CSPI05G14530 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G14530
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptiontRNA modification GTPase MnmE
LocationChr5: 14997162 .. 15004137 (-)
RNA-Seq ExpressionCSPI05G14530
SyntenyCSPI05G14530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAATGTAATACTTTTTTTTAAAAGGTTTTTCTCATATGCAATTTCCCTTGTATATATTTTGCTCTTATCTTTCAAAAACACGCCAGCCCCGGCAATCCTCGCCTTTGGCCTAGTTTCGTGGCATGGCTCTGCTCCCAGGGTTCCGCCATTTCATCGCCCATTTTTACAGAACCACACCACCAATGGCTTTTCTCTTTACCCATTTCTCTACCCCTATTTCTAGACCATCCTCTATATATTCCATATCCAAAACCTCTAACCATGTTCTCTCAAAATCCCTGATCAAGTCTCACTCTACTGGGAAAGGTCACCTCCTTATTAATTTGCTCTTATTTTTACTTCAACTTTATTGGAATTCTCCATTTTCTCATTCTTCTTCTTCTTCCTGCAGAGAATACGTTCGTTCTTGCTCCAGATGAGCGCTTGGGGGATTCGCATGCCGGGACTGAGCGTGAACAAATTGACAATTCAAGCACAATTGCTGCCATTGTGACATCCATTGGGGGTCCCCCTGCTGCTGTGGGGATTGTGCGTTTATCGGGTCCTCGTGCTGTAAATATCGTTGGGACTTTATTTTTCCCTGCCGCCAAAAAGAAGGGGAAAAATTTGAGCCTGCACCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGATGAAGTACAGTAATGTTTACCTTGTCATCTTTGGAAAATTCTTTCAATTCTGTGTCGTTATAGGCTTTTGTTAGTTTGGGATTAATTTGAGCAATGGTTTTGCTATTTTGTGAAGGTTTTGACAGTGCCAATGTTGGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGTCGTGTGCTAAAAGCTTGTTTAGAAGCTGGAGCTCGGCTTGCAGAACCAGGTTTATTTTCTGAATTGGGATTGCTTTCTCATTAAATTTATGGACTATACACAAATCCCCTGCTCATTTGTTTACATTAAGTGTTTGCTTGCATCGGAATTAAACCAGTGCTAATCCATGGAAAAAAGAAGTGGGGAAAATTGGTTTAGGCTAAGAATTTATGCTTTTCATTATGATATCGTTACCAAATCTACACTTATGATTGCCTAGGGGAAAGAAACGTTTATAATTAAGATTTTGTATGTATAGGTGAGTTTACTCTTCGTGCCTTCTTAAATGGACGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCCGATGCTGCACTGGCTGGAATTCAGGTTGGGACCTTTTATTTGACTGATAATGTTGTCTGAACTGGTGTTTCCATCTAATTTCAGCTACCGAGTTACTGCAACTTGGTGTGGAAGCTAACAATGACTTCTAGTTTTTTTGAAACGATAAAACGATACGGATCTCTTTGTTAATAGTGACCGAGCTCAAAGTACAAGAGGGTTATACATGAGTGGTAAAAAATACTAGGGATCAGCAGTTCCGTTCAGACATCTCAACTAGGTTGTCACCTCCTTAGCGACCTCATCATATCAGAAGAACAAAAGCAAAAGAAAAAAGAACTCAATACTAAGCCATGATAGTAGATCTTCACAAAGCCATCATATACAAAGTTCAATACTAAGCCATCATATCCAATGACTTCTAGTTTACTGAAGCATATCATATTTTGATTGGCAGGGTGGCTTCTCTTCCTTAGTCAAATCGCTAAGAACACAATGTATCGAATTACTCACTGAAATTGAGGCTCGTCTAGACTTTGATGATGAGATGCCACCGTTGGATTTGAACATTGTAATGGAAAAAGTGCATGCCATGTCACAGGAAGTAGAGACTGCTCTTGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGGTAAATTTTAAGCACTGTCTAAATCAGTTTGTTTCCTTGCACAAAAGATGCATGGATTTGTTAATACCATTTTCCATCTCCTTAAAACTTGCAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAGTAAGCTACATGACATTCTCTTATCTTCTTTGAACTTGACCTCCTTTAGATAATTTGTTCTATCTATGAGTCTGACTCATAGTCTTACTTAAAAATGGGAAGTAAAAAAGTTTAATATCATCCCTCTGTATTACTGAACTAAATACAAGGTCTTCTTATATAGAGAGACCAACAACAAATAAGGAAATTTTTTTGACATAAATAAAGAACAAAATAATATTACAAGAGAAAAGGAAACTCAACACTTCCCCTCAAGCTTGTTTGTAGATATCATCCATAGCCAACATGCTAAGACATTTATCAAATTGTTTTTTTCGAAAGACCTTTAGTTAGAATGTTCGCTGCTTGTTCTTTTGTAGGAAGATAATATATGTACATTATACTTGGATGAACCTTCTCCTTTATGAAATGCTTGCCAACTTCACTGTGCTTCGTCCTATCATGTAGAAGTGGATTATGAGTTATAGCAATGACAACATTGTCATCACAATAAACTTGTATAGGTGTCTTTGGAGACACTTTCATTTCTTCAAGGATTTTGTTAAGCCATATGCCTTCACAAATTACATGGGCCAAGGCTCTAAATTCTGCTTCAGCACTACTCCTAGCCGCTACATTTTGTTTTTTACTCCACCATGTACCTTAATTTTCTCCAACAAATAAAAGATCTTTTATCAGTAGTACTTCCACCCCTGTGTTAGTAGACAAAGAACAACACAGTCGGCCTGAGAACCAAGCCAGGAAAAATAGTTCTAAAGCAGCAAGCAGGAGTGCAGGAATGAACTGGGAAAAACCAAATCAGGTGAAAACCAAACCGCACCCAATCTAAACCAGCCAAGTTCAATCACATAACGATATACAAACCAGAGATCCAAAAAAATTTATGGTGGAATTCCAGCTGGGTTTTGGCAGCGATTTGGGCAAGGTGTCTGAAACGAGAAAACTCCGGCTGTAACTACGTTATTTGTGAGGTTCTGTGAACTCCCATTTCTACTGTGAAAAAGGTGGCAAACTCGAGCAGTGGCAATGGAGACTGCAACAGCATGTGATTCTGGCGAGTTTAGGTTGAAAAATCCCACTCAATTGTGTGGGTATGGAAGTTTGGAGAAGACAGTCGTAACTCACCCCCTGGCCACTGCAAATAGGAAAGTTTGGTTTGGAAGTAGGATGTGCCACTGATTTCGACATCTAGGGTTCCGACGGTGGCTATGGTTGTGTAGTGGTGGTTAAAATTACCAACATACAGCAATAAATGCAAGAAAACGCAGCTGCTGGTGGTGTCCAGGAAGTTGTGGTCCTGTGGTCGGCTGCAGCGACTGGAGAAGGTCTTCTTATATAGAGAAATATCAGCAACCAATAAGGAAAAGAAATTACAGGCATAAATAAAGAACGAAATAATATAAGAGAAAAGGACATTTAACAATCTTCATGTCAGTTTTTGCTTGTTCCTTGTTGTTTCCATTGAGTCAACGTCTATCCGCTTACTCTTAATATCACCTAGTGCTTTGTCACTGTAAGAGGGAGAGGGATACTCTTGCATGACTTCCAGATTTGGTGTTATGTGAATTGAAATTTGAGAAAATCTTGGTACTGTATTACTACTTTCACACTAGTAATGGTTTTTCTGAAAAGGGTGGTGAAACATCTGAATATTCTTCCTTCACATGGAAAGGATTGATTTGAATTTTGAAGGACTGTTGGTTGAGAAGCACAGTGAGGATTTTATAGTAACTCCTGCAGAGGAAGGGGCTCTATTCAACATTTCTTGAAATATGAACATTGTGTTGTGGCTCTTAGCTCTTCATGGTCAAGTTTGTTGCATTGCAAATATGGGGACAGTCAAACCTATTGTGCAAAATTCTGGGATGGTGGAGGGGACCGGGCATGAACACATGAGGTAGTAAAGGAGTTGGTAATCTACCTGATGCTATATTGGATTTAACTTGCTAATGGCTTTTTCCATTATTCTGTGCAGTGTGCCTATGTTGACATGGAGTTGCAGAAAAAGATGACAATGAGGAGTTTCACAAAAGTTTGTAGGAGCTCATTAAATTTGGGAATCTGGTGTCTTATGTTTTAGTCCTTACAATGAACATGGGGTTTAGCGGCTAGATCTCAAAGAAGCCTTGCTAAAGACACAGTTTGGAAGCTGGTTGAGTAGGCATATGAAATTGAATTTGGTTGATAGGTTGTTTGTAGAAGTCCGTCTTGAGCATCAGAGAGATTTTTTATGCCTGTTGGTTGTGGTATACATTTTTAGCCACTTCTCTGCAGTCTGCACTTCCCTCCAAAATTAAAAAAAATTCATCGTTGCTGCTTTGTGGCCATATGTTTAAGAAGCAATCAAGACATTTTCAGGAAAATAAGTTCTTTGACTAGATGCTACTAAATCTTTTATGAGTAAGTGTGGTTAAAGAGAAAGTAGAGAACTTTTTAAGGAGTGTAAAGTGTTAGAATAAATTAGGGTTTACGATCAAATTAATATTTTCCTTTCTAGTATTTTCTTTTTCTGTTATTCTCCTTGTTTGTATTATTGGGAGTCTATTTGTTCAGTAATTTTCCATTATGATTATTCAAGTTATTATTCAATACTATTATAAATATTTAACATGGTATCATAGCTTTAGGGTTTCTTTCATATTAATCCACTTCTGCCACTTTGATTCTGTCTTCGTGGGCGTCACCGTCGGTGGAGACAGCAAAATCTGCTTTAATGCAATTCAGCGCTGCCAGTTTCGTCTTCATTTTTCTAGTTAGTTTGATCTACTGTTTAGTTGATTTTGTTCTTTGTGTTTTGGTCTGTTCCATTTTCAGATCAGCAGTTCGTTGGTTTTGTTTTAGATCTCTCTATGAAAATCTCTGTTTTCAGTGCAAAAGATCTCTGTGTTTCAGTTCTTGGGTTGTGTCTCCAATCTAGAAGTTCATGGGTTTTACTTTACGTATATCTCTATTTTAGCTTAGAAGTTCGTGGGTAGACCTTTACAATGTCACTGTTTCTAGTCTAGAAGTTCGTGAATTCTTGTTTCCTACCTACAAGTTCTTTGTGTTCTTGTTTCCTACCTCTGTGTCCAACCCAGACCTGCTGTGATTTGATTCAGGAGTTTGCTTCCATTTCTATCTTTGAGTCACTTCAGTCCATCGTACACAAACTCAGTTGTTTCTACCACCTTGAGTTCTTTCAACTATTCCTCCGCTATGCTAGACAATAGGAAAGTCAAACCTATATATAACTAAGAAACATGCAGCATTTCTGCTTTTGTGAGGGTGATTACCCTCTGTCGTATTTTCATTTATTCTATGAATTGTTTGTGTGTTAGTCTCACTAAAAAGAAATTGTGAATTTGTTAAAAAAAATTTAACCTCACAACAGAAATTTCTGTTTGATATTAGGTGGTTTTCTTTAGCTACCTTTTCTTTTACTCTTAATCACATTATTCTATTGTCACATTTATTTTTTAGCGTGCAGAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAATGTTACAGTTTCTGGTATTCCTGTGACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTATGATTTACGAATTTCCTAATGTTTAGTGTTTTAATTCAGCATTTCACATGATGCCCAATTATTCCCTTTGAGCTAGGTTGATTTGCATTTAGTAATTGTAACTTATCCTAGGCCTGATCTACCATCAGAACTATACTGATTGATTATAATAAGATTTTGTTATGGCTTTTGTTATTTCCCCTTTCAGTCTCGAACTCGGGTCAATTTTAATTTGGAAGAAATTATTTTATTGGCATGTTTGATTTTTCTTGAGTTGCAAATTGACACCCGTTCTCCTACTCTGTTCTACAGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCAGATGTCATTATTATGGCAATAAGTGCCCTCGATGGATGGACTGCCGAAGATACCATACTTCTCAATAGGATACTTTCAAAAAAGGTTTAGCAAAATTCGACCACCTGTTTCCTGCAATGAATTCTTCTTTAATGAATCACTGCTCAAAATCAAAGCTCCACCACCATAAAGAATATTAAAAATGATGTTTATTTCAATTTCTTTTAAACGCTTTGAATATGCAGAAATCAGATGAATCATGCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTCCAAAGATGGATGCAATGAGTATAAATCGTGATTCTTTCAGTAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACGGTAAACCAGGTATAACTAACCTATTTCTCTCTTCCCCGTACCAGATAAGAATCCGGTTCTTTGATGGTAAAACTTGATATAACATCATTTCTTGTCTTAATAAATAGAAATAATAATGAGCAATGTAGAACTCATGATTTAGTGTTGTTTTGGTTACCTTCAGAGACAATGCGTGCAGCTTCTTAGAACCAAGGAGGCATTTACAAGATTGAAATCATCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGATTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAGATAGTATCATCAATGTATGTTTGATCTCATATTATTTACATTTCTAGCCATGGCTCCCACAAGTCTTTTTCGGGGGATTACGCCATTTTGGTCACTCAACTTTTGGAGGTTTTTTCTTTGGGCATTTTCAAATATAGTCAAATGAACTAAAATATTACAAA

mRNA sequence

GACAATGTAATACTTTTTTTTAAAAGGTTTTTCTCATATGCAATTTCCCTTGTATATATTTTGCTCTTATCTTTCAAAAACACGCCAGCCCCGGCAATCCTCGCCTTTGGCCTAGTTTCGTGGCATGGCTCTGCTCCCAGGGTTCCGCCATTTCATCGCCCATTTTTACAGAACCACACCACCAATGGCTTTTCTCTTTACCCATTTCTCTACCCCTATTTCTAGACCATCCTCTATATATTCCATATCCAAAACCTCTAACCATGTTCTCTCAAAATCCCTGATCAAGTCTCACTCTACTGGGAAAGAGAATACGTTCGTTCTTGCTCCAGATGAGCGCTTGGGGGATTCGCATGCCGGGACTGAGCGTGAACAAATTGACAATTCAAGCACAATTGCTGCCATTGTGACATCCATTGGGGGTCCCCCTGCTGCTGTGGGGATTGTGCGTTTATCGGGTCCTCGTGCTGTAAATATCGTTGGGACTTTATTTTTCCCTGCCGCCAAAAAGAAGGGGAAAAATTTGAGCCTGCACCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGATGAAGTTTTGACAGTGCCAATGTTGGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGTCGTGTGCTAAAAGCTTGTTTAGAAGCTGGAGCTCGGCTTGCAGAACCAGGTGAGTTTACTCTTCGTGCCTTCTTAAATGGACGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCCGATGCTGCACTGGCTGGAATTCAGGGTGGCTTCTCTTCCTTAGTCAAATCGCTAAGAACACAATGTATCGAATTACTCACTGAAATTGAGGCTCGTCTAGACTTTGATGATGAGATGCCACCGTTGGATTTGAACATTGTAATGGAAAAAGTGCATGCCATGTCACAGGAAGTAGAGACTGCTCTTGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAATGTTACAGTTTCTGGTATTCCTGTGACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCAGATGTCATTATTATGGCAATAAGTGCCCTCGATGGATGGACTGCCGAAGATACCATACTTCTCAATAGGATACTTTCAAAAAAGAAATCAGATGAATCATGCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTCCAAAGATGGATGCAATGAGTATAAATCGTGATTCTTTCAGTAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACGGTAAACCAGAGACAATGCGTGCAGCTTCTTAGAACCAAGGAGGCATTTACAAGATTGAAATCATCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGATTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAGATAGTATCATCAATGTATGTTTGATCTCATATTATTTACATTTCTAGCCATGGCTCCCACAAGTCTTTTTCGGGGGATTACGCCATTTTGGTCACTCAACTTTTGGAGGTTTTTTCTTTGGGCATTTTCAAATATAGTCAAATGAACTAAAATATTACAAA

Coding sequence (CDS)

ATGGCTCTGCTCCCAGGGTTCCGCCATTTCATCGCCCATTTTTACAGAACCACACCACCAATGGCTTTTCTCTTTACCCATTTCTCTACCCCTATTTCTAGACCATCCTCTATATATTCCATATCCAAAACCTCTAACCATGTTCTCTCAAAATCCCTGATCAAGTCTCACTCTACTGGGAAAGAGAATACGTTCGTTCTTGCTCCAGATGAGCGCTTGGGGGATTCGCATGCCGGGACTGAGCGTGAACAAATTGACAATTCAAGCACAATTGCTGCCATTGTGACATCCATTGGGGGTCCCCCTGCTGCTGTGGGGATTGTGCGTTTATCGGGTCCTCGTGCTGTAAATATCGTTGGGACTTTATTTTTCCCTGCCGCCAAAAAGAAGGGGAAAAATTTGAGCCTGCACCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGATGAAGTTTTGACAGTGCCAATGTTGGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGTCGTGTGCTAAAAGCTTGTTTAGAAGCTGGAGCTCGGCTTGCAGAACCAGGTGAGTTTACTCTTCGTGCCTTCTTAAATGGACGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCCGATGCTGCACTGGCTGGAATTCAGGGTGGCTTCTCTTCCTTAGTCAAATCGCTAAGAACACAATGTATCGAATTACTCACTGAAATTGAGGCTCGTCTAGACTTTGATGATGAGATGCCACCGTTGGATTTGAACATTGTAATGGAAAAAGTGCATGCCATGTCACAGGAAGTAGAGACTGCTCTTGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAATGTTACAGTTTCTGGTATTCCTGTGACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCAGATGTCATTATTATGGCAATAAGTGCCCTCGATGGATGGACTGCCGAAGATACCATACTTCTCAATAGGATACTTTCAAAAAAGAAATCAGATGAATCATGCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTCCAAAGATGGATGCAATGAGTATAAATCGTGATTCTTTCAGTAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACGGTAAACCAGAGACAATGCGTGCAGCTTCTTAGAACCAAGGAGGCATTTACAAGATTGAAATCATCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGATTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAG

Protein sequence

MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTGKENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK*
Homology
BLAST of CSPI05G14530 vs. ExPASy Swiss-Prot
Match: Q8KPU2 (tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=mnmE PE=3 SV=3)

HSP 1 Score: 437.2 bits (1123), Expect = 2.8e-121
Identity = 234/476 (49.16%), Postives = 322/476 (67.65%), Query Frame = 0

Query: 87  NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVV 146
           +  TIAAI T+I     +VGIVRLSG  A  I   +F  A ++        PW   SH +
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIFQIAGQQ--------PWE--SHRI 64

Query: 147 EYGVVLD-QQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGARLA 206
            YG + D + G ++DE L +PMLAPRSYTREDV+EL CHG  + +++ L+ C+ AGARLA
Sbjct: 65  LYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARLA 124

Query: 207 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCIEL 266
           EPGEFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C+++
Sbjct: 125 EPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLDI 184

Query: 267 LTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNV 326
           L E+EAR+DF+D++PPLDL  +  ++ A   +++  L TA+  +LL++G++IAIVGRPNV
Sbjct: 185 LAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPNV 244

Query: 327 GKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVE 386
           GKSSLLNAWS+ +RAIVT++ GTTRD++E+ + V GIPV +LDTAGIRET D VE+IGVE
Sbjct: 245 GKSSLLNAWSRCDRAIVTDLPGTTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVE 304

Query: 387 RSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPK 446
           RS  AA  AD++++ I A  GW+AED  +   +        S  PILLVINK D     +
Sbjct: 305 RSRRAAQSADLVLLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRDRLSEAE 364

Query: 447 MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLR 506
             A+++ +  F   V+T A   QGI++LE AI   VG     ++   W +NQRQ   L  
Sbjct: 365 RHAIALPQQEFKAIVWTAAAQQQGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTT 424

Query: 507 TKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 562
            + A  R++ +++ +LP DFWT+DLR+A+ ALG I GE+I+E +L  IF +FCIGK
Sbjct: 425 AQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEEIAESMLDLIFSRFCIGK 462

BLAST of CSPI05G14530 vs. ExPASy Swiss-Prot
Match: Q5N638 (tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=mnmE PE=3 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 9.1e-120
Identity = 232/476 (48.74%), Postives = 320/476 (67.23%), Query Frame = 0

Query: 87  NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVV 146
           +  TIAAI T+I     +VGIVRLSG  A  I   +F  A ++        PW   SH +
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIFQIAGQQ--------PWE--SHRI 64

Query: 147 EYGVVLD-QQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGARLA 206
            YG + D + G ++DE L +PMLAPRSYTREDV+EL CHG  + +++ L+ C+ AGARLA
Sbjct: 65  LYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARLA 124

Query: 207 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCIEL 266
           EPGEFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C+++
Sbjct: 125 EPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLDI 184

Query: 267 LTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNV 326
           L E+EAR+DF+D++PPLDL  +  ++ A   +++  L TA+  +LL++G++IAIVGRPNV
Sbjct: 185 LAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPNV 244

Query: 327 GKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVE 386
           GKSSLLNAWS+ +RAIVT++ G TRD++E+ + V GIPV +LDTAGIRET D VE+IGVE
Sbjct: 245 GKSSLLNAWSRCDRAIVTDLPGRTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVE 304

Query: 387 RSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPK 446
           RS  AA  AD++++ I A  GW+AED  +   +        S  PILLVINK D     +
Sbjct: 305 RSRRAAQSADLVLLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRDRLSEAE 364

Query: 447 MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLR 506
             A+++ +  F   V+T A   +GI++LE AI   VG     ++   W +NQRQ   L  
Sbjct: 365 RHAIALPQQEFKAIVWTAAAQQKGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTT 424

Query: 507 TKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 562
            + A  R++ +++ +LP DFWT+DLR+A+ ALG I GE I+E +L  IF +FCIGK
Sbjct: 425 AQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEGIAESMLDLIFSRFCIGK 462

BLAST of CSPI05G14530 vs. ExPASy Swiss-Prot
Match: B0CBB0 (tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=mnmE PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 4.2e-117
Identity = 230/476 (48.32%), Postives = 317/476 (66.60%), Query Frame = 0

Query: 89  STIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVVEY 148
           +TIAAI T+I     ++GIVRLSG  AV+I   LF    K++        W   SH V Y
Sbjct: 4   TTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQ--------WE--SHRVLY 63

Query: 149 GVV---LDQQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGARLA 208
           G +   L QQ  +IDE L + MLAPRSYTREDV+E  CHG  + +++VL+ACL+AGA LA
Sbjct: 64  GYIQQPLTQQ--IIDEGLLLLMLAPRSYTREDVVEFHCHGGMIAVQQVLEACLQAGAELA 123

Query: 209 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCIEL 268
           +PGEFTLRAFLNGRLDL+QAE V  L+ A+S  AA AALAG+QG  +S ++ LR +C++ 
Sbjct: 124 QPGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASPIRELRQRCLDT 183

Query: 269 LTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNV 328
           L E+EAR+DF+D++PPLD   V  ++  +   ++  L TA+  +LL++G+ +AI+GRPNV
Sbjct: 184 LAEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNV 243

Query: 329 GKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVE 388
           GKSSLLNAW + +RAIVT++ GTTRDV+E+ + V GIP+ +LDTAGIRET+D VE+IGV 
Sbjct: 244 GKSSLLNAWCRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVT 303

Query: 389 RSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPK 448
           RS  AA  AD++++ I A  GWT++D  L               P++L++NK+D  P  +
Sbjct: 304 RSHQAAQSADLVLLTIDASVGWTSDDQQLYQAFQD--------LPLILIVNKVDLVPQEQ 363

Query: 449 MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLR 508
           +    +  ++ ++ V T A   QGI  LE AI E V      A+   W +NQRQ   L +
Sbjct: 364 V----VYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQK 423

Query: 509 TKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 562
            + A   ++ +I D+LP DFWT+DLR A+ ALGEI GEDI+E +L  IF +FCIGK
Sbjct: 424 AQAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK 455

BLAST of CSPI05G14530 vs. ExPASy Swiss-Prot
Match: B8HSJ3 (tRNA modification GTPase MnmE OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=mnmE PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.6e-116
Identity = 233/488 (47.75%), Postives = 316/488 (64.75%), Query Frame = 0

Query: 85  IDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSH 144
           ++   TIAAI T+I     ++ IVRLSG  AV I   LF    ++         W   SH
Sbjct: 2   LNPGQTIAAIATAIVPQQGSIAIVRLSGSEAVAIAQRLFIAPGQQ--------VWE--SH 61

Query: 145 VVEYGVVLD-QQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGAR 204
            + YG V + + G  +DE L + MLAPRSYT+EDV+E  CHG  + +++VL+ CLEAGA 
Sbjct: 62  RILYGYVCNPRTGQRVDEALLLLMLAPRSYTKEDVVEFHCHGGMIAVQQVLQLCLEAGAV 121

Query: 205 LAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCI 264
           LA+PGEFTLRAFL+GRLDL+QAE+V  L+ AKS  AA AALAG+QG     ++ LR  CI
Sbjct: 122 LAQPGEFTLRAFLHGRLDLTQAESVADLVGAKSPQAAQAALAGLQGKLIQPLQQLRRTCI 181

Query: 265 ELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRP 324
           ++L EIEAR+DF++++PPLDLN +  ++     EV   L TA+  +LL++G+++AIVGRP
Sbjct: 182 DILAEIEARIDFEEDLPPLDLNQISSQIQHSLTEVNRILATADRGELLRTGLKVAIVGRP 241

Query: 325 NVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIG 384
           NVGKSSLLNAWS+S+RAIVTE+ GTTRDV+E+ + V GIPV +LDTAGIRET D VE+IG
Sbjct: 242 NVGKSSLLNAWSRSDRAIVTELPGTTRDVVESYLVVGGIPVQVLDTAGIRETSDQVEQIG 301

Query: 385 VERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKID---- 444
           VERS  AA  AD++++ I A  GWTAED  +  ++  +         ++LVINKID    
Sbjct: 302 VERSHKAAQAADLVLLVIDAQTGWTAEDQAIYTQVQERS--------LILVINKIDLVDQ 361

Query: 445 ------CAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRW 504
                 C P P +  +            T A   QGI++LE AI   V   +   +   W
Sbjct: 362 AFQPQACLPDPALTYLC-----------TAAAQNQGIEDLESAILSKVQGGELEGANLDW 421

Query: 505 TVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNI 562
            +NQRQ   L R K +   ++ +I ++LP DFWT+DLR+A+ ALGEI GE+++E +L  I
Sbjct: 422 AINQRQAAALTRAKLSLENVQETIANQLPLDFWTIDLREAIRALGEITGEEVTESVLDQI 460

BLAST of CSPI05G14530 vs. ExPASy Swiss-Prot
Match: Q8YN91 (tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=mnmE PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.6e-116
Identity = 224/475 (47.16%), Postives = 319/475 (67.16%), Query Frame = 0

Query: 88  SSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVVE 147
           + TIAAI T+I     +VGIVR+SG +A+ I  TLF    K+         W   SH + 
Sbjct: 4   TGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQ--------VWE--SHRIL 63

Query: 148 YGVVL-DQQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACLEAGARLAE 207
           YG +   Q   ++DE L + M APRSYTREDV+E  CHG  + +++VL+ CLE+GARLA+
Sbjct: 64  YGYIRHPQTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIIAVQQVLQLCLESGARLAQ 123

Query: 208 PGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSLRTQCIELL 267
           PGEFTLRAFLNGRLDL+QAE++  L+ A+S  AA  ALAG+QG  +  ++ LR  C+++L
Sbjct: 124 PGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDIL 183

Query: 268 TEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNVG 327
            EIEAR+DF++++PPLD   ++  +  ++ E+   L T +  +LL++G+++AIVGRPNVG
Sbjct: 184 AEIEARIDFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVG 243

Query: 328 KSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVER 387
           KSSLLNAWS+S+RAIVT++ GTTRDV+E+ + V GIPV +LDTAGIRET D VEKIGVER
Sbjct: 244 KSSLLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVER 303

Query: 388 SEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPKM 447
           S  AA  AD++++ I A  GWT  D  +  ++  +        P++LV+NKID      +
Sbjct: 304 SRQAANTADLVLLTIDAATGWTTGDQEIYEQVKHR--------PLILVMNKIDLVEKQLI 363

Query: 448 DAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLRT 507
            ++    ++ ++ V T A   QGI +LE AI E+V   K  A+     +NQRQ   L + 
Sbjct: 364 TSLEY-PENITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAALTQA 423

Query: 508 KEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 562
           K +  +++++I  +LP DFWT+DLR A+ ALGEI GE+++E +L  IF +FCIGK
Sbjct: 424 KMSLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK 459

BLAST of CSPI05G14530 vs. ExPASy TrEMBL
Match: A0A0A0KTK4 (TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET
Sbjct: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. ExPASy TrEMBL
Match: A0A1S3CCP4 (tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.1e-299
Identity = 538/561 (95.90%), Postives = 544/561 (96.97%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFL+THFSTPISRPSS+YSISKTSNHVLSKSLIKSHST 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHSTA 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTF+LAPDERL DSH G EREQI+NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLF PAAKKKGKNLS HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFCPAAKKKGKNLSRHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALA IQGGFSSLVK LR QCIELLTEIEARLDFDDEMPPLDLN+VMEKV AMSQEVET
Sbjct: 241 DAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIMAISA DGWT EDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESC PILLV+NKIDCAPSP MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQC QLLRTKEAFTRLKSSIEDELPPDFWTVDLR AVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. ExPASy TrEMBL
Match: A0A5D3DT93 (tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001540 PE=3 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.5e-298
Identity = 537/561 (95.72%), Postives = 543/561 (96.79%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFL+THFSTPISRPSS+YSISKTSNHVLSKSLIKSH T 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHFTA 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTF+LAPDERL DSH G EREQI+NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLF PAAKKKGKNLS HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFCPAAKKKGKNLSRHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALA IQGGFSSLVK LR QCIELLTEIEARLDFDDEMPPLDLN+VMEKV AMSQEVET
Sbjct: 241 DAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIMAISA DGWT EDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESC PILLV+NKIDCAPSP MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQC QLLRTKEAFTRLKSSIEDELPPDFWTVDLR AVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. ExPASy TrEMBL
Match: A0A6J1JUP0 (uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489063 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.1e-276
Identity = 505/563 (89.70%), Postives = 527/563 (93.61%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFI HF RTTPPMAFLFTHF TPISRPSS++ I   S+ VL KSLIKSH++G
Sbjct: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           K+ TF L  DERL DSHAG EREQI+NSSTIAAIVTS+GGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLS-LHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180
           +LF PAAKKKGKN+S  HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI
Sbjct: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVE 300
           ADAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPL+L  +MEKVHAM+QEVE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRIL 420
           SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWT EDTILL+ IL
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420

Query: 421 SKKKSDESCTPILLVINKIDCAPS-PKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAIS 480
           +KKKSD S TPILLVINKIDCAPS   MDA+SINRDSFSKQVFTCAVTGQGI++LEMAIS
Sbjct: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALG 540
           EL+GLN TLASGRRWTVNQRQC QLLRTKEA  RLKSSIE+ELP DFWTVDLR AVLALG
Sbjct: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 EICGEDISEEILSNIFGKFCIGK 562
           EI GEDISEE+LSNIFGKFCIGK
Sbjct: 541 EISGEDISEEVLSNIFGKFCIGK 563

BLAST of CSPI05G14530 vs. ExPASy TrEMBL
Match: A0A6J1FFU5 (uncharacterized protein LOC111445335 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445335 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 6.3e-273
Identity = 501/563 (88.99%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFI HF RTTPPM FL THF TPISRPSS++ I   S  VL KSLIKSH++G
Sbjct: 1   MALLPGFRHFITHFCRTTPPMPFLLTHFYTPISRPSSLFRIPNPSCPVLPKSLIKSHASG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KE TF L  DERL DSHAG EREQI+ SSTIAAIVTS+GGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KETTFFLTRDERLEDSHAGIEREQIETSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLS-LHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180
           +LF PAAKKKGK++S  HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI
Sbjct: 121 SLFCPAAKKKGKHVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVE 300
           ADAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPL+L  +MEKVHAM+QEVE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRIL 420
           SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWT EDTILL+ IL
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420

Query: 421 SKKKSDESCTPILLVINKIDCAPS-PKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAIS 480
           +KKKSD S TPILLVINKIDCAPS   MDA+SINRDSFSKQVFTCAVTGQGI++LEMAIS
Sbjct: 421 AKKKSDGSSTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALG 540
           EL+GLN TLA+GRRWTVNQRQC QLLRTKEA  RLKSSIE+ELP DFWTVDLR AVLALG
Sbjct: 481 ELMGLNNTLATGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 EICGEDISEEILSNIFGKFCIGK 562
           EI GEDISEE+LSNIFGKFCIGK
Sbjct: 541 EISGEDISEEVLSNIFGKFCIGK 563

BLAST of CSPI05G14530 vs. NCBI nr
Match: XP_004140317.1 (uncharacterized protein LOC101217353 isoform X1 [Cucumis sativus] >KGN51006.1 hypothetical protein Csa_007730 [Cucumis sativus])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET
Sbjct: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. NCBI nr
Match: XP_008460482.1 (PREDICTED: tRNA modification GTPase MnmE [Cucumis melo])

HSP 1 Score: 1036.2 bits (2678), Expect = 1.1e-298
Identity = 538/561 (95.90%), Postives = 544/561 (96.97%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFL+THFSTPISRPSS+YSISKTSNHVLSKSLIKSHST 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHSTA 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTF+LAPDERL DSH G EREQI+NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLF PAAKKKGKNLS HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFCPAAKKKGKNLSRHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALA IQGGFSSLVK LR QCIELLTEIEARLDFDDEMPPLDLN+VMEKV AMSQEVET
Sbjct: 241 DAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIMAISA DGWT EDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESC PILLV+NKIDCAPSP MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQC QLLRTKEAFTRLKSSIEDELPPDFWTVDLR AVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. NCBI nr
Match: TYK26555.1 (tRNA modification GTPase MnmE [Cucumis melo var. makuwa])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.2e-298
Identity = 537/561 (95.72%), Postives = 543/561 (96.79%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFL+THFSTPISRPSS+YSISKTSNHVLSKSLIKSH T 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHFTA 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTF+LAPDERL DSH G EREQI+NSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLF PAAKKKGKNLS HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFCPAAKKKGKNLSRHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALA IQGGFSSLVK LR QCIELLTEIEARLDFDDEMPPLDLN+VMEKV AMSQEVET
Sbjct: 241 DAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIMAISA DGWT EDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSDESC PILLV+NKIDCAPSP MDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL
Sbjct: 421 KKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQC QLLRTKEAFTRLKSSIEDELPPDFWTVDLR AVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. NCBI nr
Match: XP_038874821.1 (tRNA modification GTPase MnmE [Benincasa hispida])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.8e-288
Identity = 521/561 (92.87%), Postives = 533/561 (95.01%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFI HFYRTTPPMAFL THF TPISRPSS+ SI K SN VL KSLIK HSTG
Sbjct: 1   MALLPGFRHFITHFYRTTPPMAFLLTHFHTPISRPSSLSSIPKPSNPVLPKSLIKFHSTG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTFVLAPDERL DSHAG EREQI+NSSTIAA+VTS+GG PAAVGIVRLSGPRAV+IVG
Sbjct: 61  KENTFVLAPDERLADSHAGIEREQIENSSTIAAVVTSMGGSPAAVGIVRLSGPRAVDIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           +LF PA KKKGKNLSLHPWRPTSHVVEYGVVLD+QGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 SLFCPAGKKKGKNLSLHPWRPTSHVVEYGVVLDKQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPL L+I+MEKVH MSQEVE 
Sbjct: 241 DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLVLDIIMEKVHTMSQEVEA 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWTAEDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTAEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KKKSD S TPILLVINKIDCAPSP MDA+SIN DSFSKQVFTCAVTGQGI+NLEM ISEL
Sbjct: 421 KKKSDGSSTPILLVINKIDCAPSPNMDAISINHDSFSKQVFTCAVTGQGIENLEMTISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQC QLLRTKEA TRLKSSIEDELP DFWTVDLRDA LALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEALTRLKSSIEDELPLDFWTVDLRDAALALGEI 540

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEE+LSNIFGKFCIGK
Sbjct: 541 CGEDISEEVLSNIFGKFCIGK 561

BLAST of CSPI05G14530 vs. NCBI nr
Match: XP_031741993.1 (uncharacterized protein LOC101217353 isoform X2 [Cucumis sativus])

HSP 1 Score: 961.4 bits (2484), Expect = 3.3e-276
Identity = 512/561 (91.27%), Postives = 512/561 (91.27%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120
           KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180
           TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE
Sbjct: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300
           DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET
Sbjct: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420

Query: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480
           KK                                                 NLEMAISEL
Sbjct: 421 KK-------------------------------------------------NLEMAISEL 480

Query: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540
           VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI
Sbjct: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 512

Query: 541 CGEDISEEILSNIFGKFCIGK 562
           CGEDISEEILSNIFGKFCIGK
Sbjct: 541 CGEDISEEILSNIFGKFCIGK 512

BLAST of CSPI05G14530 vs. TAIR 10
Match: AT1G78010.1 (tRNA modification GTPase, putative )

HSP 1 Score: 670.6 bits (1729), Expect = 1.1e-192
Identity = 365/570 (64.04%), Postives = 429/570 (75.26%), Query Frame = 0

Query: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60
           M  L  FR    +  R   P+       S PIS+       S +    L K   +S    
Sbjct: 1   MVSLLCFRSVGVYLLRQATPIITRGGRISRPISKNLCFVLFSSSPKTSLLKPRAQS---S 60

Query: 61  KENTFVLAPDERLGD------SHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPR 120
           + N+ V   DER+          A  + ++  +SSTI AIVT IGGPP AVGIVRLSGP+
Sbjct: 61  ENNSLVFKGDERVVGLVGKVVDDAFDKVDRFQSSSTIVAIVTPIGGPPGAVGIVRLSGPK 120

Query: 121 AVNIVGTLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYT 180
           AV +   +F  A K K K      WRP SH VEYG V+D  G+V+DEVL VPMLAPRSYT
Sbjct: 121 AVEVARRVFRSAKKTKKKESDSDTWRPKSHFVEYGAVVDSNGNVVDEVLAVPMLAPRSYT 180

Query: 181 REDVIELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISA 240
           REDV+ELQCHGSEVCLRRVL+ C+EAGARLAEPGEFTLRAFLNGRLDLSQAENV KLISA
Sbjct: 181 REDVVELQCHGSEVCLRRVLRTCVEAGARLAEPGEFTLRAFLNGRLDLSQAENVEKLISA 240

Query: 241 KSTAAADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAM 300
           KS+AAADAAL GIQGGFSSLVKSLR QCIELLTEIEARLDF+DEMPPLD+  V+ K+ +M
Sbjct: 241 KSSAAADAALEGIQGGFSSLVKSLRAQCIELLTEIEARLDFEDEMPPLDIESVINKITSM 300

Query: 301 SQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIE 360
           SQ+VE+AL+TANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTE+AGTTRDV+E
Sbjct: 301 SQDVESALDTANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEVAGTTRDVVE 360

Query: 361 ANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTIL 420
           ANVTV G+P+TLLDTAGIRET+DIVEKIGVERSE AA  ADVIIMA+SA++GWT EDT L
Sbjct: 361 ANVTVRGVPITLLDTAGIRETNDIVEKIGVERSETAAKVADVIIMAVSAVEGWTEEDTEL 420

Query: 421 LNRILSKKKSDESCTPILLVINKIDCAPSPKMDAMSINR---DSFSKQVFTCAVTGQGIQ 480
           L +I S K       P++LV+NKIDCAP    D +   R   + F K VFT AVTGQGI+
Sbjct: 421 LRKIQSDK-------PMILVMNKIDCAPPGSCDQLEDQRKKEEVFHKSVFTSAVTGQGIE 480

Query: 481 NLEMAISELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLR 540
            LE AI E++GL++    G +WTVNQRQC QL+RTKEA  RL+ +IEDE+P DFWT++LR
Sbjct: 481 ELEDAILEILGLDRVPTGGHQWTVNQRQCEQLVRTKEALVRLREAIEDEIPIDFWTIELR 540

Query: 541 DAVLALGEICGEDISEEILSNIFGKFCIGK 562
           +A L+L +I G+D+SEE+LS+IF KFCIGK
Sbjct: 541 EAALSLAQISGQDVSEEVLSSIFAKFCIGK 560

BLAST of CSPI05G14530 vs. TAIR 10
Match: AT3G12080.1 (GTP-binding family protein )

HSP 1 Score: 87.0 bits (214), Expect = 5.1e-17
Identity = 60/176 (34.09%), Postives = 95/176 (53.98%), Query Frame = 0

Query: 317 IAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVT-VSGIPVTLLDTAGIRET 376
           IAI+GRPNVGKSS+LNA  + +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+ 
Sbjct: 371 IAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKK 430

Query: 377 DDI------VEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCT 436
             +       E + V R+  A   +DV+ + I A+   T +D  +  RI   ++  + C 
Sbjct: 431 SSVASSGSTTEAMSVNRAFRAIRRSDVVALVIEAMACITEQDLKIAERI---EREGKGC- 490

Query: 437 PILLVINKIDCAPSPKMDAMSINRDSFSKQ---------VFTCAVTGQGIQNLEMA 477
             L+V+NK D  P+   +  +   D   ++         V++ A+TG  + N+ +A
Sbjct: 491 --LVVVNKWDTIPNKNQETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVA 540

BLAST of CSPI05G14530 vs. TAIR 10
Match: AT3G12080.2 (GTP-binding family protein )

HSP 1 Score: 87.0 bits (214), Expect = 5.1e-17
Identity = 60/176 (34.09%), Postives = 95/176 (53.98%), Query Frame = 0

Query: 317 IAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVT-VSGIPVTLLDTAGIRET 376
           IAI+GRPNVGKSS+LNA  + +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+ 
Sbjct: 371 IAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKK 430

Query: 377 DDI------VEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCT 436
             +       E + V R+  A   +DV+ + I A+   T +D  +  RI   ++  + C 
Sbjct: 431 SSVASSGSTTEAMSVNRAFRAIRRSDVVALVIEAMACITEQDLKIAERI---EREGKGC- 490

Query: 437 PILLVINKIDCAPSPKMDAMSINRDSFSKQ---------VFTCAVTGQGIQNLEMA 477
             L+V+NK D  P+   +  +   D   ++         V++ A+TG  + N+ +A
Sbjct: 491 --LVVVNKWDTIPNKNQETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVA 540

BLAST of CSPI05G14530 vs. TAIR 10
Match: AT5G39960.1 (GTP binding;GTP binding )

HSP 1 Score: 65.9 bits (159), Expect = 1.2e-10
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 0

Query: 315 IQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAG--- 374
           +Q+AIVG+PNVGKS+LLNA  + ER +V   AG TRD +       G  V L+DTAG   
Sbjct: 318 LQLAIVGKPNVGKSTLLNALLEEERVLVGPEAGLTRDAVRVQFEFQGRTVYLVDTAGWLE 377

Query: 375 IRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPI 434
             E D     + + +S  + + A VI + + A +   A+ ++  + ++  +++ E    +
Sbjct: 378 RTERDKGPASLSIMQSRKSLMRAHVIALVLDAEEIIKAKCSMTHSEVVIARRAVEEGRGL 437

Query: 435 LLVINKID 440
           ++++NK+D
Sbjct: 438 VVIVNKMD 445

BLAST of CSPI05G14530 vs. TAIR 10
Match: AT5G66470.1 (RNA binding;GTP binding )

HSP 1 Score: 46.6 bits (109), Expect = 7.7e-05
Identity = 47/173 (27.17%), Postives = 81/173 (46.82%), Query Frame = 0

Query: 268 EIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIAIVGRPNVGK 327
           E +  +D  DE   L L++  ++  A+  + E   E  +          +A+VG PNVGK
Sbjct: 84  EEQVEIDIVDEASLLSLSMKPDRNMALLDDYEME-ELGHTPNTHHRSGYVAVVGMPNVGK 143

Query: 328 SSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETD-DIVEKIGVER 387
           S+L N     + +IVT+   TTR  I    +     + L DT G+ E     ++ + ++ 
Sbjct: 144 STLSNQMIGQKISIVTDKPQTTRHRILGICSSPEYQMILYDTPGVIEKKMHRLDTMMMKN 203

Query: 388 SEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKID 440
              AA+ AD +++ + A    T  + +L   +   +K      P+LLV+NK D
Sbjct: 204 VRDAAINADCVVILVDACKTPTNIEEVLKEGLGDLEKK----PPMLLVMNKKD 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8KPU22.8e-12149.16tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACH... [more]
Q5N6389.1e-12048.74tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301... [more]
B0CBB04.2e-11748.32tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329... [more]
B8HSJ33.6e-11647.75tRNA modification GTPase MnmE OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) O... [more]
Q8YN913.6e-11647.16tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX ... [more]
Match NameE-valueIdentityDescription
A0A0A0KTK40.0e+00100.00TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040... [more]
A0A1S3CCP45.1e-29995.90tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1[more]
A0A5D3DT932.5e-29895.72tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1JUP06.1e-27689.70uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FFU56.3e-27388.99uncharacterized protein LOC111445335 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_004140317.10.0e+00100.00uncharacterized protein LOC101217353 isoform X1 [Cucumis sativus] >KGN51006.1 hy... [more]
XP_008460482.11.1e-29895.90PREDICTED: tRNA modification GTPase MnmE [Cucumis melo][more]
TYK26555.15.2e-29895.72tRNA modification GTPase MnmE [Cucumis melo var. makuwa][more]
XP_038874821.13.8e-28892.87tRNA modification GTPase MnmE [Benincasa hispida][more]
XP_031741993.13.3e-27691.27uncharacterized protein LOC101217353 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G78010.11.1e-19264.04tRNA modification GTPase, putative [more]
AT3G12080.15.1e-1734.09GTP-binding family protein [more]
AT3G12080.25.1e-1734.09GTP-binding family protein [more]
AT5G39960.11.2e-1032.81GTP binding;GTP binding [more]
AT5G66470.17.7e-0527.17RNA binding;GTP binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027368tRNA modification GTPase MnmE domain 2GENE3D1.20.120.430tRNA modification GTPase MnmE domain 2coord: 220..561
e-value: 2.5E-97
score: 328.6
IPR025867MnmE, helical domainPFAMPF12631MnmE_helicalcoord: 222..558
e-value: 4.3E-46
score: 158.1
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 314..470
e-value: 8.6E-23
score: 78.9
IPR027266GTP-binding protein TrmE/Glycine cleavage system T protein, domain 1GENE3D3.30.1360.120Probable tRNA modification gtpase trme; domain 1coord: 75..219
e-value: 3.9E-43
score: 148.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 315..489
e-value: 2.5E-97
score: 328.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 172..477
IPR018948GTP-binding protein TrmE, N-terminalPFAMPF10396TrmE_Ncoord: 90..219
e-value: 1.9E-36
score: 125.0
IPR004520tRNA modification GTPase MnmETIGRFAMTIGR00450TIGR00450coord: 103..561
e-value: 5.3E-118
score: 392.9
IPR004520tRNA modification GTPase MnmEHAMAPMF_00379GTPase_MnmEcoord: 89..561
score: 30.712908
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 316..437
e-value: 1.3E-23
score: 83.3
NoneNo IPR availablePIRSRPIRSR038134-2PIRSR038134-2coord: 312..380
e-value: 0.0052
score: 13.7
NoneNo IPR availablePANTHERPTHR42714TRNA MODIFICATION GTPASE GTPBP3coord: 88..561
NoneNo IPR availablePANTHERPTHR42714:SF2TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIALcoord: 88..561
IPR031168TrmE-type guanine nucleotide-binding domainPROSITEPS51709G_TRMEcoord: 314..482
score: 58.792366
IPR031168TrmE-type guanine nucleotide-binding domainCDDcd04164trmEcoord: 312..482
e-value: 7.0708E-66
score: 209.658

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G14530.1CSPI05G14530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0002098 tRNA wobble uridine modification
biological_process GO:0006400 tRNA modification
cellular_component GO:0005829 cytosol
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005515 protein binding