CSPI05G06950 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G06950
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	3-isopropylmalate dehydratase small subunit 3-like
Location	Chr5: 6174156 .. 6174386 (+)
RNA-Seq Expression	CSPI05G06950
Synteny	CSPI05G06950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACCAAATATTCCATCATAATTGTCGGTGACAACTTCGGTTGTGGTTCTTCACGGGAGCACGTGCCGGTTGCATTGGGAGTGTCCGACGCCGTGGCGGTAGTGGCTGAATCTTATGCGCATATTTTCCTCACAGCAAACTCCATGGCAACCAGAGAGATTTATCCATTGGAATCAGAGACTAGGATTTCACGTATTTTAAAATTAAACAATAATAATAATAACTAG

mRNA sequence

ATGAAAACCAAATATTCCATCATAATTGTCGGTGACAACTTCGGTTGTGGTTCTTCACGGGAGCACGTGCCGGTTGCATTGGGAGTGTCCGACGCCGTGGCGGTAGTGGCTGAATCTTATGCGCATATTTTCCTCACAGCAAACTCCATGGCAACCAGAGAGATTTATCCATTGGAATCAGAGACTAGGATTTCACGTATTTTAAAATTAAACAATAATAATAATAACTAG

Coding sequence (CDS)

ATGAAAACCAAATATTCCATCATAATTGTCGGTGACAACTTCGGTTGTGGTTCTTCACGGGAGCACGTGCCGGTTGCATTGGGAGTGTCCGACGCCGTGGCGGTAGTGGCTGAATCTTATGCGCATATTTTCCTCACAGCAAACTCCATGGCAACCAGAGAGATTTATCCATTGGAATCAGAGACTAGGATTTCACGTATTTTAAAATTAAACAATAATAATAATAACTAG

Protein sequence

MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLESETRISRILKLNNNNNN*

Homology

[Close]

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot
Match: Q9ZW85 (3-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN=SSU1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.2e-17
Identity = 45/64 (70.31%), Postives = 52/64 (81.25%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII G+NFGCGSSREH PV LG + A AVVA+SYA IF   NS+AT E+YPL+S
Sbjct: 127 MKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFF-RNSVATGEVYPLDS 186

Query: 61  ETRI 65
           E R+
Sbjct: 187 EVRV 189

[Close]

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot
Match: Q9LYT7 (3-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN=SSU3 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.0e-17
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MK+KYS+II GDNFGCGSSREH PV LG + A AVVAESYA IF   N +AT EI+PLES
Sbjct: 130 MKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESYARIFF-RNCVATGEIFPLES 189

Query: 61  ETRI 65
           E RI
Sbjct: 190 EVRI 192

[Close]

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot
Match: Q9ZW84 (3-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN=SSU2 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.9e-16
Identity = 42/63 (66.67%), Postives = 51/63 (80.95%), Query Frame = 0


Query: 2   KTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLESE 61
           ++KYSIII G+NFGCGSSREH PV LG + A A+VAESYA IF   NS+AT E++PLESE
Sbjct: 134 RSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFF-RNSVATGEVFPLESE 193

Query: 62  TRI 65
            R+
Sbjct: 194 VRV 195

[Close]

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot
Match: Q7M887 (3-isopropylmalate dehydratase small subunit OS=Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) OX=273121 GN=leuD PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.2e-07
Identity = 25/42 (59.52%), Postives = 31/42 (73.81%), Query Frame = 0


Query: 3  TKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIF 45
          TK   I+ G+NFGCGSSREH PVAL  +   AV+A+S+A IF
Sbjct: 51 TKGDFIVAGENFGCGSSREHAPVALKTAGVSAVIAKSFARIF 92

[Close]

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot
Match: A8ETJ8 (3-isopropylmalate dehydratase small subunit OS=Arcobacter butzleri (strain RM4018) OX=367737 GN=leuD PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.2e-07
Identity = 25/41 (60.98%), Postives = 30/41 (73.17%), Query Frame = 0


Query: 4  KYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIF 45
          K  II+ G+NFGCGSSREH P+AL  +   AVVA S+A IF
Sbjct: 52 KGDIIVAGENFGCGSSREHAPIALKAAGIAAVVAPSFARIF 92

[Close]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL
Match: A0A0A0KPA8 (Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G162650 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 5.6e-31
Identity = 73/76 (96.05%), Postives = 74/76 (97.37%), Query Frame = 0


Query: 1  MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
          MKTKYSIIIVGDNFGCGSSREHVPV LGVS AVAVVAESYAHIFLTANS+ATREIYPLES
Sbjct: 1  MKTKYSIIIVGDNFGCGSSREHVPVVLGVSGAVAVVAESYAHIFLTANSVATREIYPLES 60

Query: 61 ETRISRILKLNNNNNN 77
          ETRISRILKLNNNNNN
Sbjct: 61 ETRISRILKLNNNNNN 76

[Close]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL
Match: A0A5A7T900 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00620 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.4e-19
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           ETRI
Sbjct: 194 ETRI 196

[Close]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL
Match: A0A0A0KPB2 (Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G165170 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.4e-19
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 129 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 188

Query: 61  ETRI 65
           ETRI
Sbjct: 189 ETRI 191

[Close]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL
Match: A0A1S3AT32 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo OX=3656 GN=LOC103482595 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.4e-19
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           ETRI
Sbjct: 194 ETRI 196

[Close]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL
Match: A0A6J1ID74 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucurbita maxima OX=3661 GN=LOC111471515 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.2e-18
Identity = 53/64 (82.81%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           E RI
Sbjct: 194 EIRI 196

[Close]

BLAST of CSPI05G06950 vs. NCBI nr
Match: XP_004152394.1 (3-isopropylmalate dehydratase small subunit 3 [Cucumis sativus] >KGN50272.1 hypothetical protein Csa_000662 [Cucumis sativus])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 129 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 188

Query: 61  ETRI 65
           ETRI
Sbjct: 189 ETRI 191

[Close]

BLAST of CSPI05G06950 vs. NCBI nr
Match: XP_038876127.1 (3-isopropylmalate dehydratase small subunit 1-like [Benincasa hispida])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 167 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 226

Query: 61  ETRI 65
           ETRI
Sbjct: 227 ETRI 229

[Close]

BLAST of CSPI05G06950 vs. NCBI nr
Match: XP_008437054.1 (PREDICTED: 3-isopropylmalate dehydratase small subunit 3-like [Cucumis melo] >KAA0039730.1 3-isopropylmalate dehydratase small subunit 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 54/64 (84.38%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           ETRI
Sbjct: 194 ETRI 196

[Close]

BLAST of CSPI05G06950 vs. NCBI nr
Match: XP_022922281.1 (3-isopropylmalate dehydratase small subunit 3-like [Cucurbita moschata])

HSP 1 Score: 100.5 bits (249), Expect = 6.6e-18
Identity = 53/64 (82.81%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           E RI
Sbjct: 194 EIRI 196

[Close]

BLAST of CSPI05G06950 vs. NCBI nr
Match: XP_022972989.1 (3-isopropylmalate dehydratase small subunit 3-like [Cucurbita maxima])

HSP 1 Score: 100.5 bits (249), Expect = 6.6e-18
Identity = 53/64 (82.81%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII GDNFGCGSSREH PVALG S AVAVVAESYA IF   NS+AT EIYPLES
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           E RI
Sbjct: 194 EIRI 196

[Close]

BLAST of CSPI05G06950 vs. TAIR 10
Match: AT2G43090.1 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 90.1 bits (222), Expect = 8.3e-19
Identity = 45/64 (70.31%), Postives = 52/64 (81.25%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MKTKYSIII G+NFGCGSSREH PV LG + A AVVA+SYA IF   NS+AT E+YPL+S
Sbjct: 127 MKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFF-RNSVATGEVYPLDS 186

Query: 61  ETRI 65
           E R+
Sbjct: 187 EVRV 189

[Close]

BLAST of CSPI05G06950 vs. TAIR 10
Match: AT3G58990.1 (isopropylmalate isomerase 1 )

HSP 1 Score: 89.4 bits (220), Expect = 1.4e-18
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0


Query: 1   MKTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLES 60
           MK+KYS+II GDNFGCGSSREH PV LG + A AVVAESYA IF   N +AT EI+PLES
Sbjct: 130 MKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESYARIFF-RNCVATGEIFPLES 189

Query: 61  ETRI 65
           E RI
Sbjct: 190 EVRI 192

[Close]

BLAST of CSPI05G06950 vs. TAIR 10
Match: AT2G43100.1 (isopropylmalate isomerase 2 )

HSP 1 Score: 85.5 bits (210), Expect = 2.0e-17
Identity = 42/63 (66.67%), Postives = 51/63 (80.95%), Query Frame = 0


Query: 2   KTKYSIIIVGDNFGCGSSREHVPVALGVSDAVAVVAESYAHIFLTANSMATREIYPLESE 61
           ++KYSIII G+NFGCGSSREH PV LG + A A+VAESYA IF   NS+AT E++PLESE
Sbjct: 134 RSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFF-RNSVATGEVFPLESE 193

Query: 62  TRI 65
            R+
Sbjct: 194 VRV 195

[Close]

BLAST of CSPI05G06950 vs. TAIR 10
Match: AT2G43090.2 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 0


Query: 28  GVSDAVAVVAESYAHIFLTANSMATREIYPLESETRI 65
           G + A AVVA+SYA IF   NS+AT E+YPL+SE R+
Sbjct: 125 GAAGAKAVVAQSYARIFF-RNSVATGEVYPLDSEVRV 160

The following BLAST results are available for this feature:

BLAST of CSPI05G06950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZW85	1.2e-17	70.31	3-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9LYT7	2.0e-17	71.88	3-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9ZW84	2.9e-16	66.67	3-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q7M887	4.2e-07	59.52	3-isopropylmalate dehydratase small subunit OS=Wolinella succinogenes (strain AT...	[more]
A8ETJ8	9.2e-07	60.98	3-isopropylmalate dehydratase small subunit OS=Arcobacter butzleri (strain RM401...	[more]

BLAST of CSPI05G06950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPA8	5.6e-31	96.05	Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G162650...	[more]
A0A5A7T900	6.4e-19	84.38	3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo var. makuwa O...	[more]
A0A0A0KPB2	6.4e-19	84.38	Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G165170...	[more]
A0A1S3AT32	6.4e-19	84.38	3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1ID74	3.2e-18	82.81	3-isopropylmalate dehydratase small subunit 3-like OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of CSPI05G06950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152394.1	1.3e-18	84.38	3-isopropylmalate dehydratase small subunit 3 [Cucumis sativus] >KGN50272.1 hypo...	[more]
XP_038876127.1	1.3e-18	84.38	3-isopropylmalate dehydratase small subunit 1-like [Benincasa hispida]	[more]
XP_008437054.1	1.3e-18	84.38	PREDICTED: 3-isopropylmalate dehydratase small subunit 3-like [Cucumis melo] >KA...	[more]
XP_022922281.1	6.6e-18	82.81	3-isopropylmalate dehydratase small subunit 3-like [Cucurbita moschata]	[more]
XP_022972989.1	6.6e-18	82.81	3-isopropylmalate dehydratase small subunit 3-like [Cucurbita maxima]	[more]

BLAST of CSPI05G06950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43090.1	8.3e-19	70.31	Aconitase/3-isopropylmalate dehydratase protein	[more]
AT3G58990.1	1.4e-18	71.88	isopropylmalate isomerase 1	[more]
AT2G43100.1	2.0e-17	66.67	isopropylmalate isomerase 2	[more]
AT2G43090.2	2.0e-04	59.46	Aconitase/3-isopropylmalate dehydratase protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000573	Aconitase A/isopropylmalate dehydratase small subunit, swivel domain	PFAM	PF00694	Aconitase_C	coord: 4..50 e-value: 5.9E-12 score: 46.1
IPR015928	Aconitase/3-isopropylmalate dehydratase, swivel	GENE3D	3.20.19.10	Aconitase, domain 4	coord: 1..76 e-value: 1.5E-15 score: 59.2
None	No IPR available	PANTHER	PTHR43345:SF2	3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED	coord: 1..68
None	No IPR available	PANTHER	PTHR43345	3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED	coord: 1..68
None	No IPR available	SUPERFAMILY	52016	LeuD/IlvD-like	coord: 4..46

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G06950.1	CSPI05G06950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043436	oxoacid metabolic process
molecular_function	GO:0016836	hydro-lyase activity