CSPI05G03120 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G03120
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	AB hydrolase-1 domain-containing protein
Location	Chr5: 3481563 .. 3482381 (+)
RNA-Seq Expression	CSPI05G03120
Synteny	CSPI05G03120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTATTTGTTTACAACGACCAGGCACGATGTTATTGCTCAGATGTATTATGCTAGAAGACTTGCCGAAAAGCTGTATCCTGTTGCTAGAATGGTAGATCTCCATGGAGGTCATCTAGTCAGTCGAGAGAGAACCGAAGAGGTGTATTATTTTCACCACAATCGATCTAAATATCGTTTTTCACTATGTCTAGTTGACAACAGAAACTGATGAGATAAGTTTGTTTTCTATGAAAATTTAGGTAAATCAAGCTCTTCTTGACTTAATCAGAGCCTCAGAAACAAAGATGAGTCCACATGATTGGACAAACTTGCCAAAGAAAAGCTCATGTGAGTGTTTTTCTTTTAGTATTCCTCTGTTTCTCTTTGGTTCTTGGGAAATGCTGAAAAATACTTTCTTCCTAACAGGGTGGATGGAGGAGAGAATGGCTTTTGTAACAATGAAGACTGAAGGAGGAAGCAGTATCTCCTCCAAACCTTTCTTGTTAGAGAAACTCCATCTTTTCTTCTTGTATTTCTTTGGTCTGATTCTTTTGATTTTCGGGTATCTCCGGAACACCATCAAAAGTTTGAAACCCACCCGAGTTGGAGCCACCCTTACGTGAATAAACATTCCAAAGACCCTAAATTTTTACAGGTGAGTTATCTGAATATCCTCTACTTTCATTGAACTCAAACTAAAAGAGAGAAGTGTATAAGTTGGTTTTTGCTTCAACTTTGTTGCAGAGTCTTTCATTTTTGCTATGTGATGGGCAATGCTCCAACCGTCAAAGTGAAGTTGGGGAATGAGTTCTCTTCTAATATTGAGAACTTTTAA

mRNA sequence

ATGACTTATTTGTTTACAACGACCAGGCACGATGTTATTGCTCAGATGTATTATGCTAGAAGACTTGCCGAAAAGCTGTATCCTGTTGCTAGAATGGTAGATCTCCATGGAGGTCATCTAGTCAGTCGAGAGAGAACCGAAGAGGTAAATCAAGCTCTTCTTGACTTAATCAGAGCCTCAGAAACAAAGATGAGTCCACATGATTGGACAAACTTGCCAAAGAAAAGCTCATGGTGGATGGAGGAGAGAATGGCTTTTGTAACAATGAAGACTGAAGGAGGAAGCAGTATCTCCTCCAAACCTTTCTTGTTAGAGAAACTCCATCTTTTCTTCTTGTATTTCTTTGGTCTGATTCTTTTGATTTTCGGAGTCTTTCATTTTTGCTATGTGATGGGCAATGCTCCAACCGTCAAAGTGAAGTTGGGGAATGAGTTCTCTTCTAATATTGAGAACTTTTAA

Coding sequence (CDS)

ATGACTTATTTGTTTACAACGACCAGGCACGATGTTATTGCTCAGATGTATTATGCTAGAAGACTTGCCGAAAAGCTGTATCCTGTTGCTAGAATGGTAGATCTCCATGGAGGTCATCTAGTCAGTCGAGAGAGAACCGAAGAGGTAAATCAAGCTCTTCTTGACTTAATCAGAGCCTCAGAAACAAAGATGAGTCCACATGATTGGACAAACTTGCCAAAGAAAAGCTCATGGTGGATGGAGGAGAGAATGGCTTTTGTAACAATGAAGACTGAAGGAGGAAGCAGTATCTCCTCCAAACCTTTCTTGTTAGAGAAACTCCATCTTTTCTTCTTGTATTTCTTTGGTCTGATTCTTTTGATTTTCGGAGTCTTTCATTTTTGCTATGTGATGGGCAATGCTCCAACCGTCAAAGTGAAGTTGGGGAATGAGTTCTCTTCTAATATTGAGAACTTTTAA

Protein sequence

MTYLFTTTRHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHDWTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFGVFHFCYVMGNAPTVKVKLGNEFSSNIENF*

Homology

[Close]

BLAST of CSPI05G03120 vs. ExPASy TrEMBL
Match: A0A5A7TMC3 (Putative aminoacrylate hydrolase RutD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00420 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.5e-72
Identity = 137/144 (95.14%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFGVFHFC 128
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISS+PFLLEKLHLFFLYFFGLILL+FGVF FC
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSQPFLLEKLHLFFLYFFGLILLVFGVFQFC 388

Query: 129 YVMGNAPTVKVKLGNEFSSNIENF 153
           YVM N P VKVKLGNEFSSN+ENF
Sbjct: 389 YVMSNGPIVKVKLGNEFSSNVENF 412

[Close]

BLAST of CSPI05G03120 vs. ExPASy TrEMBL
Match: A0A0A0KLW7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139370 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 6.9e-57
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLI+ASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIKASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 124
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 383

[Close]

BLAST of CSPI05G03120 vs. ExPASy TrEMBL
Match: A0A1S3AUJ9 (putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.2e-56
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 124
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISS+PFLLEKLHLFFLYFFGLILL+FG
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSQPFLLEKLHLFFLYFFGLILLVFG 383

[Close]

BLAST of CSPI05G03120 vs. ExPASy TrEMBL
Match: A0A6J1DV10 (uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024626 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 3.4e-48
Identity = 97/114 (85.09%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQ++YAR+LAE+LYPVARMVDLHGGHLVSRERT+EVNQALLDLIRASETKM+PHD
Sbjct: 275 RHDVIAQIFYARQLAERLYPVARMVDLHGGHLVSRERTKEVNQALLDLIRASETKMNPHD 334

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNLPKKSSWWMEERM+ VT+KTE GSS+SSKPFLLE+LHL FLY  GLIL+ F
Sbjct: 335 WTNLPKKSSWWMEERMSLVTLKTEAGSSVSSKPFLLERLHLCFLYLLGLILMAF 388

[Close]

BLAST of CSPI05G03120 vs. ExPASy TrEMBL
Match: A0A6J1H2I9 (uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC111459843 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 3.4e-48
Identity = 99/115 (86.09%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           R DVIAQ+YYAR+LAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSP D
Sbjct: 275 RQDVIAQIYYARKLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPQD 334

Query: 69  WTNLPKKSSWWMEERMAFVTMKTE-GGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNLPKKSSWWMEERM+ +T+KTE GGS++S+KPFLLE+LHLFFLY FGLIL +F
Sbjct: 335 WTNLPKKSSWWMEERMSLITLKTEGGGSTVSTKPFLLERLHLFFLYLFGLILFMF 389

[Close]

BLAST of CSPI05G03120 vs. NCBI nr
Match: KAA0042545.1 (putative aminoacrylate hydrolase RutD [Cucumis melo var. makuwa] >TYK05949.1 putative aminoacrylate hydrolase RutD [Cucumis melo var. makuwa])

HSP 1 Score: 280.4 bits (716), Expect = 9.2e-72
Identity = 137/144 (95.14%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFGVFHFC 128
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISS+PFLLEKLHLFFLYFFGLILL+FGVF FC
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSQPFLLEKLHLFFLYFFGLILLVFGVFQFC 388

Query: 129 YVMGNAPTVKVKLGNEFSSNIENF 153
           YVM N P VKVKLGNEFSSN+ENF
Sbjct: 389 YVMSNGPIVKVKLGNEFSSNVENF 412

[Close]

BLAST of CSPI05G03120 vs. NCBI nr
Match: XP_004145933.1 (uncharacterized protein LOC101203096 [Cucumis sativus] >KGN49859.1 hypothetical protein Csa_000422 [Cucumis sativus])

HSP 1 Score: 229.9 bits (585), Expect = 1.4e-56
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLI+ASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIKASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 124
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 383

[Close]

BLAST of CSPI05G03120 vs. NCBI nr
Match: XP_008437608.1 (PREDICTED: putative aminoacrylate hydrolase RutD [Cucumis melo])

HSP 1 Score: 229.2 bits (583), Expect = 2.4e-56
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIFG 124
           WTNLPKKSSWWMEERMAFVTMKTEGGSSISS+PFLLEKLHLFFLYFFGLILL+FG
Sbjct: 329 WTNLPKKSSWWMEERMAFVTMKTEGGSSISSQPFLLEKLHLFFLYFFGLILLVFG 383

[Close]

BLAST of CSPI05G03120 vs. NCBI nr
Match: XP_038875890.1 (putative aminoacrylate hydrolase RutD [Benincasa hispida])

HSP 1 Score: 223.4 bits (568), Expect = 1.3e-54
Identity = 110/114 (96.49%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD
Sbjct: 269 RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 328

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNLPKKSSWWMEERMAFVT+KTEGGSSISSKPFLLE+LHLFFLY FGLILL+F
Sbjct: 329 WTNLPKKSSWWMEERMAFVTLKTEGGSSISSKPFLLERLHLFFLYIFGLILLVF 382

[Close]

BLAST of CSPI05G03120 vs. NCBI nr
Match: XP_022958692.1 (uncharacterized protein LOC111459843 [Cucurbita moschata])

HSP 1 Score: 201.1 bits (510), Expect = 7.1e-48
Identity = 99/115 (86.09%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           R DVIAQ+YYAR+LAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSP D
Sbjct: 275 RQDVIAQIYYARKLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPQD 334

Query: 69  WTNLPKKSSWWMEERMAFVTMKTE-GGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNLPKKSSWWMEERM+ +T+KTE GGS++S+KPFLLE+LHLFFLY FGLIL +F
Sbjct: 335 WTNLPKKSSWWMEERMSLITLKTEGGGSTVSTKPFLLERLHLFFLYLFGLILFMF 389

[Close]

BLAST of CSPI05G03120 vs. TAIR 10
Match: AT5G53050.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 4.9e-31
Identity = 67/114 (58.77%), Postives = 84/114 (73.68%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQ+ YARRLA++LYPVARMVDLHGGHLVS ERTEEVN+ALL+LI+ASE K    D
Sbjct: 263 RHDVIAQICYARRLAQRLYPVARMVDLHGGHLVSHERTEEVNKALLELIKASEMKKISTD 322

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNL  ++  + + R+  +   TEG +  S   F+ EK H F L+ FGL++L F
Sbjct: 323 WTNLTMETPGYFKRRLTLIRSSTEGKNVASPAHFIAEKFHGFLLFLFGLLVLAF 376

[Close]

BLAST of CSPI05G03120 vs. TAIR 10
Match: AT5G53050.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 4.9e-31
Identity = 67/114 (58.77%), Postives = 84/114 (73.68%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEVNQALLDLIRASETKMSPHD 68
           RHDVIAQ+ YARRLA++LYPVARMVDLHGGHLVS ERTEEVN+ALL+LI+ASE K    D
Sbjct: 263 RHDVIAQICYARRLAQRLYPVARMVDLHGGHLVSHERTEEVNKALLELIKASEMKKISTD 322

Query: 69  WTNLPKKSSWWMEERMAFVTMKTEGGSSISSKPFLLEKLHLFFLYFFGLILLIF 123
           WTNL  ++  + + R+  +   TEG +  S   F+ EK H F L+ FGL++L F
Sbjct: 323 WTNLTMETPGYFKRRLTLIRSSTEGKNVASPAHFIAEKFHGFLLFLFGLLVLAF 376

[Close]

BLAST of CSPI05G03120 vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-14
Identity = 36/41 (87.80%), Postives = 39/41 (95.12%), Query Frame = 0


Query: 9   RHDVIAQMYYARRLAEKLYPVARMVDLHGGHLVSRERTEEV 50
           RHDVIAQ+ YARRLA++LYPVARMVDLHGGHLVS ERTEEV
Sbjct: 263 RHDVIAQICYARRLAQRLYPVARMVDLHGGHLVSHERTEEV 303

The following BLAST results are available for this feature:

BLAST of CSPI05G03120 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI05G03120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TMC3	4.5e-72	95.14	Putative aminoacrylate hydrolase RutD OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0KLW7	6.9e-57	99.13	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139...	[more]
A0A1S3AUJ9	1.2e-56	98.26	putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE...	[more]
A0A6J1DV10	3.4e-48	85.09	uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1H2I9	3.4e-48	86.09	uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC1114598...	[more]

BLAST of CSPI05G03120 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0042545.1	9.2e-72	95.14	putative aminoacrylate hydrolase RutD [Cucumis melo var. makuwa] >TYK05949.1 put...	[more]
XP_004145933.1	1.4e-56	99.13	uncharacterized protein LOC101203096 [Cucumis sativus] >KGN49859.1 hypothetical ...	[more]
XP_008437608.1	2.4e-56	98.26	PREDICTED: putative aminoacrylate hydrolase RutD [Cucumis melo]	[more]
XP_038875890.1	1.3e-54	96.49	putative aminoacrylate hydrolase RutD [Benincasa hispida]	[more]
XP_022958692.1	7.1e-48	86.09	uncharacterized protein LOC111459843 [Cucurbita moschata]	[more]

BLAST of CSPI05G03120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53050.2	4.9e-31	58.77	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.3	4.9e-31	58.77	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.1	1.9e-14	87.80	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 2..60 e-value: 1.4E-5 score: 26.9
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 8..86
None	No IPR available	PANTHER	PTHR43329:SF109	BNAA10G06420D PROTEIN	coord: 8..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G03120.1	CSPI05G03120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016787	hydrolase activity