CSPI05G13600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G13600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
LocationChr5: 13411586 .. 13413376 (-)
RNA-Seq ExpressionCSPI05G13600
SyntenyCSPI05G13600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATCATTTCCTGGAACACAAGAGGCCTAAAAGATCCAAATAAACGTTCCGCTCTTAAGAAGTTTATAAAAAATCATCACCCGGACATGGTGCTAATCCAAGAATCAAAAATGGAAATTCTGGAAGTAAATTTCATTAAAACAATTTGGAGTTCTATGGATATAGGATGGGAATCAGTGGAATCTTATGGCGCTTCTGGAGGCATTCTTACCCTATGGGATAAAAGTAAAATCACAGTGGTGGAAACCATAAGAAGACATTATTCCCTCTCAATAAAATGTGTAACTTTGTGCAAGAAGTGTTGTTGGGTTACCAATGTTTATGGTCCATGTGGTTACAGAGAGAGGAAACTTGTTTGGCCAGAATTATTAACAATTGCAGAATGTGGAGAAGAGGCTTGGTCTTTGGGAGGAGATTTTAATATCACTAGATGGGTCTATGAGAGGTTTCCAGTTGGCAGAAGCACAAGAGGAATGAGACAGTTTAATGCCTTTATAGATTCTGCCAATCTAATGGAAATTTCCCTTCAAAATGGCAAATTTACTTGGTCAAGAGAGGATCGCAGTGCTGCAAGATCTCTGTTGGACAGATTTTTTATTAACAGTAAATGGGATGATGAATTTGAAAACTCAAGAGTGACCCGTAAAGCAAGAACCTTCTTCGATCACTTTCCTTTATTATTAGAGGCAGGTGCAATACTATGGGGGCCATCCCCATTCAGATTTTGCAATAGTTGGTTGTAGATAAAAGAGTGCAACAAGGTTATTGAGGAAGTCTTGAAAATCGCCCTCCCAGACATTGGGTGGGCTGTTTTCATTCTTCATGACGTAAAGTGAAATTTGCTGTTAAAAATTGGCATGCTACTCATTTGATTGATACAAAAGCAGAAAGAGGAAAGGCTTTTGAGAGAGTTAGAAATCATTGATGGATTTGCAGAAAGTGTTGGTCTGAATGAAGTGGAATTGGCTCACAAGTCAGCTATACAAACAAAGTTGCTTTGTATTTACAGAACCGAAGAAATAAATTTTATACAGAAAAATAAACTAAATTGGCTTGCTATAGGAGATGAAAATACGGGCTTTTTTCACAGATTCCTTAATGCAAAAAAAAGGAAAAACCTAATCACAAAATTGGTGGATGAGCAAGGGGTGATAACGAAGTCTTTTCTCGACATTGAAAGGGTGATATTGTGGTTCTATGAAGACCTGTATAAAAAAATTCTAGGAGTCAGATATCTTCCTTTTATTGCAGATTGGCCTTGTGTCTCTGTTTCTCAAAAATTTTCACTAATCAACAGTTTCTCTGCGGTGGAGATTTTCAAGGCAATTAAAGCATTAGGAGGCAATAAAGCACCCAGACCGGATGGTTTTACTACAGAATTTTTTGTGAAACACTGGCCAGTTCTAAAAGAAGGTTTGATGAGATTGTTTGAAGAATTCCACAGAAATGGTAAGCTCAATGCTTGCATTCAAGAAAACTTCATTTGCTTAATTCAGAAGAAAGAAGATGCGATTCATGTTAGAGACTTCAGACTCATTAGTCTCACAACCATCACTTACAAGGTGGTTGCCAAGGTACTAGCAGATAGGCTTAAAGGGGTAATGGATTCAATAATCAGCCCTTTTCAAAGCGCGTTCATTGAAGGTAGACAGGTTTTAGACCCCATTCTTATAGCTAACGAAGTTGTAGAAGACTATCAAGCCAAAGGGAAAAAAGGGTGGATTTTGAAACTAGACCTTGAAAAGGCCTTTGATAGGGTGGATTGGGGCTTCCTCGTAAAAGTTTAG

mRNA sequence

ATGAAGATCATTTCCTGGAACACAAGAGGCCTAAAAGATCCAAATAAACGTTCCGCTCTTAAGAAGTTTATAAAAAATCATCACCCGGACATGGTGCTAATCCAAGAATCAAAAATGGAAATTCTGGAAGTAAATTTCATTAAAACAATTTGGAGTTCTATGGATATAGGATGGGAATCAGTGGAATCTTATGGCGCTTCTGGAGGCATTCTTACCCTATGGGATAAAAGTAAAATCACAGTGGTGGAAACCATAAGAAGACATTATTCCCTCTCAATAAAATGTGTAACTTTGTGCAAGAAGTGTTGTTGGGTTACCAATGTTTATGGTCCATGTGGTTACAGAGAGAGGAAACTTGTTTGGCCAGAATTATTAACAATTGCAGAATGTGGAGAAGAGGCTTGGTCTTTGGGAGGAGATTTTAATATCACTAGATGGGTCTATGAGAGGTTTCCAGTTGGCAGAAGCACAAGAGGAATGAGACAGTTTAATGCCTTTATAGATTCTGCCAATCTAATGGAAATTTCCCTTCAAAATGGCAAATTTACTTGGTCAAGAGAGGATCGCAGTGCTGCAAGATCTCTGTTGGACAGATTTTTTATTAACAGTAAATGGGATGATGAATTTGAAAACTCAAGAATAAAAGAGTGCAACAAGGTTATTGAGGAAGTCTTGAAAATCGCCCTCCCAGACATTGGGTGGGCTAAAGAGGAAAGGCTTTTGAGAGAGTTAGAAATCATTGATGGATTTGCAGAAAGTGTTGGTCTGAATGAAGTGGAATTGGCTCACAAATTCCTTAATGCAAAAAAAAGGAAAAACCTAATCACAAAATTGGTGGATGAGCAAGGGGTGATAACGAAGTCTTTTCTCGACATTGAAAGGGTGATATTGTGGTTCTATGAAGACCTGTATAAAAAAATTCTAGGAGTCAGATATCTTCCTTTTATTGCAGATTGGCCTTGTGTCTCTGTTTCTCAAAAATTTTCACTAATCAACAGTTTCTCTGCGGTGGAGATTTTCAAGGCAATTAAAGCATTAGGAGGCAATAAAGCACCCAGACCGGATGGTTTTACTACAGAATTTTTTGTGAAACACTGGCCAGTTCTAAAAGAAGGTTTGATGAGATTGTTTGAAGAATTCCACAGAAATGGTAAGCTCAATGCTTGCATTCAAGAAAACTTCATTTGCTTAATTCAGAAGAAAGAAGATGCGATTCATGTTAGAGACTTCAGACTCATTAGTCTCACAACCATCACTTACAAGGTGGTTGCCAAGGTACTAGCAGATAGGCTTAAAGGGGTAATGGATTCAATAATCAGCCCTTTTCAAAGCGCGTTCATTGAAGGTAGACAGGTTTTAGACCCCATTCTTATAGCTAACGAAGTTGTAGAAGACTATCAAGCCAAAGGGAAAAAAGGGTGGATTTTGAAACTAGACCTTGAAAAGGCCTTTGATAGGGTGGATTGGGGCTTCCTCGTAAAAGTTTAG

Coding sequence (CDS)

ATGAAGATCATTTCCTGGAACACAAGAGGCCTAAAAGATCCAAATAAACGTTCCGCTCTTAAGAAGTTTATAAAAAATCATCACCCGGACATGGTGCTAATCCAAGAATCAAAAATGGAAATTCTGGAAGTAAATTTCATTAAAACAATTTGGAGTTCTATGGATATAGGATGGGAATCAGTGGAATCTTATGGCGCTTCTGGAGGCATTCTTACCCTATGGGATAAAAGTAAAATCACAGTGGTGGAAACCATAAGAAGACATTATTCCCTCTCAATAAAATGTGTAACTTTGTGCAAGAAGTGTTGTTGGGTTACCAATGTTTATGGTCCATGTGGTTACAGAGAGAGGAAACTTGTTTGGCCAGAATTATTAACAATTGCAGAATGTGGAGAAGAGGCTTGGTCTTTGGGAGGAGATTTTAATATCACTAGATGGGTCTATGAGAGGTTTCCAGTTGGCAGAAGCACAAGAGGAATGAGACAGTTTAATGCCTTTATAGATTCTGCCAATCTAATGGAAATTTCCCTTCAAAATGGCAAATTTACTTGGTCAAGAGAGGATCGCAGTGCTGCAAGATCTCTGTTGGACAGATTTTTTATTAACAGTAAATGGGATGATGAATTTGAAAACTCAAGAATAAAAGAGTGCAACAAGGTTATTGAGGAAGTCTTGAAAATCGCCCTCCCAGACATTGGGTGGGCTAAAGAGGAAAGGCTTTTGAGAGAGTTAGAAATCATTGATGGATTTGCAGAAAGTGTTGGTCTGAATGAAGTGGAATTGGCTCACAAATTCCTTAATGCAAAAAAAAGGAAAAACCTAATCACAAAATTGGTGGATGAGCAAGGGGTGATAACGAAGTCTTTTCTCGACATTGAAAGGGTGATATTGTGGTTCTATGAAGACCTGTATAAAAAAATTCTAGGAGTCAGATATCTTCCTTTTATTGCAGATTGGCCTTGTGTCTCTGTTTCTCAAAAATTTTCACTAATCAACAGTTTCTCTGCGGTGGAGATTTTCAAGGCAATTAAAGCATTAGGAGGCAATAAAGCACCCAGACCGGATGGTTTTACTACAGAATTTTTTGTGAAACACTGGCCAGTTCTAAAAGAAGGTTTGATGAGATTGTTTGAAGAATTCCACAGAAATGGTAAGCTCAATGCTTGCATTCAAGAAAACTTCATTTGCTTAATTCAGAAGAAAGAAGATGCGATTCATGTTAGAGACTTCAGACTCATTAGTCTCACAACCATCACTTACAAGGTGGTTGCCAAGGTACTAGCAGATAGGCTTAAAGGGGTAATGGATTCAATAATCAGCCCTTTTCAAAGCGCGTTCATTGAAGGTAGACAGGTTTTAGACCCCATTCTTATAGCTAACGAAGTTGTAGAAGACTATCAAGCCAAAGGGAAAAAAGGGTGGATTTTGAAACTAGACCTTGAAAAGGCCTTTGATAGGGTGGATTGGGGCTTCCTCGTAAAAGTTTAG

Protein sequence

MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWESVESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLVWPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNGKFTWSREDRSAARSLLDRFFINSKWDDEFENSRIKECNKVIEEVLKIALPDIGWAKEERLLRELEIIDGFAESVGLNEVELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIADWPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEEFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSIISPFQSAFIEGRQVLDPILIANEVVEDYQAKGKKGWILKLDLEKAFDRVDWGFLVKV*
Homology
BLAST of CSPI05G13600 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-21
Identity = 58/174 (33.33%), Postives = 93/174 (53.45%), Query Frame = 0

Query: 320 PCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEE 379
           P VS  +K  L    +  E+ +A++ +  NK+P  DG T EFF   W  L     R+  E
Sbjct: 435 PVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTE 494

Query: 380 FHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSII 439
             + G+L    +   + L+ KK D   ++++R +SL +  YK+VAK ++ RLK V+  +I
Sbjct: 495 AFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVI 554

Query: 440 SPFQSAFIEGRQVLDPILIANEVVEDYQAKGKKGWILKLDLEKAFDRVDWGFLV 494
            P QS  + GR + D + +  +++   +  G     L LD EKAFDRVD  +L+
Sbjct: 555 HPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLI 608

BLAST of CSPI05G13600 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 5.0e-21
Identity = 89/312 (28.53%), Postives = 147/312 (47.12%), Query Frame = 0

Query: 189 RSAARSLLDRFFINSKWDDEFENSRIKECNKVIEEVLKIALPDIGWAKEERLLRELEIID 248
           R   RS +D      K  ++ E +  K   +  +E+ KI         ++ L +  E   
Sbjct: 307 RKQERSKIDTLTSQLKELEKQEQTHSKASRR--QEITKIRAELKEIETQKTLQKINESRS 366

Query: 249 GFAESVGLNEVELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLY-KKI 308
            F E +   +  LA + +  K+ KN I  + +++G IT    +I+  I  +Y+ LY  K+
Sbjct: 367 WFFERINKIDRPLA-RLIKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKL 426

Query: 309 LGVRYLPFIAD---WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVK 368
             +  +    D    P ++  +  SL    +  EI   I +L   K+P PDGFT EF+ +
Sbjct: 427 ENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQR 486

Query: 369 HWPVLKEGLMRLFEEFHRNGKLNACIQENFICLIQKK-EDAIHVRDFRLISLTTITYKVV 428
           +   L   L++LF+   + G L     E  I LI K   D     +FR ISL  I  K++
Sbjct: 487 YKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKIL 546

Query: 429 AKVLADRLKGVMDSIISPFQSAFIEGRQVLDPILIANEVVEDY-QAKGKKGWILKLDLEK 488
            K+LA+R++  +  +I   Q  FI G Q    I  +  V++   +AK K   I+ +D EK
Sbjct: 547 NKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEK 606

Query: 489 AFDRVDWGFLVK 495
           AFD++   F++K
Sbjct: 607 AFDKIQQPFMLK 615

BLAST of CSPI05G13600 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 5.6e-20
Identity = 86/281 (30.60%), Postives = 138/281 (49.11%), Query Frame = 0

Query: 222 EEVLKIALPDIGWAKEERLLREL-EIIDGFAESVGLNEVELAHKFLNAKKRKNLITKLVD 281
           +E++K+   +I   +  R ++ + +    F E +   +  LA +     + K LI K+ +
Sbjct: 345 QEIIKLR-GEINQVETRRTIQRINQTRSWFFEKINKIDKPLA-RLTKGHRDKILINKIRN 404

Query: 282 EQGVITKSFLDIERVILWFYEDLYK-KILGVRYLPFIAD---WPCVSVSQKFSLINSFSA 341
           E+G IT    +I+  I  FY+ LY  K+  +  +    D    P ++  Q   L +  S 
Sbjct: 405 EKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVPKLNQDQVDHLNSPISP 464

Query: 342 VEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEEFHRNGKLNACIQENFIC 401
            EI   I +L   K+P PDGF+ EF+      L   L +LF +    G L     E  I 
Sbjct: 465 KEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGTLPNSFYEATIT 524

Query: 402 LIQK-KEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSIISPFQSAFIEGRQVLDP 461
           LI K ++D   + +FR ISL  I  K++ K+LA+R++  + +II P Q  FI G Q    
Sbjct: 525 LIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFN 584

Query: 462 ILIANEVVEDY-QAKGKKGWILKLDLEKAFDRVDWGFLVKV 496
           I  +  V+    + K K   I+ LD EKAFD++   F++KV
Sbjct: 585 IRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKV 623

BLAST of CSPI05G13600 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 83/291 (28.52%), Postives = 140/291 (48.11%), Query Frame = 0

Query: 212 SRIKECNKVIEEVLKIALPDIGWAKEERLLREL-EIIDGFAESVGLNEVELAHKFLNAKK 271
           SR KE  K+  E+ +I        + +R+++++ +    F E +   +  LA+  L  KK
Sbjct: 334 SRRKEITKIRAELNEI--------ENKRIIQQINKSKSWFFEKINKIDKPLAN--LTRKK 393

Query: 272 R-KNLITKLVDEQGVITKSFLDIERVILWFYEDL----YKKILGVRYLPFIADWPCVSVS 331
           R K+LI+ + +    IT    +I++++  +Y+ L    Y+ +  +         P +S  
Sbjct: 394 RVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQK 453

Query: 332 QKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEEFHRNGK 391
           +   L    S+ EI   I+ L   K+P PDGFT+EF+      L   L+ LF+   + G 
Sbjct: 454 EVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGI 513

Query: 392 LNACIQENFICLIQKK-EDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSIISPFQS 451
           L     E  I LI K  +D     ++R ISL  I  K++ K+L +R++  +  II   Q 
Sbjct: 514 LPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQV 573

Query: 452 AFIEGRQVLDPILIANEVVEDY-QAKGKKGWILKLDLEKAFDRVDWGFLVK 495
            FI G Q    I  +  V++   + K K   IL +D EKAFD +   F+++
Sbjct: 574 GFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAFDNIQHPFMIR 614

BLAST of CSPI05G13600 vs. ExPASy TrEMBL
Match: A0A803P8A0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.1e-83
Identity = 192/594 (32.32%), Postives = 290/594 (48.82%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKI++WN RG  D  KR+A+K  I   +PDMV++QE K   ++  FI +IW S    W  
Sbjct: 1   MKILTWNIRGSGDKGKRAAIKATICKANPDMVILQEVKRATVDRRFIGSIWRSRFKAWIL 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + G SGG L +WD   I+V++++   +S+S+      K+  W + VYGPC Y+ R + 
Sbjct: 61  LPAIGRSGGTLLIWDTRIISVLDSLVGEFSISVLINAEGKEPWWFSGVYGPCSYKIRHVF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  ++    E+W +GGDFN+TR V E+     STR M+ F+  I    L++  L+NG
Sbjct: 121 WDELAGLSSICGESWCVGGDFNVTRRVGEKLNSSSSTRSMKLFDGLIRELQLIDPKLENG 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDDEFENSRIKECNKVIEE----VLKIALPDIG--- 240
            FTWS        S LDRF   + W+  F   R +   +++ +    V+    P  G   
Sbjct: 181 SFTWSNFRAIPICSRLDRFLFLNNWNVVFPFVRQEMLVRLVSDHSPVVIDSKPPKWGPGP 240

Query: 241 -------------------WAKEE--------RLLRELEIIDGFAE-----SVGLNEV-- 300
                              W +EE        + +++L+ + G A+     + G N+   
Sbjct: 241 FRFDNHWLEHKSFSKCFESWWQEEIIDGWPGTKFMKKLKTLQGKAKEWSRFTYGQNKATK 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 NALEGRLGVLDRQEGTPSWNQSLYDERRKLKEEWQRLTFEEERSIWLKSKCKWAKEGDAN 360

Query: 361 -ELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
               H  LNA+K +N I+++  + G I  S  +I   ++ F+  LY     +       +
Sbjct: 361 SRFFHNLLNARKARNTISRIERDNGDIIDSEKEIVEELIAFFSKLYTSETRMGTGVEGIE 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  ++      L   F   E+   + +  G+KAP PDGF+   F  +W V+K  LM +F 
Sbjct: 421 WQHIAEPSARQLECPFEEDEVRNIVFSCEGSKAPGPDGFSLAVFQNNWEVIKNELMEVFR 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 493
            FH  G++   I + FICLI K+ ++  V+DFR ISL T  YK++AK LA RL+GV+   
Sbjct: 481 AFHSEGRIEGSINDTFICLIPKRLNSCKVKDFRPISLITSVYKIIAKTLATRLRGVLGET 540

BLAST of CSPI05G13600 vs. ExPASy TrEMBL
Match: A0A803QEA6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 5.3e-82
Identity = 187/593 (31.53%), Postives = 287/593 (48.40%), Query Frame = 0

Query: 2   KIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWESV 61
           +I++WN RG  D  KR+A+K  I   +PD+V++QE K   ++  FI +IW S    W  +
Sbjct: 352 EILTWNIRGSGDKGKRTAIKATICKANPDLVILQEVKRATVDRRFIGSIWRSRFKAWILI 411

Query: 62  ESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLVW 121
            + G SGG L +WD   I+V++++   +S+S+      K+  W + VYGPC Y+ R   W
Sbjct: 412 PAIGRSGGTLLIWDTRTISVLDSLVGEFSISVLINAEGKEPWWFSGVYGPCSYKLRPEFW 471

Query: 122 PELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNGK 181
            EL  ++    ++W + GDFN+TR V E+      TR M+ F+  I    L++  L+NG 
Sbjct: 472 DELAGLSSICGKSWCVAGDFNVTRRVGEKLNSSSFTRSMKLFDGLIRELQLIDPKLENGS 531

Query: 182 FTWSREDRSAARSLLDRFFINSKWDDEFENSRIKECNKVIEEVLKIAL----PDIG---- 241
           FTWS    S   S LDRF   + W+  F   R +   +++ +   + +    P  G    
Sbjct: 532 FTWSNFRASPVCSRLDRFLFTNNWNIIFPFVRQELLVRIVSDHSPVVIDSNPPKWGPGPF 591

Query: 242 ------------------WAKEE--------RLLRELEIIDGFAE-----SVGLNEVE-- 301
                             W KEE        + +++L+I+ G  +     + G N  +  
Sbjct: 592 RFDNHWLDHKSFSKCFERWWKEEINDGWPGTKFMKKLKILQGKVKEWSKSTFGQNRAKKI 651

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 652 ALEGRLGVLDKLEGTSFWNQSLLDERRKLKEEWKWLNFEEERGTWLKSKCKWAREGDANS 711

Query: 362 -LAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIADW 421
              H  LNA+K +N I+++  E G I  +  +I   ++ F+  LY     +       +W
Sbjct: 712 RFFHNLLNARKARNTISRIERENGDIIDNEKEIAEELIAFFSKLYTSEARMGSGIEGIEW 771

Query: 422 PCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEE 481
             ++ S    L   F   E+   + +  GNKAP PDGF+      +W  +K  LM +F  
Sbjct: 772 QQIAESSAGQLECPFEEEEVRNIVFSCEGNKAPGPDGFSLAVLQHNWETIKHDLMEVFTA 831

Query: 482 FHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSII 493
           FHR G++   I + FICLI K+ ++  V+DFR ISL T  YK++AK LA RL+GV+   I
Sbjct: 832 FHREGRIEGSINDTFICLIPKRLNSCKVKDFRPISLITSVYKIIAKTLATRLRGVLGETI 891

BLAST of CSPI05G13600 vs. ExPASy TrEMBL
Match: A0A438CWL6 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_846 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 3.4e-81
Identity = 192/597 (32.16%), Postives = 282/597 (47.24%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F++   PD+V+IQE+K E  +   + ++W+  +  W+ 
Sbjct: 1   MKIISWNVRGLGSSNKRRVIKDFLRLEKPDVVMIQETKKEKCDRRLVGSVWTVRNKDWDF 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD  K++  E +   +S+S+K         W++ VYGP     RK  
Sbjct: 61  LPACGASGGILFIWDSKKLSKEEVVLGSFSISVKFALEGCGPLWISAVYGPNSPSLRKDF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+    R T  MR F++FI  + L++  L+N 
Sbjct: 121 WVELYDICGLTFPLWCVGGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNA 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
            FTWS    S     LDRF  +++W                                   
Sbjct: 181 SFTWSNMQESPVCKRLDRFLYSNEWGQLFPQGTQETLIRRTSDHWPIALDTNPFTWGPTP 240

Query: 241 --------------------------------------EFENSRIKECNK----VIEEVL 300
                                                 +F  ++ KE NK    V+ E  
Sbjct: 241 FRFENMWLQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKK 300

Query: 301 KIALPDI----------GWAKE---ERLLRELEIIDGFAE--------------SVGLNE 360
           K  L D+          G   E   +R LR+ E+ D                    G   
Sbjct: 301 KSILKDLANLDAIEQDGGLTSELLGQRALRKGELEDLILREEIHWRQKARVKWVKEGDCN 360

Query: 361 VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
            +  HK  N ++ +  I  L +E G++  + + I   IL ++E LY   +G  +     D
Sbjct: 361 SKFFHKVANGRRNRKYIKSLENETGLVLNNAMSITEEILLYFEKLYANPIGESWSIEGLD 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  +S     SL+  F+  EI KAI  +  +KAP PDGFT   F   W V+KE L+R+F 
Sbjct: 421 WSPISEESAISLVAPFTEEEISKAIFQMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFA 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 496
           EFHR+G +N     +FI L+ KK     + DFR ISL T  YK++AKVL+ RL+GV+   
Sbjct: 481 EFHRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHET 540

BLAST of CSPI05G13600 vs. ExPASy TrEMBL
Match: A0A438KGJ2 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_193 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 7.6e-81
Identity = 190/597 (31.83%), Postives = 280/597 (46.90%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F++   PD+V+IQE+K E  +   + ++W+  +  W  
Sbjct: 1   MKIISWNVRGLGSSNKRREIKDFLRLEKPDVVMIQETKKEKCDKRLVGSVWTVRNKDWVI 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD  K++  E +   +S+S+K V       W++ VYGP     RK  
Sbjct: 61  LSACGASGGILFIWDSKKLSKEEVVLGSFSISVKFVLEGCGPLWISTVYGPNSPSLRKDF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+    R T  MR F++FI  + L++  L+N 
Sbjct: 121 WVELYDIYGLTFPLWCVGGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNA 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
            FTWS    S     LDRF  +++W                                   
Sbjct: 181 SFTWSNMQESPMCKRLDRFLYSNEWGQLFPQGIQETLIRRTSDHWPIALDTNPFMWGPTP 240

Query: 241 --------------------------------------EFENSRIKECNK----VIEEVL 300
                                                 +F  ++ KE NK    V+ E  
Sbjct: 241 FRFENMWLQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKK 300

Query: 301 KIALPDIG--------WAKEERLLRELEIIDGFAESVGLNE------------------- 360
           K  L D+               LL +  +  G  E + L E                   
Sbjct: 301 KSILKDLANLDAIEQDGGLTSELLGQRALRKGELEELILREEIHWRQKARVKWVKEGDCN 360

Query: 361 VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
            +  HK  N ++ +  I  L +E G++  + + I   IL ++E LY   +G  +     D
Sbjct: 361 SKFFHKVANGRRNRKYIKSLENETGLVLNNAVSITEEILLYFEKLYANPIGESWSIEGLD 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  +S     SL+  F+  EI KAI  +  +KAP PDGFT   F   W V+KE L+R+F 
Sbjct: 421 WSPISEESAISLVAPFTEEEISKAIFQMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFA 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 496
           EFHR+G +N     +FI L+ KK     + DFR ISL T  YK++AKVL+ RL+GV+   
Sbjct: 481 EFHRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHET 540

BLAST of CSPI05G13600 vs. ExPASy TrEMBL
Match: A0A438FWU5 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_70 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.7e-80
Identity = 188/594 (31.65%), Postives = 283/594 (47.64%), Query Frame = 0

Query: 1    MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
            MKI+SWNTRGL    KR  +++F+   +PD+V++QE+K E  +  F+ ++W    + W +
Sbjct: 736  MKILSWNTRGLGSKKKRRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKGKRVEWAA 795

Query: 61   VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
            + + GASGGI+ LWD SK+   E +   +S+++K  +  +   W+T+VYGP     RK  
Sbjct: 796  LPACGASGGIVILWDSSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDF 855

Query: 121  WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
            W EL  +       W +GGDFN+ R + E+    R T  MR F+ FI  + L++  L+N 
Sbjct: 856  WLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNA 915

Query: 181  KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
             FTWS          LDRF  +S+WD                                  
Sbjct: 916  AFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTP 975

Query: 241  -EFEN-------------------------------------SRIKECNKV----IEEVL 300
              FEN                                     S++KE N +    ++E  
Sbjct: 976  FRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERK 1035

Query: 301  KIALPDIGWAK-------------EERLLRELEIIDGFAE--------------SVGLNE 360
            K+ L D+                  ER L+  E+ D   +                G   
Sbjct: 1036 KLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELEDVLLKEEVQWRQKSRVKWIKEGDCN 1095

Query: 361  VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
             +  H+    ++ +  I  L+ E+G    +  DI   I+ F+ +LY K +G  +     D
Sbjct: 1096 SKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFGNLYSKPVGESWRVEGID 1155

Query: 421  WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
            W  +S      L   F+  E+ +A+  L   KAP PDGFT   + + W V+KE LMR+F 
Sbjct: 1156 WVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPGPDGFTIAVYQECWDVIKEDLMRVFL 1215

Query: 481  EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 493
            EFH NG +N      FI L+ KK  ++ + D+R ISL T  YK++AKVL+ RL+ V+   
Sbjct: 1216 EFHTNGVINQSTNATFIALVPKKSQSVKISDYRPISLVTSLYKIIAKVLSGRLRKVLHET 1275

BLAST of CSPI05G13600 vs. NCBI nr
Match: RVW27595.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 312.4 bits (799), Expect = 7.1e-81
Identity = 192/597 (32.16%), Postives = 282/597 (47.24%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F++   PD+V+IQE+K E  +   + ++W+  +  W+ 
Sbjct: 1   MKIISWNVRGLGSSNKRRVIKDFLRLEKPDVVMIQETKKEKCDRRLVGSVWTVRNKDWDF 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD  K++  E +   +S+S+K         W++ VYGP     RK  
Sbjct: 61  LPACGASGGILFIWDSKKLSKEEVVLGSFSISVKFALEGCGPLWISAVYGPNSPSLRKDF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+    R T  MR F++FI  + L++  L+N 
Sbjct: 121 WVELYDICGLTFPLWCVGGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNA 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
            FTWS    S     LDRF  +++W                                   
Sbjct: 181 SFTWSNMQESPVCKRLDRFLYSNEWGQLFPQGTQETLIRRTSDHWPIALDTNPFTWGPTP 240

Query: 241 --------------------------------------EFENSRIKECNK----VIEEVL 300
                                                 +F  ++ KE NK    V+ E  
Sbjct: 241 FRFENMWLQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKK 300

Query: 301 KIALPDI----------GWAKE---ERLLRELEIIDGFAE--------------SVGLNE 360
           K  L D+          G   E   +R LR+ E+ D                    G   
Sbjct: 301 KSILKDLANLDAIEQDGGLTSELLGQRALRKGELEDLILREEIHWRQKARVKWVKEGDCN 360

Query: 361 VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
            +  HK  N ++ +  I  L +E G++  + + I   IL ++E LY   +G  +     D
Sbjct: 361 SKFFHKVANGRRNRKYIKSLENETGLVLNNAMSITEEILLYFEKLYANPIGESWSIEGLD 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  +S     SL+  F+  EI KAI  +  +KAP PDGFT   F   W V+KE L+R+F 
Sbjct: 421 WSPISEESAISLVAPFTEEEISKAIFQMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFA 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 496
           EFHR+G +N     +FI L+ KK     + DFR ISL T  YK++AKVL+ RL+GV+   
Sbjct: 481 EFHRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHET 540

BLAST of CSPI05G13600 vs. NCBI nr
Match: RVX20328.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 311.2 bits (796), Expect = 1.6e-80
Identity = 190/597 (31.83%), Postives = 280/597 (46.90%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F++   PD+V+IQE+K E  +   + ++W+  +  W  
Sbjct: 1   MKIISWNVRGLGSSNKRREIKDFLRLEKPDVVMIQETKKEKCDKRLVGSVWTVRNKDWVI 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD  K++  E +   +S+S+K V       W++ VYGP     RK  
Sbjct: 61  LSACGASGGILFIWDSKKLSKEEVVLGSFSISVKFVLEGCGPLWISTVYGPNSPSLRKDF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+    R T  MR F++FI  + L++  L+N 
Sbjct: 121 WVELYDIYGLTFPLWCVGGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNA 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
            FTWS    S     LDRF  +++W                                   
Sbjct: 181 SFTWSNMQESPMCKRLDRFLYSNEWGQLFPQGIQETLIRRTSDHWPIALDTNPFMWGPTP 240

Query: 241 --------------------------------------EFENSRIKECNK----VIEEVL 300
                                                 +F  ++ KE NK    V+ E  
Sbjct: 241 FRFENMWLQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKK 300

Query: 301 KIALPDIG--------WAKEERLLRELEIIDGFAESVGLNE------------------- 360
           K  L D+               LL +  +  G  E + L E                   
Sbjct: 301 KSILKDLANLDAIEQDGGLTSELLGQRALRKGELEELILREEIHWRQKARVKWVKEGDCN 360

Query: 361 VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
            +  HK  N ++ +  I  L +E G++  + + I   IL ++E LY   +G  +     D
Sbjct: 361 SKFFHKVANGRRNRKYIKSLENETGLVLNNAVSITEEILLYFEKLYANPIGESWSIEGLD 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  +S     SL+  F+  EI KAI  +  +KAP PDGFT   F   W V+KE L+R+F 
Sbjct: 421 WSPISEESAISLVAPFTEEEISKAIFQMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFA 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 496
           EFHR+G +N     +FI L+ KK     + DFR ISL T  YK++AKVL+ RL+GV+   
Sbjct: 481 EFHRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHET 540

BLAST of CSPI05G13600 vs. NCBI nr
Match: RVW64408.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 310.1 bits (793), Expect = 3.5e-80
Identity = 188/594 (31.65%), Postives = 283/594 (47.64%), Query Frame = 0

Query: 1    MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
            MKI+SWNTRGL    KR  +++F+   +PD+V++QE+K E  +  F+ ++W    + W +
Sbjct: 736  MKILSWNTRGLGSKKKRRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKGKRVEWAA 795

Query: 61   VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
            + + GASGGI+ LWD SK+   E +   +S+++K  +  +   W+T+VYGP     RK  
Sbjct: 796  LPACGASGGIVILWDSSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDF 855

Query: 121  WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
            W EL  +       W +GGDFN+ R + E+    R T  MR F+ FI  + L++  L+N 
Sbjct: 856  WLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNA 915

Query: 181  KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
             FTWS          LDRF  +S+WD                                  
Sbjct: 916  AFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTP 975

Query: 241  -EFEN-------------------------------------SRIKECNKV----IEEVL 300
              FEN                                     S++KE N +    ++E  
Sbjct: 976  FRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERK 1035

Query: 301  KIALPDIGWAK-------------EERLLRELEIIDGFAE--------------SVGLNE 360
            K+ L D+                  ER L+  E+ D   +                G   
Sbjct: 1036 KLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELEDVLLKEEVQWRQKSRVKWIKEGDCN 1095

Query: 361  VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
             +  H+    ++ +  I  L+ E+G    +  DI   I+ F+ +LY K +G  +     D
Sbjct: 1096 SKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFGNLYSKPVGESWRVEGID 1155

Query: 421  WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
            W  +S      L   F+  E+ +A+  L   KAP PDGFT   + + W V+KE LMR+F 
Sbjct: 1156 WVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPGPDGFTIAVYQECWDVIKEDLMRVFL 1215

Query: 481  EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 493
            EFH NG +N      FI L+ KK  ++ + D+R ISL T  YK++AKVL+ RL+ V+   
Sbjct: 1216 EFHTNGVINQSTNATFIALVPKKSQSVKISDYRPISLVTSLYKIIAKVLSGRLRKVLHET 1275

BLAST of CSPI05G13600 vs. NCBI nr
Match: RVW16209.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 309.3 bits (791), Expect = 6.0e-80
Identity = 189/567 (33.33%), Postives = 279/567 (49.21%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F+++ +PD+V+IQE+K E  +  F+ ++W+  +  W +
Sbjct: 51  MKIISWNVRGLGSRNKRRMIKDFLRSENPDVVMIQETKKENCDRRFVGSVWTVRNKDWVA 110

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD   ++  E +   +S+S+K         W++ VYGP     RK  
Sbjct: 111 LPASGASGGILIIWDSKILSREEVVIGSFSVSVKFSLDGCGPLWISAVYGPNSPSLRKDF 170

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+      T  MR F++FI    L++  L+N 
Sbjct: 171 WVELFDIYGLTYPLWCVGGDFNVIRGSSEKMGGSSLTPSMRDFDSFISECELLDPPLRNA 230

Query: 181 KFTWSREDRSAARSLLDRFFINSKW----------------------------------D 240
            FTWS    S     LDRF  +++W                                   
Sbjct: 231 SFTWSNIQESPVCKRLDRFLYSNEWGLLFPQGLQEALIRRTSDHWPIVMDTNPFMWGPTP 290

Query: 241 DEFENSRIKECNKVIEEVLK---IALPDIGWAKEERLLRELEII---------DGFAESV 300
             FEN  ++  N   +E  +        IGW +  + +R L+ +           F E  
Sbjct: 291 FRFENMWLQHTN--FKENFRDWWSGFQGIGW-EGHKFMRRLQYVKAKLKEWNKSSFGELK 350

Query: 301 GLNEVELA-----------------------------HKFLNAKKRKNLITKLVDEQGVI 360
              + EL                              HK  N ++ +  I +L +E+G++
Sbjct: 351 EKKKRELEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVANGRRNRKYIKELENERGLV 410

Query: 361 TKSFLDIERVILWFYEDLYKKILGVRYLPFIADWPCVSVSQKFSLINSFSAVEIFKAIKA 420
            K+   I   IL ++E LY    G  +     DW  +S      L + F+  EI KAI  
Sbjct: 411 LKNAESITEEILHYFEKLYTNPTGESWGVEGLDWSPISEESALRLESPFTEEEISKAIFQ 470

Query: 421 LGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFEEFHRNGKLNACIQENFICLIQKKEDAI 480
           L  +KAP PDGFT   F + W V+KE L+R+F EFHR+G +N     +FI LI KK  + 
Sbjct: 471 LDRDKAPGPDGFTIAVFQECWDVIKEDLVRVFAEFHRSGIINQSTNASFIVLIPKKSLSK 530

Query: 481 HVRDFRLISLTTITYKVVAKVLADRLKGVMDSIISPFQSAFIEGRQVLDPILIANEVVED 493
            + DFR ISL T  YK++AKVL+ RL+GV+   I   Q AF++GRQ+LD +LIANE+V++
Sbjct: 531 RISDFRPISLITSLYKIIAKVLSGRLRGVLHETIHYTQGAFVQGRQILDAVLIANEIVDE 590

BLAST of CSPI05G13600 vs. NCBI nr
Match: RVW43689.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 306.6 bits (784), Expect = 3.9e-79
Identity = 191/597 (31.99%), Postives = 278/597 (46.57%), Query Frame = 0

Query: 1   MKIISWNTRGLKDPNKRSALKKFIKNHHPDMVLIQESKMEILEVNFIKTIWSSMDIGWES 60
           MKIISWN RGL   NKR  +K F+K   PD+V+IQE+K E  +   + ++W+  +  W  
Sbjct: 1   MKIISWNVRGLGSSNKRRVIKDFLKLEKPDVVMIQETKKEKCDRRLVGSVWTVRNKDWVF 60

Query: 61  VESYGASGGILTLWDKSKITVVETIRRHYSLSIKCVTLCKKCCWVTNVYGPCGYRERKLV 120
           + + GASGGIL +WD  K++  E +   +S+ +K         W++ VYGP     RK  
Sbjct: 61  LPACGASGGILFIWDSKKLSKEEVVLGSFSILVKFALEGCGPLWISAVYGPNSPSLRKDF 120

Query: 121 WPELLTIAECGEEAWSLGGDFNITRWVYERFPVGRSTRGMRQFNAFIDSANLMEISLQNG 180
           W EL  I       W +GGDFN+ R   E+    R T  MR F++FI  + L++  L+N 
Sbjct: 121 WVELYDICGLTFPLWCVGGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNA 180

Query: 181 KFTWSREDRSAARSLLDRFFINSKWDD--------------------------------- 240
            FTWS    S     LD F  +++W                                   
Sbjct: 181 SFTWSNMQESPVCKRLDHFLYSNEWGQLFPQGIQETLIRRTSDHWPIALDTNPFTWGPTP 240

Query: 241 --------------------------------------EFENSRIKECNK----VIEEVL 300
                                                 +F  ++ KE NK    V+ E  
Sbjct: 241 FRFENMWMQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKK 300

Query: 301 KIALPDI----------GWAKE---ERLLRELEIIDGFAE--------------SVGLNE 360
           K  L D+          G   E   +R LR+ E+ D                    G   
Sbjct: 301 KSILKDLANLDAIEQDGGLTSELLGQRALRKGELEDLILREEIHWRQKARVKWVKEGDCN 360

Query: 361 VELAHKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLYKKILGVRYLPFIAD 420
            +  HK  N ++ +  I  L +E G++  +   I   IL ++E LY   +G  +     D
Sbjct: 361 SKFFHKVANGRRNRKYIKSLENETGLVLNNAASITEEILLYFEKLYANPIGESWSIEGLD 420

Query: 421 WPCVSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMRLFE 480
           W  +S     SL+  F+  EI KAI  +  +KAP PDGFT   F   W V+KE L+R+F 
Sbjct: 421 WSPISEESAISLVAPFTEEEISKAIFQMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFA 480

Query: 481 EFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVVAKVLADRLKGVMDSI 496
           EFHR+G +N     +FI L+ KK     + DFR ISL T  YK++AKVL+ RL+GV+   
Sbjct: 481 EFHRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHET 540

BLAST of CSPI05G13600 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.3e-11
Identity = 52/168 (30.95%), Postives = 82/168 (48.81%), Query Frame = 0

Query: 263 HKFLNAKKRKNLITKLVDEQGVITKSFLDIERVILWFYEDLY---KKIL---GVRYLPFI 322
           HK + A + KNLI  L  +  V  ++   ++ +I+ +Y  L      IL    V+ +  I
Sbjct: 455 HKVILANQAKNLIKFLRMDDDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDI 514

Query: 323 ADWPC-VSVSQKFSLINSFSAVEIFKAIKALGGNKAPRPDGFTTEFFVKHWPVLKEGLMR 382
             + C  +++ + S + S    EI  A+ A+  NKAP PD FT EFF + W V+K+  + 
Sbjct: 515 HPFRCNDTLASRLSALPSDK--EITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIA 574

Query: 383 LFEEFHRNGKLNACIQENFICLIQKKEDAIHVRDFRLISLTTITYKVV 424
             +EF R G L        I LI K      +  FR +S  T+ YK++
Sbjct: 575 AVKEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

BLAST of CSPI05G13600 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 64.3 bits (155), Expect = 3.1e-10
Identity = 32/68 (47.06%), Postives = 45/68 (66.18%), Query Frame = 0

Query: 427 LADRLKGVMDSIISPFQSAFIEGRQVLDPILIANEVVEDY-QAKGKKGW-ILKLDLEKAF 486
           + +RLK +M ++I P Q++FI GR   D I+   E V    + KG KGW +LKLDLEKA+
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 487 DRVDWGFL 493
           DR+ W +L
Sbjct: 61  DRIRWDYL 68

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P143811.3e-2133.33Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
O003705.0e-2128.53LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P113695.6e-2030.60LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085481.2e-1728.52LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
A0A803P8A02.1e-8332.32Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QEA65.3e-8231.53Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A438CWL63.4e-8132.16Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX... [more]
A0A438KGJ27.6e-8131.83Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX... [more]
A0A438FWU51.7e-8031.65LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF... [more]
Match NameE-valueIdentityDescription
RVW27595.17.1e-8132.16Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera][more]
RVX20328.11.6e-8031.83Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera][more]
RVW64408.13.5e-8031.65LINE-1 retrotransposable element ORF2 protein [Vitis vinifera][more]
RVW16209.16.0e-8033.33Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera][more]
RVW43689.13.9e-7931.99Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera][more]
Match NameE-valueIdentityDescription
AT1G43760.11.3e-1130.95DNAse I-like superfamily protein [more]
AT4G20520.13.1e-1047.06RNA binding;RNA-directed DNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 1..222
e-value: 1.6E-30
score: 108.6
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 1..215
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 399..494
e-value: 4.2E-18
score: 65.7
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 235..495
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 235..495
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 395..495
e-value: 3.53121E-29
score: 112.385

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G13600.1CSPI05G13600.1mRNA