CSPI05G12330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G12330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Serine proteinase inhibitor
Location	Chr5: 11504005 .. 11504837 (+)
RNA-Seq Expression	CSPI05G12330
Synteny	CSPI05G12330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCGGCATACCCTTAGGTTCTCTGAATTTTCTAAACTCATTCATAACATTTTTCCCTATAAATACTCACAACTCTTCCCTATTTGCATTATCCAAACACTAATTCTTCAACAAATACATTCAAACCCAAATTCACATTCCTCAACTTCAACCAAACGATGGCTGATATTTGTCGTCCTGGTATGTTTCCATTTATGTTTTTACATTACTACAAGTTTTGAGACACTCTATACATTTTATAAAGTGTGTTCCTGAGTTTTCTATTTCAATGTTTACAATTGGGGTTAGATTTTTTGTAATCGAATATACCCATTAGAAGCATCTTTTTGCATAGAATGTGTTCCTGAGTTTTGCTTCTTCATTATAATGATGAGATACTTCTGTTGATGATCTTTAGACTGTAGTTTTGTTTGGATTATCTCTTTGGATTTTTCATATGGTCATGATTATTTGATTATTTGATTATTTGTTTTGGTATATAATTATGATTAGAGAAAAACCAGTGGCCAGAACTTGTTGGAATAAAAGCAACAATTGCGGAGTATATCATCAAGAAAGACAATCCTAATGTAGAAAATGTTAGAGTTCTCTTGGCTGGAACTGGAACCACTAAAGATTTTAGGAGTGATCGAGTTTGGTTGTTTACTAATATAGACGGGCGAGTTGTTGAAATGCCAACGGTGGGTTAGATAAAAGACTATCAAAATACGTTGTATGCTTTTATTTGTATTCCTAAATCACTTTCTAAAACTATTGATGTAAGCAAAATAAGGGTAGAAACTATACCATATATATTAATATTTCTACCACATGTTTAATTATCAATTTCCCTTT

mRNA sequence

GGCGGCATACCCTTAGGTTCTCTGAATTTTCTAAACTCATTCATAACATTTTTCCCTATAAATACTCACAACTCTTCCCTATTTGCATTATCCAAACACTAATTCTTCAACAAATACATTCAAACCCAAATTCACATTCCTCAACTTCAACCAAACGATGGCTGATATTTGTCGTCCTGAGAAAAACCAGTGGCCAGAACTTGTTGGAATAAAAGCAACAATTGCGGAGTATATCATCAAGAAAGACAATCCTAATGTAGAAAATGTTAGAGTTCTCTTGGCTGGAACTGGAACCACTAAAGATTTTAGGAGTGATCGAGTTTGGTTGTTTACTAATATAGACGGGCGAGTTGTTGAAATGCCAACGGTGGGTTAGATAAAAGACTATCAAAATACGTTGTATGCTTTTATTTGTATTCCTAAATCACTTTCTAAAACTATTGATGTAAGCAAAATAAGGGTAGAAACTATACCATATATATTAATATTTCTACCACATGTTTAATTATCAATTTCCCTTT

Coding sequence (CDS)

ATGGCTGATATTTGTCGTCCTGAGAAAAACCAGTGGCCAGAACTTGTTGGAATAAAAGCAACAATTGCGGAGTATATCATCAAGAAAGACAATCCTAATGTAGAAAATGTTAGAGTTCTCTTGGCTGGAACTGGAACCACTAAAGATTTTAGGAGTGATCGAGTTTGGTTGTTTACTAATATAGACGGGCGAGTTGTTGAAATGCCAACGGTGGGTTAG

Protein sequence

MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEMPTVG*

Homology

[Close]

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.2e-13
Identity = 34/63 (53.97%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 9   KNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 68
           K  WPEL+G+ A  A+ II+K+NP++ N+ +LL G+  TKDFR DRV LF NI G VV++
Sbjct: 48  KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQI 107

Query: 69  PTV 72
           P V
Sbjct: 108 PRV 110

[Close]

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.4e-12
Identity = 31/63 (49.21%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 9   KNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 68
           K  WPEL+G+   +A+ II+K+NP++ N+ +LL+G+  T D+  DRV LF NI G VV+M
Sbjct: 48  KQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQM 107

Query: 69  PTV 72
           P V
Sbjct: 108 PVV 110

[Close]

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-11
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 9   KNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 68
           K +WPEL+G+   +A+ II+K+N  + NV +LL G+  T D R DRV LF NI G VV++
Sbjct: 44  KLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDI 103

Query: 69  PTVG 73
           P VG
Sbjct: 104 PVVG 107

[Close]

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 4.5e-11
Identity = 30/63 (47.62%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 9   KNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 68
           K +WPEL+G+   +A+ II+K+N  + NV++LL G+  T D+R +RV LF NI G VV++
Sbjct: 44  KQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQI 103

Query: 69  PTV 72
           P V
Sbjct: 104 PRV 106

[Close]

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot
Match: P01052 (Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.9e-11
Identity = 31/63 (49.21%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 9  KNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 68
          K QWPEL+G+   +A+ II+K N  + NV +LL G+  T DFR +RV LF +I G VV++
Sbjct: 8  KLQWPELIGVPTKLAKEIIEKQNSLISNVHILLNGSPVTMDFRCNRVRLFDDILGSVVQI 67

Query: 69 PTV 72
          P V
Sbjct: 68 PRV 70

[Close]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 8.7e-34
Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENV VLLAGTGTTKDFRSDRVWLFTN
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IDGRVVEMPTVG 73
          IDGRVVEMPTVG
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.3e-25
Identity = 57/72 (79.17%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADIC P KNQWPELVGIKAT AEYII+KDNP+VE V VLLAGTGTTKD R DRVWLF N
Sbjct: 1  MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 61 IDGRVVEMPTVG 73
          I+ +VVE+P VG
Sbjct: 61 IEEKVVEIPKVG 72

[Close]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.8e-24
Identity = 55/72 (76.39%), Postives = 61/72 (84.72%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADIC P KNQWPELVG+KAT A+YIIKKDNPNVENV VLLAG+GTT+D R DRVW+F N
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IDGRVVEMPTVG 73
          I   VVE+P VG
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.7e-13
Identity = 40/74 (54.05%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAG--TGTTKDFRSDRVWLF 60
          MAD C P KN+WPELVGI+A IAE II+K+NPNV+ V++ L G  +G T +F   RV L 
Sbjct: 1  MADFCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLL 60

Query: 61 TNIDGRVVEMPTVG 73
           NI+G +V +P++G
Sbjct: 61 VNINGVIVGIPSIG 74

[Close]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL
Match: A0A4Y7LG33 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046386 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.2e-13
Identity = 41/72 (56.94%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MA  C P K+ WPELVG     A  II+++ PNV N  VLL GT TT DFR DRVW+F N
Sbjct: 1  MASECGPGKSSWPELVGFDGETAARIIEREIPNV-NAIVLLEGTPTTLDFRCDRVWVFVN 60

Query: 61 IDGRVVEMPTVG 73
            G+VVE PT+G
Sbjct: 61 EAGKVVETPTIG 71

[Close]

BLAST of CSPI05G12330 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 152.1 bits (383), Expect = 1.8e-33
Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENV VLLAGTGTTKDFRSDRVWLFTN
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IDGRVVEMPTVG 73
          IDGRVVEMPTVG
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of CSPI05G12330 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 123.6 bits (309), Expect = 6.9e-25
Identity = 57/72 (79.17%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADIC P KNQWPELVGIKAT AEYII+KDNP+VE V VLLAGTGTTKD R DRVWLF N
Sbjct: 1  MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 61 IDGRVVEMPTVG 73
          I+ +VVE+P VG
Sbjct: 61 IEEKVVEIPKVG 72

[Close]

BLAST of CSPI05G12330 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 120.6 bits (301), Expect = 5.8e-24
Identity = 55/72 (76.39%), Postives = 61/72 (84.72%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MADIC P KNQWPELVG+KAT A+YIIKKDNPNVENV VLLAG+GTT+D R DRVW+F N
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IDGRVVEMPTVG 73
          I   VVE+P VG
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of CSPI05G12330 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104.4 bits (259), Expect = 4.3e-19
Identity = 45/72 (62.50%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFTN 60
          MAD C P KN+WPELVGIK+TIAE+II+KDN NVE+V  +LAG+  T+D R DRV +F N
Sbjct: 1  MADFCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVN 60

Query: 61 IDGRVVEMPTVG 73
          I G +V++PT+G
Sbjct: 61 IKGIIVQIPTIG 72

[Close]

BLAST of CSPI05G12330 vs. NCBI nr
Match: XP_022963567.1 (inhibitor of trypsin and hageman factor-like [Cucurbita moschata])

HSP 1 Score: 84.7 bits (208), Expect = 3.5e-13
Identity = 40/74 (54.05%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAG--TGTTKDFRSDRVWLF 60
          MAD C P KN+WPELVGI+A IAE II+K+NPNV+ V++ L G  +G T +F   RV L 
Sbjct: 1  MADFCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLL 60

Query: 61 TNIDGRVVEMPTVG 73
           NI+G +V +P++G
Sbjct: 61 VNINGVIVGIPSIG 74

[Close]

BLAST of CSPI05G12330 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.5e-09
Identity = 29/73 (39.73%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 1  MADICR-PEKNQWPELVGIKATIAEYIIKKDNPNVENVRVLLAGTGTTKDFRSDRVWLFT 60
          MA IC  P K+ WPEL+G K   A+ +I+++NP ++ V ++L GT   + F   RV+++ 
Sbjct: 28 MASICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWV 87

Query: 61 NIDGRVVEMPTVG 73
          N  G VV++P +G
Sbjct: 88 NDCGIVVQIPIIG 99

The following BLAST results are available for this feature:

BLAST of CSPI05G12330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	2.2e-13	53.97	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P05118	2.4e-12	49.21	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
Q00783	1.2e-11	50.00	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P08454	4.5e-11	47.62	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]
P01052	5.9e-11	49.21	Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 ...	[more]

BLAST of CSPI05G12330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMM0	8.7e-34	98.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A0M3SAG8	3.3e-25	79.17	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KME7	2.8e-24	76.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A6J1HFK9	1.7e-13	54.05	inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A4Y7LG33	2.2e-13	56.94	Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046386 PE=3 SV=1	[more]

BLAST of CSPI05G12330 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN50838.1	1.8e-33	98.61	hypothetical protein Csa_004692 [Cucumis sativus]	[more]
ALD47589.1	6.9e-25	79.17	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50840.1	5.8e-24	76.39	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
KAG6571494.1	4.3e-19	62.50	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022963567.1	3.5e-13	54.05	inhibitor of trypsin and hageman factor-like [Cucurbita moschata]	[more]

BLAST of CSPI05G12330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	1.5e-09	39.73	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 11..24 score: 47.52 coord: 37..48 score: 32.61 coord: 49..59 score: 49.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..72 e-value: 8.8E-23 score: 80.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 6..72
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 12..23
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 5..72 e-value: 4.5E-22 score: 80.2
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 6..72
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 6..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G12330.1	CSPI05G12330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity