CSPI05G14070 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G14070
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cold shock protein 2-like
Location	Chr5: 14299702 .. 14301046 (+)
RNA-Seq Expression	CSPI05G14070
Synteny	CSPI05G14070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTCCCCCGAATCTCTCTATCCGAGAGACATCCACTTTCGAAAAGTGAATCAATACTCAAATAAAGGGGAGGAAAAATCAATTGTCCGCCATTGGTTCCTTCTGTGAATGACATGCAGCTTATTCATCTAGATATACATTGCTACAAGCTTTTCAATTGTCCTTTCTTTCCCTTTTCTTTCTTTTACACTTTTTCCTTTTCATGGCTGAGCTGAAGAGATCGACCGGTACTGTAAGGTGGTTTAGTGCACAGAAGGGGTTTGGCTTCATAGCTCCAGATGATGCTACCGATGATCTTTTCGTCCACCAAACCTCCATTCGTTCAGAAGGATTCCGGACTCTTTTCGATGGTCAAACTGTTGAGTTTACCATCGATTATGATCAAGATCAACGCGCCAAGGCCGTCGACGTCACTGTAATTGATAGATCTACCAGATATGGAGGAGGCCGCGGGGGTGGGAGAGGTAGAGGTGGTTCTTATGGAAGATTCGGTGGTGGTGGACGTGGTTTTGGCAGAGGAAGATGGAACGAAGGTCCAACCGGTGGTGGTCGTGGCGGTGGGGAGTGCTACAATTGTGGCCGGATGGGTCATTTGGCGAGGGATTGTTACAGGGGTAATGGCGGTGCTCCTTCCGGCCGTAGATACGACGGTGGTCGAGGTTATGGTGGAGGTGGAGGCGGCAGAGGTTGTTATAATTGTGGAGATACAGGACATTTGGCTAGGGATTGTCAAAACGAAAGCAAATGAAGAATGTTGGTTTTCCTTTAAGCGGTACACATTATACTTTTTCTGAAAATTATTTTGGTTGGGCTGTTAATTTTTTTATGCGAAATCTGGGATTTTTTAGTGTGTCAGTGACTTCAAGTCTGCAATTGAGGGAACGAGGATCTGGTGGCAATAAATCTTAAGGCTTCAGTTCGTCTATATTTCTCCTTTTGGGTCTTCCATAATTTGATGTTTATAATTAGATGATGAAACCAATTGTTTGTATATGCTATACCTTTCTGGCATTTCTAATTCATTCTTTCTTTGCTTGTTTTATAGAATCATAATATGATATTTGTAATCTGTCAAAGGTATACGTTCATACCATTTTTTATTTCTTGTATACGTTTCACTTTTGTCTATTACTGTAATGGTTGTATTTTTTCGCCCCCATTATTGATTTCTTGGTGCTTTACTGGGATTCTTTGCTTCGAAACTTCAATCATTGGTGTTGAATTGGCAATGGAAGTAATTGTCCTGCACTATGTACTATATTTTTGCCTGTGTTTTCCCCGGAATCTGAATGAAACCAGAGCTCTTAATAATACTTTAGATATCTTTGAAGTTTCTCACAGAGG

mRNA sequence

CTTTTCCCCCGAATCTCTCTATCCGAGAGACATCCACTTTCGAAAAGTGAATCAATACTCAAATAAAGGGGAGGAAAAATCAATTGTCCGCCATTGGTTCCTTCTGTGAATGACATGCAGCTTATTCATCTAGATATACATTGCTACAAGCTTTTCAATTGTCCTTTCTTTCCCTTTTCTTTCTTTTACACTTTTTCCTTTTCATGGCTGAGCTGAAGAGATCGACCGGTACTGTAAGGTGGTTTAGTGCACAGAAGGGGTTTGGCTTCATAGCTCCAGATGATGCTACCGATGATCTTTTCGTCCACCAAACCTCCATTCGTTCAGAAGGATTCCGGACTCTTTTCGATGGTCAAACTGTTGAGTTTACCATCGATTATGATCAAGATCAACGCGCCAAGGCCGTCGACGTCACTGTAATTGATAGATCTACCAGATATGGAGGAGGCCGCGGGGGTGGGAGAGGTAGAGGTGGTTCTTATGGAAGATTCGGTGGTGGTGGACGTGGTTTTGGCAGAGGAAGATGGAACGAAGGTCCAACCGGTGGTGGTCGTGGCGGTGGGGAGTGCTACAATTGTGGCCGGATGGGTCATTTGGCGAGGGATTGTTACAGGGGTAATGGCGGTGCTCCTTCCGGCCGTAGATACGACGGTGGTCGAGGTTATGGTGGAGGTGGAGGCGGCAGAGGTTGTTATAATTGTGGAGATACAGGACATTTGGCTAGGGATTGTCAAAACGAAAGCAAATGAAGAATGTTGGTTTTCCTTTAAGCGGTACACATTATACTTTTTCTGAAAATTATTTTGGTTGGGCTGTTAATTTTTTTATGCGAAATCTGGGATTTTTTAGTGTGTCAGTGACTTCAAGTCTGCAATTGAGGGAACGAGGATCTGGTGGCAATAAATCTTAAGGCTTCAGTTCGTCTATATTTCTCCTTTTGGGTCTTCCATAATTTGATGTTTATAATTAGATGATGAAACCAATTGTTTGTATATGCTATACCTTTCTGGCATTTCTAATTCATTCTTTCTTTGCTTGTTTTATAGAATCATAATATGATATTTGTAATCTGTCAAAGGTATACGTTCATACCATTTTTTATTTCTTGTATACGTTTCACTTTTGTCTATTACTGTAATGGTTGTATTTTTTCGCCCCCATTATTGATTTCTTGGTGCTTTACTGGGATTCTTTGCTTCGAAACTTCAATCATTGGTGTTGAATTGGCAATGGAAGTAATTGTCCTGCACTATGTACTATATTTTTGCCTGTGTTTTCCCCGGAATCTGAATGAAACCAGAGCTCTTAATAATACTTTAGATATCTTTGAAGTTTCTCACAGAGG

Coding sequence (CDS)

ATGGCTGAGCTGAAGAGATCGACCGGTACTGTAAGGTGGTTTAGTGCACAGAAGGGGTTTGGCTTCATAGCTCCAGATGATGCTACCGATGATCTTTTCGTCCACCAAACCTCCATTCGTTCAGAAGGATTCCGGACTCTTTTCGATGGTCAAACTGTTGAGTTTACCATCGATTATGATCAAGATCAACGCGCCAAGGCCGTCGACGTCACTGTAATTGATAGATCTACCAGATATGGAGGAGGCCGCGGGGGTGGGAGAGGTAGAGGTGGTTCTTATGGAAGATTCGGTGGTGGTGGACGTGGTTTTGGCAGAGGAAGATGGAACGAAGGTCCAACCGGTGGTGGTCGTGGCGGTGGGGAGTGCTACAATTGTGGCCGGATGGGTCATTTGGCGAGGGATTGTTACAGGGGTAATGGCGGTGCTCCTTCCGGCCGTAGATACGACGGTGGTCGAGGTTATGGTGGAGGTGGAGGCGGCAGAGGTTGTTATAATTGTGGAGATACAGGACATTTGGCTAGGGATTGTCAAAACGAAAGCAAATGA

Protein sequence

MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGRGGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK*

Homology

[Close]

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot
Match: Q41188 (Cold shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=CSP2 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-39
Identity = 102/199 (51.26%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R  G+V+WF  QKGFGFI PDD  DDLFVHQ+SIRSEGFR+L   + VEF ++ D + R
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNNNR 68

Query: 65  AKAVDVTVIDRST--------------RYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNE 124
            KA+DV+  D +                +GGGRGGGRG GG Y   GGGG G+G      
Sbjct: 69  PKAIDVSGPDGAPVQGNSGGGSSGGRGGFGGGRGGGRGSGGGY---GGGGGGYG------ 128

Query: 125 GPTGGGRGGGECYNCGRMGHLARDCYRGNGG-APSGRRYDGGRGYGGGGGGRG------- 177
           G  GGGRGG +CY CG  GH+ARDC  G GG    G  Y GG GYGGGGGG G       
Sbjct: 129 GRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYGGGGGYGGGGGGYGGGGRGGG 188

[Close]

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot
Match: P27484 (Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 105/210 (50.00%), Postives = 123/210 (58.57%), Query Frame = 0


Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R+ GTV+WFS QKGFGFI PDD  +DLFVHQ+ IRSEGFR+L +G+TVEF ++   D R
Sbjct: 7   QRAKGTVKWFSDQKGFGFITPDDGGEDLFVHQSGIRSEGFRSLAEGETVEFEVESGGDGR 66

Query: 65  AKAVDVTVID----RSTRYGGGRGGGR---------------GRGGSYG----------- 124
            KAVDVT  D    +  R GGG GGGR               GRGGS G           
Sbjct: 67  TKAVDVTGPDGAAVQGGRGGGGGGGGRGGGGYGGGSGGYGGGGRGGSRGYGGGDGGYGGG 126

Query: 125 -------RFGGGGRGFGRGRWNEGPTGGGRGGGE-CYNCGRMGHLARDCYRGNGGAPSGR 177
                  R+GGGG G+G G    G  GGG GGG  C+ CG  GH ARDC +  GG   G 
Sbjct: 127 GGYGGGSRYGGGGGGYGGG---GGYGGGGSGGGSGCFKCGESGHFARDCSQSGGGG-GGG 186

[Close]

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot
Match: Q38896 (Cold shock domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=CSP4 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 8.5e-35
Identity = 93/182 (51.10%), Postives = 111/182 (60.99%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           R  GTV+WF  QKGFGFI P D  DDLFVHQ+SIRSEGFR+L   ++VEF ++ D   R 
Sbjct: 14  RRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNSGRP 73

Query: 66  KAVDVTVIDRSTRYG--------GGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGR 125
           KA++V+  D +   G        GGRGG  G GG  G  GGG  G G G    G  GGG 
Sbjct: 74  KAIEVSGPDGAPVQGNSGGGGSSGGRGGFGGGGGRGGGRGGGSYGGGYGGRGSGGRGGGG 133

Query: 126 GGGECYNCGRMGHLARDCYRGNG---GAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 177
           G   C+ CG  GH+AR+C +G G   G   G RY  G G GGGGGG  CY+CG++GH AR
Sbjct: 134 GDNSCFKCGEPGHMARECSQGGGGYSGGGGGGRYGSGGGGGGGGGGLSCYSCGESGHFAR 193

[Close]

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot
Match: Q94C69 (Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.5e-26
Identity = 86/188 (45.74%), Postives = 108/188 (57.45%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RS G V WFS  KG+GFI PDD  ++LFVHQ+SI S+GFR+L  G++VE+ I    D + 
Sbjct: 10  RSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIALGSDGKT 69

Query: 66  KAVDVTV-----IDRSTRYGGGRGGGRGRGGSYGRF------GGGGRGFGRGRWNEGPTG 125
           KA++VT      +++      G GG     G  G        G GG+ FG G       G
Sbjct: 70  KAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGG-------G 129

Query: 126 GGRGG--GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHL 181
           G R G  GECY CG +GH ARDC R +GG  SG         GGGGGGR CY+CG+ GHL
Sbjct: 130 GRRSGGEGECYMCGDVGHFARDC-RQSGGGNSG---------GGGGGGRPCYSCGEVGHL 180

[Close]

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot
Match: O65639 (Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.0e-24
Identity = 82/183 (44.81%), Postives = 98/183 (53.55%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RSTG V WF+A KG+GFI PDD + +LFVHQ+SI SEG+R+L  G  VEF I    D + 
Sbjct: 10  RSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQGSDGKT 69

Query: 66  KAVDVTV-------IDRSTRYGGGRGGGRGRG-GSYGRFGGGGRGFGRGRWNEGPTGGGR 125
           KAV+VT         + ++R  G R GG G G  + G  G   +  G G    G     R
Sbjct: 70  KAVNVTAPGGGSLKKENNSRGNGARRGGGGSGCYNCGELGHISKDCGIGGGGGGGERRSR 129

Query: 126 GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQ 181
           GG  CYNCG  GH ARDC     G   G            GG  GCY CGD GH+ARDC 
Sbjct: 130 GGEGCYNCGDTGHFARDCTSAGNGDQRG---------ATKGGNDGCYTCGDVGHVARDCT 183

[Close]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL
Match: A0A0A0KRF7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G374720 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.0e-95
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGRGGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGP+GGGRGGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120

Query: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180
           ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES
Sbjct: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180

Query: 181 K 182
           K
Sbjct: 181 K 181

[Close]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL
Match: A0A1S3CPN9 (cold shock protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503390 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.6e-90
Identity = 172/182 (94.51%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPT-GGGRGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GP+ GGGRGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

Query: 181 SK 182
           SK
Sbjct: 181 SK 182

[Close]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL
Match: A0A5A7TX13 (Cold shock protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold507G00260 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.6e-90
Identity = 172/182 (94.51%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPT-GGGRGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GP+ GGGRGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

Query: 181 SK 182
           SK
Sbjct: 181 SK 182

[Close]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL
Match: A0A6J1K351 (cold shock protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489638 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 4.6e-76
Identity = 160/196 (81.63%), Postives = 167/196 (85.20%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPT-- 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GP+  
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ----GGGRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGYGGGGGGRGCYN 180
               GGGRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGYGGGGGGRGCYN
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGRGCYN 180

Query: 181 CGDTGHLARDCQNESK 182
           CGDTGH ARDCQNESK
Sbjct: 181 CGDTGHFARDCQNESK 196

[Close]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL
Match: A0A6J1FIQ9 (cold shock protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444649 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.5e-74
Identity = 160/198 (80.81%), Postives = 167/198 (84.34%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPT-- 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GP+  
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ----GGGRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGY--GGGGGGRGC 180
               GGGRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGY  GGGGGGRGC
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGGGRGC 180

Query: 181 YNCGDTGHLARDCQNESK 182
           YNCGDTGH ARDCQNESK
Sbjct: 181 YNCGDTGHFARDCQNESK 198

[Close]

BLAST of CSPI05G14070 vs. NCBI nr
Match: XP_004140332.1 (cold shock protein 2 [Cucumis sativus] >XP_011655173.1 cold shock protein 2 [Cucumis sativus])

HSP 1 Score: 358.2 bits (918), Expect = 4.1e-95
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGRGGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGP+GGGRGGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120

Query: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180
           ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES
Sbjct: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180

Query: 181 K 182
           K
Sbjct: 181 K 181

[Close]

BLAST of CSPI05G14070 vs. NCBI nr
Match: KGN51220.2 (hypothetical protein Csa_008318 [Cucumis sativus])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 175/177 (98.87%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR
Sbjct: 322 RRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 381

Query: 65  AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGRGGGECYN 124
           AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGP+GGGRGGGECYN
Sbjct: 382 AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGGECYN 441

Query: 125 CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK 182
           CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK
Sbjct: 442 CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK 498

[Close]

BLAST of CSPI05G14070 vs. NCBI nr
Match: XP_008465791.1 (PREDICTED: cold shock protein 2-like [Cucumis melo] >XP_008465792.1 PREDICTED: cold shock protein 2-like [Cucumis melo] >KAA0047448.1 cold shock protein 2-like [Cucumis melo var. makuwa] >TYJ98642.1 cold shock protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 172/182 (94.51%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPT-GGGRGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GP+ GGGRGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

Query: 181 SK 182
           SK
Sbjct: 181 SK 182

[Close]

BLAST of CSPI05G14070 vs. NCBI nr
Match: XP_038906549.1 (glycine-rich protein 2-like [Benincasa hispida])

HSP 1 Score: 302.0 bits (772), Expect = 3.5e-78
Identity = 160/187 (85.56%), Postives = 169/187 (90.37%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA++DLFVHQTSIRSEGFRTLFDGQ VEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASEDLFVHQTSIRSEGFRTLFDGQAVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGR-GRWNE-----GPTG 120
           +DQR+KAVDVTVI RST YGGGRGGGRGRGG+YGRFGGGGRGFGR GR+ +     G  G
Sbjct: 61  EDQRSKAVDVTVIGRSTGYGGGRGGGRGRGGAYGRFGGGGRGFGRGGRYEDSSGGFGGRG 120

Query: 121 GGRGGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 180
           GGRGGGECYNCGRMGHLARDCYRGNGGAP GR Y GGRGYGGG GG GCYNCGDTGH AR
Sbjct: 121 GGRGGGECYNCGRMGHLARDCYRGNGGAPPGRGYGGGRGYGGGAGG-GCYNCGDTGHFAR 180

Query: 181 DCQNESK 182
           DCQNESK
Sbjct: 181 DCQNESK 186

[Close]

BLAST of CSPI05G14070 vs. NCBI nr
Match: XP_022993723.1 (cold shock protein 2-like [Cucurbita maxima] >KAG6578982.1 Cold shock protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016506.1 Cold shock protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293.9 bits (751), Expect = 9.6e-76
Identity = 160/196 (81.63%), Postives = 167/196 (85.20%), Query Frame = 0


Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPT-- 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GP+  
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ----GGGRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGYGGGGGGRGCYN 180
               GGGRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGYGGGGGGRGCYN
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGRGCYN 180

Query: 181 CGDTGHLARDCQNESK 182
           CGDTGH ARDCQNESK
Sbjct: 181 CGDTGHFARDCQNESK 196

[Close]

BLAST of CSPI05G14070 vs. TAIR 10
Match: AT4G38680.1 (glycine rich protein 2 )

HSP 1 Score: 163.7 bits (413), Expect = 1.4e-40
Identity = 102/199 (51.26%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R  G+V+WF  QKGFGFI PDD  DDLFVHQ+SIRSEGFR+L   + VEF ++ D + R
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNNNR 68

Query: 65  AKAVDVTVIDRST--------------RYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNE 124
            KA+DV+  D +                +GGGRGGGRG GG Y   GGGG G+G      
Sbjct: 69  PKAIDVSGPDGAPVQGNSGGGSSGGRGGFGGGRGGGRGSGGGY---GGGGGGYG------ 128

Query: 125 GPTGGGRGGGECYNCGRMGHLARDCYRGNGG-APSGRRYDGGRGYGGGGGGRG------- 177
           G  GGGRGG +CY CG  GH+ARDC  G GG    G  Y GG GYGGGGGG G       
Sbjct: 129 GRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYGGGGGYGGGGGGYGGGGRGGG 188

[Close]

BLAST of CSPI05G14070 vs. TAIR 10
Match: AT2G21060.1 (glycine-rich protein 2B )

HSP 1 Score: 148.3 bits (373), Expect = 6.1e-36
Identity = 93/182 (51.10%), Postives = 111/182 (60.99%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           R  GTV+WF  QKGFGFI P D  DDLFVHQ+SIRSEGFR+L   ++VEF ++ D   R 
Sbjct: 14  RRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNSGRP 73

Query: 66  KAVDVTVIDRSTRYG--------GGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPTGGGR 125
           KA++V+  D +   G        GGRGG  G GG  G  GGG  G G G    G  GGG 
Sbjct: 74  KAIEVSGPDGAPVQGNSGGGGSSGGRGGFGGGGGRGGGRGGGSYGGGYGGRGSGGRGGGG 133

Query: 126 GGGECYNCGRMGHLARDCYRGNG---GAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 177
           G   C+ CG  GH+AR+C +G G   G   G RY  G G GGGGGG  CY+CG++GH AR
Sbjct: 134 GDNSCFKCGEPGHMARECSQGGGGYSGGGGGGRYGSGGGGGGGGGGLSCYSCGESGHFAR 193

[Close]

BLAST of CSPI05G14070 vs. TAIR 10
Match: AT2G17870.1 (cold shock domain protein 3 )

HSP 1 Score: 119.0 bits (297), Expect = 3.9e-27
Identity = 86/188 (45.74%), Postives = 108/188 (57.45%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RS G V WFS  KG+GFI PDD  ++LFVHQ+SI S+GFR+L  G++VE+ I    D + 
Sbjct: 10  RSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIALGSDGKT 69

Query: 66  KAVDVTV-----IDRSTRYGGGRGGGRGRGGSYGRF------GGGGRGFGRGRWNEGPTG 125
           KA++VT      +++      G GG     G  G        G GG+ FG G       G
Sbjct: 70  KAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGG-------G 129

Query: 126 GGRGG--GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHL 181
           G R G  GECY CG +GH ARDC R +GG  SG         GGGGGGR CY+CG+ GHL
Sbjct: 130 GRRSGGEGECYMCGDVGHFARDC-RQSGGGNSG---------GGGGGGRPCYSCGEVGHL 180

[Close]

BLAST of CSPI05G14070 vs. TAIR 10
Match: AT4G36020.1 (cold shock domain protein 1 )

HSP 1 Score: 112.8 bits (281), Expect = 2.8e-25
Identity = 82/183 (44.81%), Postives = 98/183 (53.55%), Query Frame = 0


Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RSTG V WF+A KG+GFI PDD + +LFVHQ+SI SEG+R+L  G  VEF I    D + 
Sbjct: 10  RSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQGSDGKT 69

Query: 66  KAVDVTV-------IDRSTRYGGGRGGGRGRG-GSYGRFGGGGRGFGRGRWNEGPTGGGR 125
           KAV+VT         + ++R  G R GG G G  + G  G   +  G G    G     R
Sbjct: 70  KAVNVTAPGGGSLKKENNSRGNGARRGGGGSGCYNCGELGHISKDCGIGGGGGGGERRSR 129

Query: 126 GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQ 181
           GG  CYNCG  GH ARDC     G   G            GG  GCY CGD GH+ARDC 
Sbjct: 130 GGEGCYNCGDTGHFARDCTSAGNGDQRG---------ATKGGNDGCYTCGDVGHVARDCT 183

The following BLAST results are available for this feature:

BLAST of CSPI05G14070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41188	2.0e-39	51.26	Cold shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=CSP2 PE=1 SV=1	[more]
P27484	1.3e-35	50.00	Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1	[more]
Q38896	8.5e-35	51.10	Cold shock domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=CSP4 P...	[more]
Q94C69	5.5e-26	45.74	Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 P...	[more]
O65639	4.0e-24	44.81	Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1	[more]

BLAST of CSPI05G14070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KRF7	2.0e-95	99.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G374720 PE=4 SV=1	[more]
A0A1S3CPN9	5.6e-90	94.51	cold shock protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503390 PE=4 SV=1	[more]
A0A5A7TX13	5.6e-90	94.51	Cold shock protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1K351	4.6e-76	81.63	cold shock protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489638 PE=4 SV=1	[more]
A0A6J1FIQ9	1.5e-74	80.81	cold shock protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444649 PE=4 SV=...	[more]

BLAST of CSPI05G14070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140332.1	4.1e-95	99.45	cold shock protein 2 [Cucumis sativus] >XP_011655173.1 cold shock protein 2 [Cuc...	[more]
KGN51220.2	1.5e-92	98.87	hypothetical protein Csa_008318 [Cucumis sativus]	[more]
XP_008465791.1	1.2e-89	94.51	PREDICTED: cold shock protein 2-like [Cucumis melo] >XP_008465792.1 PREDICTED: c...	[more]
XP_038906549.1	3.5e-78	85.56	glycine-rich protein 2-like [Benincasa hispida]	[more]
XP_022993723.1	9.6e-76	81.63	cold shock protein 2-like [Cucurbita maxima] >KAG6578982.1 Cold shock protein 1,...	[more]

BLAST of CSPI05G14070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38680.1	1.4e-40	51.26	glycine rich protein 2	[more]
AT2G21060.1	6.1e-36	51.10	glycine-rich protein 2B	[more]
AT2G17870.1	3.9e-27	45.74	cold shock domain protein 3	[more]
AT4G36020.1	2.8e-25	44.81	cold shock domain protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002059	Cold-shock protein, DNA-binding	PRINTS	PR00050	COLDSHOCK	coord: 30..39 score: 58.54 coord: 45..63 score: 35.07 coord: 9..24 score: 60.88
IPR002059	Cold-shock protein, DNA-binding	PFAM	PF00313	CSD	coord: 8..72 e-value: 1.4E-22 score: 79.3
IPR002059	Cold-shock protein, DNA-binding	PROSITE	PS51857	CSD_2	coord: 6..72 score: 30.728745
IPR002059	Cold-shock protein, DNA-binding	CDD	cd04458	CSP_CDS	coord: 8..72 e-value: 3.80468E-23 score: 85.323
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 121..137 e-value: 2.7E-4 score: 30.2 coord: 162..178 e-value: 1.2E-5 score: 34.7
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 161..177 e-value: 1.2E-7 score: 31.5 coord: 121..135 e-value: 6.2E-7 score: 29.2
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 122..135 score: 11.070239
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 163..178 score: 11.086722
IPR011129	Cold shock domain	SMART	SM00357	csp_8	coord: 8..73 e-value: 1.1E-22 score: 91.4
None	No IPR available	GENE3D	2.40.50.140		coord: 1..71 e-value: 1.5E-24 score: 87.6
None	No IPR available	GENE3D	4.10.60.10		coord: 115..152 e-value: 1.0E-6 score: 30.2 coord: 158..181 e-value: 2.3E-6 score: 29.1
None	No IPR available	PANTHER	PTHR46565	COLD SHOCK DOMAIN PROTEIN 2	coord: 1..179
IPR019844	Cold-shock (CSD) domain	PROSITE	PS00352	CSD_1	coord: 20..39
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 5..73
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 111..139
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 145..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G14070.1	CSPI05G14070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding