CSPI05G21810 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G21810
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionVery-long-chain 3-oxoacyl-CoA reductase-like protein
LocationChr5: 22425242 .. 22426890 (+)
RNA-Seq ExpressionCSPI05G21810
SyntenyCSPI05G21810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTATGTAATCAAATTCTCAACTCTCTTCCTTAATATATTTTCTTGTTCTTGTTCTTGTTGTTCTTCTTTTAGGGAATAAAGAAAATGAAGAAGGTTTGAGGGAAGAAGTCCATCTCTTTCTCTATCTTCCTGAGGAAATAAGTGTTCACTGAGATGGGCCTCCCTCACTTACCCCATGTCTCACCAACTACCATGTCTTCTGCCAAAGTTCCTAATTAATTTTTGAGAAATTATTGACGTCTTCTCTGCTTTTTGAAATAAGAAAAAGTACAGGTACGGTTGAAAAGGTTTTGGCATGTGCTGTTGTCAGAAGTTTATTCAAAATCACTCCAAAATATGTTGTTAGTCCTTCTAAATCAAAGTTGATGATATGAAAATTGGCTTTAAAAGTGTAATTAAAGTCATGTTTCCGAGTCATTTTTAACATGACATGATTGATTTCAACAATTTCAAAAACACTCTCAAAACGTGGGAAATCCAACTCATTTTTCCATCCGTACACGGGTCCCCAACTTCCACCTCCAAGTTAAAAACCTCTACTTTTTCTGATTATGGGATAATCGAAAAGCTGAAAAGCAACCCTCCATTTGATCCTTGTTGAATGTTGATGATATGATATAATCATGTTGATCTTTCTAATTCTTTCTTTCTTTTCTCAGGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA

mRNA sequence

ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA

Coding sequence (CDS)

ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA

Protein sequence

MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGLERRRKESVTMKRIEGCKQE*
Homology
BLAST of CSPI05G21810 vs. ExPASy Swiss-Prot
Match: Q9FYL6 (Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis thaliana OX=3702 GN=KCR2 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.4e-97
Identity = 177/304 (58.22%), Postives = 226/304 (74.34%), Query Frame = 0

Query: 11  TSPIWLLILSFLGF-FTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSF 70
           + P +L  + F+GF F +      + +W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 71  AYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVI 130
           A++LA+ GLNL+LVSR+  KL++VS D Q EFP  K+KII  DF+ +   GG   IEE I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 131 EDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAI 190
           + L+VGILINNVGITYP A FFHEVD+  W  + +VN++ TTWVT+ ++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 191 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASR 250
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 251 VASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIG 310
           VA++ + SLF+PS + Y KAA+ QIG   RC+P+WAHSLQW    L+P+ ++D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 311 LERR 314
           L RR
Sbjct: 309 LRRR 309

BLAST of CSPI05G21810 vs. ExPASy Swiss-Prot
Match: Q8L9C4 (Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=KCR1 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.6e-87
Identity = 162/305 (53.11%), Postives = 220/305 (72.13%), Query Frame = 0

Query: 11  TSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFA 70
           + P WLLIL  LG  +I     T+ R  Y  FLRPSK+LR YGSW I+TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 71  YQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVIE 130
           +QLA+ GLNL+LV+R+  KLK VS  I+S++  T++  + +DF+  DI  G+  I+E IE
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 131 DLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIV 190
            LDVGILINN G++YP A +FHEVDE++  N+ K+NV+GTT VT+ VLP M+K+ +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 191 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRV 250
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 251 ASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGL 310
             + RAS  + S + Y KAA+R +GYE +CTPYW H+L     S LPE++ +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 311 ERRRK 316
           + R+K
Sbjct: 309 QIRKK 308

BLAST of CSPI05G21810 vs. ExPASy Swiss-Prot
Match: Q3SXM5 (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens OX=9606 GN=HSDL1 PE=1 SV=3)

HSP 1 Score: 194.1 bits (492), Expect = 2.5e-48
Identity = 108/274 (39.42%), Postives = 159/274 (58.03%), Query Frame = 0

Query: 49  LRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKI 108
           ++ YG W +V+GATDGIGK++A +LA  GLN++L+SR+  KL+ V+KDI   +       
Sbjct: 63  IKQYGRWAVVSGATDGIGKAYAEELASRGLNIILISRNEEKLQVVAKDIADTYK------ 122

Query: 109 IELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKV 168
           +E D    D S G      I E ++D DVGIL+NNVG+ YP   +F ++ E    ++  V
Sbjct: 123 VETDIIVADFSSGREIYLPIREALKDKDVGILVNNVGVFYPYPQYFTQLSEDKLWDIINV 182

Query: 169 NVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHV 228
           N+   + +  VVLP M+++ +GAIV I SG+       P  A ++A+KAY+D  SR+L  
Sbjct: 183 NIAAASLMVHVVLPGMVERKKGAIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQY 242

Query: 229 EYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWA 288
           EY   GI VQ  +P YVAT M +    + R S  +PS   Y   A+  +G   R T YW+
Sbjct: 243 EYASKGIFVQSLIPFYVATSMTAPSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWS 302

Query: 289 HSLQWCFASLLPEAMLDAWRLSIGLERRRKESVT 320
           HS+Q+ FA  +PE  L  W  +I     RKE+++
Sbjct: 303 HSIQFLFAQYMPE-WLWVWGANILNRSLRKEALS 327

BLAST of CSPI05G21810 vs. ExPASy Swiss-Prot
Match: A5PJF6 (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus OX=9913 GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-48
Identity = 112/291 (38.49%), Postives = 167/291 (57.39%), Query Frame = 0

Query: 34  VARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAV 93
           + R V RA L     ++ YG W +V+GATDGIG+++A +LA  GLN+VL+SRS  KL+ V
Sbjct: 53  IPRLVSRADL-----IKQYGRWAVVSGATDGIGRAYAEELASRGLNIVLISRSQEKLQMV 112

Query: 94  SKDIQSEFPDTKVKIIELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASF 153
           +KDI   +       +E D    D S G      I E ++D D+GIL+NNVG+ YP   +
Sbjct: 113 AKDIADTYK------VETDIIVADFSSGREIYDMIREALQDRDIGILVNNVGVFYPYPQY 172

Query: 154 FHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYA 213
           F +V E    ++  VN+   + +  +VLP M+++ +GAIV I SG+       P  A ++
Sbjct: 173 FTQVSEDTLWDIVNVNIAAASLMVHIVLPGMVERKKGAIVTISSGSC--CKPTPQLAAFS 232

Query: 214 ATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAA 273
           A+KAY+D  SR+L  EY   GI VQ  +P YVAT +A+  + + +    +PS   Y   A
Sbjct: 233 ASKAYLDHFSRALQYEYASKGIFVQSLIPFYVATNVATPGSFLHKCPWLVPSPKVYAHHA 292

Query: 274 IRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGLERRRKESVTMK 322
           +  +G   R T YW+HS+Q+ FA  +PE  L  W  +I     RKE+++ K
Sbjct: 293 VSTLGISKRTTGYWSHSIQFLFAQYMPE-WLWVWGANILNRSLRKEALSCK 329

BLAST of CSPI05G21810 vs. ExPASy Swiss-Prot
Match: Q5R7K0 (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Pongo abelii OX=9601 GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-47
Identity = 105/274 (38.32%), Postives = 159/274 (58.03%), Query Frame = 0

Query: 49  LRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKI 108
           ++ YG W +V+GATDGIG+++A +LA  GLN++L+SR+  KL+ V+KDI   +       
Sbjct: 63  IKQYGRWAVVSGATDGIGRAYAEELASRGLNIILISRNEEKLQVVAKDIADTYK------ 122

Query: 109 IELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKV 168
           +E      D S G      I E ++D D+GIL+NNVG+ YP   +F ++ E    ++  V
Sbjct: 123 VETGIIVADFSSGREIYLPIREALKDKDIGILVNNVGVFYPYPQYFTQLSEDKLWDIINV 182

Query: 169 NVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHV 228
           N+   + +  VVLP M+++ +GAIV I SG+       P  A ++A+KAY+D  SR+L  
Sbjct: 183 NIAAASLMVHVVLPGMVERKKGAIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQY 242

Query: 229 EYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWA 288
           EY   GI VQ  +P YVAT M +  + + R S  +PS   Y   A+  +G   R T YW+
Sbjct: 243 EYASKGIFVQSLIPFYVATSMTAPSSFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWS 302

Query: 289 HSLQWCFASLLPEAMLDAWRLSIGLERRRKESVT 320
           HS+Q+ FA  +PE  L  W  +I     RKE+++
Sbjct: 303 HSIQFLFAQYMPE-WLWVWGANILNRSLRKEALS 327

BLAST of CSPI05G21810 vs. ExPASy TrEMBL
Match: A0A0A0KS33 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G591790 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 327/329 (99.39%), Postives = 328/329 (99.70%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVS+ASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM
Sbjct: 244 YVATEMASRVASVSQASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGLERRRKESVTMKRIEGCKQE
Sbjct: 304 LDAWRLSIGLERRRKESVTMKRIEGCKQE 332

BLAST of CSPI05G21810 vs. ExPASy TrEMBL
Match: A0A5D3CW53 (Very-long-chain 3-oxoacyl-CoA reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001930 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.8e-171
Identity = 308/329 (93.62%), Postives = 318/329 (96.66%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLST+PIWLLILS LGFFTIL HSATVARWV+RAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNL+LVSRSS KLKAVS+DIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMN+FKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLF+PSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFA LLPEA+
Sbjct: 244 YVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLPEAV 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL+RR   SV M+  EGCKQE
Sbjct: 304 LDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

BLAST of CSPI05G21810 vs. ExPASy TrEMBL
Match: A0A1S3BE86 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucumis melo OX=3656 GN=LOC103488924 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.8e-171
Identity = 308/329 (93.62%), Postives = 318/329 (96.66%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLST+PIWLLILS LGFFTIL HSATVARWV+RAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNL+LVSRSS KLKAVS+DIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMN+FKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLF+PSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFA LLPEA+
Sbjct: 244 YVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLPEAV 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL+RR   SV M+  EGCKQE
Sbjct: 304 LDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

BLAST of CSPI05G21810 vs. ExPASy TrEMBL
Match: A0A6J1KC16 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita maxima OX=3661 GN=LOC111492945 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.4e-160
Identity = 286/329 (86.93%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           M+ETFFLLLST PIWLL+LS LGFFTIL HS T  +WV+R FLRPSKDLRYYGSWG+VTG
Sbjct: 4   MRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTAVQWVFRVFLRPSKDLRYYGSWGVVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSF+YQLARAGLNL+LVSRSS KLKAVSKDI+S+FP+TKVKIIELDFTEDDIS 
Sbjct: 64  ATDGIGKSFSYQLARAGLNLILVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDDISA 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           G+AE ++VI+DLDVG+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTK VLP+
Sbjct: 124 GMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPR 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQVPL
Sbjct: 184 MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLFIPSADDYV AAI +IGYEPRCTPYWAHSLQWCFA LLPEA 
Sbjct: 244 YVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAA 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LD WRLSIGL RRRK    MK IEG KQE
Sbjct: 304 LDMWRLSIGLHRRRKGLEAMKGIEGYKQE 332

BLAST of CSPI05G21810 vs. ExPASy TrEMBL
Match: A0A6J1GZN0 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita moschata OX=3662 GN=LOC111458680 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 281/315 (89.21%), Postives = 295/315 (93.65%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           M+ETFFLLLST PIWLL+LS LGFFTIL HS TV +WV+R FLRPSKDLRYYGSWG+VTG
Sbjct: 4   MRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGVVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNLVLVSRSS KLKAVSKDI+S+FP+TKVKIIELDFTEDDIS 
Sbjct: 64  ATDGIGKSFAYQLARAGLNLVLVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDDISA 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           G+AE ++VI+DLDVG+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTK VLPK
Sbjct: 124 GMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQVPL
Sbjct: 184 MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVS ASLFIPSADDYV AAI +IGYEPRCTPYWAHSLQWCFA LLPEA 
Sbjct: 244 YVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAA 303

Query: 301 LDAWRLSIGLERRRK 316
           LDAWRLSIGL RR K
Sbjct: 304 LDAWRLSIGLHRRSK 318

BLAST of CSPI05G21810 vs. NCBI nr
Match: XP_004135293.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucumis sativus] >KGN51714.1 hypothetical protein Csa_008307 [Cucumis sativus])

HSP 1 Score: 648.3 bits (1671), Expect = 3.6e-182
Identity = 327/329 (99.39%), Postives = 328/329 (99.70%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVS+ASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM
Sbjct: 244 YVATEMASRVASVSQASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGLERRRKESVTMKRIEGCKQE
Sbjct: 304 LDAWRLSIGLERRRKESVTMKRIEGCKQE 332

BLAST of CSPI05G21810 vs. NCBI nr
Match: XP_008446096.1 (PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucumis melo] >KAA0034225.1 very-long-chain 3-oxoacyl-CoA reductase-like protein [Cucumis melo var. makuwa] >TYK15695.1 very-long-chain 3-oxoacyl-CoA reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 611.7 bits (1576), Expect = 3.8e-171
Identity = 308/329 (93.62%), Postives = 318/329 (96.66%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLST+PIWLLILS LGFFTIL HSATVARWV+RAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNL+LVSRSS KLKAVS+DIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMN+FKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLF+PSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFA LLPEA+
Sbjct: 244 YVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLPEAV 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL+RR   SV M+  EGCKQE
Sbjct: 304 LDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

BLAST of CSPI05G21810 vs. NCBI nr
Match: XP_038892142.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Benincasa hispida])

HSP 1 Score: 592.0 bits (1525), Expect = 3.1e-165
Identity = 296/329 (89.97%), Postives = 314/329 (95.44%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFL+LST P+WLLILS LGFFTIL HS+TV +WV+RAFLRPSKDLRYYGSWG++TG
Sbjct: 4   MKETFFLVLSTQPVWLLILSLLGFFTILRHSSTVVQWVFRAFLRPSKDLRYYGSWGVITG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLN++LVSRSS KLKAVSKDIQSEFPDTKVKIIELDFT+DDIS 
Sbjct: 64  ATDGIGKSFAYQLARAGLNVILVSRSSTKLKAVSKDIQSEFPDTKVKIIELDFTDDDISA 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEI+EVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIKEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKN+GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNKGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLFIPSADDYVKA+IR+IGYEPRCTPYWAHSLQWCFA LLPEA 
Sbjct: 244 YVATEMASRVASVSRASLFIPSADDYVKASIRRIGYEPRCTPYWAHSLQWCFACLLPEAT 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL+RR  ++  MK IE CKQ+
Sbjct: 304 LDAWRLSIGLQRRGSDA--MKGIEDCKQD 330

BLAST of CSPI05G21810 vs. NCBI nr
Match: XP_023517363.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.9 bits (1478), Expect = 8.7e-160
Identity = 288/329 (87.54%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           M+ETFFLLLST PIWLL+LS LGFFTIL HS TV +WV+R FLRPSKDLRYYGSWG+VTG
Sbjct: 4   MRETFFLLLSTQPIWLLLLSLLGFFTILSHSTTVVQWVFRVFLRPSKDLRYYGSWGVVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNL+LVSRSS KLK VSKDI+S+FP+TKVKIIELDFTEDDIS 
Sbjct: 64  ATDGIGKSFAYQLARAGLNLILVSRSSTKLKEVSKDIRSQFPNTKVKIIELDFTEDDISA 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           G+AE ++VI+DLDVG+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTK VLPK
Sbjct: 124 GMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQVPL
Sbjct: 184 MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLFIPSADDYV AAI +IGYEPRCTPYWAHSLQWCFA LLPEA 
Sbjct: 244 YVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAA 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL RR K    MK IEG KQE
Sbjct: 304 LDAWRLSIGLHRRSKGLDAMKGIEGYKQE 332

BLAST of CSPI05G21810 vs. NCBI nr
Match: XP_022998255.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita maxima])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 286/329 (86.93%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           M+ETFFLLLST PIWLL+LS LGFFTIL HS T  +WV+R FLRPSKDLRYYGSWG+VTG
Sbjct: 4   MRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTAVQWVFRVFLRPSKDLRYYGSWGVVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSF+YQLARAGLNL+LVSRSS KLKAVSKDI+S+FP+TKVKIIELDFTEDDIS 
Sbjct: 64  ATDGIGKSFSYQLARAGLNLILVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDDISA 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           G+AE ++VI+DLDVG+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTK VLP+
Sbjct: 124 GMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPR 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQVPL
Sbjct: 184 MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLFIPSADDYV AAI +IGYEPRCTPYWAHSLQWCFA LLPEA 
Sbjct: 244 YVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAA 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LD WRLSIGL RRRK    MK IEG KQE
Sbjct: 304 LDMWRLSIGLHRRRKGLEAMKGIEGYKQE 332

BLAST of CSPI05G21810 vs. TAIR 10
Match: AT1G24470.1 (beta-ketoacyl reductase 2 )

HSP 1 Score: 355.5 bits (911), Expect = 4.5e-98
Identity = 177/304 (58.22%), Postives = 226/304 (74.34%), Query Frame = 0

Query: 11  TSPIWLLILSFLGF-FTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSF 70
           + P +L  + F+GF F +      + +W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 71  AYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVI 130
           A++LA+ GLNL+LVSR+  KL++VS D Q EFP  K+KII  DF+ +   GG   IEE I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 131 EDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAI 190
           + L+VGILINNVGITYP A FFHEVD+  W  + +VN++ TTWVT+ ++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 191 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASR 250
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 251 VASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIG 310
           VA++ + SLF+PS + Y KAA+ QIG   RC+P+WAHSLQW    L+P+ ++D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 311 LERR 314
           L RR
Sbjct: 309 LRRR 309

BLAST of CSPI05G21810 vs. TAIR 10
Match: AT1G67730.1 (beta-ketoacyl reductase 1 )

HSP 1 Score: 322.8 bits (826), Expect = 3.3e-88
Identity = 162/305 (53.11%), Postives = 220/305 (72.13%), Query Frame = 0

Query: 11  TSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFA 70
           + P WLLIL  LG  +I     T+ R  Y  FLRPSK+LR YGSW I+TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 71  YQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVIE 130
           +QLA+ GLNL+LV+R+  KLK VS  I+S++  T++  + +DF+  DI  G+  I+E IE
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 131 DLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIV 190
            LDVGILINN G++YP A +FHEVDE++  N+ K+NV+GTT VT+ VLP M+K+ +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 191 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRV 250
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 251 ASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGL 310
             + RAS  + S + Y KAA+R +GYE +CTPYW H+L     S LPE++ +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 311 ERRRK 316
           + R+K
Sbjct: 309 QIRKK 308

BLAST of CSPI05G21810 vs. TAIR 10
Match: AT5G10050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-14
Identity = 64/202 (31.68%), Postives = 107/202 (52.97%), Query Frame = 0

Query: 57  IVTGATD-GIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTE 116
           ++TG +  GIG + A +    G  +V  SRS    ++   D++    D+++ + ELD   
Sbjct: 11  LITGCSQGGIGHALAREFTEKGCRVVATSRS----RSTMTDLEQ---DSRLFVKELDVQS 70

Query: 117 D-DISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVT 176
           D ++S  ++E+ +    +DV  L+NN G+         E       N F  NV G+  +T
Sbjct: 71  DQNVSKVLSEVIDKFGKIDV--LVNNAGVQCVGP--LAETPISAMENTFNTNVFGSMRMT 130

Query: 177 KVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDV 236
           + V+P M+ K +G IVN+GS   V+ P  P   +Y ATKA +  L+ +L +E + +GIDV
Sbjct: 131 QAVVPHMVSKKKGKIVNVGS-ITVMAPG-PWAGVYTATKAAIHALTDTLRLELRPFGIDV 190

Query: 237 QCQVPLYVATEMA-SRVASVSR 256
              VP  + T +A S VA+ ++
Sbjct: 191 INVVPGGIRTNIANSAVATFNK 199

BLAST of CSPI05G21810 vs. TAIR 10
Match: AT3G55290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-12
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 57  IVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTED 116
           +VTGA+ GIG+     LA+AG  ++  +R   +L ++  +I S F  T ++   L+    
Sbjct: 24  LVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINS-FSSTGIQAAALEL--- 83

Query: 117 DISGGIAEIEEVIEDL-----DVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTT 176
           D+S   A I++ + +       +  LINN GI   N     ++ E  W NVFK N+KG  
Sbjct: 84  DVSSDAATIQKAVREAWDIFGKIDALINNAGIR-GNVKSSLDLSEDEWDNVFKTNLKGPW 143

Query: 177 WVTKVVLPKMIKKNR-GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVE 227
            V+K V   M    R G+++NI S  A I    P    YA +K  VD +SR + +E
Sbjct: 144 LVSKHVCMLMRDAKRGGSVINI-SSIAGIRGMLPGGLAYACSKGGVDTMSRMMALE 193

BLAST of CSPI05G21810 vs. TAIR 10
Match: AT3G55290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-12
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 57  IVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTED 116
           +VTGA+ GIG+     LA+AG  ++  +R   +L ++  +I S F  T ++   L+    
Sbjct: 23  LVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINS-FSSTGIQAAALEL--- 82

Query: 117 DISGGIAEIEEVIEDL-----DVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTT 176
           D+S   A I++ + +       +  LINN GI   N     ++ E  W NVFK N+KG  
Sbjct: 83  DVSSDAATIQKAVREAWDIFGKIDALINNAGIR-GNVKSSLDLSEDEWDNVFKTNLKGPW 142

Query: 177 WVTKVVLPKMIKKNR-GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVE 227
            V+K V   M    R G+++NI S  A I    P    YA +K  VD +SR + +E
Sbjct: 143 LVSKHVCMLMRDAKRGGSVINI-SSIAGIRGMLPGGLAYACSKGGVDTMSRMMALE 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYL66.4e-9758.22Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis th... [more]
Q8L9C44.6e-8753.11Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=KCR... [more]
Q3SXM52.5e-4839.42Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens OX=9606 GN=... [more]
A5PJF64.2e-4838.49Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus OX=9913 GN=HS... [more]
Q5R7K02.1e-4738.32Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Pongo abelii OX=9601 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KS331.8e-18299.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G591790 PE=3 SV=1[more]
A0A5D3CW531.8e-17193.62Very-long-chain 3-oxoacyl-CoA reductase-like protein OS=Cucumis melo var. makuwa... [more]
A0A1S3BE861.8e-17193.62very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucumis melo O... [more]
A0A6J1KC169.4e-16086.93very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita maxi... [more]
A0A6J1GZN01.3e-15689.21very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
XP_004135293.13.6e-18299.39very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucumis sativus]... [more]
XP_008446096.13.8e-17193.62PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucum... [more]
XP_038892142.13.1e-16589.97very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Benincasa hispid... [more]
XP_023517363.18.7e-16087.54very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita pepo s... [more]
XP_022998255.11.9e-15986.93very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT1G24470.14.5e-9858.22beta-ketoacyl reductase 2 [more]
AT1G67730.13.3e-8853.11beta-ketoacyl reductase 1 [more]
AT5G10050.11.1e-1431.68NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55290.11.3e-1232.95NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55290.21.3e-1232.95NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 132..143
score: 38.54
coord: 181..197
score: 34.82
coord: 55..72
score: 37.93
coord: 209..228
score: 34.59
coord: 230..247
score: 29.78
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 132..143
score: 42.28
coord: 187..195
score: 54.45
coord: 209..228
score: 33.66
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 57..250
e-value: 1.0E-43
score: 149.1
NoneNo IPR availablePIRSFPIRSF00012611-beta-HSD1coord: 6..318
e-value: 5.8E-97
score: 322.2
NoneNo IPR availableGENE3D3.40.50.720coord: 50..281
e-value: 2.0E-52
score: 180.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 57..255
e-value: 2.7E-18
score: 64.0
NoneNo IPR availablePANTHERPTHR43899RH59310Pcoord: 15..316
NoneNo IPR availablePANTHERPTHR43899:SF26ENOYL-(ACYL CARRIER) REDUCTASEcoord: 15..316
NoneNo IPR availableCDDcd0535617beta-HSD1_like_SDR_ccoord: 53..298
e-value: 1.77378E-100
score: 293.741
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 196..224
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 51..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G21810.1CSPI05G21810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045703 ketoreductase activity
molecular_function GO:0016491 oxidoreductase activity