CSPI05G04640 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G04640
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Chr5: 4478940 .. 4481637 (-)
RNA-Seq Expression	CSPI05G04640
Synteny	CSPI05G04640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAAATGACAAATATGTTATCCACCTATTTCCTTCTCTCTCATCCTGCCTTATAAATTACAGTCCGAACCCAAACCCAACCCCCCCCTTCTCTTAGCTCAAAATTGACAGATGAACAACGGCGTGACCTGCCATTTCTGACATCGATCGATATCCCCTCCATTGAAGAAACAAAAGGAAAACAATAACATGCTTCTCTCCTTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCTACCGCCGAACACGTCACCGCCTCCCTCCCTCCTTCCTTCACCGCCATCATCACCGGTAACGTAACTAACTTCAACTTTAACTTCTTTCGTTATCATGAATAAAAGGGATTTTTAACCATCTTTTTTACTTCAGGTGCCACATCAGGGATTGGAGCTGAGACGGCTCGGATTCTTGCAAAACGTGGGGCTCGTTTAATCCTCCCGTCGCGGAACATTAAAGCCGCTGAAGAAACCAAGGCTCGGATTGAGTCGGAATGCTCTGATTCCGAGATTATTGTTATGTCACTCGATTTGAGCTCTTTGTCTTCTGTTAGAACCTTTGTTTCTCAATTTGAATCCCTTAATCTCCCGCTTAATCTCCTCATGTAAATTCCCTAATCACACCTTCGCTTTTTTTTGCAAAAAAATTGCTAATTTGGTCGATCTCTATGGTATTGAACTCTTTTTTCTTTTTTTGTTTGGGTTTTAGTAACAATGCGGGGAAATTCTCGCACGAGCATGCAATAACGGAGGATGGGATAGAGATGACATTCGCTACTAATTATTTGGGTAACTTTTTTTTTTTGTTTGAATGATGATAATTTATTTATGAGATTTTTATAGGGAGAAAAAGGGGATATATTGATTAACGTAATGAAATAATTTCAGGGCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGTGGAGACGGCGAAAGTGACAGGGGTTGAGGGCCGGATTGTGAACGTTTCGTCGAGTATTCATGGCTGGTTTTCCGGCGATATCCTCAAATATCTCGGCCAGATTAGCCGGAATAAAAGGTATGTATTTAATATTGAAAGCTTTTTTATTTTTTATTCTTTTTGTACTGATCTTATTTTTAAAAACAAAAGTTCATTTTCATTCCATATATTTCCAAAACGATTTTCTAGAGGGTGAAGGTTTTATCTTTGTACTTTTTTTAACTAAGAAAACAATAGCTATCTTTCCTTCAGTTACTATAGTCGCAAAAGTTACTATTTGGTTCTTTCTTAGTTAAAATAGTAAATAAATGATTGATTATTTTAGTGGATTTTCTTTGGAGGTATTATTATGAATAAAAGAAAAACTATTTTCACATGTAAAACAACTTAAATAAATTTTTGCTGTATTTAAAAGAAATCATTTGAAATAAAGTATTTAGTTTCTTTATGAAATTTGAACCATTTAAAAGTGATTATTTTGATCTTTAAAATTGTAATAAAATGGTCACTTTATTACAATTCATGGAGAAAGTTTTTTAATTAATTTTTTTAAAAATTCACTCCATATATTTATTACAATCAACCAAACTCTTTAATCACAAATGATCATAATAATCTTAAAGAAAAAAAAAACTAAATGAATGTTTTTGTATAAAATAGAGTAATAAAAGAGAGGATGAGATTTGACTTTATGAGAAGTAATGTATAATTAAATGATCTAATACAGTTAATGTGGGTGGGATTCCCAGCAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAACTTGCCAACGTTCTTCATACCCACGAACTTGCTCGCCGATTCAAGGTTCTTTAATCTTCATATTATTATATCAACTCTCTCCTTTTCTTTTTCTTTTTAATCTAATCTTTTTTTTAATCAAATATTTATTTTATTTGATTTAGCAAATGGGAGCTAATGTTACGGTGAACTGCGTCCATCCCGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGCTTCATCACAGGTATATGAACTTTCTTTTTTTTCTTTCTTTTAATGAATACGCTACATAGTACATTTAAATTTTAATCTATTGTTTTTAAAACCATGTAGATTTGGTGTTCTTCATGGCTTCCAAGCTCTTGAAGACAATACCTCAGGTATACATACAACCCAAACTTCCATTTTTCTATTTACTGTGATTATTAGTTTACATTTTCTGTTTGTAATTTCCCGAGGAAAGAAAGAAGAAAAGGCTATATAGTAAAAGAACTGACACAAAGGCCATTTCTGATTTTCCTACCTTCCACAGGCGGCGGCAACGACATGCTACGTGGCGACAAATCCGAGGCTACGCCACGTCACCGGCAAGTATTTCGTCGACTGCAACGAATCGTCACCGTCCAAGCTGGCTGGCAGCCCGTCGGAGGCCGCCCGTCTGTGGTCCGCTTCCGAGTTCATGGTCAACGCAAACTCAAAGCTTCTTTTCGATCCAAACAATGCATTGGAATAATTTAATAACTCCAAAAGAAAGAGAGAAAAAAAAGAAAGAAAAAGAAATTGCTTGTTTTGTTTGGAGAGAGAGAATCTTAGTCCAAAAATCAATTATAAAAATATATTAATAGATGGTAGGTAGACAAAAAGCAAGAAGCTAGCTAGGTAGTGGGGGGGGAAGGAATATATATATAGTTTTATTTTTTCTTTTTTAGAATATGGTCATGG

mRNA sequence

CTAAAATGACAAATATGTTATCCACCTATTTCCTTCTCTCTCATCCTGCCTTATAAATTACAGTCCGAACCCAAACCCAACCCCCCCCTTCTCTTAGCTCAAAATTGACAGATGAACAACGGCGTGACCTGCCATTTCTGACATCGATCGATATCCCCTCCATTGAAGAAACAAAAGGAAAACAATAACATGCTTCTCTCCTTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCTACCGCCGAACACGTCACCGCCTCCCTCCCTCCTTCCTTCACCGCCATCATCACCGGTGCCACATCAGGGATTGGAGCTGAGACGGCTCGGATTCTTGCAAAACGTGGGGCTCGTTTAATCCTCCCGTCGCGGAACATTAAAGCCGCTGAAGAAACCAAGGCTCGGATTGAGTCGGAATGCTCTGATTCCGAGATTATTGTTATGTCACTCGATTTGAGCTCTTTGTCTTCTGTTAGAACCTTTGTTTCTCAATTTGAATCCCTTAATCTCCCGCTTAATCTCCTCATTAACAATGCGGGGAAATTCTCGCACGAGCATGCAATAACGGAGGATGGGATAGAGATGACATTCGCTACTAATTATTTGGGGCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGTGGAGACGGCGAAAGTGACAGGGGTTGAGGGCCGGATTGTGAACGTTTCGTCGAGTATTCATGGCTGGTTTTCCGGCGATATCCTCAAATATCTCGGCCAGATTAGCCGGAATAAAAGCAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAACTTGCCAACGTTCTTCATACCCACGAACTTGCTCGCCGATTCAAGCAAATGGGAGCTAATGTTACGGTGAACTGCGTCCATCCCGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGCTTCATCACAGATTTGGTGTTCTTCATGGCTTCCAAGCTCTTGAAGACAATACCTCAGGCGGCGGCAACGACATGCTACGTGGCGACAAATCCGAGGCTACGCCACGTCACCGGCAAGTATTTCGTCGACTGCAACGAATCGTCACCGTCCAAGCTGGCTGGCAGCCCGTCGGAGGCCGCCCGTCTGTGGTCCGCTTCCGAGTTCATGGTCAACGCAAACTCAAAGCTTCTTTTCGATCCAAACAATGCATTGGAATAATTTAATAACTCCAAAAGAAAGAGAGAAAAAAAAGAAAGAAAAAGAAATTGCTTGTTTTGTTTGGAGAGAGAGAATCTTAGTCCAAAAATCAATTATAAAAATATATTAATAGATGGTAGGTAGACAAAAAGCAAGAAGCTAGCTAGGTAGTGGGGGGGGAAGGAATATATATATAGTTTTATTTTTTCTTTTTTAGAATATGGTCATGG

Coding sequence (CDS)

ATGCTTCTCTCCTTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCTACCGCCGAACACGTCACCGCCTCCCTCCCTCCTTCCTTCACCGCCATCATCACCGGTGCCACATCAGGGATTGGAGCTGAGACGGCTCGGATTCTTGCAAAACGTGGGGCTCGTTTAATCCTCCCGTCGCGGAACATTAAAGCCGCTGAAGAAACCAAGGCTCGGATTGAGTCGGAATGCTCTGATTCCGAGATTATTGTTATGTCACTCGATTTGAGCTCTTTGTCTTCTGTTAGAACCTTTGTTTCTCAATTTGAATCCCTTAATCTCCCGCTTAATCTCCTCATTAACAATGCGGGGAAATTCTCGCACGAGCATGCAATAACGGAGGATGGGATAGAGATGACATTCGCTACTAATTATTTGGGGCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGTGGAGACGGCGAAAGTGACAGGGGTTGAGGGCCGGATTGTGAACGTTTCGTCGAGTATTCATGGCTGGTTTTCCGGCGATATCCTCAAATATCTCGGCCAGATTAGCCGGAATAAAAGCAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAACTTGCCAACGTTCTTCATACCCACGAACTTGCTCGCCGATTCAAGCAAATGGGAGCTAATGTTACGGTGAACTGCGTCCATCCCGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGCTTCATCACAGATTTGGTGTTCTTCATGGCTTCCAAGCTCTTGAAGACAATACCTCAGGCGGCGGCAACGACATGCTACGTGGCGACAAATCCGAGGCTACGCCACGTCACCGGCAAGTATTTCGTCGACTGCAACGAATCGTCACCGTCCAAGCTGGCTGGCAGCCCGTCGGAGGCCGCCCGTCTGTGGTCCGCTTCCGAGTTCATGGTCAACGCAAACTCAAAGCTTCTTTTCGATCCAAACAATGCATTGGAATAA

Protein sequence

MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE*

Homology

[Close]

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 153/321 (47.66%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 5   FNYLSGSPGPSGFGSKSTAEHVTASLPPS-FTAIITGATSGIGAETARILAKRGARLILP 64
           +  ++G  G SG+GS STAE VT S+     TAIITG TSGIG E AR+L  RGA +I+ 
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 63

Query: 65  SRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKF 124
           SRN KAA ++K  I     ++ I  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG  
Sbjct: 64  SRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 123

Query: 125 SHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH--GWFS 184
                ++EDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   +  
Sbjct: 124 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 183

Query: 185 GDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVR 244
           G +  Y+     N    Y   +AY  SKLAN+LH++ L+R+ ++ G N+T+N VHPG++ 
Sbjct: 184 GIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLIT 243

Query: 245 TRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSK 304
           T L R   G    ++  M+  L K IPQ AATTCYVA +P L+ VTGKYF DCN ++PS 
Sbjct: 244 TNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSN 303

Query: 305 LAGSPSEAARLWSASEFMVNA 323
            A   + A +LW  S  +V +
Sbjct: 304 FAIDTTLADKLWDFSIKLVES 318

[Close]

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-70
Identity = 152/321 (47.35%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 5   FNYLSGSPGPSGFGSKSTAEHVTASLPPS-FTAIITGATSGIGAETARILAKRGARLILP 64
           +  ++G  G SG+GS STAE VT S+     TAIITG TSGIG E AR+L  RGA +I+ 
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 63

Query: 65  SRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKF 124
           +RN KAA ++K  I     ++ I  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG  
Sbjct: 64  ARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 123

Query: 125 SHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH--GWFS 184
                ++EDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   +  
Sbjct: 124 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 183

Query: 185 GDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVR 244
           G +  Y+     N    Y   +AY  SKLAN+LH++ L+R+ ++ G N+T+N VHPG++ 
Sbjct: 184 GIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLIT 243

Query: 245 TRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSK 304
           T L R   G    ++  M+  L K IPQ AATTCYVA +P L+ VTGKYF DCN ++PS 
Sbjct: 244 TNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSN 303

Query: 305 LAGSPSEAARLWSASEFMVNA 323
            A   + A +LW  S  +V++
Sbjct: 304 FAIDTTLADKLWDFSIKLVDS 318

[Close]

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 139/316 (43.99%), Postives = 200/316 (63.29%), Query Frame = 0


Query: 10  GSPGPSGFGSKSTAEHVTASLP-PSFTAIITGATSGIGAETARILAKRGARLILPSRNIK 69
           GS G SGF S+STAE VT  +     TAI+TGA+SGIG ETAR+L+ RG  +++  RN  
Sbjct: 5   GSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVRNTD 64

Query: 70  AAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKFSHEHA 129
           +  + K  I  +   +++ VM LDLSS+ SVR F S+++S  LPLNLLINNAG  +    
Sbjct: 65  SGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPFM 124

Query: 130 ITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGDILKYL 189
           +++D IE+ FATN+LGHFLLTKLL++ M  T++ +  EGRIVN+SS  H +   + +++ 
Sbjct: 125 LSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRF- 184

Query: 190 GQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVRTRLTRDR 249
                N   +Y + RAY  SKL NVLH +EL ++ K+ G N+T N +HPG + T L R  
Sbjct: 185 --DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYF 244

Query: 250 EGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLAGSPSE 309
             ++   V  +A  +LK++PQ AATTCYVA NP++  V+G+YF D N + P  L      
Sbjct: 245 NPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTEL 304

Query: 310 AARLWSASEFMVNANS 325
           A ++W  S  + ++ S
Sbjct: 305 AKKVWDFSTKLTDSQS 317

[Close]

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 132/309 (42.72%), Postives = 190/309 (61.49%), Query Frame = 0


Query: 9   SGSPGPSGFGSKSTAEHVTASLPPS-FTAIITGATSGIGAETARILAKRGARLILPSRNI 68
           S   G SGF   STAE VT  +  +  TAI+TGA+SGIGAET R+LA RGA +I+  RN+
Sbjct: 5   SSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNM 64

Query: 69  KAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKFSHEH 128
            AA++ K  I  +   +++  + LDLSSL SV+ F S+F S   PLN+LINNAG  +   
Sbjct: 65  VAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPF 124

Query: 129 AITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGDILKY 188
            +++D IE+ FATN++GHFLLT LL++ M +T + +  EGRIVNV+S  H +   + +++
Sbjct: 125 KLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF 184

Query: 189 LGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVRTRLTRD 248
                 N   +Y+  RAY  SKLANVLH ++L +  K+ G N+T N +HPG + T L R 
Sbjct: 185 ---DKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRH 244

Query: 249 REGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLAGSPS 308
               +  L+  +   +LK + Q AATTCYVA +P+++ V+G+YF D N    +       
Sbjct: 245 NSA-VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVD 304

Query: 309 EAARLWSAS 317
            A +LW  S
Sbjct: 305 LAKKLWDFS 309

[Close]

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.2e-43
Identity = 114/283 (40.28%), Postives = 162/283 (57.24%), Query Frame = 0


Query: 37  IITGATSGIGAETARILAKRGARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSL 96
           +ITGA +GIG ETAR LA+RGAR+ +  R++   E   + I+++  +S+++V  LDLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 97  SSVRTFVSQFESLNLPLNLLINNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKM 156
            S+R F   F +    L++LINNAG     ++ T DG E   A N+LGHFLLT LL+ ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 157 VETAKVTGVEGRIVNVSSSIHGWFSGDILKYLGQI---SRNKSRNYDATRAYALSKLANV 216
            E+A       R+VN+SS  H         +LG+I        + Y+   AY  SKLANV
Sbjct: 163 KESA-----PARVVNLSSVAH---------HLGKIRFHDLQGDKYYNLGFAYCHSKLANV 222

Query: 217 LHTHELARRFKQMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAAT 276
           L T ELA+R K  G  VT   VHPGIVR++L   R  F+  L++ + S  LKT  + A T
Sbjct: 223 LFTRELAKRLK--GTGVTTYAVHPGIVRSKLV--RHSFLLCLLWRLFSPFLKTTWEGAQT 282

Query: 277 TCYVATNPRLRHVTGKYFVDCNESSPSKLAGSPSEAARLWSAS 317
           + + A    L  ++GKYF DC ++  S  A +   A RLW+ S
Sbjct: 283 SLHCALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVS 307

[Close]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL
Match: A0A0A0KKE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.2e-181
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60
           MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60

Query: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120
           ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA
Sbjct: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120

Query: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180
           GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF
Sbjct: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180

Query: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240
           SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV
Sbjct: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240

Query: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300
           RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS
Sbjct: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300

Query: 301 KLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           KLAGSPSEAARLWSASE MVNANSKLLFDPNNALE
Sbjct: 301 KLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 335

[Close]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL
Match: A0A5D3C462 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001850 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.0e-177
Identity = 328/335 (97.91%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60
           MLLSFNYLSGSPGPSGFGSKSTAEHVT+SLPPSFTAIITGATSGIGAETARILAKRGARL
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTSSLPPSFTAIITGATSGIGAETARILAKRGARL 60

Query: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120
           ILPSRN+KAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA
Sbjct: 61  ILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120

Query: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180
           GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH WF
Sbjct: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHSWF 180

Query: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240
           SGDILKYLGQISRNK RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV
Sbjct: 181 SGDILKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240

Query: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300
           RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS
Sbjct: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300

Query: 301 KLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           KLA SPSEAARLWSASE MVNA+SKLLFDPNNALE
Sbjct: 301 KLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

[Close]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL
Match: A0A6J1JZY1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491218 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.3e-160
Identity = 298/338 (88.17%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP---PSFTAIITGATSGIGAETARILAKRG 60
           ML SF YL+GSPGPSGFGSKSTAE VTAS P   PS TAIITGATSGIGAETAR+LAKRG
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASSPLPLPSLTAIITGATSGIGAETARVLAKRG 60

Query: 61  ARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLI 120
            RLILPSRN+KAAEETK+RI SECSDS+IIVM LDLSSLSSV  FV QF+SLNLPLNLLI
Sbjct: 61  VRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLLI 120

Query: 121 NNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH 180
           NNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVN MVETAK TGVEGRIVNVSSSIH
Sbjct: 121 NNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSIH 180

Query: 181 GWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240
           GWFSGD++KYLGQISRNKSRNYDATRAYALSKLANVLHTHELARR KQM ANVTVNCVHP
Sbjct: 181 GWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAT+PRL HVTGKYF DCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           SPSKLA SP+ AARLWS SE MVN+ SKL+FDPNN LE
Sbjct: 301 SPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 338

[Close]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL
Match: A0A6J1HY34 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 295/337 (87.54%), Postives = 314/337 (93.18%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASL--PPSFTAIITGATSGIGAETARILAKRGA 60
           ML S  YL+GSPGPSGFGSKS+AEHVTAS    PS TAIITGATSGIGAETAR+LAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLIN 120
           RLILPSRN+KAAEE KARIE+ECS SEII M+LDLSSL+SVRTFV+QFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHG 180
           NAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVN MVETAK TG++GRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPG 240
           WFSGDI+KYLGQISRNK RNYDATRAYALSKLANVLHTHELARR K+MGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESS 300
           IVRTRLTRDREGFITDLVFF+ SKLLKTIPQAAATTCYVA NPRL +VTGKYF DCNE+S
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           PSKLA S +EAARLWSASE MVN NSKL+F+PNNALE
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 337

[Close]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL
Match: A0A6J1H1D2 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459194 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 296/338 (87.57%), Postives = 310/338 (91.72%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP---PSFTAIITGATSGIGAETARILAKRG 60
           ML SF YL+GSPGPSGFGSKSTAE VTAS P   PS TAIITGATSGIGAETAR+LAKR 
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASSPLPLPSLTAIITGATSGIGAETARVLAKRR 60

Query: 61  ARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLI 120
            RLILPSRN+KAAEETKARI SECSDS+IIVM LDLSSLSSV  FV QF+SLNLPLNLLI
Sbjct: 61  VRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLLI 120

Query: 121 NNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH 180
           NNAGKFSH+HAI+EDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSIH
Sbjct: 121 NNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSIH 180

Query: 181 GWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240
           GWFSGD++KYLGQISRNKSRNYDATRAYALSKLANVLHTHELARR KQM ANV+VNCVHP
Sbjct: 181 GWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAT+PRL HVTGKYF DCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           SPSKLA SP+ AARLWSASE MVN  SKL+FDPNN LE
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 338

[Close]

BLAST of CSPI05G04640 vs. NCBI nr
Match: XP_011654698.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KGN50018.1 hypothetical protein Csa_000365 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60
           MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60

Query: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120
           ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA
Sbjct: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120

Query: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180
           GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF
Sbjct: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180

Query: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240
           SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV
Sbjct: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240

Query: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300
           RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS
Sbjct: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300

Query: 301 KLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           KLAGSPSEAARLWSASE MVNANSKLLFDPNNALE
Sbjct: 301 KLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 335

[Close]

BLAST of CSPI05G04640 vs. NCBI nr
Match: XP_004143954.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 333/335 (99.40%), Postives = 333/335 (99.40%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60
           MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60

Query: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120
           ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA
Sbjct: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120

Query: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180
           GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF
Sbjct: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180

Query: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240
           SGDILKYLGQISRNK RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV
Sbjct: 181 SGDILKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240

Query: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300
           RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS
Sbjct: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300

Query: 301 KLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           KLAGSPSEAARLWSASE MVNANSKLLFDPNNALE
Sbjct: 301 KLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 334

[Close]

BLAST of CSPI05G04640 vs. NCBI nr
Match: TYK06084.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 630.9 bits (1626), Expect = 6.1e-177
Identity = 328/335 (97.91%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLPPSFTAIITGATSGIGAETARILAKRGARL 60
           MLLSFNYLSGSPGPSGFGSKSTAEHVT+SLPPSFTAIITGATSGIGAETARILAKRGARL
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTSSLPPSFTAIITGATSGIGAETARILAKRGARL 60

Query: 61  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120
           ILPSRN+KAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA
Sbjct: 61  ILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 120

Query: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 180
           GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH WF
Sbjct: 121 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHSWF 180

Query: 181 SGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240
           SGDILKYLGQISRNK RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV
Sbjct: 181 SGDILKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIV 240

Query: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300
           RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS
Sbjct: 241 RTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPS 300

Query: 301 KLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           KLA SPSEAARLWSASE MVNA+SKLLFDPNNALE
Sbjct: 301 KLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

[Close]

BLAST of CSPI05G04640 vs. NCBI nr
Match: XP_038875365.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 615.1 bits (1585), Expect = 3.5e-172
Identity = 317/338 (93.79%), Postives = 326/338 (96.45%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP---PSFTAIITGATSGIGAETARILAKRG 60
           MLLS NYLSGSPGPSGFGSKSTAEHVT+S P   PSFTAIITGATSGIGAETAR+LAKRG
Sbjct: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSAPFLLPSFTAIITGATSGIGAETARVLAKRG 60

Query: 61  ARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLI 120
           ARLILPSRN+KAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFV+QFESLNLPLNLLI
Sbjct: 61  ARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLI 120

Query: 121 NNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH 180
           NNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKMVETAK TGVEGRIVNVSSSIH
Sbjct: 121 NNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAKATGVEGRIVNVSSSIH 180

Query: 181 GWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240
           GWFSGDI+KYLGQISRNKSRNYDATRAYALSKLANVLHTHEL RR KQMGANVTVNCVHP
Sbjct: 181 GWFSGDIIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELGRRLKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRL HVTGKYF DCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLSHVTGKYFADCNES 300

Query: 301 SPSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           SPSKLA SP+EAARLWSASE MVNANSKL+FDPNNAL+
Sbjct: 301 SPSKLAASPTEAARLWSASEIMVNANSKLVFDPNNALD 338

[Close]

BLAST of CSPI05G04640 vs. NCBI nr
Match: XP_038875366.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.5e-170
Identity = 316/338 (93.49%), Postives = 325/338 (96.15%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP---PSFTAIITGATSGIGAETARILAKRG 60
           MLLS NYLSGSPGPSGFGSKSTAEHVT+S P   PSFTAIITGATSGIGAETAR+LAKRG
Sbjct: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSAPFLLPSFTAIITGATSGIGAETARVLAKRG 60

Query: 61  ARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLI 120
           ARLILPSRN+KAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFV+QFESLNLPLNLLI
Sbjct: 61  ARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLI 120

Query: 121 NNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIH 180
           NNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKMVETAK TGVEGRIVNVSSSIH
Sbjct: 121 NNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAKATGVEGRIVNVSSSIH 180

Query: 181 GWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240
           GWFSGDI+KYLGQISRNK RNYDATRAYALSKLANVLHTHEL RR KQMGANVTVNCVHP
Sbjct: 181 GWFSGDIIKYLGQISRNK-RNYDATRAYALSKLANVLHTHELGRRLKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRL HVTGKYF DCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLSHVTGKYFADCNES 300

Query: 301 SPSKLAGSPSEAARLWSASEFMVNANSKLLFDPNNALE 336
           SPSKLA SP+EAARLWSASE MVNANSKL+FDPNNAL+
Sbjct: 301 SPSKLAASPTEAARLWSASEIMVNANSKLVFDPNNALD 337

[Close]

BLAST of CSPI05G04640 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 463.0 bits (1190), Expect = 2.0e-130
Identity = 237/331 (71.60%), Postives = 284/331 (85.80%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP-PSFTAIITGATSGIGAETARILAKRGAR 60
           M+ +  YL G+ G SGFGSKSTAE VT +    S TA+ITGATSGIGAETAR+LAKRGAR
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRSITAVITGATSGIGAETARVLAKRGAR 60

Query: 61  LILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINN 120
           LI P+RN+KAAEE K RI SE  ++EI+VM LDLSS++SVR FV+ FESL+LPLNLLINN
Sbjct: 61  LIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLINN 120

Query: 121 AGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGW 180
           AGK +HEHAI+EDGIEMTFATNYLGHFLLT LL+NKM++TA+ TGV+GRIVNV+S IHGW
Sbjct: 121 AGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHGW 180

Query: 181 FSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGI 240
           FSGD+++YL  IS+ K + +DATRAYALSKLANVLHT EL+ R +++GANVTVNCVHPG+
Sbjct: 181 FSGDLIEYLRLISQPKCQ-FDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 241 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSP 300
           VRTRLTRDREG +TDLVFF+ASKL+KT+PQAAATTCYVATNPRL +V+GKYF DCNE++P
Sbjct: 241 VRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTP 300

Query: 301 SKLAGSPSEAARLWSASEFMVNANSKLLFDP 331
           S L  + SEA +LW+ASE +V  +SK  FDP
Sbjct: 301 SGLGTNSSEATKLWAASEILVTQHSKTSFDP 330

[Close]

BLAST of CSPI05G04640 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 451.1 bits (1159), Expect = 8.1e-127
Identity = 232/322 (72.05%), Postives = 275/322 (85.40%), Query Frame = 0


Query: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP-PSFTAIITGATSGIGAETARILAKRGAR 60
           M+ +  +L GS GPSGFGS+STA+HVT +    S TAIITGATSGIGAETAR+LAKRGAR
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRSLTAIITGATSGIGAETARVLAKRGAR 60

Query: 61  LILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINN 120
           L+LP+R++K AEETKARI SE  D+EIIVM LDLSSL+SVR FV  FESLNLPLN+LINN
Sbjct: 61  LVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILINN 120

Query: 121 AGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGW 180
           AGK++H+HA++EDG+EMTFATNYLGHFLLTKLL+ KM+ETA  TGV+GRIVNV+S +H W
Sbjct: 121 AGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHSW 180

Query: 181 FSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGI 240
           FSGD+L+YL  ISRN +RNYDATRAYALSKLANVLHT EL+R   +M ANVT NCVHPGI
Sbjct: 181 FSGDMLQYLADISRN-NRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 241 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSP 300
           V+TRLTRDREG +TDLVFF+ SKLLK++PQAAATTCYVAT+PRLR+V GKYF DCNE+  
Sbjct: 241 VKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEARS 300

Query: 301 SKLAGSPSEAARLWSASEFMVN 322
           SK      +A RLW+AS+ +V+
Sbjct: 301 SKSGSCNLKAQRLWTASDLLVS 321

[Close]

BLAST of CSPI05G04640 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 171/324 (52.78%), Postives = 227/324 (70.06%), Query Frame = 0


Query: 4   SFNYLSGSPGPSGFGSKSTAEHVT--ASLPPS-FTAIITGATSGIGAETARILAKRGARL 63
           +  YL+G  GP+GFGS+STAE VT  +  P S  TAIITG TSGIGAETAR+LAKRG R+
Sbjct: 4   TLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRV 63

Query: 64  ILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNA 123
           ++  R++K AE  K RI  E  +++II+  +DLSSLSSV  F SQF S +LPLN+LINNA
Sbjct: 64  VMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILINNA 123

Query: 124 GKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWF 183
           G FS     +E+ IE+TFATN+LGH+LLT++L+ KM++TA+ +G+EGRI+N+SS IH W 
Sbjct: 124 GVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNWV 183

Query: 184 SGDIL---KYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 243
             D     K L  ISR     Y+ TRAYA SKLA +LH   L+++ K   ANVT+N VHP
Sbjct: 184 KPDCFSFPKLLHPISR-----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHP 243

Query: 244 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 303
           GIV+T + R  +G  TD +F +ASKLLK+I Q AATTCYVA +   + ++GKYF DCNE+
Sbjct: 244 GIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNET 303

Query: 304 SPSKLAGSPSEAARLWSASEFMVN 322
           + S LA     A +L + S  +++
Sbjct: 304 NCSDLANDEYVALKLCTQSRALIH 322

[Close]

BLAST of CSPI05G04640 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.0e-78
Identity = 158/322 (49.07%), Postives = 221/322 (68.63%), Query Frame = 0


Query: 5   FNYLSGSPGPSGFGSKSTAEHVTASL-PPSFTAIITGATSGIGAETARILAKRGARLILP 64
           ++ ++G  GPSGFGS STAE VT  +   + TAIITG T GIG ETAR+L+KRGA +++ 
Sbjct: 4   YSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVIG 63

Query: 65  SRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKF 124
           +RN+ AAE  K  I  + +++ + ++ LDLSS+ S++ FV +F +L+LPLNLLINNAG  
Sbjct: 64  ARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVM 123

Query: 125 SHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGD 184
              + ++EDGIE+ FATN++GHFLLT LL++ M  TAK +GVEGRI+NVSS  H +   +
Sbjct: 124 FCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQE 183

Query: 185 ILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVRTR 244
            +++    S N   +Y   RAY  SKLAN+LH +EL+R+ ++ G N+T N VHPG++ T 
Sbjct: 184 GIQF---DSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTN 243

Query: 245 LTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLA 304
           L +     +  L FF +  L K IPQ AATTCYVA +P ++ VTGKYF DCNE +PSKLA
Sbjct: 244 LFQHTALLMRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLA 303

Query: 305 GSPSEAARLWSASEFMVNANSK 326
              + A +LW  S  ++N+ SK
Sbjct: 304 RDETLAQKLWDFSVKLINSVSK 321

[Close]

BLAST of CSPI05G04640 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.2e-76
Identity = 161/322 (50.00%), Postives = 214/322 (66.46%), Query Frame = 0


Query: 5   FNYLSGSPGPSGFGSKSTAEHVTASLPPS-FTAIITGATSGIGAETARILAKRGARLILP 64
           +  ++G  G SGFGS STAE VT ++  S  TAIITG TSGIG E AR+LA RGA +I+ 
Sbjct: 4   YGVMTGKKGKSGFGSASTAEDVTQAIDASHLTAIITGGTSGIGLEAARVLAMRGAHVIIA 63

Query: 65  SRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKF 124
           +RN KAA E+K  I     ++ +  + +D+SS+ SVR+FV QF +LN+PLN+LINNAG  
Sbjct: 64  ARNPKAANESKEMILQMNPNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINNAGVM 123

Query: 125 SHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGD 184
                +TEDGIE  FATN++GHFLLT LL++KM  TA+ +GV+GRIVN+SS  H +   +
Sbjct: 124 FCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTYTYSE 183

Query: 185 ILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPGIVRTR 244
            +K+ G    N    Y   RAY  SKL+N+LH++ L+RR ++ G N+T+N VHPG+V T 
Sbjct: 184 GIKFQG---INDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTN 243

Query: 245 LTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLA 304
           L R   GF   +   M     K IPQ AATTCYVA +P L  VTGKYF DCN  +PSK A
Sbjct: 244 LFR-YSGFSMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIVAPSKFA 303

Query: 305 GSPSEAARLWSASEFMVNANSK 326
            + S A +LW  S F++++ SK
Sbjct: 304 TNNSLADKLWDFSVFLIDSISK 321

The following BLAST results are available for this feature:

BLAST of CSPI05G04640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.4e-70	47.66	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	3.0e-70	47.35	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	1.1e-64	43.99	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	1.2e-58	42.72	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
P59837	9.2e-43	40.28	Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1	[more]

BLAST of CSPI05G04640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKE2	1.2e-181	99.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1	[more]
A0A5D3C462	3.0e-177	97.91	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1JZY1	7.3e-160	88.17	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A6J1HY34	1.2e-159	87.54	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A6J1H1D2	1.4e-158	87.57	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]

BLAST of CSPI05G04640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654698.1	2.4e-181	99.70	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]
XP_004143954.1	1.7e-179	99.40	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus]	[more]
TYK06084.1	6.1e-177	97.91	short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa]	[more]
XP_038875365.1	3.5e-172	93.79	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa his...	[more]
XP_038875366.1	2.5e-170	93.49	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]

BLAST of CSPI05G04640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	2.0e-130	71.60	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	8.1e-127	72.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.1e-83	52.78	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	9.0e-78	49.07	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	2.2e-76	50.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 117..136 score: 28.38 coord: 23..34 score: 42.05 coord: 73..89 score: 28.9 coord: 140..157 score: 37.54
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 117..136 score: 29.93 coord: 79..87 score: 54.94 coord: 23..34 score: 44.39
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 2..87 e-value: 1.2E-11 score: 44.4
None	No IPR available	GENE3D	3.40.50.720		coord: 1..237 e-value: 2.0E-54 score: 186.6
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 1..238
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 1..238
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 2..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G04640.1	CSPI05G04640.1	mRNA
CSPI05G04640.2	CSPI05G04640.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity