CSPI05G06960 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G06960
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMidasin
LocationChr5: 6175614 .. 6178704 (+)
RNA-Seq ExpressionCSPI05G06960
SyntenyCSPI05G06960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCGGATCATAAAAATTCTCAGAGAAGGCAGGGGTAATTGCCATAGTCTATGCGTAAAGGAGATTGAGCATATTAAAACAAAGCTTACAGAGTTGCATAAACAGTGGCAGACAATTTTCACCTGGCAGGATGGGCCTCTGGTGCAAGCAATGAGAGATGGTGATATCTTTCTTATAGATGAAATTTCTCTTGCTGATGATAGTGTGCTTGAAAGAATTAACAGCGTGTTGGAACCGGAAAGAAAGCTGGTAATATTACTAACCTATTCTATTTTTTTGAAAAGGAGATAACCTCTTTGCTAATATTAATATAATATTAATGAGACAAAAACTCATACTACAAGAGAATTATACAATGAACATGTAACCATGAACCAGGATACTAACCTTCTACGATGACCTGTTTTGCCATTTTGAGCTTAATCTTTTTTACATGCTCATATTTTTGTCTCATATAAGAAGTTGCGAGATTGTAATAGTTTTGCCGTTCTTCTAGCTTGTATTGTGAAACAGGTTTTTATTCCAGTAATAAGAAAAATATCCTTCTTGGGCTCTTTTCTGGAATTGGCATGCAGATATCATGCAAGTATATTTACTTGTGCCTGTAATTCTCTTTTCTTTTTTAAAAAGCTTGCTTTCAAATATAATTCAGCTGCTTGATAGAATAGCAAGATTTTTTCTTCAAAACTATTGATGGGCTGTCGTTTTTATAGGAAAGGCTAGTGAGTTGTGGATTTGCTACTGTAGTTTTTATCTTAGATATTCCAAGAAACTATTACACTTTAAACATTGGTGGGGAAACTTAGGGAAGAAGTTGTAGGACTACTGATGCTTTTGGCCTTGAAAGGTCTTATGACTTGTTTGGAAAATGTTTAAAGAGTTTCTCTTCATTTAAAAAAATTCTCGATATCATTTATGTTTTGTTTTCATTATTGGTTTATTTTCTGACCCATTAATTTGTTATTTTCTCTGTATTGGTTTTGTTTTTATTTTATCTTATATCTTTGTTCACAGTATAGATGTTATTTCCCCCTCTTTGCATCCTCTCTGCTTCCTCACGTGGGATAAAAGCAAACACAGTTGAATGCTATTTCATAGTTTTAACTTACATTAGGACAGTTAGCTTTACTGTTTTACATAGTTGACAATGTGATCGGCAGATGATGTGAAATTCAATTCTGGTTGGAATATTTCTGCAATTAGCAATCACTTCCCTCGATTAATCCTTTTTGCTCTATATACAAGTTTAAGATTCATGCTACTTTACAATTGCTGTACCTATTATGATACCATGTTGATTTGAATATTGCACTATTGTTTACTTTGTGTTTTATTTTGTAGGCTTTGGCTGAGAAAGGAGGAGAGTTTTTGGAAACAGTGACTGCTCATCCTGAATTCTCTCTCTTTGCAACAATGAATCCTGGTGGTGATTATGGCAAGAAGGAGTTATCTCCTGCACTTCGTAATCGGTTTACGGAGATATGGGTTCCTCCTGTTGGGGAGCTGGATGAGCTCAGGAGTATTGCGCTGACAAGGTATGGTTTCAACATGTTTTACCATTGCCCCAAATTTTATTGTAATCTTCCATGGATTTTACAAGCACATTTTCTTACAGGATTTCCAACCCTGGAGATACACATCTGGTGGATCTAATGCTAAATTTTTGGGAGGTATGTTCCCTTGCTTTATGAACAAAATTTGTTCTTTTAGGGATGTATTGCTTTTGCTTAGTGTATTTATAGTTTATCATTAATATTAAACATTACTCACCTTCGGTCTGGGTGAAACATTTCATTGATGAATGAAATAGATGGTTTCTGATACTCCAGCTAGTTAAGAAAGCACCTCTTTATTATGTCAATTCTGCAGCTTGGGTTATTTATTTCGATTTAATAGTTTGTCTTTCCTACTACAGTGGTTCAATCACCTTCAGTCTGGGAGAATGCTCACAGTGAGAGATCTACTTTCTTGGGTTTCCTTTATTGACTCAACTGAGATGAATCTAGGGCCGGAATATGCATTCCTTCATGGAGCATTCCTTATTCTTCTAGATGGTTTAAGTTTAGGTAAATCCTGCAGTGTTACTTCTTTATCTGCTTGTTAAGTTCCTTGCATTTCTTTAATGGTATTCTGTAGAATATTTATGCTTATGTCTAGGATCCCCAAATATGACAAATTATTTTATTGATGAAGAAACACAGTCAAAATCCTTAATAAGGGACTAAACTTGATAGAATAATAGGAATTGGATGGAAAAAGTGGAAGCAAGTAGAGTAAAAATAGGGTAGTAAGCAGCATGAAGCCTAGGGAACACATACTTTATTAAGCTGATGGCAAACAAGTGAATGCTATTATAATGTTCTTTAGAACAGAGAGGTTGAGGCTGGTCTGCTTCAAGTTTTAGTGTATCATTTCTTGATTCTCGCCTGTGTTTTATCTCTTGTAATTACTGTGAAGTCAAGGAAAAGTTACGTTCTAATGTTAAAAATTGTTGGAAGAGACTTCAGGTGTATAATTGATAAGAGCCTTCTTCATTAATGGGTGTATTGGTTGCTTGGTTATCAAATGTGTTAGGATGGGAAACGATCTTACTTGAATAGGATCAGTTCTATAGGTTGTACATTTGTTTCCTTAAGTTCTAATTAGTCAAGAACCAACAGGCCATGGTCCGGCGGCTATATTAAAAATATGTGTTGGATGGAAAACCTTCCCCCTCCCAAGTTGTGGGAACTAAAAGAAACTGGAGCCGCATGCTTATTTTTGACCTGTGAATTGTGTTATTGTCATGTCCTTTCCTTATGTTGGTTGTGTATGATACTTGCTTGTATGTTATTAGCCATTTGTTTACAGATGTGTGGCTTTTTTTCTTCTTTTATAATGAAATTCATTTTCTTATATTGATTCTCAGGGACTGGTATGTCAAAAAGAGATGCAGAAGAATTGCGCAAAAGATGCTTTTCATTTCTTTTAGAGAAACTAAAGGTTGTCAATCTTCTGTCAACTTCATGTTATATGACTTCGGTATAATTGCTCTGGAGTTGTTCTCTGTGTTCTTTTCTCTTGATAGTTAATTGCTATAAAGCAATAA

mRNA sequence

ATGGACCGGATCATAAAAATTCTCAGAGAAGGCAGGGGTAATTGCCATAGTCTATGCGTAAAGGAGATTGAGCATATTAAAACAAAGCTTACAGAGTTGCATAAACAGTGGCAGACAATTTTCACCTGGCAGGATGGGCCTCTGGTGCAAGCAATGAGAGATGGTGATATCTTTCTTATAGATGAAATTTCTCTTGCTGATGATAGTGTGCTTGAAAGAATTAACAGCGTGTTGGAACCGGAAAGAAAGCTGGCTTTGGCTGAGAAAGGAGGAGAGTTTTTGGAAACAGTGACTGCTCATCCTGAATTCTCTCTCTTTGCAACAATGAATCCTGGTGGTGATTATGGCAAGAAGGAGTTATCTCCTGCACTTCGTAATCGGTTTACGGAGATATGGGTTCCTCCTGTTGGGGAGCTGGATGAGCTCAGGAGTATTGCGCTGACAAGGATTTCCAACCCTGGAGATACACATCTGGTGGATCTAATGCTAAATTTTTGGGAGTGGTTCAATCACCTTCAGTCTGGGAGAATGCTCACAGTGAGAGATCTACTTTCTTGGGTTTCCTTTATTGACTCAACTGAGATGAATCTAGGGCCGGAATATGCATTCCTTCATGGAGCATTCCTTATTCTTCTAGATGGTTTAAGTTTAGGGACTGGTATGTCAAAAAGAGATGCAGAAGAATTGCGCAAAAGATGCTTTTCATTTCTTTTAGAGAAACTAAAGTTAATTGCTATAAAGCAATAA

Coding sequence (CDS)

ATGGACCGGATCATAAAAATTCTCAGAGAAGGCAGGGGTAATTGCCATAGTCTATGCGTAAAGGAGATTGAGCATATTAAAACAAAGCTTACAGAGTTGCATAAACAGTGGCAGACAATTTTCACCTGGCAGGATGGGCCTCTGGTGCAAGCAATGAGAGATGGTGATATCTTTCTTATAGATGAAATTTCTCTTGCTGATGATAGTGTGCTTGAAAGAATTAACAGCGTGTTGGAACCGGAAAGAAAGCTGGCTTTGGCTGAGAAAGGAGGAGAGTTTTTGGAAACAGTGACTGCTCATCCTGAATTCTCTCTCTTTGCAACAATGAATCCTGGTGGTGATTATGGCAAGAAGGAGTTATCTCCTGCACTTCGTAATCGGTTTACGGAGATATGGGTTCCTCCTGTTGGGGAGCTGGATGAGCTCAGGAGTATTGCGCTGACAAGGATTTCCAACCCTGGAGATACACATCTGGTGGATCTAATGCTAAATTTTTGGGAGTGGTTCAATCACCTTCAGTCTGGGAGAATGCTCACAGTGAGAGATCTACTTTCTTGGGTTTCCTTTATTGACTCAACTGAGATGAATCTAGGGCCGGAATATGCATTCCTTCATGGAGCATTCCTTATTCTTCTAGATGGTTTAAGTTTAGGGACTGGTATGTCAAAAAGAGATGCAGAAGAATTGCGCAAAAGATGCTTTTCATTTCTTTTAGAGAAACTAAAGTTAATTGCTATAAAGCAATAA

Protein sequence

MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTVRDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEKLKLIAIKQ*
Homology
BLAST of CSPI05G06960 vs. ExPASy Swiss-Prot
Match: A0A1P8AUY4 (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.2e-85
Identity = 145/223 (65.02%), Postives = 186/223 (83.41%), Query Frame = 0

Query: 23   IEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPER 82
            +E I+  +  L+++W+ IF WQDGPLV+AMR G+I L+DEISLADDSVLER+NSVLE +R
Sbjct: 1482 LEKIRNNMVMLYQKWRAIFVWQDGPLVEAMRAGNIVLVDEISLADDSVLERMNSVLETDR 1541

Query: 83   KLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDEL 142
            KL+LAEKGG  LE V AH +F + ATMNPGGDYGKKELSPALRNRFTEIWVPP+ + +EL
Sbjct: 1542 KLSLAEKGGPVLEEVVAHEDFFVLATMNPGGDYGKKELSPALRNRFTEIWVPPITDTEEL 1601

Query: 143  RSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTVRDLLSWVSFIDSTEMNLGPEYA 202
            RSIA + +S+  ++++VD ++NFWEWFN L +GR LTVRDLLSWV+F++    +LGP YA
Sbjct: 1602 RSIAFSGLSSLKESNVVDPIINFWEWFNRLHTGRTLTVRDLLSWVAFVNMATESLGPAYA 1661

Query: 203  FLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEKLKLIA 246
             LHGAFL+LLDGLSLGTG S RD ++LR++CF+FLL++L+L A
Sbjct: 1662 ILHGAFLVLLDGLSLGTGFSGRDGQDLREKCFAFLLQQLELFA 1704

BLAST of CSPI05G06960 vs. ExPASy Swiss-Prot
Match: Q869L3 (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2)

HSP 1 Score: 216.5 bits (550), Expect = 3.5e-55
Identity = 106/230 (46.09%), Postives = 158/230 (68.70%), Query Frame = 0

Query: 17   SLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINS 76
            SL  ++++ +  ++ + +  + ++F W DGPLV+AM+ GD FLIDEISLA+D+VLER+NS
Sbjct: 1540 SLLTEQLKQLAIEIDKTYSSYCSLFNWVDGPLVEAMKQGDYFLIDEISLAEDAVLERLNS 1599

Query: 77   VLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPV 136
            VLEP R L LAEKGG  +E +  H EF + ATMNPGGD+GKKELSPA+RNRFTEIWVP +
Sbjct: 1600 VLEPSRLLVLAEKGGVEIEELRGHKEFRILATMNPGGDFGKKELSPAMRNRFTEIWVPAI 1659

Query: 137  GELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSG-RMLTVRDLLSWVSFID--ST 196
                +L  I   R ++P       LMLNF E+   +Q   R++++RD+LSW+SF++    
Sbjct: 1660 SSHQDLLQIIEERFTSPQLKGKGSLMLNFIEYLLMIQKNKRVISLRDILSWISFMNLCMD 1719

Query: 197  EMNLGPEYAFLHGAFLILLDGLSLGT-GMSKRDAEELRKRCFSFLLEKLK 243
            +  L P  +++HGA L+LLDG  +G+   S+ +  +LR+ C   L+++++
Sbjct: 1720 KQLLSPNESYIHGACLVLLDGFGMGSNSSSESEGLKLREACLIRLIDQIE 1769

BLAST of CSPI05G06960 vs. ExPASy Swiss-Prot
Match: Q12019 (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.0e-46
Identity = 100/226 (44.25%), Postives = 137/226 (60.62%), Query Frame = 0

Query: 24   EHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPERK 83
            E ++ ++ +L      +F W DGPL+QAMR G+ FL+DEISLADDSVLER+NSVLEPER 
Sbjct: 1455 EDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERS 1514

Query: 84   LALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDELR 143
            L LAE+G      VTA   F  FATMNPGGDYGKKELSPALRNRFTEIWVP + + +++ 
Sbjct: 1515 LLLAEQGSSD-SLVTASENFQFFATMNPGGDYGKKELSPALRNRFTEIWVPSMEDFNDVN 1574

Query: 144  SIALTRISNPGDTHLVDLMLNFWEWFNHLQSG-----RMLTVRDLLSWVSFIDSTEMNL- 203
             I  +R+       L + ++ F EWF     G      ++++RD+L+WV FI+     + 
Sbjct: 1575 MIVSSRLLE-DLKDLANPIVKFSEWFGKKLGGGNATSGVISLRDILAWVEFINKVFPKIQ 1634

Query: 204  GPEYAFLHGAFLILLDGLSLGT----GMSKRDAEELRKRCFSFLLE 240
                A + GA ++ +D L          ++ D + LR  C   LL+
Sbjct: 1635 NKSTALIQGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLK 1678

BLAST of CSPI05G06960 vs. ExPASy Swiss-Prot
Match: Q9NU22 (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 3.3e-45
Identity = 105/230 (45.65%), Postives = 142/230 (61.74%), Query Frame = 0

Query: 40   IFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPERKLALAEKGG-----EFL 99
            +F W DGPLVQAM++   FL+DEISLADDSVLER+NSVLE E+ L LAEKG        +
Sbjct: 1447 LFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEI 1506

Query: 100  ETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDELRSI--------- 159
            E +TA  +F + ATMNPGGD+GKKELSPALRNRFTEIW P     ++L  I         
Sbjct: 1507 ELLTAGKKFRILATMNPGGDFGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGL 1566

Query: 160  ALTRISNPGDTHLVDLMLNFWEWFNHLQSGR--MLTVRDLLSWVSFIDSTEMNLG---PE 219
             L RI +P  + + ++ML+F +W  H + GR  ++++RD+LSWV+F++          PE
Sbjct: 1567 CLGRI-DPKGSDIPEVMLDFIDWLTHQEFGRKCVVSIRDILSWVNFMNKMGEEAALKRPE 1626

Query: 220  Y-----AFLHGAFLILLDGLSLG-TGMSKRDAEELRKRCFSFLLEKLKLI 245
                  +F+H A L+ +DG+  G T      A   RK C  FL+++L  I
Sbjct: 1627 IISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKRLAKI 1675

BLAST of CSPI05G06960 vs. ExPASy Swiss-Prot
Match: O94248 (Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 7.1e-40
Identity = 93/211 (44.08%), Postives = 132/211 (62.56%), Query Frame = 0

Query: 36   QWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPERKLALAEKGGEFLE 95
            +++++F W DG LV AM+ GD FL+DEISLADDSVLER+NSVLE  R L L E     + 
Sbjct: 1291 KYRSLFEWHDGALVTAMKQGDFFLLDEISLADDSVLERLNSVLELSRTLTLVEHSNAAV- 1350

Query: 96   TVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDELRSIALTRISNPGD 155
            ++TA   F+ FATMNPGGDYGKKELSPALRNRFTEIWVPP+ + +++  I   ++ N   
Sbjct: 1351 SLTAKDGFAFFATMNPGGDYGKKELSPALRNRFTEIWVPPMVDTEDILKIVEGKLHN-NK 1410

Query: 156  THLVDLMLNFWEWF-NHLQSGRMLTVRDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDG 215
              L   ++ + +W  N      ++++RD+LS V FI++ E+ L       +   ++ +D 
Sbjct: 1411 IELARPLVEYAKWHANEYLYTDVISIRDVLSAVEFINACEI-LDLNLVLFNAVSMVFIDA 1470

Query: 216  L------SLGTGMSKRDAEELRKRCFSFLLE 240
            L      SL   ++   AE  R+RCF+ L E
Sbjct: 1471 LGSFTTFSLSNNLASLHAE--RQRCFAKLNE 1496

BLAST of CSPI05G06960 vs. ExPASy TrEMBL
Match: A0A0A0KCN0 (Midasin OS=Cucumis sativus OX=3659 GN=Csa_7G450640 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.6e-138
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI
Sbjct: 1095 MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 1154

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1155 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1214

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV
Sbjct: 1215 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 1274

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1275 RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1334

Query: 241  LKL 244
            LKL
Sbjct: 1335 LKL 1337

BLAST of CSPI05G06960 vs. ExPASy TrEMBL
Match: A0A1S3CGN9 (Midasin OS=Cucumis melo OX=3656 GN=LOC103500710 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.0e-134
Identity = 236/243 (97.12%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRI+KILREGRGNCHSLC+KEIEHIKTKLTEL+KQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1479 MDRIMKILREGRGNCHSLCIKEIEHIKTKLTELYKQWQTIFTWQDGPLVQAMRDGDMFLI 1538

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1539 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1598

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG THLVDLML+FWEWFNHLQSGRMLTV
Sbjct: 1599 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGTHLVDLMLSFWEWFNHLQSGRMLTV 1658

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTE NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1659 RDLLSWVSFIDSTERNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1718

Query: 241  LKL 244
            LKL
Sbjct: 1719 LKL 1721

BLAST of CSPI05G06960 vs. ExPASy TrEMBL
Match: A0A5A7UWF5 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001400 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.3e-134
Identity = 235/243 (96.71%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRI+K+LREGRGNCHSLC+KEIEHIKTKLTEL+KQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1479 MDRIMKVLREGRGNCHSLCIKEIEHIKTKLTELYKQWQTIFTWQDGPLVQAMRDGDMFLI 1538

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1539 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1598

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG THLVDLML+FWEWFNHLQSGRMLTV
Sbjct: 1599 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGTHLVDLMLSFWEWFNHLQSGRMLTV 1658

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTE NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1659 RDLLSWVSFIDSTERNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1718

Query: 241  LKL 244
            LKL
Sbjct: 1719 LKL 1721

BLAST of CSPI05G06960 vs. ExPASy TrEMBL
Match: A0A6J1JXY2 (Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.3e-124
Identity = 221/243 (90.95%), Postives = 228/243 (93.83%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            +DRIIK LRE  G+C   C+KE+E IK KLTELHKQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1481 LDRIIKTLREVSGHCQRPCIKELELIKKKLTELHKQWQTIFTWQDGPLVQAMRDGDMFLI 1540

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1541 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1600

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELR+IALTRISNP  THLVDLML FWEWFN LQSGRMLTV
Sbjct: 1601 SPALRNRFTEIWVPPVGELDELRNIALTRISNPRVTHLVDLMLTFWEWFNQLQSGRMLTV 1660

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTE  LGPEYAFLHGAFL+LLDGLSLGTGM KRDAEELRKRCFSFLLEK
Sbjct: 1661 RDLLSWVSFIDSTERKLGPEYAFLHGAFLVLLDGLSLGTGMLKRDAEELRKRCFSFLLEK 1720

Query: 241  LKL 244
            LKL
Sbjct: 1721 LKL 1723

BLAST of CSPI05G06960 vs. ExPASy TrEMBL
Match: A0A6J1DE15 (Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.3e-124
Identity = 216/243 (88.89%), Postives = 234/243 (96.30%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            ++RI+++LRE RGNC+SL +KE+EHIK KLTELHKQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1480 LERIMEVLRESRGNCNSLSIKELEHIKMKLTELHKQWQTIFTWQDGPLVQAMRDGDMFLI 1539

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLER+NSVLEPER LALAEKGG+FLETVTAHPEF LFATMNPGGDYGKKEL
Sbjct: 1540 DEISLADDSVLERLNSVLEPERTLALAEKGGQFLETVTAHPEFFLFATMNPGGDYGKKEL 1599

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGEL+ELRSIALTRISNP  THLVDLML+FWEWFN L+SGR+LTV
Sbjct: 1600 SPALRNRFTEIWVPPVGELNELRSIALTRISNPRVTHLVDLMLSFWEWFNQLRSGRVLTV 1659

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWV+FIDSTE NLGPEYAFLHGAFL+LLDGLSLGTGMSKRDAEELR+RCFSFLLEK
Sbjct: 1660 RDLLSWVAFIDSTERNLGPEYAFLHGAFLVLLDGLSLGTGMSKRDAEELRERCFSFLLEK 1719

Query: 241  LKL 244
            LKL
Sbjct: 1720 LKL 1722

BLAST of CSPI05G06960 vs. NCBI nr
Match: XP_011659635.1 (midasin [Cucumis sativus] >KAE8646507.1 hypothetical protein Csa_015952 [Cucumis sativus])

HSP 1 Score: 501.1 bits (1289), Expect = 5.4e-138
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI
Sbjct: 1479 MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 1538

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1539 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1598

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV
Sbjct: 1599 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 1658

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1659 RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1718

Query: 241  LKL 244
            LKL
Sbjct: 1719 LKL 1721

BLAST of CSPI05G06960 vs. NCBI nr
Match: XP_008462334.2 (PREDICTED: LOW QUALITY PROTEIN: midasin [Cucumis melo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.1e-134
Identity = 236/243 (97.12%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRI+KILREGRGNCHSLC+KEIEHIKTKLTEL+KQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1479 MDRIMKILREGRGNCHSLCIKEIEHIKTKLTELYKQWQTIFTWQDGPLVQAMRDGDMFLI 1538

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1539 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1598

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG THLVDLML+FWEWFNHLQSGRMLTV
Sbjct: 1599 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGTHLVDLMLSFWEWFNHLQSGRMLTV 1658

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTE NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1659 RDLLSWVSFIDSTERNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1718

Query: 241  LKL 244
            LKL
Sbjct: 1719 LKL 1721

BLAST of CSPI05G06960 vs. NCBI nr
Match: KAA0059414.1 (midasin [Cucumis melo var. makuwa])

HSP 1 Score: 488.8 bits (1257), Expect = 2.8e-134
Identity = 235/243 (96.71%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            MDRI+K+LREGRGNCHSLC+KEIEHIKTKLTEL+KQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1479 MDRIMKVLREGRGNCHSLCIKEIEHIKTKLTELYKQWQTIFTWQDGPLVQAMRDGDMFLI 1538

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL
Sbjct: 1539 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 1598

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG THLVDLML+FWEWFNHLQSGRMLTV
Sbjct: 1599 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGTHLVDLMLSFWEWFNHLQSGRMLTV 1658

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDSTE NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK
Sbjct: 1659 RDLLSWVSFIDSTERNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 1718

Query: 241  LKL 244
            LKL
Sbjct: 1719 LKL 1721

BLAST of CSPI05G06960 vs. NCBI nr
Match: XP_038899709.1 (midasin isoform X2 [Benincasa hispida])

HSP 1 Score: 468.4 bits (1204), Expect = 3.9e-128
Identity = 225/243 (92.59%), Postives = 233/243 (95.88%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            +DRI+KILREGR NC  LCV+E+EHIK KL ELHKQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1481 LDRIMKILREGRENCQRLCVEELEHIKKKLAELHKQWQTIFTWQDGPLVQAMRDGDMFLI 1540

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEF LFATMNPGGDYGKKEL
Sbjct: 1541 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFCLFATMNPGGDYGKKEL 1600

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG  HLVDLMLNFWEWFNHL+SGRM+TV
Sbjct: 1601 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGKHLVDLMLNFWEWFNHLRSGRMITV 1660

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDST  NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCF+FLLEK
Sbjct: 1661 RDLLSWVSFIDSTSRNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFTFLLEK 1720

Query: 241  LKL 244
            LKL
Sbjct: 1721 LKL 1723

BLAST of CSPI05G06960 vs. NCBI nr
Match: XP_038899710.1 (midasin isoform X3 [Benincasa hispida])

HSP 1 Score: 468.4 bits (1204), Expect = 3.9e-128
Identity = 225/243 (92.59%), Postives = 233/243 (95.88%), Query Frame = 0

Query: 1    MDRIIKILREGRGNCHSLCVKEIEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLI 60
            +DRI+KILREGR NC  LCV+E+EHIK KL ELHKQWQTIFTWQDGPLVQAMRDGD+FLI
Sbjct: 1280 LDRIMKILREGRENCQRLCVEELEHIKKKLAELHKQWQTIFTWQDGPLVQAMRDGDMFLI 1339

Query: 61   DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKEL 120
            DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEF LFATMNPGGDYGKKEL
Sbjct: 1340 DEISLADDSVLERINSVLEPERKLALAEKGGEFLETVTAHPEFCLFATMNPGGDYGKKEL 1399

Query: 121  SPALRNRFTEIWVPPVGELDELRSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTV 180
            SPALRNRFTEIWVPPVGELDELRSIALTRISNPG  HLVDLMLNFWEWFNHL+SGRM+TV
Sbjct: 1400 SPALRNRFTEIWVPPVGELDELRSIALTRISNPGGKHLVDLMLNFWEWFNHLRSGRMITV 1459

Query: 181  RDLLSWVSFIDSTEMNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEK 240
            RDLLSWVSFIDST  NLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCF+FLLEK
Sbjct: 1460 RDLLSWVSFIDSTSRNLGPEYAFLHGAFLILLDGLSLGTGMSKRDAEELRKRCFTFLLEK 1519

Query: 241  LKL 244
            LKL
Sbjct: 1520 LKL 1522

BLAST of CSPI05G06960 vs. TAIR 10
Match: AT1G67120.1 (ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription factor binding )

HSP 1 Score: 316.2 bits (809), Expect = 2.3e-86
Identity = 145/223 (65.02%), Postives = 186/223 (83.41%), Query Frame = 0

Query: 23   IEHIKTKLTELHKQWQTIFTWQDGPLVQAMRDGDIFLIDEISLADDSVLERINSVLEPER 82
            +E I+  +  L+++W+ IF WQDGPLV+AMR G+I L+DEISLADDSVLER+NSVLE +R
Sbjct: 1482 LEKIRNNMVMLYQKWRAIFVWQDGPLVEAMRAGNIVLVDEISLADDSVLERMNSVLETDR 1541

Query: 83   KLALAEKGGEFLETVTAHPEFSLFATMNPGGDYGKKELSPALRNRFTEIWVPPVGELDEL 142
            KL+LAEKGG  LE V AH +F + ATMNPGGDYGKKELSPALRNRFTEIWVPP+ + +EL
Sbjct: 1542 KLSLAEKGGPVLEEVVAHEDFFVLATMNPGGDYGKKELSPALRNRFTEIWVPPITDTEEL 1601

Query: 143  RSIALTRISNPGDTHLVDLMLNFWEWFNHLQSGRMLTVRDLLSWVSFIDSTEMNLGPEYA 202
            RSIA + +S+  ++++VD ++NFWEWFN L +GR LTVRDLLSWV+F++    +LGP YA
Sbjct: 1602 RSIAFSGLSSLKESNVVDPIINFWEWFNRLHTGRTLTVRDLLSWVAFVNMATESLGPAYA 1661

Query: 203  FLHGAFLILLDGLSLGTGMSKRDAEELRKRCFSFLLEKLKLIA 246
             LHGAFL+LLDGLSLGTG S RD ++LR++CF+FLL++L+L A
Sbjct: 1662 ILHGAFLVLLDGLSLGTGFSGRDGQDLREKCFAFLLQQLELFA 1704

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8AUY43.2e-8565.02Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1[more]
Q869L33.5e-5546.09Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2[more]
Q120193.0e-4644.25Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MD... [more]
Q9NU223.3e-4545.65Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2[more]
O942487.1e-4044.08Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1... [more]
Match NameE-valueIdentityDescription
A0A0A0KCN02.6e-138100.00Midasin OS=Cucumis sativus OX=3659 GN=Csa_7G450640 PE=3 SV=1[more]
A0A1S3CGN91.0e-13497.12Midasin OS=Cucumis melo OX=3656 GN=LOC103500710 PE=3 SV=1[more]
A0A5A7UWF51.3e-13496.71Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001400 PE=3 ... [more]
A0A6J1JXY26.3e-12490.95Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1[more]
A0A6J1DE156.3e-12488.89Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011659635.15.4e-138100.00midasin [Cucumis sativus] >KAE8646507.1 hypothetical protein Csa_015952 [Cucumis... [more]
XP_008462334.22.1e-13497.12PREDICTED: LOW QUALITY PROTEIN: midasin [Cucumis melo][more]
KAA0059414.12.8e-13496.71midasin [Cucumis melo var. makuwa][more]
XP_038899709.13.9e-12892.59midasin isoform X2 [Benincasa hispida][more]
XP_038899710.13.9e-12892.59midasin isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G67120.12.3e-8665.02ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 15..132
e-value: 7.2E-27
score: 96.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 37..189
IPR011704ATPase, dynein-related, AAA domainPFAMPF07728AAA_5coord: 28..128
e-value: 2.2E-16
score: 60.1
NoneNo IPR availablePANTHERPTHR48103MIDASIN-RELATEDcoord: 41..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G06960.1CSPI05G06960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000027 ribosomal large subunit assembly
biological_process GO:0006364 rRNA processing
cellular_component GO:0005634 nucleus
cellular_component GO:0030687 preribosome, large subunit precursor
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity