CSPI05G02640 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G02640
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionIleal sodium/bile acid cotransporter, putative
LocationChr5: 3237459 .. 3238173 (-)
RNA-Seq ExpressionCSPI05G02640
SyntenyCSPI05G02640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAACCCCCTTGAGAGTGTATTGAAAAAACTATCATTTTCTTTTCTTTTTTTAGCTTTCTTTTCCAATGGCTTCAACTTCACACCAAACCTCTTTAGATATGAGTGCATTTTCTTCTTTTATCCGAACAATCAGCCAAAGAAACTATGGACTTGGCTGTTGCTGCACACCATCAAACTGCTTACCAGTTTTCATTAGAATGCAACAAAGCCCTCAAGCAACAGCCGATAGCTCTCAAAAACTTGGCAAGATAATTCTTAGCCTTAGTTTTCAAGCAGTTTTAGCCTTGTTCATTAGCTCACCACCAACTTCCCCTCCTCCACTTTTGATACACTTTTTTGCTGCTGCTGTTTTCATTAGCTTTGCTGTTTCATTTGCTGCTCTTTTCCTTCATAACTCCTTCCCGAGAACCGCCCATTTATTCGAGAAGATTGGTGCGCTTTTTTCTGCATTTGGTGTGTGTTTCATAGCAAGCTTTCTTCTAGTTCATCAGAACTTTGCTTGGATTTGTTGGGTGGCATGTACCTTCTCCATCATTGTCTTTGCTTTATCATTTAAGTGACATTTTTTAGACATAAAATGGCCATTTCTTTACCCATCCATTAGGCATTTTGTTTGACCTTCTTCCATGGCTCCATCAAGAATAATGAGCTCTCTCTGTATGGATTTTTTTTCCCAGTTGAGATAATATTATTGAAGAAAAAGTTGTTCCCAC

mRNA sequence

GGGAACCCCCTTGAGAGTGTATTGAAAAAACTATCATTTTCTTTTCTTTTTTTAGCTTTCTTTTCCAATGGCTTCAACTTCACACCAAACCTCTTTAGATATGAGTGCATTTTCTTCTTTTATCCGAACAATCAGCCAAAGAAACTATGGACTTGGCTGTTGCTGCACACCATCAAACTGCTTACCAGTTTTCATTAGAATGCAACAAAGCCCTCAAGCAACAGCCGATAGCTCTCAAAAACTTGGCAAGATAATTCTTAGCCTTAGTTTTCAAGCAGTTTTAGCCTTGTTCATTAGCTCACCACCAACTTCCCCTCCTCCACTTTTGATACACTTTTTTGCTGCTGCTGTTTTCATTAGCTTTGCTGTTTCATTTGCTGCTCTTTTCCTTCATAACTCCTTCCCGAGAACCGCCCATTTATTCGAGAAGATTGGTGCGCTTTTTTCTGCATTTGGTGTGTGTTTCATAGCAAGCTTTCTTCTAGTTCATCAGAACTTTGCTTGGATTTGTTGGGTGGCATGTACCTTCTCCATCATTGTCTTTGCTTTATCATTTAAGTGACATTTTTTAGACATAAAATGGCCATTTCTTTACCCATCCATTAGGCATTTTGTTTGACCTTCTTCCATGGCTCCATCAAGAATAATGAGCTCTCTCTGTATGGATTTTTTTTCCCAGTTGAGATAATATTATTGAAGAAAAAGTTGTTCCCAC

Coding sequence (CDS)

ATGGCTTCAACTTCACACCAAACCTCTTTAGATATGAGTGCATTTTCTTCTTTTATCCGAACAATCAGCCAAAGAAACTATGGACTTGGCTGTTGCTGCACACCATCAAACTGCTTACCAGTTTTCATTAGAATGCAACAAAGCCCTCAAGCAACAGCCGATAGCTCTCAAAAACTTGGCAAGATAATTCTTAGCCTTAGTTTTCAAGCAGTTTTAGCCTTGTTCATTAGCTCACCACCAACTTCCCCTCCTCCACTTTTGATACACTTTTTTGCTGCTGCTGTTTTCATTAGCTTTGCTGTTTCATTTGCTGCTCTTTTCCTTCATAACTCCTTCCCGAGAACCGCCCATTTATTCGAGAAGATTGGTGCGCTTTTTTCTGCATTTGGTGTGTGTTTCATAGCAAGCTTTCTTCTAGTTCATCAGAACTTTGCTTGGATTTGTTGGGTGGCATGTACCTTCTCCATCATTGTCTTTGCTTTATCATTTAAGTGA

Protein sequence

MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLGKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFEKIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK*
Homology
BLAST of CSPI05G02640 vs. ExPASy TrEMBL
Match: A0A0A0KJP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G137410 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 9.3e-84
Identity = 163/164 (99.39%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60
           MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG
Sbjct: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60

Query: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120
           KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE
Sbjct: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120

Query: 121 KIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           K+GALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK
Sbjct: 121 KVGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 164

BLAST of CSPI05G02640 vs. ExPASy TrEMBL
Match: A0A5A7U7U1 (Putative Ileal sodium/bile acid cotransporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00420 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.2e-32
Identity = 88/154 (57.14%), Postives = 108/154 (70.13%), Query Frame = 0

Query: 12  MSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQ-SPQATADSSQKLGKIILSLSFQA 71
           M+   SFI  I++RN  +       N LP+ I MQ+ SP  ++ +   +GK IL L+FQA
Sbjct: 1   MNELYSFITAINERNPEI-------NSLPICITMQRPSPANSSKTENNVGKTILGLTFQA 60

Query: 72  VLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFEKIGALFSAFG 131
           VLALFI+S P S PPLL H FAAAV ISFAVSFA +FL N FPR A LFEKIGAL +A G
Sbjct: 61  VLALFITS-PNSSPPLLTHLFAAAVLISFAVSFAGIFLQNGFPRIALLFEKIGALIAAIG 120

Query: 132 VCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           VC +AS LL+HQNFAWI W+A  FS++ F LSF+
Sbjct: 121 VCIVAS-LLIHQNFAWISWLASGFSLMAFVLSFR 145

BLAST of CSPI05G02640 vs. ExPASy TrEMBL
Match: A0A0A0KJN8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136870 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.9e-32
Identity = 90/165 (54.55%), Postives = 113/165 (68.48%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQ-QSPQATADSSQKL 60
           M S+S Q S+DM+   S I +I++RN  +       N LP+ I MQ  SP  ++ +   +
Sbjct: 1   MESSSQQISIDMNHLYSLITSINERNPEI-------NGLPICIIMQTASPANSSKTENNV 60

Query: 61  GKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLF 120
           G  IL L+FQAVLALFI+S  TS PPLL H F AAV ISFAVSF  +FL + FPR A LF
Sbjct: 61  GTTILGLTFQAVLALFITS-STSSPPLLTHLFGAAVLISFAVSFPGVFLQDGFPRIALLF 120

Query: 121 EKIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           EKIGAL +A GVC +AS LL+HQNFAWI W+AC FS++ F LSF+
Sbjct: 121 EKIGALIAAIGVCILAS-LLIHQNFAWISWLACGFSLMAFLLSFR 156

BLAST of CSPI05G02640 vs. ExPASy TrEMBL
Match: A0A6J1CY58 (uncharacterized protein LOC111015658 OS=Momordica charantia OX=3673 GN=LOC111015658 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.3e-24
Identity = 77/127 (60.63%), Postives = 89/127 (70.08%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGL------GCCCTPSNCLPVFIRMQQSPQATAD 60
           MAST  QTS+DM+A SSFI  I++RN G+      G   TPS+CLP+ IRMQ+ P   A 
Sbjct: 1   MASTPQQTSVDMNALSSFITAINERNLGISSFTWGGGTSTPSDCLPICIRMQRPP--PAK 60

Query: 61  SSQKLGKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPR 120
           SSQ LGK IL L+FQAVLALFIS  P+SPP L    F AAV ISFAVSFA LFL  ++PR
Sbjct: 61  SSQSLGKTILGLTFQAVLALFISL-PSSPPQLPTLLFGAAVLISFAVSFAGLFLQTAYPR 120

Query: 121 TAHLFEK 122
            A LFEK
Sbjct: 121 MALLFEK 124

BLAST of CSPI05G02640 vs. ExPASy TrEMBL
Match: A0A0A0KQ03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136900 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 3.2e-23
Identity = 70/168 (41.67%), Postives = 99/168 (58.93%), Query Frame = 0

Query: 1   MAST-SHQTSLDMSAFSSFIRTISQRNYGLGCCC---TPSNCLPVFIRMQQSPQATADSS 60
           MA T  H+ SLDM+  +S I  ++ RN G+  C      S+CLP++I+MQ+       +S
Sbjct: 1   MAETPPHEISLDMNKLNSLIFAVTNRNPGISSCTWGGAASDCLPIYIKMQRPSPV---NS 60

Query: 61  QKLGKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTA 120
            + G   LSL+FQA++ LF+S  P+S  PL    FAA +  SF  S+  + L   FP+TA
Sbjct: 61  PQFGNTFLSLTFQAIVGLFLSLNPSSSSPLPSRLFAAVMLTSFIFSYDGVILQKPFPKTA 120

Query: 121 HLFEKIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
            L +  GALF+A G C I S LL++ NF WICW+A    +  F +SFK
Sbjct: 121 QLLQTFGALFAAIGTCIIGS-LLLYPNFTWICWLAAGLILPAFIISFK 164

BLAST of CSPI05G02640 vs. NCBI nr
Match: KGN49808.1 (hypothetical protein Csa_004650 [Cucumis sativus])

HSP 1 Score: 319.3 bits (817), Expect = 1.9e-83
Identity = 163/164 (99.39%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60
           MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG
Sbjct: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60

Query: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120
           KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE
Sbjct: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120

Query: 121 KIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           K+GALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK
Sbjct: 121 KVGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 164

BLAST of CSPI05G02640 vs. NCBI nr
Match: KAA0050246.1 (putative Ileal sodium/bile acid cotransporter [Cucumis melo var. makuwa] >TYJ98160.1 putative Ileal sodium/bile acid cotransporter [Cucumis melo var. makuwa])

HSP 1 Score: 148.7 bits (374), Expect = 4.5e-32
Identity = 88/154 (57.14%), Postives = 108/154 (70.13%), Query Frame = 0

Query: 12  MSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQ-SPQATADSSQKLGKIILSLSFQA 71
           M+   SFI  I++RN  +       N LP+ I MQ+ SP  ++ +   +GK IL L+FQA
Sbjct: 1   MNELYSFITAINERNPEI-------NSLPICITMQRPSPANSSKTENNVGKTILGLTFQA 60

Query: 72  VLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFEKIGALFSAFG 131
           VLALFI+S P S PPLL H FAAAV ISFAVSFA +FL N FPR A LFEKIGAL +A G
Sbjct: 61  VLALFITS-PNSSPPLLTHLFAAAVLISFAVSFAGIFLQNGFPRIALLFEKIGALIAAIG 120

Query: 132 VCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           VC +AS LL+HQNFAWI W+A  FS++ F LSF+
Sbjct: 121 VCIVAS-LLIHQNFAWISWLASGFSLMAFVLSFR 145

BLAST of CSPI05G02640 vs. NCBI nr
Match: KGN49803.1 (hypothetical protein Csa_004681 [Cucumis sativus])

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-31
Identity = 90/165 (54.55%), Postives = 113/165 (68.48%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQ-QSPQATADSSQKL 60
           M S+S Q S+DM+   S I +I++RN  +       N LP+ I MQ  SP  ++ +   +
Sbjct: 1   MESSSQQISIDMNHLYSLITSINERNPEI-------NGLPICIIMQTASPANSSKTENNV 60

Query: 61  GKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLF 120
           G  IL L+FQAVLALFI+S  TS PPLL H F AAV ISFAVSF  +FL + FPR A LF
Sbjct: 61  GTTILGLTFQAVLALFITS-STSSPPLLTHLFGAAVLISFAVSFPGVFLQDGFPRIALLF 120

Query: 121 EKIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
           EKIGAL +A GVC +AS LL+HQNFAWI W+AC FS++ F LSF+
Sbjct: 121 EKIGALIAAIGVCILAS-LLIHQNFAWISWLACGFSLMAFLLSFR 156

BLAST of CSPI05G02640 vs. NCBI nr
Match: KAG6579132.1 (hypothetical protein SDJN03_23580, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.0 bits (323), Expect = 3.7e-26
Identity = 71/130 (54.62%), Postives = 95/130 (73.08%), Query Frame = 0

Query: 34  TPSNCLPVFIRMQQSPQATADSSQKLGKIILSLSFQAVLALFISSPPTSPPPLLIHFFAA 93
           TP +CLP++   +Q  +     S  +GK I+ L+ QA+LA+FISS P+S PPLL   F A
Sbjct: 5   TPRDCLPIWRAREQRLEPDKGHS-NVGKAIIGLTSQALLAMFISS-PSSSPPLLRVLFGA 64

Query: 94  AVFISFAVSFAALFLHNSFPRTAHLFEKIGALFSAFGVCFIASFLLVHQNFAWICWVACT 153
            +FISF +SFA +FL N+FP+ A LFEK+GALF+A GV  IASFLL+H+N+AWI  +AC 
Sbjct: 65  TMFISFLLSFAGIFLRNAFPKAARLFEKLGALFAAIGVSIIASFLLMHENYAWISGLACV 124

Query: 154 FSIIVFALSF 164
           FS+IVF LS+
Sbjct: 125 FSLIVFGLSY 132

BLAST of CSPI05G02640 vs. NCBI nr
Match: KAG6579137.1 (hypothetical protein SDJN03_23585, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 125.9 bits (315), Expect = 3.1e-25
Identity = 84/167 (50.30%), Postives = 103/167 (61.68%), Query Frame = 0

Query: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQ--- 60
           M ST    S+DM+ F      I   + GL     P+  +P        P    DSSQ   
Sbjct: 1   MGSTPQHVSIDMNQFC----PIQGASDGL----PPTTAMP-------RPPPPPDSSQTRN 60

Query: 61  KLGKIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAH 120
            LGKI+  L+FQAVLALFI SPPTS PPLL+H FAAA+ ISFA+S AALFL  ++PR A 
Sbjct: 61  NLGKIVFGLTFQAVLALFI-SPPTSCPPLLMHIFAAAMLISFALSLAALFLQIAYPRIAL 120

Query: 121 LFEKIGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 165
              KIGAL +A G C I S LL HQ+F+WI W+AC F+++ F LSFK
Sbjct: 121 SSGKIGALLAAIGACTITSVLLKHQHFSWIPWLACGFALMAFILSFK 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KJP29.3e-8499.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G137410 PE=4 SV=1[more]
A0A5A7U7U12.2e-3257.14Putative Ileal sodium/bile acid cotransporter OS=Cucumis melo var. makuwa OX=119... [more]
A0A0A0KJN84.9e-3254.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136870 PE=4 SV=1[more]
A0A6J1CY581.3e-2460.63uncharacterized protein LOC111015658 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A0A0KQ033.2e-2341.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136900 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KGN49808.11.9e-8399.39hypothetical protein Csa_004650 [Cucumis sativus][more]
KAA0050246.14.5e-3257.14putative Ileal sodium/bile acid cotransporter [Cucumis melo var. makuwa] >TYJ981... [more]
KGN49803.11.0e-3154.55hypothetical protein Csa_004681 [Cucumis sativus][more]
KAG6579132.13.7e-2654.62hypothetical protein SDJN03_23580, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6579137.13.1e-2550.30hypothetical protein SDJN03_23585, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02640.1CSPI05G02640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane