CSPI05G11750 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G11750
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncytochrome P450 714A1-like
LocationChr5: 10399480 .. 10404283 (+)
RNA-Seq ExpressionCSPI05G11750
SyntenyCSPI05G11750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAGATTACAAGACGGAAGTTCATAAGGAGAAGGAGAAGGAGAAGGAGAAGGGTTAAAGATTTTCATTACGCTATGGAAGGGTATGTAATGAGTGGTTTTGTGTTGGTTTTTCCGACCGCGATTGTGGTAGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGAAGCACAAGGCGTGACTGGACCGCCCCCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGGAAGCAATATGGTATTTTTGTTAATTTCTATCCTTTTGTATTTTTGGGTTAAATTATAACGTAGTCCAGGTTGATAGAAGTTCTGTCTAACTAAATAGTTTGACAATTCTTTTTTAAATTTTTGAAATTTTCTTATTTGTTTCACGCAATTTCATACAAATTTAATTTTATGAAATGTTACATTTAATTGAGAGTATGCGTTGTATGTTGAGTGAGTGGACCTTTGAAAAAATAATGAAGTAATTTTATATGCAATCCAAACTTCTAGGAATAATATAATTTAAACAAGAAAATCTCCTCCATGGTAATGGTATACACACACATATATATTTTATTGTTTTCAGACAATATTATAATTTTATACGAGTAGTGGAGATTTTAATATTCTGACTACAAATAAATATTATTGTTGCCTCAATCATTCCACCGTCTAAAAGATTGAACAAAATTTATTACACGTTTTGAAAATTCAATATTAGTTTTTTCTCTTTTTTTTTAAAAAATAAAATTTAAATAGATAAAAGTAGTTTTCATGAATAGTATCAAAAGTCAATTCTCATAATCTCGACTTCAACCCAACTTCCTGTCCATTAAATAGTGAATAGTACTAAATACACATTTTATTTTAATTAATGCGTTTACTATTTTTAATCACATCTCCATCTTATTTTTTTGGATCAAAGGTTTAATTATATGAGTTTTAATAACATAAATATTTCATCTCAAAAATAAAAATCCTTTGATAACATTAGAAGAAGTTCGTTTCTGATATAAAATTTCTCCACTTCTAATGGATATTCTCAACAAACAAAAATCATTAAAATATATATATATATTTATTTATTTAATTAAATTCATTTTATAACATTAATCTTTTTTGTTTTTCATTGCCACAATATATCTGCTCTATCCAAAATTATAACCAGAATGCATACAAAACTTCAAAGTACTTTTAAACTCTCTCACACAATCCAAAACAGTTGGCTACATTAGAATTGTCCTTTTCAGTAATTTGTCCTACGTGCCTCTTTCGTTATAATACAAATAGATTAGGTTAAAATTTCACATTTTGAATTTTATACTTTTAAAATTACATTATTATAACCTTTAAACTTTTCAATTTTTTAAAAATTCTCACATATAATGGATAAAATATTTAGAATTCTTTTGTATTAGAGAAGAATGAATTTTTAAAAAAATTGAATTGGTTAAAAACTTATTAGACATACAGTTGATTAACTCTATATTTAATTACACAACTTAAAGTTTAATGTTTTTAATATTAGATAATTTTAAAAGTTGAGTGATAAATAAACTAAACAGTAGAGACCGAAACACGAGACGATGAAACACAAGAATGGGGCATGGATGAGTAAATAGGAGAGGCCCTATGAATTGAATGTAGAGAAACTTTGAGATGTTGTTTGTTTGGTTTGGCAGGGCCATTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAATCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGTAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTGGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATTTCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTTCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGGTTATTTTCTCTCCTTTTTCTTTCTTCACAACTAATTTTATTAATACTAACAAAAAAAAAAAAAAAAACTTTAAAATTACTTTAAAAGAAAATTGGATTGAATCTGAGTCAGCTGAGTCAGCTGCCTACACACCAGCATAAATGTCTACGTACATTTTGATGTGTCACATTTGTATTGGCTTATTAGATCACTCATTGAATGCAATAGAATACTATTGAAACATGTTCTAAATATCTTCCACCATAATTAATTCTTTTATAAAATATTTTTCTAATACACTAAAATAAACTTTGAATAAAAACTTTTTGGATGCATATTTAAGTGTATATATAGCTATATATTTTATACATTATCTTTGATCACTTAAACTTTTTTCTAATCTATCTGAAAGTTATTCAAAAATAGTTTTAAACTAATTCAACATTTTCATTGTTTTTATTTACTAAGATAAGATTGTTTTAAAAAAATATTTTATCAGGGAATTTGACAACTTTAATAATTTGGAAGAGTTGGGTCAGTCCTTTCTTTGATTCATACTAAAATATTCATATTTTTTACATTCTTATTTTTGTCAACTTCTTACAACTCTTTATTTTCGTTACTAATCAAATGACTCCTAATAAAGTAAATGTACCAAATGTGGGTTAAAGTATTATTATCATTTTTTGAAAAATAGTGGTTAAAAATTGATTTTATTAATTAGAATCACTTTATATATATTTGAATAATAAAAATAAGCCAATAAATTAAAAAAAGAAAAGAAAAAGAGAAGAAGAATCTCTTTAGACTGATAATAATTGAGGAGCTGAATTGTGTTTAAATTGACAATAGCAATAAGATTAATAAAGGTTTTAATTTGATGAACAGTGATAGATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAAAGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCCAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCCACTTCACAACTCAAATCGGTAATCTCTCTCTCAAAGACATAGTTTGTTTTAGATTGTACAATAATATGTGATTTTGCAGGAGTAAAACTTGTTTAATTAGGTTTATTTCTCCAAATATTAGAAATTATTATAGTGTTGCATTTTTTAAAAATTGTAAATATAGCAAAATTTATCGGTTAGATTTTATCGTTGATAAACTTTAACAATTTTGCTACATTTACCATTTTTTTAAAAAAATGTTACCTATGCGAATATTTTAAATCTATACTTCTATGCATATACTAATCTAATTGTTTTATATGTAATTATCATTTTCTTTTAAGTTATATAGAAAGTTAAATGAGTTAAGAGCACGATGTTTTATAGTTATGAAGTTTTCGAACTTTTGGAACTAATAAGTGGATCACTTACTTCTTGACTCTAACATCTACTTTTTAAATAATAGGTCTTTAAAACTTTTTGCCGAGAAAAATAAGATAATAAAAAATGATGAATTTTAAGATGATGATAAAGAAAGGAAGGGTATGGGTGGAATCTAAGTTTTGAGGAGAATGGACTGAATTGTTGCAAATTTAAAATACTAACTAATTGTAAACCAAAAGTAAATGGTGGACACATATGTATACTCTCCTAACCTTAGCACATGAAACAACTGGTGTATGTAGGTGAGTATGGTGATTCACGAAACGCTACGATTGTATCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGCGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTTGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGGGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAATTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTTTGAATTGAGGTTTAAGAACTTTGTAAATGAGGTTAATTTAGCTTAGGGTTATTGCTGGGTCAAGTTATAAGAAATTGGAGTGATATGTTTGGTATGTGTGTTTATTTATCATTTGGTACAAGTTTAATTTGTTAGTTATACAAGGCTACAGTACAAATATACATTATGGAAATCACATTTGATTCACTGTACTACAAGC

mRNA sequence

AGAAGATTACAAGACGGAAGTTCATAAGGAGAAGGAGAAGGAGAAGGAGAAGGGTTAAAGATTTTCATTACGCTATGGAAGGGTATGTAATGAGTGGTTTTGTGTTGGTTTTTCCGACCGCGATTGTGGTAGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGAAGCACAAGGCGTGACTGGACCGCCCCCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGGAAGCAATATGGGCCATTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAATCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGTAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTGGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATTTCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTTCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGTGATAGATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAAAGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCCAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCCACTTCACAACTCAAATCGGTGAGTATGGTGATTCACGAAACGCTACGATTGTATCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGCGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTTGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGGGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAATTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTTTGAATTGAGGTTTAAGAACTTTGTAAATGAGGTTAATTTAGCTTAGGGTTATTGCTGGGTCAAGTTATAAGAAATTGGAGTGATATGTTTGGTATGTGTGTTTATTTATCATTTGGTACAAGTTTAATTTGTTAGTTATACAAGGCTACAGTACAAATATACATTATGGAAATCACATTTGATTCACTGTACTACAAGC

Coding sequence (CDS)

ATGGAAGGGTATGTAATGAGTGGTTTTGTGTTGGTTTTTCCGACCGCGATTGTGGTAGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGAAGCACAAGGCGTGACTGGACCGCCCCCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGGAAGCAATATGGGCCATTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAATCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGTAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTGGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATTTCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTTCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGTGATAGATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAAAGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCCAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCCACTTCACAACTCAAATCGGTGAGTATGGTGATTCACGAAACGCTACGATTGTATCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGCGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTTGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGGGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAATTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTTTGA

Protein sequence

MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL*
Homology
BLAST of CSPI05G11750 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.8e-165
Identity = 297/519 (57.23%), Postives = 378/519 (72.83%), Query Frame = 0

Query: 17  IVVAVVAILLHFYS---YGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           +V+ V+ + +  Y       WR    +RRKL  QGV GPPPS+  GN+PEMQKIQ Q  +
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWR----MRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMS 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
             S  +    I+AHDYTS+LFPY   WRKQYG +YTYSTG +QHLY N  ELVK+L+ +N
Sbjct: 76  -NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQAN 135

Query: 137 NLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
            LN+GK  YVTK+L  ILG+ V+ SNG  WA QR++IAPEFF+D+V+ M   +V++A  +
Sbjct: 136 TLNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPM 195

Query: 197 INKWE---SRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           ++KWE    R G+   +I VDEDLR  SADVISRACFGSS+ KGKEIFSKLR LQK I  
Sbjct: 196 LSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 DSFLFGYSSWSDRFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
           ++ LF  + ++D     +KH   +I+ LE+ IESLIWETV++R++EC       KDL+QL
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 IMEATID--DPNIGAKDSS-KNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 376
           I+E      D N+  K  S K+F+VDNCKSIYFAGHE++AVA +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 436
           R E    C +G  D  + S LK+V+MVI ETLRLYPPAAFV+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 496
           C+WTLIPTLHR+ EIWG DANEF PERF+ GV+KACK PQ++VPFG G RLCLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           ELK+++SLIVS+F F+LSP Y H P +RM+VEP +GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of CSPI05G11750 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.3e-154
Identity = 268/518 (51.74%), Postives = 367/518 (70.85%), Query Frame = 0

Query: 17  IVVAVVAILLHFYSYGA---WRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           ++V + ++  H Y       WR    +RR L+ QGV GPPPSI  GN+ EMQ+IQ +   
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWR----MRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKH 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
            +        I++HDY+S+LFP+F  WRKQYG +YTYSTG +QHLY N  E+VK+LS +N
Sbjct: 76  CS-----GDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTN 135

Query: 137 NLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
            LN+G+  ++TK+L PILG  ++ SNG  WA QR++IA EF  D+++ M   MV++A  +
Sbjct: 136 TLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPM 195

Query: 197 INKWESRV---GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           +NKWE  V   G+   +I VDEDL+  SADVI++ACFGSS+ KGK IFS +RDL   I +
Sbjct: 196 LNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITK 255

Query: 257 DSFLFGYSSWSDRFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
            S LF ++ ++D     +KH    I+ LE E+ES IWETV++R+ EC  T    KDL+QL
Sbjct: 256 RSVLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHK--KDLMQL 315

Query: 317 IMEATID--DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIR 376
           I+E  +   D N+  K + + F+VDNCKSIYFAGH+STAV+ +W LMLLAL+P WQ +IR
Sbjct: 316 ILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIR 375

Query: 377 SEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 436
            E   +C +G  D  +   LK+V+MVI ET+RLYPPA  V RE   + +LG++VVPKGVC
Sbjct: 376 DEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVC 435

Query: 437 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 496
           +WTLIP LHR+ EIWG DAN+FKPERF+ G++KACK+PQ+Y+PFG GPR C+GKNF ++E
Sbjct: 436 IWTLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMME 495

Query: 497 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           +K+++SLIVSKF F+LSP Y H PS++++VEP +GV I
Sbjct: 496 VKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of CSPI05G11750 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.4e-132
Identity = 239/512 (46.68%), Postives = 334/512 (65.23%), Query Frame = 0

Query: 21  VVAILLHFYSY-GAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAAMASPLH 80
           ++ ILL  Y Y   W R   +R+KL +QGV GP PS L+GN+PEM++IQ    +      
Sbjct: 17  ILCILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEA 76

Query: 81  RASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNNLNVGK 140
            ++ + + +Y +TLFPYF+ W + YG +Y YSTG+ Q L      +VK+L+   +L++GK
Sbjct: 77  GSTDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDLGK 136

Query: 141 PFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWES 200
           P Y+ K+   +LG  ++ SNG +W  QRKVIAPE FM+RV+ M   M++AA  ++N W++
Sbjct: 137 PCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKN 196

Query: 201 RVGD--GTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGY 260
            V D  G+ EI VDE LR FSADVISRACFGSS+ +GKEIF K+R LQK + + S L G 
Sbjct: 197 EVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 256

Query: 261 SSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQLIMEATIDDP 320
                R+L  R ++ I  L+  I +LI      ++ E   ++S +KDLL  I++ + D P
Sbjct: 257 P--GSRYLPTRSNRGIWNLDSSIRTLILNI--SKKYEHDSSTSVNKDLLHSIIQGSKDGP 316

Query: 321 NIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQACPDGH 380
              A  + ++FIVDNCK+IYFAGHE+T+  A W LMLLA H EWQ R R E    C    
Sbjct: 317 --FASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRP 376

Query: 381 LDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHRE 440
           LD     +LK ++MVI ETLRLYPPA+FVARE   + +LG + +PKG  +W  I   HR+
Sbjct: 377 LDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRD 436

Query: 441 VEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSK 500
             +WG  A++F P+RFANG+A ACK P  Y+PFG G R C G+N A+VELK+++SL++SK
Sbjct: 437 PSVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSK 496

Query: 501 FRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           F F LSP Y HCP++R+ +EP  GV ++F+ L
Sbjct: 497 FEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of CSPI05G11750 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 7.5e-124
Identity = 253/566 (44.70%), Postives = 340/566 (60.07%), Query Frame = 0

Query: 6   MSGFVLVFPTAI--VVAVVAILLHFYSYGAW----RRTAEVRRKLEAQGVTGPPP-SILY 65
           M  F + F  A   VV   A++       AW    RR AEV R+   QG+ GPPP S L 
Sbjct: 1   MESFFVFFTAAALPVVVAAAVIAGLCITAAWLARPRRVAEVFRR---QGIDGPPPSSFLA 60

Query: 66  GNLPEMQKIQLQTAAMASPLHRASAIVA---------------HDYTSTLFPYFVEWRKQ 125
           GNLPEM+      A+ A+P        +                DY + +FPYF +WRK 
Sbjct: 61  GNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWRKA 120

Query: 126 YGPLYTYSTGTRQHLYANKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVW 185
           YG  Y Y    R  LY    EL+ ++    +L++GKP Y+ K   P+ G  V+++NGA W
Sbjct: 121 YGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKGQEPLFGGGVLKANGACW 180

Query: 186 AVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWESRV----GDGTIEIDVDEDLRGFSA 245
           A QRKVIAPEF+M RVRAM   MVDAA  LI  WESR+    G    E+ VD DLR FS 
Sbjct: 181 ARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSFSF 240

Query: 246 DVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKE 305
           DVISRACFGS Y +G+EIF +LR+L  L+ E S +F   S   R L   K++RI RL  E
Sbjct: 241 DVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSL--RHLPTGKNRRIWRLTGE 300

Query: 306 IESLIWETVQQRQ----------KECSKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFI 365
           I SLI E V++R+           + +  S  ++D L  I+E      N G +    +F+
Sbjct: 301 IRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIE------NSGGQPRPDDFV 360

Query: 366 VDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQAC-PDGHLDPTA-----T 425
           VDNCK+IYFAGHE++AV ATW LMLLA HPEWQDR R+E  + C  DG   P A      
Sbjct: 361 VDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDMV 420

Query: 426 SQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGE 485
           S++++V MV+ ETLRL+PP++FV RETF + QLG ++ PKG  L+  + T+H +V  WG 
Sbjct: 421 SRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWGP 480

Query: 486 DANEFKPERFANGVAKACKFPQA-YVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSL 529
            A  F P RF +GVA ACK PQA ++PFG G R CLG+N ALVE+K ++++++++F F+L
Sbjct: 481 TARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEFTL 540

BLAST of CSPI05G11750 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.6e-118
Identity = 229/534 (42.88%), Postives = 329/534 (61.61%), Query Frame = 0

Query: 6   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 65
           M+  V V  +   V    + ++ Y +  W    +V  K E Q + GP PS  YGNL +M+
Sbjct: 5   MAMAVKVLLSLCCVGACGLAVYLY-HILWLVPQKVLAKFEDQKIGGPRPSFPYGNLADMR 64

Query: 66  KIQLQTAAMASPLHRASAI--------VAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQH 125
           +     AA A+   RASA         + HDY   + PY+ +WRK+YGP++TYS G    
Sbjct: 65  E-----AAAAAKAARASARRSGSGGGGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVF 124

Query: 126 LYANKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMD 185
           L+ ++ ++V+D++L  +L++GK  Y+     P+ G  +++SNG  W  QRK+IAPEFF+D
Sbjct: 125 LHVSRPDVVRDINLCVSLDLGKSSYLKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLD 184

Query: 186 RVRAMAFHMVDAASCLINKWESRV--GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGK 245
           +V+ M   MVD+A  L+  WE RV    G  +I +D+D+R +SADVISR CFGSSY KGK
Sbjct: 185 KVKGMVDLMVDSAQPLLMSWEERVDRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGK 244

Query: 246 EIFSKLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKEC 305
           EIF K+R+LQ+ + + + L   +    RF    ++K+   L K++  LI E V       
Sbjct: 245 EIFMKIRELQQAVSKPNVLAEMTGL--RFFPSMRNKQAWELHKQVRKLILEIV------- 304

Query: 306 SKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLL 365
            K S  D++LL  I+ +      +G  + ++NFIVDNCKSIYFAGHESTAV A W LMLL
Sbjct: 305 -KESGEDRNLLSAILHSA-STSRVGIAE-AENFIVDNCKSIYFAGHESTAVTAAWCLMLL 364

Query: 366 ALHPEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQ 425
            LHPEWQ+R+R E  + C    +D  +  ++K+++MVI ETLRLYP  AFV+R+   E +
Sbjct: 365 GLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNLTMVIQETLRLYPAGAFVSRQALQELK 424

Query: 426 LGNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPR 485
           LG V +PKGV ++  + T+H + E+WG D  EF PERF++   +      +Y+PFGAG R
Sbjct: 425 LGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFNPERFSDVRPQL----HSYLPFGAGAR 484

Query: 486 LCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
            CLG+ FA+ ELKI+ISLIVSKF   LSP Y H P+ ++IVEP  GV +   ++
Sbjct: 485 TCLGQGFAMAELKILISLIVSKFVLKLSPHYQHSPTLKLIVEPELGVDLTLTKV 516

BLAST of CSPI05G11750 vs. ExPASy TrEMBL
Match: A0A0A0KQQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 1.8e-306
Identity = 524/524 (100.00%), Postives = 524/524 (100.00%), Query Frame = 0

Query: 6   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 65
           MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ
Sbjct: 1   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 60

Query: 66  KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL 125
           KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL
Sbjct: 61  KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL 120

Query: 126 VKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH 185
           VKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH
Sbjct: 121 VKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH 180

Query: 186 MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ 245
           MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ
Sbjct: 181 MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ 240

Query: 246 KLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL 305
           KLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL
Sbjct: 241 KLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL 300

Query: 306 LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 365
           LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI
Sbjct: 301 LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 360

Query: 366 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 425
           RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV
Sbjct: 361 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 420

Query: 426 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 485
           CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV
Sbjct: 421 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 480

Query: 486 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL
Sbjct: 481 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 524

BLAST of CSPI05G11750 vs. ExPASy TrEMBL
Match: A0A1S3CNV5 (cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.7e-296
Identity = 504/529 (95.27%), Postives = 517/529 (97.73%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEGYV+SGFVLVFPTA+V+AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSWSDRFL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of CSPI05G11750 vs. ExPASy TrEMBL
Match: A0A5A7VH75 (Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00950 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.9e-294
Identity = 502/529 (94.90%), Postives = 515/529 (97.35%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEGYV+SGFVLVFPTA+V+AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSW  RFL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSW--RFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of CSPI05G11750 vs. ExPASy TrEMBL
Match: A0A6J1K5V5 (cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.5e-239
Identity = 416/530 (78.49%), Postives = 464/530 (87.55%), Query Frame = 0

Query: 6   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 65
           M G   V   A+ VAVVA+++HFY YG WRRTAE+RRKL AQGVTGPPPS+LYGNL EMQ
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFY-YGVWRRTAEIRRKLRAQGVTGPPPSLLYGNLQEMQ 60

Query: 66  KIQLQ-TAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVE 125
           +IQL+  AAM SP H AS IVAHDYTSTLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V+
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAF 185
           LVKDLSL+N L++GKP YVTKKLAPILG S+VR+NG VWA+QRK+IAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 HMVDAASCLINKWESRVGDG---TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKL 245
            M +AA+ ++ KWE R+G+G   T EIDVDEDLR FSADVISRACFGSSYEKGK IFSKL
Sbjct: 181 LMTEAAAAMLQKWEGRIGEGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSKL 240

Query: 246 RDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSS 305
           R LQKL+ E +FLFGY S SDR  Q  KH+RI +LEKEIESLIWETVQQRQKECSKT SS
Sbjct: 241 RVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKT-SS 300

Query: 306 DKDLLQLIMEATIDDPNIGA--KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 365
           DKDLLQLIMEA ++DP + A   +S K FIVDNCKSIYFAGHESTAVAATWSLMLLALHP
Sbjct: 301 DKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360

Query: 366 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 425
           EWQDRIRSEF QAC DG ++ T  SQLKSVSMV+ ETLRLYPPAAFVARETF+ET+LGNV
Sbjct: 361 EWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGNV 420

Query: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 485
           V+PKG+ LWTLIPTLHREVEIWGE+ANEFKPERF NG+AKACKFPQAY+PFGAGPRLCLG
Sbjct: 421 VIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCLG 480

Query: 486 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           KNFALV+LKI+ISLIVSKFRFSLSPEY H PSYRMIVEPANG+KI+F+R+
Sbjct: 481 KNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of CSPI05G11750 vs. ExPASy TrEMBL
Match: A0A6J1FRZ8 (cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447915 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 3.3e-239
Identity = 419/531 (78.91%), Postives = 466/531 (87.76%), Query Frame = 0

Query: 6   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 65
           M G   V  TA+ VAVVA+++HFY YG WRRTAE+RRKL AQGVTGP PS+LYGNLPEMQ
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFY-YGVWRRTAEIRRKLRAQGVTGPSPSLLYGNLPEMQ 60

Query: 66  KIQLQ--TAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKV 125
           +IQL+   AAMASP H AS IVAHDYTSTLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 ELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMA 185
           +LVKDLSL+N+L++GKP YVTKKLAPILG S+VR+NG VWA+QRK+IAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 FHMVDAASCLINKWESRV--GDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 245
             M +AA+ ++ KWE R+  GDG T EIDVDE LR FSADVISRACFGSSYEKGK IFSK
Sbjct: 181 ALMTEAAAAMLQKWEERIDEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 305
           LR LQKL+ E +FLFGY S SDR  Q  KH+ I +LEKEIESLIWETVQQRQKECSKT S
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT-S 300

Query: 306 SDKDLLQLIMEATIDDPNIGA--KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALH 365
           SDKDLLQLIMEA ++DP +G+   +S K FIVDNCKSIYFAGHESTAVAATWSLMLLALH
Sbjct: 301 SDKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGN 425
           PEWQDRIRSEF QACPDG ++ TA SQLKS SMV+ ETLRLYPPAAFVARETF+ET+LGN
Sbjct: 361 PEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCL 485
           VV+PKGV LWTLIPTLHREVEIWGE+AN+FKPERF NG+AKACKFPQAYVPFGAGPRLCL
Sbjct: 421 VVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLCL 480

Query: 486 GKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           GKNFALV+LKI+ISLIVSKFRFSLS EY H PSYRMIVEPANG+KI+F+R+
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of CSPI05G11750 vs. NCBI nr
Match: XP_004146466.1 (cytochrome P450 714A1 [Cucumis sativus] >KAE8648060.1 hypothetical protein Csa_005795 [Cucumis sativus])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.7e-309
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN
Sbjct: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529

BLAST of CSPI05G11750 vs. NCBI nr
Match: XP_008465257.1 (PREDICTED: cytochrome P450 714A1-like [Cucumis melo])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.5e-296
Identity = 504/529 (95.27%), Postives = 517/529 (97.73%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEGYV+SGFVLVFPTA+V+AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSWSDRFL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of CSPI05G11750 vs. NCBI nr
Match: KAA0067078.1 (cytochrome P450 714A1-like protein [Cucumis melo var. makuwa] >TYK26315.1 cytochrome P450 714A1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 502/529 (94.90%), Postives = 515/529 (97.35%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEGYV+SGFVLVFPTA+V+AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSW  RFL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSW--RFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of CSPI05G11750 vs. NCBI nr
Match: XP_038893045.1 (cytochrome P450 714A1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 954.1 bits (2465), Expect = 5.0e-274
Identity = 469/530 (88.49%), Postives = 494/530 (93.21%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEG ++ GF+ VFP A+ V VVAIL+H Y YGAWRRTAEVRRKL AQGVTGPPPSILYGN
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPPSILYGN 65

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASP HRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 66  LPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 125

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLN+GKPFYVTKKLAPILG S+VRSNGA+WAVQRK+IAPEFFMDRVR
Sbjct: 126 NKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRVR 185

Query: 181 AMAFHMVDAASCLINKWESRVGDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           AMA  M DAAS L+ KWESR+GDG ++EI+VDEDLRGFSADVISRACFGSSYEKGKEIFS
Sbjct: 186 AMAVLMADAASSLVEKWESRIGDGASVEIEVDEDLRGFSADVISRACFGSSYEKGKEIFS 245

Query: 241 KLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
           KLRDLQKLI E SFLFGYSS SDRFLQPRKHKRI +LEKEIESLIWETVQQRQKE SKTS
Sbjct: 246 KLRDLQKLISEGSFLFGYSSLSDRFLQPRKHKRIKKLEKEIESLIWETVQQRQKEFSKTS 305

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
           SS+KDLLQLIMEAT +DPN+GA DSSKNFIVDNCKSIYFAGHESTAVAATW+LMLLALHP
Sbjct: 306 SSEKDLLQLIMEATTNDPNVGANDSSKNFIVDNCKSIYFAGHESTAVAATWTLMLLALHP 365

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IRSEFA ACPDG  D T  S LK+VSMV+HE LRLYPPAAFVARETFA+T+LGNV
Sbjct: 366 EWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADTRLGNV 425

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERF NG+AKACKFPQAYVPFGAGPRLCLG
Sbjct: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGPRLCLG 485

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           KNFALV+LKIIISLIVSKFRFSLSPEY HCPSYRMIVEPANGVKI FQRL
Sbjct: 486 KNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 535

BLAST of CSPI05G11750 vs. NCBI nr
Match: XP_038893046.1 (cytochrome P450 714A1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 945.3 bits (2442), Expect = 2.3e-271
Identity = 467/530 (88.11%), Postives = 492/530 (92.83%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           MEG ++ GF+ VFP A+ V VVAIL+H Y YGAWRRTAEVRRKL AQGVTGPPPSILYGN
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPPSILYGN 65

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASP HRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 66  LPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 125

Query: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSNNLN+GKPFYVTKKLAPILG S+VRSNGA+WAVQRK+IAPEFFMDRVR
Sbjct: 126 NKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRVR 185

Query: 181 AMAFHMVDAASCLINKWESRVGDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           AMA  M DAAS L+ KWESR+GDG ++EI+VDEDLRGFSADVISRACFGSSYEKGKEIFS
Sbjct: 186 AMAVLMADAASSLVEKWESRIGDGASVEIEVDEDLRGFSADVISRACFGSSYEKGKEIFS 245

Query: 241 KLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
           KLRDLQKLI E SFLFGYSS   RFLQPRKHKRI +LEKEIESLIWETVQQRQKE SKTS
Sbjct: 246 KLRDLQKLISEGSFLFGYSSL--RFLQPRKHKRIKKLEKEIESLIWETVQQRQKEFSKTS 305

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
           SS+KDLLQLIMEAT +DPN+GA DSSKNFIVDNCKSIYFAGHESTAVAATW+LMLLALHP
Sbjct: 306 SSEKDLLQLIMEATTNDPNVGANDSSKNFIVDNCKSIYFAGHESTAVAATWTLMLLALHP 365

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IRSEFA ACPDG  D T  S LK+VSMV+HE LRLYPPAAFVARETFA+T+LGNV
Sbjct: 366 EWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADTRLGNV 425

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERF NG+AKACKFPQAYVPFGAGPRLCLG
Sbjct: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGPRLCLG 485

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           KNFALV+LKIIISLIVSKFRFSLSPEY HCPSYRMIVEPANGVKI FQRL
Sbjct: 486 KNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 533

BLAST of CSPI05G11750 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 581.6 bits (1498), Expect = 6.2e-166
Identity = 297/519 (57.23%), Postives = 378/519 (72.83%), Query Frame = 0

Query: 17  IVVAVVAILLHFYS---YGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           +V+ V+ + +  Y       WR    +RRKL  QGV GPPPS+  GN+PEMQKIQ Q  +
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWR----MRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMS 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
             S  +    I+AHDYTS+LFPY   WRKQYG +YTYSTG +QHLY N  ELVK+L+ +N
Sbjct: 76  -NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQAN 135

Query: 137 NLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
            LN+GK  YVTK+L  ILG+ V+ SNG  WA QR++IAPEFF+D+V+ M   +V++A  +
Sbjct: 136 TLNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPM 195

Query: 197 INKWE---SRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           ++KWE    R G+   +I VDEDLR  SADVISRACFGSS+ KGKEIFSKLR LQK I  
Sbjct: 196 LSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 DSFLFGYSSWSDRFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
           ++ LF  + ++D     +KH   +I+ LE+ IESLIWETV++R++EC       KDL+QL
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 IMEATID--DPNIGAKDSS-KNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 376
           I+E      D N+  K  S K+F+VDNCKSIYFAGHE++AVA +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 436
           R E    C +G  D  + S LK+V+MVI ETLRLYPPAAFV+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 496
           C+WTLIPTLHR+ EIWG DANEF PERF+ GV+KACK PQ++VPFG G RLCLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           ELK+++SLIVS+F F+LSP Y H P +RM+VEP +GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of CSPI05G11750 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 545.8 bits (1405), Expect = 3.8e-155
Identity = 268/518 (51.74%), Postives = 367/518 (70.85%), Query Frame = 0

Query: 17  IVVAVVAILLHFYSYGA---WRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           ++V + ++  H Y       WR    +RR L+ QGV GPPPSI  GN+ EMQ+IQ +   
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWR----MRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKH 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
            +        I++HDY+S+LFP+F  WRKQYG +YTYSTG +QHLY N  E+VK+LS +N
Sbjct: 76  CS-----GDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTN 135

Query: 137 NLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
            LN+G+  ++TK+L PILG  ++ SNG  WA QR++IA EF  D+++ M   MV++A  +
Sbjct: 136 TLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPM 195

Query: 197 INKWESRV---GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           +NKWE  V   G+   +I VDEDL+  SADVI++ACFGSS+ KGK IFS +RDL   I +
Sbjct: 196 LNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITK 255

Query: 257 DSFLFGYSSWSDRFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
            S LF ++ ++D     +KH    I+ LE E+ES IWETV++R+ EC  T    KDL+QL
Sbjct: 256 RSVLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHK--KDLMQL 315

Query: 317 IMEATID--DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIR 376
           I+E  +   D N+  K + + F+VDNCKSIYFAGH+STAV+ +W LMLLAL+P WQ +IR
Sbjct: 316 ILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIR 375

Query: 377 SEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 436
            E   +C +G  D  +   LK+V+MVI ET+RLYPPA  V RE   + +LG++VVPKGVC
Sbjct: 376 DEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVC 435

Query: 437 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 496
           +WTLIP LHR+ EIWG DAN+FKPERF+ G++KACK+PQ+Y+PFG GPR C+GKNF ++E
Sbjct: 436 IWTLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMME 495

Query: 497 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           +K+++SLIVSKF F+LSP Y H PS++++VEP +GV I
Sbjct: 496 VKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of CSPI05G11750 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 345.9 bits (886), Expect = 5.8e-95
Identity = 182/523 (34.80%), Postives = 301/523 (57.55%), Query Frame = 0

Query: 12  VFPTAIVVAVVAILLHFYSYGA--WRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQKIQL 71
           +  + +V+ V  IL   Y   +  W     +++ +E QGVTGP P  L GN+ E+  +  
Sbjct: 5   ILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVS 64

Query: 72  QTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDL 131
           Q+A+      +    + HD    L P++V W KQYG  +    GT   L   + EL+K+L
Sbjct: 65  QSAS------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKEL 124

Query: 132 SLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDA 191
            + +N   G+ +   +     +G+ ++ +NG  W  QR + AP F  +R++  A HMV+ 
Sbjct: 125 LMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVEC 184

Query: 192 ASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLIC 251
            S L+ +    VG+G  E+++ E++   +AD+ISR  FGSS+EKGKE+F+ L  LQ+   
Sbjct: 185 TSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCA 244

Query: 252 EDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQ--KECSKTSSSDKDLLQ 311
           + +    +     RFL  + ++ I  L+KE+E L+ E +Q R+   E  ++S+   DLL 
Sbjct: 245 QATRHLCFP--GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLG 304

Query: 312 LIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRS 371
           L++     D N    +++   I+D CK+ +FAGHE+TA+  TW+ MLLA +P WQ+++R 
Sbjct: 305 LLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVRE 364

Query: 372 EFAQAC-PDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 431
           E  +    +G       S+L S+S VI+E+LRLYPPA  + R  F + +LG++ +PKG+ 
Sbjct: 365 EVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLS 424

Query: 432 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 491
           +W  +  +H   E+WG+DAN+F PERF     +     + ++PF AGPR C+G+ FAL+E
Sbjct: 425 IWIPVLAIHHSEELWGKDANQFNPERFG---GRPFASGRHFIPFAAGPRNCIGQQFALME 484

Query: 492 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
            KII++ ++SKF F++S  Y H P   + ++P  GV+++ + L
Sbjct: 485 AKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of CSPI05G11750 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 340.5 bits (872), Expect = 2.4e-93
Identity = 184/529 (34.78%), Postives = 302/529 (57.09%), Query Frame = 0

Query: 5   VMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEM 64
           +M   VL +   IV+ ++  +L+      +     +++ +E QG+TGP P +L GN+ ++
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 65  QKIQLQTAAM-ASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKV 124
            K+   +A+   S +H       H+    L P++V W KQYG  +    GT   L   + 
Sbjct: 61  SKMLSHSASNDCSSIH-------HNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTET 120

Query: 125 ELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMA 184
           E++K+L   +N   GK +   +     +G+ ++ +NG  W  QR + AP F  DR++  A
Sbjct: 121 EMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYA 180

Query: 185 FHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRD 244
            HMV+    +  +    VG+   E+++ E++R  +AD+ISR  FGSS +KGKE+FS L  
Sbjct: 181 KHMVECTKMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTV 240

Query: 245 LQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQK--ECSKTSSS 304
           LQ+L  + +    +     RFL  + ++ I  L+ E+E L+ E +  R+   E  ++SS 
Sbjct: 241 LQRLCAQATRHLCFP--GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSY 300

Query: 305 DKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEW 364
             DLL L++     + N    + +   I+D CK+ +F GHE+T++  TW+LMLLA +P W
Sbjct: 301 GDDLLGLLLNQMDSNKN----NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTW 360

Query: 365 QDRIRSEFAQAC-PDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 424
           QD +R E  Q C  DG       S L S++ VI+E+LRLYPPA  + R  F + +LG+++
Sbjct: 361 QDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLI 420

Query: 425 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 484
           +PKG+ +W  +  +H   E+WGEDANEF PERF     ++    + ++PF AGPR C+G+
Sbjct: 421 IPKGLSIWIPVLAIHHSNELWGEDANEFNPERF---TTRSFASSRHFMPFAAGPRNCIGQ 480

Query: 485 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
            FA++E KII++++VSKF F++S  Y H P   + ++P  GV++V + L
Sbjct: 481 TFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of CSPI05G11750 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 322.8 bits (826), Expect = 5.2e-88
Identity = 168/530 (31.70%), Postives = 283/530 (53.40%), Query Frame = 0

Query: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60
           ME + MS    +F    VV +  + L  +    W      ++KL   G  GP PS  +GN
Sbjct: 1   MEFFEMSRVWYIFFKVFVVVICLMFLKLF-LRCWIWPVRAQKKLRGNGFVGPAPSFPFGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           L +M+K+++  A++     ++S I+ HD  S   P+F  W+++YG ++ Y  G    +Y 
Sbjct: 61  LNDMKKLKM--ASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYV 120

Query: 121 NKVELVKDLSLS-NNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRV 180
              E +  +S      + GKP    K   P+ G  +V   G  W   R +I P F    +
Sbjct: 121 ADPEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNL 180

Query: 181 RAMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           + M   MV++ S ++++W  ++  G  E D++ ++ G + ++I++  FG + E G ++  
Sbjct: 181 KVMTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLK 240

Query: 241 KLRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
            LR +Q  +   +   G        L  ++  +   L  EI+ L+   + +R+   ++  
Sbjct: 241 NLRAVQFALFNSNRYVGVP--FSNILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGD 300

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
               DLL ++++A     N  AK+     +VD CK+ +FAGHE+TA+A TW+ MLLA+HP
Sbjct: 301 DQGHDLLGMLLKAD-QKGNFTAKE-----LVDECKTFFFAGHETTALALTWTFMLLAIHP 360

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IR E  +   D  ++    + LK +S V++E LRLYPPA    R+   + ++   
Sbjct: 361 EWQDTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGR 420

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           V+P G  +W  +  +H +VE+WG+D NEFKPERF   +   CK    Y+PFG G R+C+G
Sbjct: 421 VIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIG 480

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           +N   +E KI++SL++S+F  S+SP Y H P+Y + + P  G+ ++ + L
Sbjct: 481 RNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z798.8e-16557.23Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1[more]
Q6NKZ85.3e-15451.74Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1[more]
Q2QYH74.4e-13246.68Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q5KQH77.5e-12444.70Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ... [more]
B6SSW81.6e-11842.88Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQQ71.8e-306100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1[more]
A0A1S3CNV51.7e-29695.27cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1[more]
A0A5A7VH757.9e-29494.90Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1K5V52.5e-23978.49cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1[more]
A0A6J1FRZ83.3e-23978.91cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
Match NameE-valueIdentityDescription
XP_004146466.13.7e-309100.00cytochrome P450 714A1 [Cucumis sativus] >KAE8648060.1 hypothetical protein Csa_0... [more]
XP_008465257.13.5e-29695.27PREDICTED: cytochrome P450 714A1-like [Cucumis melo][more]
KAA0067078.11.6e-29394.90cytochrome P450 714A1-like protein [Cucumis melo var. makuwa] >TYK26315.1 cytoch... [more]
XP_038893045.15.0e-27488.49cytochrome P450 714A1-like isoform X1 [Benincasa hispida][more]
XP_038893046.12.3e-27188.11cytochrome P450 714A1-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G24910.16.2e-16657.23cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.13.8e-15551.74cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
AT5G38450.15.8e-9534.80cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.12.4e-9334.78cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G52400.15.2e-8831.70cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..293
NoneNo IPR availablePANTHERPTHR24282:SF36CYTOCHROME P450 714A1-RELATEDcoord: 16..524
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 16..524
IPR001128Cytochrome P450PRINTSPR00385P450coord: 391..402
score: 49.19
coord: 339..356
score: 34.07
coord: 477..488
score: 45.17
coord: 468..477
score: 59.73
IPR001128Cytochrome P450PFAMPF00067p450coord: 92..524
e-value: 3.1E-77
score: 260.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 217..235
score: 23.31
coord: 101..120
score: 24.36
coord: 348..374
score: 25.36
coord: 467..477
score: 52.59
coord: 477..500
score: 28.89
coord: 328..345
score: 22.84
coord: 390..408
score: 27.16
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 34..529
e-value: 1.8E-104
score: 352.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 88..527
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 470..479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G11750.1CSPI05G11750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen