CSPI05G07400 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G07400
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein kinase domain-containing protein
LocationChr5: 6449569 .. 6452155 (-)
RNA-Seq ExpressionCSPI05G07400
SyntenyCSPI05G07400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAAACAAAACAATTTTCACTTTCAAATTCCAATCATTATAAACTCTCCCTCTCTCTCTCTCTGTTCATCACAAACTTACAATTCTTTTTTCAACATACCCACATGCTCAAACTAATCCTTCCCCTTGCTTCCGCCACCCTTTTCCTTCTCCTCCTAATCACCATCTTCCTTCTTCGCCTTAAGCTCTCTAAAAATCACGACATCGAGAAATCCTCCACCACCCACAACAACAACAACACCAAAACAGACCACACCCCCCCTCCGCCCCCGCCCGAGCTCACCACTTTTGAGGGCGGCGAAGATTTGACCATTGACGAGATTTTAGAGGCTCCCGGAGAAGTGATCGGAAAATCCCACTACGGAACTCTCTACAAGGCTGTCTTACAGACCACCCAAACCATCCGGCTACTTAGATTTCTCCGGCCGGCCGCTGCCGGATGTGACGACCAACACGACCTCGTTCGGCTCCTGGGTTCGGTTCGGCATCCGAATTTGGTTCCGTTGCTGGGATTCTACGCCGGTGGGAGAGGTGAGAAGCTTTTGGTTCATCCGTTCTACGAACATGGGAATTTGGCGGAGTTCATTACAGGTAATTTTCAATATGATTATTTAAAAAAAATTTCTGTTTCTTTCTCGAGAATGTGATAAGGAAAATTAGATGAATTTGTCGTAAAAACTATAAAATAAAATTTAAAAAGTTATTATTGTGGAAGAGAAAATTTTTAGAACATTAAACAATAATGTGAGTTTTGGAAATAAGATAATTATAATTACGTATCAGATTATAAATGGAAAACATAGATATTTGATATTTAAACTATATATATAATTTTTAAAAATATAATTTAGTCTATCTAATTTCAAATGTCTAATTTTAATTCTTATTTTTAATAAATCTCAAATTCGGTATATAAAATACAATTTTTGTCAAAACTATTTAAACAATAACAATAATTTTGGGTAAGAAAATACAAATATATAAATACATTTGCAAATTTTAAATCAAACAAAAATAAATTGAATAAATATCTTAATAAAATTTTAAAAAATAGTATTAATTAGGAATTAAATTAGTATGGTAATATTAGGAATTACGTGAGATGTCGGCAACTATATATATATATATATATATATTGTGGATTGTAAAGAAAAGAAATTAGAGATGGCGACGATTCTTCATTTGTCATTTTCTTTACTATTTTTCCATTTATTTCTTCCTTAAATCTATATTTCTTTTTCTTTTCCATATATATTTGTAAAAATTTTCCAACTATTTCCATTATTTTTTACCTTAAAATTGCACTTTACTTTTTTTTTTCACAATATATTCCTTTTTAATCTTCCTAAAAAAACCATTTTGCTATATTTAAAAATATTTATCTCCTTTATTTTTTTGGATTGCAAGGATATATTTAACTTAATACATACTTGATTTGCTTGTAGATGATTTTCTTGGTTATAAATTTTAAAATAGGTAATTTATTACCTAAAGTTTATATTTTTCTTTTCCATAAGAGTATTAAAAAGTTAAATTACTTTATGATCTGAAGGATGTGGTGTGGACCAAATGAAATTCCATTCTTCAAAAAAGATTCTTCTTTTGGTATATTTCCTACATTTTTTTTTAAAGAAAAAAAAGATCATACATATATCTTAACAATTCAATGATATTAGATTTAAAATAAGAAAAAGAAAAAGTTATGTCCACCTATCTTAACATATGAAATGAGATGTATTCAATTCATTGACATTTCTCTTTCATTTGCCTCATCTTTTGATGTCATTTACATTTCTATCTCTTCATTTACAATTCTCTAAATTCAATTCTCATATTAGGATTTTTAAAGTTATAATTAATTGAGTCTATCCCTTCACTATAATGAAGTTTGAAGATTGAGAGAAGTAGAAATAACTTTTAAAATTCAAGTTTGAAAAATTAAGGGGAAAAAGAATTAATTTGTTTTAAGCTAAAAATGTTAGAGATTAAAAGATTAAAATTTTTTATGAATTTAAACTAATTTTGTTTTGTATAAAACAGATAAGGAATCAAAAAACGGGGTGGTGATTTGCAAAATCGCCATTGGAATCGCAAGAGCTCTACATCATCTTCACAATGGATTACAAAAACCAATCCCCCATGGAAATCTCAACTCCAAGAACGTTCTTCTCGATCAAAATTTCCACCCCCACGTCTCCGATTTCGGCCTTCATCTCCTCCTCAACTCCCCCGCAACTCGACAAATGCTCCAACTTTCCGCCTCCTCTGGCTACAAGGCCCCAGAACTTATCAATATGACCGACGCTGATGAACTCACCGACGTCTACAGCTACGGAAAGATCTTGCTCGAGTTGCTGATGTCCTCCAAAGACCCAACGGTTCGAAACCCGGCTGCCACCGGTGATTCCGGCGTCTCTCAAGAGCCGATTCTCCAATGCCTTGTTCAGATCGGAGAGGATTGTTGTCGTTCTTCGCCCAATCTTCGGCCGGATTTCAGGGAAGTGATTGCTAAGATTGAAGAAATCGGAAGAATTGAGATGAATTAACTCATGAATTTATTTTTTTGGGGATGAGTTTGGAGCAATTG

mRNA sequence

CCCAAACAAAACAATTTTCACTTTCAAATTCCAATCATTATAAACTCTCCCTCTCTCTCTCTCTGTTCATCACAAACTTACAATTCTTTTTTCAACATACCCACATGCTCAAACTAATCCTTCCCCTTGCTTCCGCCACCCTTTTCCTTCTCCTCCTAATCACCATCTTCCTTCTTCGCCTTAAGCTCTCTAAAAATCACGACATCGAGAAATCCTCCACCACCCACAACAACAACAACACCAAAACAGACCACACCCCCCCTCCGCCCCCGCCCGAGCTCACCACTTTTGAGGGCGGCGAAGATTTGACCATTGACGAGATTTTAGAGGCTCCCGGAGAAGTGATCGGAAAATCCCACTACGGAACTCTCTACAAGGCTGTCTTACAGACCACCCAAACCATCCGGCTACTTAGATTTCTCCGGCCGGCCGCTGCCGGATGTGACGACCAACACGACCTCGTTCGGCTCCTGGGTTCGGTTCGGCATCCGAATTTGGTTCCGTTGCTGGGATTCTACGCCGGTGGGAGAGGTGAGAAGCTTTTGGTTCATCCGTTCTACGAACATGGGAATTTGGCGGAGTTCATTACAGATAAGGAATCAAAAAACGGGGTGGTGATTTGCAAAATCGCCATTGGAATCGCAAGAGCTCTACATCATCTTCACAATGGATTACAAAAACCAATCCCCCATGGAAATCTCAACTCCAAGAACGTTCTTCTCGATCAAAATTTCCACCCCCACGTCTCCGATTTCGGCCTTCATCTCCTCCTCAACTCCCCCGCAACTCGACAAATGCTCCAACTTTCCGCCTCCTCTGGCTACAAGGCCCCAGAACTTATCAATATGACCGACGCTGATGAACTCACCGACGTCTACAGCTACGGAAAGATCTTGCTCGAGTTGCTGATGTCCTCCAAAGACCCAACGGTTCGAAACCCGGCTGCCACCGGTGATTCCGGCGTCTCTCAAGAGCCGATTCTCCAATGCCTTGTTCAGATCGGAGAGGATTGTTGTCGTTCTTCGCCCAATCTTCGGCCGGATTTCAGGGAAGTGATTGCTAAGATTGAAGAAATCGGAAGAATTGAGATGAATTAACTCATGAATTTATTTTTTTGGGGATGAGTTTGGAGCAATTG

Coding sequence (CDS)

ATGCTCAAACTAATCCTTCCCCTTGCTTCCGCCACCCTTTTCCTTCTCCTCCTAATCACCATCTTCCTTCTTCGCCTTAAGCTCTCTAAAAATCACGACATCGAGAAATCCTCCACCACCCACAACAACAACAACACCAAAACAGACCACACCCCCCCTCCGCCCCCGCCCGAGCTCACCACTTTTGAGGGCGGCGAAGATTTGACCATTGACGAGATTTTAGAGGCTCCCGGAGAAGTGATCGGAAAATCCCACTACGGAACTCTCTACAAGGCTGTCTTACAGACCACCCAAACCATCCGGCTACTTAGATTTCTCCGGCCGGCCGCTGCCGGATGTGACGACCAACACGACCTCGTTCGGCTCCTGGGTTCGGTTCGGCATCCGAATTTGGTTCCGTTGCTGGGATTCTACGCCGGTGGGAGAGGTGAGAAGCTTTTGGTTCATCCGTTCTACGAACATGGGAATTTGGCGGAGTTCATTACAGATAAGGAATCAAAAAACGGGGTGGTGATTTGCAAAATCGCCATTGGAATCGCAAGAGCTCTACATCATCTTCACAATGGATTACAAAAACCAATCCCCCATGGAAATCTCAACTCCAAGAACGTTCTTCTCGATCAAAATTTCCACCCCCACGTCTCCGATTTCGGCCTTCATCTCCTCCTCAACTCCCCCGCAACTCGACAAATGCTCCAACTTTCCGCCTCCTCTGGCTACAAGGCCCCAGAACTTATCAATATGACCGACGCTGATGAACTCACCGACGTCTACAGCTACGGAAAGATCTTGCTCGAGTTGCTGATGTCCTCCAAAGACCCAACGGTTCGAAACCCGGCTGCCACCGGTGATTCCGGCGTCTCTCAAGAGCCGATTCTCCAATGCCTTGTTCAGATCGGAGAGGATTGTTGTCGTTCTTCGCCCAATCTTCGGCCGGATTTCAGGGAAGTGATTGCTAAGATTGAAGAAATCGGAAGAATTGAGATGAATTAA

Protein sequence

MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHNNNNTKTDHTPPPPPPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN*
Homology
BLAST of CSPI05G07400 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-44
Identity = 107/299 (35.79%), Postives = 164/299 (54.85%), Query Frame = 0

Query: 58  ELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQH 117
           +L  F+GGE+LT+D++L A G+V+ K+ YGT+YKA L     I  LR LR     C D+ 
Sbjct: 356 KLVVFQGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI-ALRLLREGT--CKDRS 415

Query: 118 D---LVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITD----KESKNGV 177
               ++R LG +RH NLVPL  FY G RGEKLL++ +  + +L + + +    K + N  
Sbjct: 416 SCLPVIRQLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWA 475

Query: 178 VICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQ 237
              KIA+GIAR L +LH G + PI HGN+ SKNVL+D  F   +++FGL  ++      +
Sbjct: 476 RRHKIALGIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADE 535

Query: 238 MLQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKD-------------PTVR 297
           ++  + S GYKAPEL  M   +  +DVY++G +LLE+LM  K              P++ 
Sbjct: 536 IVSQAKSDGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLV 595

Query: 298 NPAATGDSGVS----------QEPILQCLV---QIGEDCCRSSPNLRPDFREVIAKIEE 324
             A   ++ +           + P+ + LV   ++   CC     +RP   EV+ ++EE
Sbjct: 596 KAAVLEETTMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of CSPI05G07400 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-32
Identity = 106/352 (30.11%), Postives = 171/352 (48.58%), Query Frame = 0

Query: 4   LILPLASATLFLLLLITIFLLRLKLSKNHDIEK-------SSTTHNNNNTKTDHTPPPPP 63
           +IL    A L +LLL+   LL   + K   +++       S  T +     T        
Sbjct: 459 VILIAIGALLAILLLLCCILLCCLIKKRAALKQKDGKDKTSEKTVSAGVAGTASAGGEMG 518

Query: 64  PELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQ 123
            +L  F+G    T D++L A  E++GKS YGT YKA L+    + + R       G  + 
Sbjct: 519 GKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEF 578

Query: 124 HDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGV---VIC 183
              V  LG +RH NL+ L  +Y G +GEKLLV  +   G+L+ F+  +  +  +      
Sbjct: 579 EGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRM 638

Query: 184 KIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQ 243
           KIA GI+R L HLH+   + + H NL + N+LLD+  + H++D+GL  L+ + A   ++ 
Sbjct: 639 KIAKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIA 698

Query: 244 LSASSGYKAPELINMTDADELTDVYSYGKILLELL--MSSKDPTVRNPAATGDSGVSQE- 303
            + + GY+APE   + +A   TDVYS G I+LELL   S  +PT         + + +E 
Sbjct: 699 TAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEE 758

Query: 304 --------PILQCLVQIGED----------CCRSSPNLRPDFREVIAKIEEI 325
                    +++    +G++          C   SP  RP+  +V+ ++EEI
Sbjct: 759 WTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

BLAST of CSPI05G07400 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 96/284 (33.80%), Postives = 145/284 (51.06%), Query Frame = 0

Query: 3   KLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHNNNNTKTDHTPPPPPPE---- 62
           K I+ +AS  L +++LI + +L   L K  +  K+             T      E    
Sbjct: 408 KDIILIASGALLIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGE 467

Query: 63  ----LTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCD 122
               L  F+G    T D++L A  E++GKS YGT+YKA L+    + + R          
Sbjct: 468 TGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKRLREKITKSQK 527

Query: 123 DQHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESK---NGVV 182
           +  + + +LG +RHPNL+ L  +Y G +GEKL+V  +   G+LA F+  +      N   
Sbjct: 528 EFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHINWPT 587

Query: 183 ICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQM 242
              +  G+AR L +LH      I HGNL S NVLLD+N    +SD+GL  L+ + A   +
Sbjct: 588 RMSLIKGMARGLFYLHT--HANIIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSSV 647

Query: 243 LQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPT 276
           +  + + GY+APEL  +  A+  TDVYS G I+LELL + K P+
Sbjct: 648 IATAGALGYRAPELSKLKKANTKTDVYSLGVIILELL-TGKSPS 688

BLAST of CSPI05G07400 vs. ExPASy Swiss-Prot
Match: Q9LP77 (Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=RKL1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 98/324 (30.25%), Postives = 156/324 (48.15%), Query Frame = 0

Query: 32  HDIEKSSTTHNNNNTKTDHTPPPPPPELTTFEGGEDL-TIDEILEAPGEVIGKSHYGTLY 91
           + +  ++      N K      P   +L  F     +  ++++L A  EV+GK  +GT Y
Sbjct: 327 YSVSAAAAAAMTGNGKASEGNGPATKKLVFFGNATKVFDLEDLLRASAEVLGKGTFGTAY 386

Query: 92  KAVLQTTQTIRLLRFLRPAAAGCDDQHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHP 151
           KAVL     + + R L+       +  + + L+G++ H NLVPL  +Y   R EKLLV+ 
Sbjct: 387 KAVLDAVTVVAVKR-LKDVMMADKEFKEKIELVGAMDHENLVPLRAYYF-SRDEKLLVYD 446

Query: 152 FYEHGNLAEFI-----TDKESKNGVVICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVL 211
           F   G+L+  +       +   N  V  +IAIG AR L +LH+       HGN+ S N+L
Sbjct: 447 FMPMGSLSALLHGNRGAGRSPLNWDVRSRIAIGAARGLDYLHS-QGTSTSHGNIKSSNIL 506

Query: 212 LDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGYKAPELINMTDADELTDVYSYGKILL 271
           L ++    VSDFGL  L+ S AT      + ++GY+APE+ +     +  DVYS+G +LL
Sbjct: 507 LTKSHDAKVSDFGLAQLVGSSATNP----NRATGYRAPEVTDPKRVSQKGDVYSFGVVLL 566

Query: 272 ELLMSSKDP--TVRNPAATG------------------DS-----GVSQEPILQCLVQIG 325
           EL+ + K P  +V N                       DS        +E ++  +VQ+G
Sbjct: 567 ELI-TGKAPSNSVMNEEGVDLPRWVKSVARDEWRREVFDSELLSLATDEEEMMAEMVQLG 626

BLAST of CSPI05G07400 vs. ExPASy Swiss-Prot
Match: Q9SUQ3 (Probable inactive receptor kinase At4g23740 OS=Arabidopsis thaliana OX=3702 GN=At4g23740 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-29
Identity = 74/222 (33.33%), Postives = 126/222 (56.76%), Query Frame = 0

Query: 59  LTTFEG-GEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQH 118
           L+ FEG      ++++L A  EV+GK  +GT YKAVL+   ++ + R L+  AAG  D  
Sbjct: 320 LSFFEGCNYSFDLEDLLRASAEVLGKGTFGTTYKAVLEDATSVAVKR-LKDVAAGKRDFE 379

Query: 119 DLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVI----- 178
             + ++G ++H N+V L  +Y   + EKL+V+ ++  G++A  +     +N + +     
Sbjct: 380 QQMEIIGGIKHENVVELKAYYY-SKDEKLMVYDYFSRGSVASLLHGNRGENRIPLDWETR 439

Query: 179 CKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQML 238
            KIAIG A+ +  +H      + HGN+ S N+ L+   +  VSD GL  +++  A     
Sbjct: 440 MKIAIGAAKGIARIHKENNGKLVHGNIKSSNIFLNSESNGCVSDLGLTAVMSPLAP---- 499

Query: 239 QLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDP 275
            +S  +GY+APE+ +   + +L+DVYS+G +LLELL + K P
Sbjct: 500 PISRQAGYRAPEVTDTRKSSQLSDVYSFGVVLLELL-TGKSP 534

BLAST of CSPI05G07400 vs. ExPASy TrEMBL
Match: A0A0A0KKU6 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167070 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.4e-185
Identity = 329/330 (99.70%), Postives = 329/330 (99.70%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHNNNNTKTDHTPPPPPPELT 60
           MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTT NNNNTKTDHTPPPPPPELT
Sbjct: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTRNNNNTKTDHTPPPPPPELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV 120
           TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV 120

Query: 121 RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA 180
           RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA
Sbjct: 121 RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA 180

Query: 181 RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY 240
           RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY
Sbjct: 181 RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY 240

Query: 241 KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG 300
           KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG
Sbjct: 241 KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG 300

Query: 301 EDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           EDCCRSSPNLRPDFREVIAKIEEIGRIEMN
Sbjct: 301 EDCCRSSPNLRPDFREVIAKIEEIGRIEMN 330

BLAST of CSPI05G07400 vs. ExPASy TrEMBL
Match: A0A5A7TJN4 (Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00210 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 299/332 (90.06%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH-NNNNTKTDHTPPPPPPEL 60
           MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH  NNN KTDHTP PPPPEL
Sbjct: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHKTNNNNKTDHTPLPPPPEL 60

Query: 61  TTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDL 120
           TTFEGG+DLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAG +DQHDL
Sbjct: 61  TTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGGEDQHDL 120

Query: 121 VRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK-ESKNGVVICKIAIG 180
           VR LGSVRH NLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFI D  ESKN  VICKIAIG
Sbjct: 121 VRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIRDDGESKNWEVICKIAIG 180

Query: 181 IARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASS 240
           IARALHHLH+GLQKPI HGNLNSKNVLLDQN  PHVSDFGLHLLLNS ATR+MLQ+SASS
Sbjct: 181 IARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREMLQVSASS 240

Query: 241 GYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQ 300
           GYKAPELINMT ADE TDVYSYGKILLELL+S+K+PT+RNP ATGDSGVS E IL+CLVQ
Sbjct: 241 GYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVATGDSGVSGEQILRCLVQ 300

Query: 301 IGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           IGEDCCRSSPNLRPDFREVIAKIEEI RIE N
Sbjct: 301 IGEDCCRSSPNLRPDFREVIAKIEEIRRIEKN 332

BLAST of CSPI05G07400 vs. ExPASy TrEMBL
Match: A0A1S3AT53 (putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103482568 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 299/332 (90.06%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH-NNNNTKTDHTPPPPPPEL 60
           MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH  NNN KTDHTP PPPPEL
Sbjct: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHKTNNNNKTDHTPLPPPPEL 60

Query: 61  TTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDL 120
           TTFEGG+DLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAG +DQHDL
Sbjct: 61  TTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGGEDQHDL 120

Query: 121 VRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK-ESKNGVVICKIAIG 180
           VR LGSVRH NLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFI D  ESKN  VICKIAIG
Sbjct: 121 VRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIRDDGESKNWEVICKIAIG 180

Query: 181 IARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASS 240
           IARALHHLH+GLQKPI HGNLNSKNVLLDQN  PHVSDFGLHLLLNS ATR+MLQ+SASS
Sbjct: 181 IARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREMLQVSASS 240

Query: 241 GYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQ 300
           GYKAPELINMT ADE TDVYSYGKILLELL+S+K+PT+RNP ATGDSGVS E IL+CLVQ
Sbjct: 241 GYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVATGDSGVSGEQILRCLVQ 300

Query: 301 IGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           IGEDCCRSSPNLRPDFREVIAKIEEI RIE N
Sbjct: 301 IGEDCCRSSPNLRPDFREVIAKIEEIRRIEKN 332

BLAST of CSPI05G07400 vs. ExPASy TrEMBL
Match: A0A6J1ELS2 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.2e-118
Identity = 232/337 (68.84%), Postives = 268/337 (79.53%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITI--FLLRLKLS---KNHDIEKSSTTHNNNNTKTDHTPPPP 60
           MLK +L +ASA +F+LLLI+I  F  R KLS     HD+E+     N+ + K +HTPPP 
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPC---NDEHNKKEHTPPPS 60

Query: 61  PPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDD 120
           PP L TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKA LQ+TQ  RLLRFLRP A G   
Sbjct: 61  PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGG--G 120

Query: 121 QHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK--ESKNGVVIC 180
           ++D+VR LGS+RH NLVP+LGFYAGGRGE+LLVHPFY  GNLAE I ++  ESKN  VI 
Sbjct: 121 EYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVIN 180

Query: 181 KIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQ 240
           KI++GIARAL HLHNGLQKPIPHGNL SKN+LLD NF PHVSDFGLHLLLN+ ATR+ML+
Sbjct: 181 KISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLK 240

Query: 241 LSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPIL 300
           +SA+SGYKAPELINMTDADE TDV+SYGKILLELL + +          GD G S+E  L
Sbjct: 241 VSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEE-----TGGDGGDCGGSEERTL 300

Query: 301 QCLVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           +CL+QIGE CC SSP+ RPDF+EVIAKI EIGRIEM+
Sbjct: 301 RCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 327

BLAST of CSPI05G07400 vs. ExPASy TrEMBL
Match: A0A6J1I3Q5 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.4e-117
Identity = 232/338 (68.64%), Postives = 270/338 (79.88%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITI---FLLRLKLS---KNHDIEKSSTTHNNNNTKTDHTPPP 60
           MLK IL +ASA +F+LLLI+I   F  R KLS     HD+E+  T   + N K +HTPPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCT---DQNKKKEHTPPP 60

Query: 61  PPPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCD 120
            PP L TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKA LQ+TQ+ RLLRFLRP A G  
Sbjct: 61  SPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGG-- 120

Query: 121 DQHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIT--DKESKNGVVI 180
            ++D+VR LGS+RH NLVP+LGFYAGGRGE+LLVHPFY  GNLA+ I   + E+KN  VI
Sbjct: 121 GEYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVI 180

Query: 181 CKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQML 240
            KI++GIARAL HLHNGLQKPIPHGNL SKN+LLD NF PHVSDFGLHLLLN+ ATR+ML
Sbjct: 181 NKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML 240

Query: 241 QLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPI 300
           Q+SA+SGYKAPELINM DADE TD++SYGKILLELL S+K+         GD GVS+E  
Sbjct: 241 QVSAASGYKAPELINMNDADERTDIFSYGKILLELL-SAKE----TGGDGGDCGVSEERT 300

Query: 301 LQCLVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           L+C++QIGE C  SSP+LRP+F+EVIAKI EIGRIEM+
Sbjct: 301 LRCILQIGEACSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

BLAST of CSPI05G07400 vs. NCBI nr
Match: XP_004143741.1 (putative kinase-like protein TMKL1 [Cucumis sativus] >KGN50315.1 hypothetical protein Csa_000024 [Cucumis sativus])

HSP 1 Score: 656.0 bits (1691), Expect = 1.7e-184
Identity = 329/330 (99.70%), Postives = 329/330 (99.70%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHNNNNTKTDHTPPPPPPELT 60
           MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTT NNNNTKTDHTPPPPPPELT
Sbjct: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTRNNNNTKTDHTPPPPPPELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV 120
           TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV 120

Query: 121 RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA 180
           RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA
Sbjct: 121 RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGVVICKIAIGIA 180

Query: 181 RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY 240
           RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY
Sbjct: 181 RALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASSGY 240

Query: 241 KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG 300
           KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG
Sbjct: 241 KAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQIG 300

Query: 301 EDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           EDCCRSSPNLRPDFREVIAKIEEIGRIEMN
Sbjct: 301 EDCCRSSPNLRPDFREVIAKIEEIGRIEMN 330

BLAST of CSPI05G07400 vs. NCBI nr
Match: XP_008437011.1 (PREDICTED: putative kinase-like protein TMKL1 [Cucumis melo] >KAA0043482.1 putative kinase-like protein TMKL1 [Cucumis melo var. makuwa])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 299/332 (90.06%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH-NNNNTKTDHTPPPPPPEL 60
           MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTH  NNN KTDHTP PPPPEL
Sbjct: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHKTNNNNKTDHTPLPPPPEL 60

Query: 61  TTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDL 120
           TTFEGG+DLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAG +DQHDL
Sbjct: 61  TTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGGEDQHDL 120

Query: 121 VRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK-ESKNGVVICKIAIG 180
           VR LGSVRH NLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFI D  ESKN  VICKIAIG
Sbjct: 121 VRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIRDDGESKNWEVICKIAIG 180

Query: 181 IARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASS 240
           IARALHHLH+GLQKPI HGNLNSKNVLLDQN  PHVSDFGLHLLLNS ATR+MLQ+SASS
Sbjct: 181 IARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREMLQVSASS 240

Query: 241 GYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLVQ 300
           GYKAPELINMT ADE TDVYSYGKILLELL+S+K+PT+RNP ATGDSGVS E IL+CLVQ
Sbjct: 241 GYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVATGDSGVSGEQILRCLVQ 300

Query: 301 IGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           IGEDCCRSSPNLRPDFREVIAKIEEI RIE N
Sbjct: 301 IGEDCCRSSPNLRPDFREVIAKIEEIRRIEKN 332

BLAST of CSPI05G07400 vs. NCBI nr
Match: XP_038877112.1 (putative kinase-like protein TMKL1 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 5.5e-138
Identity = 264/333 (79.28%), Postives = 292/333 (87.69%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSKNHDIEKSSTTHNNNNTKTDH-TPPPPPPEL 60
           MLKLIL +ASAT+F+LLLI+IFL RLKLSKNHDIE  S   +  N KT+H  PPPPPPEL
Sbjct: 1   MLKLILLVASATIFILLLISIFLFRLKLSKNHDIENPS---SKKNKKTEHRPPPPPPPEL 60

Query: 61  TTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDL 120
           TTFEGGE LTIDEILEAPGEVIGKSHYGTLYKA LQ+TQT RLLRFLRPAA+G DDQ D+
Sbjct: 61  TTFEGGEYLTIDEILEAPGEVIGKSHYGTLYKATLQSTQTTRLLRFLRPAASGGDDQDDV 120

Query: 121 VRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFI--TDKESKNGVVICKIAI 180
           VRLLGS+RH NLVPLLGFYAGGRGEKLLVHPFYE GNLAEFI  ++ ESKN   I KIAI
Sbjct: 121 VRLLGSIRHRNLVPLLGFYAGGRGEKLLVHPFYEQGNLAEFIRVSETESKNWEAIYKIAI 180

Query: 181 GIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSAS 240
           GIA+AL HLHNGLQ+PIPHGNL SKN+LLD+N  PH+SDFGLHLLLN+ ATR+MLQ+SA+
Sbjct: 181 GIAKALDHLHNGLQRPIPHGNLKSKNILLDRNLDPHISDFGLHLLLNAAATREMLQVSAA 240

Query: 241 SGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPILQCLV 300
           +GYKAPELI MTDADE TDVYSYGKILLELL S+K+PT+RNP AT   GVS+E IL+CLV
Sbjct: 241 AGYKAPELIKMTDADERTDVYSYGKILLELL-SAKEPTIRNPVAT---GVSEERILRCLV 300

Query: 301 QIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           QIGE CCRSSPNLR DFREVIAKIEEIGRIEM+
Sbjct: 301 QIGEACCRSSPNLRLDFREVIAKIEEIGRIEMD 326

BLAST of CSPI05G07400 vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 440.3 bits (1131), Expect = 1.5e-119
Identity = 237/337 (70.33%), Postives = 273/337 (81.01%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITI--FLLRLKLS---KNHDIEKSSTTHNNNNTKTDHTPPPP 60
           MLK IL +ASA +F+LLLI+I  F  R KLS     HD+E+     N+ + K +HTPPP 
Sbjct: 48  MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPC---NDEHNKKEHTPPPS 107

Query: 61  PPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDD 120
           PP L TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKA LQ+TQ+ RLLRFLRP A G   
Sbjct: 108 PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGG--G 167

Query: 121 QHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK--ESKNGVVIC 180
           ++D+VR LGS+RH NLVP+LGFYAGGRGE+LLVHPFY  GNLAE I ++  ESKN  VI 
Sbjct: 168 EYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVIN 227

Query: 181 KIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQ 240
           KI++GIARAL HLHNGLQKPIPHGNL SKN+LLD NF PHVSDFGLHLLLN+ ATR+MLQ
Sbjct: 228 KISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQ 287

Query: 241 LSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPIL 300
           +SA+SGYKAPELINM+DADE TDV+SYGKILLELL S+K+         GD GVS+E  L
Sbjct: 288 VSAASGYKAPELINMSDADERTDVFSYGKILLELL-SAKE----TGGDGGDCGVSEERTL 347

Query: 301 QCLVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           +CL+QIGE CC SSP+LRPDF+EVIAKI EIGRIEM+
Sbjct: 348 RCLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMD 374

BLAST of CSPI05G07400 vs. NCBI nr
Match: KAG6579628.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434.5 bits (1116), Expect = 8.3e-118
Identity = 233/338 (68.93%), Postives = 268/338 (79.29%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITI---FLLRLKLS---KNHDIEKSSTTHNNNNTKTDHTPPP 60
           MLK IL +ASA +F+LLLI+I   F  R KLS     HD+E+     N+ + K +HTPPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPC---NDEHNKKEHTPPP 60

Query: 61  PPPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCD 120
            PP L TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKA LQ TQ+ RLLRFLRP A G  
Sbjct: 61  SPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGG-- 120

Query: 121 DQHDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDK--ESKNGVVI 180
            ++D+VR LGS+RH NLVP+LGFYAGGRGE+LLVHPFY  GNLAE I ++  ESKN  VI
Sbjct: 121 GEYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVI 180

Query: 181 CKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQML 240
            KI++GIARAL HLHNGLQKPIPHGNL SKN+LLD NF PHVSDFGLHLLLN+ ATR+ML
Sbjct: 181 NKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML 240

Query: 241 QLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSGVSQEPI 300
           ++SA+SGYKAPELINMTDADE TDV+SYGKILLELL + +          GD G S+E  
Sbjct: 241 KVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEE-----TGGDGGDCGGSEERT 300

Query: 301 LQCLVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 331
           L+CL+QIGE CC SSP+ RPDF+EVIAKI EIGRIEM+
Sbjct: 301 LRCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 328

BLAST of CSPI05G07400 vs. TAIR 10
Match: AT5G07620.1 (Protein kinase superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.4e-77
Identity = 170/365 (46.58%), Postives = 225/365 (61.64%), Query Frame = 0

Query: 1   MLKLILPLASATLFLLLLITIFLLRLKLSK----NHDIEKSSTTHNNNNTKTDHTPPPPP 60
           +L L+L ++ +   +++   IFL R + S      +D+E  S  HN     ++       
Sbjct: 6   LLTLLLGVSLSAFLIVIFFIIFLRRKESSSTESDQYDVE--SLDHNKQGFSSE------T 65

Query: 61  PELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQ 120
            EL  F+GGEDLTI +IL+APGEVIGKS YGTLYKA LQ +  IR+LRFLRP      D 
Sbjct: 66  EELVIFQGGEDLTICDILDAPGEVIGKSSYGTLYKASLQRSGKIRVLRFLRPVCTVRSDS 125

Query: 121 HD---LVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIT--DKESKNGVV 180
            +   ++  LG VRH NLVPLLGFYAG RGEKL+VHPF+  GNL++FI   D ES+  + 
Sbjct: 126 KEFNGIIETLGFVRHENLVPLLGFYAGNRGEKLMVHPFFGSGNLSDFIRSGDDESRKWIN 185

Query: 181 ICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQM 240
           I +I IGI++AL HLH G+QKPI HGNL SKNVLL  +F P +SDFGLHLLLN  A +++
Sbjct: 186 ILRITIGISKALDHLHTGMQKPIVHGNLKSKNVLLSSSFEPRISDFGLHLLLNLSAGQEI 245

Query: 241 LQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATG-------- 300
           L +SA+ GYKAPELI M D  + +DVYS G I+LE L+S K+P   N  ATG        
Sbjct: 246 LDVSAAEGYKAPELIKMKDVSKESDVYSLGVIMLE-LVSGKEPI--NENATGDDEFYLPD 305

Query: 301 ----------------------DSGVSQEPILQCLVQIGEDCCRSSPNLRPDFREVIAKI 327
                                 D  +S+E +L+   Q+   CC  SP+LRP+ ++V+ K+
Sbjct: 306 FMRNAVLDHRLSDLYRPEILGSDDNLSEECVLK-YFQLAMSCCSPSPSLRPNVKQVLRKL 358

BLAST of CSPI05G07400 vs. TAIR 10
Match: AT5G61570.2 (Protein kinase superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 7.5e-77
Identity = 170/360 (47.22%), Postives = 226/360 (62.78%), Query Frame = 0

Query: 1   MLKLILPLASATLFL----LLLITIFLLRLKLSKN---HDIEK--SSTTHNNNNTKTDHT 60
           ++KLIL ++  T FL    +++I IF  R     N   +D+E        ++N ++T+  
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESPYEKQEFSDNGSETEE- 65

Query: 61  PPPPPPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAA 120
                 EL  F GGEDLTI +IL+APGEVIGKS YGTLYKA LQ +  +R+LRFLRP  A
Sbjct: 66  ------ELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCA 125

Query: 121 GCDDQHD---LVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFY-EHGNLAEFIT--DKE 180
              D  +   ++  LG VRH NLVPLLGFY G RGEKL++HPF+   GNL+ FI   D +
Sbjct: 126 VNSDSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKCGDVD 185

Query: 181 SKNGVVICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNS 240
           +     I  I IGIA+AL HLH G+QKPI HGNL SKNVLLD++F P VSDFGLHLLLN 
Sbjct: 186 AHKWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNL 245

Query: 241 PATRQMLQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAAT--- 300
            A +++L+ SA+ GYKAPELI M +  + +DVYS+G I+LE L+S K+PT +NP  +   
Sbjct: 246 AAGQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLE-LVSGKEPTNKNPTGSVLD 305

Query: 301 ------------------GDSGVSQEPILQCLVQIGEDCCRSSPNLRPDFREVIAKIEEI 325
                               +GV++E +L+   Q+   CC  SP LRP F++V+ K+EEI
Sbjct: 306 RNRLSDLYRPEIIRRCLKDGNGVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEEI 356

BLAST of CSPI05G07400 vs. TAIR 10
Match: AT5G61570.1 (Protein kinase superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 170/363 (46.83%), Postives = 226/363 (62.26%), Query Frame = 0

Query: 1   MLKLILPLASATLFL----LLLITIFLLRLKLSKN---HDIEK--SSTTHNNNNTKTDHT 60
           ++KLIL ++  T FL    +++I IF  R     N   +D+E        ++N ++T+  
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESPYEKQEFSDNGSETEE- 65

Query: 61  PPPPPPELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAA 120
                 EL  F GGEDLTI +IL+APGEVIGKS YGTLYKA LQ +  +R+LRFLRP  A
Sbjct: 66  ------ELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCA 125

Query: 121 GCDDQHD---LVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFY-EHGNLAEFIT----- 180
              D  +   ++  LG VRH NLVPLLGFY G RGEKL++HPF+   GNL+ FI      
Sbjct: 126 VNSDSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGG 185

Query: 181 DKESKNGVVICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLL 240
           D ++     I  I IGIA+AL HLH G+QKPI HGNL SKNVLLD++F P VSDFGLHLL
Sbjct: 186 DVDAHKWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLL 245

Query: 241 LNSPATRQMLQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAAT 300
           LN  A +++L+ SA+ GYKAPELI M +  + +DVYS+G I+LE L+S K+PT +NP  +
Sbjct: 246 LNLAAGQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLE-LVSGKEPTNKNPTGS 305

Query: 301 ---------------------GDSGVSQEPILQCLVQIGEDCCRSSPNLRPDFREVIAKI 325
                                  +GV++E +L+   Q+   CC  SP LRP F++V+ K+
Sbjct: 306 VLDRNRLSDLYRPEIIRRCLKDGNGVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKL 359

BLAST of CSPI05G07400 vs. TAIR 10
Match: AT3G24660.1 (transmembrane kinase-like 1 )

HSP 1 Score: 179.5 bits (454), Expect = 4.5e-45
Identity = 107/299 (35.79%), Postives = 164/299 (54.85%), Query Frame = 0

Query: 58  ELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQH 117
           +L  F+GGE+LT+D++L A G+V+ K+ YGT+YKA L     I  LR LR     C D+ 
Sbjct: 356 KLVVFQGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI-ALRLLREGT--CKDRS 415

Query: 118 D---LVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITD----KESKNGV 177
               ++R LG +RH NLVPL  FY G RGEKLL++ +  + +L + + +    K + N  
Sbjct: 416 SCLPVIRQLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWA 475

Query: 178 VICKIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQ 237
              KIA+GIAR L +LH G + PI HGN+ SKNVL+D  F   +++FGL  ++      +
Sbjct: 476 RRHKIALGIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADE 535

Query: 238 MLQLSASSGYKAPELINMTDADELTDVYSYGKILLELLMSSKD-------------PTVR 297
           ++  + S GYKAPEL  M   +  +DVY++G +LLE+LM  K              P++ 
Sbjct: 536 IVSQAKSDGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLV 595

Query: 298 NPAATGDSGVS----------QEPILQCLV---QIGEDCCRSSPNLRPDFREVIAKIEE 324
             A   ++ +           + P+ + LV   ++   CC     +RP   EV+ ++EE
Sbjct: 596 KAAVLEETTMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of CSPI05G07400 vs. TAIR 10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2 )

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-33
Identity = 106/352 (30.11%), Postives = 171/352 (48.58%), Query Frame = 0

Query: 4   LILPLASATLFLLLLITIFLLRLKLSKNHDIEK-------SSTTHNNNNTKTDHTPPPPP 63
           +IL    A L +LLL+   LL   + K   +++       S  T +     T        
Sbjct: 459 VILIAIGALLAILLLLCCILLCCLIKKRAALKQKDGKDKTSEKTVSAGVAGTASAGGEMG 518

Query: 64  PELTTFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQ 123
            +L  F+G    T D++L A  E++GKS YGT YKA L+    + + R       G  + 
Sbjct: 519 GKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEF 578

Query: 124 HDLVRLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFITDKESKNGV---VIC 183
              V  LG +RH NL+ L  +Y G +GEKLLV  +   G+L+ F+  +  +  +      
Sbjct: 579 EGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRM 638

Query: 184 KIAIGIARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQ 243
           KIA GI+R L HLH+   + + H NL + N+LLD+  + H++D+GL  L+ + A   ++ 
Sbjct: 639 KIAKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIA 698

Query: 244 LSASSGYKAPELINMTDADELTDVYSYGKILLELL--MSSKDPTVRNPAATGDSGVSQE- 303
            + + GY+APE   + +A   TDVYS G I+LELL   S  +PT         + + +E 
Sbjct: 699 TAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEE 758

Query: 304 --------PILQCLVQIGED----------CCRSSPNLRPDFREVIAKIEEI 325
                    +++    +G++          C   SP  RP+  +V+ ++EEI
Sbjct: 759 WTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P335436.3e-4435.79Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1... [more]
Q9SCT41.4e-3230.11Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi... [more]
C0LGP91.2e-3133.80Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th... [more]
Q9LP772.3e-3030.25Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q9SUQ31.5e-2933.33Probable inactive receptor kinase At4g23740 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Match NameE-valueIdentityDescription
A0A0A0KKU68.4e-18599.70Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167... [more]
A0A5A7TJN41.0e-16190.06Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3AT531.0e-16190.06putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103482568 PE=4 ... [more]
A0A6J1ELS25.2e-11868.84putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777... [more]
A0A6J1I3Q54.4e-11768.64putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 P... [more]
Match NameE-valueIdentityDescription
XP_004143741.11.7e-18499.70putative kinase-like protein TMKL1 [Cucumis sativus] >KGN50315.1 hypothetical pr... [more]
XP_008437011.12.1e-16190.06PREDICTED: putative kinase-like protein TMKL1 [Cucumis melo] >KAA0043482.1 putat... [more]
XP_038877112.15.5e-13879.28putative kinase-like protein TMKL1 [Benincasa hispida][more]
XP_023520536.11.5e-11970.33putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ... [more]
KAG6579628.18.3e-11868.93putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT5G07620.14.4e-7746.58Protein kinase superfamily protein [more]
AT5G61570.27.5e-7747.22Protein kinase superfamily protein [more]
AT5G61570.11.7e-7646.83Protein kinase superfamily protein [more]
AT3G24660.14.5e-4535.79transmembrane kinase-like 1 [more]
AT3G51740.11.0e-3330.11inflorescence meristem receptor-like kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 79..320
e-value: 1.2E-26
score: 93.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 69..330
e-value: 9.5E-48
score: 164.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..57
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 287..325
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 287..325
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 2..279
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 2..279
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 75..324
score: 24.824263
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 78..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G07400.1CSPI05G07400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity