CSPI05G03170 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G03170
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr5: 3505413 .. 3510108 (-)
RNA-Seq ExpressionCSPI05G03170
SyntenyCSPI05G03170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATAAATTGAAATAATGGGAGAGGCAAGAAGGTAGCAAGACATCCCCACATAACACACACGTATTCATACACACATTAAAGTAAAATATTTTTCCTTTTTTTCTCTTTTTCAAAGAATGAGTAGAAAGTTTTAGAAGCAAAACAAAAGGACCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATAATAATCATCTTCATCCTTGCAAGTATATATGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTATCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAAGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTAAGAATTAACTATACCCTTTTTTTAAATACTCTTAGGAAATCAACTCTCAGCTCAGTTATTGTTTATTTTGGCATAACTATACTTTTCTCTAAAATATTCTCATATACATCTTTTTTACTATTTAAATTATCCCTGCACTCTAAACTATCATACTTTTAACACGTGTCGACTTATGAGTGGACACTAGTGTTTAGTCAGAGATACACATAGTAATTATTTTTCTAAAAGTATAGTGTTTCAATGTCTACAAGAGACTATATATATATATACATATATTTGCCCTTTGATTAATACATTTTTGAAAGCAATGTAATAGGTGGTTTTAAAAACTTTAGTTTGAAGAAGATAGCTAGAAATCTAGGGGTATAGTTAGTTGGATTGTTAGAGAGTAGTGGATGATGATTTTCTTTATAATTAATTATTAAGTTAATTTTCTTAATCTTTTTTACCTTTCAAAATTCGAATGATTATTAGTGAAACTTCTTTTTCTTGGGAAAGTGGAGAAAGAAAATGGTTGTGATTAAGCCATAATTAACCAAGATTAATCAAAAGGAAGCAGGGAAAGAAGGAAAAAGAAAAGTTTTATTGACTTCAGTGTCATAATCATGCAAAGCTGTTACTTTACCAATTCCTAAAGTCCGTAGTTTTTTTTTAATATCATGATAATAATTATACAATCAAAACAATATTTTATATGTCCCCTCACAATAATTAATTTAAGTCCTTTTCAATTTAGTTGCTTTCATAATAATAACTCTTAATAACAACCAAAAATTTAAAATCAATAAAATTCAACACATGAATAAATAAAATAACCCATCATCAGAATAATTATCGGTATATATATATTTAAAGGGAAACATTTTTAGGAGAATTTATTTATTTAATTAATTAAAATTAAAATTAGGATGTTTTGATAATAATAAATTGAGAAATGGTGTGGAGTAATAGCAGGGAGATAAATGTAAATTTGTAGGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATACTCTCTCACCCTCCTTTCCCCTTTTCTTTTCTTTTCATTTTTATTCCTTTTTACTCAAAATTCTCTCCATACATAATTAATTAAAATAGCGTTAAAAAATCTTAAATTGTTTTTTTTTGGAAAAAGTTAATAACAAATTAGTAGTATAAGCTTAATTTAAAATTAATTAAATAATTAAATTGATTTTTGAATAGAAATGAAGGCTTTTAATTCTAACACACCGGATATCTAGGGCTTGGAATTATGGTCGTCCGGCCTTAGTACTAAAAGTGGCCATACTATATCACCTTTTTCTTCTTAAACTTTCTTTTTGTCTATTCAATTTTCAAAATCATATCATCCCCAAATTAAGAAGTAGATTTAAAAATTTTCATATAAAATATATGAATATCAAAATGGACCCATATGCATGTAGATTTGTTAGAATATTAGATATTTAACGAGTATTTAATACGTACAATAATACAGTAAGTTTAAATCCTTGTGGTAGTTTTGAGAGTTTAGAGAACGAAATAATATTAATTAAAAAAAGAATTTCTATAGTAATAATAATTATTATTATCATAATTTTTGTTTAAAAAAATTGGGGTTATATTTCAAGATAGTGACAAATTAAGCTACTTTGGTAGCATTTAATGGGCTATAGAAATAATTGTTTGATTTATATCCAAATAATCATAAGAGAAACAGTAGATTCCAGCTGTGTTTTGTCAAGGAGTCTTCATCATTAATTATTTTTGTAGGAAAAAACACACACCATTCATTTTTTTTTAATAATTATATGCTATGCTAGTGTATACGTTTACTACTAAACTCAGGCTTACAAATTCAATCTTTGACGTAACATTTTCACATGGCATATTATTAAATATATATCATCATAAATATTCTTTTACTACTAAGGGTATTATAAAACTTTGATCATCTTTCAAAAAATAATAAAATATCATATGATAGTCGTAATTAGGCAAGTTGTATTAAAGGGTTATTATTATTATTATTATTCATATTATAAGAAGATTCTATAGTAGGTGTTTTAAAAATCTTTTTGTTATCTCTATAAGAAAGATGATGTGTCTTTCATTATTATATAATATATTGTGCCTATCATAATTAATTTGTAAAGTATTCCACTCTAATAAATTGTTATAATATTAATAATTTTTGGAAGAAGAAGAAGAAGAAGATGAGAATAATAATAAAAAGAATGAAATGGAATGGAATATCCCAATTGTTCATGATGATTTAGAAAAGTCATAGTAGTATTATTCCCATGTTTCTTGCATCATGCTTACTTCCACAAGTTGGTAAAAAGTTTTCTTTTTTCAAATGGGAATCTAATATGGTCCAAATAGAGATGCCAAAAGCTTACTCTCCATGGAGATAAGGATAGATGTTAGAAATTAATTATTGACTTTTATTTCAAAAGTTCTCCATCTCTCATCACATAATCTATGGTTCATGATTGTGTCATGAGCACTTAAACATTAAACATTATTAAGATTTAAATGGTATGTCAATGTTTAATATGTTAATTTTTTTTTTTTTTTTTTTGTTGTAAACAGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAACACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCATGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTACGAGTATATGAATCCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATATATATATATATATATATAT

mRNA sequence

CATAAATTGAAATAATGGGAGAGGCAAGAAGGTAGCAAGACATCCCCACATAACACACACGTATTCATACACACATTAAAGTAAAATATTTTTCCTTTTTTTCTCTTTTTCAAAGAATGAGTAGAAAGTTTTAGAAGCAAAACAAAAGGACCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATAATAATCATCTTCATCCTTGCAAGTATATATGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTATCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAAGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAACACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCATGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTACGAGTATATGAATCCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATATATATATATATATATATAT

Coding sequence (CDS)

ATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTATCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAAGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAACACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCATGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTACGAGTATATGAATCCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAA

Protein sequence

MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLSTN*
Homology
[Close]
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.3e-199
Identity = 365/596 (61.24%), Postives = 438/596 (73.49%), Query Frame = 0 

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
           P+     +  DP      L+++++  +I F   +L+   VV   I        S    P 
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAF-----SLSLFSKDASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA      S+ + S D S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF ++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
           + +  T          E  P WL  +DR LL  P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G   I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYG  
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYG-- 547

Query: 560 EYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 605
           EYMN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 EYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.8e-193
Identity = 346/601 (57.57%), Postives = 438/601 (72.88%), Query Frame = 0 

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R  + 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSKDASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S  A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF  V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G   I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 605
           YYG  EY+N GPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGL
Sbjct: 554 YYG--EYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 597

[Close]
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 464.2 bits (1193), Expect = 2.3e-129
Identity = 260/591 (43.99%), Postives = 363/591 (61.42%), Query Frame = 0 

Query: 37  SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISR-ACSHT 96
           + ++  +++++S    LL  A+V  +SA     +   +  P       S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSP------SSHAVLRSSCSST 72

Query: 97  LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
            YP LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGF-QDV 216
             DC+E +++ +D        L L+   K    H  D+ T +S+A+T  +TC  GF  D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESSTTSW 276
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+  T+ 
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
           A +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
           +S +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
             G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
            I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT 
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMN 576
           DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY   N
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYS--N 552

Query: 577 PGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
            G GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 463.0 bits (1190), Expect = 5.2e-129
Identity = 263/582 (45.19%), Postives = 350/582 (60.14%), Query Frame = 0 

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
           S  +   ++++ S    LL+ AS+V  A    ++   +Q     +  S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIA-ATTTNQNKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
           YP LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
            DC+E +++ +D   +++         K    H  D+ T +S+A+T   TC  GF  D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGG- 275
           D  V+  +      +  M SN+LA+       D+   E R +     ++      E  G 
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251

Query: 276 -DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
            D +G+P WLS  DRRLL    STI+AD  VA DGSG F TVA A+ AAP  S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311

Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + R
Sbjct: 312 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371

Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
           D+TF+NTAGPSKHQAVALR+ +D +  Y C++  YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431

Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
           FGNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491

Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMNPGPGAAVGQ 575
           PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y   EY+N G GA    
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTY--REYLNRGGGAGTAN 551

Query: 576 RVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
           RVKW GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 RVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.4e-128
Identity = 264/593 (44.52%), Postives = 365/593 (61.55%), Query Frame = 0 

Query: 25  RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQ 84
           +LS +      S++ ++ + L ++  + LL+ A+V+     V S   S       N+P  
Sbjct: 10  KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69

Query: 85  AISR-ACSHTLYPSLCLSSLLSFPGAQS--ADVHDLVHISLNLTLQHLTKALYSTSQIPV 144
           AI + +CS T YP LC S++ + P A        D++ +SLN+T   +    +   ++ +
Sbjct: 70  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129

Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTY 204
            + +       A  DC+E +++ +D    ++         K  S H  D+ T +SAA+T 
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189

Query: 205 HDTCTAGF-QDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV-ENRKRRRLME 264
             TC  GF  D A+  V+D +      + +M SN+LA+       D+ +      R+L+E
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIE 249

Query: 265 SSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAA 324
            ++T          +G+PAWLS  DRRLL +  S++  ++VVA DGSG FKTVA ++ AA
Sbjct: 250 ETSTV---------DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 309

Query: 325 PSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTAT 384
           P    +R II IKAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT
Sbjct: 310 PQGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 369

Query: 385 FAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFR 444
            A  G   + RD+TF+NTAGPSKHQAVALR+ AD +  Y+C+++ YQDTLYVHSNRQFF 
Sbjct: 370 VAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 429

Query: 445 ECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVA 504
            C I GT+DFIFGNA  V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I   +I A
Sbjct: 430 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGA 489

Query: 505 TGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEY 564
           T DL+  +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE++ 
Sbjct: 490 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQ- 549

Query: 565 MNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
            N G GA    RVKW G+RVITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 550 -NAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 603/606 (99.50%), Postives = 603/606 (99.50%), Query Frame = 0 

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
           ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 600
           DTLYYG  EYMN GPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS
Sbjct: 541 DTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 600

Query: 601 AGLSTN 607
           AGLSTN
Sbjct: 601 AGLSTN 604

[Close]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 580/607 (95.55%), Postives = 588/607 (96.87%), Query Frame = 0 

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 600
           LDTLYYG  EYMN GPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 LDTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 600

Query: 601 SAGLSTN 607
           SAGLSTN
Sbjct: 601 SAGLSTN 605

[Close]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.4e-291
Identity = 514/606 (84.82%), Postives = 552/606 (91.09%), Query Frame = 0 

Query: 4   NNSYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YN LH+SHS + TPA+SFR+S   +DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSHM-TSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S +  S++ HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           DTLYYG  EYMN GPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 542 DTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 601

Query: 604 AGLSTN 607
           AGLSTN
Sbjct: 602 AGLSTN 602

[Close]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.6e-288
Identity = 512/609 (84.07%), Postives = 550/609 (90.31%), Query Frame = 0 

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGN-----DPPPPSSSLRRRSRILIISFMSVLLIAAS 63
           NN+YNKLH+S S + TP  SFR+S +      PPPPSSS R   RIL+ISF S+LLI AS
Sbjct: 2   NNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITAS 61

Query: 64  VVSAAILVRSHM-TSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDL 123
            VSA +LV S + TS++ HFPHNKP+QAISRACS TLYPSLC+SSLLS PG+ SADVHDL
Sbjct: 62  TVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 121

Query: 124 VHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDA 183
           VHISLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A
Sbjct: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 181

Query: 184 SNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD 243
           +NHD+MTWLSAALTYHDTCT GF DVAD  VKD++ AKLSDLSEMISNSLAIFSGFGGGD
Sbjct: 182 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGGD 241

Query: 244 LPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGS 303
           LPVENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGS
Sbjct: 242 LPVENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGS 301

Query: 304 GKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 363
           GKFKTVAEAI AAPSSS RRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 302 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361

Query: 364 SVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 423
           SVYDKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADH VV+  N+IGYQ
Sbjct: 362 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 421

Query: 424 DTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 483
           DTLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 422 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 481

Query: 484 NTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 543
           NTGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 482 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 541

Query: 544 FALDTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGV 603
           FALDTLYYG  EYMN GP AAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGV
Sbjct: 542 FALDTLYYG--EYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGV 601

Query: 604 SFSAGLSTN 607
           SFSAGLSTN
Sbjct: 602 SFSAGLSTN 602

[Close]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.8e-280
Identity = 504/604 (83.44%), Postives = 542/604 (89.74%), Query Frame = 0 

Query: 5   NSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64
           N+YNKLH+S S +T   A+F++S    PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 2   NNYNKLHSSASGDT--PATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61

Query: 65  LVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNL 124
           L+ S  +    HFPHNKP+QAIS ACSHTLYPSLC SSLLSFPG+ SADVH LVHISLNL
Sbjct: 62  LLHSQSS----HFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121

Query: 125 TLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIMT 184
           TLQHLTKALY++SQIPV QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFS  ASNHDIMT
Sbjct: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181

Query: 185 WLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244
           WLSAALTYHDTC+ GF DV D  VKD++ A+LSDLSEMISNSLAIFSG+G G LPV+N  
Sbjct: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQN-- 241

Query: 245 RRRLMESSTTSWAAENG-GDHEG-FPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKT 304
           RRRLMES    WAAENG    EG FP W+SGKDRRLLAAP+S IQADIVVAKDGSGKFKT
Sbjct: 242 RRRLMESD-REWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 301

Query: 305 VAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 364
           VAEAIEAAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 361

Query: 365 VTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYV 424
           VTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQDTLYV
Sbjct: 362 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYV 421

Query: 425 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 484
           HSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM  QKNTITAQ+RKDPNQNTGIS
Sbjct: 422 HSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGIS 481

Query: 485 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 544
           IHACKI+AT DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT
Sbjct: 482 IHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 541

Query: 545 LYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 604
           LYYG  EYMN GPGAA GQRV WPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 LYYG--EYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAG 592

Query: 605 LSTN 607
           LS N
Sbjct: 602 LSAN 592

[Close]
BLAST of CSPI05G03170 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 603/606 (99.50%), Postives = 603/606 (99.50%), Query Frame = 0 

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
           ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 600
           DTLYYG  EYMN GPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS
Sbjct: 541 DTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 600

Query: 601 AGLSTN 607
           AGLSTN
Sbjct: 601 AGLSTN 604

[Close]
BLAST of CSPI05G03170 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 580/607 (95.55%), Postives = 588/607 (96.87%), Query Frame = 0 

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 600
           LDTLYYG  EYMN GPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 LDTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 600

Query: 601 SAGLSTN 607
           SAGLSTN
Sbjct: 601 SAGLSTN 605

[Close]
BLAST of CSPI05G03170 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 1067.0 bits (2758), Expect = 6.0e-308
Identity = 542/606 (89.44%), Postives = 567/606 (93.56%), Query Frame = 0 

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63
           NN+YNKLH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62

Query: 64  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           SAA+LVRS + S++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFS+SLFS +ASNH
Sbjct: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           +NR RRRLMES T SWAAENGGD  GFP WLS KDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 243 QNR-RRRLMESGTRSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT IILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542

Query: 544 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           DTLYYGEY   N GPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS
Sbjct: 543 DTLYYGEYS--NSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 602

Query: 604 AGLSTN 607
           AGLS N
Sbjct: 603 AGLSAN 604

[Close]
BLAST of CSPI05G03170 vs. NCBI nr
Match: XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.9e-290
Identity = 514/606 (84.82%), Postives = 552/606 (91.09%), Query Frame = 0 

Query: 4   NNSYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YN LH+SHS + TPA+SFR+S   +DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSHM-TSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S +  S++ HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           DTLYYG  EYMN GPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 542 DTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 601

Query: 604 AGLSTN 607
           AGLSTN
Sbjct: 602 AGLSTN 602

[Close]
BLAST of CSPI05G03170 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.5e-290
Identity = 514/606 (84.82%), Postives = 552/606 (91.09%), Query Frame = 0 

Query: 4   NNSYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YNKLH+S S + TPA+SFR+S   +DP PP S    R RIL+ISF+S+ LI AS VS
Sbjct: 2   NNNYNKLHSSRSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVS 61

Query: 64  AAILVRSHM-TSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S +  S++ HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEHWLAEENNGDGD-FPQWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           DTLYYG  EYMN GPGAAVGQRVKWPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 542 DTLYYG--EYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 601

Query: 604 AGLSTN 607
           AGLSTN
Sbjct: 602 AGLSTN 602

[Close]
BLAST of CSPI05G03170 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 697.6 bits (1799), Expect = 8.9e-201
Identity = 365/596 (61.24%), Postives = 438/596 (73.49%), Query Frame = 0 

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
           P+     +  DP      L+++++  +I F   +L+   VV   I        S    P 
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAF-----SLSLFSKDASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA      S+ + S D S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF ++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
           + +  T          E  P WL  +DR LL  P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G   I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYG  
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYG-- 547

Query: 560 EYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 605
           EYMN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 EYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]
BLAST of CSPI05G03170 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 677.2 bits (1746), Expect = 1.2e-194
Identity = 346/601 (57.57%), Postives = 438/601 (72.88%), Query Frame = 0 

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R  + 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSKDASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S  A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF  V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G   I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYEYMNPGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 605
           YYG  EY+N GPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGL
Sbjct: 554 YYG--EYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 597

[Close]
BLAST of CSPI05G03170 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-130
Identity = 260/591 (43.99%), Postives = 363/591 (61.42%), Query Frame = 0 

Query: 37  SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISR-ACSHT 96
           + ++  +++++S    LL  A+V  +SA     +   +  P       S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSP------SSHAVLRSSCSST 72

Query: 97  LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
            YP LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGF-QDV 216
             DC+E +++ +D        L L+   K    H  D+ T +S+A+T  +TC  GF  D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESSTTSW 276
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+  T+ 
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
           A +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
           +S +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
             G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
            I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT 
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMN 576
           DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY   N
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYS--N 552

Query: 577 PGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
            G GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]
BLAST of CSPI05G03170 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.7e-130
Identity = 263/582 (45.19%), Postives = 350/582 (60.14%), Query Frame = 0 

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
           S  +   ++++ S    LL+ AS+V  A    ++   +Q     +  S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIA-ATTTNQNKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
           YP LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
            DC+E +++ +D   +++         K    H  D+ T +S+A+T   TC  GF  D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGG- 275
           D  V+  +      +  M SN+LA+       D+   E R +     ++      E  G 
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251

Query: 276 -DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
            D +G+P WLS  DRRLL    STI+AD  VA DGSG F TVA A+ AAP  S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311

Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + R
Sbjct: 312 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371

Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
           D+TF+NTAGPSKHQAVALR+ +D +  Y C++  YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431

Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
           FGNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491

Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMNPGPGAAVGQ 575
           PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y   EY+N G GA    
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTY--REYLNRGGGAGTAN 551

Query: 576 RVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
           RVKW GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 RVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]
BLAST of CSPI05G03170 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 413.7 bits (1062), Expect = 2.6e-115
Identity = 223/462 (48.27%), Postives = 294/462 (63.64%), Query Frame = 0 

Query: 152 SAYEDCMELLNDAIDAFSLSLFSKDASN----------HDIMTWLSAALTYHDTCTAGFQ 211
           +A  DC++LL+ A +  S  + +  + N           D+ TW+SAAL+  DTC  GF+
Sbjct: 79  NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138

Query: 212 DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESSTTSWAAENG 271
               + +K  V   LS +   + N L +         P    K + +   + T   A +G
Sbjct: 139 GTNGI-IKKIVAGGLSKVGTTVRNLLTMVHS------PPSKPKPKPIKAQTMTK--AHSG 198

Query: 272 GDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 331
                FP+W+   DR+LL     T+ AD VVA DG+G F T+++A+ AAP  S +R +I+
Sbjct: 199 --FSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIH 258

Query: 332 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 391
           +K G Y  EN+++ +KK N+M VGDG   TVI+G +S  D  TTFR+ATFA SG   I R
Sbjct: 259 VKRGVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIAR 318

Query: 392 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 451
           D+TF+NTAGP KHQAVA+R   D  V Y C + GYQDTLY HS RQFFREC I GT+DFI
Sbjct: 319 DITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFI 378

Query: 452 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 511
           FG+A  VFQSC I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +  + 
Sbjct: 379 FGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT 438

Query: 512 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYEYMNPGPGAAVGQ 571
            T+LGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYG  EYMN GPGA++ +
Sbjct: 439 ATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYG--EYMNSGPGASLDR 498

Query: 572 RVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
           RVKWPGY V+ ++ EA+ FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 RVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
BLAST of CSPI05G03170 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FK051.3e-19961.24Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B01.8e-19357.57Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
O490062.3e-12943.99Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425345.2e-12945.19Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839483.4e-12844.52Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
BLAST of CSPI05G03170 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KJG10.0e+0099.50Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A1S3AUZ60.0e+0095.55Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A6J1E2V19.4e-29184.82Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1IA522.6e-28884.07Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1H2K19.8e-28083.44Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
BLAST of CSPI05G03170 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011654619.10.0e+0099.50probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
XP_008437596.10.0e+0095.55PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_038874841.16.0e-30889.44probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_022922302.11.9e-29084.82probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata][more]
KAG6579321.12.5e-29084.82putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
BLAST of CSPI05G03170 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G53370.18.9e-20161.24pectin methylesterase PCR fragment F [more]
AT3G49220.11.2e-19457.57Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.11.7e-13043.99pectin methylesterase 3 [more]
AT1G53830.13.7e-13045.19pectin methylesterase 2 [more]
AT3G43270.12.6e-11548.27Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 81..229
e-value: 4.3E-41
score: 152.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..231
e-value: 2.3E-33
score: 113.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 85..229
e-value: 4.4E-31
score: 108.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 283..603
e-value: 3.8E-119
score: 399.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 289..588
e-value: 7.3E-142
score: 471.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 83..233
e-value: 3.2E-36
score: 126.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 84..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 37..603
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 37..603
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 89..235
e-value: 1.0233E-36
score: 132.177
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 434..443
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 284..602

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G03170.1CSPI05G03170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity