CSPI05G12420 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G12420
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSEC12-like protein 2
LocationChr5: 11611945 .. 11615628 (-)
RNA-Seq ExpressionCSPI05G12420
SyntenyCSPI05G12420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTACGAAATAAATATTGAGAGAGAGAGGCGAAGGAGTAGACTAGATTCTCGCTTAATTTGCATTGCACCAAAATCAGACACAAAACCACAAGGGAGGCAGAGAGAGACACACAAGAAAGCTTCAAGAGAAAACGAATGGAAACTGTCGAACCTTCCAATTCCCGCAAGTATGGCGTTCCTTTCTATGCTGCCGCTTGGCTTCCTTCAGCTTCTCTCCAATCCAAACACCAACCTTCACAATCTCAAGATCCTCCACATCAATCCCCTGATCCTGACATTTCCTCCGCCGACAACAAATCAGTTCCATCTCATCCCGCCAGTTACTACGTAACTGCCGCTGGCGGTGGCGGAGAAGGTCGGAGTGGGATTTCCAATGCCCTCATACTCGCTCATTTCGATTTTGAATCCCGCTCGCTCTCTGATCACCCTGTAAACTCTCTCTCCCTCTCTGTTCTTCCCTGATTATTTCTATGATTGTCGTTGTTTGTCATTTTGTCTATGTTATTGAGTGAAGGAGAAATGGGGGCGGGTTATAATTTTGTCTTTGGATTGTTGATTCTCTTGCTTACCCGAAGGTTTCTTTAATTTTATTATCCTGTTTGTTTTGGTGATTTCTTTTTCACATGTGTATCGAAATTTGAATTGGGTGAAAATCTGAAAATCTAGGGTGTTGCCTACTTACATTGATTTTGCCATCTCAAATCTCCCGGCAGCATAATTGTCCCACACTGTCTTGCGGTTCTGGTGGTTGACCCTGTGATTTTACACTACATTTTGATGTTCTTTATTTATTATTTTTTTTAAAGAGGAAATGTGAAATTTATGTTAGCAAAGTGATGAACTGTTACAAATTATGAAGTTAAGATATCTTCGTAGTGTAGCCCCGAAGACAACAAAGGTTCTCTCTGATTTCAAATTGGCTTCGGTACATCAAGAAGAAGCAATAGAATTAATAAGGTCTTTGTTACATTTTATGATTTAATAAAAGCCTATACATCCTTGGTTCTTTTGAAAACTCTCCTATTCCTCCCTAACATACACAACTATGAAATAGCCAAGCAAGCATTAAAAGTATCTTGATATCTCTAAAAGAATGATCAAGGATCATTTGGGAGATGTATGTTCAATGCTTTATACGGATCGCTATCTGACTATTTAAATTGCTCAAGAGATCAGACGATGTTACTTGAACATGATTTGTTCTAGAGGTTGTACAATTGTGTTCTTGAGTTCTATAGTTCATTCCAACATCATTTCTTTGCTAATATATAATTTCTGTCTTGGTGAGTAAACACCTCCCATTCTATTTTCCCACCATGGTACTTTATTTTGTTGTTGTTTTGTCTATGGTTTGGTTCTGCTTACTTTCTATGCTTATAGTGTCTTTGTAATTTGAGCATTAGCATAGAAAATAAATATAATCAAACTGTTTGCTTATTTCCTTCTATTCTTCCACCAGCTGATACCTTCTTGCAAATGAATGAAGTTAACAGTACATTTGGCTATTACCATCAAATCCATTATCCTTGGCTAAAACGATTGAGCTTTTGGATTAATGCCATCAAGGCTATCATTTCCCAGGATAGGTTTGAAAAAAATCAGAGATCTTGTTAAGATGAGCATTTAGCTTGTGTCAAGATTAAACTTCTCATTGGTGTTCTCTTTCTCCTTTATTTTCAAATGACTCTTTTAATGATATTTGTTTAAAATTTTGTTAGTTATTATGAGGAGCTCTCTTTCCCCCATGGCCTTAGGTTATTTCTCTCTTTTTTATTTTTATTTATTTATTTATTTATAATTTGTCAGTGTTCTTATACTAAAAGTGATCTTTATATGAATTGCAATAGTGGGGGGCTTTCATATCCCTCATTAATTTGTTTGTTATTTTCACTCTCAATGGTAGCTCATATGCATGGACATTTTTAAGTATATTTTTTGCTATTCCGTGATATCTTTTGTTATCATTTTGCATTTTTCCCCCTCATTTCATTTTATTCCTTGTGTGCGCTAATTAGTCCACTTATCCTTCCAGGTTGCAAAATATGGAACTGGTTCTGATTTGCCGTACAGAATGGCTATTCATCCAGCTGGAGATGGAATTATTTGTTCTCTACCAAAAAGTTGCAGGTATTACCATTTCCTTTGTTAACTCCACAGCAAATCAATATGAACTGTTGATGAGGATCATAGTTCTTTCTTGGATGAACCTTCAAGTCTCAATATGCTAGTAAATGTTTTAGGTAGGCAAATATGGTTATACATATAAGAAATGTGATTTATATTCATGAAAAGTGAAAAAAAAGAAAGAAAAAAGAAATGAAAAAAAAGTAGAAAAACAGCCTAAGGGCAGGGGGGTGTTAAACCCCCTCCCACAAAACTGTTTGATATGTTATGGCATAGAATAAGAAGTTCCAGTAATTTGATGTGTTAGATCCATGTTTCTCACATTAAGGTCCTCCAACAGGCGCCATTTTGTGAAGGATGAAATATGACTGGTTAACTTGTCAATTGAGAAATAAGATTGGAGTATAATACTTTCAATCTTGAAATAAATTTGCACGTTCCTTGGTCTATTTTAATTCTATCAATGAAGTATTGACTTTTTTATTTTTTTATTATTTTATTTAGAAAAGAAAAGATTATTTAACTGCAATGTATAGTCATATCGTGTGGTTAATTTGATTGAGCCATCTATTATACCACTGTGTAACTTGAGAAGTGTTGATTTTTAATTAATATTCTGCTCACTTTATTTAGCCTGTTTAAAGTGGATACTGAGAAGGATGCTGGAGATGAGACACTTGGACTAAAACTCTCTCAGGAGGTCCTCAGCCCCTTGGAAGATGTTGGACAGCAGTTATCATTGGCATTTAACAACGAGGGCTCTTTGCTTGCCACTGGTGGTGAGGTAGGAAATTTGAAGATTTGATTGCTTACTATTACTATTATTTCTTTTGGATAGGAAAACAATTTTTTATCCACCCATAATTTAAACTTTGAGAAGGGTGAAGGCAAGGTACTCTCCAGGCAAGCAAAAGGAGCTTACAAATTCAATCTTAATGTTTATTGCCAATGCATTTCTCAACTTACTAGCCGTTTCTAACCCTAGACCTTTTCTATAATGTCATTTTTCTCTTGAAATAAATACCAATTTCCACTTATTGGACTTTTGACAAATTTCCAAGCCCATAAGTGATAGTGTTCAAATATTAACATAATATGACTTCAAGTTTTTTTTTGTTTTTGGGTTGAATGGTGATTTAACACATTGGTTAAATACTATAGGATGCTCGATAATTATTGAGAAGTAGCTTGCATTATTGTTTTCTGATTTGAATACAGTACAGGATGGCAATTTAAGGGTCTTAAAGTGGCCTAGCTTGGACATTGTTCTCAATGAGCCCAGTTCCCATTCTTCGGTCAAGGACTTGGATTTCAGGTCTGCAATGTTATAAATTATTTTTCTTGATAGCTCTTGCATATGTTAGACAATATGATATGTGTATGTTGTTTGTTTATCTAAAGTCTCTTGTGTAATGAAGTTATTGTGATAGCTGTCATTTTATTTTTGCAGTCCTGATGGCAAATATCTTGTCTCCTTAGGTGGCCCTTGTAGAGTGTGGGATATAACTTCATCGACTTTAGTAACCTCTCTTCCGAAAGAAAATGTGAGCAAATAG

mRNA sequence

AAAATTACGAAATAAATATTGAGAGAGAGAGGCGAAGGAGTAGACTAGATTCTCGCTTAATTTGCATTGCACCAAAATCAGACACAAAACCACAAGGGAGGCAGAGAGAGACACACAAGAAAGCTTCAAGAGAAAACGAATGGAAACTGTCGAACCTTCCAATTCCCGCAAGTATGGCGTTCCTTTCTATGCTGCCGCTTGGCTTCCTTCAGCTTCTCTCCAATCCAAACACCAACCTTCACAATCTCAAGATCCTCCACATCAATCCCCTGATCCTGACATTTCCTCCGCCGACAACAAATCAGTTCCATCTCATCCCGCCAGTTACTACGTAACTGCCGCTGGCGGTGGCGGAGAAGGTCGGAGTGGGATTTCCAATGCCCTCATACTCGCTCATTTCGATTTTGAATCCCGCTCGCTCTCTGATCACCCTGTTGCAAAATATGGAACTGGTTCTGATTTGCCGTACAGAATGGCTATTCATCCAGCTGGAGATGGAATTATTTGTTCTCTACCAAAAAGTTGCAGCCTGTTTAAAGTGGATACTGAGAAGGATGCTGGAGATGAGACACTTGGACTAAAACTCTCTCAGGAGGTCCTCAGCCCCTTGGAAGATGTTGGACAGCAGTTATCATTGGCATTTAACAACGAGGGCTCTTTGCTTGCCACTGGTGGTGAGGATGGCAATTTAAGGGTCTTAAAGTGGCCTAGCTTGGACATTGTTCTCAATGAGCCCAGTTCCCATTCTTCGGTCAAGGACTTGGATTTCAGTCCTGATGGCAAATATCTTGTCTCCTTAGGTGGCCCTTGTAGAGTGTGGGATATAACTTCATCGACTTTAGTAACCTCTCTTCCGAAAGAAAATGTGAGCAAATAG

Coding sequence (CDS)

ATGGAAACTGTCGAACCTTCCAATTCCCGCAAGTATGGCGTTCCTTTCTATGCTGCCGCTTGGCTTCCTTCAGCTTCTCTCCAATCCAAACACCAACCTTCACAATCTCAAGATCCTCCACATCAATCCCCTGATCCTGACATTTCCTCCGCCGACAACAAATCAGTTCCATCTCATCCCGCCAGTTACTACGTAACTGCCGCTGGCGGTGGCGGAGAAGGTCGGAGTGGGATTTCCAATGCCCTCATACTCGCTCATTTCGATTTTGAATCCCGCTCGCTCTCTGATCACCCTGTTGCAAAATATGGAACTGGTTCTGATTTGCCGTACAGAATGGCTATTCATCCAGCTGGAGATGGAATTATTTGTTCTCTACCAAAAAGTTGCAGCCTGTTTAAAGTGGATACTGAGAAGGATGCTGGAGATGAGACACTTGGACTAAAACTCTCTCAGGAGGTCCTCAGCCCCTTGGAAGATGTTGGACAGCAGTTATCATTGGCATTTAACAACGAGGGCTCTTTGCTTGCCACTGGTGGTGAGGATGGCAATTTAAGGGTCTTAAAGTGGCCTAGCTTGGACATTGTTCTCAATGAGCCCAGTTCCCATTCTTCGGTCAAGGACTTGGATTTCAGTCCTGATGGCAAATATCTTGTCTCCTTAGGTGGCCCTTGTAGAGTGTGGGATATAACTTCATCGACTTTAGTAACCTCTCTTCCGAAAGAAAATGTGAGCAAATAG

Protein sequence

METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHPASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDGIICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPKENVSK*
Homology
BLAST of CSPI05G12420 vs. ExPASy Swiss-Prot
Match: Q39221 (SEC12-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=STL2P PE=1 SV=4)

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-59
Identity = 123/241 (51.04%), Postives = 156/241 (64.73%), Query Frame = 0

Query: 2   ETVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHPA 61
           ET +PSN + YGVP YAA W+P   ++SK         P +S D D SS+ + S  S   
Sbjct: 7   ETNQPSNMQTYGVPIYAADWIPEVDVRSKIIMD-----PEKSEDDDESSSSSSSSRS--- 66

Query: 62  SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDGI 121
              +  +GGGGEGRSGISN +++   D  + SLS+ P+ +   GSDLPYRMA+HP   G+
Sbjct: 67  --CIVLSGGGGEGRSGISNVILICRVDLNTNSLSEQPLGRLVVGSDLPYRMAVHPREGGL 126

Query: 122 ICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGED 181
           IC+LP SC LF  +      D   G   S+EV+  L DVGQQL+LAFN EGS+LA G ED
Sbjct: 127 ICALPNSCKLFHWEDIMSREDNQAG--ESEEVIKELRDVGQQLALAFNPEGSVLAAGAED 186

Query: 182 GNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGP-CRVWDITSSTLVTSLPK 241
           G LRV KWPS++ +LNE  +HSSVK L FS  G++LVSLGGP CRVWD+ +S  V SL K
Sbjct: 187 GTLRVFKWPSMNTLLNESQAHSSVKCLTFSESGQFLVSLGGPVCRVWDVNASAAVASLSK 235

BLAST of CSPI05G12420 vs. ExPASy Swiss-Prot
Match: Q8GYE0 (SEC12-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PHF1 PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 1.0e-22
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 76  SGISNALILAHFDFE--SRSLSDHPVAKY--GTGSDLPYRMAIHPAGDGIICSLPK-SCS 135
           S +S+  +L  F F+  + SLS  P+A +        P  +++HP GD  +CS  K  C 
Sbjct: 39  SSVSSPALLNIFSFDPITASLSSSPLATHTLKDSDGDPVAVSVHPGGDYFVCSTSKGGCK 98

Query: 136 LFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGNLRVLKWP 195
           LF++      G  T G+ +  + L PL++ G Q  +AF+ +GS LA GG DG LR+++WP
Sbjct: 99  LFEL-----VGGAT-GITILAKELLPLQNAGLQKCMAFSFDGSKLAVGGVDGCLRIMEWP 158

Query: 196 SLDIVLNEPSSHSSVKDLDFSPDGKYL--VSLGGPCRVW 228
           +L ++L+EP +H S++D+DFS D ++L   S  G  R+W
Sbjct: 159 NLSVILDEPKAHKSIRDMDFSLDSEFLATTSTDGSARIW 191

BLAST of CSPI05G12420 vs. ExPASy Swiss-Prot
Match: Q9HCU5 (Prolactin regulatory element-binding protein OS=Homo sapiens OX=9606 GN=PREB PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 3.1e-11
Identity = 62/212 (29.25%), Postives = 92/212 (43.40%), Query Frame = 0

Query: 60  PASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGD 119
           P++  + AAGGGG  ++GI N +     +  +  LS   +  + T +     +A+  AGD
Sbjct: 23  PSTGLLIAAGGGGAAKTGIKNGVHFLQLELINGRLSASLLHSHDTETRATMNLAL--AGD 82

Query: 120 GIICSLPKSCSLFK---------------------------VDTEKDAGDETL--GLKLS 179
            +       C L +                              EK  G ET   GL+L 
Sbjct: 83  ILAAGQDAHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELR 142

Query: 180 QEVLSPLE-DVGQ---QLSLAFNNEGSLLATGGEDGNLRVLKWPSLDIVLNEPSSHSSVK 239
            E L  ++ D      Q  + FN++ +LLATGG DG +RV K PSL+ VL   +    ++
Sbjct: 143 VENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIE 202

BLAST of CSPI05G12420 vs. ExPASy Swiss-Prot
Match: Q9WUQ2 (Prolactin regulatory element-binding protein OS=Mus musculus OX=10090 GN=Preb PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.9e-11
Identity = 58/217 (26.73%), Postives = 90/217 (41.47%), Query Frame = 0

Query: 60  PASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGD 119
           P +  + AAGGGG  ++GI N +     +  +  LS   +  + T +     +A+  AGD
Sbjct: 23  PKTGLLIAAGGGGAAKTGIKNGVHFLQLELINGCLSASLLHSHDTETRATMNLAL--AGD 82

Query: 120 GIICSLPKSCSLFKVDTEKDAGDET---------------------------------LG 179
            +       C L +    +  G +                                  L 
Sbjct: 83  ILAAGQDAQCQLLRFQVHQQKGSKAEKSGSKEQGPRQRKGAPPAEKKSGAQVHPEGVELK 142

Query: 180 LKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGNLRVLKWPSLDIVLNEPSSHSSVK 239
           +K  + V +   +   Q  + FN++ +LLATGG DG++RV K PSL+ VL   +    + 
Sbjct: 143 VKNLEAVQTDFSNEPLQKVVCFNHDNTLLATGGTDGHVRVWKVPSLEKVLEFKAHEGEIG 202

BLAST of CSPI05G12420 vs. ExPASy Swiss-Prot
Match: Q9WTV0 (Prolactin regulatory element-binding protein OS=Rattus norvegicus OX=10116 GN=Preb PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-10
Identity = 62/217 (28.57%), Postives = 91/217 (41.94%), Query Frame = 0

Query: 60  PASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGD 119
           P +  + AAGGGG  ++GI N +     +  S  LS   +  + T +     +A+  AGD
Sbjct: 23  PKTGLLIAAGGGGAAKTGIKNGVHFLQLEQISGCLSASLLHSHDTETRATMNLAL--AGD 82

Query: 120 GIICSLPKSCSLFK---------------------------VDTEKDAGDET------LG 179
            +       C L +                              EK +G E       L 
Sbjct: 83  ILAAGQDAQCQLLRFQIHQQKGSKAEKSGSKEQGPRQRKGAAPAEKKSGAEVHPEGVELK 142

Query: 180 LKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGNLRVLKWPSLDIVLNEPSSHSSVK 239
           +K  + V +       Q  + FN++ +LLATGG DG++RV K PSL+ VL   +    + 
Sbjct: 143 VKNLEAVQTDFSTEPLQKVVCFNHDNTLLATGGSDGHVRVWKVPSLEKVLEFKAHEGEIG 202

BLAST of CSPI05G12420 vs. ExPASy TrEMBL
Match: A0A0A0KMF5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286090 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 4.6e-135
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           M TVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKS+PSHP
Sbjct: 1   MGTVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSLPSHP 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE
Sbjct: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 ENVSK 246
           ENVSK
Sbjct: 241 ENVSK 245

BLAST of CSPI05G12420 vs. ExPASy TrEMBL
Match: A0A5A7VE72 (SEC12-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G00330 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 224/242 (92.56%), Postives = 231/242 (95.45%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           MET EPSNSRKYGVPFYAAAW PS SL+S+HQPSQS+DPPHQS D DIS+ADNKSVPSH 
Sbjct: 1   METPEPSNSRKYGVPFYAAAWFPSTSLKSEHQPSQSEDPPHQSADADISAADNKSVPSHL 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDF+SRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFDSRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPK+C LFKVDTEKDAGD  LGLKLSQEVLSPLEDVGQQLSLAFN EGSLLATGGE
Sbjct: 121 IICSLPKNCCLFKVDTEKDAGDGKLGLKLSQEVLSPLEDVGQQLSLAFNKEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHS+VKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSTVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 EN 243
           EN
Sbjct: 241 EN 242

BLAST of CSPI05G12420 vs. ExPASy TrEMBL
Match: A0A1S3C056 (SEC12-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103495101 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 224/242 (92.56%), Postives = 231/242 (95.45%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           MET EPSNSRKYGVPFYAAAW PS SL+S+HQPSQS+DPPHQS D DIS+ADNKSVPSH 
Sbjct: 1   METPEPSNSRKYGVPFYAAAWFPSTSLKSEHQPSQSEDPPHQSADADISAADNKSVPSHL 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDF+SRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFDSRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPK+C LFKVDTEKDAGD  LGLKLSQEVLSPLEDVGQQLSLAFN EGSLLATGGE
Sbjct: 121 IICSLPKNCCLFKVDTEKDAGDGKLGLKLSQEVLSPLEDVGQQLSLAFNKEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHS+VKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSTVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 EN 243
           EN
Sbjct: 241 EN 242

BLAST of CSPI05G12420 vs. ExPASy TrEMBL
Match: A0A6J1FTE5 (SEC12-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111447960 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.7e-105
Identity = 199/246 (80.89%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQ-DPPHQSPDPDISSADNKSVPSH 60
           MET +PS SRKYGVPFYAAAWLP  SL S  +P QS+ D P QS D  I SA++KS PSH
Sbjct: 1   METPDPSYSRKYGVPFYAAAWLPFTSLNSHQEPPQSENDHPDQSNDAHI-SANHKSPPSH 60

Query: 61  PA---SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHP 120
           P    +YY+ AAGGGGEGRSGISNAL+LA+FDFESRSLSD PVAKYGTGSDLPYRMA+HP
Sbjct: 61  PTAALNYYIAAAGGGGEGRSGISNALLLAYFDFESRSLSDQPVAKYGTGSDLPYRMAVHP 120

Query: 121 AGDGIICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLA 180
           AGDGIICSLPKSCSLFK+DT+KDAGD  LGLKLSQEVLS LEDVG QL+LAFNNEGSLLA
Sbjct: 121 AGDGIICSLPKSCSLFKLDTKKDAGDNKLGLKLSQEVLSQLEDVGLQLALAFNNEGSLLA 180

Query: 181 TGGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVT 240
           TGGEDGNLRV KWPSLDI+LNEPS+HSSVKDLDFS DGKYLVSLGGPCRVWD+TSS++VT
Sbjct: 181 TGGEDGNLRVFKWPSLDIILNEPSAHSSVKDLDFSYDGKYLVSLGGPCRVWDVTSSSVVT 240

Query: 241 SLPKEN 243
           SL KEN
Sbjct: 241 SLTKEN 245

BLAST of CSPI05G12420 vs. ExPASy TrEMBL
Match: A0A6J1J4E0 (SEC12-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111483300 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.2e-104
Identity = 197/246 (80.08%), Postives = 215/246 (87.40%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQ-DPPHQSPDPDISSADNKSVPSH 60
           MET +PSNSRKYGVPFYAAAWLP  SL S  +P QS+ D P QS D  I SA+ KS PSH
Sbjct: 1   METPDPSNSRKYGVPFYAAAWLPFTSLNSHQEPPQSENDHPDQSNDAHI-SANPKSPPSH 60

Query: 61  PA---SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHP 120
           P    +YY+ AAGGGGEGRSGISNAL+LA+FDF+SRSLS  PVAKYGTGSDLPYRMA+HP
Sbjct: 61  PTAALNYYIAAAGGGGEGRSGISNALLLAYFDFQSRSLSHQPVAKYGTGSDLPYRMAVHP 120

Query: 121 AGDGIICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLA 180
           AGDGIICSLPKSCSLFK+DT+KDAGD  LGLKLSQEVLS L DVG QL+LAFNNEGSLLA
Sbjct: 121 AGDGIICSLPKSCSLFKLDTKKDAGDNKLGLKLSQEVLSQLADVGLQLALAFNNEGSLLA 180

Query: 181 TGGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVT 240
           TGGEDGNLRV KWPSLDI+LNEPS+HSSVKDLDFS DGKYLVSLGGPCRVWD+TSS++VT
Sbjct: 181 TGGEDGNLRVFKWPSLDIILNEPSAHSSVKDLDFSYDGKYLVSLGGPCRVWDVTSSSVVT 240

Query: 241 SLPKEN 243
           SL KEN
Sbjct: 241 SLTKEN 245

BLAST of CSPI05G12420 vs. NCBI nr
Match: XP_004140431.1 (SEC12-like protein 2 [Cucumis sativus] >KGN50716.2 hypothetical protein Csa_004731 [Cucumis sativus])

HSP 1 Score: 485.3 bits (1248), Expect = 3.0e-133
Identity = 239/242 (98.76%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           M TVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKS+PSHP
Sbjct: 1   MGTVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSLPSHP 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE
Sbjct: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 EN 243
           EN
Sbjct: 241 EN 242

BLAST of CSPI05G12420 vs. NCBI nr
Match: XP_008454782.1 (PREDICTED: SEC12-like protein 2 [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 2.6e-124
Identity = 224/242 (92.56%), Postives = 231/242 (95.45%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           MET EPSNSRKYGVPFYAAAW PS SL+S+HQPSQS+DPPHQS D DIS+ADNKSVPSH 
Sbjct: 1   METPEPSNSRKYGVPFYAAAWFPSTSLKSEHQPSQSEDPPHQSADADISAADNKSVPSHL 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDF+SRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFDSRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPK+C LFKVDTEKDAGD  LGLKLSQEVLSPLEDVGQQLSLAFN EGSLLATGGE
Sbjct: 121 IICSLPKNCCLFKVDTEKDAGDGKLGLKLSQEVLSPLEDVGQQLSLAFNKEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHS+VKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSTVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 EN 243
           EN
Sbjct: 241 EN 242

BLAST of CSPI05G12420 vs. NCBI nr
Match: KAA0065998.1 (SEC12-like protein 2 [Cucumis melo var. makuwa] >TYK28946.1 SEC12-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 455.7 bits (1171), Expect = 2.6e-124
Identity = 224/242 (92.56%), Postives = 231/242 (95.45%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           MET EPSNSRKYGVPFYAAAW PS SL+S+HQPSQS+DPPHQS D DIS+ADNKSVPSH 
Sbjct: 1   METPEPSNSRKYGVPFYAAAWFPSTSLKSEHQPSQSEDPPHQSADADISAADNKSVPSHL 60

Query: 61  ASYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120
           ASYYV AAGGGGEGRSGISNALILAHFDF+SRSLSDHPVAKYGTGSDLPYRMAIHPAGDG
Sbjct: 61  ASYYVAAAGGGGEGRSGISNALILAHFDFDSRSLSDHPVAKYGTGSDLPYRMAIHPAGDG 120

Query: 121 IICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGE 180
           IICSLPK+C LFKVDTEKDAGD  LGLKLSQEVLSPLEDVGQQLSLAFN EGSLLATGGE
Sbjct: 121 IICSLPKNCCLFKVDTEKDAGDGKLGLKLSQEVLSPLEDVGQQLSLAFNKEGSLLATGGE 180

Query: 181 DGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240
           DGNLRVLKWPSLDIVLNEPSSHS+VKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK
Sbjct: 181 DGNLRVLKWPSLDIVLNEPSSHSTVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTSLPK 240

Query: 241 EN 243
           EN
Sbjct: 241 EN 242

BLAST of CSPI05G12420 vs. NCBI nr
Match: XP_038892140.1 (SEC12-like protein 2 [Benincasa hispida])

HSP 1 Score: 408.7 bits (1049), Expect = 3.6e-110
Identity = 202/245 (82.45%), Postives = 220/245 (89.80%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHP 60
           ME  +PSNSRKYG+PFYA AWLPS SL+S+ +P +S++   QS D DIS+ DNKS+PSHP
Sbjct: 1   MEAPQPSNSRKYGIPFYAVAWLPSTSLKSQQEPPESENHSDQSADADISADDNKSLPSHP 60

Query: 61  A---SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPA 120
           A   +YYV A GGGGEGRSGISNALILAHFDFESRSLSD PVAKYGTGSDLPYRMA+HPA
Sbjct: 61  ATADNYYVAATGGGGEGRSGISNALILAHFDFESRSLSDQPVAKYGTGSDLPYRMAVHPA 120

Query: 121 GDGIICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLAT 180
           GDGIICSLPKSCSLFKVDT+KDA DE LGLKLSQEVLS LEDVGQQL+L FNN GSLLAT
Sbjct: 121 GDGIICSLPKSCSLFKVDTKKDARDEKLGLKLSQEVLSQLEDVGQQLALTFNNGGSLLAT 180

Query: 181 GGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVTS 240
           GGEDGNLRVLKWPSLDIVLNEPS+HS+VKDLDFS D KYLVSLGGPCRVWD+T+STLVTS
Sbjct: 181 GGEDGNLRVLKWPSLDIVLNEPSAHSTVKDLDFSLDDKYLVSLGGPCRVWDVTTSTLVTS 240

Query: 241 LPKEN 243
           LPKEN
Sbjct: 241 LPKEN 245

BLAST of CSPI05G12420 vs. NCBI nr
Match: KAG6599876.1 (SEC12-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 394.8 bits (1013), Expect = 5.4e-106
Identity = 199/246 (80.89%), Postives = 218/246 (88.62%), Query Frame = 0

Query: 1   METVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQ-DPPHQSPDPDISSADNKSVPSH 60
           MET +PSNSRKYGVPFYAAAWLP  SL S  +P QS+ D P QS D  I SA++KS PSH
Sbjct: 1   METPDPSNSRKYGVPFYAAAWLPFTSLNSHQEPPQSENDHPDQSNDAHI-SANHKSPPSH 60

Query: 61  PA---SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHP 120
           P    +YY+ AAGGGGEGRSGISNAL+LA+FDFESRSLSD P+AKYGTGSDLPYRMA+HP
Sbjct: 61  PTAALNYYIAAAGGGGEGRSGISNALLLAYFDFESRSLSDQPIAKYGTGSDLPYRMAVHP 120

Query: 121 AGDGIICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLA 180
           AGDGIICSLPKSCSLFK+DT+KDAGD  LGLKLSQEVLS LEDVG QL+LAFNNEGSLLA
Sbjct: 121 AGDGIICSLPKSCSLFKLDTKKDAGDNKLGLKLSQEVLSQLEDVGLQLALAFNNEGSLLA 180

Query: 181 TGGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGPCRVWDITSSTLVT 240
           TGGEDGNLRV KWPSLDI+LNEPS+HSSVKDLDFS DGKYLVSLGGPCRVWD+TSS++VT
Sbjct: 181 TGGEDGNLRVFKWPSLDIILNEPSAHSSVKDLDFSYDGKYLVSLGGPCRVWDVTSSSVVT 240

Query: 241 SLPKEN 243
           SL KEN
Sbjct: 241 SLTKEN 245

BLAST of CSPI05G12420 vs. TAIR 10
Match: AT2G01470.1 (SEC12P-like 2 protein )

HSP 1 Score: 231.1 bits (588), Expect = 9.6e-61
Identity = 123/241 (51.04%), Postives = 156/241 (64.73%), Query Frame = 0

Query: 2   ETVEPSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHPA 61
           ET +PSN + YGVP YAA W+P   ++SK         P +S D D SS+ + S  S   
Sbjct: 7   ETNQPSNMQTYGVPIYAADWIPEVDVRSKIIMD-----PEKSEDDDESSSSSSSSRS--- 66

Query: 62  SYYVTAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDGI 121
              +  +GGGGEGRSGISN +++   D  + SLS+ P+ +   GSDLPYRMA+HP   G+
Sbjct: 67  --CIVLSGGGGEGRSGISNVILICRVDLNTNSLSEQPLGRLVVGSDLPYRMAVHPREGGL 126

Query: 122 ICSLPKSCSLFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGED 181
           IC+LP SC LF  +      D   G   S+EV+  L DVGQQL+LAFN EGS+LA G ED
Sbjct: 127 ICALPNSCKLFHWEDIMSREDNQAG--ESEEVIKELRDVGQQLALAFNPEGSVLAAGAED 186

Query: 182 GNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGP-CRVWDITSSTLVTSLPK 241
           G LRV KWPS++ +LNE  +HSSVK L FS  G++LVSLGGP CRVWD+ +S  V SL K
Sbjct: 187 GTLRVFKWPSMNTLLNESQAHSSVKCLTFSESGQFLVSLGGPVCRVWDVNASAAVASLSK 235

BLAST of CSPI05G12420 vs. TAIR 10
Match: AT5G50550.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 114/239 (47.70%), Postives = 151/239 (63.18%), Query Frame = 0

Query: 6   PSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHPASYYV 65
           PSN + YGVP YA  W+   +++SK    Q  D      D D SS          +S Y+
Sbjct: 8   PSNLQTYGVPIYAVDWILEEAVRSKITKDQDDD------DDDGSS----------SSSYI 67

Query: 66  TAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDGIICSL 125
             +GGGGEGRSGI N +++   D  + SLS+ P+ +   G+DLPYRMAIHP   G+IC+L
Sbjct: 68  VLSGGGGEGRSGIPNVILICRVDLHTNSLSEQPIGRRVIGTDLPYRMAIHPRQGGLICAL 127

Query: 126 PKSCSLFKVDT--EKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGN 185
           P SC LF  +   E D  +E      S++V+  L+DVGQQLSL+FN +G++LATG EDG 
Sbjct: 128 PNSCRLFDWENIIEDDNEEE------SEKVVKELKDVGQQLSLSFNQDGTVLATGAEDGT 187

Query: 186 LRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGP-CRVWDITSSTLVTSLPKE 242
           LRV +WPS+  +LNE  +H+SVK L FS  GK+LVSLG P CRVWD+ +S  + SL KE
Sbjct: 188 LRVFEWPSMKTLLNESKTHASVKSLTFSESGKFLVSLGAPLCRVWDVNASAAIASLSKE 224

BLAST of CSPI05G12420 vs. TAIR 10
Match: AT5G50650.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 114/239 (47.70%), Postives = 151/239 (63.18%), Query Frame = 0

Query: 6   PSNSRKYGVPFYAAAWLPSASLQSKHQPSQSQDPPHQSPDPDISSADNKSVPSHPASYYV 65
           PSN + YGVP YA  W+   +++SK    Q  D      D D SS          +S Y+
Sbjct: 8   PSNLQTYGVPIYAVDWILEEAVRSKITKDQDDD------DDDGSS----------SSSYI 67

Query: 66  TAAGGGGEGRSGISNALILAHFDFESRSLSDHPVAKYGTGSDLPYRMAIHPAGDGIICSL 125
             +GGGGEGRSGI N +++   D  + SLS+ P+ +   G+DLPYRMAIHP   G+IC+L
Sbjct: 68  VLSGGGGEGRSGIPNVILICRVDLHTNSLSEQPIGRRVIGTDLPYRMAIHPRQGGLICAL 127

Query: 126 PKSCSLFKVDT--EKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGN 185
           P SC LF  +   E D  +E      S++V+  L+DVGQQLSL+FN +G++LATG EDG 
Sbjct: 128 PNSCRLFDWENIIEDDNEEE------SEKVVKELKDVGQQLSLSFNQDGTVLATGAEDGT 187

Query: 186 LRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGP-CRVWDITSSTLVTSLPKE 242
           LRV +WPS+  +LNE  +H+SVK L FS  GK+LVSLG P CRVWD+ +S  + SL KE
Sbjct: 188 LRVFEWPSMKTLLNESKTHASVKSLTFSESGKFLVSLGAPLCRVWDVNASAAIASLSKE 224

BLAST of CSPI05G12420 vs. TAIR 10
Match: AT3G52190.1 (phosphate transporter traffic facilitator1 )

HSP 1 Score: 108.6 bits (270), Expect = 7.2e-24
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 76  SGISNALILAHFDFE--SRSLSDHPVAKY--GTGSDLPYRMAIHPAGDGIICSLPK-SCS 135
           S +S+  +L  F F+  + SLS  P+A +        P  +++HP GD  +CS  K  C 
Sbjct: 39  SSVSSPALLNIFSFDPITASLSSSPLATHTLKDSDGDPVAVSVHPGGDYFVCSTSKGGCK 98

Query: 136 LFKVDTEKDAGDETLGLKLSQEVLSPLEDVGQQLSLAFNNEGSLLATGGEDGNLRVLKWP 195
           LF++      G  T G+ +  + L PL++ G Q  +AF+ +GS LA GG DG LR+++WP
Sbjct: 99  LFEL-----VGGAT-GITILAKELLPLQNAGLQKCMAFSFDGSKLAVGGVDGCLRIMEWP 158

Query: 196 SLDIVLNEPSSHSSVKDLDFSPDGKYL--VSLGGPCRVW 228
           +L ++L+EP +H S++D+DFS D ++L   S  G  R+W
Sbjct: 159 NLSVILDEPKAHKSIRDMDFSLDSEFLATTSTDGSARIW 191

BLAST of CSPI05G12420 vs. TAIR 10
Match: AT2G37160.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.6e-05
Identity = 25/73 (34.25%), Postives = 42/73 (57.53%), Query Frame = 0

Query: 165 SLAFNNEGSLLATGGEDGNLRVLKWPSLDIVLNEPSSHSSVKDLDFSPDGKYLVSLGGP- 224
           S+AF+N+G+ LAT G DG LR+  + +  +V    S + ++    +S DGKYL++ G   
Sbjct: 308 SIAFSNDGAYLATVGRDGYLRIFDFSTQKLVCGVKSYYGALLCCAWSMDGKYLLTGGEDD 367

Query: 225 -CRVWDITSSTLV 236
             +VW +    +V
Sbjct: 368 LVQVWSMEDRKVV 380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q392211.4e-5951.04SEC12-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=STL2P PE=1 SV=4[more]
Q8GYE01.0e-2237.11SEC12-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PHF1 PE=1 SV=2[more]
Q9HCU53.1e-1129.25Prolactin regulatory element-binding protein OS=Homo sapiens OX=9606 GN=PREB PE=... [more]
Q9WUQ28.9e-1126.73Prolactin regulatory element-binding protein OS=Mus musculus OX=10090 GN=Preb PE... [more]
Q9WTV01.2e-1028.57Prolactin regulatory element-binding protein OS=Rattus norvegicus OX=10116 GN=Pr... [more]
Match NameE-valueIdentityDescription
A0A0A0KMF54.6e-13598.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286090 PE=4 SV=1[more]
A0A5A7VE721.2e-12492.56SEC12-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120... [more]
A0A1S3C0561.2e-12492.56SEC12-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103495101 PE=4 SV=1[more]
A0A6J1FTE51.7e-10580.89SEC12-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111447960 PE=4 SV=1[more]
A0A6J1J4E03.2e-10480.08SEC12-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111483300 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004140431.13.0e-13398.76SEC12-like protein 2 [Cucumis sativus] >KGN50716.2 hypothetical protein Csa_0047... [more]
XP_008454782.12.6e-12492.56PREDICTED: SEC12-like protein 2 [Cucumis melo][more]
KAA0065998.12.6e-12492.56SEC12-like protein 2 [Cucumis melo var. makuwa] >TYK28946.1 SEC12-like protein 2... [more]
XP_038892140.13.6e-11082.45SEC12-like protein 2 [Benincasa hispida][more]
KAG6599876.15.4e-10680.89SEC12-like protein 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G01470.19.6e-6151.04SEC12P-like 2 protein [more]
AT5G50550.11.2e-5547.70Transducin/WD40 repeat-like superfamily protein [more]
AT5G50650.11.2e-5547.70Transducin/WD40 repeat-like superfamily protein [more]
AT3G52190.17.2e-2437.11phosphate transporter traffic facilitator1 [more]
AT2G37160.12.6e-0534.25Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 150..188
e-value: 0.4
score: 19.5
coord: 192..228
e-value: 1.7
score: 15.5
IPR001680WD40 repeatPFAMPF00400WD40coord: 198..219
e-value: 0.038
score: 14.9
coord: 165..186
e-value: 0.057
score: 14.3
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 55..245
e-value: 4.5E-34
score: 120.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..60
NoneNo IPR availablePANTHERPTHR23284PROLACTIN REGULATORY ELEMENT BINDING PROTEINcoord: 5..242
NoneNo IPR availablePANTHERPTHR23284:SF5SEC12P-LIKE 2 PROTEIN-RELATEDcoord: 5..242
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 103..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G12420.1CSPI05G12420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016036 cellular response to phosphate starvation
biological_process GO:0006817 phosphate ion transport
biological_process GO:0009306 protein secretion
biological_process GO:0003400 regulation of COPII vesicle coating
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0005515 protein binding