CSPI05G08270 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G08270
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein BOLA4, chloroplastic/mitochondrial
Location	Chr5: 7022881 .. 7027385 (-)
RNA-Seq Expression	CSPI05G08270
Synteny	CSPI05G08270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTTTTTCTTTTTTTCATTATTTTGTTAACATTGGAACAAATTTCACTATGTGGAACTTTTGGCAGACTCGGACCATACATCGACACGGGAAAGCAAACAAGAAAGATAGAATAAAACCGATCAAGTAGAGCAGTTCCATCGATTCAGCTGCGCCTAAGACTTTGATCATGATGATTAAGGTTCCTCAAGTTGCGTTCCGTCGGGTTCTTCCAATATTCCTTCCCCGCAACCGACCTTTTTCGGTTGTCTCCCGCCAAGTCACTTCCAATTGTGTGTGCACCCCCGCCGTTGTCGCTGTAATTATCTCAACATCCACCACCGGCCTCCGTAGTAAGAGCAGTAATGTTGCGAAGTATAGCCGTGCCAATGGAACATTTCCAAAATGGGGTTCGAGTCGGAACTTGAGCATTAGAGCCACTCAAGTGAATGAATCTGGATCTATTGATTCCCCGCTCATGCAGTCAATGGAGAACAAGGTGCTTTTTGTTTAATCTATGGTTTCTTTATCGTTTTAGGTTTATAGTTTTCTACGTTTTCGTTTATTTGAGCAAATACGTGCGTTGCTTTTTTGGGGGAAATTAGGGCAAGTTAATGAGCTAATTTAAGAGGAATGAAATCTCCCAGTTGATTTTAGTTTAGAAACGTTCTTAGTAGCTCGGGATAAAGACCTTAAAGCCGATTTTATGAGATGATTCTTTCTTATCTTATGTTTTCGTTTTAACATGTCTGCTATCCAACTTATTTTCAGTTTCTTTTCTGCAAATGTGTATTTGTTTGTAAAACGGATGTAGCTTTGTTTTTCTCGCTCCAATATATTATCCACCTAGTTGTTTAGTTCTTACCGGTGCCATGTTATTAGAAGGGCCACGACCACAGCTGGTTGACTAATTCATCCCGAGGTCGCCTGTGCGTAGTGGCATACGCAGAAACTGATGGCATACTTGATAAGAAAATCAATTCATTTCTCCAAAGAAAAGGATTTTCCCTTCAATGAAATACAGATAGATCTGCACACTTAATCGCTACTAGGAAGAGACCAGCACCTGCCATGAGTAGAAAATCCAGCACTTGAGTGCCACTGACATCTGCCAAGCCAATAGCAGGTGGTGGGTGTGAAGGTTTTGATAGAATGCATTCCAATCTCACCGTTTTTGGTTCTTGTACCAGCTTGAGATGGTGGTGGACAACCGAAACAACTGGCAGCGCTGAGGTGGTGGTATAAGTAGTGGTATTATTGGATGATGATTATGAGAAGAAATCCAGTAAAAAAGGAAAATTAAAAAAATCAAGGAAAGCACAAAAAAGAAGGAGAAATAAAGAAATCTTTTAAAAGATTGAAGCTTTTTGTCATAGGTGGAAGAAAGATCTCATCTTTTAGAGTTTCTAAGAGAACCCACTTTGTATTTTTTACCCCCTCAATAGGAGGTAGGTAGAAAATGAAGTAGTGATTCTAAATGAAGATCAAAGAAAGAGAAGAAATAAAGAGTGGGTTTAATTTGCATGCCGTGAAGAGATCAAGAACAATGTGAAGGAGCATATCATAAATTCGACGTCGTTGATAATATTGGAAACAACCATTTTTTAGAAAGACCAAAAAGAGAGTATTTTTTTCTTTTTTGTGTTTCTTTAGAAGAAGAGAAATGGAAGAAGGAAGAAGAGCAGAAAAAAAGGTCATGAGTTTATGGTGTATGAAGAAGGGAAATGGGGAAATTATCAGAGATCATAGGATTTTCTTTTCAAGGGGAATTATCGTGATGATTGTCCTTGGTTGATTATGTGATTTTGACTTGGGAGTCACGAGCTCTTTGTCAATTCCAACAAAAGAAAAACTTTCTTTCAATGTCCATTGTTGTGCGTGGCTATGGGGAAGTCATGTTGGGAGAATGAAGTAGTTACTGTCAACAGAATCATGGTTGGCAGATAGCTCCGGTGAGAAGGGGGAGAGAGGTGGAAAAGAGAAGAGAGAACATTGTTTTCAATTCATCATTTATGGTTAGAAAACAAAAGCTGTGCAATCCTTGTCTTGGATATTTATTGAAATAAGTCTGGTAAAGTATATTTGAATGGGAATTGTTATTTCTTTCTAGAGTCGTTGTATCTATTTCAAGATGATTAAATTTTTGTTTATCGTGTTGACTTTGCAAATTTCTTCATCCAGATTAAGGAACAACTAGATGCACAATCCGTCTCAGTAAAAGATGCTTATGGGGATGGACGACATGTGAGGTGAGGGCTTTTCTCTATAATTCCTTTTTATGGTTGCTTAGTTTAAGAAGCTTGATTTCTCCAGAATTTAAATTGGAACACATTTATTTTTTAGGGAATTGGTCTTATGTTTTGTTAAGCTGTATTATTATTTTTTATTTGTGTCATGTTATTTTGAGCATTAGCCTCTCCTCTCTTCCCCCCCCCCCCTTTTCTACTCCCGCCGCCGTAGCAACCACCATTTCCTTCTATGAAGCCCCTTCTTCTTTGAGATTCCCACATTTTAAATCTTTAACTTCAAACCCCTCCCCCCCCCCCCCCCCCCCCCCCCCATTTTTTAAAAATTGAGAGCTCCAAGAGGGATGTTTGAAAAAGTCGTATCCATATACATCCTCTTAAGAACTACATCTCCCGAGGAACACTTTTTTGTTCCTATCATAAAAAGTTGGGGACCCGTTTAATTGTCTTGCGGGGTAAATATGTACATATTTTGTCTGTTAAATGTATATAGTATCTAGCAAAAAGAAATTTGGTTTCCACTTTGTATGGAAGAGAAATTGATAGCAACCAAAACACAATCAAATTATTTAAATGACAGTAAATGACTTAAACTTACAAGATAAGCAAATAGTTTGAGGTACTTGAACCCTCCTCTCTTGAGACCTCTCTAATATCGTAATTGACTCACCAAGATAATACCAATTTTCCCTGTTCTCCATCCTTCTATTTATAACCAAATTCCATAACTAACTCCTATAGCTAATTACTAATGTACCCTTAAGATTCCTCTTACACCCTTATACCATTCCTATTGGAGATGGATAGGCTTATCTTCAACTACCCTTTTGGCATCTGTTTCTTGCCAAAGCGCAGTTTATGTCCCATGGGTTTGCTATGAAGTCTTCTCATTCTTGTAAACGATTGATGTCTCCAAGCTCAACTAGTTCAAATTTATTTTGCAATCAAAAGGTGAGAGAATTGAATCTCTACCTCCGTATATTGTTGAATTAAAAATATTGATACTGAACCTCCAACGTAATTCCCTAATAAATTGATTAAATGTCTTTATCTCTGTTTTTCCTACTTGGCAAAGTTCGTTGGAGATGCATGTTGATTGGGGAGTTTCCTGTAGTTTGCATGTTACGTTGAAGTATAATTGGCTTCATTGGCCATATCCAAAGGACAAGTCAAGATGAATTTAGTACTCAGTTAAGTTAATTTTTGTTGCAGCATTGATGTTGTTTCTTCAGCCTTTGAGGGACAGACGGCTGTGAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAAAGCACAGTACATGCAGTTGATCAAATGACCACCAGAACACCTGCGGAAGCAGCAGCTCAGAATTCATAACTCTCAAAGTTTGTCCAAACCTTCTTGTCTTTGCCAATAAATTTCTTGCAAAAAAGTGTGCTCATATTATTTCTTTTCACCTGTTGATTAATGGTAAAAGTTTTTATCTTTGATCTTACATTCATAACTGATGGTCAAGGAATCAGCTTGCCCTTTTTTGCATGGGTTCCACCCCCTCCCCTTTTTGGAACACCCAACATCTGGCTTGAGAACTTAAAATGTCATTCTTAACCAAATCATATTACCAAACCCTCTGTTCTAGAGCTCTAAATATTCTAAATTTAAACCTTTTTTCCCCGTTGCATGCACTAGTCTATTTCCTGTTCTTTTTCTCATGGACGTACGTGACTCAGTCACTTTCAAGCAAAATAAGACCTAACAAATAATTACAAAAAAAAAAAAAAGAAATTTTTTTTGCCATCGAGGTCCAAAGAGAAATAGATCCTAACAAGGGTCCCAACACTAGGATCACAGTCCCTGTCCATTTCGATTCTATTGTCTCTATTTTCCTTAAAACCCCCTTTGACTTGCCACCAGATCCTCATATCCTAAAACAACAGATGTAGAAATATTTCCTTGATCATTTCAGTTTAATCTCTGTGTCGGGCTACCTAAAAAACCAAATACCTCAAAGAAGTAACTCCGAACAGGCCAGACAAGTTATAGCTCCAAAGGATATTATCCAAGTCTTCCTCGAGTCTCCAACAAAGGAAGCAGCAAAATGTCCCAACCAAATAAGTCAACCTTCTTGAAAGACAATCAAGAATGTTAACTCTTCTATGAATAACCTGGCAAATGGAGAACTCTATCTCTGAAATTTTCACCTTCCATAGGAAAGAAAAAAGTAACCCCCTTTGTCACCTTCCCATCTCATGTAAAAATAAATTAAGTTTATGAACCTCACTACCCC

mRNA sequence

TTTCTTTTTCTTTTTTTCATTATTTTGTTAACATTGGAACAAATTTCACTATGTGGAACTTTTGGCAGACTCGGACCATACATCGACACGGGAAAGCAAACAAGAAAGATAGAATAAAACCGATCAAGTAGAGCAGTTCCATCGATTCAGCTGCGCCTAAGACTTTGATCATGATGATTAAGGTTCCTCAAGTTGCGTTCCGTCGGGTTCTTCCAATATTCCTTCCCCGCAACCGACCTTTTTCGGTTGTCTCCCGCCAAGTCACTTCCAATTGTGTGTGCACCCCCGCCGTTGTCGCTGTAATTATCTCAACATCCACCACCGGCCTCCGTAGTAAGAGCAGTAATGTTGCGAAGTATAGCCGTGCCAATGGAACATTTCCAAAATGGGGTTCGAGTCGGAACTTGAGCATTAGAGCCACTCAAGTGAATGAATCTGGATCTATTGATTCCCCGCTCATGCAGTCAATGGAGAACAAGATTAAGGAACAACTAGATGCACAATCCGTCTCAGTAAAAGATGCTTATGGGGATGGACGACATGTGAGCATTGATGTTGTTTCTTCAGCCTTTGAGGGACAGACGGCTGTGAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAAAGCACAGTACATGCAGTTGATCAAATGACCACCAGAACACCTGCGGAAGCAGCAGCTCAGAATTCATAACTCTCAAAGTTTGTCCAAACCTTCTTGTCTTTGCCAATAAATTTCTTGCAAAAAAGTGTGCTCATATTATTTCTTTTCACCTGTTGATTAATGGTAAAAGTTTTTATCTTTGATCTTACATTCATAACTGATGGTCAAGGAATCAGCTTGCCCTTTTTTGCATGGGTTCCACCCCCTCCCCTTTTTGGAACACCCAACATCTGGCTTGAGAACTTAAAATGTCATTCTTAACCAAATCATATTACCAAACCCTCTGTTCTAGAGCTCTAAATATTCTAAATTTAAACCTTTTTTCCCCGTTGCATGCACTAGTCTATTTCCTGTTCTTTTTCTCATGGACGTACGTGACTCAGTCACTTTCAAGCAAAATAAGACCTAACAAATAATTACAAAAAAAAAAAAAAGAAATTTTTTTTGCCATCGAGGTCCAAAGAGAAATAGATCCTAACAAGGGTCCCAACACTAGGATCACAGTCCCTGTCCATTTCGATTCTATTGTCTCTATTTTCCTTAAAACCCCCTTTGACTTGCCACCAGATCCTCATATCCTAAAACAACAGATGTAGAAATATTTCCTTGATCATTTCAGTTTAATCTCTGTGTCGGGCTACCTAAAAAACCAAATACCTCAAAGAAGTAACTCCGAACAGGCCAGACAAGTTATAGCTCCAAAGGATATTATCCAAGTCTTCCTCGAGTCTCCAACAAAGGAAGCAGCAAAATGTCCCAACCAAATAAGTCAACCTTCTTGAAAGACAATCAAGAATGTTAACTCTTCTATGAATAACCTGGCAAATGGAGAACTCTATCTCTGAAATTTTCACCTTCCATAGGAAAGAAAAAAGTAACCCCCTTTGTCACCTTCCCATCTCATGTAAAAATAAATTAAGTTTATGAACCTCACTACCCC

Coding sequence (CDS)

ATGATGATTAAGGTTCCTCAAGTTGCGTTCCGTCGGGTTCTTCCAATATTCCTTCCCCGCAACCGACCTTTTTCGGTTGTCTCCCGCCAAGTCACTTCCAATTGTGTGTGCACCCCCGCCGTTGTCGCTGTAATTATCTCAACATCCACCACCGGCCTCCGTAGTAAGAGCAGTAATGTTGCGAAGTATAGCCGTGCCAATGGAACATTTCCAAAATGGGGTTCGAGTCGGAACTTGAGCATTAGAGCCACTCAAGTGAATGAATCTGGATCTATTGATTCCCCGCTCATGCAGTCAATGGAGAACAAGATTAAGGAACAACTAGATGCACAATCCGTCTCAGTAAAAGATGCTTATGGGGATGGACGACATGTGAGCATTGATGTTGTTTCTTCAGCCTTTGAGGGACAGACGGCTGTGAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAAAGCACAGTACATGCAGTTGATCAAATGACCACCAGAACACCTGCGGAAGCAGCAGCTCAGAATTCATAA

Protein sequence

MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVAKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGDGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS*

Homology

[Close]

BLAST of CSPI05G08270 vs. ExPASy Swiss-Prot
Match: Q9LF68 (Protein BOLA4, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BOLA4 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.7e-32
Identity = 86/158 (54.43%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 16  IFLPRNRPFSVVSRQVT--SNCVCTPAVVAVIISTSTTGLRSKSSNVAKYSRANGTFPKW 75
           +F    RPF+  S  +   +N  C    V+ ++S   T   S   N  + SR +G     
Sbjct: 25  LFQSSKRPFASFSAPLVRFTNSRC----VSAVLSRKETAPSSIYGN--RVSRVSGFGALD 84

Query: 76  GSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGDGRHVSIDVVSSA 135
             S N S +++Q+N++GSID  LMQSME KIKEQL+A+SV+V D  GDGRHV I+VVSSA
Sbjct: 85  IRSLNFSTKSSQINDAGSIDQTLMQSMELKIKEQLNAESVTVTDMSGDGRHVCINVVSSA 144

Query: 136 FEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAE 172
           FEGQ+AVNRQRMVYKAIWEELQ+ VHAVDQMTT+TP+E
Sbjct: 145 FEGQSAVNRQRMVYKAIWEELQNVVHAVDQMTTKTPSE 176

[Close]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL
Match: A0A0A0KRR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G172860 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.0e-88
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60
           MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV
Sbjct: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60

Query: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120
           AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG
Sbjct: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120

Query: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 177

[Close]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL
Match: A0A1S3CT29 (protein BOLA4, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103504639 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.9e-82
Identity = 166/176 (94.32%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           MIKVPQ+AFRRVLP+ LPRN+PFSVVSRQ+TSNCVCT AVVAVI S S TGLRSKSSNV 
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL
Match: A0A5A7UJH5 (Protein BOLA4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00900 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.4e-82
Identity = 165/176 (93.75%), Postives = 169/176 (96.02%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           MIKVPQ+AFRRVLP+ LPRN+PFSVVSRQ+TSNCVCT AVVAVI S S TGLRSKSSNV 
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KYSRANGTFPKWGSSRN SIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNSSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL
Match: A0A6J1DZG6 (protein BOLA4, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111026033 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.7e-70
Identity = 146/176 (82.95%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           M++VPQVAFRRVLP+F+ R+ PF VVSRQ+T+NCV T A  A I S +TT +R KSSNVA
Sbjct: 3   MVRVPQVAFRRVLPLFVHRDVPFLVVSRQITTNCVRTAA--AAISSRTTTSVRCKSSNVA 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KY  ANGTF KWGSSRNLSIRATQVN+SGSIDSPLMQSMENKIKEQLDAQSV+VKDAYGD
Sbjct: 63  KYGSANGTFSKWGSSRNLSIRATQVNDSGSIDSPLMQSMENKIKEQLDAQSVTVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDV+SSAFEGQ+AVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAA +NS
Sbjct: 123 GRHVSIDVISSAFEGQSAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAVKNS 176

[Close]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL
Match: A0A6J1K139 (protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111491454 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.0e-68
Identity = 142/176 (80.68%), Postives = 155/176 (88.07%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           MIKVPQVAFRRVLP+F+ RN  F +VS+Q T+NCV T AV A I +T+ TG+ SKSSNV 
Sbjct: 6   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTNCVRTAAVSAAITTTTATGVCSKSSNVL 65

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           K+S  NG F  WGSSRN+ +RATQVN+SGSIDSPLMQSMENKIKEQL+AQSVSVKDAYGD
Sbjct: 66  KFSYVNGRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 125

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTP EA AQNS
Sbjct: 126 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPTEAEAQNS 181

[Close]

BLAST of CSPI05G08270 vs. NCBI nr
Match: XP_004143637.1 (protein BOLA4, chloroplastic/mitochondrial [Cucumis sativus] >KGN50406.1 hypothetical protein Csa_000569 [Cucumis sativus])

HSP 1 Score: 334.0 bits (855), Expect = 8.2e-88
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60
           MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV
Sbjct: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60

Query: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120
           AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG
Sbjct: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120

Query: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 177

[Close]

BLAST of CSPI05G08270 vs. NCBI nr
Match: XP_008467240.1 (PREDICTED: protein BOLA4, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 315.1 bits (806), Expect = 3.9e-82
Identity = 166/176 (94.32%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           MIKVPQ+AFRRVLP+ LPRN+PFSVVSRQ+TSNCVCT AVVAVI S S TGLRSKSSNV 
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of CSPI05G08270 vs. NCBI nr
Match: KAA0055158.1 (protein BOLA4 [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 1.9e-81
Identity = 165/176 (93.75%), Postives = 169/176 (96.02%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           MIKVPQ+AFRRVLP+ LPRN+PFSVVSRQ+TSNCVCT AVVAVI S S TGLRSKSSNV 
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KYSRANGTFPKWGSSRN SIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNSSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of CSPI05G08270 vs. NCBI nr
Match: XP_038906458.1 (protein BOLA4, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 303.1 bits (775), Expect = 1.5e-78
Identity = 158/177 (89.27%), Postives = 169/177 (95.48%), Query Frame = 0


Query: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60
           MMIKVP VAFRRVLP+F+  N+PFSVVSRQ+TSNCVC  AVVA I STSTTG+RSKSSN+
Sbjct: 2   MMIKVPHVAFRRVLPLFVHHNQPFSVVSRQITSNCVCNAAVVAAITSTSTTGVRSKSSNL 61

Query: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120
           A+YSR+NGTFPKWGSSR+LSIRATQVNESGSIDSPLM+SMENKIK+QLDAQSVSVKDAYG
Sbjct: 62  ARYSRSNGTFPKWGSSRSLSIRATQVNESGSIDSPLMKSMENKIKDQLDAQSVSVKDAYG 121

Query: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAA NS
Sbjct: 122 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAHNS 178

[Close]

BLAST of CSPI05G08270 vs. NCBI nr
Match: XP_022159690.1 (protein BOLA4, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 274.2 bits (700), Expect = 7.7e-70
Identity = 146/176 (82.95%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNVA 61
           M++VPQVAFRRVLP+F+ R+ PF VVSRQ+T+NCV T A  A I S +TT +R KSSNVA
Sbjct: 3   MVRVPQVAFRRVLPLFVHRDVPFLVVSRQITTNCVRTAA--AAISSRTTTSVRCKSSNVA 62

Query: 62  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 121
           KY  ANGTF KWGSSRNLSIRATQVN+SGSIDSPLMQSMENKIKEQLDAQSV+VKDAYGD
Sbjct: 63  KYGSANGTFSKWGSSRNLSIRATQVNDSGSIDSPLMQSMENKIKEQLDAQSVTVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178
           GRHVSIDV+SSAFEGQ+AVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAA +NS
Sbjct: 123 GRHVSIDVISSAFEGQSAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAVKNS 176

[Close]

BLAST of CSPI05G08270 vs. TAIR 10
Match: AT5G17560.1 (BolA-like family protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-33
Identity = 86/158 (54.43%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 16  IFLPRNRPFSVVSRQVT--SNCVCTPAVVAVIISTSTTGLRSKSSNVAKYSRANGTFPKW 75
           +F    RPF+  S  +   +N  C    V+ ++S   T   S   N  + SR +G     
Sbjct: 25  LFQSSKRPFASFSAPLVRFTNSRC----VSAVLSRKETAPSSIYGN--RVSRVSGFGALD 84

Query: 76  GSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGDGRHVSIDVVSSA 135
             S N S +++Q+N++GSID  LMQSME KIKEQL+A+SV+V D  GDGRHV I+VVSSA
Sbjct: 85  IRSLNFSTKSSQINDAGSIDQTLMQSMELKIKEQLNAESVTVTDMSGDGRHVCINVVSSA 144

Query: 136 FEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAE 172
           FEGQ+AVNRQRMVYKAIWEELQ+ VHAVDQMTT+TP+E
Sbjct: 145 FEGQSAVNRQRMVYKAIWEELQNVVHAVDQMTTKTPSE 176

[Close]

BLAST of CSPI05G08270 vs. TAIR 10
Match: AT4G26500.1 (chloroplast sulfur E )

HSP 1 Score: 43.5 bits (101), Expect = 2.1e-04
Identity = 27/87 (31.03%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 100 MENKIKEQLDAQSVSVKD--------------AYGDGR-HVSIDVVSSAFEGQTAVNRQR 159
           +  K++++LD   + V+D              A  DG  H ++ +VS AF+G++ V R R
Sbjct: 285 IREKLEKELDPVELEVEDVSYQHAGHAAVRGSAGDDGETHFNLRIVSDAFQGKSLVKRHR 344

Query: 160 MVYKAIWEELQSTVHAVDQMTTRTPAE 172
           ++Y  + +EL+S +HA+  +  +TPAE
Sbjct: 345 LIYDLLQDELKSGLHAL-SIVAKTPAE 370

[Close]

BLAST of CSPI05G08270 vs. TAIR 10
Match: AT1G55805.1 (BolA-like family protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.7e-04
Identity = 35/123 (28.46%), Postives = 63/123 (51.22%), Query Frame = 0


Query: 55  SKSSNVAKYSRANGTFPKWGS-SRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSV 114
           SKS+     S A  +  K GS S  +  RA+++ E    +   ++ +   +  Q  A   
Sbjct: 40  SKSTGTGSRSVAMSSVEKTGSDSGAIENRASRMREKLQKELEPVELVIEDVSYQ-HAGHA 99

Query: 115 SVKDAYGDGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAA 174
            +K    D  H ++ +VS  FEG   V R R+VY  + EEL + +HA+  + ++TP+E+ 
Sbjct: 100 GMKGRTDDETHFNVKIVSKGFEGMNLVKRHRLVYHLLREELDTGLHAL-SIVSKTPSESP 159

Query: 175 AQN 177
           +++
Sbjct: 160 SKD 160

The following BLAST results are available for this feature:

BLAST of CSPI05G08270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF68	1.7e-32	54.43	Protein BOLA4, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BO...	[more]

BLAST of CSPI05G08270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KRR5	4.0e-88	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G172860 PE=3 SV=1	[more]
A0A1S3CT29	1.9e-82	94.32	protein BOLA4, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC1035046...	[more]
A0A5A7UJH5	9.4e-82	93.75	Protein BOLA4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00900 ...	[more]
A0A6J1DZG6	3.7e-70	82.95	protein BOLA4, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1K139	2.0e-68	80.68	protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of CSPI05G08270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143637.1	8.2e-88	100.00	protein BOLA4, chloroplastic/mitochondrial [Cucumis sativus] >KGN50406.1 hypothe...	[more]
XP_008467240.1	3.9e-82	94.32	PREDICTED: protein BOLA4, chloroplastic/mitochondrial [Cucumis melo]	[more]
KAA0055158.1	1.9e-81	93.75	protein BOLA4 [Cucumis melo var. makuwa]	[more]
XP_038906458.1	1.5e-78	89.27	protein BOLA4, chloroplastic/mitochondrial [Benincasa hispida]	[more]
XP_022159690.1	7.7e-70	82.95	protein BOLA4, chloroplastic/mitochondrial [Momordica charantia]	[more]

BLAST of CSPI05G08270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17560.1	1.2e-33	54.43	BolA-like family protein	[more]
AT4G26500.1	2.1e-04	31.03	chloroplast sulfur E	[more]
AT1G55805.1	2.7e-04	28.46	BolA-like family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002634	BolA protein	PFAM	PF01722	BolA	coord: 119..171 e-value: 3.2E-13 score: 49.8
None	No IPR available	GENE3D	3.10.20.90		coord: 72..176 e-value: 2.2E-23 score: 84.6
None	No IPR available	PANTHER	PTHR46230:SF4	PROTEIN BOLA4, CHLOROPLASTIC/MITOCHONDRIAL	coord: 25..176
None	No IPR available	PANTHER	PTHR46230	FAMILY NOT NAMED	coord: 25..176
IPR036065	BolA-like superfamily	SUPERFAMILY	82657	BolA-like	coord: 82..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G08270.1	CSPI05G08270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion