CSPI05G23020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G23020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 4
LocationChr5: 23233841 .. 23236363 (-)
RNA-Seq ExpressionCSPI05G23020
SyntenyCSPI05G23020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTGTTCATGAATGGAGTACAAATAGAAGATTGTTTTTCAGTTTACTACAACGTCTTCTTCTTCCTTACCCATAATGGAGTTTTTGTACATATTATTTTTCATCTCCATTTTTCTCCCTCTAACCTTTTCTCTCAACCTTGAATCTCCTGATCTTCACGGAACCACTTCCATTTCCCTTCCTACCAATCATACTTCTTCACTCACACACCAAGTTATCGACCTCGCCAATCATCCCACCACTGTTAATTGGATGAAGACCATAAGGAGAAAAATTCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAACTTGACAATCTTCGTGTTTCTTATCGATGGCCGGTCGCTGGTACCGGTGTTGTGGCTTTCGTTGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGTTAGATTTTGGATACTTTTATCTTTCTCATTCTAATTCTTCATCCATTAAATGCTAGCTTCTGTTCGATGAAATGACCCATTGAACTTGAATTGTAATTCTCCATCTACTTTCTTGAATTTGGTGTAATCTTCAGGTTGTTCTCTTCAGTTTATTAATCGCATTTTTATCTTATATGACCGACAATAATACTGGATTATGACTGCACCCATGGAAGACTTTCATTCTTGATAAATACGAAATCTAGAAAAGAACTAGCTAAAGAAGTCTACAACATGAAAGAATTGCTAATTTGACAAAAACTCATAAATTACCTCTATGATTTGATAAAGTTCTCATAACTGGTTACGGTTTTATCAAACTATAGAGCGAATGAGAATAAATTCCAACTTTTCACAAGACCATTTTGAGCAAATTTGCGATTTGACATTTTTTAAATTAGGCAAAGACGGTGTCAGATCTACTGGAGTCTACAATCAGCGAGGACCAGCCATTGAGATTATAGATTATTATTATTATTTTTTTAAAAAAAGCTTGTCTTTCTCATTTTAATGTGGAACTGGGCCATATAATGATGTACAGTGTAATGCAGCAAGAGAAAGTAATTGTGAGGTTTTCATACATACAATGAGTGAAAATCTAAACTCAAGAGTTTCGTTTTGAACTCTAAATTGATTTTGATACAGGAATTGGTGGAATGGGAACATAAGAGCAAAGTTGAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAAATTCTGAATCAACTGCGACATAAATTACAGGTTCTCAAAATACATTGCAAGTACTCCCGACTTCCAACAACTTTATGTTTAACTTTTTAAGATGATTATGACTCAATCTTGTATCCTTTTCTTGTTAATATGTAGATTTCCCTTCAATGCAGGGCACTGTTGTTCTTGTATTTCAGCCAGCGGAGGAGAAAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGCGCTTGATGGTGTAGAAGCTATATTTGGACTGCACGTCGTGCATGAATATCCAGTGGGCGTTGTCGCTTCACGCCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCAGCAATTCCACAGGATTCAATTGATCCTATTTTAGCAGCTTCAGCAGCAATTATAAGTTTGCAAAGCATAGTTTCAAGAGAGATAGACCCTTTAGATTCTCAGGTACTTTGTTTCAGATGAACATAAATGAAAAGGTTTTAAATGCGTTCAATTTAGCCTTTTGCAACTCTATGGCCTGATCTGATCATATAAGTGTTGAAAGCTGCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTCAATGTCATCCCGGAGTCCGCGACTATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCCCTAAGAGACAGGATAGAAGAGGTAACACCGACTACAGGGTCTTGTACAGAACAGATGACTTGTCACTGTAATTTCTGACTGAACTTTTATTGTTCATTTATTGTGACAGGTCATTAACGGGCAGGCTGTGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCGACGATAAACGACGAGAAAATATATGAACATGTTCGACATGTCTCTATGGAAATTGTAGGAAAAGAAAAAACAAAAGTATCTCCACGCCTCATGGGAAGTGAAGATTTTGCTTTCTTTGCAGACAAAGTGCCAGGATCTTTCTTATTTCTTGGCACATATAACGAGAGGATCGGAGCTATACATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCAATTGGCGCAGCAATTCATGCAGCAGTGGCATATTCATATCTCTTGAATTCATCATCAACATCTCATTCTTTCTTTGACCACTGAATATTTGTCAGTACATCCTATAACTCAGTTTTTTTGTTTCCCCATATTCTACAAACTTGAATAGGGAATTTTCTTCTACTTATTACAGGAGAGTTTTGC

mRNA sequence

AACTGTTCATGAATGGAGTACAAATAGAAGATTGTTTTTCAGTTTACTACAACGTCTTCTTCTTCCTTACCCATAATGGAGTTTTTGTACATATTATTTTTCATCTCCATTTTTCTCCCTCTAACCTTTTCTCTCAACCTTGAATCTCCTGATCTTCACGGAACCACTTCCATTTCCCTTCCTACCAATCATACTTCTTCACTCACACACCAAGTTATCGACCTCGCCAATCATCCCACCACTGTTAATTGGATGAAGACCATAAGGAGAAAAATTCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAACTTGACAATCTTCGTGTTTCTTATCGATGGCCGGTCGCTGGTACCGGTGTTGTGGCTTTCGTTGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGAATTGGTGGAATGGGAACATAAGAGCAAAGTTGAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAAATTCTGAATCAACTGCGACATAAATTACAGGGCACTGTTGTTCTTGTATTTCAGCCAGCGGAGGAGAAAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGCGCTTGATGGTGTAGAAGCTATATTTGGACTGCACGTCGTGCATGAATATCCAGTGGGCGTTGTCGCTTCACGCCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCAGCAATTCCACAGGATTCAATTGATCCTATTTTAGCAGCTTCAGCAGCAATTATAAGTTTGCAAAGCATAGTTTCAAGAGAGATAGACCCTTTAGATTCTCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTCAATGTCATCCCGGAGTCCGCGACTATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCCCTAAGAGACAGGATAGAAGAGGTCATTAACGGGCAGGCTGTGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCGACGATAAACGACGAGAAAATATATGAACATGTTCGACATGTCTCTATGGAAATTGTAGGAAAAGAAAAAACAAAAGTATCTCCACGCCTCATGGGAAGTGAAGATTTTGCTTTCTTTGCAGACAAAGTGCCAGGATCTTTCTTATTTCTTGGCACATATAACGAGAGGATCGGAGCTATACATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCAATTGGCGCAGCAATTCATGCAGCAGTGGCATATTCATATCTCTTGAATTCATCATCAACATCTCATTCTTTCTTTGACCACTGAATATTTGTCAGTACATCCTATAACTCAGTTTTTTTGTTTCCCCATATTCTACAAACTTGAATAGGGAATTTTCTTCTACTTATTACAGGAGAGTTTTGC

Coding sequence (CDS)

ATGGAGTTTTTGTACATATTATTTTTCATCTCCATTTTTCTCCCTCTAACCTTTTCTCTCAACCTTGAATCTCCTGATCTTCACGGAACCACTTCCATTTCCCTTCCTACCAATCATACTTCTTCACTCACACACCAAGTTATCGACCTCGCCAATCATCCCACCACTGTTAATTGGATGAAGACCATAAGGAGAAAAATTCACGAGAACCCAGAACTTGCTTTTGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAACTTGACAATCTTCGTGTTTCTTATCGATGGCCGGTCGCTGGTACCGGTGTTGTGGCTTTCGTTGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCTGATATGGATGCTTTGCCTATTGAGGAATTGGTGGAATGGGAACATAAGAGCAAAGTTGAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGTGCTACTAAAATTCTGAATCAACTGCGACATAAATTACAGGGCACTGTTGTTCTTGTATTTCAGCCAGCGGAGGAGAAAGGAGGAGGAGCAAAAGACATGATAAATGAAGGTGCGCTTGATGGTGTAGAAGCTATATTTGGACTGCACGTCGTGCATGAATATCCAGTGGGCGTTGTCGCTTCACGCCCCGGCGAGTTTCTTGCAGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCAGCAATTCCACAGGATTCAATTGATCCTATTTTAGCAGCTTCAGCAGCAATTATAAGTTTGCAAAGCATAGTTTCAAGAGAGATAGACCCTTTAGATTCTCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTCAATGTCATCCCGGAGTCCGCGACTATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCCCTAAGAGACAGGATAGAAGAGGTCATTAACGGGCAGGCTGTGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGCAAGGAACACCCAACAATTCCTCCGACGATAAACGACGAGAAAATATATGAACATGTTCGACATGTCTCTATGGAAATTGTAGGAAAAGAAAAAACAAAAGTATCTCCACGCCTCATGGGAAGTGAAGATTTTGCTTTCTTTGCAGACAAAGTGCCAGGATCTTTCTTATTTCTTGGCACATATAACGAGAGGATCGGAGCTATACATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCAATTGGCGCAGCAATTCATGCAGCAGTGGCATATTCATATCTCTTGAATTCATCATCAACATCTCATTCTTTCTTTGACCACTGA

Protein sequence

MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVLPIGAAIHAAVAYSYLLNSSSTSHSFFDH*
Homology
BLAST of CSPI05G23020 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.0e-129
Identity = 234/379 (61.74%), Postives = 285/379 (75.20%), Query Frame = 0

Query: 59  WMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVAL 118
           WM  +RR+IHENPEL +EEF TS L+R+ELD L + YR P A TGVVA VG+G PPFVAL
Sbjct: 48  WMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVATVGTGGPPFVAL 107

Query: 119 RADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQ 178
           RADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R +L+GTVVLVFQ
Sbjct: 108 RADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHRDELKGTVVLVFQ 167

Query: 179 PAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKG 238
           PAEE GGGAK MI++GA++ +EAIFG+HV    P+GVVASRPG  +AG G F+A I GKG
Sbjct: 168 PAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAGSGFFEAVISGKG 227

Query: 239 GHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIA 298
           GHAA+P  +IDPILAAS  I+SLQ +VSRE DPLDSQVV+V   Q G A NVIP+S TI 
Sbjct: 228 GHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGGAFNVIPDSVTIG 287

Query: 299 GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRH 358
           GTFRAF K+SFN L+ RIEEVI  QA V RC A +DFL K+ P  PPTIN   +++    
Sbjct: 288 GTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSAGLHDFFVK 347

Query: 359 VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGS-FLFLGTYNERIGAIHPPHSPRYKIDE 418
           V+ E+VG +  +    LMG+EDFAF+AD +P + + FLG YNE  G   P HSP + I+E
Sbjct: 348 VASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHHSPYFTINE 407

Query: 419 NVLPIGAAIHAAVAYSYLL 437
           + LP GAA+ A++A  YLL
Sbjct: 408 DALPYGAALQASLAARYLL 426

BLAST of CSPI05G23020 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 3.0e-129
Identity = 233/379 (61.48%), Postives = 284/379 (74.93%), Query Frame = 0

Query: 59  WMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVAL 118
           WM  +RR+IHENPEL +EEF TS L+R+ELD L + YR P A TGVVA VG+G PPFVAL
Sbjct: 48  WMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVATVGTGGPPFVAL 107

Query: 119 RADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQ 178
           RADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R +L+GTVVLVFQ
Sbjct: 108 RADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHRDELKGTVVLVFQ 167

Query: 179 PAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKG 238
           PAEE GGGAK MI++G ++ +EAIFG+HV    P+GVVASRPG  +AG G F+A I GKG
Sbjct: 168 PAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAGSGFFEAVISGKG 227

Query: 239 GHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIA 298
           GHAA+P  +IDPILAAS  I+SLQ +VSRE DPLDSQVV+V   Q G A NVIP+S TI 
Sbjct: 228 GHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGGAFNVIPDSVTIG 287

Query: 299 GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRH 358
           GTFRAF K+SFN L+ RIEEVI  QA V RC A +DFL K+ P  PPTIN   +++    
Sbjct: 288 GTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSAGLHDFFVK 347

Query: 359 VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGS-FLFLGTYNERIGAIHPPHSPRYKIDE 418
           V+ E+VG +  +    LMG+EDFAF+AD +P + + FLG YNE  G   P HSP + I+E
Sbjct: 348 VASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHHSPYFTINE 407

Query: 419 NVLPIGAAIHAAVAYSYLL 437
           + LP GAA+ A++A  YLL
Sbjct: 408 DALPYGAALQASLATRYLL 426

BLAST of CSPI05G23020 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 458.8 bits (1179), Expect = 7.3e-128
Identity = 236/433 (54.50%), Postives = 308/433 (71.13%), Query Frame = 0

Query: 10  ISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWMKTIRRKIHE 69
           +S F  ++F L L    L   T IS  +N  S +  + + LA      +WM  IRR+IHE
Sbjct: 1   MSFFKWVSFVLILH---LLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHE 60

Query: 70  NPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEE 129
           NPEL +EE ETS+L+R EL+ + VSY++PVA TGVV +VG+G  PFVALRADMDAL ++E
Sbjct: 61  NPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQE 120

Query: 130 LVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPAEEKGGGAKD 189
           +VEWEHKSKV GKMHAC HDAH  MLLGA K+L +   +LQGTVVLVFQPAEE GGGAK 
Sbjct: 121 MVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKK 180

Query: 190 MINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQDSID 249
           ++  G L+ V AIFGLHV ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQ +ID
Sbjct: 181 IVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTID 240

Query: 250 PILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGTFRAFSKKSF 309
           PILAAS  I+SLQ +VSRE DPLDSQVV+VA  + G A NVIP+S TI GTFRAFS KSF
Sbjct: 241 PILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSF 300

Query: 310 NALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVSMEIVGKEKT 369
             L+ RIE+VI  QA V+ C A +DF+ +E P  PPT+ND+ +++  ++VS +++G E  
Sbjct: 301 MQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENY 360

Query: 370 KVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVLPIGAAIHAA 429
                LMGSEDF+F+   +PG F F+G  N+    +  PHSP ++++E +LP GA++HA+
Sbjct: 361 VEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHAS 420

Query: 430 VAYSYLLNSSSTS 443
           +A  YLL   +++
Sbjct: 421 MATRYLLELKAST 430

BLAST of CSPI05G23020 vs. ExPASy Swiss-Prot
Match: Q5N8F2 (IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL2 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.4e-125
Identity = 230/392 (58.67%), Postives = 278/392 (70.92%), Query Frame = 0

Query: 47  VIDLANHPTTVNWMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVA 106
           V+D A  P    WM  +R  IHE PELAFEE ETSRL+R ELD + V+YR PVAGTGVVA
Sbjct: 50  VLDRARRPEFAAWMAGVRWAIHERPELAFEEIETSRLVRAELDAMGVAYRHPVAGTGVVA 109

Query: 107 FVGSGSPPFVALRADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLR 166
            VG+G PPFVALRADMDALP++E V+WEHKSKV  KMHAC HDAH  MLLGA +IL + R
Sbjct: 110 TVGTGRPPFVALRADMDALPMQEEVQWEHKSKVAMKMHACGHDAHTTMLLGAARILQERR 169

Query: 167 HKLQGTVVLVFQPAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAG 226
           H+LQGTVVL+FQP EE G GA+ M+  GA+D VEAIFG HV  E P GVV SRPG  LAG
Sbjct: 170 HELQGTVVLLFQPGEEVGTGARRMVEAGAVDNVEAIFGFHVSVELPTGVVGSRPGPLLAG 229

Query: 227 CGSFKAKIKGKGGHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGT 286
           CG F+A I GKGGHAA P  S+DPILAAS  +++LQ +VSRE DPL++QVV+V    AG 
Sbjct: 230 CGFFEAVITGKGGHAAHPHASVDPILAASTVVLALQGLVSREADPLEAQVVTVTRFLAGD 289

Query: 287 ALNVIPESATIAGTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPT 346
           ALNVIPES TI GTFR FS + F  L+ RIEEVI  Q+ V+RC A +DF     P +PPT
Sbjct: 290 ALNVIPESITIGGTFRVFSNEGFLRLKRRIEEVIVAQSAVYRCAAAVDFHAGGRPLLPPT 349

Query: 347 INDEKIYEHVRHVSMEIVGKEKTKVSPR--LMGSEDFAFFADKVPGS-FLFLGTYNERIG 406
           IN   ++ H + V+ E +G     +      MGSEDFA F++ VP S F F+G  NE  G
Sbjct: 350 INSAALHAHFQAVAAETLGASAAVLGAMEPCMGSEDFAVFSEAVPASHFYFVGVRNEAEG 409

Query: 407 AIHPPHSPRYKIDENVLPIGAAIHAAVAYSYL 436
            +H  HSP +++D+  LP GAA+HA++A  YL
Sbjct: 410 LVHLAHSPHFRVDDAALPYGAALHASLAMRYL 441

BLAST of CSPI05G23020 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 443.0 bits (1138), Expect = 4.1e-123
Identity = 232/439 (52.85%), Postives = 304/439 (69.25%), Query Frame = 0

Query: 2   EFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWMK 61
           + L + F + +FL    S++ ESP +            TS +  ++++ A  P   +WM 
Sbjct: 5   KLLSLTFQLLLFL---LSVSSESPWI---------AEDTSQIQTKLLEFAKSPEVFDWMV 64

Query: 62  TIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRAD 121
            IRRKIHENPEL +EE ETS+LIR EL+ + + YR+PVA TGV+ ++G+G PPFVALRAD
Sbjct: 65  KIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRAD 124

Query: 122 MDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPAE 181
           MDALPI+E VEWEHKSK+ GKMHAC HD HV MLLGA KIL++ RH LQGTVVL+FQPAE
Sbjct: 125 MDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAE 184

Query: 182 EKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 241
           E   GAK M  EGAL  VEAIFG+H+    P G  ASR G FLAG G F+A I GKGGHA
Sbjct: 185 EGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHA 244

Query: 242 AIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGTF 301
           AIPQ +IDP++AAS+ ++SLQ +VSRE DPLDS+VV+V+ V  G A NVIP+S TI GT 
Sbjct: 245 AIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTL 304

Query: 302 RAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVSM 361
           RAF+   F  L+ R++EVI  QA VHRC A ++        +PPT+N++ +Y+  + V  
Sbjct: 305 RAFT--GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVR 364

Query: 362 EIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVLP 421
           +++G+E    +  +MGSEDF++FA+ +PG F  LG  +E  G     HSP Y+I+E+VLP
Sbjct: 365 DLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLP 424

Query: 422 IGAAIHAAVAYSYLLNSSS 441
            GAAIHA++A  YL   +S
Sbjct: 425 YGAAIHASMAVQYLKEKAS 428

BLAST of CSPI05G23020 vs. ExPASy TrEMBL
Match: A0A0A0KQC3 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.4e-251
Identity = 442/448 (98.66%), Postives = 445/448 (99.33%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQ+IDLANHPT VNWM
Sbjct: 35  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 94

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 95  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 154

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 155 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 214

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 215 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 274

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 275 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 334

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPP +NDEKIYEHVR VS
Sbjct: 335 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 394

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL
Sbjct: 395 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 454

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           P+GAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 455 PLGAAIHAAVAYSYLLNSSSTSHSFFDH 482

BLAST of CSPI05G23020 vs. ExPASy TrEMBL
Match: A0A5A7ST02 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003470 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.6e-245
Identity = 435/448 (97.10%), Postives = 441/448 (98.44%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSP LMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of CSPI05G23020 vs. ExPASy TrEMBL
Match: A0A5D3CVM4 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00690 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.2e-244
Identity = 434/448 (96.88%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSP LMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of CSPI05G23020 vs. ExPASy TrEMBL
Match: A0A1S3BE49 (LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103489042 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.8e-242
Identity = 430/448 (95.98%), Postives = 436/448 (97.32%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 1   MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 60

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 360

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEK K     MGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 361 MEIVGKEKQKYLQAXMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 448

BLAST of CSPI05G23020 vs. ExPASy TrEMBL
Match: A0A6J1FSW3 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111446993 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.7e-209
Identity = 370/442 (83.71%), Postives = 400/442 (90.50%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           ME LY L  ISIF        LE+P +HGTT+ S+ T+  SSL HQ+++LAN PTT NWM
Sbjct: 1   MELLYFLIIISIF-------KLEAP-VHGTTASSVQTSQNSSLRHQILELANDPTTSNWM 60

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEFETSRLIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRA
Sbjct: 61  KTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRA 120

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 121 DMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPA 180

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGVE I GLHV+H YPVGVVASRPGEFLAGCGSFKAKIKG+GGH
Sbjct: 181 EERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGH 240

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIP  SIDP+LAAS AI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIP+S TIAGT
Sbjct: 241 AAIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGT 300

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAF KKSFNALRDRIEE+INGQA VHRCT EIDFLGKEHPTIPPTIN+EK+Y+H R VS
Sbjct: 301 FRAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVS 360

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           +EIVGKE TKVSP  MGSEDFAFF+D+VPGSF+FLGTYNERIGAI+PPHSP +KIDENVL
Sbjct: 361 IEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVL 420

Query: 421 PIGAAIHAAVAYSYLLNSSSTS 443
           PIGAAIHAA AYSYLLNSSSTS
Sbjct: 421 PIGAAIHAAFAYSYLLNSSSTS 434

BLAST of CSPI05G23020 vs. NCBI nr
Match: XP_004135471.3 (IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 hypothetical protein Csa_009018 [Cucumis sativus])

HSP 1 Score: 876.3 bits (2263), Expect = 1.1e-250
Identity = 442/448 (98.66%), Postives = 445/448 (99.33%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQ+IDLANHPT VNWM
Sbjct: 38  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 97

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 98  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 157

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 158 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 217

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 218 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 277

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 278 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 337

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPP +NDEKIYEHVR VS
Sbjct: 338 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 397

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL
Sbjct: 398 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 457

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           P+GAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 458 PLGAAIHAAVAYSYLLNSSSTSHSFFDH 485

BLAST of CSPI05G23020 vs. NCBI nr
Match: XP_031741348.1 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 871.3 bits (2250), Expect = 3.6e-249
Identity = 442/450 (98.22%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQ+IDLANHPT VNWM
Sbjct: 38  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 97

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 98  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 157

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 158 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 217

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 218 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 277

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDS--QVVSVAMVQAGTALNVIPESATIA 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDS  QVVSVAMVQAGTALNVIPESATIA
Sbjct: 278 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQLQVVSVAMVQAGTALNVIPESATIA 337

Query: 301 GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRH 360
           GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPP +NDEKIYEHVR 
Sbjct: 338 GTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRR 397

Query: 361 VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDEN 420
           VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDEN
Sbjct: 398 VSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDEN 457

Query: 421 VLPIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           VLP+GAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 458 VLPLGAAIHAAVAYSYLLNSSSTSHSFFDH 487

BLAST of CSPI05G23020 vs. NCBI nr
Match: KAA0034344.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 857.4 bits (2214), Expect = 5.4e-245
Identity = 435/448 (97.10%), Postives = 441/448 (98.44%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSP LMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of CSPI05G23020 vs. NCBI nr
Match: TYK15575.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 854.4 bits (2206), Expect = 4.5e-244
Identity = 434/448 (96.88%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEKTKVSP LMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of CSPI05G23020 vs. NCBI nr
Match: XP_008446258.2 (PREDICTED: LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo])

HSP 1 Score: 845.9 bits (2184), Expect = 1.6e-241
Identity = 430/448 (95.98%), Postives = 436/448 (97.32%), Query Frame = 0

Query: 1   MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWM 60
           MEFLYILFFISIFLPL+FSLNLESPDL+ TTSISL TNHTSSL HQ++DLANHPTTVNWM
Sbjct: 1   MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 60

Query: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120
           KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA
Sbjct: 61  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 120

Query: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180
           DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA
Sbjct: 121 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 180

Query: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EEKGGGAKDMINEGALDGVEAIFGLHVVH+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 181 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240

Query: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300
           AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT
Sbjct: 241 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 300

Query: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVS 360
           FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE+PTIPP INDEKIYEHVR VS
Sbjct: 301 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 360

Query: 361 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 420
           MEIVGKEK K     MGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSP YKIDENVL
Sbjct: 361 MEIVGKEKQKYLQAXMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 420

Query: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 449
           PIGAAIHAAVAYSYLLNSSSTSHSFFDH
Sbjct: 421 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 448

BLAST of CSPI05G23020 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 458.8 bits (1179), Expect = 5.2e-129
Identity = 236/433 (54.50%), Postives = 308/433 (71.13%), Query Frame = 0

Query: 10  ISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWMKTIRRKIHE 69
           +S F  ++F L L    L   T IS  +N  S +  + + LA      +WM  IRR+IHE
Sbjct: 1   MSFFKWVSFVLILH---LLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHE 60

Query: 70  NPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEE 129
           NPEL +EE ETS+L+R EL+ + VSY++PVA TGVV +VG+G  PFVALRADMDAL ++E
Sbjct: 61  NPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQE 120

Query: 130 LVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPAEEKGGGAKD 189
           +VEWEHKSKV GKMHAC HDAH  MLLGA K+L +   +LQGTVVLVFQPAEE GGGAK 
Sbjct: 121 MVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKK 180

Query: 190 MINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQDSID 249
           ++  G L+ V AIFGLHV ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQ +ID
Sbjct: 181 IVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTID 240

Query: 250 PILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGTFRAFSKKSF 309
           PILAAS  I+SLQ +VSRE DPLDSQVV+VA  + G A NVIP+S TI GTFRAFS KSF
Sbjct: 241 PILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSF 300

Query: 310 NALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVSMEIVGKEKT 369
             L+ RIE+VI  QA V+ C A +DF+ +E P  PPT+ND+ +++  ++VS +++G E  
Sbjct: 301 MQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENY 360

Query: 370 KVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVLPIGAAIHAA 429
                LMGSEDF+F+   +PG F F+G  N+    +  PHSP ++++E +LP GA++HA+
Sbjct: 361 VEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHAS 420

Query: 430 VAYSYLLNSSSTS 443
           +A  YLL   +++
Sbjct: 421 MATRYLLELKAST 430

BLAST of CSPI05G23020 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 443.0 bits (1138), Expect = 2.9e-124
Identity = 232/439 (52.85%), Postives = 304/439 (69.25%), Query Frame = 0

Query: 2   EFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQVIDLANHPTTVNWMK 61
           + L + F + +FL    S++ ESP +            TS +  ++++ A  P   +WM 
Sbjct: 5   KLLSLTFQLLLFL---LSVSSESPWI---------AEDTSQIQTKLLEFAKSPEVFDWMV 64

Query: 62  TIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRAD 121
            IRRKIHENPEL +EE ETS+LIR EL+ + + YR+PVA TGV+ ++G+G PPFVALRAD
Sbjct: 65  KIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRAD 124

Query: 122 MDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPAE 181
           MDALPI+E VEWEHKSK+ GKMHAC HD HV MLLGA KIL++ RH LQGTVVL+FQPAE
Sbjct: 125 MDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAE 184

Query: 182 EKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 241
           E   GAK M  EGAL  VEAIFG+H+    P G  ASR G FLAG G F+A I GKGGHA
Sbjct: 185 EGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHA 244

Query: 242 AIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGTF 301
           AIPQ +IDP++AAS+ ++SLQ +VSRE DPLDS+VV+V+ V  G A NVIP+S TI GT 
Sbjct: 245 AIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTL 304

Query: 302 RAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRHVSM 361
           RAF+   F  L+ R++EVI  QA VHRC A ++        +PPT+N++ +Y+  + V  
Sbjct: 305 RAFT--GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVR 364

Query: 362 EIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVLP 421
           +++G+E    +  +MGSEDF++FA+ +PG F  LG  +E  G     HSP Y+I+E+VLP
Sbjct: 365 DLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLP 424

Query: 422 IGAAIHAAVAYSYLLNSSS 441
            GAAIHA++A  YL   +S
Sbjct: 425 YGAAIHASMAVQYLKEKAS 428

BLAST of CSPI05G23020 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 440.3 bits (1131), Expect = 1.9e-123
Identity = 216/401 (53.87%), Postives = 286/401 (71.32%), Query Frame = 0

Query: 40  TSSLTHQVIDLANHPTTVNWMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPV 99
           T + + +++ L   P  V W+K +RR IHENPELAFEE+ETSRLIR ELD + + YR+P+
Sbjct: 69  TKACSDEILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPL 128

Query: 100 AGTGVVAFVGSGSPPFVALRADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGAT 159
           A TG+ A++GSG PPFVA+RADMDALPI+E VEWEH SKV GKMHAC HDAHV MLLGA 
Sbjct: 129 AKTGIRAWIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAA 188

Query: 160 KILNQLRHKLQGTVVLVFQPAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASR 219
            IL    H L+GTVVL+FQPAEE G GAK+MI +GALD VEAIF +HV H +P GV+ SR
Sbjct: 189 HILKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSR 248

Query: 220 PGEFLAGCGSFKAKIKGKGGHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSV 279
            G  LAGCG F+A I  +    A      + +LAAS+A+ISLQ IVSRE  PLDSQVVSV
Sbjct: 249 SGPLLAGCGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSV 308

Query: 280 AMVQAGTALNVIPESATIAGTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKE 339
                G +L+V P++  + GTFRAFS  SF  L+ RI+EV+  Q  V  C A ++F  K+
Sbjct: 309 TSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQ 368

Query: 340 HPTIPPTINDEKIYEHVRHVSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYN 399
           +   PPT N++  Y H++ V+++++G     ++P++MG+EDFAF+++ +P +F F+G  N
Sbjct: 369 NAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRN 428

Query: 400 ERIGAIHPPHSPRYKIDENVLPIGAAIHAAVAYSYLLNSSS 441
           E +G++H  HSP + IDE+ LP+GAA+HAAVA  YL +  S
Sbjct: 429 EELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKHS 464

BLAST of CSPI05G23020 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 435.3 bits (1118), Expect = 6.1e-122
Identity = 220/409 (53.79%), Postives = 291/409 (71.15%), Query Frame = 0

Query: 33  ISLPTNHTSSLTHQVIDLANHPTTVNWMKTIRRKIHENPELAFEEFETSRLIRQELDNLR 92
           IS  +N  S +    + LA      +WM  IRR+IHENPEL +EE ETS+L++ ELD + 
Sbjct: 21  ISCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMG 80

Query: 93  VSYRWPVAGTGVVAFVGSGSPPFVALRADMDALPIEELVEWEHKSKVEGKMHACSHDAHV 152
           VSY+ PVA TGV+ +VG+G  PFVALRADMDALPI+E+VEWEHKSK+ GKMHAC HDAH 
Sbjct: 81  VSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHT 140

Query: 153 AMLLGATKILNQLRHKLQGTVVLVFQPAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYP 212
            MLLGA K+L + + +LQGTV+LVFQPAEE G GAK ++  G L+ V AIFGLHV +   
Sbjct: 141 TMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLG 200

Query: 213 VGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQDSIDPILAASAAIISLQSIVSREIDPL 272
           +G ++SR G  +AG G FKA I GKGGHAA+PQ +IDP+LAAS  I+SLQ +VSRE DPL
Sbjct: 201 LGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPL 260

Query: 273 DSQVVSVAMVQAGTALNVIPESATIAGTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAE 332
           DSQVV+VA  +   A NVIP+S TI GTFRA   KSF  L+ RI +VI  QA V+ C A 
Sbjct: 261 DSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNAT 320

Query: 333 IDFLGKEHPTIPPTINDEKIYEHVRHVSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSF 392
           +DFL  E P  PPT+N++ ++   ++VS++++G E    +  +M SEDFAF+   +PG F
Sbjct: 321 VDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHF 380

Query: 393 LFLGTYNERIGAIHPPHSPRYKIDENVLPIGAAIHAAVAYSYLLNSSST 442
            F+G  N+    +  PHSP ++++E +LP GA++ A++A  YLL+SSS+
Sbjct: 381 SFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSSS 429

BLAST of CSPI05G23020 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 432.2 bits (1110), Expect = 5.2e-121
Identity = 221/393 (56.23%), Postives = 280/393 (71.25%), Query Frame = 0

Query: 48  IDLANHPTTVNWMKTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAF 107
           ++LA  P   + M  IRRKIHENPEL +EEFETS+ IR ELD + V YR+PVA TG++ +
Sbjct: 38  LELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFPVAITGIIGY 97

Query: 108 VGSGSPPFVALRADMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRH 167
           +G+G PPFVALRADMDALPI+E VEWEHKSK  GKMHAC HD HVAMLLGA KIL Q R 
Sbjct: 98  IGTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQ 157

Query: 168 KLQGTVVLVFQPAEEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGC 227
            LQGTVVL+FQPAEE   GAK M  EGAL  VEAIFG+H+    P G  AS  G F+AG 
Sbjct: 158 HLQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGA 217

Query: 228 GSFKAKIKGKGGHAAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTA 287
           G+F+A I GKGGHAAIPQ +IDP++AAS+ ++SLQ +VSRE DP DS+VV+V  V  G A
Sbjct: 218 GAFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNA 277

Query: 288 LNVIPESATIAGTFRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPTI 347
            NVIP+S TI GT RAF+   F  L++RI+E+I  QA VHRC A ++     +  +PPT+
Sbjct: 278 FNVIPDSITIGGTLRAFT--GFTQLQERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTV 337

Query: 348 NDEKIYEHVRHVSMEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHP 407
           N+  +Y+  + V  +++G+E    +   MGSEDF++FA+ +PG F  LG  +E  G    
Sbjct: 338 NNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YAS 397

Query: 408 PHSPRYKIDENVLPIGAAIHAAVAYSYLLNSSS 441
            HSP Y+I+E+VLP GAAIHA +A  YL + +S
Sbjct: 398 SHSPHYRINEDVLPYGAAIHATMAVQYLKDKAS 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S9S41.0e-12961.74IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q84XG93.0e-12961.48IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
O043737.3e-12854.50IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
Q5N8F23.4e-12558.67IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P549704.1e-12352.85IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KQC35.4e-25198.66M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 P... [more]
A0A5A7ST022.6e-24597.10IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CVM42.2e-24496.88IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BE497.8e-24295.98LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=365... [more]
A0A6J1FSW36.7e-20983.71IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
Match NameE-valueIdentityDescription
XP_004135471.31.1e-25098.66IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 ... [more]
XP_031741348.13.6e-24998.22IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucumis sativus][more]
KAA0034344.15.4e-24597.10IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa][more]
TYK15575.14.5e-24496.88IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa][more]
XP_008446258.21.6e-24195.98PREDICTED: LOW QUALITY PROTEIN: IAA-amino acid hydrolase ILR1-like 4 [Cucumis me... [more]
Match NameE-valueIdentityDescription
AT1G51760.15.2e-12954.50peptidase M20/M25/M40 family protein [more]
AT5G56660.12.9e-12452.85IAA-leucine resistant (ILR)-like 2 [more]
AT1G44350.11.9e-12353.87IAA-leucine resistant (ILR)-like gene 6 [more]
AT1G51780.16.1e-12253.79IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56650.15.2e-12156.23IAA-leucine resistant (ILR)-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 226..324
e-value: 9.9E-11
score: 41.5
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 61..423
e-value: 9.4E-119
score: 394.7
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 48..439
e-value: 9.1E-128
score: 424.4
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 5..443
NoneNo IPR availableGENE3D3.30.70.360coord: 225..343
e-value: 2.5E-140
score: 469.4
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 62..420
e-value: 2.5E-140
score: 469.4
NoneNo IPR availablePANTHERPTHR11014:SF147IAA-AMINO ACID HYDROLASE ILR1-LIKE 2coord: 5..443
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 56..437
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 117..431
e-value: 1.8E-33
score: 116.4
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 60..435
e-value: 0.0
score: 652.847
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 226..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G23020.1CSPI05G23020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity