CSPI05G21220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G21220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein EI24 homolog
LocationChr5: 22041223 .. 22046610 (-)
RNA-Seq ExpressionCSPI05G21220
SyntenyCSPI05G21220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGTTTTGGCGGCTGCCTTCAATGGGAAGCGAGCGAACTATATAGTTAATCTCCAAACTGTCTTTCTCCGGAGACATTGCGGCATTTTAACCATGGATTCTGCTGCCCACGCGTTGATCAAGGTCGTAGGTGAAAAATCAAGGAGGGCTTCTCAATTATGGCTTGAAGGTTTTATTGATGCTTGCTGTCTTCACCGTGTTATCATCCTCTGTCGCAGGTTGAACTTCTATTTTTAACTTCACTCTCTGTTTTTCTATCACTGTGAATACTGGACTGGGAGGATTGTACGAACAATTCTGAATTATGATTTATTTTTTTGTTCTGGGTATTTTTATAATTTCATCTGAAATTGAATTTCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTAAATGGGTTAATCTTCTTAGGAAGGTAATTGTTCTCCGGTTTCTTATCTCTCTCTCTGCTCTGCGTTATTTTTGTTGATTCTGCTGTAATTACGCTATTTAACAATCTTATTTACTACCTTTATCCGCCTACGAGATTCCAACTTGTGTTAGGAGTGCAAATGAAGTTTCACATTGGTTAGGGAAGGTATTGGTTATTGGTGTATCTTAAGATTAGAGCAATTATCTCTGTTGGTATGAAGTATTTCGAAGTCGTACTGTTATGGGGATAGGTGTAGGTTATGTTATTCTTATCAGATTGTTGTAATTAGTCCTAATTGGATAGTATGGCCTAGTTTATGTTAGCAATTTGCATTCTTTCTGTCCCCACATTTGAACGAACTACGAAGCTAGATGATTTGAAATTTATAATCTCACTATGTTTCTTTGATTGATATGGAAATTTGCTTGTATCTTTGTCAATTTGTCCACTTAAGATGATGCTTCATAAGATAATTTATAAGTCTTGGATCTAATAAAAACTAAAATTTGTCTCTATGTCAGTGGTCTAAATGAACTGACTTAGGAACCTGCTTCTGGACTCTTTCCTTAAAAGTTTCTCTCCTCCCCTCTCTCTTTCTAGGGCATGGGGGAGATCTTCACATTCTTGTGTCTTGTGGATTATGGTTTTGTTTTGTATAATATAACCTCATGATATATTCAAGACACCTTAATTTGTTCAGAGATTATTTCAATGTCATTAAAATTTTGTCAGCACTTTCCCTGTATCATTTTTTTTATATACAATAATGTCAAACAATGAAAAGTCAGCATGTTTCTTGATTTTCTTTTTTGCAGTCTTTTGATCCTAAACTCAGTTATCATCCCAACACTACAATGGATATTACCTGATCAGCAATCTGTGATTAATTCTCACAAGTTGAACGCATTTGGTGCTACTTTAAAATTATATTCCTTCTTACGTCTTGGACTGATACAACTTTTTTATGTAAGTACGAATTTGATGCCGCGTTTATAATTGTGGAACAAATTAAGTTGTTTTCTTTCTGCATCACATAGAGGCCCTCTTTGATAAGCATTTGAGTATTGATTTTTAGCTTTTGAAAATTATGCCTACAAACACCTTTTCCACCTCTAAATTTTGTGTTGAGTTTTATGATGTCTACCATGTTTTTAAATTCCAAAAACCAAATGGTTACCAAACGGGCATTAGATTTTCCAACATTTGCTGTCAAAATTAATAGTAGGAAAAGAGAACCACGTTTTAAATTACGCTTATGAAATTTTCTTTTTGTAAGCTTAGGAATATTCTTATGGATTATAATATTGGTGTTTGACAAGACAATTTCTTTGCTGTTTCAATACGACTGCAAACTATGATTGTTGTACTCAACCAGTTTATATGTAATGACTAAAACGATTTACAAAAGAACCTTGAATAACATATTTATTATTGTGTTTGTTTCAATTATTTTTACTGACTGATTGTTTTTCTTAATATTGCTTGCAGTTGTTTTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTATTTCTTTCCCCTCTATTTTCTTTAACATTTTCGCATTTTTTTTTAATCAATTTTGCCTAACTTTGGTCGAAGTGTTTTCTATTACTTATGAACTCCTCAAACCACATTGTTTAATGTTTGAGTTTTTAGTTGCATAATAAGTTTAGATATAACATTTATTCTGTTAACTTTTACATTTAAAGTTTGGACATATGTCGTTATACTCATTACATTTGGATGGAAGTTGTTTTCCCAAAGTAACTATTTGTTGTTCTGAAGTTTCTAAAAAGTTTCTATCTGATCCAGGTACAACGATATTGCTAAACATGGGTTTGCTGCAATGGAACAATCTGAGCCAACAGCAGCAAACCCTTCAACACAAAATGAAGGGTCTTTCAATACTGCTCAAACTGGAAAATCAATTGGATTGGGGAGGTAGGTTTTTAATATGGTTATTGGTGATTTCTGAAATGTTCTTGGCTGCTTGTTCGATGCACTTTTCTGCTTGATACTTGTTGACCTAAAATTGTTCTCAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTTTACTTCTGATCAACTCCTTCTTCCTTGAGGTAATAGCATTGGAAATTAGTTTTACACTTTTGCTCTTATGTTTTCTTTTCTACTTCACATGGTTATATGTGCTTATCTTTCTTTTACTTTAAGCACTTTTAGATGCTGATTTGCTTATTTACAGCTATATACGCACATCACATTCTTCTACTTCTATCCAATTTCTAAGTCTTGTTTTCTTATACATGTAGGTCTATGCTACTGGTTTCTTGCCTTTAGTGGGAAAGACACTCAATTTTCTGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAGTATGTAGTTCTTTCCCTTATAAGTTTTGATGTCTATTGACCATTCACTTGACGAACTATTCAATTCAATTTTTTAAAAAAACTTGTAGATACAAGTGGAACCTTTCAGATGTGAGTCTTGATAGGAGGTTGGACTTTTTTGAGTCTAACTGGGCTTTCTTTGCTGGTTTCGGTAAGATTACATGACTTGATCAATTTATTTAGTTTAGTTTCTATATTTTGCTAATTTTGATTGTGAAGATTAATGAGAAAAATCTACTGTTAAATTAATTCAATCCAATGCAGCTCCATATTATTCCGATGTTGATTATAAAGATTAGTTTGTCTGTTTTTCAGTGAGATGACTATTTAGTTAGTTTGATAGAAGAAGTATTGAGTTTGATAATTATGATCAGAATACAATAAAACAAAAATGTGAACATGAGCATAACACAACTATACATAAAGACATATGTTTGAATCCTCACCTCCACATCGTCGTACTAAAAAAGAAATTACTATAAATTGTGGAGGGAGGGTGGGAAGAATTAAGAGTTCTGATATCAAGTGTTGCTCAAGGGATCGTTTTCCAGTTCCCTATTACCTTGATTTACCCATATAATACCATAATTTCCGTACAGTTCAATGTTTCTTTATTCTTGGCTTGTCTCCCTACATATGTTAAGACAGCCACGATTATAATTCGCAAGTGGGATATCTTGGCCCATGGCAGTAACATATAACTCTTTGGTTGACAAAATTCGTACAAATGTGAGCAATGGCTCCTTAAGCAAATATCAACAAGTATTTGTTGACGTTAGATTCTCTCATGACATTTTGCTTTTATTTCATATTTTGGAGTTTGGCTTTTTTGCACTTGACTGCTGTGTCTCTGATTATTAATTGAAATTTGAGCTCGTTTCTCTTTTCTTGTCATGTTATCATTACGTAGTTGTGAATTGTAGACAATATGATATACTTTTGAGAAGTTTGAGGCATGCCAGTTTAATTGTCTTTCCAGGTAGTCCTTGCGTTCTTGCTATATTTTTCTTCTCACCACTTGTGAGCTATGCAGTAATGGCTATCTTATTTCCACTGGTAAATTGAAACATTTTCCTTCATTCCATCTCTTCACACACACACACACAAACTCAATTCCTTTCAAATATGTAACAATGTTGTGTTTCCTGAAATACTCACTTCTTACTCTTCCTGTTGCTTTTCATCCTATGTACCTGTCAGTTTGTTCTGACAGCCACAGGCTCAGTTGCCGAGCAGGATATTTATTCACAAAGAGCAACTTGGAAATGTGCAGGACTGGGGAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGTAAGTTACCTTTCTTCACAGTTTACCCTAAACTTGTATTTGCAATCAATAATTTATTGCGATCTGTATAGAATAGAAGTTGATTAAATGAGACAAGAAAAAATGTTTTTGATGTTAAACAAATGTTGCAAAAATCTCTCTGGAATTGTAATTGGCGGGAAAAACAGCAGCTATCTAAAAGATGGACAAAAGAAAGAACATAAAGATCATAGATAGAGGTTATGACTTTGTGCTATGTATTATAGGTATTTCTAGAAAGCTGCAAATGGACAAATTAAATAGAAATTCATTAGTAGTACAACAAATTGTAGAGAGTGAACGGTGAACCACGAATGGAAAACCTGAAAAACATTGACTATGAGATATTTTGAACTGTCCATGAAGTAAGGAAAGTCAAGTATAGCCTGCCAATGTTCTTTCCCCTTGTTGATTGACCTTGCAGGATGAAGCTCTTGACTCTCTTCCCTCACGAATCACAAGAAAAGATGCAGCAAAGCAAGGATCTATAACGAGCCTCGGAATCTGGGTCAATACACATACATTTTATGAACAGTAAGCCAAAATTCACTTGCCACTGCCCTCTATTCTGAACGTTTTCTGTTTGTTCATTTATTTTATAAAGCTGTCTGCCTACTGCATTGTCTCTCAACTATTAATGTCTAATGGAGCAGAACTCAAGTGGCTAAAAGTCAGATACTACAATCCCCCTTATCCACACAGAAACGATACAATAGGCGAAAACAAACCATAGCTCAACGCGTAGGCACAGTTCAGAAGATTGGTTGCTGTGTAAAATATCAAATAGGTAACAAAAGATTGGTACATTGCAATTCACGGGACGGATAGATAAGAATCTGTGAAGCTGAATGATAATATTGGTTTGGAAATCTATCCACCACTCTTTTGCTGTAACCTTGTTAAATTATGTACATAATCTATTTTTACTGGAAAGATTGTTCCAACTCTGTGTTTCACTTCCTTGTGTAAATACATGATTTAAGAATGGAATACTTCAAAGTTGGTAATGAGACGAAAGCTAAATTGATCCTGGGCATAATTGGAAAGAAGAAAAAGGTATTAAGATGATTTTGCTTATCATGTTTTGACATCTTTTACGTCTGCAGAGAGTTGAAAGGCATTTGCAAAGGCCAAAATTCAAGTGAGAGAAGAAAATAGTAGTAAGGTATGAATTTGATGAGTTTTCCTTTTTCTTTTCTTTTTTCTGT

mRNA sequence

GTCGTTTTGGCGGCTGCCTTCAATGGGAAGCGAGCGAACTATATAGTTAATCTCCAAACTGTCTTTCTCCGGAGACATTGCGGCATTTTAACCATGGATTCTGCTGCCCACGCGTTGATCAAGGTCGTAGGTGAAAAATCAAGGAGGGCTTCTCAATTATGGCTTGAAGGTTTTATTGATGCTTGCTGTCTTCACCGTGTTATCATCCTCTGTCGCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTAAATGGGTTAATCTTCTTAGGAAGTCTTTTGATCCTAAACTCAGTTATCATCCCAACACTACAATGGATATTACCTGATCAGCAATCTGTGATTAATTCTCACAAGTTGAACGCATTTGGTGCTACTTTAAAATTATATTCCTTCTTACGTCTTGGACTGATACAACTTTTTTATTTGTTTTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTACAACGATATTGCTAAACATGGGTTTGCTGCAATGGAACAATCTGAGCCAACAGCAGCAAACCCTTCAACACAAAATGAAGGGTCTTTCAATACTGCTCAAACTGGAAAATCAATTGGATTGGGGAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTTTACTTCTGATCAACTCCTTCTTCCTTGAGGTCTATGCTACTGGTTTCTTGCCTTTAGTGGGAAAGACACTCAATTTTCTGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAATACAAGTGGAACCTTTCAGATGTGAGTCTTGATAGGAGGTTGGACTTTTTTGAGTCTAACTGGGCTTTCTTTGCTGGTTTCGGTAGTCCTTGCGTTCTTGCTATATTTTTCTTCTCACCACTTGTGAGCTATGCAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACAGGCTCAGTTGCCGAGCAGGATATTTATTCACAAAGAGCAACTTGGAAATGTGCAGGACTGGGGAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCACGAATCACAAGAAAAGATGCAGCAAAGCAAGGATCTATAACGAGCCTCGGAATCTGGGTCAATACACATACATTTTATGAACAAACTCAAGTGGCTAAAAGTCAGATACTACAATCCCCCTTATCCACACAGAAACGATACAATAGGCGAAAACAAACCATAGCTCAACGCGTAGGCACAGTTCAGAAGATTGGTTGCTGTGTAAAATATCAAATAGGTAACAAAAGATTGGTACATTGCAATTCACGGGACGGATAGATAAGAATCTGTGAAGCTGAATGATAATATTGGTTTGGAAATCTATCCACCACTCTTTTGCTGTAACCTTGTTAAATTATGTACATAATCTATTTTTACTGGAAAGATTGTTCCAACTCTGTGTTTCACTTCCTTGTGTAAATACATGATTTAAGAATGGAATACTTCAAAGTTGGTAATGAGACGAAAGCTAAATTGATCCTGGGCATAATTGGAAAGAAGAAAAAGGTATTAAGATGATTTTGCTTATCATGTTTTGACATCTTTTACGTCTGCAGAGAGTTGAAAGGCATTTGCAAAGGCCAAAATTCAAGTGAGAGAAGAAAATAGTAGTAAGGTATGAATTTGATGAGTTTTCCTTTTTCTTTTCTTTTTTCTGT

Coding sequence (CDS)

ATGGATTCTGCTGCCCACGCGTTGATCAAGGTCGTAGGTGAAAAATCAAGGAGGGCTTCTCAATTATGGCTTGAAGGTTTTATTGATGCTTGCTGTCTTCACCGTGTTATCATCCTCTGTCGCAGGTCGAAAAAGCTTTTGATCCGGACGGGTCAGTGTTTTTTATTAAATGGGTTAATCTTCTTAGGAAGTCTTTTGATCCTAAACTCAGTTATCATCCCAACACTACAATGGATATTACCTGATCAGCAATCTGTGATTAATTCTCACAAGTTGAACGCATTTGGTGCTACTTTAAAATTATATTCCTTCTTACGTCTTGGACTGATACAACTTTTTTATTTGTTTTGGTTTTATCCAATGTACATTTTCAGCTTCATTCTGAGCAATATCTGGTACAACGATATTGCTAAACATGGGTTTGCTGCAATGGAACAATCTGAGCCAACAGCAGCAAACCCTTCAACACAAAATGAAGGGTCTTTCAATACTGCTCAAACTGGAAAATCAATTGGATTGGGGAGGGTTGTAATAGGAATAGGAGAGCAGCTATATTCTTTACTTCTGATCAACTCCTTCTTCCTTGAGGTCTATGCTACTGGTTTCTTGCCTTTAGTGGGAAAGACACTCAATTTTCTGCTTCTTTCTTGGATGTACGCCTACTATTGTTTTGAATACAAGTGGAACCTTTCAGATGTGAGTCTTGATAGGAGGTTGGACTTTTTTGAGTCTAACTGGGCTTTCTTTGCTGGTTTCGGTAGTCCTTGCGTTCTTGCTATATTTTTCTTCTCACCACTTGTGAGCTATGCAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACAGGCTCAGTTGCCGAGCAGGATATTTATTCACAAAGAGCAACTTGGAAATGTGCAGGACTGGGGAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCACGAATCACAAGAAAAGATGCAGCAAAGCAAGGATCTATAA

Protein sequence

MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL*
Homology
BLAST of CSPI05G21220 vs. ExPASy Swiss-Prot
Match: Q5BPL5 (Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.0e-110
Identity = 210/320 (65.62%), Postives = 249/320 (77.81%), Query Frame = 0

Query: 15  KSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVIIP 74
           KS++   LWLEGF +AC LHRV+ILC RS+KLL+RTGQCFLLNGLIFLGSL +    I P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 75  TLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYIFSFILSNIWYN 134
           +LQWILPDQ S + S +  ++G     Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 135 DIAKHGFAAMEQSEPTAANPSTQNE--GSFNTAQTGKSIGLGRVVIGIGEQLYSLLLINS 194
           DIAKHGF A+E S+  +A    Q E   S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 195 FFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFFESNWAFFAGF 254
           FFLEV   G +P +GK LNFLLLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 255 GSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYA 314
           GSPCVLAIFF SPLVS A+MAILFPLFVLTATGS  E+ I + R TWKCAGLG+LPIFY 
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 303

Query: 315 ANTLSMKLLTLF----PHES 329
           A+TLSM  L++F    PHE+
Sbjct: 304 ADTLSMLALSIFRLESPHEN 320

BLAST of CSPI05G21220 vs. ExPASy Swiss-Prot
Match: Q54PW9 (Protein EI24 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284253 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.4e-21
Identity = 84/304 (27.63%), Postives = 143/304 (47.04%), Query Frame = 0

Query: 22  LWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVIIPTLQWILP 81
           +WL G  D+  L   II   +S+ L      C  LNG+IFLG+ LI        L W+ P
Sbjct: 26  IWL-GVADSMKLKGAIIRTIKSEVLRKNFIHCIFLNGIIFLGTYLIY-------LYWVSP 85

Query: 82  DQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGF 141
                + ++ LN F     +++        +++  W YP+YIFS I ++ WY +IAK  F
Sbjct: 86  -----MLNYLLNHFPTLSNMFTI-------IYFSLWVYPVYIFSIIANSKWYTEIAKESF 145

Query: 142 AAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATG 201
                   T AN +     SF                   +++Y  LL     +      
Sbjct: 146 VI--SGRTTFANSTNGILSSF------------------VDEIYRNLLFGVILVMSAIIA 205

Query: 202 FLPLVGKTLNFLLLSWMYAYYCFEYKWNL-SDVSLDRRLDFFESNWAFFAGFGSPCVLAI 261
           F+P     +NF++++W+Y+++CF+YKW L    +L +R+ +FE++WA+  G+G       
Sbjct: 206 FIPYT-NFINFVIITWLYSFWCFDYKWILRGKWNLLQRIQYFETHWAYMFGYGLIFTTCS 265

Query: 262 FFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYAANTLSMKL 321
           FFF  L+  A+ +IL+PLF++ +  +   + +       K     ++PIFY    +   +
Sbjct: 266 FFFPMLIGNAIFSILYPLFIILSISAKPTKMVNQDGILPK-----QIPIFYVPEIIVNVI 283

Query: 322 LTLF 325
           L L+
Sbjct: 326 LKLY 283

BLAST of CSPI05G21220 vs. ExPASy Swiss-Prot
Match: Q08DE5 (Etoposide-induced protein 2.4 homolog OS=Bos taurus OX=9913 GN=EI24 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.3e-15
Identity = 72/242 (29.75%), Postives = 114/242 (47.11%), Query Frame = 0

Query: 45  KLLIRTGQCFLLNGLIFLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSF 104
           +++ R  QC   NG +F  SLL+   V IP LQ +      +I    L+       ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TAQIIGDPSLHG-----DVWSW 125

Query: 105 LRLGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNT 164
           L   L  +F   W  P+++ S +++ IW+ DIA   F      E +   P          
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAF------EVSGRKPHP-------- 185

Query: 165 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--LVGKTLNFLLLSWMYAYY 224
                      V   I + L++LLL   F L+       P  LVG+ ++ L +S +Y+ Y
Sbjct: 186 --------FPSVSKIIADMLFNLLLQALFLLQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 225 CFEYKWNLSDVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYAVMAILFPLFVL 284
           CFEY+W    + + +RL   E NW ++ GFG P   L     S +VS  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIVSGCLFSILFPLFII 277

BLAST of CSPI05G21220 vs. ExPASy Swiss-Prot
Match: Q4KM77 (Etoposide-induced protein 2.4 homolog OS=Rattus norvegicus OX=10116 GN=Ei24 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-14
Identity = 71/242 (29.34%), Postives = 114/242 (47.11%), Query Frame = 0

Query: 45  KLLIRTGQCFLLNGLIFLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSF 104
           +++ R  QC   NG +F  SLL+   V IP LQ +      VI    L+       ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TARVIGDPSLHG-----DVWSW 125

Query: 105 LRLGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNT 164
           L   L  +F   W  P+++ S +++ IW+ DIA   F      E +   P          
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAF------EVSGRKPHP-------- 185

Query: 165 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--LVGKTLNFLLLSWMYAYY 224
                      V   I + L++LLL   F ++       P  LVG+ ++ L +S +Y+ Y
Sbjct: 186 --------FPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 225 CFEYKWNLSDVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYAVMAILFPLFVL 284
           CFEY+W    + + +RL   E NW ++ GFG P   L     S ++S  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFII 277

BLAST of CSPI05G21220 vs. ExPASy Swiss-Prot
Match: O14681 (Etoposide-induced protein 2.4 homolog OS=Homo sapiens OX=9606 GN=EI24 PE=1 SV=4)

HSP 1 Score: 81.6 bits (200), Expect = 1.8e-14
Identity = 70/242 (28.93%), Postives = 114/242 (47.11%), Query Frame = 0

Query: 45  KLLIRTGQCFLLNGLIFLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSF 104
           +++ R  QC   NG +F  SLL+   V IP LQ +      +I    L+       ++S+
Sbjct: 66  RIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSV---TARIIGDPSLHG-----DVWSW 125

Query: 105 LRLGLIQLFYLFWFYPMYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNT 164
           L   L  +F   W  P+++ S +++ IW+ DIA   F      E +   P          
Sbjct: 126 LEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAF------EVSGRKPHP-------- 185

Query: 165 AQTGKSIGLGRVVIGIGEQLYSLLLINSFFLEVYATGFLP--LVGKTLNFLLLSWMYAYY 224
                      V   I + L++LLL   F ++       P  LVG+ ++ L +S +Y+ Y
Sbjct: 186 --------FPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLY 245

Query: 225 CFEYKWNLSDVSLDRRLDFFESNWAFFAGFGSP-CVLAIFFFSPLVSYAVMAILFPLFVL 284
           CFEY+W    + + +RL   E NW ++ GFG P   L     S ++S  + +ILFPLF++
Sbjct: 246 CFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFII 277

BLAST of CSPI05G21220 vs. ExPASy TrEMBL
Match: A0A0A0KPW5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587090 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.7e-186
Identity = 337/338 (99.70%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 42  MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 101

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP
Sbjct: 102 FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 161

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI
Sbjct: 162 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 221

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLP VGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD
Sbjct: 222 GEQLYSLLLINSFFLEVYATGFLPSVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 281

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK
Sbjct: 282 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 341

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL
Sbjct: 342 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 379

BLAST of CSPI05G21220 vs. ExPASy TrEMBL
Match: A0A1S3BE01 (protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.2e-179
Identity = 326/338 (96.45%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDS A  LI+VVGEK RRASQLWLEGFIDACCLHR+IILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDS-AEPLIEVVGEKLRRASQLWLEGFIDACCLHRIIILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLL+LNSVIIPTLQWILPDQQSVINSHKL+AFGATLKLYSFLRLGLIQLFYLFWFYP
Sbjct: 61  FLGSLLMLNSVIIPTLQWILPDQQSVINSHKLSAFGATLKLYSFLRLGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAM QSEPTAANPS QNEGSFNTAQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMGQSEPTAANPSRQNEGSFNTAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAEQDIYSQRATWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRATWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQ+KDL
Sbjct: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKDL 337

BLAST of CSPI05G21220 vs. ExPASy TrEMBL
Match: A0A6J1H083 (protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 307/338 (90.83%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDS A  LIK +GEK+RRASQLWLEGFIDACCLHRV+ILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDS-AEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSV+IPTLQWILPDQQS+I SHKL AFG  LKLYSFLR GLIQLFYLFWFYP
Sbjct: 61  FLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAM QSEPTA + S  N+GS N AQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLP VGKTLNF+LLSWMYAYYCFEYKWNLS+VSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAE+DIYSQR TWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQ+K+L
Sbjct: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CSPI05G21220 vs. ExPASy TrEMBL
Match: A0A6J1K7C5 (protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.7e-167
Identity = 302/336 (89.88%), Postives = 315/336 (93.75%), Query Frame = 0

Query: 3   SAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFL 62
           ++A  LIK  GEK+RRASQLWLEGFIDACCLHRV+ILCRRSKKLLIRTGQCFLLNGLIFL
Sbjct: 2   NSAEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFL 61

Query: 63  GSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMY 122
           GSLLILNSV+IPTLQWILPDQQS+I SHKL AFG  LKLYSFLR GLIQLFYLFWFYPMY
Sbjct: 62  GSLLILNSVVIPTLQWILPDQQSMIGSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYPMY 121

Query: 123 IFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGIGE 182
           IFSFILSNIWYNDIAKHGFAAM QS PT  +PS  N+GS N AQTGKS GLGRVVIGIGE
Sbjct: 122 IFSFILSNIWYNDIAKHGFAAMGQSGPTTVDPSGLNDGSSNAAQTGKSNGLGRVVIGIGE 181

Query: 183 QLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFF 242
           QLYSLLLINSFFLEVYATGFLP VGKTLNF+LLSWMYAYYCFEYKWNLS+VSLDRRLDFF
Sbjct: 182 QLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLDFF 241

Query: 243 ESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCA 302
           ESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAE+DIYSQR TWKCA
Sbjct: 242 ESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCA 301

Query: 303 GLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           GLGRLPIFYAANTLSMKLLTLFPHESQEKMQQ+K+L
Sbjct: 302 GLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CSPI05G21220 vs. ExPASy TrEMBL
Match: A0A6J1H1Y7 (protein EI24 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.4e-157
Identity = 288/317 (90.85%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDS A  LIK +GEK+RRASQLWLEGFIDACCLHRV+ILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDS-AEPLIKDLGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSV+IPTLQWILPDQQS+I SHKL AFG  LKLYSFLR GLIQLFYLFWFYP
Sbjct: 61  FLGSLLILNSVVIPTLQWILPDQQSMIGSHKLAAFGGALKLYSFLRHGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAM QSEPTA + S  N+GS N AQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMGQSEPTAVDSSGLNDGSSNAAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLP VGKTLNF+LLSWMYAYYCFEYKWNLS+VSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAE+DIYSQR TWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWK 300

Query: 301 CAGLGRLPIFYAANTLS 318
           CAGLGRLPIFYAANTLS
Sbjct: 301 CAGLGRLPIFYAANTLS 316

BLAST of CSPI05G21220 vs. NCBI nr
Match: XP_004135349.3 (protein EI24 homolog isoform X1 [Cucumis sativus] >KAE8648657.1 hypothetical protein Csa_008091 [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 1.1e-186
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP
Sbjct: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL
Sbjct: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 338

BLAST of CSPI05G21220 vs. NCBI nr
Match: XP_031740896.1 (protein EI24 homolog isoform X2 [Cucumis sativus])

HSP 1 Score: 638.3 bits (1645), Expect = 3.9e-179
Identity = 330/338 (97.63%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP
Sbjct: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSW+       YKWNLSDVSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWI-------YKWNLSDVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL
Sbjct: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 331

BLAST of CSPI05G21220 vs. NCBI nr
Match: XP_008445992.1 (PREDICTED: protein EI24 homolog [Cucumis melo])

HSP 1 Score: 637.1 bits (1642), Expect = 8.6e-179
Identity = 326/338 (96.45%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDS A  LI+VVGEK RRASQLWLEGFIDACCLHR+IILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDS-AEPLIEVVGEKLRRASQLWLEGFIDACCLHRIIILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLL+LNSVIIPTLQWILPDQQSVINSHKL+AFGATLKLYSFLRLGLIQLFYLFWFYP
Sbjct: 61  FLGSLLMLNSVIIPTLQWILPDQQSVINSHKLSAFGATLKLYSFLRLGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAM QSEPTAANPS QNEGSFNTAQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMGQSEPTAANPSRQNEGSFNTAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAEQDIYSQRATWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRATWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQ+KDL
Sbjct: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKDL 337

BLAST of CSPI05G21220 vs. NCBI nr
Match: XP_038877853.1 (protein EI24 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 603.6 bits (1555), Expect = 1.1e-168
Identity = 307/335 (91.64%), Postives = 316/335 (94.33%), Query Frame = 0

Query: 4   AAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLG 63
           AA  LIKVVGEK RRASQLWLEGFIDACCLHRV+ILCRRSKKLLIRTGQCFLLNGLIFLG
Sbjct: 3   AAEPLIKVVGEKFRRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLIFLG 62

Query: 64  SLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYI 123
           SLLILNSVIIPTLQWILPDQQSVI+SH L AFG  LKLYSFLRLGLIQLFYLFWFYPMYI
Sbjct: 63  SLLILNSVIIPTLQWILPDQQSVIDSHNLGAFGGILKLYSFLRLGLIQLFYLFWFYPMYI 122

Query: 124 FSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGIGEQ 183
           FSFILSNIWYNDIAKHGFAAM QSEP   + S +N+GS N AQTG+S GLGRVVIGIGEQ
Sbjct: 123 FSFILSNIWYNDIAKHGFAAMGQSEPAVVDLSRRNDGSSNAAQTGRSFGLGRVVIGIGEQ 182

Query: 184 LYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFFE 243
           LYSLLLIN+FFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLS+VSLDRRLDFFE
Sbjct: 183 LYSLLLINTFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSEVSLDRRLDFFE 242

Query: 244 SNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAG 303
           SNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAEQDIYSQR TWKCAG
Sbjct: 243 SNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRTTWKCAG 302

Query: 304 LGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           LGRLPIFYAANTLSMKLLTLFP ESQEKMQQ+KDL
Sbjct: 303 LGRLPIFYAANTLSMKLLTLFPRESQEKMQQNKDL 337

BLAST of CSPI05G21220 vs. NCBI nr
Match: XP_023515037.1 (protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-168
Identity = 307/338 (90.83%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MDSAAHALIKVVGEKSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLI 60
           MDS A  LIK  GEK+RRASQLWLEGFIDACCLHRV+ILCRRSKKLLIRTGQCFLLNGLI
Sbjct: 1   MDS-AEPLIKDFGEKARRASQLWLEGFIDACCLHRVVILCRRSKKLLIRTGQCFLLNGLI 60

Query: 61  FLGSLLILNSVIIPTLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYP 120
           FLGSLLILNSV+IPTLQWILPDQQS+INSHKL AFG  LKLYSFLR GLIQLFYLFWFYP
Sbjct: 61  FLGSLLILNSVVIPTLQWILPDQQSMINSHKLGAFGGALKLYSFLRHGLIQLFYLFWFYP 120

Query: 121 MYIFSFILSNIWYNDIAKHGFAAMEQSEPTAANPSTQNEGSFNTAQTGKSIGLGRVVIGI 180
           MYIFSFILSNIWYNDIAKHGFAAM QS PTA +PS  N+GS N AQTGKSIGLGRVVIGI
Sbjct: 121 MYIFSFILSNIWYNDIAKHGFAAMGQSGPTAVDPSGLNDGSSNAAQTGKSIGLGRVVIGI 180

Query: 181 GEQLYSLLLINSFFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLD 240
           GEQLYSLLLINSFFLEVYATGFLP VGKTLNF+LLSWMYAYYCFEYKWNLS+VSLDRRLD
Sbjct: 181 GEQLYSLLLINSFFLEVYATGFLPFVGKTLNFMLLSWMYAYYCFEYKWNLSEVSLDRRLD 240

Query: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWK 300
           FFESNWAFFAGFGSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAE+DIYSQR TWK
Sbjct: 241 FFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWK 300

Query: 301 CAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQSKDL 339
           CAGLGR PIFYAANTLSMKLLTLFPHESQEKMQQ+K+L
Sbjct: 301 CAGLGRFPIFYAANTLSMKLLTLFPHESQEKMQQNKEL 337

BLAST of CSPI05G21220 vs. TAIR 10
Match: AT4G06676.1 (CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 Blast hits to 262 proteins in 100 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 79; Viruses - 0; Other Eukaryotes - 39 (source: NCBI BLink). )

HSP 1 Score: 401.4 bits (1030), Expect = 7.4e-112
Identity = 210/320 (65.62%), Postives = 249/320 (77.81%), Query Frame = 0

Query: 15  KSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVIIP 74
           KS++   LWLEGF +AC LHRV+ILC RS+KLL+RTGQCFLLNGLIFLGSL +    I P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 75  TLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYIFSFILSNIWYN 134
           +LQWILPDQ S + S +  ++G     Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 135 DIAKHGFAAMEQSEPTAANPSTQNE--GSFNTAQTGKSIGLGRVVIGIGEQLYSLLLINS 194
           DIAKHGF A+E S+  +A    Q E   S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 195 FFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFFESNWAFFAGF 254
           FFLEV   G +P +GK LNFLLLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 255 GSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYA 314
           GSPCVLAIFF SPLVS A+MAILFPLFVLTATGS  E+ I + R TWKCAGLG+LPIFY 
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 303

Query: 315 ANTLSMKLLTLF----PHES 329
           A+TLSM  L++F    PHE+
Sbjct: 304 ADTLSMLALSIFRLESPHEN 320

BLAST of CSPI05G21220 vs. TAIR 10
Match: AT4G06676.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 261 Blast hits to 259 proteins in 97 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 81; Viruses - 0; Other Eukaryotes - 34 (source: NCBI BLink). )

HSP 1 Score: 339.7 bits (870), Expect = 2.6e-93
Identity = 177/268 (66.04%), Postives = 209/268 (77.99%), Query Frame = 0

Query: 15  KSRRASQLWLEGFIDACCLHRVIILCRRSKKLLIRTGQCFLLNGLIFLGSLLILNSVIIP 74
           KS++   LWLEGF +AC LHRV+ILC RS+KLL+RTGQCFLLNGLIFLGSL +    I P
Sbjct: 4   KSKQVLLLWLEGFREACSLHRVVILCLRSRKLLLRTGQCFLLNGLIFLGSLGVFKWFIDP 63

Query: 75  TLQWILPDQQSVINSHKLNAFGATLKLYSFLRLGLIQLFYLFWFYPMYIFSFILSNIWYN 134
           +LQWILPDQ S + S +  ++G     Y+FLR GL+QLFY+FWFYP+Y+ SFILSNIWYN
Sbjct: 64  SLQWILPDQCSPLTSQEFCSYGG---FYAFLRGGLLQLFYVFWFYPLYMLSFILSNIWYN 123

Query: 135 DIAKHGFAAMEQSEPTAANPSTQNE--GSFNTAQTGKSIGLGRVVIGIGEQLYSLLLINS 194
           DIAKHGF A+E S+  +A    Q E   S N A   +  GLG V+IGIGEQ+YS+LL+  
Sbjct: 124 DIAKHGFEAIEISDLNSAEALRQGEALASLNMANAERPSGLGGVMIGIGEQVYSILLLTF 183

Query: 195 FFLEVYATGFLPLVGKTLNFLLLSWMYAYYCFEYKWNLSDVSLDRRLDFFESNWAFFAGF 254
           FFLEV   G +P +GK LNFLLLSWMYAYYC+EYKWN S +SL +RLDFF+SNWAFFAGF
Sbjct: 184 FFLEVCVVGVIPYIGKILNFLLLSWMYAYYCYEYKWNFSGISLKKRLDFFQSNWAFFAGF 243

Query: 255 GSPCVLAIFFFSPLVSYAVMAILFPLFV 281
           GSPCVLAIFF SPLVS A+MAILFPL V
Sbjct: 244 GSPCVLAIFFLSPLVSGALMAILFPLNV 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5BPL51.0e-11065.63Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1[more]
Q54PW93.4e-2127.63Protein EI24 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284253 PE=3 S... [more]
Q08DE56.3e-1529.75Etoposide-induced protein 2.4 homolog OS=Bos taurus OX=9913 GN=EI24 PE=2 SV=1[more]
Q4KM771.4e-1429.34Etoposide-induced protein 2.4 homolog OS=Rattus norvegicus OX=10116 GN=Ei24 PE=1... [more]
O146811.8e-1428.93Etoposide-induced protein 2.4 homolog OS=Homo sapiens OX=9606 GN=EI24 PE=1 SV=4[more]
Match NameE-valueIdentityDescription
A0A0A0KPW52.7e-18699.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587090 PE=4 SV=1[more]
A0A1S3BE014.2e-17996.45protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1[more]
A0A6J1H0831.9e-16890.83protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE... [more]
A0A6J1K7C53.7e-16789.88protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4... [more]
A0A6J1H1Y73.4e-15790.85protein EI24 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE... [more]
Match NameE-valueIdentityDescription
XP_004135349.31.1e-186100.00protein EI24 homolog isoform X1 [Cucumis sativus] >KAE8648657.1 hypothetical pro... [more]
XP_031740896.13.9e-17997.63protein EI24 homolog isoform X2 [Cucumis sativus][more]
XP_008445992.18.6e-17996.45PREDICTED: protein EI24 homolog [Cucumis melo][more]
XP_038877853.11.1e-16891.64protein EI24 homolog isoform X1 [Benincasa hispida][more]
XP_023515037.11.8e-16890.83protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 h... [more]
Match NameE-valueIdentityDescription
AT4G06676.17.4e-11265.63CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 ... [more]
AT4G06676.22.6e-9366.04FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF07264EI24coord: 32..277
e-value: 3.4E-38
score: 131.7
IPR009890Etoposide-induced 2.4PANTHERPTHR21389P53 INDUCED PROTEINcoord: 5..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G21220.1CSPI05G21220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane