CSPI05G07300 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G07300 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 163/347 (46.97%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 29  DTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSL-----ESTTATRLSSYRLFVE 88
           D DV EEYANAFRTESY  FWTRVV L+   +  +  S       S+T+ RL SYRLF  
Sbjct: 68  DVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH 127

Query: 89  HLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ 148
           +LLDP   TI  IL           LL  YF  TANA LLC+++LK I HLR K  SL  
Sbjct: 128 NLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESL-- 187

Query: 149 NKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRDKA 208
            K +F+ ++ +   L+ +  E S    P  F+     ++Q+I++GC  LLKRLE  RDK 
Sbjct: 188 -KPKFHSENHNSLALIDQFTEISKWFDP--FIS-SGSRIQLIRSGCLYLLKRLESRRDKT 247

Query: 209 RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKKSRKLAKE 268
           R KLK +    HSS   ++A+T +  V + +H  ALF+AAP  L    K A    KL K 
Sbjct: 248 RAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKLTKT 307

Query: 269 VAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAIDELARQL 328
            A+L+VAAKGTY L+RD DTI RLV R++ E+ H++ MA+FW+ RG  +    +E+AR+L
Sbjct: 308 AARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVAREL 367

Query: 329 NQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISY 371
            +  E+F+++LDELEEH+YLCFMTINRARNL+VKEIL+   P   S+
Sbjct: 368 KRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDPPNCSF 408

BLAST of CSPI05G07300 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 177.2 bits (448), Expect = 3.5e-43
Identity = 130/346 (37.57%), Postives = 184/346 (53.18%), Query Frame = 0

Query: 31  DVGEEYANAFRTESYIDFWTRV--VALNNGDNLTAQVSLESTTAT--RLSSYRLFVEHLL 90
           D  EEY +AFRTESY DFW RV  + L +G  L  +       A   RL SYRLF EHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 91  DPPQPTIKTILTAHLGPN---SCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ 150
           +P Q  +   L +  G         LL  Y++ TANAS LCS +LK I H+RL+   L  
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 151 NKQEFNHD--DSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRD 210
             ++   D   S    +   L        P + +     +++ +Q G   LL+ L+  R 
Sbjct: 153 TLRKLASDVGVSGLADVSAAL------GQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 212

Query: 211 KARDKLKRVRYFQHS-SAGFL--VAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK-- 270
           KAR +++ V   + + S  F+  VA+ A     +  H +A F A P  ++    L ++  
Sbjct: 213 KARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFP--MMSPAWLGERFF 272

Query: 271 -SRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDR-----GG 330
             R   + + QL  AAKGTY LNRD +TI RLVAR+  E EHM  + +  ++       G
Sbjct: 273 SGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAAGAG 332

Query: 331 DKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVV 357
            K   + E+ RQL+++ E+F QQLDELEEHL+LCFMTIN+AR +V+
Sbjct: 333 GKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVM 370

BLAST of CSPI05G07300 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-42
Identity = 129/346 (37.28%), Postives = 183/346 (52.89%), Query Frame = 0

Query: 31  DVGEEYANAFRTESYIDFWTRV--VALNNGDNLTAQVSLESTTAT--RLSSYRLFVEHLL 90
           D  EEY +AFRTESY DFW RV  + L +G  L  +       A   RL SYRLF EHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 91  DPPQPTIKTILTAHLGPN---SCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ 150
           +P Q  +   L +  G         LL  Y++ TANAS LCS +LK I H+RL+   L  
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 151 NKQEFNHD--DSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRD 210
             ++   D   S    +   L        P + +     +++ +Q G   LL+ L+  R 
Sbjct: 153 TLRKLASDVGVSGLADVSAAL------GQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 212

Query: 211 KARDKLKRVRYFQHS-SAGFL--VAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK-- 270
           KAR +++ V   + + S  F+  VA+ A     +  H +A F A P  ++    L ++  
Sbjct: 213 KARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFP--MMSPAWLGERFF 272

Query: 271 -SRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDR-----GG 330
             R   + + QL  AAKGTY LNRD +TI RLVAR+  E EHM  + +  ++       G
Sbjct: 273 SGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAAGAG 332

Query: 331 DKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVV 357
            K   + E+ RQL+++ E+F QQLDELEEHL+LCFMT N+AR +V+
Sbjct: 333 GKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVM 370

BLAST of CSPI05G07300 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 1.3e-24
Identity = 109/364 (29.95%), Postives = 174/364 (47.80%), Query Frame = 0

Query: 32  VGEEYANAFRTESYIDFWTRV---VALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLDP 91
           V EEY  AFRT SY++  T+    + + +   L++  S   ++++ LS +  F ++LLDP
Sbjct: 36  VNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLLDP 95

Query: 92  PQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ----N 151
           PQ T    L A +  +S   L+  +F  ++ A  +C  +L+ +  +++  + + +     
Sbjct: 96  PQET----LDALMQDSSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKIG 155

Query: 152 KQEFNHD---DSHFKQLLVRLF-EFSNDSTPNSFVPYCM----EQVQIIQNGCSKLLKRL 211
           K+  N     +   + L   +F E S  +   +  P C      Q +I+ +  S LL +L
Sbjct: 156 KRVCNGAKTLECSPEMLCALIFQELSRFAALKN--PLCRIVNEAQFRIVHDANSDLLTKL 215

Query: 212 EYSRDKARDKLKRVRYFQHSSAGFLVAITASFTV----IVVTHGIALFVAAPGFL----- 271
              + + R K++  + F     G+ + IT S  V    I+  H I    AAP  L     
Sbjct: 216 TSKKRRIRRKIRFFK-FCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 275

Query: 272 --------VGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMK 331
                    G +  + K   L K   Q+++AAKG + L  D DT+ RL  RL  E+EH K
Sbjct: 276 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 335

Query: 332 VMAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEI 364
            +A   +     K   + E  R+ N   E F+ QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 336 TVAA--MCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 389

BLAST of CSPI05G07300 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-23
Identity = 98/343 (28.57%), Postives = 163/343 (47.52%), Query Frame = 0

Query: 31  DVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLDPPQ 90
           ++  E A+AF+T SY D  +R++ ++       Q +LE           LF+   L P  
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVID-----PTQENLE-----------LFLSQELRPNN 94

Query: 91  PTIKTILT-AHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQNKQEFN 150
            +++  L+  H    + + L+  YF H+ +A+  C  + + +   R  L++   +     
Sbjct: 95  ESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIF 154

Query: 151 HDDSH------FKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRDKA 210
             DSH      F  L   +F    D+  N F        Q  Q    +L  +L+    + 
Sbjct: 155 PRDSHSAIDESFCNLAFDVF-LKLDTFENPFASPESHSFQDTQLCFYQLADKLD---TRI 214

Query: 211 RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK------S 270
           R    RVR   H++AG  + +  +  V+  +     + A P  LV A  L          
Sbjct: 215 RKSKSRVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFK 274

Query: 271 RKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAID 330
           +K    + QLNVAAKGT+ LN+D DTI RLV+RL   +++ K++ +  L+RG D  + I 
Sbjct: 275 KKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDV-YTIP 334

Query: 331 ELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 361
           E  +QL +SH N   QL+ L +H+   F  +N++R+L++KEIL
Sbjct: 335 EFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEIL 356

BLAST of CSPI05G07300 vs. ExPASy TrEMBL
Match: A0A0A0KN20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.0e-195
Identity = 350/353 (99.15%), Postives = 351/353 (99.43%), Query Frame = 0

Query: 19  GGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 78
           GGQHV NYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY
Sbjct: 4   GGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 63

Query: 79  RLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKL 138
           RLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKL
Sbjct: 64  RLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKL 123

Query: 139 HSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEY 198
           HSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEY
Sbjct: 124 HSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEY 183

Query: 199 SRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKKSR 258
           SRDK RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKKSR
Sbjct: 184 SRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKKSR 243

Query: 259 KLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAIDE 318
           KLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLD+GGDKRWAIDE
Sbjct: 244 KLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKGGDKRWAIDE 303

Query: 319 LARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL 372
           LARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL
Sbjct: 304 LARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL 356

BLAST of CSPI05G07300 vs. ExPASy TrEMBL
Match: A0A1S3ATJ2 (UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 5.6e-193
Identity = 347/371 (93.53%), Postives = 357/371 (96.23%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKKLKIISCL+KFLSCS G QHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN
Sbjct: 1   MKKLKIISCLRKFLSCSDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANA 120
           LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIK +LTAHLGPNSCSLLLDHYFSHT+NA
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLTAHLGPNSCSLLLDHYFSHTSNA 120

Query: 121 SLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCME 180
           SLLCSRILK IVHLRLKL SLDQNKQEFN+DDSHFKQLLV L EFSN+STPNSFVPYCME
Sbjct: 121 SLLCSRILKHIVHLRLKLRSLDQNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPYCME 180

Query: 181 QVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF 240
           QVQIIQNGCSKLLKRLEYSRDKA+DKLKRVRYFQHSSAGFLVAITASFT+IVVTHGIALF
Sbjct: 181 QVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALF 240

Query: 241 VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKV 300
           VAAPGFLVGAIKL KKSRKLAK+VAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHM+V
Sbjct: 241 VAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRV 300

Query: 301 MAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360
           M KFWLDRG DKRWAI EL RQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL
Sbjct: 301 MTKFWLDRGEDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360

Query: 361 NLSKPIKISYL 372
           + S PIKISYL
Sbjct: 361 DSSAPIKISYL 371

BLAST of CSPI05G07300 vs. ExPASy TrEMBL
Match: A0A6J1DZ81 (UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 7.4e-129
Identity = 255/379 (67.28%), Postives = 303/379 (79.95%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           M+K KI+S L KFLSCS G  H+T+YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD 
Sbjct: 1   MRKFKIVSRLIKFLSCSDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD- 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTIL-TAHLGPNSCSLLLDHYFSHTAN 120
           LT Q+ +ESTTATRLSSYRLF EHLLDP QPT+K +L + +L PNS SLL D YFSHTAN
Sbjct: 61  LTTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLK-------LHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPN 180
           ASLLC R+LK I +LRLK       LHS++  +   N+   +F+ +L  L EFSN   P 
Sbjct: 121 ASLLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNY---NFEPILTCLVEFSNAHNPF 180

Query: 181 SFVPYCMEQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIV 240
                   +++IIQ+GCS LLK+LE++RDKAR KL+RVRYFQH SAGFLVA+T S TVIV
Sbjct: 181 VSSAPSTRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIV 240

Query: 241 VTHGIALFVAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLS 300
           +THGIALFVAAPG LV +I+LA KSRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLS
Sbjct: 241 MTHGIALFVAAPGLLVASIELA-KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLS 300

Query: 301 HELEHMKVMAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRAR 360
           HELEHM+VM +FWL+R  D+R AI E+ARQL Q+H +F+QQLDELEEHLYLCFMTINRAR
Sbjct: 301 HELEHMRVMTRFWLERREDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRAR 360

Query: 361 NLVVKEILNLSKPIKISYL 372
           NLVVKEIL+  +P K  ++
Sbjct: 361 NLVVKEILDPGQPAKAPHV 373

BLAST of CSPI05G07300 vs. ExPASy TrEMBL
Match: A0A6J1H2B8 (UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 6.7e-98
Identity = 223/369 (60.43%), Postives = 262/369 (71.00%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKK K++S L+ FL  S GG H+ + P+  DV EEYANAFRTESY+DFWTRVVAL +   
Sbjct: 1   MKKSKLVSRLRTFL--SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD--- 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILT-AHLGPNSCSLLLDHYFSHTAN 120
               V +ESTTA+RLSSYRLF EHLLDP +PT+K IL  AHL PN  SLL D YFSHTAN
Sbjct: 61  ----VVVESTTASRLSSYRLFAEHLLDPTEPTVKRILNWAHLRPNFKSLLSD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCM 180
           ASLLCSR+LK + HLR ++        EFN     FK L          S  N F     
Sbjct: 121 ASLLCSRLLKDMNHLRPEIAIQSLQNPEFN-----FKPL----------SIHNPF----- 180

Query: 181 EQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIAL 240
               IIQNGC+KLLK+LE++RDKAR K+KRVRYFQHSSAG LVA+TAS TVI+VTHGI L
Sbjct: 181 --PAIIQNGCAKLLKQLEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGL 240

Query: 241 FV--AAPGF--LVGAIKLAKKSRKLAKEVAQ-LNVAAKGTYTLNRDFDTIGRLVARLSHE 300
            V  AAPG   LVGA+KLA+      KEVA+ L+VAAK TYTLNRDFDT+GRLVARL+ E
Sbjct: 241 VVVAAAPGLVGLVGAVKLAR-----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQE 300

Query: 301 LEHMKVMAKFWLDRG---GDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRA 360
           +EHMK + +FW++RG     +   + E+ARQL Q   N  Q LDELEEHLYLCFMTINRA
Sbjct: 301 VEHMKGLMRFWVERGEGRDGRHGGVGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRA 332

BLAST of CSPI05G07300 vs. ExPASy TrEMBL
Match: A0A6J1JZN7 (UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.5e-97
Identity = 221/369 (59.89%), Postives = 262/369 (71.00%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKK K++S L+K  S S GG H+ + P+  DV EEYANAFRTESY+DFWTRVVAL +   
Sbjct: 1   MKKSKLVSRLRKLRSSSDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD--- 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILT-AHLGPNSCSLLLDHYFSHTAN 120
               V++ESTTA+RLSSYRLF EHLLDP +PT+K IL  AHL PNS SLL D YFSHTAN
Sbjct: 61  ----VAVESTTASRLSSYRLFAEHLLDPNEPTVKRILNWAHLRPNSKSLLSD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCM 180
           ASLLCSR+LK I HLR ++        EFN     FK L          S  N F     
Sbjct: 121 ASLLCSRLLKDINHLRPEIAIQSLQNPEFN-----FKPL----------SIHNPF----- 180

Query: 181 EQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIAL 240
              +IIQNGC+KLLK+LE++RDKAR K+KRVRYFQH SAG LVA+TAS TVI+VTHGI L
Sbjct: 181 --PEIIQNGCAKLLKQLEFNRDKARTKVKRVRYFQHGSAGLLVAVTASLTVILVTHGIGL 240

Query: 241 FV--AAP--GFLVGAIKLAKKSRKLAKEVAQ-LNVAAKGTYTLNRDFDTIGRLVARLSHE 300
            V  AAP    LVGA+KLA+      KEVA+ L+VAAK TYTLNRDFDT+GRLVARL+ E
Sbjct: 241 VVVAAAPCLAGLVGAVKLAR-----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQE 300

Query: 301 LEHMKVMAKFWLDRG---GDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRA 360
           +EHM+ + +FW++RG     +   + E+ARQL Q   N  Q LDELEE LYLCFMTINRA
Sbjct: 301 VEHMRGLMRFWVERGEGRDGRHGGVGEVARQLQQCRLNLGQHLDELEELLYLCFMTINRA 334

BLAST of CSPI05G07300 vs. NCBI nr
Match: XP_031741820.1 (UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein Csa_000030 [Cucumis sativus])

HSP 1 Score: 724.2 bits (1868), Expect = 5.9e-205
Identity = 367/371 (98.92%), Postives = 368/371 (99.19%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKKLKIISCLKKFLSCS GGQHV NYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN
Sbjct: 1   MKKLKIISCLKKFLSCSDGGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANA 120
           LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANA
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANA 120

Query: 121 SLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCME 180
           SLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCME
Sbjct: 121 SLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCME 180

Query: 181 QVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF 240
           QVQIIQNGCSKLLKRLEYSRDK RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF
Sbjct: 181 QVQIIQNGCSKLLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF 240

Query: 241 VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKV 300
           VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKV
Sbjct: 241 VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKV 300

Query: 301 MAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360
           MAKFWLD+GGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL
Sbjct: 301 MAKFWLDKGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360

Query: 361 NLSKPIKISYL 372
           NLSKPIKISYL
Sbjct: 361 NLSKPIKISYL 371

BLAST of CSPI05G07300 vs. NCBI nr
Match: XP_008436996.1 (PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo])

HSP 1 Score: 683.3 bits (1762), Expect = 1.1e-192
Identity = 347/371 (93.53%), Postives = 357/371 (96.23%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKKLKIISCL+KFLSCS G QHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN
Sbjct: 1   MKKLKIISCLRKFLSCSDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANA 120
           LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIK +LTAHLGPNSCSLLLDHYFSHT+NA
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLTAHLGPNSCSLLLDHYFSHTSNA 120

Query: 121 SLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCME 180
           SLLCSRILK IVHLRLKL SLDQNKQEFN+DDSHFKQLLV L EFSN+STPNSFVPYCME
Sbjct: 121 SLLCSRILKHIVHLRLKLRSLDQNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPYCME 180

Query: 181 QVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF 240
           QVQIIQNGCSKLLKRLEYSRDKA+DKLKRVRYFQHSSAGFLVAITASFT+IVVTHGIALF
Sbjct: 181 QVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALF 240

Query: 241 VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKV 300
           VAAPGFLVGAIKL KKSRKLAK+VAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHM+V
Sbjct: 241 VAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRV 300

Query: 301 MAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360
           M KFWLDRG DKRWAI EL RQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL
Sbjct: 301 MTKFWLDRGEDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 360

Query: 361 NLSKPIKISYL 372
           + S PIKISYL
Sbjct: 361 DSSAPIKISYL 371

BLAST of CSPI05G07300 vs. NCBI nr
Match: XP_038875828.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.5e-155
Identity = 297/378 (78.57%), Postives = 331/378 (87.57%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKK KI+SCL+KFLSCS G  HVT++ VD DV EEYANAFRTESYIDFWTRV+ALNNGD+
Sbjct: 1   MKKSKIVSCLRKFLSCSDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDD 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILTA-HLGPNSCSLLLDHYFSHTAN 120
           LT Q+S+ESTTAT LSSYRLFVEHLLDPPQ TIK ILT+ HLG NSCSLLLD YFSHTAN
Sbjct: 61  LTTQLSVESTTATHLSSYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLKLHSLD------QNKQEFNHDDSHFKQLLVRLFEFSNDSTPNS 180
           ASL CS +LK I HLRLKLH L+      +NK++F+H +SHFKQLL+RL EFSN  T N 
Sbjct: 121 ASLSCSHLLKHINHLRLKLHPLEITLQSLENKEKFHH-ESHFKQLLIRLVEFSN--TRNP 180

Query: 181 FVPYCMEQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVV 240
           F+P  +EQVQIIQNGCSKLLK+LE+SRDKA+ KLKRVRYFQHSSAGFLVA+TAS TVI+V
Sbjct: 181 FIP-SIEQVQIIQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIV 240

Query: 241 THGIALFVAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSH 300
           THGIALFVAAPGFLVGAIKLA + RKL K+VA+LN+ AKGTYTLNRDFDTIGRLVARL+H
Sbjct: 241 THGIALFVAAPGFLVGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNH 300

Query: 301 ELEHMKVMAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARN 360
           ELEHM+VMA+FWLDRG DKR AI EL RQLNQSH NF+ QLDELEEHLYLCFMTINRARN
Sbjct: 301 ELEHMRVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARN 360

Query: 361 LVVKEILNLSKPIKISYL 372
           LVVKEIL+  +PIKISYL
Sbjct: 361 LVVKEILDSGQPIKISYL 373

BLAST of CSPI05G07300 vs. NCBI nr
Match: XP_022159588.1 (UPF0496 protein At3g49070 [Momordica charantia])

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-128
Identity = 255/379 (67.28%), Postives = 303/379 (79.95%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           M+K KI+S L KFLSCS G  H+T+YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD 
Sbjct: 1   MRKFKIVSRLIKFLSCSDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD- 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTIL-TAHLGPNSCSLLLDHYFSHTAN 120
           LT Q+ +ESTTATRLSSYRLF EHLLDP QPT+K +L + +L PNS SLL D YFSHTAN
Sbjct: 61  LTTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLK-------LHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPN 180
           ASLLC R+LK I +LRLK       LHS++  +   N+   +F+ +L  L EFSN   P 
Sbjct: 121 ASLLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNY---NFEPILTCLVEFSNAHNPF 180

Query: 181 SFVPYCMEQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIV 240
                   +++IIQ+GCS LLK+LE++RDKAR KL+RVRYFQH SAGFLVA+T S TVIV
Sbjct: 181 VSSAPSTRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIV 240

Query: 241 VTHGIALFVAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLS 300
           +THGIALFVAAPG LV +I+LA KSRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLS
Sbjct: 241 MTHGIALFVAAPGLLVASIELA-KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLS 300

Query: 301 HELEHMKVMAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRAR 360
           HELEHM+VM +FWL+R  D+R AI E+ARQL Q+H +F+QQLDELEEHLYLCFMTINRAR
Sbjct: 301 HELEHMRVMTRFWLERREDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRAR 360

Query: 361 NLVVKEILNLSKPIKISYL 372
           NLVVKEIL+  +P K  ++
Sbjct: 361 NLVVKEILDPGQPAKAPHV 373

BLAST of CSPI05G07300 vs. NCBI nr
Match: KAG6605733.1 (UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.0 bits (946), Expect = 4.8e-98
Identity = 224/369 (60.70%), Postives = 262/369 (71.00%), Query Frame = 0

Query: 1   MKKLKIISCLKKFLSCSGGGQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60
           MKK K++S L+KFL  S GG H+ + P+  DV EEYANAFRTESY+DFWTRVVAL +   
Sbjct: 1   MKKSKLVSRLRKFL--SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD--- 60

Query: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILT-AHLGPNSCSLLLDHYFSHTAN 120
               V +ESTTA+RLSSYRLF EHLLDP +PT+K IL  AHL PN  SLL D YFSHTAN
Sbjct: 61  ----VVVESTTASRLSSYRLFAEHLLDPTEPTVKRILNWAHLRPNFKSLLSD-YFSHTAN 120

Query: 121 ASLLCSRILKQIVHLRLKLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCM 180
           ASLLCSR+LK I HLR ++        EFN     FK L          S  N F     
Sbjct: 121 ASLLCSRLLKDINHLRPEIAIQSLQNPEFN-----FKPL----------SIHNPF----- 180

Query: 181 EQVQIIQNGCSKLLKRLEYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIAL 240
               IIQNGC+KLLK+LE++RDKAR K+KRVRYFQHSSAG LVA+TAS TVI+VTHGI L
Sbjct: 181 --PAIIQNGCAKLLKQLEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGL 240

Query: 241 FV--AAPGF--LVGAIKLAKKSRKLAKEVAQ-LNVAAKGTYTLNRDFDTIGRLVARLSHE 300
            V  AAPG   LVGA+KLA+      KEV + L+VAAK TYTLNRDFDT+GRLVARL+ E
Sbjct: 241 VVVAAAPGLAGLVGAVKLAR-----VKEVTRVLDVAAKATYTLNRDFDTVGRLVARLNQE 300

Query: 301 LEHMKVMAKFWLDRG---GDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRA 360
           +EHMK + +FW++RG     +   + E+ARQL Q   N  Q LDELEEHLYLCFMTINRA
Sbjct: 301 VEHMKGLMRFWVERGEGRDGRHGGLGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRA 332

BLAST of CSPI05G07300 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 275.0 bits (702), Expect = 8.8e-74
Identity = 163/347 (46.97%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 29  DTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSL-----ESTTATRLSSYRLFVE 88
           D DV EEYANAFRTESY  FWTRVV L+   +  +  S       S+T+ RL SYRLF  
Sbjct: 68  DVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH 127

Query: 89  HLLDPPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ 148
           +LLDP   TI  IL           LL  YF  TANA LLC+++LK I HLR K  SL  
Sbjct: 128 NLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESL-- 187

Query: 149 NKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRDKA 208
            K +F+ ++ +   L+ +  E S    P  F+     ++Q+I++GC  LLKRLE  RDK 
Sbjct: 188 -KPKFHSENHNSLALIDQFTEISKWFDP--FIS-SGSRIQLIRSGCLYLLKRLESRRDKT 247

Query: 209 RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKKSRKLAKE 268
           R KLK +    HSS   ++A+T +  V + +H  ALF+AAP  L    K A    KL K 
Sbjct: 248 RAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKLTKT 307

Query: 269 VAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAIDELARQL 328
            A+L+VAAKGTY L+RD DTI RLV R++ E+ H++ MA+FW+ RG  +    +E+AR+L
Sbjct: 308 AARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVAREL 367

Query: 329 NQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISY 371
            +  E+F+++LDELEEH+YLCFMTINRARNL+VKEIL+   P   S+
Sbjct: 368 KRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDPPNCSF 408

BLAST of CSPI05G07300 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 115.5 bits (288), Expect = 8.9e-26
Identity = 109/364 (29.95%), Postives = 174/364 (47.80%), Query Frame = 0

Query: 32  VGEEYANAFRTESYIDFWTRV---VALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLDP 91
           V EEY  AFRT SY++  T+    + + +   L++  S   ++++ LS +  F ++LLDP
Sbjct: 36  VNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLLDP 95

Query: 92  PQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ----N 151
           PQ T    L A +  +S   L+  +F  ++ A  +C  +L+ +  +++  + + +     
Sbjct: 96  PQET----LDALMQDSSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKIG 155

Query: 152 KQEFNHD---DSHFKQLLVRLF-EFSNDSTPNSFVPYCM----EQVQIIQNGCSKLLKRL 211
           K+  N     +   + L   +F E S  +   +  P C      Q +I+ +  S LL +L
Sbjct: 156 KRVCNGAKTLECSPEMLCALIFQELSRFAALKN--PLCRIVNEAQFRIVHDANSDLLTKL 215

Query: 212 EYSRDKARDKLKRVRYFQHSSAGFLVAITASFTV----IVVTHGIALFVAAPGFL----- 271
              + + R K++  + F     G+ + IT S  V    I+  H I    AAP  L     
Sbjct: 216 TSKKRRIRRKIRFFK-FCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 275

Query: 272 --------VGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMK 331
                    G +  + K   L K   Q+++AAKG + L  D DT+ RL  RL  E+EH K
Sbjct: 276 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 335

Query: 332 VMAKFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEI 364
            +A   +     K   + E  R+ N   E F+ QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 336 TVAA--MCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 389

BLAST of CSPI05G07300 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 112.5 bits (280), Expect = 7.5e-25
Identity = 98/343 (28.57%), Postives = 163/343 (47.52%), Query Frame = 0

Query: 31  DVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLDPPQ 90
           ++  E A+AF+T SY D  +R++ ++       Q +LE           LF+   L P  
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVID-----PTQENLE-----------LFLSQELRPNN 94

Query: 91  PTIKTILT-AHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQNKQEFN 150
            +++  L+  H    + + L+  YF H+ +A+  C  + + +   R  L++   +     
Sbjct: 95  ESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIF 154

Query: 151 HDDSH------FKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRLEYSRDKA 210
             DSH      F  L   +F    D+  N F        Q  Q    +L  +L+    + 
Sbjct: 155 PRDSHSAIDESFCNLAFDVF-LKLDTFENPFASPESHSFQDTQLCFYQLADKLD---TRI 214

Query: 211 RDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK------S 270
           R    RVR   H++AG  + +  +  V+  +     + A P  LV A  L          
Sbjct: 215 RKSKSRVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFK 274

Query: 271 RKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAID 330
           +K    + QLNVAAKGT+ LN+D DTI RLV+RL   +++ K++ +  L+RG D  + I 
Sbjct: 275 KKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDV-YTIP 334

Query: 331 ELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEIL 361
           E  +QL +SH N   QL+ L +H+   F  +N++R+L++KEIL
Sbjct: 335 EFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEIL 356

BLAST of CSPI05G07300 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-23
Identity = 100/363 (27.55%), Postives = 173/363 (47.66%), Query Frame = 0

Query: 31  DVGEEYANAFRTESYIDFWTR---VVALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLD 90
           ++  E A+AF+T SY D  +R   VV L    +   Q  +E           L +  +L 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIE-----------LLLSQVLQ 105

Query: 91  PPQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLH-------- 150
           P +  ++  +  H+   + + L+  YF H+ +A+ LC  + + +   R  L+        
Sbjct: 106 PNKECVQEAI-RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFN 165

Query: 151 --------SLDQNKQEFNHDDSHFKQLLVRLFEFSND-STPNSFVPYCMEQVQIIQNGCS 210
                   ++D++  +   D      + ++L  F N  S+P S   Y     Q+      
Sbjct: 166 IFPGDSLPAIDESLCDLAFD------VFLKLDTFENPFSSPES---YSFRDTQL------ 225

Query: 211 KLLKRLEYSRD-KARDKLKRVRYFQHSSAG-------FLVAITASFTVIVVTHGIALFVA 270
               +L+++ D + R    RVR   H++AG        +VA+ AS  V++ +H + + + 
Sbjct: 226 -CFSQLKHNLDRRLRKSRSRVRLIHHATAGSSLCLVAAVVAVAAS-AVVIASHALPILLV 285

Query: 271 APGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMA 330
             G L          RK    + QLN A+KGT+ LN+D DTI RLV+RL   +E+ K + 
Sbjct: 286 VAGPLCSPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLI 345

Query: 331 KFWLDRGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNL 366
           +  L+RG D   +I E+ + L +SH     QL +LE+H+ L F  +N+AR+L++ EI +L
Sbjct: 346 RLGLERGRDVH-SIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI-HL 377

BLAST of CSPI05G07300 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 104.4 bits (259), Expect = 2.0e-22
Identity = 101/347 (29.11%), Postives = 167/347 (48.13%), Query Frame = 0

Query: 32  VGEEYANAFRTESYIDFWTRV---VALNNGDNLTAQVSLESTTATRLSSYRLFVEHLLDP 91
           V EEY  AFRT SY++  T+    + + +   L++  S   ++++ LS +  F ++LLDP
Sbjct: 36  VNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLLDP 95

Query: 92  PQPTIKTILTAHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLKLHSLDQ----N 151
           PQ T    L A +  +S   L+  +F  ++ A  +C  +L+ +  +++  + + +     
Sbjct: 96  PQET----LDALMQDSSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKIG 155

Query: 152 KQEFNHD---DSHFKQLLVRLF-EFSNDSTPNSFVPYCM----EQVQIIQNGCSKLLKRL 211
           K+  N     +   + L   +F E S  +   +  P C      Q +I+ +  S LL +L
Sbjct: 156 KRVCNGAKTLECSPEMLCALIFQELSRFAALKN--PLCRIVNEAQFRIVHDANSDLLTKL 215

Query: 212 EYSRDKARDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK 271
              + + R K+  +  F   +A  L+ +  SF ++                 G +  + K
Sbjct: 216 TSKKRRIRRKISILGVF---AAPALLGL-CSFCLLRKKKA-----------KGRMHKSNK 275

Query: 272 SRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDRGGDKRWAI 331
              L K   Q+++AAKG + L  D DT+ RL  RL  E+EH K +A   +     K   +
Sbjct: 276 DTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAA--MCAKSRKIEVL 335

Query: 332 DELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLS 364
            E  R+ N   E F+ QL ELEEHLYLCF TINR+R LV+ +I   S
Sbjct: 336 KEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQITGQS 358