPRILVSDLDLQLRPALKFLRDLGFVGLNAI 180

Query: 181 TCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFL 240
           TCQTT+LLVSSVE TLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLV KV+YFL
Sbjct: 181 TCQTTMLLVSSVERTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVRKVNYFL 240

Query: 241 GDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMR 300
            DMKGDLLELK FPQYFSF+LERKIK RHRSLV+HGLSLPLSKMLK SDGEF A LIEMR
Sbjct: 241 EDMKGDLLELKIFPQYFSFSLERKIKPRHRSLVQHGLSLPLSKMLKVSDGEFTARLIEMR 300

Query: 301 CSSLMADR 309
             SL ADR
Sbjct: 301 LQSLTADR 305

[Close]

BLAST of CSPI05G23850 vs. NCBI nr
Match: XP_022945324.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita moschata] >XP_022945325.1 transcription termination factor MTEF1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 476.5 bits (1225), Expect = 1.8e-130
Identity = 249/308 (80.84%), Postives = 272/308 (88.31%), Query Frame = 0


Query: 1   MISRLP-LPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREK 60
           M+SR+  LPP  +  F FP S A SL PL NP+FRKPRFT+SL++QFSD  D+GLLFR+K
Sbjct: 1   MMSRVQNLPP--WNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDHSDAGLLFRQK 60

Query: 61  ILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTS 120
           ILYLENHLNVD RKAFRENP  RSATLS +KSVEVCLSSMGL+ SAV RVLDM+P+LLTS
Sbjct: 61  ILYLENHLNVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQLLTS 120

Query: 121 NPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKA 180
           +PDYD+YPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLD QLRPALKFLRDLGFVGL A
Sbjct: 121 DPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFVGLNA 180

Query: 181 ITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYF 240
           IT QTTLLLVSSVEHTLLPKIQYLE+LGLSH+DVVNMVLRSP LLTYSIQNNLVPKV YF
Sbjct: 181 ITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPKVDYF 240

Query: 241 LGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEM 300
           LGDMKGDL EL+RFPQYFSF+LERKIK RHR LVEHGLSL LSKMLK SDGEF A L+EM
Sbjct: 241 LGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTARLLEM 300

Query: 301 RCSSLMAD 308
           R  S  AD
Sbjct: 301 RLQSSTAD 306

[Close]

BLAST of CSPI05G23850 vs. NCBI nr
Match: KAG7013135.1 (Transcription termination factor MTEF1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 475.7 bits (1223), Expect = 3.0e-130
Identity = 249/308 (80.84%), Postives = 272/308 (88.31%), Query Frame = 0


Query: 1   MISRLP-LPPSSFFFFFFPPSLAGSLKPLTNPSFRKPRFTLSLDTQFSDRFDSGLLFREK 60
           MISR+  LPP  +  F FP S A SL PL NP+FRKPRFT+SL++QFSD  D+GLLFR+K
Sbjct: 1   MISRVQNLPP--WNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDHSDAGLLFRQK 60

Query: 61  ILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTS 120
           ILYLENHL+VD RKAFRENP  RSATLS +KSVEVCLSSMGL+ SAV RVLDM+P+LLTS
Sbjct: 61  ILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQLLTS 120

Query: 121 NPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKA 180
           +PDYD+YPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLD QLRPALKFLRDLGFVGL A
Sbjct: 121 DPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFVGLNA 180

Query: 181 ITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYF 240
           IT QTTLLLVSSVEHTLLPKIQYLE+LGLSH+DVVNMVLRSP LLTYSIQNNLVPKV YF
Sbjct: 181 ITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPKVDYF 240

Query: 241 LGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEM 300
           LGDMKGDL EL+RFPQYFSF+LERKIK RHR LVEHGLSL LSKMLK SDGEF A L+EM
Sbjct: 241 LGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTARLLEM 300

Query: 301 RCSSLMAD 308
           R  S  AD
Sbjct: 301 RLQSSTAD 306

[Close]

BLAST of CSPI05G23850 vs. TAIR 10
Match: AT2G03050.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.5e-84
Identity = 157/254 (61.81%), Postives = 198/254 (77.95%), Query Frame = 0


Query: 51  DSGLLFREKILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVL 110
           D+G+LFREK++YL++ LNVD  KA R NP  RSA +S++ SVE  LSS GL R AV R+L
Sbjct: 27  DTGILFREKLIYLQD-LNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRIL 86

Query: 111 DMHPKLLTSNPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLR 170
           DM P LLTS+P+ +I P+  FL NE+ I   DI KSI RCPR+L+S +D+QLRPAL FL+
Sbjct: 87  DMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLK 146

Query: 171 DLGFVGLKAITCQTTLLLVSSVEHTLLPKIQYL-ESLGLSHEDVVNMVLRSPGLLTYSIQ 230
            LGFVG   IT + T+LLVS+VE TL+PKI+YL E LG + E+V  MV+RSP LLTYS+ 
Sbjct: 147 TLGFVGRDTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVD 206

Query: 231 NNLVPKVSYFLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASD 290
           NNLVPKV +F+ +M+GD+ ELKRFPQYFSF+LERKIK RHR L EHG+ +PLS+MLK SD
Sbjct: 207 NNLVPKVEFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSD 266

Query: 291 GEFNAWLIEMRCSS 304
           G+FN WL+E+R  S
Sbjct: 267 GQFNHWLLELRLRS 279

[Close]

BLAST of CSPI05G23850 vs. TAIR 10
Match: AT2G34620.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-57
Identity = 116/243 (47.74%), Postives = 167/243 (68.72%), Query Frame = 0


Query: 54  LLFREKILYLENHLNVDSRKAFRENPHCRSATLSTLKSVEVCLSSMGLDRSAVSRVLDMH 113
           L  +EKIL LE  + +DS KA   NP   SA L +++SV   L S G+  + + R+L M 
Sbjct: 53  LQMKEKILCLE-LMGIDSGKALSLNPCLCSAPLDSIQSVLHFLQSKGIYPNDLPRILGMC 112

Query: 114 PKLLTSNPDYDIYPIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLG 173
           PK+LTS+   ++YP+F FL N++ +P    R+ I +CPR+L+S ++ QL+PAL +L+ LG
Sbjct: 113 PKILTSDVRTELYPVFMFLSNDLHVPENAFRRVIKKCPRLLISSVEDQLKPALFYLQRLG 172

Query: 174 FVGLKAITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLV 233
              L+A+  Q  +LLVSSVEHTL+PK+++LES+G S  + + M+LR P L T+SI+NN  
Sbjct: 173 LKDLEALAYQDPILLVSSVEHTLIPKLRFLESIGFSRPEAIGMILRCPALFTFSIENNFK 232

Query: 234 PKVSYFLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFN 293
           PK+ YF+ ++KG L  LK FPQYF+F+LE++IK RH   +E GL LPLS MLK++D EF 
Sbjct: 233 PKLDYFMSEIKGKLENLKEFPQYFAFSLEKRIKPRHLESMERGLELPLSLMLKSTDEEFE 292

Query: 294 AWL 297
             L
Sbjct: 293 QLL 294

[Close]

BLAST of CSPI05G23850 vs. TAIR 10
Match: AT2G36000.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 145.2 bits (365), Expect = 8.7e-35
Identity = 101/291 (34.71%), Postives = 152/291 (52.23%), Query Frame = 0


Query: 24  SLKPLTNPS----FRKPRF----TLSLDTQFSDRFDSGLLFREKILYLENHLNVDSRKAF 83
           S KP T+ S    F+ P      + +  T  ++  +S +   EK++YL++ L +D     
Sbjct: 35  SFKPNTSSSSSSFFKSPDIPSLSSTTTTTTTTETLESSI--HEKLIYLDS-LGIDFLTLI 94

Query: 84  RENPHCRSATLSTLKSVEVCLSS--MGLDRSAVSRVLDMHPKLLTSNPDYDIYPIFDFLL 143
             +P   S  LS ++SV   +++  +        R++ M P+LLTS       P+  FLL
Sbjct: 95  NRHPPLLSTALSAVESVVDYMTTPPINFTLQDFRRLVSMCPELLTSPLTSHTIPVITFLL 154

Query: 144 NEVEI-PFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAITCQTTLLLVSSV 203
            EV +    D+R+++ R PR+L   +DHQLRP L FL+ +G +       + T LL  SV
Sbjct: 155 REVGVDSIFDLRQALRRRPRLLACSVDHQLRPTLYFLQRIGILD----PHKHTYLLSCSV 214

Query: 204 EHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFLGDMKGDLLELKR 263
           ++ L+P+I Y E LG S      M  R P L  YSI  N  PK+ Y + +M  D+ E+  
Sbjct: 215 DNKLVPRIDYFEKLGFSRRSATAMFKRFPQLFNYSIAENYEPKLKYLMVEMGRDVREVLE 274

Query: 264 FPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEFNAWLIEMRCSS 304
           FPQYFSF+LE +IK RH +    G+  PL  MLK ++  F   L E+ C S
Sbjct: 275 FPQYFSFSLENRIKPRHEACAAKGVRFPLPVMLKTNEAGFRDTL-EVCCDS 317

[Close]

BLAST of CSPI05G23850 vs. TAIR 10
Match: AT2G36000.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 143.3 bits (360), Expect = 3.3e-34
Identity = 97/280 (34.64%), Postives = 147/280 (52.50%), Query Frame = 0


Query: 24  SLKPLTNPS----FRKPRF----TLSLDTQFSDRFDSGLLFREKILYLENHLNVDSRKAF 83
           S KP T+ S    F+ P      + +  T  ++  +S +   EK++YL++ L +D     
Sbjct: 35  SFKPNTSSSSSSFFKSPDIPSLSSTTTTTTTTETLESSI--HEKLIYLDS-LGIDFLTLI 94

Query: 84  RENPHCRSATLSTLKSVEVCLSS--MGLDRSAVSRVLDMHPKLLTSNPDYDIYPIFDFLL 143
             +P   S  LS ++SV   +++  +        R++ M P+LLTS       P+  FLL
Sbjct: 95  NRHPPLLSTALSAVESVVDYMTTPPINFTLQDFRRLVSMCPELLTSPLTSHTIPVITFLL 154

Query: 144 NEVEI-PFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLKAITCQTTLLLVSSV 203
            EV +    D+R+++ R PR+L   +DHQLRP L FL+ +G +       + T LL  SV
Sbjct: 155 REVGVDSIFDLRQALRRRPRLLACSVDHQLRPTLYFLQRIGILD----PHKHTYLLSCSV 214

Query: 204 EHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSYFLGDMKGDLLELKR 263
           ++ L+P+I Y E LG S      M  R P L  YSI  N  PK+ Y + +M  D+ E+  
Sbjct: 215 DNKLVPRIDYFEKLGFSRRSATAMFKRFPQLFNYSIAENYEPKLKYLMVEMGRDVREVLE 274

Query: 264 FPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEF 293
           FPQYFSF+LE +IK RH +    G+  PL  MLK ++  F
Sbjct: 275 FPQYFSFSLENRIKPRHEACAAKGVRFPLPVMLKTNEAGF 307

[Close]

BLAST of CSPI05G23850 vs. TAIR 10
Match: AT3G18870.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 126.3 bits (316), Expect = 4.2e-29
Identity = 80/234 (34.19%), Postives = 131/234 (55.98%), Query Frame = 0


Query: 64  ENHLNVDSRKAFRENPHCR--SATLSTLKSVEVCLSSMGLDRSAVSRVLDMHPKLLTSNP 123
           EN  ++ S     +NP     +  LS + S    L S G+      R++ + P+L   +P
Sbjct: 38  ENLRHLSSLGIVPQNPRLAPPANDLSVILSAVNLLKSKGISDEDFPRLVFLCPQLF--SP 97

Query: 124 DYDIY---PIFDFLLNEVEIPFPDIRKSIIRCPRILVSDLDHQLRPALKFLRDLGFVGLK 183
            +DI    P+FDFL  E+     + R  I+ CP IL SD+++ LRP L +L++LG   L 
Sbjct: 98  TFDISKLDPVFDFLTGELGASAEESRGLIVNCPNILFSDVEYCLRPTLVYLKELGVRNLN 157

Query: 184 AITCQTTLLLVSSVEHTLLPKIQYLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVPKVSY 243
             +     +L + VE  L  K+++L+S+G  HE+   +  R P +  YS+++NL PK  +
Sbjct: 158 RASKTNAHVLNTRVE-KLRAKMRFLKSIGFEHEEAARVCGRIPAIFGYSVEDNLRPKFEF 217

Query: 244 FLGDMKGDLLELKRFPQYFSFNLERKIKLRHRSLVEHGLSLPLSKMLKASDGEF 293
            + DM+ +L ELK+FPQYF+F+L ++I+ RH  L +  + + LS+ML   D +F
Sbjct: 218 LVYDMERELEELKKFPQYFAFSLGKRIRPRHWHLKKKNVRVSLSRMLMWGDQKF 268

The following BLAST results are available for this feature:

BLAST of CSPI05G23850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84X53	4.9e-83	61.81	Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX...	[more]
Q9FM80	3.0e-16	26.79	Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O...	[more]
F4JVI3	1.5e-15	27.23	Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O...	[more]
F4IHL3	2.6e-15	31.47	Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O...	[more]
Q9ZT96	1.1e-10	27.52	Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of CSPI05G23850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW02	5.0e-171	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605740 PE=3 SV=1	[more]
A0A5D3D1U5	4.8e-158	93.18	Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BFK9	4.8e-158	93.18	transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1G0M2	8.6e-131	80.84	transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3...	[more]
A0A6J1HTW2	3.6e-129	79.87	transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=366...	[more]

BLAST of CSPI05G23850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135172.1	1.0e-170	100.00	transcription termination factor MTEF1, chloroplastic [Cucumis sativus] >KAE8648...	[more]
XP_008446371.1	9.9e-158	93.18	PREDICTED: transcription termination factor MTEF1, chloroplastic [Cucumis melo] ...	[more]
XP_038892906.1	1.7e-136	84.42	transcription termination factor MTEF1, chloroplastic [Benincasa hispida] >XP_03...	[more]
XP_022945324.1	1.8e-130	80.84	transcription termination factor MTEF1, chloroplastic [Cucurbita moschata] >XP_0...	[more]
KAG7013135.1	3.0e-130	80.84	Transcription termination factor MTEF1, chloroplastic [Cucurbita argyrosperma su...	[more]

BLAST of CSPI05G23850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03050.1	3.5e-84	61.81	Mitochondrial transcription termination factor family protein	[more]
AT2G34620.1	1.2e-57	47.74	Mitochondrial transcription termination factor family protein	[more]
AT2G36000.1	8.7e-35	34.71	Mitochondrial transcription termination factor family protein	[more]
AT2G36000.2	3.3e-34	34.64	Mitochondrial transcription termination factor family protein	[more]
AT3G18870.1	4.2e-29	34.19	Mitochondrial transcription termination factor family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003690	Transcription termination factor, mitochondrial/chloroplastic	SMART	SM00733	mt_12	coord: 72..102 e-value: 2500.0 score: 0.8 coord: 144..175 e-value: 2.9E-4 score: 30.2 coord: 214..246 e-value: 0.038 score: 23.1 coord: 107..139 e-value: 15.0 score: 14.5 coord: 247..278 e-value: 0.008 score: 25.4 coord: 178..209 e-value: 0.35 score: 19.9
IPR003690	Transcription termination factor, mitochondrial/chloroplastic	PFAM	PF02536	mTERF	coord: 70..292 e-value: 2.2E-31 score: 108.9
IPR003690	Transcription termination factor, mitochondrial/chloroplastic	PANTHER	PTHR13068	CGI-12 PROTEIN-RELATED	coord: 37..304
IPR038538	MTERF superfamily, mitochondrial/chloroplastic	GENE3D	1.25.70.10	Transcription termination factor 3, mitochondrial	coord: 67..302 e-value: 1.3E-44 score: 154.3
None	No IPR available	PANTHER	PTHR13068:SF36	TRANSCRIPTION TERMINATION FACTOR MTEF1, CHLOROPLASTIC	coord: 37..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G23850.1	CSPI05G23850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006353	DNA-templated transcription, termination
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003690	double-stranded DNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity