CSPI05G21370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G21370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPolysacc_synt_4 domain-containing protein
LocationChr5: 22128034 .. 22129784 (+)
RNA-Seq ExpressionCSPI05G21370
SyntenyCSPI05G21370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGATGGCTTTGGTAGAAGCAAGGACCAAGTTGATACACGTGGTGAGAGGTAAGTGGATGAACAATTTAGAACGGATCCCACAAGGAGTAAGAACATCCAATCATCATCTGAGTCAGCGAAACGTTGAATAACGAACCGATTTTACCAAAGAACCGGTCAACGACCATAGGGTAAAGCTCAAAATGGTCCTCATGAGAAAGGGTATTTCAGTCTTTTCAACTACCGTCCAATCTCAACACCGTTTCCCTAAACCTTTAAACGAACCGAACGCATCGCTCAGATTTCTTCATTTTGGAGAGTTTGAATTTTTGTTTTTTTTTTTTGAAATTTTTTGTTTCTCATCAAACTGCAAATGAAGAATCGAATATCAGCGAAGCCATGGATTCTCGGATTGGCCGTCGCCGGCTTAATCGCGGGTTCTTTATTGATTTCCGGTTACATAGGATTTGTGGACAGCAACTTATTCTGCTCAATCTCCCGCACCGGAGCACCTACAATAACCGGGTATTCACCGGCGGAAGTCCAGTTTCAGTCTATAATCCATTACGCAACCTCGAAAACAGTTCCACAACAATCAATCGACGAGATCCATATCTCATACGACGTATTGAAGGTACGATGTCCTTGCAACTTCCTTGTTTTCGGGCTCGGCCACGATTCCTTGATGTGGGCGTCGCTTAACCCACTTGGAACTACGATTTTTCTCGAAGAAGATCCGAAATGGGTTCAGACGGTTCTTAAAGACGCTCCGATGCTTCGAGCTTATCACGTCCAGTATCGGACGCAGCTTCAGGAAGCAGATCGGTTGCTCTCAACCTACAAATCAGAGCCATATTGTTCGCCGTCGAAGGCATTTCTTAAGGGAAATGAAAAGTGTAAGTTAGCGCTTCATAATCTCCCAGAAGAGATTTACGAGAAGGAATGGGACCTGATAATGATCGACGCGCCAAGAGGGTATTTTGCGGAGGCTCCTGGTCGGATGGCGGCGATATTCTCCGCCACCGTGATGGCAAGGAATAGGAAAGGATCCGGCGTTACTGATGTGTTTTTGCACGACGTTGATCGGAAAGTGGAGAGAACATTTGCACAGGAGTTTTTGTGTAAAAATAAGTATTATGTAAACGGAGTTGGAAGGTTATGGCATTTCAAGATTCCTCCGGCGGCCAATATGAGCGAAGCCGATCGATCTTCCGATCGATTTTGTTAGATCAGAAGGGATTTGTTCACCGGAAATTATTTTTGTCTAAAGATAGTTATCGGTCTAGGGACCGGCGGTGAGGGAGTTGAAGGGAAAATTTTCTTTGGATATCAATAAAAAACCGCCGGAGTTTGAGCTCATGGGTCGGACGGACAATTTTGGCCGTCAACTCCGGCTGTGTTTTCGGCCGAAAAAAAAGTAGAAGATTTCAATCTTCTCCTTAATTGACTTTTTGTTCTTTCTTTTACCCTTTTTCTGTTTTCTCTTTTTTCTTCTTGTCACCCCCCACTTTTTTACCATGTTTGTATAATGATGATATCTTTTAAGTTAAATTTAAGTATTATTACACCATCCATCTTTAGAATGTTAATCAATTCAAACTCGATTTTATAGCATAAATCTCTATCTCTATCTTCCTGATCGACGGTTGATTTCTCTAGCTTAACTACTAATTAATTCGAAGTAAGAGACAAAATTGCTTTAAATACACACCTGAATCCAAATCTCCACCATCAACTGAAGAGGGGATTGAATGCTGTGGGAAAAAAGAG

mRNA sequence

GTGGATGGCTTTGGTAGAAGCAAGGACCAAGTTGATACACGTGGTGAGAGGTAAGTGGATGAACAATTTAGAACGGATCCCACAAGGAGTAAGAACATCCAATCATCATCTGAGTCAGCGAAACGTTGAATAACGAACCGATTTTACCAAAGAACCGGTCAACGACCATAGGGTAAAGCTCAAAATGGTCCTCATGAGAAAGGGTATTTCAGTCTTTTCAACTACCGTCCAATCTCAACACCGTTTCCCTAAACCTTTAAACGAACCGAACGCATCGCTCAGATTTCTTCATTTTGGAGAGTTTGAATTTTTGTTTTTTTTTTTTGAAATTTTTTGTTTCTCATCAAACTGCAAATGAAGAATCGAATATCAGCGAAGCCATGGATTCTCGGATTGGCCGTCGCCGGCTTAATCGCGGGTTCTTTATTGATTTCCGGTTACATAGGATTTGTGGACAGCAACTTATTCTGCTCAATCTCCCGCACCGGAGCACCTACAATAACCGGGTATTCACCGGCGGAAGTCCAGTTTCAGTCTATAATCCATTACGCAACCTCGAAAACAGTTCCACAACAATCAATCGACGAGATCCATATCTCATACGACGTATTGAAGGTACGATGTCCTTGCAACTTCCTTGTTTTCGGGCTCGGCCACGATTCCTTGATGTGGGCGTCGCTTAACCCACTTGGAACTACGATTTTTCTCGAAGAAGATCCGAAATGGGTTCAGACGGTTCTTAAAGACGCTCCGATGCTTCGAGCTTATCACGTCCAGTATCGGACGCAGCTTCAGGAAGCAGATCGGTTGCTCTCAACCTACAAATCAGAGCCATATTGTTCGCCGTCGAAGGCATTTCTTAAGGGAAATGAAAAGTGTAAGTTAGCGCTTCATAATCTCCCAGAAGAGATTTACGAGAAGGAATGGGACCTGATAATGATCGACGCGCCAAGAGGGTATTTTGCGGAGGCTCCTGGTCGGATGGCGGCGATATTCTCCGCCACCGTGATGGCAAGGAATAGGAAAGGATCCGGCGTTACTGATGTGTTTTTGCACGACGTTGATCGGAAAGTGGAGAGAACATTTGCACAGGAGTTTTTGTGTAAAAATAAGTATTATGTAAACGGAGTTGGAAGGTTATGGCATTTCAAGATTCCTCCGGCGGCCAATATGAGCGAAGCCGATCGATCTTCCGATCGATTTTGTTAGATCAGAAGGGATTTGTTCACCGGAAATTATTTTTGTCTAAAGATAGTTATCGGTCTAGGGACCGGCGGTGAGGGAGTTGAAGGGAAAATTTTCTTTGGATATCAATAAAAAACCGCCGGAGTTTGAGCTCATGGGTCGGACGGACAATTTTGGCCGTCAACTCCGGCTGTGTTTTCGGCCGAAAAAAAAGTAGAAGATTTCAATCTTCTCCTTAATTGACTTTTTGTTCTTTCTTTTACCCTTTTTCTGTTTTCTCTTTTTTCTTCTTGTCACCCCCCACTTTTTTACCATGTTTGTATAATGATGATATCTTTTAAGTTAAATTTAAGTATTATTACACCATCCATCTTTAGAATGTTAATCAATTCAAACTCGATTTTATAGCATAAATCTCTATCTCTATCTTCCTGATCGACGGTTGATTTCTCTAGCTTAACTACTAATTAATTCGAAGTAAGAGACAAAATTGCTTTAAATACACACCTGAATCCAAATCTCCACCATCAACTGAAGAGGGGATTGAATGCTGTGGGAAAAAAGAG

Coding sequence (CDS)

ATGAAGAATCGAATATCAGCGAAGCCATGGATTCTCGGATTGGCCGTCGCCGGCTTAATCGCGGGTTCTTTATTGATTTCCGGTTACATAGGATTTGTGGACAGCAACTTATTCTGCTCAATCTCCCGCACCGGAGCACCTACAATAACCGGGTATTCACCGGCGGAAGTCCAGTTTCAGTCTATAATCCATTACGCAACCTCGAAAACAGTTCCACAACAATCAATCGACGAGATCCATATCTCATACGACGTATTGAAGGTACGATGTCCTTGCAACTTCCTTGTTTTCGGGCTCGGCCACGATTCCTTGATGTGGGCGTCGCTTAACCCACTTGGAACTACGATTTTTCTCGAAGAAGATCCGAAATGGGTTCAGACGGTTCTTAAAGACGCTCCGATGCTTCGAGCTTATCACGTCCAGTATCGGACGCAGCTTCAGGAAGCAGATCGGTTGCTCTCAACCTACAAATCAGAGCCATATTGTTCGCCGTCGAAGGCATTTCTTAAGGGAAATGAAAAGTGTAAGTTAGCGCTTCATAATCTCCCAGAAGAGATTTACGAGAAGGAATGGGACCTGATAATGATCGACGCGCCAAGAGGGTATTTTGCGGAGGCTCCTGGTCGGATGGCGGCGATATTCTCCGCCACCGTGATGGCAAGGAATAGGAAAGGATCCGGCGTTACTGATGTGTTTTTGCACGACGTTGATCGGAAAGTGGAGAGAACATTTGCACAGGAGTTTTTGTGTAAAAATAAGTATTATGTAAACGGAGTTGGAAGGTTATGGCATTTCAAGATTCCTCCGGCGGCCAATATGAGCGAAGCCGATCGATCTTCCGATCGATTTTGTTAG

Protein sequence

MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQSIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEEDPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALHNLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKVERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC*
Homology
BLAST of CSPI05G21370 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.5e-97
Identity = 173/284 (60.92%), Postives = 214/284 (75.35%), Query Frame = 0

Query: 6   SAKPWIL-GLAVAGLIAGSLLISGYIGFVDSNLF-CSISRTGAPTITG---YSPAEVQFQ 65
           S K WI+ G+ +AGL+ G+LL + +I   D  LF CS +   +  +     Y    +Q Q
Sbjct: 7   SEKRWIITGVLLAGLVGGALLFTSFIRAADETLFLCSTASAKSRAVAAAADYEATPIQLQ 66

Query: 66  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 125
           +I+HYATS  VPQQ++ EI IS+++LK   P NFLVFGLG DSLMWASLNP G T+FLEE
Sbjct: 67  AIVHYATSNVVPQQNLAEISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEE 126

Query: 126 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 185
           D +W Q V KD+P LRA+HV+YRTQLQ+AD LL +YK+EP C P+K++L+GNEKCKLAL 
Sbjct: 127 DLEWFQKVTKDSPFLRAHHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKCKLALT 186

Query: 186 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 245
            LP+E Y+ EWDL+M+DAP+GYFAEAPGRMAAIFSA VMARNRK  GVT VFLHDV+R+V
Sbjct: 187 GLPDEFYDTEWDLLMVDAPKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNRRV 246

Query: 246 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           E+TFA+EFLC+ KY VN  GRLWHF IPPAA  +  D    RFC
Sbjct: 247 EKTFAEEFLCR-KYRVNAAGRLWHFAIPPAAANATIDSGDYRFC 289

BLAST of CSPI05G21370 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.3e-58
Identity = 117/228 (51.32%), Postives = 156/228 (68.42%), Query Frame = 0

Query: 60  QSIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLE 119
           Q++IHY+TS   PQQ++ EI +S  VL  + PCNFLVFGLGHDSLMW+SLN  G T+FLE
Sbjct: 62  QALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYGGRTVFLE 121

Query: 120 EDPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEK---CK 179
           ED  W++ + +  PML +YHV Y +++ +AD L+   K  P C+       G+ +   C+
Sbjct: 122 EDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVGKG-PECTAI-----GDPRYSMCQ 181

Query: 180 LALHNLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDV 239
           LAL  LP EIYE  WDLIM+DAP GY+ EAPGRM AI++A +MARNRK  G TDVF+HDV
Sbjct: 182 LALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVHDV 241

Query: 240 DRKVERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           +R++E  F++ FLC+  Y     GRL HF IP   + SE++ S+  FC
Sbjct: 242 NREIEDKFSKAFLCEG-YMKKQEGRLRHFIIPSYRDGSESE-SNRPFC 281

BLAST of CSPI05G21370 vs. ExPASy Swiss-Prot
Match: Q9LQ32 (Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 9.5e-57
Identity = 114/224 (50.89%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           +++HY TS   PQQ+ DE+ +S  VL  + PCNFLVFGLGHDSLMWASLN  G T+F+EE
Sbjct: 76  ALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFIEE 135

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           D  W+  V K  P L +YHV Y T+++++D+L+   +SE   S S      N KC LAL 
Sbjct: 136 DQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELGRSEECRSVSD---PRNSKCDLALK 195

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           + P + YE +WDLIM+DAP GY  EAPGRM+AI++A ++ARNR+  G TDVF+HDV+R V
Sbjct: 196 DFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNRE-DGETDVFVHDVNRPV 255

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           E  F+  FLCK  Y     GRL HF IP     S   R+   FC
Sbjct: 256 EDEFSATFLCKG-YMREQNGRLRHFTIP-----SHRARAGRPFC 289

BLAST of CSPI05G21370 vs. ExPASy Swiss-Prot
Match: Q9T0F7 (Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-55
Identity = 104/208 (50.00%), Postives = 143/208 (68.75%), Query Frame = 0

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           +++HY TS+  PQQ+ DE+ +S  VL  + PCNFLVFGLGHDSLMWASLN  G T+FLEE
Sbjct: 68  ALVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFLEE 127

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           D  W++TV K  P L +YHV Y T+++++++L+   ++E   + S      + KC L+L 
Sbjct: 128 DEAWIETVTKKFPNLESYHVVYDTKVKDSNKLMELKRTEDCKAVSD---PRDSKCALSLK 187

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
             P ++YE +WD+IM+DAP GY  EAPGRM+AI++A ++ARNR   G TDVF+HD++R V
Sbjct: 188 GFPADVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVFVHDINRPV 247

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIP 269
           E  F+  FLC   Y     GRL HF IP
Sbjct: 248 EDEFSVAFLC-GGYMKEQQGRLRHFNIP 271

BLAST of CSPI05G21370 vs. ExPASy Swiss-Prot
Match: Q9FH92 (Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.4e-36
Identity = 91/236 (38.56%), Postives = 137/236 (58.05%), Query Frame = 0

Query: 61  SIIHYATSKTVPQQSIDEIHISY-------DVLKVRC--PCNFLVFGLGHDSLMWASLNP 120
           +++HYA+      +S D  H+SY       DVL+ RC  PCN LVFGL H++L+W SLN 
Sbjct: 84  AMLHYAS------RSNDSYHMSYGEMKSISDVLR-RCSPPCNLLVFGLTHETLLWKSLNH 143

Query: 121 LGTTIFLEEDPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYK--SEPYCSPSKAFL 180
            G T+F+EE+  +     +  P +  + VQY T+ +EA  L+S  K  +   C P +  L
Sbjct: 144 NGRTVFIEENRYYAAYFEEIHPEIEVFDVQYTTKAREARELVSAVKEAARNECRPVQNLL 203

Query: 181 KGNEKCKLALHNLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSG-V 240
             +  CKL L++LP  +Y+ +WD+I++D PRG   + PGRM++IF+A V+AR++KG    
Sbjct: 204 FSD--CKLGLNDLPNHVYDVDWDVILVDGPRGDGGDVPGRMSSIFTAAVLARSKKGGNPK 263

Query: 241 TDVFLHDVDRKVERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           T VF+HD  R VER    EFLC+    V     L H+       + + D++S +FC
Sbjct: 264 THVFVHDYYRDVERLCGDEFLCREN-LVESNDLLAHYV------LEKMDKNSTQFC 303

BLAST of CSPI05G21370 vs. ExPASy TrEMBL
Match: A0A0A0KTC1 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589350 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.4e-164
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ
Sbjct: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 284

BLAST of CSPI05G21370 vs. ExPASy TrEMBL
Match: A0A5A7ST91 (Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002300 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.5e-161
Identity = 280/284 (98.59%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSIS TGAP ITGYSPAEVQFQ
Sbjct: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISGTGAPIITGYSPAEVQFQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSP+KAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPTKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 284

BLAST of CSPI05G21370 vs. ExPASy TrEMBL
Match: A0A1S3BEX8 (probable methyltransferase At1g27930 OS=Cucumis melo OX=3656 GN=LOC103488882 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.5e-161
Identity = 280/284 (98.59%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSIS TGAP ITGYSPAEVQFQ
Sbjct: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISGTGAPIITGYSPAEVQFQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSP+KAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPTKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 284

BLAST of CSPI05G21370 vs. ExPASy TrEMBL
Match: A0A6J1GW49 (probable methyltransferase At1g27930 OS=Cucurbita moschata OX=3662 GN=LOC111458130 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 1.9e-153
Identity = 265/284 (93.31%), Postives = 271/284 (95.42%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNR+SA PWILGLA+AGLIAGSL+ISGYIGFVDSNLFCSIS   APTITGYS AE+Q Q
Sbjct: 1   MKNRLSATPWILGLAIAGLIAGSLMISGYIGFVDSNLFCSISGIRAPTITGYSAAEIQLQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSK VPQQS DEI IS+DVLK RCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKIVPQQSFDEISISFDVLKARCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHD+DRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDIDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMS ADR SDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSSADRISDRFC 284

BLAST of CSPI05G21370 vs. ExPASy TrEMBL
Match: A0A6J1KEA2 (probable methyltransferase At1g27930 OS=Cucurbita maxima OX=3661 GN=LOC111493053 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.3e-153
Identity = 265/284 (93.31%), Postives = 270/284 (95.07%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNR SA PWILGLA+AGLIAGSL+ISGYIGFVDSNLFCSIS   APTITGYS AE+Q Q
Sbjct: 1   MKNRFSATPWILGLAIAGLIAGSLMISGYIGFVDSNLFCSISGIRAPTITGYSAAEIQLQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSK VPQQS DEI IS+DVLK RCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKIVPQQSFDEISISFDVLKTRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHD+DRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDIDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMS ADR SDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSSADRISDRFC 284

BLAST of CSPI05G21370 vs. NCBI nr
Match: XP_011655557.1 (probable methyltransferase At1g27930 [Cucumis sativus] >KGN51667.1 hypothetical protein Csa_008102 [Cucumis sativus])

HSP 1 Score: 586.6 bits (1511), Expect = 1.1e-163
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ
Sbjct: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 284

BLAST of CSPI05G21370 vs. NCBI nr
Match: XP_008446029.1 (PREDICTED: probable methyltransferase At1g27930 [Cucumis melo] >KAA0034190.1 putative methyltransferase [Cucumis melo var. makuwa] >TYK15731.1 putative methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 578.6 bits (1490), Expect = 3.0e-161
Identity = 280/284 (98.59%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSIS TGAP ITGYSPAEVQFQ
Sbjct: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISGTGAPIITGYSPAEVQFQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSP+KAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPTKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 284

BLAST of CSPI05G21370 vs. NCBI nr
Match: XP_038891342.1 (probable methyltransferase At1g27930 [Benincasa hispida])

HSP 1 Score: 572.4 bits (1474), Expect = 2.2e-159
Identity = 273/284 (96.13%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNR+SAKPWILGLA+AGLIAGSLLISGYIGFVDSNLFCSIS TGAPTITGYSPA++Q Q
Sbjct: 1   MKNRLSAKPWILGLAIAGLIAGSLLISGYIGFVDSNLFCSISGTGAPTITGYSPADIQLQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSKTVPQQS+DEIHIS+DVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKTVPQQSLDEIHISFDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSP+KAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPTKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHDVDRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDVDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADR+SDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRTSDRFC 284

BLAST of CSPI05G21370 vs. NCBI nr
Match: KAG6601603.1 (putative methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.0 bits (1421), Expect = 3.1e-153
Identity = 266/284 (93.66%), Postives = 271/284 (95.42%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNR+SA PWILGLAVAGLIAGSL+ISGYIGFVDSNLFCSIS   APTITGYS AE+Q Q
Sbjct: 1   MKNRLSATPWILGLAVAGLIAGSLMISGYIGFVDSNLFCSISGIRAPTITGYSAAEIQLQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSK VPQQS DEI IS+DVLK RCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKIVPQQSFDEISISFDVLKARCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHD+DRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDIDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMS ADR SDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSSADRISDRFC 284

BLAST of CSPI05G21370 vs. NCBI nr
Match: XP_022956372.1 (probable methyltransferase At1g27930 [Cucurbita moschata])

HSP 1 Score: 551.6 bits (1420), Expect = 4.0e-153
Identity = 265/284 (93.31%), Postives = 271/284 (95.42%), Query Frame = 0

Query: 1   MKNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNLFCSISRTGAPTITGYSPAEVQFQ 60
           MKNR+SA PWILGLA+AGLIAGSL+ISGYIGFVDSNLFCSIS   APTITGYS AE+Q Q
Sbjct: 1   MKNRLSATPWILGLAIAGLIAGSLMISGYIGFVDSNLFCSISGIRAPTITGYSAAEIQLQ 60

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           SIIHYATSK VPQQS DEI IS+DVLK RCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE
Sbjct: 61  SIIHYATSKIVPQQSFDEISISFDVLKARCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH
Sbjct: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSA VMARNRKGSGVTDVFLHD+DRKV
Sbjct: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSAAVMARNRKGSGVTDVFLHDIDRKV 240

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMS ADR SDRFC
Sbjct: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSSADRISDRFC 284

BLAST of CSPI05G21370 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 382.1 bits (980), Expect = 3.9e-106
Identity = 186/285 (65.26%), Postives = 224/285 (78.60%), Query Frame = 0

Query: 2   KNRISAKPWILGLAVAGLIAGSLLISGYIGFVDSNL-FCSISRTGAPTITGYSPAEVQFQ 61
           K  +  +PW L +A+AGLI G++LI+ +I   D+ L  CS ++  A +I  Y+   +Q Q
Sbjct: 10  KQTLLERPWFLAVALAGLIGGAMLITSFIRATDNTLSLCSTAKNTAASIAKYTATPIQLQ 69

Query: 62  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 121
           SI+HYATS TVPQQS +EI IS +VLK R PCNFLVFGLG DSLMWASLNP GTT+FLEE
Sbjct: 70  SIVHYATSHTVPQQSFEEISISLNVLKERLPCNFLVFGLGRDSLMWASLNPGGTTVFLEE 129

Query: 122 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAF-LKGNEKCKLAL 181
           DP+W++ VLKDAP LRA+HVQYRT L EA RLLSTYK+EP C P+KAF ++ NEKC LAL
Sbjct: 130 DPEWIEAVLKDAPSLRAHHVQYRTHLSEAGRLLSTYKNEPMCLPAKAFPIRYNEKCPLAL 189

Query: 182 HNLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRK 241
            +LP+E Y+ EWDLIM+DAP+GYF EAPGRMAAIFS+ +MARNRKG G T VFLHDV+RK
Sbjct: 190 TSLPDEFYDTEWDLIMVDAPKGYFPEAPGRMAAIFSSAIMARNRKGDGTTHVFLHDVNRK 249

Query: 242 VERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           VE  FA EFLC+ KY VN VGRLWHF+IP AANM+  D+  DRFC
Sbjct: 250 VENAFANEFLCE-KYKVNSVGRLWHFEIPNAANMT--DQPGDRFC 291

BLAST of CSPI05G21370 vs. TAIR 10
Match: AT1G27930.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 355.5 bits (911), Expect = 3.9e-98
Identity = 173/284 (60.92%), Postives = 214/284 (75.35%), Query Frame = 0

Query: 6   SAKPWIL-GLAVAGLIAGSLLISGYIGFVDSNLF-CSISRTGAPTITG---YSPAEVQFQ 65
           S K WI+ G+ +AGL+ G+LL + +I   D  LF CS +   +  +     Y    +Q Q
Sbjct: 7   SEKRWIITGVLLAGLVGGALLFTSFIRAADETLFLCSTASAKSRAVAAAADYEATPIQLQ 66

Query: 66  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 125
           +I+HYATS  VPQQ++ EI IS+++LK   P NFLVFGLG DSLMWASLNP G T+FLEE
Sbjct: 67  AIVHYATSNVVPQQNLAEISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEE 126

Query: 126 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 185
           D +W Q V KD+P LRA+HV+YRTQLQ+AD LL +YK+EP C P+K++L+GNEKCKLAL 
Sbjct: 127 DLEWFQKVTKDSPFLRAHHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKCKLALT 186

Query: 186 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 245
            LP+E Y+ EWDL+M+DAP+GYFAEAPGRMAAIFSA VMARNRK  GVT VFLHDV+R+V
Sbjct: 187 GLPDEFYDTEWDLLMVDAPKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNRRV 246

Query: 246 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           E+TFA+EFLC+ KY VN  GRLWHF IPPAA  +  D    RFC
Sbjct: 247 EKTFAEEFLCR-KYRVNAAGRLWHFAIPPAAANATIDSGDYRFC 289

BLAST of CSPI05G21370 vs. TAIR 10
Match: AT1G09610.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 227.3 bits (578), Expect = 1.6e-59
Identity = 117/228 (51.32%), Postives = 156/228 (68.42%), Query Frame = 0

Query: 60  QSIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLE 119
           Q++IHY+TS   PQQ++ EI +S  VL  + PCNFLVFGLGHDSLMW+SLN  G T+FLE
Sbjct: 62  QALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYGGRTVFLE 121

Query: 120 EDPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEK---CK 179
           ED  W++ + +  PML +YHV Y +++ +AD L+   K  P C+       G+ +   C+
Sbjct: 122 EDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVGKG-PECTAI-----GDPRYSMCQ 181

Query: 180 LALHNLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDV 239
           LAL  LP EIYE  WDLIM+DAP GY+ EAPGRM AI++A +MARNRK  G TDVF+HDV
Sbjct: 182 LALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVHDV 241

Query: 240 DRKVERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           +R++E  F++ FLC+  Y     GRL HF IP   + SE++ S+  FC
Sbjct: 242 NREIEDKFSKAFLCEG-YMKKQEGRLRHFIIPSYRDGSESE-SNRPFC 281

BLAST of CSPI05G21370 vs. TAIR 10
Match: AT1G33800.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 221.9 bits (564), Expect = 6.7e-58
Identity = 114/224 (50.89%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           +++HY TS   PQQ+ DE+ +S  VL  + PCNFLVFGLGHDSLMWASLN  G T+F+EE
Sbjct: 76  ALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFIEE 135

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           D  W+  V K  P L +YHV Y T+++++D+L+   +SE   S S      N KC LAL 
Sbjct: 136 DQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELGRSEECRSVSD---PRNSKCDLALK 195

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKGSGVTDVFLHDVDRKV 240
           + P + YE +WDLIM+DAP GY  EAPGRM+AI++A ++ARNR+  G TDVF+HDV+R V
Sbjct: 196 DFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNRE-DGETDVFVHDVNRPV 255

Query: 241 ERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           E  F+  FLCK  Y     GRL HF IP     S   R+   FC
Sbjct: 256 EDEFSATFLCKG-YMREQNGRLRHFTIP-----SHRARAGRPFC 289

BLAST of CSPI05G21370 vs. TAIR 10
Match: AT1G71690.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-57
Identity = 108/225 (48.00%), Postives = 150/225 (66.67%), Query Frame = 0

Query: 61  SIIHYATSKTVPQQSIDEIHISYDVLKVRCPCNFLVFGLGHDSLMWASLNPLGTTIFLEE 120
           +++HYA+S   PQQ++ EI ++   L+ + PCNFLVFGLGHDSLMWA+LN  G TIFL+E
Sbjct: 75  ALVHYASSNVTPQQTLSEISVTKKELEKKSPCNFLVFGLGHDSLMWATLNHGGRTIFLDE 134

Query: 121 DPKWVQTVLKDAPMLRAYHVQYRTQLQEADRLLSTYKSEPYCSPSKAFLKGNEKCKLALH 180
           D  W+  + +  P L +YHV+Y+T++++A+ L++  K    C      L+ +  C+LAL 
Sbjct: 135 DESWIHQIAEKFPSLESYHVRYKTKVRDAEALMAATKDREECRRVSTDLRVS-TCELALK 194

Query: 181 NLPEEIYEKEWDLIMIDAPRGYFAEAPGRMAAIFSATVMARNRKG-SGVTDVFLHDVDRK 240
            LPE +YE EWDLIM+DAP G+  EAPGRM+AI++A ++AR RK     T VF+HDVDRK
Sbjct: 195 GLPEVVYETEWDLIMVDAPTGFHEEAPGRMSAIYTAGMIARRRKDEEETTAVFVHDVDRK 254

Query: 241 VERTFAQEFLCKNKYYVNGVGRLWHFKIPPAANMSEADRSSDRFC 285
           VE  F+ EFLC++ Y     GRL HF +P   N      S  +FC
Sbjct: 255 VEDEFSMEFLCRD-YMTKQEGRLRHFTVPSHRNYGV---SGVKFC 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7F95.5e-9760.92Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g2793... [more]
Q6NMK12.3e-5851.32Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 P... [more]
Q9LQ329.5e-5750.89Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 P... [more]
Q9T0F71.8e-5550.00Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 P... [more]
Q9FH921.4e-3638.56Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTC15.4e-164100.00Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G58... [more]
A0A5A7ST911.5e-16198.59Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BEX81.5e-16198.59probable methyltransferase At1g27930 OS=Cucumis melo OX=3656 GN=LOC103488882 PE=... [more]
A0A6J1GW491.9e-15393.31probable methyltransferase At1g27930 OS=Cucurbita moschata OX=3662 GN=LOC1114581... [more]
A0A6J1KEA23.3e-15393.31probable methyltransferase At1g27930 OS=Cucurbita maxima OX=3661 GN=LOC111493053... [more]
Match NameE-valueIdentityDescription
XP_011655557.11.1e-163100.00probable methyltransferase At1g27930 [Cucumis sativus] >KGN51667.1 hypothetical ... [more]
XP_008446029.13.0e-16198.59PREDICTED: probable methyltransferase At1g27930 [Cucumis melo] >KAA0034190.1 put... [more]
XP_038891342.12.2e-15996.13probable methyltransferase At1g27930 [Benincasa hispida][more]
KAG6601603.13.1e-15393.66putative methyltransferase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022956372.14.0e-15393.31probable methyltransferase At1g27930 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G67330.13.9e-10665.26Protein of unknown function (DUF579) [more]
AT1G27930.13.9e-9860.92Protein of unknown function (DUF579) [more]
AT1G09610.11.6e-5951.32Protein of unknown function (DUF579) [more]
AT1G33800.16.7e-5850.89Protein of unknown function (DUF579) [more]
AT1G71690.11.5e-5748.00Protein of unknown function (DUF579) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006514IRX15/IRX15L/IGXMTIGRFAMTIGR01627TIGR01627coord: 60..276
e-value: 9.8E-77
score: 255.6
IPR006514IRX15/IRX15L/IGXMPANTHERPTHR31444OS11G0490100 PROTEINcoord: 2..284
IPR021148Polysaccharide biosynthesis domainPFAMPF04669Polysacc_synt_4coord: 78..265
e-value: 3.9E-72
score: 241.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 66..279
e-value: 8.8E-6
score: 27.3
NoneNo IPR availablePANTHERPTHR31444:SF38BNAC02G45640D PROTEINcoord: 2..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G21370.1CSPI05G21370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0009834 plant-type secondary cell wall biogenesis
biological_process GO:0045492 xylan biosynthetic process
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity