CSPI05G08550 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G08550
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr5: 7262823 .. 7264646 (+)
RNA-Seq Expression	CSPI05G08550
Synteny	CSPI05G08550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAAAAATGAGATTGGATTGGGCCCAAAAAGAGAGCCCAACAGTTGAAAGAAGAAGAAGCAAAGTTGTTCCCTCGTGTGATCCGTTTTCATAGTCTTACAGATTTTGATTTGAGTTCGAGTGTTCCTTCCAAAACAACAATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGTCGCTGCCCCCGCCGACTTCCTGAAGCTCCCCCTTCTTCACAAACCCCCCTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTCGCCCCAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCTTCCACCGGTTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACTCCTCCCCAAAGCCTCCTCCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTTAAATGCTCCGCCTGCCGCAACTGCTCTCACCATCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCATTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCTACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTATACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAATGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCTGCAGAGTTGACACCGGGATTCGATCTATGCGTGAATGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCACCGCCGAGGAACTATTTTCTGGAAACAGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGATGTGGGCTTCCATGAACGAACATCATCAACTTTTGGAACTTTGTTTCTTTATTTTTGTTAACTCGACTCACTGAGTTTGGAACTCGCAATCAATTAATTTTCGATTTGGTAAAATTATTATTATTATCATTATCATTATTATTATTATTATTAAAAAAGAGGTTTTTAAATTTTTAATAATAATAAGTTTGTACTTTGGATTGGATTCTCTTTTGTATACTACAAAAACTCATTGATTTTTGGACATTATGTTTTATTTGTATCACACCTTCCTTTTCTTTCCCCCCAACCTTACTTATATTTATATAAATCAATCCA

mRNA sequence

AATGAAAAATGAGATTGGATTGGGCCCAAAAAGAGAGCCCAACAGTTGAAAGAAGAAGAAGCAAAGTTGTTCCCTCGTGTGATCCGTTTTCATAGTCTTACAGATTTTGATTTGAGTTCGAGTGTTCCTTCCAAAACAACAATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGTCGCTGCCCCCGCCGACTTCCTGAAGCTCCCCCTTCTTCACAAACCCCCCTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTCGCCCCAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCTTCCACCGGTTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACTCCTCCCCAAAGCCTCCTCCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTTAAATGCTCCGCCTGCCGCAACTGCTCTCACCATCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCATTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCTACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTATACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAATGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCTGCAGAGTTGACACCGGGATTCGATCTATGCGTGAATGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCACCGCCGAGGAACTATTTTCTGGAAACAGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGATGTGGGCTTCCATGAACGAACATCATCAACTTTTGGAACTTTGTTTCTTTATTTTTGTTAACTCGACTCACTGAGTTTGGAACTCGCAATCAATTAATTTTCGATTTGGTAAAATTATTATTATTATCATTATCATTATTATTATTATTATTAAAAAAGAGGTTTTTAAATTTTTAATAATAATAAGTTTGTACTTTGGATTGGATTCTCTTTTGTATACTACAAAAACTCATTGATTTTTGGACATTATGTTTTATTTGTATCACACCTTCCTTTTCTTTCCCCCCAACCTTACTTATATTTATATAAATCAATCCA

Coding sequence (CDS)

ATGCCAGTCCTCTCCATTTCCCCATTCTTCCTTCTCATTCTTCTCTTCTCCTTCTTTCTCACACATCTCCCCAACCCCAATGCCACCGCCGTCGCTGCCCCCGCCGACTTCCTGAAGCTCCCCCTTCTTCACAAACCCCCCTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTCGCCCCAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCTTCCACCGGTTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACTCCTCCCCAAAGCCTCCTCCTCGTCGCCGATACCGGCAGCGACCTCGTCTGGGTTAAATGCTCCGCCTGCCGCAACTGCTCTCACCATCCTCCTTCCTCCGCCTTCCTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCATTGCTTCGACCCCCACTGCCGTCTCCTCCCCCACGCTCCTCACCATCTCTGTAACCACACGCGCCTCCACTCCCCTTGTCGCTTCCTCTACTCCTATGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGTCGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTCGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCGCCTACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTCTCACCAATGCCACAAAAATCAGCTATACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCCACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGCAATGGCGGCACGGTGGTGGATTCAGGGACAACGCTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCTGCAGAGTTGACACCGGGATTCGATCTATGCGTGAATGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCGCGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCACCGCCGAGGAACTATTTTCTGGAAACAGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGAAATGGGTTTTCGGTGATCGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGATGTGGGCTTCCATGA

Protein sequence

MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP*

Homology

[Close]

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.3e-58
Identity = 154/460 (33.48%), Postives = 233/460 (50.65%), Query Frame = 0


Query: 14  LLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS------ 73
           LL S F +   + +++++    D +     +K P    S  L  D+ R+  + +      
Sbjct: 55  LLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIP 114

Query: 74  ------RPNP-TLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 133
                  P P    S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR
Sbjct: 115 GRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCR 174

Query: 134 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 193
            C +      F PR S +++   C  PHCR L  A    CN  R    C +  SY DGS 
Sbjct: 175 RC-YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSF 234

Query: 194 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 253
           + G FS ET T +        +KG++ GCG    G       F GA G++GLG+G +SF 
Sbjct: 235 TVGDFSTETLTFR-----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFP 294

Query: 254 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 313
            Q G RF  KFSYCL+D + S  P+S +     +  +         +TPL  NP   TFY
Sbjct: 295 GQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFY 354

Query: 314 YITIHSITIDGVKLP-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK 373
           Y+ +  I++ G ++P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K
Sbjct: 355 YVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAK 414

Query: 374 LPNAAELTPGFDLCVNAS--GESRRPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCL 433
               A     FD C + S   E + P++  L FR   GA  + P  NY +  +  G  C 
Sbjct: 415 TLKRAPDFSLFDTCFDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCF 474

Query: 434 AIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
           A     +  G S+IGN+ QQGF + +D   SR+GF   GC
Sbjct: 475 AFAG--TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.9e-53
Identity = 127/374 (33.96%), Postives = 194/374 (51.87%), Query Frame = 0


Query: 84  GSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 143
           G G+Y +++ +GTP  S   + DTGSDL+W +C  C  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 144 CFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLK 203
           C   +C+ L   P   CN    ++ C++ Y Y DGS + G+ + ET T ++ S     + 
Sbjct: 152 CESQYCQDL---PSETCN----NNECQYTYGYGDGSTTQGYMATETFTFETSS-----VP 211

Query: 204 GLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPP 263
            ++FGCG    G      Q NGA G++G+G G +S  SQLG     +FSYC+  Y  S P
Sbjct: 212 NIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSP 271

Query: 264 PTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEI 323
            T  L +G     +P  + +    T L  + L+PT+YYIT+  IT+ G  L I  + +++
Sbjct: 272 ST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 331

Query: 324 DEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRP 383
            + G GG ++DSGTTLTYL + AY  V ++   ++ LP   E + G   C     +    
Sbjct: 332 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTV 391

Query: 384 SLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFD 443
            +P +  +  GG V     +N  +   EGV+CLA+ +  S  G S+ GN+ QQ   + +D
Sbjct: 392 QVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYD 436

Query: 444 KEESRLGFTRRGCG 458
            +   + F    CG
Sbjct: 452 LQNLAVSFVPTQCG 436

[Close]

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.7e-52
Identity = 138/410 (33.66%), Postives = 204/410 (49.76%), Query Frame = 0


Query: 53  QSLSSDTHRLSLLFSRPNPTLKSPLISGAST----GSGQYFVDIRLGTPPQSLLLVADTG 112
           Q L     R S    R    L  P  SG  T    G G+Y +++ +GTP Q    + DTG
Sbjct: 58  QLLERAIERGSRRLQRLEAMLNGP--SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTG 117

Query: 113 SDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSP 172
           SDL+W +C  C  C  +  +  F P+ SSSFS   C    C+        L + T  ++ 
Sbjct: 118 SDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLPCSSQLCQA-------LSSPTCSNNF 177

Query: 173 CRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARG 232
           C++ Y Y DGS + G    ET T  S+S     +  ++FGCG    G      Q NGA G
Sbjct: 178 CQYTYGYGDGSETQGSMGTETLTFGSVS-----IPNITFGCGENNQG----FGQGNGA-G 237

Query: 233 VMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYT 292
           ++G+GRG +S  SQL      KFSYC+     S P  S L++G   +S+    A   + T
Sbjct: 238 LVGMGRGPLSLPSQLD---VTKFSYCMTPIGSSTP--SNLLLGSLANSV---TAGSPNTT 297

Query: 293 PLQINPLSPTFYYITIHSITIDGVKLPINPAVWEID-EQGNGGTVVDSGTTLTYLTKTAY 352
            +Q + + PTFYYIT++ +++   +LPI+P+ + ++   G GG ++DSGTTLTY    AY
Sbjct: 298 LIQSSQI-PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAY 357

Query: 353 EEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFL 412
           + V +    ++ LP     + GFDLC     +     +P       GG +   P  NYF+
Sbjct: 358 QSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFI 417

Query: 413 ETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCG 458
               G++CLA+ +  S  G S+ GN+ QQ  L+ +D   S + F    CG
Sbjct: 418 SPSNGLICLAMGS--SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

[Close]

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.3e-51
Identity = 145/431 (33.64%), Postives = 207/431 (48.03%), Query Frame = 0


Query: 38  LKLPLLHK-----PPFSSPSQSLSSDTHRLSLLFSRPNPT--LKSPLISGASTGSGQYFV 97
           ++L L H+      P S     + +D  R SL+  + N T  +K  L SG   G+ QYF 
Sbjct: 49  VRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSGIDYGTAQYFT 108

Query: 98  DIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCR 157
           +IR+GTP +   +V DTGS+L WV C              F    S SF    C    C+
Sbjct: 109 EIRVGTPAKKFRVVVDTGSELTWVNCR--YRARGKDNRRVFRADESKSFKTVGCLTQTCK 168

Query: 158 LLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCG 217
           +       L       +PC + Y YADGS + G F+KET T+   +G    L G   GC 
Sbjct: 169 VDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGC- 228

Query: 218 FRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMI 277
                 S +G  F GA GV+GL     SF+S     +G KFSYCL+D+  +   +++L+ 
Sbjct: 229 ----SSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIF 288

Query: 278 GGGLHSLPLTNATKISY---TPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQG 337
           G        + +TK ++   TPL +  + P FY I +  I++    L I   VW  D   
Sbjct: 289 GS-------SRSTKTAFRRTTPLDLTRI-PPFYAINVIGISLGYDMLDIPSQVW--DATS 348

Query: 338 NGGTVVDSGTTLTYLTKTAYEEVLKSVRR-RVKLPNAAELTPGFDLCVNASGESRRPSLP 397
            GGT++DSGT+LT L   AY++V+  + R  V+L          + C + +       LP
Sbjct: 349 GGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLP 408

Query: 398 RLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGN-GFSVIGNLMQQGFLLEFDKE 457
           +L F L GGA F P  ++Y ++   GV CL    V +G    +VIGN+MQQ +L EFD  
Sbjct: 409 QLTFHLKGGARFEPHRKSYLVDAAPGVKCLGF--VSAGTPATNVIGNIMQQNYLWEFDLM 460

[Close]

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.8e-48
Identity = 125/384 (32.55%), Postives = 198/384 (51.56%), Query Frame = 0


Query: 75  SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPR 134
           S ++SG   GSG+YFV I +G+PP+   +V D+GSD+VWV+C  C+ C +      F P 
Sbjct: 118 SDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPA 177

Query: 135 HSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTLKS 194
            S S++   C    C  + ++  H          CR+   Y DGS     ++K T  L++
Sbjct: 178 KSGSYTGVSCGSSVCDRIENSGCH-------SGGCRYEVMYGDGS-----YTKGTLALET 237

Query: 195 LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYC 254
           L+ ++  ++ ++ GCG R  G       F GA G++G+G GS+SF  QL  + G  F YC
Sbjct: 238 LTFAKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYC 297

Query: 255 LMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKL 314
           L+  +     T  L+   G  +LP+      S+ PL  NP +P+FYY+ +  + + GV++
Sbjct: 298 LV--SRGTDSTGSLVF--GREALPV----GASWVPLVRNPRAPSFYYVGLKGLGVGGVRI 357

Query: 315 PINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVR-RRVKLPNAAELTPGFDLC 374
           P+   V+++ E G+GG V+D+GT +T L   AY       + +   LP A+ ++  FD C
Sbjct: 358 PLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTC 417

Query: 375 VNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEE-GVMCLAIRAVESGNGFSVIGN 434
            + SG      +P + F    G V   P RN+ +  ++ G  C A  A  S  G S+IGN
Sbjct: 418 YDLSG-FVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAA--SPTGLSIIGN 470

Query: 435 LMQQGFLLEFDKEESRLGFTRRGC 457
           + Q+G  + FD     +GF    C
Sbjct: 478 IQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 3.8e-268
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.2e-259
Identity = 446/459 (97.17%), Postives = 450/459 (98.04%), Query Frame = 0


Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNATAVAA ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.8e-226
Identity = 398/461 (86.33%), Postives = 419/461 (90.89%), Query Frame = 0


Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           +LS+S FF LILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSTFFHLILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADG 182
           CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RFR+G GAVFAPP RNYFLET EGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.2e-226
Identity = 395/461 (85.68%), Postives = 419/461 (90.89%), Query Frame = 0


Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           +LS+SPFF LILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSPFFHLILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADG 182
           CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLK+L+GSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL  LP+TNATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP +W IDEQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVF PP RNYFLETEEGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.2e-208
Identity = 363/458 (79.26%), Postives = 395/458 (86.24%), Query Frame = 0


Query: 6   ISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLL 65
           +  F LL+LL    L  L N      A P+ +LK PLLH  PFSSPSQ+LSSDTHRLSLL
Sbjct: 4   VPQFLLLLLLLLSSLADLSN------AIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLL 63

Query: 66  FS--RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCS 125
           FS  R +PTLKSPLISGASTGSGQYFV++ LGTPPQSLLLV DTGSDLVWVKCS CRNCS
Sbjct: 64  FSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCS 123

Query: 126 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSG 185
           HHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLYSYAD SLSSG
Sbjct: 124 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSG 183

Query: 186 FFSKETTTLKSLSG--SEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSS 245
           FFSK+ TT  + SG  ++  L  LSFGCGFRISGPSVSGA+F GARGVMGLGRG ISFSS
Sbjct: 184 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSS 243

Query: 246 QLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYY 305
           QLG RFGN FSYCLMDYTLSPPPTS+LMIGGGL SLP+TNA+KISYTPLQINPLSPTFYY
Sbjct: 244 QLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 303

Query: 306 ITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLP 365
           I + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP
Sbjct: 304 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 363

Query: 366 NAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAV 425
            A +L+PGFDLCVNAS ESR  SLP++RFR+GGG VFAPP RNYF+ETEEGVMCLAIR V
Sbjct: 364 RALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 423

Query: 426 ESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           +SGNGFSVIGNLMQQGFLLEFD+E+SR+GF+RRGCGLP
Sbjct: 424 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

[Close]

BLAST of CSPI05G08550 vs. NCBI nr
Match: XP_004143702.1 (aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical protein Csa_000435 [Cucumis sativus])

HSP 1 Score: 933.3 bits (2411), Expect = 7.9e-268
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. NCBI nr
Match: XP_008467208.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo])

HSP 1 Score: 903.3 bits (2333), Expect = 8.7e-259
Identity = 446/459 (97.17%), Postives = 450/459 (98.04%), Query Frame = 0


Query: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNATAVAA ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 411/457 (89.93%), Postives = 431/457 (94.31%), Query Frame = 0


Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           +L ISPFF  I LF FFLT   N NATA    AD+LKLPLLHKPPFSSPSQ+LSSDTHRL
Sbjct: 1   MLPISPFFHFIFLF-FFLT---NFNATA----ADYLKLPLLHKPPFSSPSQALSSDTHRL 60

Query: 63  SLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNC 122
           SLLFSRPNPTLKSPLISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 61  SLLFSRPNPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120

Query: 123 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSS 182
           SHHPPS+AFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTR HSPCRFLYSYADGSLSS
Sbjct: 121 SHHPPSTAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRFHSPCRFLYSYADGSLSS 180

Query: 183 GFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQ 242
           GFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQF+GARGVMGLGRGSISFSSQ
Sbjct: 181 GFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGARGVMGLGRGSISFSSQ 240

Query: 243 LGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYI 302
           LGRRFGNKFSYCLMDYTLSPPPTS+LMIGGG  SLP+TNATKISYTPLQINPLSPTFYYI
Sbjct: 241 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISYTPLQINPLSPTFYYI 300

Query: 303 TIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPN 362
            IHSIT+DGVKLPINPAVW +D+QGNGGTVVDSGTTLTYL K AY+EVLK+VRRRVKLP+
Sbjct: 301 AIHSITVDGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAYDEVLKAVRRRVKLPS 360

Query: 363 AAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVE 422
           A+ELTPGFDLCVNAS  SRRPSLPRLRFRLGGGAVFAPPPRNYFLETEE VMCLAIR V+
Sbjct: 361 ASELTPGFDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVMCLAIRPVD 420

Query: 423 SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           SGNGFSVIGNLMQQGFLLEFDK+ +RLGF+RRGCGLP
Sbjct: 421 SGNGFSVIGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

[Close]

BLAST of CSPI05G08550 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797.7 bits (2059), Expect = 5.2e-227
Identity = 398/461 (86.33%), Postives = 420/461 (91.11%), Query Frame = 0


Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           +LS+SPFF  ILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSPFFHFILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADG 182
           CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVFAPP RNYFLETEEGVMCL+I
Sbjct: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 794.3 bits (2050), Expect = 5.7e-226
Identity = 398/461 (86.33%), Postives = 419/461 (90.89%), Query Frame = 0


Query: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           +LS+S FF LILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSTFFHLILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADG 182
           CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RFR+G GAVFAPP RNYFLET EGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CSPI05G08550 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 596.7 bits (1537), Expect = 1.6e-170
Identity = 296/456 (64.91%), Postives = 355/456 (77.85%), Query Frame = 0


Query: 10  FLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSR- 69
           F L    S FL  LP  N  AV+    +LKLPLL K PF SP+Q+L+ DT RL  L  R 
Sbjct: 6   FFLCSFLSLFL--LPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 70  -PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 129
            P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 130 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 189
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGSL+SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 190 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 249
           F++ETT+LK+ SG E  LK ++FGCGFRISG SVSG  FNGA GVMGLGRG ISF+SQLG
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 245

Query: 250 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 309
           RRFGNKFSYCLMDYTLSPPPTS+L+IG G   +     +K+ +TPL  NPLSPTFYY+ +
Sbjct: 246 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI-----SKLFFTPLLTNPLSPTFYYVKL 305

Query: 310 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 369
            S+ ++G KL I+P++WEID+ GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP A 
Sbjct: 306 KSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 365

Query: 370 ELTPGFDLCVNASGESR-RPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVES 429
            LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++V+ 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 425

Query: 430 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
             GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

[Close]

BLAST of CSPI05G08550 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-113
Identity = 227/456 (49.78%), Postives = 270/456 (59.21%), Query Frame = 0


Query: 10  FLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSR- 69
           F L    S FL  LP  N  AV+    +LKLPLL K PF SP+Q+L+ DT RL  L  R 
Sbjct: 6   FFLCSFLSLFL--LPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 70  -PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 129
            P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 130 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPHHLCNHTRLHSPCRFLYSYADGSLSSGF 189
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGSL+SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 190 FSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 249
           F++ETT+LK+ SG E  LK ++FGCGFRISG SVS                         
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS------------------------- 245

Query: 250 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 309
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 310 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 369
                                 GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP A 
Sbjct: 306 ----------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 350

Query: 370 ELTPGFDLCVNASGESR-RPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVES 429
            LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++V+ 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 350

Query: 430 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
             GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

[Close]

BLAST of CSPI05G08550 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 232.3 bits (591), Expect = 8.1e-61
Identity = 148/391 (37.85%), Postives = 204/391 (52.17%), Query Frame = 0


Query: 73  LKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 132
           L + L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C  H     + 
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYD 204

Query: 133 PRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSKETTTL 192
           P+ S+SF    C DP C L+  +P         +  C + Y Y D S ++G F+ ET T+
Sbjct: 205 PKTSASFKNITCNDPRCSLI-SSPDPPVQCESDNQSCPYFYWYGDRSNTTGDFAVETFTV 264

Query: 193 KSLS----GSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFG 252
              +     SE  +  + FGCG    G       F+GA G++GLGRG +SFSSQL   +G
Sbjct: 265 NLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLYG 324

Query: 253 NKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSIT 312
           + FSYCL+D   +   +S L+ G     L  TN    S+   + N +  TFYYI I SI 
Sbjct: 325 HSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKSIL 384

Query: 313 IDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK--LPNAAEL 372
           + G  L I    W I   G+GGT++DSGTTL+Y  + AYE +      ++K   P   + 
Sbjct: 385 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF 444

Query: 373 TPGFDLCVNASG-ESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGN 432
            P  D C N SG E     LP L      G V+  P  N F+   E ++CLAI       
Sbjct: 445 -PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST 504

Query: 433 GFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
            FS+IGN  QQ F + +D + SRLGFT   C
Sbjct: 505 -FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of CSPI05G08550 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 228.8 bits (582), Expect = 8.9e-60
Identity = 154/460 (33.48%), Postives = 233/460 (50.65%), Query Frame = 0


Query: 14  LLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS------ 73
           LL S F +   + +++++    D +     +K P    S  L  D+ R+  + +      
Sbjct: 55  LLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIP 114

Query: 74  ------RPNP-TLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 133
                  P P    S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR
Sbjct: 115 GRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCR 174

Query: 134 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 193
            C +      F PR S +++   C  PHCR L  A    CN  R    C +  SY DGS 
Sbjct: 175 RC-YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSF 234

Query: 194 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 253
           + G FS ET T +        +KG++ GCG    G       F GA G++GLG+G +SF 
Sbjct: 235 TVGDFSTETLTFR-----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFP 294

Query: 254 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 313
            Q G RF  KFSYCL+D + S  P+S +     +  +         +TPL  NP   TFY
Sbjct: 295 GQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFY 354

Query: 314 YITIHSITIDGVKLP-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK 373
           Y+ +  I++ G ++P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K
Sbjct: 355 YVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAK 414

Query: 374 LPNAAELTPGFDLCVNAS--GESRRPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCL 433
               A     FD C + S   E + P++  L FR   GA  + P  NY +  +  G  C 
Sbjct: 415 TLKRAPDFSLFDTCFDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCF 474

Query: 434 AIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
           A     +  G S+IGN+ QQGF + +D   SR+GF   GC
Sbjct: 475 AFAG--TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of CSPI05G08550 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 222.6 bits (566), Expect = 6.4e-58
Identity = 154/454 (33.92%), Postives = 232/454 (51.10%), Query Frame = 0


Query: 17  SFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFSRPNPTLKSP 76
           S  L+H+   ++ + A+PAD   L L      S   +S++S    L+ + +  N T ++P
Sbjct: 62  SVHLSHVDALSSFSDASPADLFNLRLQRD---SLRVKSITS----LAAVSTGRNATKRTP 121

Query: 77  ---------LISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 136
                    +ISG S GSG+YF+ + +GTP  ++ +V DTGSD+VW++CS C+ C ++  
Sbjct: 122 RTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKAC-YNQT 181

Query: 137 SSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSK 196
            + F P+ S +F+   C    CR L  +   +   TR    C +  SY DGS + G FS 
Sbjct: 182 DAIFDPKKSKTFATVPCGSRLCRRLDDSSECV---TRRSKTCLYQVSYGDGSFTEGDFST 241

Query: 197 ETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRF 256
           ET T        + L     GCG    G       F GA G++GLGRG +SF SQ   R+
Sbjct: 242 ETLTFHGARVDHVPL-----GCGHDNEG------LFVGAAGLLGLGRGGLSFPSQTKNRY 301

Query: 257 GNKFSYCLMDYT----LSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYIT 316
             KFSYCL+D T     S PP++ +    G  ++P T+     +TPL  NP   TFYY+ 
Sbjct: 302 NGKFSYCLVDRTSSGSSSKPPSTIVF---GNAAVPKTSV----FTPLLTNPKLDTFYYLQ 361

Query: 317 IHSITIDGVKLP-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPN 376
           +  I++ G ++P ++ + +++D  GNGG ++DSGT++T LT+ AY  +  + R       
Sbjct: 362 LLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLK 421

Query: 377 AAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVE 436
            A     FD C + SG +    +P + F  GGG V  P          EG  C A     
Sbjct: 422 RAPSYSLFDTCFDLSGMT-TVKVPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAG-- 481

Query: 437 SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
           +    S+IGN+ QQGF + +D   SR+GF  R C
Sbjct: 482 TMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of CSPI05G08550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNJ3	1.3e-58	33.48	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	7.9e-53	33.96	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	8.7e-52	33.66	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LTW4	4.3e-51	33.64	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q9LHE3	5.8e-48	32.55	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of CSPI05G08550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNH6	3.8e-268	100.00	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465...	[more]
A0A1S3CSZ8	4.2e-259	97.17	aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ...	[more]
A0A6J1HXS2	2.8e-226	86.33	aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P...	[more]
A0A6J1ELQ4	6.2e-226	85.68	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1EPZ3	1.2e-208	79.26	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CSPI05G08550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143702.1	7.9e-268	100.00	aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical pr...	[more]
XP_008467208.1	8.7e-259	97.17	PREDICTED: aspartyl protease family protein 2 [Cucumis melo]	[more]
XP_038907006.1	1.2e-236	89.93	aspartyl protease family protein 2 [Benincasa hispida]	[more]
XP_023549997.1	5.2e-227	86.33	aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022969957.1	5.7e-226	86.33	aspartyl protease family protein 2 [Cucurbita maxima]	[more]

BLAST of CSPI05G08550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25700.1	1.6e-170	64.91	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.2	1.1e-113	49.78	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	8.1e-61	37.85	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	8.9e-60	33.48	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	6.4e-58	33.92	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 94..114 score: 49.53 coord: 428..443 score: 18.99 coord: 331..342 score: 41.24
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 88..272 e-value: 2.2E-48 score: 164.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 67..271 e-value: 1.1E-45 score: 158.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 277..457 e-value: 3.2E-50 score: 172.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 82..456
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 299..452 e-value: 1.7E-36 score: 125.4
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 12..457
None	No IPR available	PANTHER	PTHR47967:SF72	BNAA02G27600D PROTEIN	coord: 12..457
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 88..452 score: 40.986813
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 87..456 e-value: 1.40021E-84 score: 258.732

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G08550.1	CSPI05G08550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity