CSPI05G13160 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G13160 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 368/550 (66.91%), Postives = 461/550 (83.82%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPA+S PLTP+GF+ERAA +YGD TS++Y++ +YTWS+THRRCL LASSI+S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+ GHVVSV APN P MYELHFAVPMAGAILN +NLRLD RTISILL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI +A+ALFP     P LV + D++   +SS      +   F  SY++++  GD  F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADESESGNSS-----ELGKEFFCSYKDLIDRGDPDF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           +W+ P SEWDP++LNYTSGTTS+PKGVV+CHRG+F + +DS+++WGVPK+ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWS+PWG+AAVGGTN+C+RKFDS +I+ +I+RH VTH+CGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
              V+I+TAGAPPP++VL RTESLGF V+HGYGLTE+AG ++ C+WK EWN LPA E+AR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LK+RQGV  +    +DV+DP T  +VKRDG+++GE+V+RG S+MLGYLKD E T+K+M+ 
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + EAAVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAF+SLK+GL++KPTE+EI+EYC+ KLP++MVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540

Query: 541 VLRQKAKSLG 551
           +LR  A+ +G
Sbjct: 541 ILRDMARGMG 545

BLAST of CSPI05G13160 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 2.2e-219
Identity = 356/552 (64.49%), Postives = 453/552 (82.07%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISS-F 60
           ME+LKPR ANS PLTP+GF+ERAA +YGD  S++Y+  +YTWS+TH RCL++AS I S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           G+++G VVSV APN PAMYEL+FAVPMAG +LN IN RL+  TIS++LRHSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L+ DA++LFP +   PLLVLI D+   ++ +    V  R++FV  YE++V+ GD K
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLT----VEDREHFVCCYEDLVEKGDDK 180

Query: 181 -FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLP 240
            F+W+RP+SEWDPIVLNYTSGTTS+PKGVV+ HR VF V LDS+++WGVPK+ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240

Query: 241 MFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNL 300
           MFH NGW + WGIAAVGGTN+C+R+FD  +IF+LIRRHRVTH+C AP+VLNML+NSP+  
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV ++T GAPPPA+VLLRTESLGFV++HGYG+TE  G ++ C+WK EWN+LPA EQ
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           ARLK+RQGVR  A+  VDV+DP +  SVKRDG ++GEIV++G S+MLGYLK++ AT+K +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
             DGWF+TGD+ VMHPDGY+EIKDRSKDVIISGGEN+SSVEVES LY +P V+EAAVVA 
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480

Query: 481 PDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDE WGETP AF++LK+G+  +PTE+EI+EYC+ KL  FMVPK V+F  ++PKT+TGK Q
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540

Query: 541 KFVLRQKAKSLG 551
           KF L++  K++G
Sbjct: 541 KFTLKEIVKTMG 548

BLAST of CSPI05G13160 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 4.4e-215
Identity = 358/558 (64.16%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D  WGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CSPI05G13160 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 4.8e-214
Identity = 356/551 (64.61%), Postives = 448/551 (81.31%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DE WGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CSPI05G13160 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.9e-202
Identity = 341/551 (61.89%), Postives = 434/551 (78.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+     + V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDE WGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of CSPI05G13160 vs. ExPASy TrEMBL
Match: A0A0A0KMT3 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 554/557 (99.46%), Postives = 555/557 (99.64%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRH VTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CSPI05G13160 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.1e-309
Identity = 524/557 (94.08%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRH VTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CSPI05G13160 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.1e-309
Identity = 524/557 (94.08%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRH VTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CSPI05G13160 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.9e-268
Identity = 447/557 (80.25%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S V  R +FVDSYE MV+ GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+ VTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CSPI05G13160 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.5e-266
Identity = 447/561 (79.68%), Postives = 498/561 (88.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNTPAMYELHFAVPMAGA+LNTINLRLD+RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQD----SSLPSVVSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +D    SS  S VS R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWT 240
           D  FQWIRP+SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGV K+SVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+ VTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDE WGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 558
            QKFVLR+ AKS+GP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CSPI05G13160 vs. NCBI nr
Match: XP_004148908.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hypothetical protein Csa_023091 [Cucumis sativus])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 554/557 (99.46%), Postives = 555/557 (99.64%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRH VTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CSPI05G13160 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 524/557 (94.08%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRH VTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CSPI05G13160 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 932.6 bits (2409), Expect = 1.6e-267
Identity = 447/557 (80.25%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S V  R +FVDSYE MV+ GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+ VTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 ELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E WGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CSPI05G13160 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 928.3 bits (2398), Expect = 3.1e-266
Identity = 447/561 (79.68%), Postives = 498/561 (88.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNTPAMYELHFAVPMAGA+LNTINLRLD+RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQD----SSLPSVVSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +D    SS  S VS R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWT 240
           D  FQWIRP+SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGV K+SVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+ VTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDE WGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 558
            QKFVLR+ AKS+GP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CSPI05G13160 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.1 bits (2387), Expect = 5.8e-265
Identity = 445/561 (79.32%), Postives = 495/561 (88.24%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIY+STTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQD----SSLPSVVSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +D    SS  S V  R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWT 240
           D  FQWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+ VTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           ++ARLKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDE WGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 558
            QKFVLR+ AKS+GP  KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of CSPI05G13160 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 748.8 bits (1932), Expect = 3.1e-216
Identity = 358/558 (64.16%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D  WGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CSPI05G13160 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 745.3 bits (1923), Expect = 3.4e-215
Identity = 356/551 (64.61%), Postives = 448/551 (81.31%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DE WGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CSPI05G13160 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-203
Identity = 341/551 (61.89%), Postives = 434/551 (78.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+     + V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDE WGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of CSPI05G13160 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 687.6 bits (1773), Expect = 8.5e-198
Identity = 329/555 (59.28%), Postives = 428/555 (77.12%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +H VTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDELWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD++WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CSPI05G13160 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.6e-196
Identity = 320/551 (58.08%), Postives = 423/551 (76.77%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSIS--S 60
           ME L P  +NS PLT +GF+ERAA ++GDS S+++ +T +TWSETH RCL++AS++S  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  FGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVD 120
            GI RG VVSV  PN P++YEL FAVPM+GA+LN IN RLD   +S+LLRHSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDS 180
             S +L+ +A++  P D +RP LV++ D      + +PS  S   +F+D+YE  ++ GD 
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILND-----GNDMPSSSSADMDFLDTYEGFMERGDL 180

Query: 181 KFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKKSVYLWTLP 240
           +F+W+RP SEW P+VLNYTSGTTS+PKGVV+ HR VF   ++S+L+W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHRVTHLCGAPVVLNMLTNSPDNL 300
           MFH NGWS+ W  AAVG  N+C+ + D   IF+LI +++VTH+C AP+VLNMLTN P   
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PVK++TAGAPPPA+V+ + E+LGF V+HGYG+TE+ G ++ C+ K EW++L   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+ K+RQG+R      VDV DP + KSVK DGA++GEIV RG S+MLGY KD E T+ +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            EDGWF+TGD+GVMHPDGY+E+KDRSKDV+I GGEN+SS E+E+VLY NP + EAAVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDELWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PD++WGETPCAF+SLK       TE EI E+CK KLPK+MVP+ V+F+ E+PKT+TGK Q
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KFVLRQKAKSL 550
           KF+LRQ AKSL
Sbjct: 541 KFLLRQMAKSL 544