CSPI05G13000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G13000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Thiamine-phosphate pyrophosphorylase
Location	Chr5: 12494285 .. 12494941 (+)
RNA-Seq Expression	CSPI05G13000
Synteny	CSPI05G13000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCATGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTGATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGAAATGTTTGAATAATTTTGTAATGCTTTGCTTTTTGTAGAAAGCCTTTTGAAATAAAAATAATTGTCACAATATATATGTCATGTAAGATTCTATTCATATAAAGTTGAGCATATTTATATTCGCGA

mRNA sequence

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCATGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTGATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGAAATGTTTGAATAATTTTGTAATGCTTTGCTTTTTGTAGAAAGCCTTTTGAAATAAAAATAATTGTCACAATATATATGTCATGTAAGATTCTATTCATATAAAGTTGAGCATATTTATATTCGCGA

Coding sequence (CDS)

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCATGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTGATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

Protein sequence

MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT*

Homology

[Close]

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot
Match: Q5M731 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TH1 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 2.7e-70
Identity = 131/172 (76.16%), Postives = 146/172 (84.88%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI IC +HGV LLINDRIDIALACDADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGLK VC ASKLP
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

[Close]

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot
Match: O48881 (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus OX=3708 GN=BTH1 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.6e-68
Identity = 127/172 (73.84%), Postives = 146/172 (84.88%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AKSC+ IC ++GV LLINDR DIA+A DADGVHVGQSD+P   VR L
Sbjct: 348 REKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALDADGVHVGQSDMPVDLVRSL 407

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGL+ VC ASKLP
Sbjct: 408 LGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLREVCKASKLP 467

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM IG PNLKGVAVVSALFD++CVL +A KLH TL E+
Sbjct: 468 VVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQAKKLHKTLTES 519

[Close]

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot
Match: Q2QWK9 (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 2.6e-68
Identity = 124/173 (71.68%), Postives = 146/173 (84.39%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKD++TR+FLE AK+C++IC + GVPLLINDR+DIALAC+ADGVHVGQ D+ AHEVR L
Sbjct: 374 REKDSETREFLEAAKACMEICKSSGVPLLINDRVDIALACNADGVHVGQLDMSAHEVREL 433

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT  QA+QAW DGADYIGCGGV+PT+TKANN T+G DGLK VCLASKLP
Sbjct: 434 LGPGKIIGVSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLP 493

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 176
           VVAIGGIN +NA +VM +G+PNLKGVAVVSALFDR  V+ E   + + L   +
Sbjct: 494 VVAIGGINASNAGSVMELGLPNLKGVAVVSALFDRPSVVAETRNMKSILTNTS 546

[Close]

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot
Match: A8MK92 (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=thiE PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.0e-37
Identity = 80/169 (47.34%), Postives = 113/169 (66.86%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REK+A + +F ++A     +   + VPL+INDR+DIALA DADGVHVGQSD+PAH VR +
Sbjct: 43  REKEASSLEFYQLALKAKALTEKYNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSM 102

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           +G NKI+GVS  T E++++A  DGADYIG G ++PT TK +   V +D L+ +     +P
Sbjct: 103 IGQNKILGVSTATLEESKKAAEDGADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIP 162

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATL 172
           VV IGGI   N   +M +GI    GVA+VSA+  ++ + E A  L A++
Sbjct: 163 VVGIGGICEDNIKTIMEVGI---DGVAIVSAILGKENIKEAAESLKASI 208

[Close]

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot
Match: Q893R0 (Thiamine-phosphate synthase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=thiE PE=3 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 4.9e-35
Identity = 71/165 (43.03%), Postives = 111/165 (67.27%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REK+  +++F E+AK    I   + +PL+INDR+DIALA +A+G+H+GQ D+PA+ VR++
Sbjct: 42  REKNVSSKEFYEIAKDIKVITDKYNIPLIINDRVDIALAVNAEGIHIGQKDLPANIVRKI 101

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           +G +KI+GVS  T E A +A  DGADY+G G ++PTN+K +  +  I+ LK +  A  +P
Sbjct: 102 IGEDKILGVSANTIEDALKAQRDGADYLGVGAIFPTNSKKDAESTSIETLKEIKNAVNIP 161

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKL 168
           +VAIGGIN  N   V  +   N+ G+AV+S +  ++ V +   +L
Sbjct: 162 IVAIGGINENN---VQKLKETNIDGIAVISTILGKEDVKKACEEL 203

[Close]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL
Match: A0A0A0KPF9 (Thiamine phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G262270 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.3e-94
Identity = 174/176 (98.86%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120
           RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK
Sbjct: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 176

[Close]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL
Match: A0A5D3E4F9 (Thiamine phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold349G00520 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.4e-93
Identity = 172/176 (97.73%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 1   MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120
           RLLGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK
Sbjct: 61  RLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           LPVVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 176

[Close]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL
Match: A0A1S3CCI6 (Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.6e-92
Identity = 170/174 (97.70%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 419 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532

[Close]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL
Match: A0A1S3CDB7 (Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.6e-92
Identity = 170/174 (97.70%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL
Sbjct: 339 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 398

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 399 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 459 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 512

[Close]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL
Match: A0A6J1GZX4 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.0e-84
Identity = 154/173 (89.02%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLE AKSCI+ICH HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R L
Sbjct: 339 REKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSL 398

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVG+DGLKRVCLASKLP
Sbjct: 399 LGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 176
           VVAIGGINH+NAAAVM IG+PNLKGVAVVSALFDRQCVLEE  KLHATLVEAT
Sbjct: 459 VVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEAT 511

[Close]

BLAST of CSPI05G13000 vs. NCBI nr
Match: KAA0035963.1 (thiamine biosynthetic bifunctional enzyme TH1 [Cucumis melo var. makuwa] >TYK30440.1 thiamine biosynthetic bifunctional enzyme TH1 [Cucumis melo var. makuwa])

HSP 1 Score: 352.1 bits (902), Expect = 2.9e-93
Identity = 172/176 (97.73%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 1   MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120
           RLLGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK
Sbjct: 61  RLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           LPVVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 176

[Close]

BLAST of CSPI05G13000 vs. NCBI nr
Match: KAE8648089.1 (hypothetical protein Csa_004689 [Cucumis sativus])

HSP 1 Score: 349.0 bits (894), Expect = 2.4e-92
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 347 REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 406

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 407 LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 467 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 520

[Close]

BLAST of CSPI05G13000 vs. NCBI nr
Match: XP_031741473.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 349.0 bits (894), Expect = 2.4e-92
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 345 REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 404

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 405 LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 464

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 465 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 518

[Close]

BLAST of CSPI05G13000 vs. NCBI nr
Match: XP_004140412.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 349.0 bits (894), Expect = 2.4e-92
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 419 LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 532

[Close]

BLAST of CSPI05G13000 vs. NCBI nr
Match: XP_008460244.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 347.8 bits (891), Expect = 5.4e-92
Identity = 170/174 (97.70%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL
Sbjct: 339 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 398

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 399 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 459 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 512

[Close]

BLAST of CSPI05G13000 vs. TAIR 10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative )

HSP 1 Score: 266.2 bits (679), Expect = 1.9e-71
Identity = 131/172 (76.16%), Postives = 146/172 (84.88%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI IC +HGV LLINDRIDIALACDADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGLK VC ASKLP
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

The following BLAST results are available for this feature:

BLAST of CSPI05G13000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5M731	2.7e-70	76.16	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
O48881	2.6e-68	73.84	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
Q2QWK9	2.6e-68	71.68	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
A8MK92	4.0e-37	47.34	Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688...	[more]
Q893R0	4.9e-35	43.03	Thiamine-phosphate synthase OS=Clostridium tetani (strain Massachusetts / E88) O...	[more]

BLAST of CSPI05G13000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPF9	6.3e-94	98.86	Thiamine phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G262270 PE=3 SV=1	[more]
A0A5D3E4F9	1.4e-93	97.73	Thiamine phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3CCI6	2.6e-92	97.70	Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=...	[more]
A0A1S3CDB7	2.6e-92	97.70	Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=...	[more]
A0A6J1GZX4	2.0e-84	89.02	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]

BLAST of CSPI05G13000 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0035963.1	2.9e-93	97.73	thiamine biosynthetic bifunctional enzyme TH1 [Cucumis melo var. makuwa] >TYK304...	[more]
KAE8648089.1	2.4e-92	98.85	hypothetical protein Csa_004689 [Cucumis sativus]	[more]
XP_031741473.1	2.4e-92	98.85	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis...	[more]
XP_004140412.1	2.4e-92	98.85	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis...	[more]
XP_008460244.1	5.4e-92	97.70	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]

BLAST of CSPI05G13000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22940.1	1.9e-71	76.16	thiamin biosynthesis protein, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022998	Thiamine phosphate synthase/TenI	PFAM	PF02581	TMP-TENI	coord: 3..154 e-value: 5.6E-55 score: 185.4
IPR022998	Thiamine phosphate synthase/TenI	CDD	cd00564	TMP_TenI	coord: 3..169 e-value: 9.90505E-72 score: 213.149
IPR034291	Thiamine phosphate synthase	TIGRFAM	TIGR00693	TIGR00693	coord: 3..162 e-value: 2.1E-53 score: 178.5
IPR034291	Thiamine phosphate synthase	HAMAP	MF_00097	TMP_synthase	coord: 2..173 score: 19.560806
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..175 e-value: 6.5E-62 score: 210.3
None	No IPR available	PANTHER	PTHR20857	THIAMINE-PHOSPHATE PYROPHOSPHORYLASE	coord: 3..172
IPR036206	Thiamin phosphate synthase superfamily	SUPERFAMILY	51391	Thiamin phosphate synthase	coord: 2..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G13000.1	CSPI05G13000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity
molecular_function	GO:0003824	catalytic activity