CSPI05G14710 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G14710
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
LocationChr5: 15295117 .. 15297419 (-)
RNA-Seq ExpressionCSPI05G14710
SyntenyCSPI05G14710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAACAAAGTGTGCAAGTTAAAGAGATCATTAGATCGTCTCAAACAATCTCCTAAAGCCTGGTCTGAATGCTTTGAAAAAGCAGTCACAAACTATGAATTCAGTCAAAATCGAGCCGATCATACGATGTTCTATAAACATACAAAAAATGACAAGGTAAGGTTTTGATAGTTTGTTGATATCATTCTTGCAGGCAATGATGAGACAAGATTAACTTTCTTGAAAAGAAAAATTTGATGATTTCCAAATCAAAGAACTAGGAACCTTAAAGTACTTCCTAGGCATGGAGTTTTGTCAAGTGCAAAATTGGAATTCTTGTCAACCAAAGGAGGTATATTCTTGATCTGTTAGAAGAGACAGGTTTACTTGGTTGCCAGGTAGCCGAAACTCCGATTGAGTAGAATCTAAAATTTGAAGCTACAATGAAAATTGAGATAAAGGAAAGAAAAAATGTCAGAGACTTATTGGGAGATATCTTTTTTTCACACAAGTCCTGACATCGCTTTTGCAGTTAGTATGGTAAGTCAGTTCATGCATGCTCTTGGGCTAGCTCACTTTGATGCAGTTTATAGAATCCTAAGACATTTGAAAGGTACTCTAGGAAAACGCATATTGCTTAAAGAGCATGATCATCTCAATGTTGAAGTTTACACTTATGCTGATTGAGCAGGTAGCACGACTACTAGAAGAGCCACGTCTGGTTACTACTCTTTTGTTGGAGGAATTTTGTTACATGGCGAAGTAAAAAACAGTGTGGTTGCAAGTAGTGTAGAAGCAGAATTTAGAGCTTTAGCCCATGGTATTTGTGAAGGTATATGGATAAGAAGACTTTTGGAAAAATTGAGATTCACTCAGACGAGGCTCATGTGCAGTTACGGTGACAACAAGGCAGCAATTTCCATTGCCCACAATCCAGTCCTTCATGACAGGACGAAACATATTGAAGTTGATAAATACTTTATAAAGGAAAAGGTCGATGCAAGAGTAATATACATACCCTACCTTCTGACAACAGAACAAATTGCAGACGTATTAACTAAAGGCCTTCCAATGTGGCAATTCAACAAATTGATTGACAAGCTGGCAACGACTGATATCTTCAAACCTTGAGGGGGAATGTTGATTGTTTCCTTTTCGTATTATATCGGTTGTATTATATTTGCCAAAATTATTTTTTCCTTATTTGTAATGGGTTCTTCTATTTAAGAAAATCCTTCTCTCTTTGTGAAATATACACAAAATTACATTCTTTGGCATCTTGGATGTGTAAATCAACATGTTAACACTTGTGCTCATTTTTTTTCAGGTGAATGAAGAACTAGGAAGCATATTCCCCTTGTTCAAGGAATTTTCGAGCAGTGGCAATGCTTTAGAAAGGGTACTAGCTCTAGAGATCGAGCTTGCTGAAGCTTTGCGGTCAAAAAAGAAACCAAGTATGCATTTTCAGAGGTACATCAACCAGTACAACCATGCCCAACAATTTGATATTTATAGCAGTTTTCTTAGCTTAAAAGCACTATTAGATGCAGAAGTATCTATGTTTCTTAGGTTGTTTGAAGTTGAACTATTCTAATGGTTTCAATAAAAATTTTGGTCTGTGGTCCAGTTCTTTCTTGAAGCAACACAGTGATGAAGAAGCGATATATCGAAGCTTTAGCGACATCAATGAGCTAATAAAAGACATGTTAGATATAAAGGGAAAGTACACAACTGTAGAGACTGAACTGAGAGAGATGCATGATCGTTACTCCCAGTTAAGCCTCCAGTTTGCTGAGGTTGAAGGGGAGAGACAGAAACTCATGATGACTGTCAAGAATGTCCGAGCATCCAAGAAGCTTCTCAACGCCAATAATCGACTCTCATGGTCATCCCGGGGGGAGCATTCTCCTTCATAACTTCTTGGCTTCCTAAGATAAGTCTCTCTTGTCTCTTTCACTAGTTGTTGAAATCGCATTCAGGTCAAATGTGACACCAAAGGCTTCATCTTTGGCTTTCTGCATCACAACACCAAGAAGAATATCGACAGGCGATACTGTCTCCAGGGATCGAGCTGCTACGAGTTTAGAGTCAGCTGCACGATAAAGATGCAACAGTTTTGTGTAAATAAGAAAGCTTCTGCACCTATTCATTCACCAGTTGGTAGAGTATTATCAAGTATAGAATCTTCTTTTCTACTTCAATGTAATTTTTTCTGTACAGTAAATGTAATATCTTCATGGGTAGATGACTTAACTCTCCAAATATTATGAAGAGAAAGCAATGAAACCATGTCATTATACATCTTTGCCCTTCCTCTCTGC

mRNA sequence

ATGATTAACAAAGTGTGCAAGTTAAAGAGATCATTAGATCGTCTCAAACAATCTCCTAAAGCCTGGTCTGAATGCTTTGAAAAAGCAGTCACAAACTATGAATTCAGTCAAAATCGAGCCGATCATACGATGTTCTATAAACATACAAAAAATGACAAGGCATGGAGTTTTGTCAAGTGCAAAATTGGAATTCTTGTCAACCAAAGGAGGTATATTCTTGATCTGTTAGAAGAGACAGGTTTACTTGGTTGCCAGGTAGCCGAAACTCCGATTGATCCTGACATCGCTTTTGCAGTTAGTATGGTAAGTCAGTTCATGCATGCTCTTGGGCTAGCTCACTTTGATGCAGTTTATAGAATCCTAAGACATTTGAAAGGTAGCACGACTACTAGAAGAGCCACGTCTGGTTACTACTCTTTTGTTGGAGGAATTTTGTTACATGGCGAAGTAAAAAACAGTGTGGTTGCAAGTAGTGTAGAAGCAGAATTTAGAGCTTTAGCCCATGGTATTTGTGAAGGTATATGGATAAGAAGACTTTTGGAAAAATTGAGATTCACTCAGACGAGGCTCATGTGCAGTTACGGTGACAACAAGGCAGCAATTTCCATTGCCCACAATCCAGTCCTTCATGACAGGACGAAACATATTGAAGTTGATAAATACTTTATAAAGGAAAAGGTCGATGCAAGAGTGAATGAAGAACTAGGAAGCATATTCCCCTTGTTCAAGGAATTTTCGAGCAGTGGCAATGCTTTAGAAAGGGTACTAGCTCTAGAGATCGAGCTTGCTGAAGCTTTGCGGTCAAAAAAGAAACCAAGTATGCATTTTCAGAGTTCTTTCTTGAAGCAACACAGTGATGAAGAAGCGATATATCGAAGCTTTAGCGACATCAATGAGCTAATAAAAGACATGTTAGATATAAAGGGAAAGTACACAACTGTAGAGACTGAACTGAGAGAGATGCATGATCGTTACTCCCAGTTAAGCCTCCAGTTTGCTGAGGTTGAAGGGGAGAGACAGAAACTCATGATGACTGTCAAGAATGTCCGAGCATCCAAGAAGCTTCTCAACGCCAATAATCGACTCTCATGGTCATCCCGGGGGGAGCATTCTCCTTCATAACTTCTTGGCTTCCTAAGATAAGTCTCTCTTGTCTCTTTCACTAGTTGTTGAAATCGCATTCAGGTCAAATGTGACACCAAAGGCTTCATCTTTGGCTTTCTGCATCACAACACCAAGAAGAATATCGACAGGCGATACTGTCTCCAGGGATCGAGCTGCTACGAGTTTAGAGTCAGCTGCACGATAAAGATGCAACAGTTTTGTGTAAATAAGAAAGCTTCTGCACCTATTCATTCACCAGTTGGTAGAGTATTATCAAGTATAGAATCTTCTTTTCTACTTCAATGTAATTTTTTCTGTACAGTAAATGTAATATCTTCATGGGTAGATGACTTAACTCTCCAAATATTATGAAGAGAAAGCAATGAAACCATGTCATTATACATCTTTGCCCTTCCTCTCTGC

Coding sequence (CDS)

ATGATTAACAAAGTGTGCAAGTTAAAGAGATCATTAGATCGTCTCAAACAATCTCCTAAAGCCTGGTCTGAATGCTTTGAAAAAGCAGTCACAAACTATGAATTCAGTCAAAATCGAGCCGATCATACGATGTTCTATAAACATACAAAAAATGACAAGGCATGGAGTTTTGTCAAGTGCAAAATTGGAATTCTTGTCAACCAAAGGAGGTATATTCTTGATCTGTTAGAAGAGACAGGTTTACTTGGTTGCCAGGTAGCCGAAACTCCGATTGATCCTGACATCGCTTTTGCAGTTAGTATGGTAAGTCAGTTCATGCATGCTCTTGGGCTAGCTCACTTTGATGCAGTTTATAGAATCCTAAGACATTTGAAAGGTAGCACGACTACTAGAAGAGCCACGTCTGGTTACTACTCTTTTGTTGGAGGAATTTTGTTACATGGCGAAGTAAAAAACAGTGTGGTTGCAAGTAGTGTAGAAGCAGAATTTAGAGCTTTAGCCCATGGTATTTGTGAAGGTATATGGATAAGAAGACTTTTGGAAAAATTGAGATTCACTCAGACGAGGCTCATGTGCAGTTACGGTGACAACAAGGCAGCAATTTCCATTGCCCACAATCCAGTCCTTCATGACAGGACGAAACATATTGAAGTTGATAAATACTTTATAAAGGAAAAGGTCGATGCAAGAGTGAATGAAGAACTAGGAAGCATATTCCCCTTGTTCAAGGAATTTTCGAGCAGTGGCAATGCTTTAGAAAGGGTACTAGCTCTAGAGATCGAGCTTGCTGAAGCTTTGCGGTCAAAAAAGAAACCAAGTATGCATTTTCAGAGTTCTTTCTTGAAGCAACACAGTGATGAAGAAGCGATATATCGAAGCTTTAGCGACATCAATGAGCTAATAAAAGACATGTTAGATATAAAGGGAAAGTACACAACTGTAGAGACTGAACTGAGAGAGATGCATGATCGTTACTCCCAGTTAAGCCTCCAGTTTGCTGAGGTTGAAGGGGAGAGACAGAAACTCATGATGACTGTCAAGAATGTCCGAGCATCCAAGAAGCTTCTCAACGCCAATAATCGACTCTCATGGTCATCCCGGGGGGAGCATTCTCCTTCATAA

Protein sequence

MINKVCKLKRSLDRLKQSPKAWSECFEKAVTNYEFSQNRADHTMFYKHTKNDKAWSFVKCKIGILVNQRRYILDLLEETGLLGCQVAETPIDPDIAFAVSMVSQFMHALGLAHFDAVYRILRHLKGSTTTRRATSGYYSFVGGILLHGEVKNSVVASSVEAEFRALAHGICEGIWIRRLLEKLRFTQTRLMCSYGDNKAAISIAHNPVLHDRTKHIEVDKYFIKEKVDARVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEAIYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNVRASKKLLNANNRLSWSSRGEHSPS*
Homology
BLAST of CSPI05G14710 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-19
Identity = 88/331 (26.59%), Postives = 131/331 (39.58%), Query Frame = 0

Query: 3    NKVCKLKRSLDRLKQSPKAWSECFEKAVTNYEFSQNRADHTMFYKHTKNDKAWSFV---- 62
            N VCKL+++L  LKQ+P+AW       +    F  + +D ++F         +  V    
Sbjct: 1094 NYVCKLRKALYGLKQAPRAWYVELRNYLLTIGFVNSVSDTSLFVLQRGKSIVYMLVYVDD 1153

Query: 63   ---------------------------------------KCKIGILVNQRRYILDLLEET 122
                                                   +   G+ ++QRRYILDLL  T
Sbjct: 1154 ILITGNDPTLLHNTLDNLSQRFSVKDHEELHYFLGIEAKRVPTGLHLSQRRYILDLLART 1213

Query: 123  GLLGCQVAETPI---------------------------------DPDIAFAVSMVSQFM 182
             ++  +   TP+                                  PDI++AV+ +SQFM
Sbjct: 1214 NMITAKPVTTPMAPSPKLSLYSGTKLTDPTEYRGIVGSLQYLAFTRPDISYAVNRLSQFM 1273

Query: 183  HALGLAHFDAVYRILRHL-----------KGSTTTRRA---------------TSGYYSF 230
            H     H  A+ RILR+L           KG+T +  A               T+GY  +
Sbjct: 1274 HMPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLSLHAYSDADWAGDKDDYVSTNGYIVY 1333

BLAST of CSPI05G14710 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-16
Identity = 83/329 (25.23%), Postives = 134/329 (40.73%), Query Frame = 0

Query: 5    VCKLKRSLDRLKQSPKAWSECFEKAVTNYEFSQNRADHTMF------------------- 64
            VC+L++++  LKQ+P+AW       +    F  + +D ++F                   
Sbjct: 1079 VCRLRKAIYGLKQAPRAWYVELRTYLLTVGFVNSISDTSLFVLQRGRSIIYMLVYVDDIL 1138

Query: 65   --------YKHTKNDKAWSF----------------VKCKIGILVNQRRYILDLLEETGL 124
                     KHT +  +  F                 +   G+ ++QRRY LDLL  T +
Sbjct: 1139 ITGNDTVLLKHTLDALSQRFSVKEHEDLHYFLGIEAKRVPQGLHLSQRRYTLDLLARTNM 1198

Query: 125  LGCQVAETPI---------------------------------DPDIAFAVSMVSQFMHA 184
            L  +   TP+                                  PD+++AV+ +SQ+MH 
Sbjct: 1199 LTAKPVATPMATSPKLTLHSGTKLPDPTEYRGIVGSLQYLAFTRPDLSYAVNRLSQYMHM 1258

Query: 185  LGLAHFDAVYRILRHL-----------KGSTTTRRA---------------TSGYYSFVG 230
                H++A+ R+LR+L           KG+T +  A               T+GY  ++G
Sbjct: 1259 PTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSDADWAGDTDDYVSTNGYIVYLG 1318

BLAST of CSPI05G14710 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 4.1e-15
Identity = 76/339 (22.42%), Postives = 131/339 (38.64%), Query Frame = 0

Query: 3    NKVCKLKRSLDRLKQSPKAWSECFEKAVTNYEFSQNRADHTMF----------------- 62
            + VCKL +++  LKQ+ + W E FE+A+   EF  +  D  ++                 
Sbjct: 1029 DNVCKLNKAIYGLKQAARCWFEVFEQALKECEFVNSSVDRCIYILDKGNINENIYVLLYV 1088

Query: 63   -----------------------YKHTKNDKAWSFVKCKI-----GILVNQRRYILDLLE 122
                                   ++ T  ++   F+  +I      I ++Q  Y+  +L 
Sbjct: 1089 DDVVIATGDMTRMNNFKRYLMEKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILS 1148

Query: 123  ETGLLGCQVAETPID---------------------------------PDIAFAVSMVSQ 182
            +  +  C    TP+                                  PD+  AV+++S+
Sbjct: 1149 KFNMENCNAVSTPLPSKINYELLNSDEDCNTPCRSLIGCLMYIMLCTRPDLTTAVNILSR 1208

Query: 183  FMHALGLAHFDAVYRILRHLK----------------------------GSTTTRRATSG 232
            +        +  + R+LR+LK                            GS   R++T+G
Sbjct: 1209 YSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTG 1268

BLAST of CSPI05G14710 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 9.0e-15
Identity = 55/163 (33.74%), Postives = 89/163 (54.60%), Query Frame = 0

Query: 93   PDIAFAVSMVSQFMHALGLAHFDAVYRILRHLKGST------------------------ 152
            PDIA AV +VS+F+   G  H++AV  ILR+L+G+T                        
Sbjct: 1127 PDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILKGYTDADMAGD 1186

Query: 153  -TTRRATSGY-YSFVGG-ILLHGEVKNSVVASSVEAEFRALAHGICEGIWIRRLLEKLRF 212
               R++++GY ++F GG I    +++  V  S+ EAE+ A      E IW++R L++L  
Sbjct: 1187 IDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGL 1246

Query: 213  TQTRLMCSYGDNKAAISIAHNPVLHDRTKHIEVDKYFIKEKVD 229
             Q   +  Y D+++AI ++ N + H RTKHI+V  ++I+E VD
Sbjct: 1247 HQKEYVV-YCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVD 1288

BLAST of CSPI05G14710 vs. ExPASy Swiss-Prot
Match: P92519 (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 7.4e-09
Identity = 47/173 (27.17%), Postives = 74/173 (42.77%), Query Frame = 0

Query: 63  GILVNQRRYILDLLEETGLLGCQVAETPID------------------------------ 122
           G+ ++Q +Y   +L   G+L C+   TP+                               
Sbjct: 53  GLFLSQTKYAEQILNNAGMLDCKPMSTPLPLKLNSSVSTAKYPDPSDFRSIVGALQYLTL 112

Query: 123 --PDIAFAVSMVSQFMHALGLAHFDAVYRILRHLKGS----------------------- 176
             PDI++AV++V Q MH   LA FD + R+LR++KG+                       
Sbjct: 113 TRPDISYAVNIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDW 172

BLAST of CSPI05G14710 vs. ExPASy TrEMBL
Match: A0A0A0KQA1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409665 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 2.3e-69
Identity = 142/144 (98.61%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 230 RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
           +VNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 141 KVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 200

Query: 290 IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
           IYRSFSDINELIKDMLD+KGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV
Sbjct: 201 IYRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 260

Query: 350 RASKKLLNANNRLSWSSRGEHSPS 374
           RASKKLLNANNRLSWSSRGEHSPS
Sbjct: 261 RASKKLLNANNRLSWSSRGEHSPS 284

BLAST of CSPI05G14710 vs. ExPASy TrEMBL
Match: A0A5D3DSB6 (Myosin-2 heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G002260 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 4.7e-67
Identity = 139/147 (94.56%), Postives = 143/147 (97.28%), Query Frame = 0

Query: 227 VDARVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD 286
           V  RVNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD
Sbjct: 19  VFVRVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD 78

Query: 287 EEAIYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTV 346
           EEAI+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTV
Sbjct: 79  EEAIFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTV 138

Query: 347 KNVRASKKLLNANNRLSWSSRGEHSPS 374
           KNVRASKKLLNANNR SWS RGEHSPS
Sbjct: 139 KNVRASKKLLNANNRPSWSYRGEHSPS 165

BLAST of CSPI05G14710 vs. ExPASy TrEMBL
Match: A0A1S3CD41 (myosin-2 heavy chain OS=Cucumis melo OX=3656 GN=LOC103499300 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 137/144 (95.14%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1746 KVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1805

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTVKNV
Sbjct: 1806 IFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTVKNV 1865

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNR SWS RGEHSPS
Sbjct: 1866 RASKKLLNANNRPSWSYRGEHSPS 1889

BLAST of CSPI05G14710 vs. ExPASy TrEMBL
Match: A0A5A7V2E5 (Myosin-2 heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G001580 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 137/144 (95.14%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1746 KVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1805

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTVKNV
Sbjct: 1806 IFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTVKNV 1865

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNR SWS RGEHSPS
Sbjct: 1866 RASKKLLNANNRPSWSYRGEHSPS 1889

BLAST of CSPI05G14710 vs. ExPASy TrEMBL
Match: A0A6J1FEV0 (centrosomal protein of 290 kDa-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444849 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 2.6e-65
Identity = 134/144 (93.06%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELGSIFPLFKEFSS GN+LERVLALEIELAEAL++KKKPS HFQSSFLKQHSDEEA
Sbjct: 1731 KVNEELGSIFPLFKEFSSRGNSLERVLALEIELAEALQAKKKPSTHFQSSFLKQHSDEEA 1790

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV
Sbjct: 1791 IFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 1850

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RAS+KLLNANNR SWSSRGEHSPS
Sbjct: 1851 RASRKLLNANNRPSWSSRGEHSPS 1874

BLAST of CSPI05G14710 vs. NCBI nr
Match: XP_011655223.1 (myosin heavy chain, skeletal muscle isoform X2 [Cucumis sativus])

HSP 1 Score: 272.7 bits (696), Expect = 4.7e-69
Identity = 142/144 (98.61%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1735 KVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1794

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            IYRSFSDINELIKDMLD+KGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV
Sbjct: 1795 IYRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 1854

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNRLSWSSRGEHSPS
Sbjct: 1855 RASKKLLNANNRLSWSSRGEHSPS 1878

BLAST of CSPI05G14710 vs. NCBI nr
Match: XP_004140370.1 (myosin heavy chain, skeletal muscle isoform X1 [Cucumis sativus] >XP_011655222.1 myosin heavy chain, skeletal muscle isoform X1 [Cucumis sativus] >XP_031741976.1 myosin heavy chain, skeletal muscle isoform X1 [Cucumis sativus] >XP_031741977.1 myosin heavy chain, skeletal muscle isoform X1 [Cucumis sativus])

HSP 1 Score: 272.7 bits (696), Expect = 4.7e-69
Identity = 142/144 (98.61%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1742 KVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1801

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            IYRSFSDINELIKDMLD+KGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV
Sbjct: 1802 IYRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 1861

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNRLSWSSRGEHSPS
Sbjct: 1862 RASKKLLNANNRLSWSSRGEHSPS 1885

BLAST of CSPI05G14710 vs. NCBI nr
Match: TYK26596.1 (myosin-2 heavy chain [Cucumis melo var. makuwa])

HSP 1 Score: 265.0 bits (676), Expect = 9.8e-67
Identity = 139/147 (94.56%), Postives = 143/147 (97.28%), Query Frame = 0

Query: 227 VDARVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD 286
           V  RVNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD
Sbjct: 19  VFVRVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSD 78

Query: 287 EEAIYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTV 346
           EEAI+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTV
Sbjct: 79  EEAIFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTV 138

Query: 347 KNVRASKKLLNANNRLSWSSRGEHSPS 374
           KNVRASKKLLNANNR SWS RGEHSPS
Sbjct: 139 KNVRASKKLLNANNRPSWSYRGEHSPS 165

BLAST of CSPI05G14710 vs. NCBI nr
Match: KAA0062382.1 (myosin-2 heavy chain [Cucumis melo var. makuwa])

HSP 1 Score: 263.5 bits (672), Expect = 2.8e-66
Identity = 137/144 (95.14%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1746 KVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1805

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTVKNV
Sbjct: 1806 IFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTVKNV 1865

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNR SWS RGEHSPS
Sbjct: 1866 RASKKLLNANNRPSWSYRGEHSPS 1889

BLAST of CSPI05G14710 vs. NCBI nr
Match: XP_008460500.1 (PREDICTED: myosin-2 heavy chain [Cucumis melo] >XP_008460502.1 PREDICTED: myosin-2 heavy chain [Cucumis melo] >XP_008460503.1 PREDICTED: myosin-2 heavy chain [Cucumis melo])

HSP 1 Score: 263.5 bits (672), Expect = 2.8e-66
Identity = 137/144 (95.14%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +VNEELG+IFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA
Sbjct: 1746 KVNEELGNIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 1805

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I+RSFSDINELIKDMLD+KGKYTTVETELREMHDRYS+LSLQFAEVEGERQKLMMTVKNV
Sbjct: 1806 IFRSFSDINELIKDMLDLKGKYTTVETELREMHDRYSKLSLQFAEVEGERQKLMMTVKNV 1865

Query: 350  RASKKLLNANNRLSWSSRGEHSPS 374
            RASKKLLNANNR SWS RGEHSPS
Sbjct: 1866 RASKKLLNANNRPSWSYRGEHSPS 1889

BLAST of CSPI05G14710 vs. TAIR 10
Match: AT1G22060.1 (LOCATED IN: vacuole; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: FBD, F-box and Leucine Rich Repeat domains containing protein (TAIR:AT1G22000.1); Has 84739 Blast hits to 38714 proteins in 2257 species: Archae - 1436; Bacteria - 11314; Metazoa - 40747; Fungi - 7706; Plants - 4675; Viruses - 308; Other Eukaryotes - 18553 (source: NCBI BLink). )

HSP 1 Score: 190.3 bits (482), Expect = 2.9e-48
Identity = 103/141 (73.05%), Postives = 121/141 (85.82%), Query Frame = 0

Query: 230  RVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSKKKPSMHFQSSFLKQHSDEEA 289
            +  EEL SIFPL +E  S GNALERVLALEIELAEALR KKK + HFQSSFLKQH+D+EA
Sbjct: 1860 QAKEELQSIFPLSQENFSCGNALERVLALEIELAEALRGKKKSTTHFQSSFLKQHTDDEA 1919

Query: 290  IYRSFSDINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNV 349
            I++SF DIN LI++MLD KG+Y+++ETELREMHDRYSQLSL+FAEVEGERQKLMMT+KNV
Sbjct: 1920 IFQSFRDINNLIEEMLDTKGRYSSMETELREMHDRYSQLSLKFAEVEGERQKLMMTLKNV 1979

Query: 350  RASKKLLNANNRLSWSSRGEH 371
            RASKK +   NR S ++ GEH
Sbjct: 1980 RASKKAM-LLNRSSSATLGEH 1999

BLAST of CSPI05G14710 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 100/328 (30.49%), Postives = 144/328 (43.90%), Query Frame = 0

Query: 3   NKVCKLKRSLDRLKQSPKAWSECFEKAVTNYEFSQNRADHTMFYKHT------------- 62
           N VC LK+S+  LKQ+ + W   F   +  + F Q+ +DHT F K T             
Sbjct: 227 NAVCYLKKSIYGLKQASRQWFLKFSVTLIGFGFVQSHSDHTYFLKITATLFLCVLVYVDD 286

Query: 63  -----KNDKAWSFVKCKI-------------------------GILVNQRRYILDLLEET 122
                 ND A   +K ++                         GI + QR+Y LDLL+ET
Sbjct: 287 IIICSNNDAAVDELKSQLKSCFKLRDLGPLKYFLGLEIARSAAGINICQRKYALDLLDET 346

Query: 123 GLLGCQVAETPIDP---------------------------------DIAFAVSMVSQFM 182
           GLLGC+ +  P+DP                                 DI+FAV+ +SQF 
Sbjct: 347 GLLGCKPSSVPMDPSVTFSAHSGGDFVDAKAYRRLIGRLMYLQITRLDISFAVNKLSQFS 406

Query: 183 HALGLAHFDAVYRILRHLKGST--------------------------TTRRATSGYYSF 227
            A  LAH  AV +IL ++KG+                            TRR+T+GY  F
Sbjct: 407 EAPRLAHQQAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASFQSCKDTRRSTNGYCMF 466

BLAST of CSPI05G14710 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 5.3e-10
Identity = 47/173 (27.17%), Postives = 74/173 (42.77%), Query Frame = 0

Query: 63  GILVNQRRYILDLLEETGLLGCQVAETPID------------------------------ 122
           G+ ++Q +Y   +L   G+L C+   TP+                               
Sbjct: 53  GLFLSQTKYAEQILNNAGMLDCKPMSTPLPLKLNSSVSTAKYPDPSDFRSIVGALQYLTL 112

Query: 123 --PDIAFAVSMVSQFMHALGLAHFDAVYRILRHLKGS----------------------- 176
             PDI++AV++V Q MH   LA FD + R+LR++KG+                       
Sbjct: 113 TRPDISYAVNIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDW 172

BLAST of CSPI05G14710 vs. TAIR 10
Match: AT5G52280.1 (Myosin heavy chain-related protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.8e-09
Identity = 28/59 (47.46%), Postives = 47/59 (79.66%), Query Frame = 0

Query: 296 DINELIKDMLDIKGKYTTVETELREMHDRYSQLSLQFAEVEGERQKLMMTVKNVRASKK 355
           ++++L  ++   K K +++E EL+EM +RYS++SL+FAEVEGERQ+L+M V+N++  KK
Sbjct: 794 NLSKLSDELAYCKNKNSSMERELKEMEERYSEISLRFAEVEGERQQLVMAVRNLKNGKK 852

BLAST of CSPI05G14710 vs. TAIR 10
Match: AT5G41140.1 (Myosin heavy chain-related protein )

HSP 1 Score: 59.3 bits (142), Expect = 7.6e-09
Identity = 47/145 (32.41%), Postives = 82/145 (56.55%), Query Frame = 0

Query: 226 KVDARVNEELGSIFPLFKEFSSSGNALERVLALEIELAEALRSK--KKPSMHFQSSFLKQ 285
           K + R NE+   I  L  +     NALE    + IE  + L+++  +  +   + S   Q
Sbjct: 840 KTEQRSNED--RIKQLEGQIKLKENALEASSKIFIEKEKDLKNRIEELQTKLNEVSQNSQ 899

Query: 286 HSDE-----EAIYRSFSDI---------NELIKDMLDIKGKYTTVETELREMHDRYSQLS 345
            +DE     EAI   ++++          +L+ ++  ++ +   +ETEL+EM +RYS++S
Sbjct: 900 ETDETLQGPEAIAMQYTEVLPLSKSDNLQDLVNEVASLREQNGLMETELKEMQERYSEIS 959

Query: 346 LQFAEVEGERQKLMMTVKNVRASKK 355
           L+FAEVEGERQ+L+MTV+ ++ +KK
Sbjct: 960 LRFAEVEGERQQLVMTVRYLKNAKK 982

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94HW21.6e-1926.59Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT941.3e-1625.23Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P041464.1e-1522.42Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
P109789.0e-1533.74Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P925197.4e-0927.17Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KQA12.3e-6998.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409665 PE=4 SV=1[more]
A0A5D3DSB64.7e-6794.56Myosin-2 heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313... [more]
A0A1S3CD411.4e-6695.14myosin-2 heavy chain OS=Cucumis melo OX=3656 GN=LOC103499300 PE=4 SV=1[more]
A0A5A7V2E51.4e-6695.14Myosin-2 heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154... [more]
A0A6J1FEV02.6e-6593.06centrosomal protein of 290 kDa-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
XP_011655223.14.7e-6998.61myosin heavy chain, skeletal muscle isoform X2 [Cucumis sativus][more]
XP_004140370.14.7e-6998.61myosin heavy chain, skeletal muscle isoform X1 [Cucumis sativus] >XP_011655222.1... [more]
TYK26596.19.8e-6794.56myosin-2 heavy chain [Cucumis melo var. makuwa][more]
KAA0062382.12.8e-6695.14myosin-2 heavy chain [Cucumis melo var. makuwa][more]
XP_008460500.12.8e-6695.14PREDICTED: myosin-2 heavy chain [Cucumis melo] >XP_008460502.1 PREDICTED: myosin... [more]
Match NameE-valueIdentityDescription
AT1G22060.12.9e-4873.05LOCATED IN: vacuole; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 gro... [more]
AT4G23160.11.2e-3030.49cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
ATMG00810.15.3e-1027.17DNA/RNA polymerases superfamily protein [more]
AT5G52280.15.8e-0947.46Myosin heavy chain-related protein [more]
AT5G41140.17.6e-0932.41Myosin heavy chain-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34452MYOSIN HEAVY CHAIN-RELATED PROTEINcoord: 230..365
NoneNo IPR availablePANTHERPTHR34452:SF1SPORULATION-SPECIFIC PROTEINcoord: 230..365
NoneNo IPR availableCDDcd09272RNase_HI_RT_Ty1coord: 126..231
e-value: 5.7787E-37
score: 128.74

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G14710.1CSPI05G14710.1mRNA