CSPI05G17740 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G17740
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDolichyl-phosphate beta-glucosyltransferase
LocationChr5: 18930247 .. 18937666 (-)
RNA-Seq ExpressionCSPI05G17740
SyntenyCSPI05G17740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTATCCATGGACGATACTGTTCATTTTCATTAGCCTCGCTCTTTGTTTTCCTGCAGCTTTGATATCTACTCTCATCATAGAAGCTTACAAGAGGAGATTCAATCGCGAGTACGCTACTGCAGCATTCATCGTTGATTTCAAATTCATTTTCATTTCTCATATTCTCATATATGATCTGTCTTTATTAGACCCTCTTTGTATTCATTCGTTTTGGTTTGTTTCAGGCACGTTGGAGCTCCAGCAATCTTCGAGGACCCAAACTCGTTGAAACGGGTAACTTCTTTTTTTTTTTTTTTACCTCATCGTCTGAAAGATTGAATCATACGTTTAATATCGACTCTCAATCACTCCAAGATTGTCTTTAAAGAAGTTTAATTTATAGGTAGCTAGAGCTTTAGTCGAACGATATAGAATTTGATCGTGGGATTGTATGTACCTTCATAGCTTTTACAAAGTGTTGCTTTCAATTTGCACATAATAGATGTGAGGATAGGTTGCAGATCTTGTTTTTTTTCTGTAAAATTTCGAGCATTGCGCTCAAACCATATAGATTGCAAAAAAGCCCGAATGGAGTTTAGACTGTGTTTTCCTTTCCTTCTATGATGGATGAACACAAGAGAAAGATAGTCCTCAGTATTGTTAGGAAAGACAGTCTGCCAACCAAATGCTTCTAAAATGTAACCCCATCTCTTCTTATTATCCCAAACCATACATGGGGATGTTCTCTTAGAATTAGTGGGTTTGGCCCACGCAAAGGATCTACTCTAATATTCATTTATTTTTTATCATTAGCTGTAACCTATTTATCGTCCCTCTATTATCTTTTGTTAATATGAAAAATTAATAAGAAAGTTGATCGTGGTTTTTCTCCCGGTACTTGAGTTTGAACACAACTCGGTGTCTACTTTACTTTACCGATTTTAATATGGTATCAAAGCGAGGTAAAGACGAAACACTAGACACAGGTATAGATGAAACCCAAACAGAGACAGCCGCTGCCACTGTTGTCGTTGTGGATGCCGATGTGGAAAAGCTGCTCCACCAGGTTTAGAAGACGCTGGCAGTCACTGTAGGCCTACCCTTGGAGTCAGCTGCGCAGCCCAGTGGCGAAAACCAGCCGAATGTGCTTCAATTAACCGCCCTGCGCTCGCCGCTGCTCAGCCGGTCTACCCCAATCTGTCTTCCCAAGTCCAGCTGCAATACCCTTTTGGTCAGCCACACCTCCACGCACCGCCGCCGCCCTCGGTTTATGGACTGCCATCGGCCTTTGATTCTTTCAGCAGTCGCACGCCCATGGTACCATCTAAAGGTGCCTTTCCAAGAGAGAAGCTGAATGACTAGAATTACTTTTCTTGGTCTTAATCGACCATGATGTTTCTTGAGGGTCCTCACTAATTTGGTTTTCTGACCAGGGAGACTCTTCGTCCCCCACCTGGTACTGTCCTAGAACCTTTTTGTAAAGGGAGGACTCCTTTATACGGTCCATGTTGATTAATAGTATGTAACCACAAATAGGCAAGCATTTGCTTTATGCAACAGTTACGAAGGATCTATGGGACACAACTCAAAAACTCTATTCAAAGTGTCAGAACGCTTCTCCTTTGTATATACTGAGGAAACAAGTCCATGATTGCAAGCAAGGAACTCTGGATGTAACCTCCTACTTCAATGAACTTTCTCTACTTTGGCAAGAGATGGACCTCCGCAACAAGATAGTATGGGATACTCCGATACAATATGCTAGACTTGAAAAAGTTGATCGGATTTATGACTTCCTTGCAGGTCTCAACCCCAAGTTTGACATATTTTGTGGTCATATACTGACTCTGTTTCTACTTTGGAACAAGATGAGTTTGGTAGCTTTTGGGGTTAAAATCTGTCTTTCCCTCCAGTAATCAGTGTTGCAGTTGGTCTATTTCAATTACGTTGTCCCACTGTATCTGTAAGTCCTTTCCAAAGCCCTTCTGAAGTCTATTGAAGACTTTTGTTTCAACTTTAAAGTATGTGACTTGTACACTCAATCAAGGACAGCCTGAGGCTACTTCAGCAGTTAACGGCGGGCAATATTTGGTTCCTAGGATTATGTCTCAGATTTCTGCCACAGGATGTTCTGTTGTTGATTGTAGTTACTCCGTGATTTAATGAGTAACGCTTGAGAAATTGGATCTAACAACCTTGTTGAAGAATGAGACCTTTAGGTTAAATACTAAAAAACTACAAATCAATATGATCTCAACTTAGGTTTGATTTCAGATTTCTTAGATGGTCAGCGAAACCAGGGATTGGACAAAGCTTGTGGCTCCATCAATCCTTGATCTTCAGTTCACTCCACAACATAGCTTGATCAAGACTACTTAAATGATCCTAGCATGCAAATCTGAACATAAGACATGCAAAGTAAGTTGCTCATCGCCGTAGGCTCAAAAGACTAATTTCATTGAAATTTGACAAAATTTGATCTCCAATTTGGGTTACAATAAAGATGACTTATATGACCTTCAAAACACAACCTAAATCCTTCCACTAAGTCGTTTTACTGTTTGTAAGCTCCCAAACGTTAATGGTACAAAGGGCCACCAACAAAAGTAAACCTAATACAATACATTAAAGGTCTTTAAACTGAAAAGAAACTAATAAAGCTAAAAAATAAAAAAAAATTGATCATTTTCCCTCAGCCACCCAAGAGTATGTAATCATCCAAGAATAATTGAAACTTCAGTTGAAGTAGTTTAGGTTTAGTGTAGGTTGTGTAGCTTGAAATCTAGTTCCTTGAATTAAGCTTGAATCGTCTTAAATTAAGTTTGAACGCACTAAATGGAACTCATTCTTGAAGTTATTATGGTGTGATGCCTTTATTCGTATCAATGAGATTTGAGTAATCTGATCTTGCTTGATTGAGAAACTGATTTGCATTTTGTAGGCCTTTGCAAGGCTCTAGTACTCAGGATAACAAGGGAAGGAAAACTTTCTTGAATGCTTTTTGTTGAATTATTCTGATAATGAGTACGGTTATATAAAGGCAGAACTCTATTACCTTTTACTATTACTTAGAAAGTTAGAACTCAATTAGATAATAATAAAATTAATAATTACTGCTAATTAACAACCACTGCAAGCACCTAATATCTCTTTGCACCCGGCAAGCCAGTAGTTGGTGCACACAAAATATTGAATTTGAGCTTGTTTTTTAATAGGTGAAAATTCAAAGAGAGTATACTGTAGAGAGAGGTTTGGTTAGGATGTTGTAATCTGAGTTGAGCTGGAAGAGGTTGAACACTGTAGTTCTTGCCAAGAAGTAACTCATGTACAAAGTAAATGTCAATCTCAGCATATGTAGTGGAATATCGTGAAATAGGATTGAAGCTAAAACTTTTATTTTATTTGCATCAAGTAGTCCACAATTAGGAAATACACACAACAGAGAATCCAAAGAAAATCCCTAATGATGAAATTAATAACCAAATGGAATAATGATGACTATAATAATAATGATGAAATAAAATTTTAAGAAAAGGGGGCTCGTGGAAATGAGCTACACAGACTCCAAAATTGATGGAAACGATAAATTACATCATGCTTTTGTTTGTAATATTGGATCAATATTGATGCCAAATGTAGAATGTTTGCTAGCAAGTTCTTGAATCTTTTTCAGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCAGGGAGCTCTGGAGGAAACCATCAAGTAAGTTGAAACATCACATAAAACATGTAATTTTTTCCATATATATATTTTTACTTTTCTTTTATACCTTTTAGCTCTCTTTGACTTTGGCTTCTCTGCTTACCAATCGTCTCTACTCAGAGTTTTCCAAATTCGTAGATTTCTTAATTTTTCTCGTAATAGTCTCTACTTGGCTAGATACAACTGACAAAGTAGACCCCACGAGCATATTCCTCAACTCTCAAAGGCCACGAGCATTTTCTAATTCATGTAAAATAGTAGTAGGCCAAAAAACTTAGTAAAATCTCATTCCTCTTTTGCTTTTTAGTTATCTTCAACAACGAGCTGCGAAGGACAAGTCATTTTCCTATGAGGTATTTCTTTTCTCCATTTATTCCTTTTATTCTTGTTCCAGATCGGGACAACTCTTAGAAAATTTGTGGGTTTGTTGGGTATGAAACTTGATATGGCAATACATTCTTGCTTGCTATACCTTGATATATTCTTCTTCTTGTATTTTTCTATTTTTTATTACAAACCTGGAAATGTGTATTCAGAGAAAAAAAAAAGATAACCTATGGGGTCGGGGATGAGGAATCTCCTCCCAAAACAGCTACTTAACAAAAACACGTTCCCATCTGGAAGAATCTTGAAAATGGAGGAACAAAAGAATTTCTATAACTATCACAGTATTCAATACGAAGAATCAGGGAGCTGAACATTGTTCCAAATATAAGGAAATCATTAGGCTTATCTTCAAATGTTGTTCCATTCCCGAACCAAATACCTCATATTATCGTGACAAGCAAACTTCCACAAAAAATTTCCTCTTCATATTCAAATCACTGACTCCCTGAAGGAGCCTGCCAATTACTTGATTAGGTAGGGAAATACGAAGGCAAAACACTTCAACGAAAGCAGCCTGCATTGCCTTGCAAAGAGAAATGGACAGAAAAATGATCTAGAGTTTCCCCGTTTGTCTGTCATAGAGATCAAACGGAAGGGATACATTTTCTTTGGATTCTCTGTTGTGTGTATTGCTTCTATAGGCCACCAACCACTGGAATATCTTCACCTTCCTTGGAATTGTGTCATAGGACTTTATGCATAGGCCTAATACCCGTAAGCTCCATGGAAGGTGAATTTGTAGGAAACTCACAGGATTGGTATTAGAGGAATGTTAGGAGTACATTAGTAGTTAATTAGGGAGTTTGTTGGGGAAGTTGGTTATAAATAGAGGGGAGGGGAATGGAGAAGTTAAGCAATATTTTGGAATATGAATTAGGGCTTTAGTGTTATCTCAAGATGGGACAGATGTGAAGGGCTCAAGTACCTCGAACACTTGGTTTATCTTCTATTGCTGTTATCTTTTTATATTTCAATATATCCGGGTTCTATAAAAAAATTTACCAGATAGGTCTAAAGATCATCTTAAACTCTTCAATCCATGACCCAGTCATAAAGATTCAAGTCTTCGATTCAACTCAATCTACTTGTACCTTGATACGTTCACTTCCCTTTTCCCATTATCTCGTTAACTTGGTGAAAATAATAGCCAAACGAAAAACAAAAGACAAAGGTCAAATAAAAGTAGAGAGGATAATTGCACAAGAAATTTACAGCGTATCACTTTGAATCAGAGAGAGAATTTCATTAGATGCCCATCATACAGCTTAGAGAATGAGGATGATGGAGCGAAAGTTAAGAGAAGCTCCAAACCAGTACATGGCAATTTTACCTTCTAAATATCAGACTCTGATAAAACAAAAGAACAAAATTCCACCTTAGCATTGAGCCAAATAATCACACATTCTTTGGTCCTTTTACCTTTTAGTTTGTTTTTATTCTTTGTAAAGGAGATAAGGGTCTGAAACTTTTTTCCAAGTTTGAGTTAAATTTAAGTCTGTTTTTTCCCCAAGTACAGGTTGAGCTTTTTTATGTGTAATAGATCCTAGTTTATCGATTTTATAGTATGCTAAACTAATTAGAAACTTGTGTTTATTTTTCTTTTAAAAGTAGAAACTCATTTAAAGATATAAATGCTGATTGGTCTCTCATCTCCGTCCATCTGAGATAAAAGAATGGGAAGCTCTGGTTTCAATAATGGATGAAGCTGAATATTTAATCATCCGACTTGTATAGGTCATAATAATTGATGATGGGAGCGCGGATGGCACAAAAGGTATAGCTTTCAATTTTGTTAGGAAGCACTCTGTAGAAAATGTGAGGGTTATACTTCTCGGAAGAAATCATGGAAAAGGAGAAGCTATTAGAAAGGTGATTCTGGACTTCGCACTTTGTTTGCAACACTTGTATGGTTATTATTCTCTTTACACCATTTTATAATGGCAAATTCGTGAGATTCACGTTCTAAAAGGCATTCTCAAGCAGGGAATGCTTCACTCACGTGGTGAGATACTTCTAATGCTGGATGCCGATGGAGCAACCAAAATTACCGACCTAGAAAAACTTGAAAACCAGGTTTCAGCAAACCAATCTATTTTCCAGAGCAATTTATTTGAATGAAGTTTGCTTATGAAAGTGAAAGCCCTGACGTCTAAAATTGTGAAATAATTATTAAAGATGATTTTTTTCTCCCCCAGATTCATGCAGTAGTTAAGAGGGAAACTTCAAGAGATTCAAGCGTTAGAGTATCTGATATTCCTATCACTGCATTTGGTTCTCGTGCTCATCTTGAGGAAAATGCTTTGGCTACAGTTTAGTGTTGCTCTTGCAGTTATCTCCCTTCCTTATTCGAGTTTTATTATTTGTTTGATGGCTTCATTCACTTTTTCAGAGGAAGTGGTACCGCAATTTCTTGATGAAGGGTTTTCATCTTGTGGTTCTTCTAACTGCTGGTCCTGGAATACGGGATACACAGGTGATTATGCTAACTGTTTGCGTGCTCATGCTCCTTATATTATACGTGCCATTATCTCTGTGCTTATATTGCTGTGCAGTAATGCAGATATAATTTGGATTCATCCCAACGACTGGCTGAAGCTGCTAAGCTTCATTTTTTAATTCTGACTTCCTATAACTGTGATTATATGCAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTGTTTATTAACATTCGTTTGAAGAGGTGAGATTCTCCCCTATATTCTCTCAAGTCACGACTGCTTATTAAAGGTTTTTACTTTGGATTGTCATGTCTTATGGAGTAGATCCAGCTCTCTTTTAAGTGCATGCGACCACACCATAAAGAATATGGTAAATCGGTGTGTATTGTGGAGTGGATTACATTCAATTTGAGAGCAAAACCGTAACAAATGAACTTTGTTGGCTTATTTTGTTTCCTGTTCTCTTTCTAAATCCTTCCACTTGTTGGTAAATCATTTTCAACAGCAATAGTAAATTTCGTCCTGATCTGTGCTTCCATGATATTGCTGATTCTGATCAATGGATCATCTAAGCAACAAGTACTAACCAACTTAGGATTCACTTCCTGCAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCAGCAAATATTTTGGCATTCCTATGATTGAAGTATCCGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCTCGGAGCATTTTAAACATGCTATGGGAGCTCGCACTCATGTCGATCGGATACAAAACTGGACTCTGGAAAATTTTCAACTGA

mRNA sequence

ATGGAGTATCCATGGACGATACTGTTCATTTTCATTAGCCTCGCTCTTTGTTTTCCTGCAGCTTTGATATCTACTCTCATCATAGAAGCTTACAAGAGGAGATTCAATCGCGAGCACGTTGGAGCTCCAGCAATCTTCGAGGACCCAAACTCGTTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCAGGGAGCTCTGGAGGAAACCATCAATTATCTTCAACAACGAGCTGCGAAGGACAAGTCATTTTCCTATGAGGTCATAATAATTGATGATGGGAGCGCGGATGGCACAAAAGGTATAGCTTTCAATTTTGTTAGGAAGCACTCTGTAGAAAATGTGAGGGTTATACTTCTCGGAAGAAATCATGGAAAAGGAGAAGCTATTAGAAAGGGAATGCTTCACTCACGTGGTGAGATACTTCTAATGCTGGATGCCGATGGAGCAACCAAAATTACCGACCTAGAAAAACTTGAAAACCAGATTCATGCAGTAGTTAAGAGGGAAACTTCAAGAGATTCAAGCGTTAGAGTATCTGATATTCCTATCACTGCATTTGGTTCTCGTGCTCATCTTGAGGAAAATGCTTTGGCTACAAGGAAGTGGTACCGCAATTTCTTGATGAAGGGTTTTCATCTTGTGGTTCTTCTAACTGCTGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTGTTTATTAACATTCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCAGCAAATATTTTGGCATTCCTATGATTGAAGTATCCGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCTCGGAGCATTTTAAACATGCTATGGGAGCTCGCACTCATGTCGATCGGATACAAAACTGGACTCTGGAAAATTTTCAACTGA

Coding sequence (CDS)

ATGGAGTATCCATGGACGATACTGTTCATTTTCATTAGCCTCGCTCTTTGTTTTCCTGCAGCTTTGATATCTACTCTCATCATAGAAGCTTACAAGAGGAGATTCAATCGCGAGCACGTTGGAGCTCCAGCAATCTTCGAGGACCCAAACTCGTTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCAGGGAGCTCTGGAGGAAACCATCAATTATCTTCAACAACGAGCTGCGAAGGACAAGTCATTTTCCTATGAGGTCATAATAATTGATGATGGGAGCGCGGATGGCACAAAAGGTATAGCTTTCAATTTTGTTAGGAAGCACTCTGTAGAAAATGTGAGGGTTATACTTCTCGGAAGAAATCATGGAAAAGGAGAAGCTATTAGAAAGGGAATGCTTCACTCACGTGGTGAGATACTTCTAATGCTGGATGCCGATGGAGCAACCAAAATTACCGACCTAGAAAAACTTGAAAACCAGATTCATGCAGTAGTTAAGAGGGAAACTTCAAGAGATTCAAGCGTTAGAGTATCTGATATTCCTATCACTGCATTTGGTTCTCGTGCTCATCTTGAGGAAAATGCTTTGGCTACAAGGAAGTGGTACCGCAATTTCTTGATGAAGGGTTTTCATCTTGTGGTTCTTCTAACTGCTGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTGTTTATTAACATTCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCAGCAAATATTTTGGCATTCCTATGATTGAAGTATCCGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCTCGGAGCATTTTAAACATGCTATGGGAGCTCGCACTCATGTCGATCGGATACAAAACTGGACTCTGGAAAATTTTCAACTGA

Protein sequence

MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSIFDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGIAFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQIHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSKLSPRSILNMLWELALMSIGYKTGLWKIFN*
Homology
BLAST of CSPI05G17740 vs. ExPASy Swiss-Prot
Match: Q9VLQ1 (Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=wol PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 144/327 (44.04%), Postives = 200/327 (61.16%), Query Frame = 0

Query: 5   WTI---LFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSIF 64
           WT    L  ++   L   A  I+ L++   K   N +       F DP+++K V  PS+ 
Sbjct: 2   WTCLCQLCFYLLSTLAVAALSIAALVLYKTKPYPNIKRHKDEETFLDPHTIKTVTFPSLE 61

Query: 65  DPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGIA 124
           D     +S+IVPA+NEE RL   L+E + +L+Q++A   +F+YEVI++ DGS D T  +A
Sbjct: 62  DSPSLELSVIVPAYNEEQRLPSMLDECLAFLEQKSAGTPNFTYEVIVVSDGSQDATVSVA 121

Query: 125 FNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQI 184
             + +KH  E VRV+ L  N GKG A+R GML +RG  LL  DADGATK  D +KLE  +
Sbjct: 122 LGYSKKHGAEKVRVLELIENRGKGGAVRMGMLSARGRNLLFADADGATKFPDYDKLEVAL 181

Query: 185 HAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGPG 244
             +      RD  +        A GSRAHLE +A+ATR ++R  LM GFH +V L A   
Sbjct: 182 KQLAPE--WRDDGI--------AIGSRAHLENDAIATRSFFRTILMHGFHFLVWLFAVRS 241

Query: 245 IRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSKL 304
           IRDTQCGFK+FTR+ ARKLF ++ ++RW FDVEL+YL++   +PM EV+V W+EI GSKL
Sbjct: 242 IRDTQCGFKLFTRTTARKLFTSLHVERWAFDVELLYLAENLKLPMSEVAVRWTEIDGSKL 301

Query: 305 SP-RSILNMLWELALMSIGYKTGLWKI 328
           +P  S L M  +L ++ + Y  G W+I
Sbjct: 302 TPFWSWLQMGRDLFMIWVRYLVGAWRI 318

BLAST of CSPI05G17740 vs. ExPASy Swiss-Prot
Match: Q9Y673 (Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-66
Identity = 128/271 (47.23%), Postives = 184/271 (67.90%), Query Frame = 0

Query: 58  PSIFDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGT 117
           PSI+D   K +S++VP++NEE RL   ++E ++YL++R  +D +F+YEVI++DDGS D T
Sbjct: 57  PSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQT 116

Query: 118 KGIAFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKL 177
             +AF + +K+  + VRVI L +N GKG AIR G+  SRGE +LM DADGATK  D+EKL
Sbjct: 117 SKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKL 176

Query: 178 ENQIHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLT 237
           E  ++         D     + + I A GSRAHLE+ ++A R ++R  LM GFH +V   
Sbjct: 177 EKGLN---------DLQPWPNQMAI-ACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFL 236

Query: 238 AGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIP 297
              GIRDTQCGFK+FTR AA + F ++ ++RW FDVEL+Y++++F IP+ E++VNW+EI 
Sbjct: 237 CVKGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIE 296

Query: 298 GSKLSP-RSILNMLWELALMSIGYKTGLWKI 328
           GSKL P  S L M  +L  + + Y TG W++
Sbjct: 297 GSKLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of CSPI05G17740 vs. ExPASy Swiss-Prot
Match: Q9DB25 (Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 128/271 (47.23%), Postives = 178/271 (65.68%), Query Frame = 0

Query: 58  PSIFDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGT 117
           PSI+D   K +S++VP++NEE RL   ++E +NYL++R   D +F+YEVI++DDGS D T
Sbjct: 57  PSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALNYLEKRQKHDCTFTYEVIVVDDGSEDQT 116

Query: 118 KGIAFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKL 177
             +A  + +K+  + VRVI L RN GKG A+R G+  SRGE +LM DADGATK  D+EKL
Sbjct: 117 SKVALKYCQKYGSDKVRVITLVRNRGKGGAVRMGVFSSRGEKILMADADGATKFPDVEKL 176

Query: 178 ENQIHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLT 237
           E  +          D       + I A GSRAHLE+ ++A R ++R FLM GFH +V   
Sbjct: 177 EKGL---------SDLQPWPEQMAI-ACGSRAHLEKESIAQRSYFRTFLMYGFHFLVWFL 236

Query: 238 AGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIP 297
              GIRDTQCGFK+ TR AA + F ++ ++RW FDVEL+Y+++   IP+ EV+VNW+EI 
Sbjct: 237 CVKGIRDTQCGFKLLTREAAARTFSSLHIERWAFDVELLYIAQCLQIPIAEVAVNWTEIE 296

Query: 298 GSKLSP-RSILNMLWELALMSIGYKTGLWKI 328
           GSKL P  S L M  +L  + + Y TG W++
Sbjct: 297 GSKLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of CSPI05G17740 vs. ExPASy Swiss-Prot
Match: A2DZE8 (Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=5722 GN=ALG5A PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 123/279 (44.09%), Postives = 184/279 (65.95%), Query Frame = 0

Query: 49  PNSLKRVPCPSIFDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVII 108
           PN  ++VP P+IFDPA  Y+SL+VPA+NEE RL   L+ET+NYL+ R  KDKSF++E++I
Sbjct: 56  PNGNEKVPFPTIFDPASVYLSLVVPAYNEEKRLPKMLDETLNYLKSREEKDKSFTWEIVI 115

Query: 109 IDDGSADGTKGIAFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGA 168
           ++DGS D TK +  N+ +++   N+ ++    N GKG AI+ G LH RGE++LMLDADGA
Sbjct: 116 VNDGSKDKTKEVVLNYAKEY--PNIFLLNQPVNMGKGAAIQAGCLHVRGELVLMLDADGA 175

Query: 169 TKITDLEKLENQIHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMK 228
           TKI D E LE +I +++K  T+  + V          GSRA   +N  A R   R FL  
Sbjct: 176 TKIDDFEVLEKEIKSLMK--TTNQAIV---------IGSRA---QNEKAKRTPLRKFLSI 235

Query: 229 GFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIE 288
           G H +++L+   GIRDTQCGFK+FTR + + +F+N  ++RWC D E++ +++  G+ + E
Sbjct: 236 GMHTLIVLSGVHGIRDTQCGFKLFTRESCKMIFMNQHVQRWCCDPEILVIARRLGMKISE 295

Query: 289 VSVNWSEIPGSKLSPRSILNMLWELALMSIGYKTGLWKI 328
           + V W+EI GSK+    ++ M  +L  ++I ++ G WKI
Sbjct: 296 LPVEWNEIDGSKMKISGMIKMATDLIKIAIFHRVGAWKI 318

BLAST of CSPI05G17740 vs. ExPASy Swiss-Prot
Match: Q54J42 (Dolichyl-phosphate beta-glucosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg5 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 118/287 (41.11%), Postives = 181/287 (63.07%), Query Frame = 0

Query: 43  PAIFEDPNSLKRVPCPSIFDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSF 102
           P++ E+ N +  +           Y+S+IVPA+NE+ RL   L++ I +L +++ KD  F
Sbjct: 62  PSLVENKNPIDNI-----------YLSVIVPAYNEQIRLPSMLDDAIKFLNEKSKKDLKF 121

Query: 103 SYEVIIIDDGSADGTKGIAFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLM 162
           SYE+IIIDDGS D T  +  +++ K    N+R++ L +N GKG A+++G+L SRG+  LM
Sbjct: 122 SYEIIIIDDGSKDSTAKLVTSYIEKQPSSNIRLLKLKQNRGKGGAVKRGILCSRGKYCLM 181

Query: 163 LDADGATKITDLEKLENQIHAVVKRETSRDSSVRVSDIPITAFGSRAHL-EENALATRKW 222
           +DADGAT+  D  ++E+ +H + K           +D+ I   GSR+HL + + +A R +
Sbjct: 182 VDADGATEFKDFNRVEDIMHKIEK-----------NDLGIVC-GSRSHLVDSDLVAKRSF 241

Query: 223 YRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKY 282
            RN LM GFH+ V      GI+DTQCGFK+FTR  AR++F  + ++RW FDVE++YL++ 
Sbjct: 242 LRNILMYGFHIFVQTLCVKGIKDTQCGFKLFTRETARRIFPTLHIERWAFDVEILYLAQK 301

Query: 283 FGIPMIEVSVNWSEIPGSKLSP-RSILNMLWELALMSIGYKTGLWKI 328
             IP+ EV+VNW+EI GSKL P  S + M  ++  +   Y  G+WKI
Sbjct: 302 LNIPIAEVAVNWTEIDGSKLDPFSSSIQMAKDIVRIRFRYLLGIWKI 325

BLAST of CSPI05G17740 vs. ExPASy TrEMBL
Match: A0A0A0KSA3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G517110 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.6e-183
Identity = 323/329 (98.18%), Postives = 328/329 (99.70%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILFIFISLALCFPAAL+STLIIEAYKRRFNREH+GAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFIFISLALCFPAALLSTLIIEAYKRRFNREHLGAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAVVK+ETSRDSSVRVSDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAVVKKETSRDSSVRVSDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKIFN 330
           LSP+SILNMLWELALMSIGYKTGLWKIFN
Sbjct: 301 LSPQSILNMLWELALMSIGYKTGLWKIFN 329

BLAST of CSPI05G17740 vs. ExPASy TrEMBL
Match: A0A1S4E276 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498152 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 309/329 (93.92%), Postives = 318/329 (96.66%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEY WTILFIFISLALCF AA+IS LIIEA+KRRFNREHV APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYLWTILFIFISLALCFAAAIISALIIEAFKRRFNREHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL GALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVE VRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVEKVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAV K+ETSRDSS+++SDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLL AGP
Sbjct: 181 IHAVAKKETSRDSSIKISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLIAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVEL YLSKYFGIPMIEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELFYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKIFN 330
           LSPRSILNMLWELALMSIGYK G+W+IFN
Sbjct: 301 LSPRSILNMLWELALMSIGYKIGIWRIFN 329

BLAST of CSPI05G17740 vs. ExPASy TrEMBL
Match: A0A6J1GQI8 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456187 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF FISLAL   AALIST+I+EAY+RRFN  H+ APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL+GALEETINYLQQRAAKDKSFSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTA 240
           IH  AVVK+E SRD S+R+SDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSIGYKTGLWKI 328
           SKLSPRSILNMLWELALMS+GY+TG W+I
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRI 329

BLAST of CSPI05G17740 vs. ExPASy TrEMBL
Match: A0A6J1JRS3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487753 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.7e-166
Identity = 294/329 (89.36%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYP TILFI ISLAL   AALIS +I+EAY+RRFN  H+ APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPRTILFILISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL+GALEETINYLQQRAAKDKSFSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTA 240
           IH  AVVK+E SRD S+R+SDIPITAFGSRAHL+E ALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLKEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSIGYKTGLWKI 328
           SKLSPRSILNMLWELALMS+GY+TG+W+I
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGIWRI 329

BLAST of CSPI05G17740 vs. ExPASy TrEMBL
Match: A0A6J1C019 (Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111006250 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 280/327 (85.63%), Postives = 303/327 (92.66%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTI FI ISL   F A L+S +I++AY+RRFN EHV APAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYL+QRAAKDK+FSYEV+I+DDGS D TKG+
Sbjct: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFN VRK+SVENVRVILLG+NHGKGEA RKGMLHSRGE+LLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHA VK+E SRDSSVR+SD+PITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAEVKKEASRDSSVRISDVPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGIPM+EVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKI 328
           L P SILNMLWELALM++GY+TG+W+I
Sbjct: 301 LGPWSILNMLWELALMTVGYRTGIWRI 327

BLAST of CSPI05G17740 vs. NCBI nr
Match: XP_031741063.1 (dolichyl-phosphate beta-glucosyltransferase [Cucumis sativus] >KGN51312.1 hypothetical protein Csa_007869 [Cucumis sativus])

HSP 1 Score: 651.7 bits (1680), Expect = 3.3e-183
Identity = 323/329 (98.18%), Postives = 328/329 (99.70%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILFIFISLALCFPAAL+STLIIEAYKRRFNREH+GAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFIFISLALCFPAALLSTLIIEAYKRRFNREHLGAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAVVK+ETSRDSSVRVSDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAVVKKETSRDSSVRVSDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKIFN 330
           LSP+SILNMLWELALMSIGYKTGLWKIFN
Sbjct: 301 LSPQSILNMLWELALMSIGYKTGLWKIFN 329

BLAST of CSPI05G17740 vs. NCBI nr
Match: XP_016902331.1 (PREDICTED: dolichyl-phosphate beta-glucosyltransferase-like [Cucumis melo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.3e-175
Identity = 309/329 (93.92%), Postives = 318/329 (96.66%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEY WTILFIFISLALCF AA+IS LIIEA+KRRFNREHV APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYLWTILFIFISLALCFAAAIISALIIEAFKRRFNREHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL GALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVE VRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVEKVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAV K+ETSRDSS+++SDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLL AGP
Sbjct: 181 IHAVAKKETSRDSSIKISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLIAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVEL YLSKYFGIPMIEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELFYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKIFN 330
           LSPRSILNMLWELALMSIGYK G+W+IFN
Sbjct: 301 LSPRSILNMLWELALMSIGYKIGIWRIFN 329

BLAST of CSPI05G17740 vs. NCBI nr
Match: XP_038889980.1 (dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida])

HSP 1 Score: 607.8 bits (1566), Expect = 5.4e-170
Identity = 296/329 (89.97%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILFI ISLALC    LIS +IIEAY+RRFN +HV APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFILISLALCIAVGLISAVIIEAYRRRFNHQHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL GALEETINYLQQRAAKDKS+SYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSYSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAVVK+E SRD S+R+SDIPI AFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAVVKKEASRDPSIRISDIPIAAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSA RKLF NIRLKRWCFDVELVYL K+FGIPMIEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAGRKLFSNIRLKRWCFDVELVYLCKFFGIPMIEVSVNWSEIPGSK 300

Query: 301 LSPRSILNMLWELALMSIGYKTGLWKIFN 330
           LSPRSILNMLWELALMS+GY+T +W+IFN
Sbjct: 301 LSPRSILNMLWELALMSVGYRTRIWRIFN 329

BLAST of CSPI05G17740 vs. NCBI nr
Match: XP_022953750.1 (dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 601.7 bits (1550), Expect = 3.9e-168
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF FISLAL   AALIST+I+EAY+RRFN  H+ APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL+GALEETINYLQQRAAKDKSFSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AVVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTA 240
           IH  AVVK+E SRD S+R+SDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSIGYKTGLWKI 328
           SKLSPRSILNMLWELALMS+GY+TG W+I
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRI 329

BLAST of CSPI05G17740 vs. NCBI nr
Match: KAG7033054.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 598.2 bits (1541), Expect = 4.3e-167
Identity = 295/329 (89.67%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF FISLAL   AALIS +I+EAY+RRFN  H+ APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
           FDPAEKYMSLIVPAFNEEYRL+GALEETINYL+QRAAKDKSFSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLRQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AFNFVRK+SVENVRVILLGRNHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHA--VVKRETSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVVLLTA 240
           IHA  VVK+E SRD S+R+SDIPITAFGSRAHLEE ALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVVVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSIGYKTGLWKI 328
           SKLSPRSILNMLWELALMS+GY+TG W+I
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRI 329

BLAST of CSPI05G17740 vs. TAIR 10
Match: AT2G39630.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.2e-129
Identity = 220/333 (66.07%), Postives = 270/333 (81.08%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           ME+  T+    + L L      +S ++ EA++RR +   V      +DP S+K +PCP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
            DPAEKY+SLIVPA+NEE RL  ALEET++YLQ RA++DKSFS+EV+I+DDGS DGTK +
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AF+F+RK++++N+RVI LG+N GKGEAIRKGMLHSRG++LLMLDADGATK+TDLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAVVKRE------TSRDSSVRVSDIPITAFGSRAHLEENALATRKWYRNFLMKGFHLVV 240
           I+AV + E       S+D   ++ D+ ++AFGSRAHLEE ALATRKWYRNFLMKGFHLVV
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALATRKWYRNFLMKGFHLVV 240

Query: 241 LLTAGPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWS 300
           LL AGPGIRDTQCGFKMFTR+AAR+LF N+ LKRWCFDVELVYL K F IPM+E+SV WS
Sbjct: 241 LLAAGPGIRDTQCGFKMFTRAAARRLFTNVHLKRWCFDVELVYLCKRFNIPMVEISVKWS 300

Query: 301 EIPGSKLSPRSILNMLWELALMSIGYKTGLWKI 328
           EIPGSK+S  SI NMLWELALMS+GY+TG+WKI
Sbjct: 301 EIPGSKVSMLSIPNMLWELALMSVGYRTGMWKI 333

BLAST of CSPI05G17740 vs. TAIR 10
Match: AT2G39630.2 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-70
Identity = 130/224 (58.04%), Postives = 171/224 (76.34%), Query Frame = 0

Query: 1   MEYPWTILFIFISLALCFPAALISTLIIEAYKRRFNREHVGAPAIFEDPNSLKRVPCPSI 60
           ME+  T+    + L L      +S ++ EA++RR +   V      +DP S+K +PCP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120
            DPAEKY+SLIVPA+NEE RL  ALEET++YLQ RA++DKSFS+EV+I+DDGS DGTK +
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 AFNFVRKHSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180
           AF+F+RK++++N+RVI LG+N GKGEAIRKGMLHSRG++LLMLDADGATK+TDLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAVVKRE------TSRDSSVRVSDIPITAFGSRAHLEENALAT 219
           I+AV + E       S+D   ++ D+ ++AFGSRAHLEE ALAT
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALAT 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9VLQ11.4e-6744.04Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 G... [more]
Q9Y6732.0e-6647.23Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1... [more]
Q9DB251.4e-6447.23Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=... [more]
A2DZE81.8e-6244.09Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=57... [more]
Q54J421.3e-6041.11Dolichyl-phosphate beta-glucosyltransferase OS=Dictyostelium discoideum OX=44689... [more]
Match NameE-valueIdentityDescription
A0A0A0KSA31.6e-18398.18Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S4E2762.1e-17593.92Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A6J1GQI81.9e-16890.27Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JRS36.7e-16689.36Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1C0191.2e-16285.63Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_031741063.13.3e-18398.18dolichyl-phosphate beta-glucosyltransferase [Cucumis sativus] >KGN51312.1 hypoth... [more]
XP_016902331.14.3e-17593.92PREDICTED: dolichyl-phosphate beta-glucosyltransferase-like [Cucumis melo][more]
XP_038889980.15.4e-17089.97dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida][more]
XP_022953750.13.9e-16890.27dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata][more]
KAG7033054.14.3e-16789.67Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT2G39630.12.2e-12966.07Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT2G39630.21.1e-7058.04Nucleotide-diphospho-sugar transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001173Glycosyltransferase 2-likePFAMPF00535Glycos_transf_2coord: 69..181
e-value: 1.2E-21
score: 77.2
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 57..314
e-value: 1.0E-32
score: 115.7
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 65..316
NoneNo IPR availablePANTHERPTHR10859:SF108BNAA03G18660D PROTEINcoord: 21..328
NoneNo IPR availablePANTHERPTHR10859GLYCOSYL TRANSFERASEcoord: 21..328
IPR035518Dolichyl-phosphate beta-glucosyltransferaseCDDcd04188DPG_synthasecoord: 70..300
e-value: 3.3571E-102
score: 297.171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G17740.1CSPI05G17740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004581 dolichyl-phosphate beta-glucosyltransferase activity