CSPI05G15640 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G15640
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCation/H(+) antiporter 4-like
LocationChr5: 16696219 .. 16697283 (-)
RNA-Seq ExpressionCSPI05G15640
SyntenyCSPI05G15640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTAAGTGTGGTTAAAAGATCAGGCAGGCAATCTTTGATAGGTGGTATTTTATCAATAGTTATTCCTGCAATACTTGGTTCGTTGACAGCATTTGGTTTATCAAGATTTAGTAAAACACATGGAACGGCTGACATGGAGTTTATAGCCGCACACCAATCATACACTTCATTTGCTGTTATGGTTTGCCTTCTTGACCATCTCAAAATTCTGAATTCTGAAGTTGGTCGTTTGGTACTTTCTACTACAATTGTAGCTGATTTGGTGGGTCTGAGTTTCTCCTTGATAATTACTGTTATTGAAAATGTTCGAAGCCAGGGTGCTTTGAATGGCTTGATGACTTTTGCTATGGCGATTGGATCGTTGGTCCTTATTGTGTTTTTGTTTCGTCCTGCGATGCTCTGGATTGTTAGGTCTACACCAAGTGGGAGGCCTGTGCCGGATGGATACATATGCATCATCATTCTATTGGTGCTTGTATCTAGTGTAACTTCTAACATTATGGGACGAACCGTTTATTCAGGACCATTTATCTTGGGTTTGACTGTGCCTGAAGGACCACCTTTAGGAGTCAGTCTAGTGAAGAAACTGGACAGCATTATTACATCGGTTTTCGTTCCGCTCTTTGTCACTATTTCTGTGATGAAGGTTGATCTTTCATTCCTTTACTACGATGGAGAATTTTTGATCCATTCTATTATTGTGATCTTTATATCCAGTATTGGAAAATTGGCTGTTTCTGTTGGTACTGCTCTATATTTCAAGATGTCTTCTCATGATGCCTTGGCATTTGGCCTCATAATGTGTAGCAAAGGCATTGTAGAGCTTGCTGCTTGCTCTTACTTTTATGACAGCAATGTAAGGGTTTACATAACATTAGATTTATATATATATATATATATATATATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGAGGTATAAGTTTCATTGTATTATTACTTATTACTATTAA

mRNA sequence

ATGGATTTAAGTGTGGTTAAAAGATCAGGCAGGCAATCTTTGATAGGTGGTATTTTATCAATAGTTATTCCTGCAATACTTGGTTCGTTGACAGCATTTGGTTTATCAAGATTTAGTAAAACACATGGAACGGCTGACATGGAGTTTATAGCCGCACACCAATCATACACTTCATTTGCTGTTATGGTTTGCCTTCTTGACCATCTCAAAATTCTGAATTCTGAAGTTGGTCGTTTGGTACTTTCTACTACAATTGTAGCTGATTTGGTGGGTCTGAGTTTCTCCTTGATAATTACTGTTATTGAAAATGTTCGAAGCCAGGGTGCTTTGAATGGCTTGATGACTTTTGCTATGGCGATTGGATCGTTGGTCCTTATTGTGTTTTTGTTTCGTCCTGCGATGCTCTGGATTGTTAGGTCTACACCAAGTGGGAGGCCTGTGCCGGATGGATACATATGCATCATCATTCTATTGGTGCTTGTATCTAGTGTAACTTCTAACATTATGGGACGAACCGTTTATTCAGGACCATTTATCTTGGGTTTGACTGTGCCTGAAGGACCACCTTTAGGAGTCAGTCTAGTGAAGAAACTGGACAGCATTATTACATCGGTTTTCGTTCCGCTCTTTGTCACTATTTCTGTGATGAAGGTTGATCTTTCATTCCTTTACTACGATGGAGAATTTTTGATCCATTCTATTATTGTGATCTTTATATCCAGTATTGGAAAATTGGCTGTTTCTGTTGGTACTGCTCTATATTTCAAGATGTCTTCTCATGATGCCTTGGCATTTGGCCTCATAATGTGTAGCAAAGGCATTGTAGAGCTTGCTGCTTGCTCTTACTTTTATGACAGCAATGTAAGGGTTTACATAACATTAGATTTATATATATATATATATATATATATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGAGGTATAAGTTTCATTGTATTATTACTTATTACTATTAA

Coding sequence (CDS)

ATGGATTTAAGTGTGGTTAAAAGATCAGGCAGGCAATCTTTGATAGGTGGTATTTTATCAATAGTTATTCCTGCAATACTTGGTTCGTTGACAGCATTTGGTTTATCAAGATTTAGTAAAACACATGGAACGGCTGACATGGAGTTTATAGCCGCACACCAATCATACACTTCATTTGCTGTTATGGTTTGCCTTCTTGACCATCTCAAAATTCTGAATTCTGAAGTTGGTCGTTTGGTACTTTCTACTACAATTGTAGCTGATTTGGTGGGTCTGAGTTTCTCCTTGATAATTACTGTTATTGAAAATGTTCGAAGCCAGGGTGCTTTGAATGGCTTGATGACTTTTGCTATGGCGATTGGATCGTTGGTCCTTATTGTGTTTTTGTTTCGTCCTGCGATGCTCTGGATTGTTAGGTCTACACCAAGTGGGAGGCCTGTGCCGGATGGATACATATGCATCATCATTCTATTGGTGCTTGTATCTAGTGTAACTTCTAACATTATGGGACGAACCGTTTATTCAGGACCATTTATCTTGGGTTTGACTGTGCCTGAAGGACCACCTTTAGGAGTCAGTCTAGTGAAGAAACTGGACAGCATTATTACATCGGTTTTCGTTCCGCTCTTTGTCACTATTTCTGTGATGAAGGTTGATCTTTCATTCCTTTACTACGATGGAGAATTTTTGATCCATTCTATTATTGTGATCTTTATATCCAGTATTGGAAAATTGGCTGTTTCTGTTGGTACTGCTCTATATTTCAAGATGTCTTCTCATGATGCCTTGGCATTTGGCCTCATAATGTGTAGCAAAGGCATTGTAGAGCTTGCTGCTTGCTCTTACTTTTATGACAGCAATGTAAGGGTTTACATAACATTAGATTTATATATATATATATATATATATATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGAGGTATAAGTTTCATTGTATTATTACTTATTACTATTAA

Protein sequence

MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAIGSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNVRVYITLDLYIYIYIYIYIYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLRYKFHCIITYYY*
Homology
BLAST of CSPI05G15640 vs. ExPASy Swiss-Prot
Match: Q3EDG3 (Cation/H(+) antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=CHX5 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.0e-32
Identity = 101/298 (33.89%), Postives = 165/298 (55.37%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEF--IAAHQSYTS 60
           MD+ +V+++G + ++ GI ++++P I  ++  FG  R +       ME+  I   QS ++
Sbjct: 151 MDVGMVRKTGTKVIVTGIATVILPIIAANM-VFGKLRETGGKYLTGMEYRTILFMQSISA 210

Query: 61  FAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAM 120
           F  +  LL  L+I +SE GR+V+ST +VAD  G   +L   V        AL G+     
Sbjct: 211 FTGISRLLRDLRINHSEFGRIVISTAMVADGTGFGVNLFALVAWMDWRVSALQGV----G 270

Query: 121 AIGSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPF 180
            IG ++ +V++ RPAM W+++ TP  RPV + +I II++L          +      GPF
Sbjct: 271 IIGYVIFMVWVVRPAMFWVIKRTPQERPVKECFIYIILILAFGGYYFLKEIHMFPAVGPF 330

Query: 181 ILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFL--------YYDGEFL 240
           +LGL VP GPPLG  LV+K +S  T + +PLF+  S++++D  +L        ++DG+ L
Sbjct: 331 LLGLCVPHGPPLGSQLVEKFESFNTGILLPLFLFFSMLQIDGPWLANQIGQLRHFDGQ-L 390

Query: 241 IHSIIVIFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
             ++ +I +  + K+  S+  AL  KM   D+    LI+ +KGIVEL    Y  +SNV
Sbjct: 391 YEALTIIIVVFVAKIIFSMIPALLAKMPLTDSFVMALILSNKGIVELCYFLYGVESNV 442

BLAST of CSPI05G15640 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.7e-32
Identity = 104/324 (32.10%), Postives = 177/324 (54.63%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAF------GLSRFSKTHGTADMEFIAAHQ 60
           MDLS+++ +GR+++  G+ S+++   + +L  F      G  +        ++ FI   Q
Sbjct: 137 MDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFIYLIQ 196

Query: 61  SYTSFAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLM 120
             +SF V+  LL  L++ NSE+GRL +S+ +++D      S ++  ++ ++   +  G +
Sbjct: 197 CLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSRLGSV 256

Query: 121 TFAMAI---------GSLVLIV----FLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLV 180
                I         G++VL V    ++FRP M +I++ TPSGRPV   YI  II+LV  
Sbjct: 257 FIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIIILVFG 316

Query: 181 SSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLS 240
           S++ ++   ++++ GPFILGL VP GPPLG ++++K +S++   F+P FV  S  ++D S
Sbjct: 317 SAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAEEIDTS 376

Query: 241 FL--YYDGEFLIHSIIVIFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAA 300
            L  + D   L   +I++ +S I K A++   A  + M + D +A  LIM  KGI E  A
Sbjct: 377 ILQSWID---LKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEFGA 436

BLAST of CSPI05G15640 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 7.7e-32
Identity = 104/337 (30.86%), Postives = 187/337 (55.49%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKT----HGTADMEFIAAH--Q 60
           MD  +++ +GR+++  G+ S+++  ++ S+  FG  R   T    H    +E++  +  Q
Sbjct: 138 MDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQ 197

Query: 61  SYTSFAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNG-- 120
             +SF V+  LL  L++ NSE+GRL +S+ +++D      + ++  ++ ++ +    G  
Sbjct: 198 CLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRLGSV 257

Query: 121 -----------LMTFAMAIGSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLV 180
                      LM   + +  + + +++FRP M +I++ TPSGRPV   Y+  II++V  
Sbjct: 258 FIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSG 317

Query: 181 SSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLS 240
           S++ +N   ++++ GPFILGL VP GPPLG ++++K +S I   F+P F+  S  ++D+S
Sbjct: 318 SAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEIDIS 377

Query: 241 FLY-YDGEFLIHSIIVIFISS-IGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAA 300
            L+ ++G   ++ II+I ++S + K   +   AL++ M   D  A  LIM  KGI EL A
Sbjct: 378 ALFGWEG---LNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGA 437

Query: 301 CSYFYD-SNVR--------VYITLDLYIYIYIYIYIY 308
            +  Y   +VR        +YITL+  I   I  Y+Y
Sbjct: 438 YALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLY 471

BLAST of CSPI05G15640 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.6e-29
Identity = 85/292 (29.11%), Postives = 162/292 (55.48%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTAD-MEFIAAHQSYTSF 60
           MD+ VV+++G+++L   I  +V+P ++G+  +F + R     G    + F+    S T+F
Sbjct: 119 MDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAF 178

Query: 61  AVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMA 120
            V+  +L  LK++N+E+GR+ +S  +V D+    F+ I+  +    ++       +  + 
Sbjct: 179 PVLARILAELKLINTEIGRISMSAALVNDM----FAWILLALAIALAESDKTSFASLWVM 238

Query: 121 IGSLVLI---VFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSG 180
           I S V I   VF+ RP + WI+R TP G    + +IC+I+  V++S   ++ +G     G
Sbjct: 239 ISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFG 298

Query: 181 PFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIV 240
            F+ GL +P G PLG++L++KL+  ++ + +PLF  IS +K +++ +     +L    +V
Sbjct: 299 AFVFGLVIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLT-LFLV 358

Query: 241 IFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           IF++  GK+  +V  A +  M   + +  GL++ +KG+VE+   +   D  V
Sbjct: 359 IFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKV 404

BLAST of CSPI05G15640 vs. ExPASy Swiss-Prot
Match: Q9FYC0 (Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 5.2e-28
Identity = 90/303 (29.70%), Postives = 157/303 (51.82%), Query Frame = 0

Query: 9   SGRQSLIGGILSIVIPAILGSLTAFGLSR-------FSKTHGTADMEFIAAHQSYTSFAV 68
           +G   ++ G LS ++P  LG      L          S     A+   + + QS      
Sbjct: 130 NGWLPIVIGTLSFIVP-FLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISSQSSILLPT 189

Query: 69  MVCLLDHLKILNSEVGRLVLSTTIVADLVGLS---FSLIITVIENVRSQGALNGLMTFAM 128
           +V  L  LKILNSE+GRLVLS +++ D+   +   F+ ++   +N+    A   L    +
Sbjct: 190 VVHFLSELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTAYRDL----I 249

Query: 129 AIGSLVLIVF-LFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGP 188
           A+  L+L+ F + RP + WIV  TP G+PV D Y+  ++L V+ S+  S+        GP
Sbjct: 250 AVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKYLLGP 309

Query: 189 FILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVI 248
           F+LG+ +PEGPP+G +L  K +++  +V +P+ +T S M+ D+  + Y  + + ++I ++
Sbjct: 310 FLLGIIIPEGPPIGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWYNIFLM 369

Query: 249 FISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNVRVYITLDLY 301
             +   K+A  +   LY K+   +A+A  L++CSK   E+      YD +   YI+   Y
Sbjct: 370 TFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDS---YISQATY 424

BLAST of CSPI05G15640 vs. ExPASy TrEMBL
Match: A0A0A0KN98 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G450650 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 285/287 (99.30%), Postives = 285/287 (99.30%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA
Sbjct: 55  MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 114

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI
Sbjct: 115 VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 174

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL
Sbjct: 175 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 234

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GLTVPEGPPLG SLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS
Sbjct: 235 GLTVPEGPPLGASLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 294

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSN 288
           SIGKL VSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSN
Sbjct: 295 SIGKLVVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSN 341

BLAST of CSPI05G15640 vs. ExPASy TrEMBL
Match: A0A5D3BL54 (Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold446G00720 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.7e-133
Identity = 256/288 (88.89%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGG+LSIVIPAILGSLTAFG SR  KTH TA+MEF+AA+QSYTSFA
Sbjct: 136 MDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETANMEFVAANQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+VCLLDHLKILNSEVGRLVLSTTIVADLVGLSFS I+TV+ENV+SQGAL+GLMT A+A+
Sbjct: 196 VVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIVTVVENVQSQGALSGLMTIALAV 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GS+VL+VFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSS TSNIMGRTVYSGPFIL
Sbjct: 256 GSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSATSNIMGRTVYSGPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV KLDSIITSVFVPLFVTI VMKVDLSFL YDGEF I+S IVIFIS
Sbjct: 316 GLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLYDGEFFIYSTIVIFIS 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           +IGKLAVSVGTALYFKMSSHDALAFGLIMC+KGIVELAACS+FYDSN+
Sbjct: 376 TIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYDSNL 423

BLAST of CSPI05G15640 vs. ExPASy TrEMBL
Match: A0A1S3AUL2 (cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103483015 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.0e-133
Identity = 256/288 (88.89%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGG+LSIVIPAILGSLTAFG SR  KTH TA+MEF+AA+QSYTSFA
Sbjct: 136 MDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETANMEFVAANQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+VCLLDHLKILNSEVGRLVLSTTIVADLVGLSFS IITV+ENV+SQGAL+GLMT A+A+
Sbjct: 196 VVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIITVVENVQSQGALSGLMTIALAV 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GS+VL+VFLFRPAMLWIVRSTPSGRPVPDGYICIII+LVLVSS TSNIMGRTVYSGPFIL
Sbjct: 256 GSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIIVLVLVSSATSNIMGRTVYSGPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV KLDSIITSVFVPLFVTI VMKVDLSFL YDGEF I+S IVIFIS
Sbjct: 316 GLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLYDGEFFIYSTIVIFIS 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           +IGKLAVSVGTALYFKMSSHDALAFGLIMC+KGIVELAACS+FYDSN+
Sbjct: 376 TIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYDSNL 423

BLAST of CSPI05G15640 vs. ExPASy TrEMBL
Match: A0A6J1DUA7 (cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111024501 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 2.3e-103
Identity = 209/306 (68.30%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDL VVKRSGRQ+LI G+LSI++PA+LG + A GLSRF +    A++EFIAA+QSYTSFA
Sbjct: 136 MDLGVVKRSGRQTLITGVLSIIVPALLGLMAAVGLSRFGEEDEAANLEFIAANQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+V LL+HLKILNSEVGRLVLS++IVAD+VGLSFS I++V+ENV S G     + F   I
Sbjct: 196 VVVSLLEHLKILNSEVGRLVLSSSIVADMVGLSFSFIVSVVENVHSHGVFGASIGFIFTI 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
            S+V+++F+FRP MLWIVRSTP GRPV DGYIC+IILLVLVSSVTSNIMGRT+YSGPF+L
Sbjct: 256 LSVVIVLFVFRPVMLWIVRSTPHGRPVQDGYICLIILLVLVSSVTSNIMGRTIYSGPFLL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV KLD IITSVF+PLF+TI+V+K DLSF+ Y G FL  S+ VI I+
Sbjct: 316 GLVVPEGPPLGASLVNKLDGIITSVFIPLFITIAVIKADLSFINYSGLFLAKSMTVILIT 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNVRVYITLDLYIYI 300
            +GK+AV VGT+LYFKMSS+DALAFGLIM SKGIVELAA SYFYDS V  + T  + +  
Sbjct: 376 IMGKMAVCVGTSLYFKMSSYDALAFGLIMSSKGIVELAASSYFYDSKVLSHQTFAVLVVD 435

Query: 301 YIYIYI 307
            + I I
Sbjct: 436 ILIISI 441

BLAST of CSPI05G15640 vs. ExPASy TrEMBL
Match: A0A6J1GPY8 (cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456471 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 1.4e-89
Identity = 188/286 (65.73%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDL+VVK+SG+Q LIGG+LS+VI AI+GS+TAF LSR  K     +ME+IAA QS+TSFA
Sbjct: 136 MDLNVVKKSGKQPLIGGVLSVVISAIIGSITAFSLSRVDKRGELINMEYIAAAQSFTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+  LLDHLKILNSEVGRL LSTTIVADL  LS S I T I +V+  G L   M+F   I
Sbjct: 196 VVHYLLDHLKILNSEVGRLALSTTIVADLTSLSISFIATFIRSVQIHGVLKASMSFTSTI 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GS+V ++F+FRPAML I RSTP+GRPV D YI II+LLV VS  T   +GR+ YS PFIL
Sbjct: 256 GSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTHVTIGRSAYSAPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV +LD IITSVFVPLFVTI+VMK DLSFL Y  +FL  S IVI ++
Sbjct: 316 GLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLTYSTKFLARSTIVIIMT 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDS 287
           ++ K+  SVGT+LYFKMSS+DALAFG IM SKGI+EL   S+FYDS
Sbjct: 376 TVAKMIASVGTSLYFKMSSYDALAFGFIMSSKGIIELVGSSFFYDS 421

BLAST of CSPI05G15640 vs. NCBI nr
Match: XP_031741739.1 (cation/H(+) antiporter 4-like [Cucumis sativus])

HSP 1 Score: 530.8 bits (1366), Expect = 9.1e-147
Identity = 286/288 (99.31%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA
Sbjct: 108 MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 167

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI
Sbjct: 168 VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 227

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL
Sbjct: 228 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 287

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GLTVPEGPPLG SLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS
Sbjct: 288 GLTVPEGPPLGASLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 347

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSN+
Sbjct: 348 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNL 395

BLAST of CSPI05G15640 vs. NCBI nr
Match: KAE8648452.1 (hypothetical protein Csa_007933 [Cucumis sativus])

HSP 1 Score: 530.8 bits (1366), Expect = 9.1e-147
Identity = 286/288 (99.31%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA
Sbjct: 136 MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI
Sbjct: 196 VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL
Sbjct: 256 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GLTVPEGPPLG SLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS
Sbjct: 316 GLTVPEGPPLGASLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSN+
Sbjct: 376 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNL 423

BLAST of CSPI05G15640 vs. NCBI nr
Match: KAA0066038.1 (cation/H(+) antiporter 4-like [Cucumis melo var. makuwa] >TYJ99857.1 cation/H(+) antiporter 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 484.2 bits (1245), Expect = 9.7e-133
Identity = 256/288 (88.89%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGG+LSIVIPAILGSLTAFG SR  KTH TA+MEF+AA+QSYTSFA
Sbjct: 136 MDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETANMEFVAANQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+VCLLDHLKILNSEVGRLVLSTTIVADLVGLSFS I+TV+ENV+SQGAL+GLMT A+A+
Sbjct: 196 VVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIVTVVENVQSQGALSGLMTIALAV 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GS+VL+VFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSS TSNIMGRTVYSGPFIL
Sbjct: 256 GSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSATSNIMGRTVYSGPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV KLDSIITSVFVPLFVTI VMKVDLSFL YDGEF I+S IVIFIS
Sbjct: 316 GLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLYDGEFFIYSTIVIFIS 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           +IGKLAVSVGTALYFKMSSHDALAFGLIMC+KGIVELAACS+FYDSN+
Sbjct: 376 TIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYDSNL 423

BLAST of CSPI05G15640 vs. NCBI nr
Match: XP_008437675.1 (PREDICTED: cation/H(+) antiporter 4-like [Cucumis melo])

HSP 1 Score: 483.4 bits (1243), Expect = 1.7e-132
Identity = 256/288 (88.89%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFA 60
           MDLSVVKRSGRQSLIGG+LSIVIPAILGSLTAFG SR  KTH TA+MEF+AA+QSYTSFA
Sbjct: 136 MDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETANMEFVAANQSYTSFA 195

Query: 61  VMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAI 120
           V+VCLLDHLKILNSEVGRLVLSTTIVADLVGLSFS IITV+ENV+SQGAL+GLMT A+A+
Sbjct: 196 VVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIITVVENVQSQGALSGLMTIALAV 255

Query: 121 GSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFIL 180
           GS+VL+VFLFRPAMLWIVRSTPSGRPVPDGYICIII+LVLVSS TSNIMGRTVYSGPFIL
Sbjct: 256 GSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIIVLVLVSSATSNIMGRTVYSGPFIL 315

Query: 181 GLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFIS 240
           GL VPEGPPLG SLV KLDSIITSVFVPLFVTI VMKVDLSFL YDGEF I+S IVIFIS
Sbjct: 316 GLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLYDGEFFIYSTIVIFIS 375

Query: 241 SIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           +IGKLAVSVGTALYFKMSSHDALAFGLIMC+KGIVELAACS+FYDSN+
Sbjct: 376 TIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYDSNL 423

BLAST of CSPI05G15640 vs. NCBI nr
Match: KGN51475.2 (hypothetical protein Csa_008150 [Cucumis sativus])

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-128
Identity = 255/272 (93.75%), Postives = 259/272 (95.22%), Query Frame = 0

Query: 17  GILSIVIPAILGSLTAFGLSRFSKTHGTADMEFIAAHQSYTSFAVMVCLLDHLKILNSEV 76
           G+ +     I+G L  FG   FSKTHGTADMEFIAAHQSYTSFAVMVCLLDHLKILNSEV
Sbjct: 107 GVFATASQEIVGLLAGFG---FSKTHGTADMEFIAAHQSYTSFAVMVCLLDHLKILNSEV 166

Query: 77  GRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAIGSLVLIVFLFRPAMLW 136
           GRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAIGSLVLIVFLFRPAMLW
Sbjct: 167 GRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMAIGSLVLIVFLFRPAMLW 226

Query: 137 IVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVK 196
           IVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFILGLTVPEGPPLG SLVK
Sbjct: 227 IVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPFILGLTVPEGPPLGASLVK 286

Query: 197 KLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFISSIGKLAVSVGTALYFK 256
           KLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFISSIGKLAVSVGTALYFK
Sbjct: 287 KLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVIFISSIGKLAVSVGTALYFK 346

Query: 257 MSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           MSSHDALAFGLIMCSKGIVELAACSYFYDSN+
Sbjct: 347 MSSHDALAFGLIMCSKGIVELAACSYFYDSNL 375

BLAST of CSPI05G15640 vs. TAIR 10
Match: AT1G08150.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-33
Identity = 101/298 (33.89%), Postives = 165/298 (55.37%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTADMEF--IAAHQSYTS 60
           MD+ +V+++G + ++ GI ++++P I  ++  FG  R +       ME+  I   QS ++
Sbjct: 151 MDVGMVRKTGTKVIVTGIATVILPIIAANM-VFGKLRETGGKYLTGMEYRTILFMQSISA 210

Query: 61  FAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAM 120
           F  +  LL  L+I +SE GR+V+ST +VAD  G   +L   V        AL G+     
Sbjct: 211 FTGISRLLRDLRINHSEFGRIVISTAMVADGTGFGVNLFALVAWMDWRVSALQGV----G 270

Query: 121 AIGSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGPF 180
            IG ++ +V++ RPAM W+++ TP  RPV + +I II++L          +      GPF
Sbjct: 271 IIGYVIFMVWVVRPAMFWVIKRTPQERPVKECFIYIILILAFGGYYFLKEIHMFPAVGPF 330

Query: 181 ILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFL--------YYDGEFL 240
           +LGL VP GPPLG  LV+K +S  T + +PLF+  S++++D  +L        ++DG+ L
Sbjct: 331 LLGLCVPHGPPLGSQLVEKFESFNTGILLPLFLFFSMLQIDGPWLANQIGQLRHFDGQ-L 390

Query: 241 IHSIIVIFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
             ++ +I +  + K+  S+  AL  KM   D+    LI+ +KGIVEL    Y  +SNV
Sbjct: 391 YEALTIIIVVFVAKIIFSMIPALLAKMPLTDSFVMALILSNKGIVELCYFLYGVESNV 442

BLAST of CSPI05G15640 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 141.0 bits (354), Expect = 1.9e-33
Identity = 104/324 (32.10%), Postives = 177/324 (54.63%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAF------GLSRFSKTHGTADMEFIAAHQ 60
           MDLS+++ +GR+++  G+ S+++   + +L  F      G  +        ++ FI   Q
Sbjct: 137 MDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFIYLIQ 196

Query: 61  SYTSFAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLM 120
             +SF V+  LL  L++ NSE+GRL +S+ +++D      S ++  ++ ++   +  G +
Sbjct: 197 CLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSRLGSV 256

Query: 121 TFAMAI---------GSLVLIV----FLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLV 180
                I         G++VL V    ++FRP M +I++ TPSGRPV   YI  II+LV  
Sbjct: 257 FIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIIILVFG 316

Query: 181 SSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLS 240
           S++ ++   ++++ GPFILGL VP GPPLG ++++K +S++   F+P FV  S  ++D S
Sbjct: 317 SAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAEEIDTS 376

Query: 241 FL--YYDGEFLIHSIIVIFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAA 300
            L  + D   L   +I++ +S I K A++   A  + M + D +A  LIM  KGI E  A
Sbjct: 377 ILQSWID---LKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEFGA 436

BLAST of CSPI05G15640 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 139.4 bits (350), Expect = 5.5e-33
Identity = 104/337 (30.86%), Postives = 187/337 (55.49%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKT----HGTADMEFIAAH--Q 60
           MD  +++ +GR+++  G+ S+++  ++ S+  FG  R   T    H    +E++  +  Q
Sbjct: 138 MDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQ 197

Query: 61  SYTSFAVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNG-- 120
             +SF V+  LL  L++ NSE+GRL +S+ +++D      + ++  ++ ++ +    G  
Sbjct: 198 CLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRLGSV 257

Query: 121 -----------LMTFAMAIGSLVLIVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLV 180
                      LM   + +  + + +++FRP M +I++ TPSGRPV   Y+  II++V  
Sbjct: 258 FIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSG 317

Query: 181 SSVTSNIMGRTVYSGPFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLS 240
           S++ +N   ++++ GPFILGL VP GPPLG ++++K +S I   F+P F+  S  ++D+S
Sbjct: 318 SAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEIDIS 377

Query: 241 FLY-YDGEFLIHSIIVIFISS-IGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAA 300
            L+ ++G   ++ II+I ++S + K   +   AL++ M   D  A  LIM  KGI EL A
Sbjct: 378 ALFGWEG---LNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGA 437

Query: 301 CSYFYD-SNVR--------VYITLDLYIYIYIYIYIY 308
            +  Y   +VR        +YITL+  I   I  Y+Y
Sbjct: 438 YALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLY 471

BLAST of CSPI05G15640 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-30
Identity = 85/292 (29.11%), Postives = 162/292 (55.48%), Query Frame = 0

Query: 1   MDLSVVKRSGRQSLIGGILSIVIPAILGSLTAFGLSRFSKTHGTAD-MEFIAAHQSYTSF 60
           MD+ VV+++G+++L   I  +V+P ++G+  +F + R     G    + F+    S T+F
Sbjct: 119 MDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAF 178

Query: 61  AVMVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSLIITVIENVRSQGALNGLMTFAMA 120
            V+  +L  LK++N+E+GR+ +S  +V D+    F+ I+  +    ++       +  + 
Sbjct: 179 PVLARILAELKLINTEIGRISMSAALVNDM----FAWILLALAIALAESDKTSFASLWVM 238

Query: 121 IGSLVLI---VFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSG 180
           I S V I   VF+ RP + WI+R TP G    + +IC+I+  V++S   ++ +G     G
Sbjct: 239 ISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFG 298

Query: 181 PFILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIV 240
            F+ GL +P G PLG++L++KL+  ++ + +PLF  IS +K +++ +     +L    +V
Sbjct: 299 AFVFGLVIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLT-LFLV 358

Query: 241 IFISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNV 289
           IF++  GK+  +V  A +  M   + +  GL++ +KG+VE+   +   D  V
Sbjct: 359 IFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKV 404

BLAST of CSPI05G15640 vs. TAIR 10
Match: AT3G44910.1 (cation/H+ exchanger 12 )

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-29
Identity = 90/303 (29.70%), Postives = 157/303 (51.82%), Query Frame = 0

Query: 9   SGRQSLIGGILSIVIPAILGSLTAFGLSR-------FSKTHGTADMEFIAAHQSYTSFAV 68
           +G   ++ G LS ++P  LG      L          S     A+   + + QS      
Sbjct: 130 NGWLPIVIGTLSFIVP-FLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISSQSSILLPT 189

Query: 69  MVCLLDHLKILNSEVGRLVLSTTIVADLVGLS---FSLIITVIENVRSQGALNGLMTFAM 128
           +V  L  LKILNSE+GRLVLS +++ D+   +   F+ ++   +N+    A   L    +
Sbjct: 190 VVHFLSELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTAYRDL----I 249

Query: 129 AIGSLVLIVF-LFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSVTSNIMGRTVYSGP 188
           A+  L+L+ F + RP + WIV  TP G+PV D Y+  ++L V+ S+  S+        GP
Sbjct: 250 AVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKYLLGP 309

Query: 189 FILGLTVPEGPPLGVSLVKKLDSIITSVFVPLFVTISVMKVDLSFLYYDGEFLIHSIIVI 248
           F+LG+ +PEGPP+G +L  K +++  +V +P+ +T S M+ D+  + Y  + + ++I ++
Sbjct: 310 FLLGIIIPEGPPIGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWYNIFLM 369

Query: 249 FISSIGKLAVSVGTALYFKMSSHDALAFGLIMCSKGIVELAACSYFYDSNVRVYITLDLY 301
             +   K+A  +   LY K+   +A+A  L++CSK   E+      YD +   YI+   Y
Sbjct: 370 TFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDS---YISQATY 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EDG32.0e-3233.89Cation/H(+) antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=CHX5 PE=2 SV=1[more]
Q9FYC12.7e-3232.10Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1[more]
Q9FFB87.7e-3230.86Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9SIT53.6e-2929.11Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9FYC05.2e-2829.70Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KN981.3e-14699.30Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G450... [more]
A0A5D3BL544.7e-13388.89Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3AUL28.0e-13388.89cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103483015 PE=4 SV=1[more]
A0A6J1DUA72.3e-10368.30cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111024501 PE=... [more]
A0A6J1GPY81.4e-8965.73cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456471 PE=4... [more]
Match NameE-valueIdentityDescription
XP_031741739.19.1e-14799.31cation/H(+) antiporter 4-like [Cucumis sativus][more]
KAE8648452.19.1e-14799.31hypothetical protein Csa_007933 [Cucumis sativus][more]
KAA0066038.19.7e-13388.89cation/H(+) antiporter 4-like [Cucumis melo var. makuwa] >TYJ99857.1 cation/H(+)... [more]
XP_008437675.11.7e-13288.89PREDICTED: cation/H(+) antiporter 4-like [Cucumis melo][more]
KGN51475.21.5e-12893.75hypothetical protein Csa_008150 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G08150.11.4e-3333.89Cation/hydrogen exchanger family protein [more]
AT3G44900.11.9e-3332.10cation/H+ exchanger 4 [more]
AT5G22900.15.5e-3330.86cation/H+ exchanger 3 [more]
AT2G13620.12.5e-3029.11cation/hydrogen exchanger 15 [more]
AT3G44910.13.7e-2929.70cation/H+ exchanger 12 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 1..286
e-value: 1.7E-23
score: 83.0
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 1..307
e-value: 1.5E-37
score: 131.3
NoneNo IPR availablePANTHERPTHR32468:SF17CATION/H(+) ANTIPORTER 4coord: 1..306
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 1..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G15640.1CSPI05G15640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010628 positive regulation of gene expression
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016592 mediator complex
cellular_component GO:0005667 transcription regulator complex
molecular_function GO:0015299 solute:proton antiporter activity