CSPI05G12450 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G12450
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr5: 11671668 .. 11672435 (-)
RNA-Seq Expression	CSPI05G12450
Synteny	CSPI05G12450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAATGCAAAATCAGTTAGTTCCCCTTTACCTAGTCACTTCAAACTAATTAGTAAACAGAGCCCTTCTACGGATAAAGAGAAGGAGGATATGAGTAAGGTTCTGTATGCTTCAGCAGTTGGAAGCCTAATGTATGTCATGATATGTACTAGACCTGATATTGCTCATGTTATTGGTGTTAGTCATTTTATGTCTAATCTAGGAAAACAACATTGGGAGGCAGTCAAGTGGATCATGAGATATTTGAGATGTACTTCCAGTTTGAAACTCACATTTGGGGATGAAAAGTCAGTACTTGTTGGATATACTGATTCAGATATGACAGTGGACTTAGACAGCAGAAAGTCTACTTCTGGTTACTTGATGACATTTGCAGGTGGTGCAGTGTCCTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTAAAGCAGAGTATATTGCAGTAGCAGAAGCATATAAAGAGATGTTATGGATGAAGTGCTTTGTACAAGAGCTTGGCTTCAAGCAACAACGGTATGTGATATACTATGACAATCAGAGTGTTCACCTTGGTAAGAATGATTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCACTGGCTTAGAGATGCTTTAAATGATAAATTATTTGAGCTCGAGAAGATACATACTGATCATAATGGATCTGATATGTTGACAAAAAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAGTGA

mRNA sequence

ATGAGTAATGCAAAATCAGTTAGTTCCCCTTTACCTAGTCACTTCAAACTAATTAGTAAACAGAGCCCTTCTACGGATAAAGAGAAGGAGGATATGAGTAAGGTTCTGTATGCTTCAGCAGTTGGAAGCCTAATGTATGTCATGATATGTACTAGACCTGATATTGCTCATGTTATTGGTGTTAGTCATTTTATGTCTAATCTAGGAAAACAACATTGGGAGGCAGTCAAGTGGATCATGAGATATTTGAGATGTACTTCCAGTTTGAAACTCACATTTGGGGATGAAAAGTCAGTACTTGTTGGATATACTGATTCAGATATGACAGTGGACTTAGACAGCAGAAAGTCTACTTCTGGTTACTTGATGACATTTGCAGGTGGTGCAGTGTCCTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTAAAGCAGAGTATATTGCAGTAGCAGAAGCATATAAAGAGATGTTATGGATGAAGTGCTTTGTACAAGAGCTTGGCTTCAAGCAACAACGGTATGTGATATACTATGACAATCAGAGTGTTCACCTTGGTAAGAATGATTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCACTGGCTTAGAGATGCTTTAAATGATAAATTATTTGAGCTCGAGAAGATACATACTGATCATAATGGATCTGATATGTTGACAAAAAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAGTGA

Coding sequence (CDS)

ATGAGTAATGCAAAATCAGTTAGTTCCCCTTTACCTAGTCACTTCAAACTAATTAGTAAACAGAGCCCTTCTACGGATAAAGAGAAGGAGGATATGAGTAAGGTTCTGTATGCTTCAGCAGTTGGAAGCCTAATGTATGTCATGATATGTACTAGACCTGATATTGCTCATGTTATTGGTGTTAGTCATTTTATGTCTAATCTAGGAAAACAACATTGGGAGGCAGTCAAGTGGATCATGAGATATTTGAGATGTACTTCCAGTTTGAAACTCACATTTGGGGATGAAAAGTCAGTACTTGTTGGATATACTGATTCAGATATGACAGTGGACTTAGACAGCAGAAAGTCTACTTCTGGTTACTTGATGACATTTGCAGGTGGTGCAGTGTCCTGGCAGTCAAGGTTGCAGAAATGTGTTGCCCTTTCTACAACTAAAGCAGAGTATATTGCAGTAGCAGAAGCATATAAAGAGATGTTATGGATGAAGTGCTTTGTACAAGAGCTTGGCTTCAAGCAACAACGGTATGTGATATACTATGACAATCAGAGTGTTCACCTTGGTAAGAATGATTCATTTCATTCCAGAACAAAGCATATTGATGTGAGATATCACTGGCTTAGAGATGCTTTAAATGATAAATTATTTGAGCTCGAGAAGATACATACTGATCATAATGGATCTGATATGTTGACAAAAAATCTACCAAGATCGAAGCTTGAGTTTTGTCGTTCCACAGTGGGAATGACAAATTCATCCTCAAAGTGA

Protein sequence

MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIGVSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTSGYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIYYDNQSVHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRSKLEFCRSTVGMTNSSSK*

Homology

[Close]

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.4e-83
Identity = 151/251 (60.16%), Postives = 194/251 (77.29%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            M NAK VS+PL  H KL  K  P+T +EK +M+KV Y+SAVGSLMY M+CTRPDIAH +G
Sbjct: 1075 MKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKVPYSSAVGSLMYAMVCTRPDIAHAVG 1134

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+ N GK+HWEAVKWI+RYLR T+   L FG    +L GYTD+DM  D+D+RKS++
Sbjct: 1135 VVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILKGYTDADMAGDIDNRKSST 1194

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYL TF+GGA+SWQS+LQKCVALSTT+AEYIA  E  KEM+W+K F+QELG  Q+ YV+Y
Sbjct: 1195 GYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGLHQKEYVVY 1254

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             D+QS + L KN  +H+RTKHIDVRYHW+R+ ++D+  ++ KI T+ N +DMLTK +PR+
Sbjct: 1255 CDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVPRN 1314

Query: 241  KLEFCRSTVGM 250
            K E C+  VGM
Sbjct: 1315 KFELCKELVGM 1325

[Close]

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 150.2 bits (378), Expect = 3.2e-35
Identity = 89/256 (34.77%), Postives = 147/256 (57.42%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            M N  +VS+PLPS    I+ +  ++D++     +    S +G LMY+M+CTRPD+   + 
Sbjct: 1152 MENCNAVSTPLPSK---INYELLNSDEDCNTPCR----SLIGCLMYIMLCTRPDLTTAVN 1211

Query: 61   V-SHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDE---KSVLVGYTDSDMTVDLDSRK 120
            + S + S    + W+ +K ++RYL+ T  +KL F      ++ ++GY DSD       RK
Sbjct: 1212 ILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRK 1271

Query: 121  STSGYLM-TFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQR 180
            ST+GYL   F    + W ++ Q  VA S+T+AEY+A+ EA +E LW+K  +  +  K + 
Sbjct: 1272 STTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLEN 1331

Query: 181  YV-IYYDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTK 240
             + IY DNQ  + +  N S H R KHID++YH+ R+ + + +  LE I T++  +D+ TK
Sbjct: 1332 PIKIYEDNQGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTK 1391

Query: 241  NLPRSKLEFCRSTVGM 250
             LP ++    R  +G+
Sbjct: 1392 PLPAARFVELRDKLGL 1400

[Close]

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-27
Identity = 64/133 (48.12%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 32  MSKVLYASAVGSLMYVMICTRPDIAHVIGV-SHFMSNLGKQHWEAVKWIMRYLRCTSSLK 91
           M  V Y SAVG++MY+M+ TRPD+A  +GV S F S+    HW+A+K ++RYL+ T +  
Sbjct: 1   MKNVPYLSAVGAIMYLMVVTRPDLAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYG 60

Query: 92  LTFGDEKSV-LVGYTDSDMTVDLDSRKSTSGYLMTFAGGAVSWQSRLQKCVALSTTKAEY 151
           L F    +  LVGY+D+D   D++SR+STSGYL    GG VSW+S+ Q+ VALS+T+ EY
Sbjct: 61  LEFTRAGTAKLVGYSDADWAGDVESRRSTSGYLFKLNGGCVSWRSKKQRTVALSSTEDEY 120

Query: 152 IAVAEAYKEMLWM 163
           +A++EA +E +W+
Sbjct: 121 MALSEATQEAVWL 133

[Close]

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-26
Identity = 90/255 (35.29%), Postives = 133/255 (52.16%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            M  AK V++P+    KL            E      Y   VGSL Y+   TRPDI++ + 
Sbjct: 1215 MITAKPVTTPMAPSPKLSLYSGTKLTDPTE------YRGIVGSLQYLAF-TRPDISYAVN 1274

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSS--LKLTFGDEKSVLVGYTDSDMTVDLDSRKS 120
             +S FM    ++H +A+K I+RYL  T +  + L  G+  S L  Y+D+D   D D   S
Sbjct: 1275 RLSQFMHMPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLS-LHAYSDADWAGDKDDYVS 1334

Query: 121  TSGYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQR-Y 180
            T+GY++      +SW S+ QK V  S+T+AEY +VA    EM W+   + ELG +  R  
Sbjct: 1335 TNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPP 1394

Query: 181  VIYYDN-QSVHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNL 240
            VIY DN  + +L  N  FHSR KHI + YH++R+ +      +  + T    +D LTK L
Sbjct: 1395 VIYCDNVGATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPL 1454

Query: 241  PRSKLEFCRSTVGMT 251
             R+  +   S +G+T
Sbjct: 1455 SRTAFQNFASKIGVT 1461

[Close]

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 4.3e-24
Identity = 84/254 (33.07%), Postives = 127/254 (50.00%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            M  AK V++P+ +  KL            E      Y   VGSL Y+   TRPD+++ + 
Sbjct: 1198 MLTAKPVATPMATSPKLTLHSGTKLPDPTE------YRGIVGSLQYLAF-TRPDLSYAVN 1257

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCT--SSLKLTFGDEKSVLVGYTDSDMTVDLDSRKS 120
             +S +M      HW A+K ++RYL  T    + L  G+  S L  Y+D+D   D D   S
Sbjct: 1258 RLSQYMHMPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLS-LHAYSDADWAGDTDDYVS 1317

Query: 121  TSGYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFK-QQRY 180
            T+GY++      +SW S+ QK V  S+T+AEY +VA    E+ W+   + ELG +     
Sbjct: 1318 TNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSELQWICSLLTELGIQLSHPP 1377

Query: 181  VIYYDN-QSVHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNL 240
            VIY DN  + +L  N  FHSR KHI + YH++R+ +      +  + T    +D LTK L
Sbjct: 1378 VIYCDNVGATYLCANPVFHSRMKHIALDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPL 1437

Query: 241  PRSKLEFCRSTVGM 250
             R   +     +G+
Sbjct: 1438 SRVAFQNFSRKIGV 1443

[Close]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL
Match: A0A438CN91 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3654 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.5e-106
Identity = 195/257 (75.88%), Postives = 222/257 (86.38%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMY M+CTRPDIA+ +G
Sbjct: 962  MSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAVG 1021

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 1022 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 1081

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1082 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 1141

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1142 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1201

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+S+
Sbjct: 1202 KLGVCCSIAGMISSNSR 1218

[Close]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL
Match: A0A438HN89 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3233 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.0e-105
Identity = 194/257 (75.49%), Postives = 221/257 (85.99%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMYVM+CTRPDIA+ +G
Sbjct: 1071 MSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYVMVCTRPDIAYSVG 1130

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 1131 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 1190

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYL TF+GGAVSWQSRLQKCV LSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1191 GYLTTFSGGAVSWQSRLQKCVVLSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 1250

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1251 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1310

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S VGM +S+ +
Sbjct: 1311 KLGVCCSIVGMISSNGR 1327

[Close]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL
Match: A0A438CLF9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2520 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.0e-105
Identity = 193/257 (75.10%), Postives = 220/257 (85.60%), Query Frame = 0


Query: 1   MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
           M  AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMY M+CTRPDIA+ +G
Sbjct: 322 MGKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAVG 381

Query: 61  -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
            VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 382 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 441

Query: 121 GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
           GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 442 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 501

Query: 181 YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
            DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 502 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 561

Query: 241 KLEFCRSTVGMTNSSSK 256
           KL  C S  GM +S+ +
Sbjct: 562 KLGVCCSIAGMISSNGR 578

[Close]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL
Match: A5C9D7 (Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_007304 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.7e-105
Identity = 192/257 (74.71%), Postives = 219/257 (85.21%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS  K VSSPL SHFKL S+ SPSTDKEKEDM +V YASA+GSLMY M+CTRPDIA+ +G
Sbjct: 1046 MSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRPDIAYAVG 1105

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+RKSTS
Sbjct: 1106 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRKSTS 1165

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA  EA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1166 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFKQQRYVVY 1225

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1226 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1285

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+ +
Sbjct: 1286 KLGVCCSIAGMISSNGR 1302

[Close]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL
Match: A0A438GP95 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2090 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.7e-105
Identity = 192/257 (74.71%), Postives = 219/257 (85.21%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS  K VSSPL SHFKL S+ SPSTDKEKEDM +V YASA+GSLMY M+CTRPDIA+ +G
Sbjct: 992  MSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRPDIAYAVG 1051

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+RKSTS
Sbjct: 1052 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRKSTS 1111

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA  EA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1112 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFKQQRYVVY 1171

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1172 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1231

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+ +
Sbjct: 1232 KLGVCCSIAGMISSNGR 1248

[Close]

BLAST of CSPI05G12450 vs. NCBI nr
Match: RVW24680.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 394.4 bits (1012), Expect = 7.3e-106
Identity = 195/257 (75.88%), Postives = 222/257 (86.38%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMY M+CTRPDIA+ +G
Sbjct: 962  MSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAVG 1021

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 1022 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 1081

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1082 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 1141

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1142 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1201

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+S+
Sbjct: 1202 KLGVCCSIAGMISSNSR 1218

[Close]

BLAST of CSPI05G12450 vs. NCBI nr
Match: RVW85908.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 391.3 bits (1004), Expect = 6.2e-105
Identity = 194/257 (75.49%), Postives = 221/257 (85.99%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMYVM+CTRPDIA+ +G
Sbjct: 1071 MSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYVMVCTRPDIAYSVG 1130

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 1131 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 1190

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYL TF+GGAVSWQSRLQKCV LSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1191 GYLTTFSGGAVSWQSRLQKCVVLSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 1250

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1251 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1310

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S VGM +S+ +
Sbjct: 1311 KLGVCCSIVGMISSNGR 1327

[Close]

BLAST of CSPI05G12450 vs. NCBI nr
Match: RVW24009.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 391.3 bits (1004), Expect = 6.2e-105
Identity = 193/257 (75.10%), Postives = 220/257 (85.60%), Query Frame = 0


Query: 1   MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
           M  AK VSSPL SHFKL S+ SPSTDKEKEDM +V YASAVGSLMY M+CTRPDIA+ +G
Sbjct: 322 MGKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLMYAMVCTRPDIAYAVG 381

Query: 61  -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
            VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+R+STS
Sbjct: 382 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRRSTS 441

Query: 121 GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
           GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA AEA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 442 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKCFMQELGFKQQRYVVY 501

Query: 181 YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
            DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 502 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 561

Query: 241 KLEFCRSTVGMTNSSSK 256
           KL  C S  GM +S+ +
Sbjct: 562 KLGVCCSIAGMISSNGR 578

[Close]

BLAST of CSPI05G12450 vs. NCBI nr
Match: CAN75440.1 (hypothetical protein VITISV_007304 [Vitis vinifera])

HSP 1 Score: 389.8 bits (1000), Expect = 1.8e-104
Identity = 192/257 (74.71%), Postives = 219/257 (85.21%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS  K VSSPL SHFKL S+ SPSTDKEKEDM +V YASA+GSLMY M+CTRPDIA+ +G
Sbjct: 1046 MSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRPDIAYAVG 1105

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+RKSTS
Sbjct: 1106 VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRKSTS 1165

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA  EA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1166 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFKQQRYVVY 1225

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1226 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1285

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+ +
Sbjct: 1286 KLGVCCSIAGMISSNGR 1302

[Close]

BLAST of CSPI05G12450 vs. NCBI nr
Match: RVW27439.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 389.8 bits (1000), Expect = 1.8e-104
Identity = 192/257 (74.71%), Postives = 219/257 (85.21%), Query Frame = 0


Query: 1    MSNAKSVSSPLPSHFKLISKQSPSTDKEKEDMSKVLYASAVGSLMYVMICTRPDIAHVIG 60
            MS  K VSSPL SHFKL S+ SPSTDKEKEDM +V YASA+GSLMY M+CTRPDIA+ +G
Sbjct: 928  MSKFKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAIGSLMYAMVCTRPDIAYAVG 987

Query: 61   -VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGDEKSVLVGYTDSDMTVDLDSRKSTS 120
             VS F+SN G+ HWEAVKWIMRYLR TS LKLTFG  K +LVGYTDSDM  D+D+RKSTS
Sbjct: 988  VVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYTDSDMAGDVDNRKSTS 1047

Query: 121  GYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAEAYKEMLWMKCFVQELGFKQQRYVIY 180
            GYLMTF+GGAVSWQSRLQKCVALSTT+AEYIA  EA KE+LWMKCF+QELGFKQQRYV+Y
Sbjct: 1048 GYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAVEACKELLWMKCFMQELGFKQQRYVVY 1107

Query: 181  YDNQS-VHLGKNDSFHSRTKHIDVRYHWLRDALNDKLFELEKIHTDHNGSDMLTKNLPRS 240
             DNQS +HL KN ++H+R+KHIDVRYHW+RDALND LFE+EKIHTD+NGSDMLTK LPR 
Sbjct: 1108 CDNQSAIHLSKNSTYHARSKHIDVRYHWMRDALNDNLFEIEKIHTDNNGSDMLTKTLPRE 1167

Query: 241  KLEFCRSTVGMTNSSSK 256
            KL  C S  GM +S+ +
Sbjct: 1168 KLGVCCSIAGMISSNGR 1184

[Close]

BLAST of CSPI05G12450 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-18
Identity = 58/177 (32.77%), Postives = 98/177 (55.37%), Query Frame = 0


Query: 37  YASAVGSLMYVMICTRPDIAHVIG-VSHFMSNLGKQHWEAVKWIMRYLRCTSSLKLTFGD 96
           Y   +G LMY+ I TR DI+  +  +S F       H +AV  I+ Y++ T    L +  
Sbjct: 378 YRRLIGRLMYLQI-TRLDISFAVNKLSQFSEAPRLAHQQAVMKILHYIKGTVGQGLFYSS 437

Query: 97  EKSV-LVGYTDSDMTVDLDSRKSTSGYLMTFAGGAVSWQSRLQKCVALSTTKAEYIAVAE 156
           +  + L  ++D+      D+R+ST+GY M      +SW+S+ Q+ V+ S+ +AEY A++ 
Sbjct: 438 QAEMQLQVFSDASFQSCKDTRRSTNGYCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSF 497

Query: 157 AYKEMLWMKCFVQELGFKQQR-YVIYYDN-QSVHLGKNDSFHSRTKHIDVRYHWLRD 210
           A  EM+W+  F +EL     +  +++ DN  ++H+  N  FH RTKHI+   H +R+
Sbjct: 498 ATDEMMWLAQFFRELQLPLSKPTLLFCDNTAAIHIATNAVFHERTKHIESDCHSVRE 553

The following BLAST results are available for this feature:

BLAST of CSPI05G12450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	1.4e-83	60.16	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	3.2e-35	34.77	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
P0CV72	1.4e-27	48.12	Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P...	[more]
Q94HW2	3.5e-26	35.29	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	4.3e-24	33.07	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]

BLAST of CSPI05G12450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438CN91	3.5e-106	75.88	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A0A438HN89	3.0e-105	75.49	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A0A438CLF9	3.0e-105	75.10	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A5C9D7	8.7e-105	74.71	Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITI...	[more]
A0A438GP95	8.7e-105	74.71	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]

BLAST of CSPI05G12450 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW24680.1	7.3e-106	75.88	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
RVW85908.1	6.2e-105	75.49	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
RVW24009.1	6.2e-105	75.10	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
CAN75440.1	1.8e-104	74.71	hypothetical protein VITISV_007304 [Vitis vinifera]	[more]
RVW27439.1	1.8e-104	74.71	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]

BLAST of CSPI05G12450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	3.6e-18	32.77	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 81..192
None	No IPR available	PANTHER	PTHR11439:SF301	SUBFAMILY NOT NAMED	coord: 81..192
None	No IPR available	CDD	cd09272	RNase_HI_RT_Ty1	coord: 101..237 e-value: 1.46551E-67 score: 203.469

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G12450.1	CSPI05G12450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding