CSPI05G06970 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G06970 vs. ExPASy Swiss-Prot
Match: A0A1P8AUY4 (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 3.7e-251
Identity = 438/699 (62.66%), Postives = 546/699 (78.11%), Query Frame = 0

Query: 24   YLKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGC 83
            +L   LE    DTL    +L R++ YGWG+ +     S   S++   +FGIDPFFI KG 
Sbjct: 1695 FLLQQLELFASDTLP--LELSRMELYGWGDSKAICEKSK--SVRHEGMFGIDPFFISKGD 1754

Query: 84   QLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRI 143
            +  + G +EF+APTT++N LRVLRAMQLSKP+LLEGSPGVGKTSLI+ALG++SGHKVVRI
Sbjct: 1755 ENPEIGGFEFLAPTTHRNVLRVLRAMQLSKPILLEGSPGVGKTSLILALGKYSGHKVVRI 1814

Query: 144  NLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLN 203
            NLSEQTDMMDLLGSDLPVESDE +KFAWSDGILLQAL+EG WVLLDELNLAPQSVLEGLN
Sbjct: 1815 NLSEQTDMMDLLGSDLPVESDEDMKFAWSDGILLQALKEGSWVLLDELNLAPQSVLEGLN 1874

Query: 204  AILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIE 263
            AILDHRA+VFIPEL  TF+CPP+FRVFACQNPS QGGGRKGLPKSFLNRFTKVY+DEL+E
Sbjct: 1875 AILDHRAQVFIPELGCTFECPPTFRVFACQNPSTQGGGRKGLPKSFLNRFTKVYVDELVE 1934

Query: 264  DDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQII 323
            DDYLFIC SLY S+P PLLSKLI  N++LH+  ML++KF  DGSPWEFNLRDV+RSCQ +
Sbjct: 1935 DDYLFICRSLYPSVPSPLLSKLIALNRQLHDGTMLYRKFGHDGSPWEFNLRDVIRSCQFM 1994

Query: 324  EGAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVG 383
            + A   L    FLN++Y+QRMRTA DR+EVLR+Y+ +F     INPYPRVQLN  +L+VG
Sbjct: 1995 QEAIHDLEVESFLNVLYIQRMRTATDRKEVLRIYKAIFDKTPSINPYPRVQLNPAYLVVG 2054

Query: 384  NIAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLA 443
              AI RN  Q+ N+AS QLK+LP IRQ+LEAVAHC+Q +W+CILVGPSSSGKTS++R+LA
Sbjct: 2055 TAAIKRNLNQS-NIASEQLKLLPEIRQNLEAVAHCVQNKWLCILVGPSSSGKTSVIRILA 2114

Query: 444  QLTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEF 503
            QLTG  LNELNLSS TD S+LLGCFEQY+A RNF  V+ +V   V++Y S+ ++ S++  
Sbjct: 2115 QLTGYPLNELNLSSATDSSDLLGCFEQYNAFRNFRLVMTRVEHLVDEYNSLLLQSSQEAL 2174

Query: 504  DRDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQE--VP 563
              + + ++++WLS+ +KI   L  +   +  + +    +L  L ++++ L   ++E  +P
Sbjct: 2175 FSNRSGLVSRWLSYLNKIDSSLVENPLFFLNDSE----TLSTLEEVVEDLEQVLKEGVLP 2234

Query: 564  ---AKKELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTV 623
               +KK LE+  KT+L+L+   +K   S KFEWV G+L+KAIE+GEW++LKNAN CNPTV
Sbjct: 2235 VSWSKKYLEQISKTILQLQTHEKKQ--STKFEWVTGMLIKAIEKGEWVVLKNANLCNPTV 2294

Query: 624  LDRINSLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIF 683
            LDRINSLVE CGSITINECG ++GEPV + PH NFR+FL+VNP  GEVSRAMRNRGVE+F
Sbjct: 2295 LDRINSLVEPCGSITINECGIVNGEPVTVVPHPNFRLFLSVNPKFGEVSRAMRNRGVEVF 2354

Query: 684  MLQPHWL--QDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            M+ PHW   +DG+ C  +++ L    RFLALSGIPG KL
Sbjct: 2355 MMGPHWQLNEDGSNC--EELVLRGVERFLALSGIPGYKL 2380

BLAST of CSPI05G06970 vs. ExPASy Swiss-Prot
Match: Q869L3 (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2)

HSP 1 Score: 528.5 bits (1360), Expect = 1.2e-148
Identity = 294/703 (41.82%), Postives = 424/703 (60.31%), Query Frame = 0

Query: 18   SLSLGVYLKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNT--DSMQDSNLFGID 77
            S S G+ L+     RL D +    + ++LQ+       ++S+ S++  + +++ N FGI 
Sbjct: 1749 SESEGLKLREACLIRLIDQIESQDEKLKLQTELLSTSSSSSSLSSSIANIIKEENRFGIH 1808

Query: 78   PFFIEKGCQLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQF 137
            PF+I          ++   APTT KNA RVLR MQL + +L+EGSPGVGKTSLI A+   
Sbjct: 1809 PFYIPVRESNVPKIQFSLSAPTTSKNAQRVLRGMQLPRAILVEGSPGVGKTSLITAIANA 1868

Query: 138  SGHKVVRINLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAP 197
            SG+++VRINLSEQTD+MDLLGSDLPVE   G +F W DG+ L+ALR G WVLLDELNLA 
Sbjct: 1869 SGNQIVRINLSEQTDIMDLLGSDLPVEGGTGGQFEWRDGVFLEALRNGSWVLLDELNLAS 1928

Query: 198  QSVLEGLNAILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTK 257
            Q+VLEGLN+ LDHR+EV+IPEL  TF C P+FRVFACQNP +QGGGRKGLPKSFLNRFT+
Sbjct: 1929 QTVLEGLNSCLDHRSEVYIPELGKTFACHPNFRVFACQNPLHQGGGRKGLPKSFLNRFTQ 1988

Query: 258  VYLDELIEDDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRD 317
            V++D+L + D LFI +++Y +IP   + ++I FN ++ +E M+  KFA+ G+PWEFNLRD
Sbjct: 1989 VFIDQLNQQDLLFISTAMYPTIPSKTIERMIEFNHQMFKESMIEHKFARKGAPWEFNLRD 2048

Query: 318  VLRSCQIIEGAPERLRSYC-FLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLIN------ 377
              R C +I   P  + +   F++++Y+QRMRT  DR  VL +++ VF      N      
Sbjct: 2049 TFRWCDLIVKDPTSISNPARFIDLIYLQRMRTLQDREHVLTIFKRVFTTSGDENDQSIDM 2108

Query: 378  --------PYPRVQLNSRFLIVGNIAI------GRNSIQACNV-ASSQLKILPGIRQSLE 437
                      P   ++  +L +G+  +      G +S  +  +  SS +++L  +   LE
Sbjct: 2109 MPTICNYDKQPHYSISPEYLQIGSSILPRVQNGGASSFDSSVLDNSSNIQLLQRLLNPLE 2168

Query: 438  AVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLTGNVLNELNLSSTTDISELLGCFEQYDA 497
             +  CI+  WM IL+GP+S+ KTS +RLLAQLTGN L E +++S+ D +E+LG FEQ D 
Sbjct: 2169 NIMKCIEMNWMSILIGPTSTSKTSSIRLLAQLTGNTLYEFSMNSSVDTTEILGGFEQIDL 2228

Query: 498  IRNFHHVIDQ-------VGFHVNKYCSVQIRCSKKEFDRDGNCIMTKWLSF---SSKISF 557
            +R    +I         V  H+  Y +       K        I   W  F   + +   
Sbjct: 2229 VRYQKRIISSVSSLITIVSSHIMTYFNSNDDNLMKTCISSIQDIHQVWNLFKKQTQQTQQ 2288

Query: 558  QLPSSACVYAKNWKRIVC-----SLGLLVDIIKQLMSFVQEVPAKKELERC--LKTVLKL 617
            Q+             ++       L  +VD +++L         ++ L R   ++  ++ 
Sbjct: 2289 QIQQDGATIGSGGVGLITIEQLDLLSTIVDALEKLSIQFNLDNNQEHLNRIQDVRQQIQR 2348

Query: 618  EESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLVESCGSITINE 677
             +S +K   +  FEW+ G+L+KA+E G WI++ N N CNPTVLDR+N L+E  G + +NE
Sbjct: 2349 IKSIEKESVTGCFEWIDGLLIKALETGAWILIDNVNFCNPTVLDRLNPLLEQDGVLMLNE 2408

Query: 678  CGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFM 680
             G +DG+  V+ PH NFRIFLT++   GE+SRAMRNRG+EI+M
Sbjct: 2409 RGMVDGQVKVVKPHPNFRIFLTMDDKKGEISRAMRNRGIEIYM 2451

BLAST of CSPI05G06970 vs. ExPASy Swiss-Prot
Match: Q9NU22 (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 1.6e-137
Identity = 276/649 (42.53%), Postives = 401/649 (61.79%), Query Frame = 0

Query: 41   SKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQL--GDTGKYEFMAPTT 100
            +K+VRL  Y   EL+        +     NL+GI PFFI +G  L   +   Y   A TT
Sbjct: 1673 AKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGPVLHRNNIADYALSAGTT 1732

Query: 101  YKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLSEQTDMMDLLGSD 160
              NA R+LRA +L KP+LLEGSPGVGKTSL+ AL + SG+ +VRINLSEQTD+ DL G+D
Sbjct: 1733 AMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGAD 1792

Query: 161  LPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAILDHRAEVFIPELA 220
            LPVE  +G +FAW DG LL AL+ G WV+LDELNLA QSVLEGLNA  DHR E+++PEL 
Sbjct: 1793 LPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELG 1852

Query: 221  LTFKCP-PSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDYLFICSSLYESI 280
            ++F+      ++F CQNP  QGGGRKGLP+SFLNRFT+V++D L   D  FI S+L+ +I
Sbjct: 1853 MSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAI 1912

Query: 281  PKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQ--IIEGAPERLRSYCF 340
             K ++ K++ FN ++  EV + KK+ Q G PWEFNLRD+ R CQ  +++ +P        
Sbjct: 1913 EKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQSPGCYDPGQH 1972

Query: 341  LNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQL---NSRFLIVGNIAIGRNSI 400
            + +VY +RMRT  D+++V+ ++++VFG+    NPY   +L       + +G   + R S 
Sbjct: 1973 VFLVYGERMRTEEDKKKVIAVFKDVFGSN--SNPYMGTRLFRITPYDVQLGYSVLSRGSC 2032

Query: 401  QACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLTGNVLNE 460
               + +   L +L    Q LE++  C+Q  WM ILVGP+S GKTSLV+LLA LTG+ L  
Sbjct: 2033 -VPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKI 2092

Query: 461  LNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRDGNCIMT 520
            + ++S  D +ELLG FEQ D IR +  ++++V   V       +  S      D   ++ 
Sbjct: 2093 MAMNSAMDTTELLGGFEQVDLIRPWRRLLEKVEGTVRALLRDSLLISAD----DAEVVLR 2152

Query: 521  KWLSF--SSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKELERCL 580
             W  F  + K          +  +   ++   L L+  +  ++ S+ +   AK  +E   
Sbjct: 2153 AWSHFLLTYKPKCLGEGGKAITMEIVNKLEAVLLLMQRLNNKINSYCKAEFAKL-VEEFR 2212

Query: 581  KTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLVESCG 640
               +KL +    H     FEWV  +LV+A++ G+W+++ N N CNP+VLDR+N+L+E  G
Sbjct: 2213 SFGVKLTQLASGHSHGT-FEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGG 2272

Query: 641  SITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFM 680
             +TI+E G IDG    + P+ NFR+FL+++P+HG++SRAMRNRG+EI++
Sbjct: 2273 VLTISERGMIDGSTPTITPNPNFRLFLSMDPVHGDISRAMRNRGLEIYI 2312

BLAST of CSPI05G06970 vs. ExPASy Swiss-Prot
Match: O94248 (Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.3e-131
Identity = 289/686 (42.13%), Postives = 388/686 (56.56%), Query Frame = 0

Query: 7    FLPFQGDHQTCSLSLGVYLKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSM 66
            F+   G   T SLS  +   H   QR    L+E +                SN   + S 
Sbjct: 1464 FIDALGSFTTFSLSNNLASLHAERQRCFAKLNELA---------------GSNIMASKSA 1523

Query: 67   QDSNLFGIDPFFI-EKGCQLGDT----GKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSP 126
              S  F    FFI + G  LGD+      Y     TT  NA +VLRA+Q+ KP+LLEGSP
Sbjct: 1524 DISIKFSDSSFFIGDFGIPLGDSVESDSTYSLHTDTTLMNASKVLRALQVLKPILLEGSP 1583

Query: 127  GVGKTSLIVALGQFSGHKVVRINLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALR 186
            GVGKTSLI AL + +GH++VRINLS+QTD+MDL GSD+PVE  EG +FAW D   L A+R
Sbjct: 1584 GVGKTSLITALARETGHQLVRINLSDQTDLMDLFGSDVPVEGGEGGQFAWRDAPFLAAMR 1643

Query: 187  EGCWVLLDELNLAPQSVLEGLNAILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGG 246
             G WVLLDELNLA QSVLEGLNA LDHR E +IPEL   FK  P+FRVFA QNP +QGGG
Sbjct: 1644 NGHWVLLDELNLASQSVLEGLNACLDHRNEAYIPELDKVFKAHPNFRVFAAQNPQHQGGG 1703

Query: 247  RKGLPKSFLNRFTKVYLDELIEDDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKK 306
            RKGLP+SF+NRF+ VY++ L E D + I +  Y  + +    K+I F  RL + +     
Sbjct: 1704 RKGLPRSFINRFSVVYVEALKEKDMIEIAACNYHQVNEDWRLKIIKFMFRLQDNIEKDIS 1763

Query: 307  FAQDGSPWEFNLRDVLRSCQIIEGAPER--LRSYCFLNIVYVQRMRTAGDRREVLRLYEE 366
            F   GSPWEFNLRD LR  Q++  AP+   +    +L ++ + RMRT  DR     L++E
Sbjct: 1764 FGSFGSPWEFNLRDTLRWLQLLNDAPKYTCVSPADYLEVMVLHRMRTVEDRVRTCELFKE 1823

Query: 367  VFGAKYLINPYPRV---QLNSRFLIVGNIAIGRNSIQACNVASSQLKILPGIRQSLEAVA 426
            VF   Y     PR     L+S+   VG+  + R+  +   +  SQ  IL      LE+V 
Sbjct: 1824 VFDIDY----EPRTIGFSLSSQCFKVGHSLLVRDVERQKTLLDSQ-NILQSQLPVLESVI 1883

Query: 427  HCIQYQWMCILVGPSSSGKTSLVRLLAQLTGNVLNELNLSSTTDISELLGCFEQYDAIRN 486
             CI  +W CILVG +++GKT ++RLLA + G  + E+ ++S TD  +L+G +EQ D  R 
Sbjct: 1884 TCINKKWPCILVGDTATGKTCILRLLAAIAGAKIKEMAVNSDTDTMDLIGEYEQIDISRK 1943

Query: 487  FHHVIDQVGFHVNKYCSVQIRCSKKEFDRDGNCIMTKWLSFSSKISFQLPSSACVYAKNW 546
               +   +   +     + I    + FD   N      L   +  SF+  S A       
Sbjct: 1944 ASELFTDLSQQL-----LNIVIKYRNFD---NIFRETSLYTLTTTSFKTHSQAFTL---- 2003

Query: 547  KRIVCSLGLLVDIIKQLMSFVQEVPAKKEL-ERCLKTVLKLEESNQKHPFSAKFEWVIGI 606
                  L  +VD + QL      V +  ++ E+  K + +   S      + +FEW  G 
Sbjct: 2004 ------LQKVVDQLDQLKIHETLVHSLGDIHEKARKLLAEFSASP-----AGRFEWFDGY 2063

Query: 607  LVKAIERGEWIILKNANSCNPTVLDRINSLVESCGSITINECGTIDGEPVVLHPHANFRI 666
            L+KA+E G W +L NAN C+P VLDR+NSL+E  G + +NE  T DG P  + PH NFR+
Sbjct: 2064 LLKAVEEGHWFVLDNANLCSPAVLDRLNSLLEHKGVLIVNEKTTEDGHPKTIKPHPNFRL 2106

Query: 667  FLTVNPIHGEVSRAMRNRGVEIFMLQ 682
            FLTVNP++GE+SRAMRNRGVEIF+L+
Sbjct: 2124 FLTVNPVYGELSRAMRNRGVEIFLLK 2106

BLAST of CSPI05G06970 vs. ExPASy Swiss-Prot
Match: Q12019 (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 7.0e-125
Identity = 249/600 (41.50%), Postives = 352/600 (58.67%), Query Frame = 0

Query: 88   TGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLSE 147
            +  +   APTT  N +RV+RAMQ+ KP+LLEGSPGVGKTSLI AL   +G+K+ RINLSE
Sbjct: 1716 SSSFNLTAPTTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSE 1775

Query: 148  QTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAILD 207
            QTD++DL G+D P E     +F W D   L+A+++G WVLLDE+NLA QSVLEGLNA LD
Sbjct: 1776 QTDLVDLFGADAPGERSG--EFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLD 1835

Query: 208  HRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDYL 267
            HR E +IPEL ++F C P+F VFA QNP YQGGGRKGLPKSF+NRF+ V++D L  DD L
Sbjct: 1836 HRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLL 1895

Query: 268  FICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE--G 327
             I   LY SI   +++K+I     L ++V   K +   GSPWEFNLRD LR  +++    
Sbjct: 1896 LIAKHLYPSIEPDIIAKMIKLMSTLEDQVCKRKLWGNSGSPWEFNLRDTLRWLKLLNQYS 1955

Query: 328  APERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNI 387
              E +  + F++I+  QR RT  D+ +   L E++FG       +   +L   ++ + N 
Sbjct: 1956 ICEDVDVFDFVDIIVKQRFRTISDKNKAQLLIEDIFGKFSTKENF--FKLTEDYVQINNE 2015

Query: 388  AIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQL 447
               RN      +  +   +   +    E+V   I   W  +LVGPS+SGKT  +R LA +
Sbjct: 2016 VALRNPHYRYPITQNLFPLECNV-AVYESVLKAINNNWPLVLVGPSNSGKTETIRFLASI 2075

Query: 448  TGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDR 507
             G  ++  +++S  D  ++LG +EQ D  R   ++ +++   V +  S+ ++ S      
Sbjct: 2076 LGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIVREIISMNMKLSPNATAI 2135

Query: 508  DGNCIMTKWL--SFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAK 567
                 + K+L  +  +   FQ                       D   +   F   +   
Sbjct: 2136 MEGLNLLKYLLNNIVTPEKFQ-----------------------DFRNRFNRFFSHLEGH 2195

Query: 568  KELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRIN 627
              L+     + K+ E   K   S KFEW  G+LVKA+E+G W+IL NAN C+P+VLDR+N
Sbjct: 2196 PLLKTMSMNIEKMTEIITKEA-SVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLN 2255

Query: 628  SLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPH 684
            SL+E  GS+ INEC   DG+P VL PH NFR+FLT++P +GE+SRAMRNRGVEI++ + H
Sbjct: 2256 SLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDPKYGELSRAMRNRGVEIYIDELH 2286

BLAST of CSPI05G06970 vs. ExPASy TrEMBL
Match: A0A0A0KCN0 (Midasin OS=Cucumis sativus OX=3659 GN=Csa_7G450640 PE=3 SV=1)

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 687/699 (98.28%), Postives = 690/699 (98.71%), Query Frame = 0

Query: 18   SLSLGVY-LKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDP 77
            + S GV+     L  +LDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDP
Sbjct: 1352 AFSCGVFSYNQNLVLQLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDP 1411

Query: 78   FFIEKGCQLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFS 137
            FFIEKGCQLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFS
Sbjct: 1412 FFIEKGCQLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFS 1471

Query: 138  GHKVVRINLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQ 197
            GHKVVRINLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQ
Sbjct: 1472 GHKVVRINLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQ 1531

Query: 198  SVLEGLNAILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKV 257
            SVLEGLNAILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKV
Sbjct: 1532 SVLEGLNAILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKV 1591

Query: 258  YLDELIEDDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDV 317
            YLDELIEDDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDV
Sbjct: 1592 YLDELIEDDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDV 1651

Query: 318  LRSCQIIEGAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLN 377
            LRSCQIIEGAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLN
Sbjct: 1652 LRSCQIIEGAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLN 1711

Query: 378  SRFLIVGNIAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKT 437
            SRFLIVGNIAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKT
Sbjct: 1712 SRFLIVGNIAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKT 1771

Query: 438  SLVRLLAQLTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQI 497
            SLVRLLAQLTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQI
Sbjct: 1772 SLVRLLAQLTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQI 1831

Query: 498  RCSKKEFDRDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSF 557
            RCSKKEFDRDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSF
Sbjct: 1832 RCSKKEFDRDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSF 1891

Query: 558  VQEVPAKKELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNP 617
            VQEVPAKKELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNP
Sbjct: 1892 VQEVPAKKELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNP 1951

Query: 618  TVLDRINSLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVE 677
            TVLDRINSLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVE
Sbjct: 1952 TVLDRINSLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVE 2011

Query: 678  IFMLQPHWLQDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            IFMLQPHWLQDGALCGKKDIELNDTRRFLALSGIPGAKL
Sbjct: 2012 IFMLQPHWLQDGALCGKKDIELNDTRRFLALSGIPGAKL 2050

BLAST of CSPI05G06970 vs. ExPASy TrEMBL
Match: A0A1S3CGN9 (Midasin OS=Cucumis melo OX=3656 GN=LOC103500710 PE=3 SV=1)

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 671/689 (97.39%), Postives = 678/689 (98.40%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQDSNLFGIDPFFIEKGCQLG
Sbjct: 1715 LLEKLKLDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDSNLFGIDPFFIEKGCQLG 1774

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS
Sbjct: 1775 DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 1834

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1835 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1894

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY
Sbjct: 1895 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 1954

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1955 LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2014

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNI 
Sbjct: 2015 PERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNID 2074

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQLT
Sbjct: 2075 IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQLT 2134

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD D
Sbjct: 2135 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDSD 2194

Query: 508  GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 567
            GNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKKEL
Sbjct: 2195 GNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKKEL 2254

Query: 568  ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 627
            ERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIERGEWIILKNANSCNPTVLDRINSLV
Sbjct: 2255 ERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIERGEWIILKNANSCNPTVLDRINSLV 2314

Query: 628  ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 687
            ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ
Sbjct: 2315 ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 2374

Query: 688  DGALCGKKDIELNDTRRFLALSGIPGAKL 716
            DGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 2375 DGAQCGKKDIELNDTQKFLALSGIPGAKL 2403

BLAST of CSPI05G06970 vs. ExPASy TrEMBL
Match: A0A5D3BVY6 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001100 PE=3 SV=1)

HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 671/691 (97.11%), Postives = 679/691 (98.26%), Query Frame = 0

Query: 25   LKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQ 84
            LK+L+ Q LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQD+NLFGIDPFFIEKGCQ
Sbjct: 1315 LKNLVLQ-LDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDNNLFGIDPFFIEKGCQ 1374

Query: 85   LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN 144
            LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN
Sbjct: 1375 LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN 1434

Query: 145  LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNA 204
            LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNA
Sbjct: 1435 LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNA 1494

Query: 205  ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED 264
            ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED
Sbjct: 1495 ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED 1554

Query: 265  DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE 324
            DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE
Sbjct: 1555 DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE 1614

Query: 325  GAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN 384
            GAPERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN
Sbjct: 1615 GAPERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN 1674

Query: 385  IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQ 444
            IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQ
Sbjct: 1675 IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQ 1734

Query: 445  LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD 504
            LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD
Sbjct: 1735 LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD 1794

Query: 505  RDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKK 564
             DGNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKK
Sbjct: 1795 SDGNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKK 1854

Query: 565  ELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINS 624
            ELERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIE GEWIILKNANSCNPTVLDRINS
Sbjct: 1855 ELERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIEHGEWIILKNANSCNPTVLDRINS 1914

Query: 625  LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW 684
            LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW
Sbjct: 1915 LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW 1974

Query: 685  LQDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            LQDGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 1975 LQDGAQCGKKDIELNDTQKFLALSGIPGAKL 2004

BLAST of CSPI05G06970 vs. ExPASy TrEMBL
Match: A0A5A7UWF5 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001400 PE=3 SV=1)

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 670/689 (97.24%), Postives = 678/689 (98.40%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQD+NLFGIDPFFIEKGCQLG
Sbjct: 1715 LLEKLKLDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDNNLFGIDPFFIEKGCQLG 1774

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS
Sbjct: 1775 DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 1834

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1835 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1894

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY
Sbjct: 1895 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 1954

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1955 LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2014

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA
Sbjct: 2015 PERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 2074

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQLT
Sbjct: 2075 IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQLT 2134

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD D
Sbjct: 2135 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDSD 2194

Query: 508  GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 567
            GNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKKEL
Sbjct: 2195 GNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKKEL 2254

Query: 568  ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 627
            ERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIE GEWIILKNANSCNPTVLDRINSLV
Sbjct: 2255 ERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIEHGEWIILKNANSCNPTVLDRINSLV 2314

Query: 628  ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 687
            ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ
Sbjct: 2315 ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 2374

Query: 688  DGALCGKKDIELNDTRRFLALSGIPGAKL 716
            DGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 2375 DGAQCGKKDIELNDTQKFLALSGIPGAKL 2403

BLAST of CSPI05G06970 vs. ExPASy TrEMBL
Match: A0A6J1JXY2 (Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1)

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 619/690 (89.71%), Postives = 651/690 (94.35%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +L +   ECS LV+LQSYGWGEL TAS+ SNTD MQDSN FGIDPF+IEKGC   
Sbjct: 1717 LLEKLKLGNAKLECSLLVKLQSYGWGELSTASDLSNTDYMQDSNHFGIDPFYIEKGCLPC 1776

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
             TGKYEF+APTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIV+LGQ SGH+VVRINLS
Sbjct: 1777 QTGKYEFLAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVSLGQISGHRVVRINLS 1836

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1837 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1896

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDEL+EDDY
Sbjct: 1897 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELVEDDY 1956

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNK+LHEEVML+KKFAQDG+PWEFNLRDVLRSCQIIEGA
Sbjct: 1957 LFICSSLYESIPKPLLSKLILFNKKLHEEVMLYKKFAQDGTPWEFNLRDVLRSCQIIEGA 2016

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVY+QRMRTA DRREVLRLYE+VFG+KY INPYPRVQLNS+FLIVGNIA
Sbjct: 2017 PERLRNYCFLNIVYIQRMRTACDRREVLRLYEQVFGSKYFINPYPRVQLNSQFLIVGNIA 2076

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            I RNSIQAC+VASSQLKILPGIRQSLEAVAHCI++QWMCILVG SSSGKTSLVRLLAQLT
Sbjct: 2077 IERNSIQACSVASSQLKILPGIRQSLEAVAHCIKHQWMCILVGSSSSGKTSLVRLLAQLT 2136

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRN  HVIDQVGFHVNKYCSVQIR SK+EFD  
Sbjct: 2137 GNVLNELNLSSTTDISELLGCFEQYDAIRNLRHVIDQVGFHVNKYCSVQIRSSKEEFDWV 2196

Query: 508  GNCIMTKWLSFSSKISFQLPS-SACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKE 567
            GN IMTKWLSFSSKI+FQL + SAC YAKNW RIV SLGLLVDIIKQLMS++ + PA K 
Sbjct: 2197 GNGIMTKWLSFSSKINFQLATGSACGYAKNWNRIVNSLGLLVDIIKQLMSYLNDAPANKV 2256

Query: 568  LERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSL 627
            LERC+KTVLKLEES QK PFSAKFEWV+GILVKAIE GEWIILKNANSCNPTVLDRINSL
Sbjct: 2257 LERCIKTVLKLEESEQKQPFSAKFEWVLGILVKAIEHGEWIILKNANSCNPTVLDRINSL 2316

Query: 628  VESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL 687
            VESCGSITINECGT+DGEPVVL+PHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL
Sbjct: 2317 VESCGSITINECGTVDGEPVVLYPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL 2376

Query: 688  QDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            QD A CGKKDIELNDTRRFLALSGIPGAKL
Sbjct: 2377 QDRAQCGKKDIELNDTRRFLALSGIPGAKL 2406

BLAST of CSPI05G06970 vs. NCBI nr
Match: XP_011659635.1 (midasin [Cucumis sativus] >KAE8646507.1 hypothetical protein Csa_015952 [Cucumis sativus])

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 686/689 (99.56%), Postives = 688/689 (99.85%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG
Sbjct: 1715 LLEKLKLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 1774

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS
Sbjct: 1775 DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 1834

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1835 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 1894

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY
Sbjct: 1895 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 1954

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1955 LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2014

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA
Sbjct: 2015 PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 2074

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT
Sbjct: 2075 IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 2134

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD
Sbjct: 2135 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 2194

Query: 508  GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 567
            GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL
Sbjct: 2195 GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 2254

Query: 568  ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 627
            ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV
Sbjct: 2255 ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 2314

Query: 628  ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 687
            ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ
Sbjct: 2315 ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 2374

Query: 688  DGALCGKKDIELNDTRRFLALSGIPGAKL 716
            DGALCGKKDIELNDTRRFLALSGIPGAKL
Sbjct: 2375 DGALCGKKDIELNDTRRFLALSGIPGAKL 2403

BLAST of CSPI05G06970 vs. NCBI nr
Match: XP_008462334.2 (PREDICTED: LOW QUALITY PROTEIN: midasin [Cucumis melo])

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 671/689 (97.39%), Postives = 678/689 (98.40%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQDSNLFGIDPFFIEKGCQLG
Sbjct: 1715 LLEKLKLDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDSNLFGIDPFFIEKGCQLG 1774

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS
Sbjct: 1775 DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 1834

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1835 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1894

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY
Sbjct: 1895 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 1954

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1955 LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2014

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNI 
Sbjct: 2015 PERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNID 2074

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQLT
Sbjct: 2075 IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQLT 2134

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD D
Sbjct: 2135 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDSD 2194

Query: 508  GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 567
            GNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKKEL
Sbjct: 2195 GNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKKEL 2254

Query: 568  ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 627
            ERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIERGEWIILKNANSCNPTVLDRINSLV
Sbjct: 2255 ERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIERGEWIILKNANSCNPTVLDRINSLV 2314

Query: 628  ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 687
            ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ
Sbjct: 2315 ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 2374

Query: 688  DGALCGKKDIELNDTRRFLALSGIPGAKL 716
            DGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 2375 DGAQCGKKDIELNDTQKFLALSGIPGAKL 2403

BLAST of CSPI05G06970 vs. NCBI nr
Match: TYK03913.1 (midasin [Cucumis melo var. makuwa])

HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 671/691 (97.11%), Postives = 679/691 (98.26%), Query Frame = 0

Query: 25   LKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQ 84
            LK+L+ Q LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQD+NLFGIDPFFIEKGCQ
Sbjct: 1315 LKNLVLQ-LDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDNNLFGIDPFFIEKGCQ 1374

Query: 85   LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN 144
            LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN
Sbjct: 1375 LGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRIN 1434

Query: 145  LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNA 204
            LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNA
Sbjct: 1435 LSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNA 1494

Query: 205  ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED 264
            ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED
Sbjct: 1495 ILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIED 1554

Query: 265  DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE 324
            DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE
Sbjct: 1555 DYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIE 1614

Query: 325  GAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN 384
            GAPERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN
Sbjct: 1615 GAPERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGN 1674

Query: 385  IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQ 444
            IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQ
Sbjct: 1675 IAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQ 1734

Query: 445  LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD 504
            LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD
Sbjct: 1735 LTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD 1794

Query: 505  RDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKK 564
             DGNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKK
Sbjct: 1795 SDGNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKK 1854

Query: 565  ELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINS 624
            ELERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIE GEWIILKNANSCNPTVLDRINS
Sbjct: 1855 ELERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIEHGEWIILKNANSCNPTVLDRINS 1914

Query: 625  LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW 684
            LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW
Sbjct: 1915 LVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHW 1974

Query: 685  LQDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            LQDGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 1975 LQDGAQCGKKDIELNDTQKFLALSGIPGAKL 2004

BLAST of CSPI05G06970 vs. NCBI nr
Match: KAA0059414.1 (midasin [Cucumis melo var. makuwa])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 670/689 (97.24%), Postives = 678/689 (98.40%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LDDTL ECSKLVRLQSYGWGELRT SNFSNTD MQD+NLFGIDPFFIEKGCQLG
Sbjct: 1715 LLEKLKLDDTLLECSKLVRLQSYGWGELRTVSNFSNTDYMQDNNLFGIDPFFIEKGCQLG 1774

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS
Sbjct: 1775 DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 1834

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1835 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1894

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY
Sbjct: 1895 DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 1954

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1955 LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2014

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVYVQRMRTA DRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA
Sbjct: 2015 PERLRNYCFLNIVYVQRMRTAADRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 2074

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSLVRLLAQLT
Sbjct: 2075 IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLVRLLAQLT 2134

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFD D
Sbjct: 2135 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDSD 2194

Query: 508  GNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKEL 567
            GNCIMTKWLSFSSKISFQLPSSACVYAKNW RIVCSLGLLVDIIKQLM FVQEVPAKKEL
Sbjct: 2195 GNCIMTKWLSFSSKISFQLPSSACVYAKNWNRIVCSLGLLVDIIKQLMLFVQEVPAKKEL 2254

Query: 568  ERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSLV 627
            ERCLKTVLKLEESNQKHPFSAKFEWV GILVKAIE GEWIILKNANSCNPTVLDRINSLV
Sbjct: 2255 ERCLKTVLKLEESNQKHPFSAKFEWVTGILVKAIEHGEWIILKNANSCNPTVLDRINSLV 2314

Query: 628  ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 687
            ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ
Sbjct: 2315 ESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWLQ 2374

Query: 688  DGALCGKKDIELNDTRRFLALSGIPGAKL 716
            DGA CGKKDIELNDT++FLALSGIPGAKL
Sbjct: 2375 DGAQCGKKDIELNDTQKFLALSGIPGAKL 2403

BLAST of CSPI05G06970 vs. NCBI nr
Match: XP_038899709.1 (midasin isoform X2 [Benincasa hispida])

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 640/690 (92.75%), Postives = 663/690 (96.09%), Query Frame = 0

Query: 28   LLEQ-RLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGCQLG 87
            LLE+ +LD+T+ ECSK  RLQSYGWGELRTA++ SNTD MQDSNLFGIDPFFIEKGC   
Sbjct: 1717 LLEKLKLDNTMLECSKFARLQSYGWGELRTATDLSNTDYMQDSNLFGIDPFFIEKGCLPC 1776

Query: 88   DTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRINLS 147
            DTGKYEF+APTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGH+VVRINLS
Sbjct: 1777 DTGKYEFLAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHRVVRINLS 1836

Query: 148  EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLNAIL 207
            EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQAL+EGCWVLLDELNLAPQSVLEGLNAIL
Sbjct: 1837 EQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALKEGCWVLLDELNLAPQSVLEGLNAIL 1896

Query: 208  DHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIEDDY 267
            DHRAEVFIPELALTFKCPPSFRVFACQNP+YQGGGRKGLPKSFLNRFTKVYLDEL+EDDY
Sbjct: 1897 DHRAEVFIPELALTFKCPPSFRVFACQNPTYQGGGRKGLPKSFLNRFTKVYLDELVEDDY 1956

Query: 268  LFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 327
            LFICSSLYESIPKPLLSKLILFNK+LHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA
Sbjct: 1957 LFICSSLYESIPKPLLSKLILFNKKLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQIIEGA 2016

Query: 328  PERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVGNIA 387
            PERLR+YCFLNIVYVQRMRTAGDRR VL+LYE+VFGAKY INPYPRVQLNSRFLIVGNIA
Sbjct: 2017 PERLRNYCFLNIVYVQRMRTAGDRRAVLQLYEQVFGAKYFINPYPRVQLNSRFLIVGNIA 2076

Query: 388  IGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLAQLT 447
            I RNSIQACNVASSQLKILPGIRQSLEAVAHCIQ+QWMCILVGPSSSGKTSL+RLLAQLT
Sbjct: 2077 IERNSIQACNVASSQLKILPGIRQSLEAVAHCIQHQWMCILVGPSSSGKTSLIRLLAQLT 2136

Query: 448  GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEFDRD 507
            GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVG++VNKYCSVQIR S K+FDRD
Sbjct: 2137 GNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGYYVNKYCSVQIRHSMKQFDRD 2196

Query: 508  GNCIMTKWLSFSSKISFQLP-SSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQEVPAKKE 567
            GN IMTKWLSFSSKISFQLP SS CVYAKNW RIV SLGLLVDIIKQLMS++QEVPA KE
Sbjct: 2197 GNSIMTKWLSFSSKISFQLPTSSVCVYAKNWNRIVNSLGLLVDIIKQLMSYLQEVPANKE 2256

Query: 568  LERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTVLDRINSL 627
            LE CLKTVLKL ESNQ+ PFSAKFEWV GILVKAIERGEWIILKNANSCNPTVLDRINSL
Sbjct: 2257 LESCLKTVLKLVESNQRQPFSAKFEWVTGILVKAIERGEWIILKNANSCNPTVLDRINSL 2316

Query: 628  VESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL 687
            VESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL
Sbjct: 2317 VESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIFMLQPHWL 2376

Query: 688  QDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            QD A CGKKDI LNDTRRFLALSGIPGAKL
Sbjct: 2377 QDEAQCGKKDI-LNDTRRFLALSGIPGAKL 2405

BLAST of CSPI05G06970 vs. TAIR 10
Match: AT1G67120.1 (ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription factor binding )

HSP 1 Score: 869.0 bits (2244), Expect = 2.6e-252
Identity = 438/699 (62.66%), Postives = 546/699 (78.11%), Query Frame = 0

Query: 24   YLKHLLEQRLDDTLHECSKLVRLQSYGWGELRTASNFSNTDSMQDSNLFGIDPFFIEKGC 83
            +L   LE    DTL    +L R++ YGWG+ +     S   S++   +FGIDPFFI KG 
Sbjct: 1695 FLLQQLELFASDTLP--LELSRMELYGWGDSKAICEKSK--SVRHEGMFGIDPFFISKGD 1754

Query: 84   QLGDTGKYEFMAPTTYKNALRVLRAMQLSKPVLLEGSPGVGKTSLIVALGQFSGHKVVRI 143
            +  + G +EF+APTT++N LRVLRAMQLSKP+LLEGSPGVGKTSLI+ALG++SGHKVVRI
Sbjct: 1755 ENPEIGGFEFLAPTTHRNVLRVLRAMQLSKPILLEGSPGVGKTSLILALGKYSGHKVVRI 1814

Query: 144  NLSEQTDMMDLLGSDLPVESDEGIKFAWSDGILLQALREGCWVLLDELNLAPQSVLEGLN 203
            NLSEQTDMMDLLGSDLPVESDE +KFAWSDGILLQAL+EG WVLLDELNLAPQSVLEGLN
Sbjct: 1815 NLSEQTDMMDLLGSDLPVESDEDMKFAWSDGILLQALKEGSWVLLDELNLAPQSVLEGLN 1874

Query: 204  AILDHRAEVFIPELALTFKCPPSFRVFACQNPSYQGGGRKGLPKSFLNRFTKVYLDELIE 263
            AILDHRA+VFIPEL  TF+CPP+FRVFACQNPS QGGGRKGLPKSFLNRFTKVY+DEL+E
Sbjct: 1875 AILDHRAQVFIPELGCTFECPPTFRVFACQNPSTQGGGRKGLPKSFLNRFTKVYVDELVE 1934

Query: 264  DDYLFICSSLYESIPKPLLSKLILFNKRLHEEVMLHKKFAQDGSPWEFNLRDVLRSCQII 323
            DDYLFIC SLY S+P PLLSKLI  N++LH+  ML++KF  DGSPWEFNLRDV+RSCQ +
Sbjct: 1935 DDYLFICRSLYPSVPSPLLSKLIALNRQLHDGTMLYRKFGHDGSPWEFNLRDVIRSCQFM 1994

Query: 324  EGAPERLRSYCFLNIVYVQRMRTAGDRREVLRLYEEVFGAKYLINPYPRVQLNSRFLIVG 383
            + A   L    FLN++Y+QRMRTA DR+EVLR+Y+ +F     INPYPRVQLN  +L+VG
Sbjct: 1995 QEAIHDLEVESFLNVLYIQRMRTATDRKEVLRIYKAIFDKTPSINPYPRVQLNPAYLVVG 2054

Query: 384  NIAIGRNSIQACNVASSQLKILPGIRQSLEAVAHCIQYQWMCILVGPSSSGKTSLVRLLA 443
              AI RN  Q+ N+AS QLK+LP IRQ+LEAVAHC+Q +W+CILVGPSSSGKTS++R+LA
Sbjct: 2055 TAAIKRNLNQS-NIASEQLKLLPEIRQNLEAVAHCVQNKWLCILVGPSSSGKTSVIRILA 2114

Query: 444  QLTGNVLNELNLSSTTDISELLGCFEQYDAIRNFHHVIDQVGFHVNKYCSVQIRCSKKEF 503
            QLTG  LNELNLSS TD S+LLGCFEQY+A RNF  V+ +V   V++Y S+ ++ S++  
Sbjct: 2115 QLTGYPLNELNLSSATDSSDLLGCFEQYNAFRNFRLVMTRVEHLVDEYNSLLLQSSQEAL 2174

Query: 504  DRDGNCIMTKWLSFSSKISFQLPSSACVYAKNWKRIVCSLGLLVDIIKQLMSFVQE--VP 563
              + + ++++WLS+ +KI   L  +   +  + +    +L  L ++++ L   ++E  +P
Sbjct: 2175 FSNRSGLVSRWLSYLNKIDSSLVENPLFFLNDSE----TLSTLEEVVEDLEQVLKEGVLP 2234

Query: 564  ---AKKELERCLKTVLKLEESNQKHPFSAKFEWVIGILVKAIERGEWIILKNANSCNPTV 623
               +KK LE+  KT+L+L+   +K   S KFEWV G+L+KAIE+GEW++LKNAN CNPTV
Sbjct: 2235 VSWSKKYLEQISKTILQLQTHEKKQ--STKFEWVTGMLIKAIEKGEWVVLKNANLCNPTV 2294

Query: 624  LDRINSLVESCGSITINECGTIDGEPVVLHPHANFRIFLTVNPIHGEVSRAMRNRGVEIF 683
            LDRINSLVE CGSITINECG ++GEPV + PH NFR+FL+VNP  GEVSRAMRNRGVE+F
Sbjct: 2295 LDRINSLVEPCGSITINECGIVNGEPVTVVPHPNFRLFLSVNPKFGEVSRAMRNRGVEVF 2354

Query: 684  MLQPHWL--QDGALCGKKDIELNDTRRFLALSGIPGAKL 716
            M+ PHW   +DG+ C  +++ L    RFLALSGIPG KL
Sbjct: 2355 MMGPHWQLNEDGSNC--EELVLRGVERFLALSGIPGYKL 2380