CSPI05G20310 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G20310
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	GRAM domain-containing protein
Location	Chr5: 21431771 .. 21432736 (-)
RNA-Seq Expression	CSPI05G20310
Synteny	CSPI05G20310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTATTTTGGTTCGAAAAAAAATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATGTAAGAGTGAAGAAAAAAAAAATATTTCATTTTGGTTGAATATATATAAAATAGTTTGCTAACTAAATTTGGTTTTTGAGTTACTTGTAGATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGGTAAGTCTTTTTTTATAGATATCATCGATCTGGTACATTATGCAAAGTGTAATTAATTTTGATGATTTGAGGTTGAATTTTGCAGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTAAGTCTCTAATTTATATATACATACACTAAAATAACTGATCAGTTTTTAATTTGGGTAATTAAAATTTTCATTAGTACTGATGAAGGTTAATTAATGTTTGCATTACAGGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG

mRNA sequence

CTTTTATTTTGGTTCGAAAAAAAATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG

Coding sequence (CDS)

ATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG

Protein sequence

MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW*

Homology

[Close]

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.6e-51
Identity = 109/216 (50.46%), Postives = 154/216 (71.30%), Query Frame = 0


Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHL-SSSDDGFQSSIKKYNGKDCILDRLNKNG 64
           +++ ++ +P + A +   YL  P+  N+  + +SS    QS++K       IL R     
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS------ILKR----- 65

Query: 65  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 124
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ 
Sbjct: 66  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 125

Query: 125 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 184
           QCYLSTTAGP+ GLLFIS+ K+AFCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+
Sbjct: 126 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 185

Query: 185 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

[Close]

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 7.8e-51
Identity = 107/215 (49.77%), Postives = 150/215 (69.77%), Query Frame = 0


Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGK 64
           + + ++ FP ++  S A YL  P+  N+L + +S    + S     GK  +        K
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTSS---KFSFLTGKGKSML------RKK 65

Query: 65  KTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQ 124
           K D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  Q
Sbjct: 66  KNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQ 125

Query: 125 CYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESEN 184
           CYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N
Sbjct: 126 CYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQN 185

Query: 185 VKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
             K S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 TTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 210

[Close]

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 5.1e-50
Identity = 94/156 (60.26%), Postives = 125/156 (80.13%), Query Frame = 0


Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 174

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L+
Sbjct: 175 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 210

[Close]

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.3e-49
Identity = 91/156 (58.33%), Postives = 123/156 (78.85%), Query Frame = 0


Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTT G + GLLFIS+ KIAFCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S+RY+++ T+DN++FWFMGF++YQ  FN L+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

[Close]

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 71/183 (38.80%), Postives = 115/183 (62.84%), Query Frame = 0


Query: 42  SSIKKYNGKDCILDRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGAR 101
           +S      KD +L+ LN+ GKK ++       ++  L++H+K    IS+    +LS G +
Sbjct: 38  TSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTK 97

Query: 102 ILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKI- 161
           ++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +IAFCSD +I++ 
Sbjct: 98  MIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLP 157

Query: 162 ASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTF 217
           +S  G+ +  +YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   F
Sbjct: 158 SSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAF 217

[Close]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL
Match: A0A0A0KPM2 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579030 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 6.5e-109
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 73
           MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCIL RLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILKRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQW 219
           ETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQW 205

[Close]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL
Match: A0A1S3BEG1 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.7e-107
Identity = 201/218 (92.20%), Postives = 207/218 (94.95%), Query Frame = 0


Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHMLGF MSLALSRARYL HP  RLHLSSSDDG QSSIKKYNGKDCIL+RLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GK+TDNIIHALREHVKLG KISE VKGKLSLGA+ILRVGG+RKIYKKLFSM+EEEKLLKV
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASPNGDHIRI YKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRILYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 219
           ENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQW 218

[Close]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL
Match: A0A5A7SSL2 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00700 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.4e-103
Identity = 202/218 (92.66%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 3   TTIQEHMLGFPMSLALSRARYLSH-PSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKN 62
           TTIQEHMLGF MS ALSRARYL H PS  LH+SSSDD   QSSIKKYNGKDCIL+RLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 221

[Close]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL
Match: A0A1S3CNX8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.4e-103
Identity = 202/218 (92.66%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 3   TTIQEHMLGFPMSLALSRARYLSH-PSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKN 62
           TTIQEHMLGF MS ALSRARYL H PS  LH+SSSDD   QSSIKKYNGKDCIL+RLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 221

[Close]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL
Match: A0A0A0KT32 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579000 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 195/205 (95.12%), Postives = 196/205 (95.61%), Query Frame = 0


Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKNGKKTDNIIHALR 73
           MSLALSRAR L H S  LH+SSSDD   QSSIKKYNGKDCIL RLNKNGKKTDNIIHALR
Sbjct: 1   MSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNKNGKKTDNIIHALR 60

Query: 74  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 133
           EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL
Sbjct: 61  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 120

Query: 134 PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 193
           PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ
Sbjct: 121 PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 180

Query: 194 IETLDNFEFWFMGFLNYQSTFNSLQ 218
           IETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 IETLDNFEFWFMGFLNYQSTFNSLQ 205

[Close]

BLAST of CSPI05G20310 vs. NCBI nr
Match: XP_011655497.2 (putative GEM-like protein 8 [Cucumis sativus])

HSP 1 Score: 433.0 bits (1112), Expect = 1.6e-117
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN
Sbjct: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV
Sbjct: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 219
           ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 218

[Close]

BLAST of CSPI05G20310 vs. NCBI nr
Match: KGN52180.2 (hypothetical protein Csa_007871 [Cucumis sativus])

HSP 1 Score: 406.0 bits (1042), Expect = 2.1e-109
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 73
           MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQW 219
           ETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQW 205

[Close]

BLAST of CSPI05G20310 vs. NCBI nr
Match: XP_011655499.1 (GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572 [Cucumis sativus])

HSP 1 Score: 400.2 bits (1027), Expect = 1.1e-107
Identity = 207/218 (94.95%), Postives = 208/218 (95.41%), Query Frame = 0


Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNK 60
           MKTTIQEHMLGF MSLALSRAR L H S  LH+SSSDD   QSSIKKYNGKDCIL RLNK
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180
           VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180

Query: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218

[Close]

BLAST of CSPI05G20310 vs. NCBI nr
Match: XP_008445894.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 397.9 bits (1021), Expect = 5.7e-107
Identity = 201/218 (92.20%), Postives = 207/218 (94.95%), Query Frame = 0


Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHMLGF MSLALSRARYL HP  RLHLSSSDDG QSSIKKYNGKDCIL+RLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GK+TDNIIHALREHVKLG KISE VKGKLSLGA+ILRVGG+RKIYKKLFSM+EEEKLLKV
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASPNGDHIRI YKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRILYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 219
           ENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQW 218

[Close]

BLAST of CSPI05G20310 vs. NCBI nr
Match: XP_008465496.1 (PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 385.6 bits (989), Expect = 2.9e-103
Identity = 202/218 (92.66%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 3   TTIQEHMLGFPMSLALSRARYLSH-PSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKN 62
           TTIQEHMLGF MS ALSRARYL H PS  LH+SSSDD   QSSIKKYNGKDCIL+RLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 221

[Close]

BLAST of CSPI05G20310 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 202.6 bits (514), Expect = 3.3e-52
Identity = 109/216 (50.46%), Postives = 154/216 (71.30%), Query Frame = 0


Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHL-SSSDDGFQSSIKKYNGKDCILDRLNKNG 64
           +++ ++ +P + A +   YL  P+  N+  + +SS    QS++K       IL R     
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS------ILKR----- 65

Query: 65  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 124
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ 
Sbjct: 66  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 125

Query: 125 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 184
           QCYLSTTAGP+ GLLFIS+ K+AFCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+
Sbjct: 126 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 185

Query: 185 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

[Close]

BLAST of CSPI05G20310 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 201.8 bits (512), Expect = 5.5e-52
Identity = 107/215 (49.77%), Postives = 150/215 (69.77%), Query Frame = 0


Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGK 64
           + + ++ FP ++  S A YL  P+  N+L + +S    + S     GK  +        K
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTSS---KFSFLTGKGKSML------RKK 65

Query: 65  KTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQ 124
           K D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  Q
Sbjct: 66  KNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQ 125

Query: 125 CYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESEN 184
           CYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N
Sbjct: 126 CYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQN 185

Query: 185 VKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
             K S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 TTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 210

[Close]

BLAST of CSPI05G20310 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-51
Identity = 94/156 (60.26%), Postives = 125/156 (80.13%), Query Frame = 0


Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 236

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L+
Sbjct: 237 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 272

[Close]

BLAST of CSPI05G20310 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 195.3 bits (495), Expect = 5.2e-50
Identity = 91/156 (58.33%), Postives = 123/156 (78.85%), Query Frame = 0


Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTT G + GLLFIS+ KIAFCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S+RY+++ T+DN++FWFMGF++YQ  FN L+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

[Close]

BLAST of CSPI05G20310 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-33
Identity = 71/183 (38.80%), Postives = 115/183 (62.84%), Query Frame = 0


Query: 42  SSIKKYNGKDCILDRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGAR 101
           +S      KD +L+ LN+ GKK ++       ++  L++H+K    IS+    +LS G +
Sbjct: 38  TSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTK 97

Query: 102 ILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKI- 161
           ++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +IAFCSD +I++ 
Sbjct: 98  MIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLP 157

Query: 162 ASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTF 217
           +S  G+ +  +YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   F
Sbjct: 158 SSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAF 217

The following BLAST results are available for this feature:

BLAST of CSPI05G20310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FTA0	4.6e-51	50.46	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW4	7.8e-51	49.77	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9FMW6	5.1e-50	60.26	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW5	7.3e-49	58.33	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9M122	2.1e-32	38.80	GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1	[more]

BLAST of CSPI05G20310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPM2	6.5e-109	99.51	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579030 PE=3 S...	[more]
A0A1S3BEG1	2.7e-107	92.20	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1	[more]
A0A5A7SSL2	1.4e-103	92.66	GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3CNX8	1.4e-103	92.66	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1	[more]
A0A0A0KT32	1.1e-100	95.12	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579000 PE=3 S...	[more]

BLAST of CSPI05G20310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655497.2	1.6e-117	100.00	putative GEM-like protein 8 [Cucumis sativus]	[more]
KGN52180.2	2.1e-109	100.00	hypothetical protein Csa_007871 [Cucumis sativus]	[more]
XP_011655499.1	1.1e-107	94.95	GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572...	[more]
XP_008445894.1	5.7e-107	92.20	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]
XP_008465496.1	2.9e-103	92.66	PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [C...	[more]

BLAST of CSPI05G20310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08350.1	3.3e-52	50.46	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	5.5e-52	49.77	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	3.6e-51	60.26	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	5.2e-50	58.33	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT4G01600.1	1.5e-33	38.80	GRAM domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 103..181 e-value: 6.4E-18 score: 75.5
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 106..216 e-value: 3.5E-19 score: 68.8
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 101..218 e-value: 1.9E-20 score: 74.7
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 1..217
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 1..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G20310.1	CSPI05G20310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity