CSPI05G02360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G02360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr5: 3046946 .. 3049285 (+)
RNA-Seq ExpressionCSPI05G02360
SyntenyCSPI05G02360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATTCAATAAGCATTACACAAAACCCACTTTTTTTCCCCATCTTTGTGGTGGGAAAAGCTAATTCTTTCATTTGGGTTTTGATCATTTGAAAAATTAAGAAACCTTTTCATATTACCATAAAGGTGGAAACCAAAAGAAAAAAAAATTAAAAAAAAATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTGCTAATTATCAAGAGGAATGCCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTGTTGCCACTATTGATGAGGTTAAGAAGGGGTATAATTTGACTGATGGGTTTCTGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTACATACCAATGCCGACCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTAATGGTGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGTAATTCTGCTAAATCACTTGACTAATTAATCTCCACTACCTTACTTCCATTAAATATATTACAATTTTGTTGGCAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAACGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCGTATCCAAAGACCTTAAGGGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACTCAAGACACTGCCACTTTCAGTAAGTGAATGAATTTACCACATGGCAAATATGATCGGACAATTTGATGAAATCACTCACTATTTTTCTTTCAACTTACACGTATATTTATACTTGTAGTTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATCTCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATAGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCGAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACAATGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACGAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAAAACTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAAGCTAAAAGAAAAAATTGTGTTTATTTGATGACGAGTGTAATATAATTTATTTGTTTTCATTCCCCATTCGATATCAATTTATTGCCTTAGAATTGGAAGGCCATAAATGGTTACCACTTTCAGAAAAGGTGTGCATTATGTTTTAATGAGGAGGTTTAGCAACATATATAAAACTCTTTTTTTACCATTTTTTAATTGTTACAAAAAGTAAGAGAATTGTTCTTCATTTTGTGATTAATGTTGTCAAGCAAACATACATGGTGTTGTAATTTTATAAAATGACAATTGATTGCAATGTGCTTATTACGTTC

mRNA sequence

CTATTCAATAAGCATTACACAAAACCCACTTTTTTTCCCCATCTTTGTGGTGGGAAAAGCTAATTCTTTCATTTGGGTTTTGATCATTTGAAAAATTAAGAAACCTTTTCATATTACCATAAAGGTGGAAACCAAAAGAAAAAAAAATTAAAAAAAAATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTGCTAATTATCAAGAGGAATGCCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTGTTGCCACTATTGATGAGGTTAAGAAGGGGTATAATTTGACTGATGGGTTTCTGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTACATACCAATGCCGACCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTAATGGTGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAACGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCGTATCCAAAGACCTTAAGGGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACTCAAGACACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATCTCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATAGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCGAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACAATGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACGAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAAAACTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAAGCTAAAAGAAAAAATTGTGTTTATTTGATGACGAGTGTAATATAATTTATTTGTTTTCATTCCCCATTCGATATCAATTTATTGCCTTAGAATTGGAAGGCCATAAATGGTTACCACTTTCAGAAAAGGTGTGCATTATGTTTTAATGAGGAGGTTTAGCAACATATATAAAACTCTTTTTTTACCATTTTTTAATTGTTACAAAAAGTAAGAGAATTGTTCTTCATTTTGTGATTAATGTTGTCAAGCAAACATACATGGTGTTGTAATTTTATAAAATGACAATTGATTGCAATGTGCTTATTACGTTC

Coding sequence (CDS)

ATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTGCTAATTATCAAGAGGAATGCCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTGTTGCCACTATTGATGAGGTTAAGAAGGGGTATAATTTGACTGATGGGTTTCTGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTACATACCAATGCCGACCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTAATGGTGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAACGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCGTATCCAAAGACCTTAAGGGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACTCAAGACACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATCTCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATAGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCGAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACAATGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACGAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAAAACTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAAGCTAA

Protein sequence

MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQNTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS*
Homology
BLAST of CSPI05G02360 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.4e-128
Identity = 267/605 (44.13%), Postives = 363/605 (60.00%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA  +LSP+MKAV  IC + + +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA--NLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIE-----AANNRSIKMGVED 120
           TL        S+DP + +KA ++AT DE+ K  N T    +      + NN+++   ++ 
Sbjct: 61  TL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LDY 120

Query: 121 CRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLR 180
           C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  LR
Sbjct: 121 CKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK-----------------------A 240
           + + +G+  +  LT+NA+ I   V   +A    ++                        A
Sbjct: 181 KAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVA 240

Query: 241 QPSG--RRLLGTTEVDNDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDGS 300
            P G  RRLL   ++D  G PTW++GADRKL+A         RGGGA+V+ N VVAKDGS
Sbjct: 241 DPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGS 300

Query: 301 GQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKS 360
           GQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   NIFM+GDG +KT+++ N+S
Sbjct: 301 GQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRS 360

Query: 361 --NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGY 420
                G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGY
Sbjct: 361 VALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGY 420

Query: 421 QDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPK 480
           QDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR+    Q NTVTA+G     
Sbjct: 421 QDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGL 480

Query: 481 EVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWS 540
            +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + MGD I+P G+  W 
Sbjct: 481 GMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWD 540

Query: 541 GNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGP 565
           G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L    W+     P
Sbjct: 541 GESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVP 588

BLAST of CSPI05G02360 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.5e-125
Identity = 227/564 (40.25%), Postives = 351/564 (62.23%), Query Frame = 0

Query: 4   KVVLPGISLLLVVGVALAVVAIVNSNKSSDA-TEDLSPKMKAVSTICSTANYQEECQNTL 63
           + V+  IS +L++ + +AV   V+ NKS +A  E+++  +KA+  +C+  +Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E VK A  AT+ ++      +   +IE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 NGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL      +  +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIYKEYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGSFLQGKTWLPTTGGPYLLGLKS 567
              ++QG  W+P  G PY+LGL S
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of CSPI05G02360 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-125
Identity = 223/558 (39.96%), Postives = 344/558 (61.65%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNK---SSDATEDLSPKMKAVSTICSTANYQEECQNTLSNAA 69
           IS +L++ + +AV   V+ NK    S    +++  +KAV  +C+  +Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T+ ++      +   ++E   +   +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQ-TIMELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAG 189
           L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRG 249
           +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RKLL +  
Sbjct: 200 ELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRKLLQAAA 259

Query: 250 GGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIF 309
             + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+YKEYV + K M ++ 
Sbjct: 260 AYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLV 319

Query: 310 MYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQ 369
             GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RVQ
Sbjct: 320 FIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQ 379

Query: 370 SDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDN 429
           SD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  V QN  ++VR+P+ N
Sbjct: 380 SDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPN 439

Query: 430 QQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTM 489
           Q   +TA+GR DP+E +G V   C I  E    A +     +LGRPWKEY+RT+IM + +
Sbjct: 440 QACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFI 499

Query: 490 GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSF 549
            DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++FT   +
Sbjct: 500 PDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQY 559

Query: 550 LQGKTWLPTTGGPYLLGL 565
           +QG  W+P  G PY  GL
Sbjct: 560 IQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02360 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-125
Identity = 255/598 (42.64%), Postives = 364/598 (60.87%), Query Frame = 0

Query: 4   KVVLPGISLLLV----VGVALAVVAIVN---------SNKSSDA---TEDLSPKM----- 63
           +++L  +S+L+V    +G A A V   N         +NKS D+   +E  SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSTANYQEECQNTLSN-AAHNASSDDPKEYVKAAIVATIDEVKKG 123
                    K + T+C++  Y+  CQNTL N    +    DP+  +K+AIVA  D++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNW 183
           +      L     N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRV---LSLKTENKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASF-----G 243
           L++V+SYQ++C+DG EE   +L+ +++   N +  LTSN+LA++ ++   L+S       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKT 303
           L L+A+ S +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAK--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 ISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGF 363
           I+AAL A P   +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALET 543
           HNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 565
             Y EY N GP   T  RV+W G +V+ + EAM+FT G FLQG+ W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of CSPI05G02360 vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-125
Identity = 266/613 (43.39%), Postives = 367/613 (59.87%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA  +LSP+MKAV  IC   + +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYI--NKNGDA--NLSPQMKAVRGICEATSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNL---TDGFLIE--AANNRSIKMGVED 120
           TL        SDDP + +KA ++AT D + +  N    T+G L    + NN+++   ++ 
Sbjct: 61  TL----EPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAV---LDY 120

Query: 121 CRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLR 180
           C+ +  +A++ L      +GE DL+    ++  +K WLT V +YQ  CLD +EE D  LR
Sbjct: 121 CKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK------------------------ 240
           + + +G+  +  LTSNA+ I   V   +A   L+++                        
Sbjct: 181 KTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGT 240

Query: 241 ---------AQPSG--RRLLGTTEVDNDGYPTWLTGADRKLLASRG-----GGAKVKPNA 300
                    A P G  RRLL   ++D  G PTW++GADRKL+   G     GGA+++   
Sbjct: 241 PPVADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATF 300

Query: 301 VVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKT 360
           VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY+E V I K   NIFM+GDG +KT
Sbjct: 301 VVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKT 360

Query: 361 IVTGNKSNR--GGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFF 420
           +++ N+S +   G TT  + T     EGF+ K +GF+NTAGP GHQAVA+RV  DR+  F
Sbjct: 361 VISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIF 420

Query: 421 NCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTA 480
           NCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S TVIQNSLI+VR+    Q NTVTA
Sbjct: 421 NCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTA 480

Query: 481 NGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQP 540
           +G      +  G+V+ NCRIVP++KL AER  + ++LGRPWK+++ TVI+ S +GD I+P
Sbjct: 481 DGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRP 540

Query: 541 VGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVI-GRNEAMQFTAGSFLQGKT 565
            G+  W G    ++C Y+EY NRGPGA+TNRRV W  VK+     E   FT  ++L    
Sbjct: 541 EGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPIN 595

BLAST of CSPI05G02360 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 542/567 (95.59%), Postives = 558/567 (98.41%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSD-ATEDLSPKMKAVSTICSTANYQEECQ 60
           MVNKV+LPGISL+LVVGVALAVVAIV+SNKSSD ATEDLSPKMKAVSTIC+ ANYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAI++TI+EVKKGYNLTDGF+IEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240
           DGL+ AGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPK LRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 567
           QFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02360 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 541/567 (95.41%), Postives = 558/567 (98.41%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSD-ATEDLSPKMKAVSTICSTANYQEECQ 60
           MVNKV+LPGI+L+LVVGVALAVVAIV+SNKSSD ATEDLSPKMKAVSTIC+ ANYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAI++TI+EVKKGYNLTDGF+IEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240
           DGL+ AGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPK LRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 567
           QFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02360 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.6e-276
Identity = 470/566 (83.04%), Postives = 511/566 (90.28%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           MV KVV+  ++ LLVVGV LAV A+VN N SSD T D+SPKMKAVS+ICS  +YQ+ECQ 
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLL 120
           TL N  HN+S DDPKE++KAAI ATI+E+KKGYNLTD  ++EAANN SIKM V+DCRDLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQD 180
           Q AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GL+ A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVDNDGYPTWLTGADRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITK 300
           LLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPK L+GRYVIYVKAGIY EY+ +TK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 360
           DMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540
           IME+T+GDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAMQ
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGKTWLPTTGGPYLLGLKS 567
           FTAG FL G+ WLP TGGPYLLGLK+
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CSPI05G02360 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 8.0e-276
Identity = 467/566 (82.51%), Postives = 513/566 (90.64%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           MV KVV+ GISL+LVVGVALAVVAIVN + SSD T +LSPKMKAV++ICS A+YQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLL 120
           TL +  +N+S +DPKE++KAAI +TI+E+KKGYNLTD  ++EAANN +IKM V+DC+DLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQD 180
           Q AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GL+ AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITK 300
           LLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPK L+GRYVIYVKAG+Y E + +TK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 360
           DMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS T+IQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540
           IMEST+GDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGKTWLPTTGGPYLLGLKS 567
           FTAG FL GK WLP TGGPYLLGLK+
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CSPI05G02360 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.8e-275
Identity = 464/557 (83.30%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQNTLSNAAHNA 69
           ++ LLVVGV LAV A+VN N SSD T D+SPKMKAVS+ICS  +YQ+ECQ TL N  HN+
Sbjct: 3   VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNS 62

Query: 70  SSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQLQA 129
           S DDPKE++KAAI ATI+E+KKGYNLTD  ++EAANN SIKM V+DCRDLLQ AID+L A
Sbjct: 63  SVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHA 122

Query: 130 SYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLT 189
           SYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ GL+ A KLT
Sbjct: 123 SYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLT 182

Query: 190 SNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRGGGA 249
           SNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVDNDG+PTWLTGADRKLLA++ GG 
Sbjct: 183 SNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRGGV 242

Query: 250 KVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYG 309
           +VKPNAVVAKDGSGQ+KTI+AALAAYPK L+GRYVIYVKAGIY EY+ +TKDMKN+FMYG
Sbjct: 243 RVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYG 302

Query: 310 DGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDR 369
           DGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQAVALRVQSDR
Sbjct: 303 DGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR 362

Query: 370 SAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQN 429
           SAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLIIVRRPMDNQQN
Sbjct: 363 SAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN 422

Query: 430 TVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDF 489
           TVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME+T+GDF
Sbjct: 423 TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDF 482

Query: 490 IQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQG 549
           IQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAMQFTAG FL G
Sbjct: 483 IQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLG 542

Query: 550 KTWLPTTGGPYLLGLKS 567
           + WLP TGGPYLLGLK+
Sbjct: 543 RAWLPATGGPYLLGLKN 559

BLAST of CSPI05G02360 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 542/567 (95.59%), Postives = 558/567 (98.41%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSD-ATEDLSPKMKAVSTICSTANYQEECQ 60
           MVNKV+LPGISL+LVVGVALAVVAIV+SNKSSD ATEDLSPKMKAVSTIC+ ANYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAI++TI+EVKKGYNLTDGF+IEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240
           DGL+ AGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPK LRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 567
           QFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02360 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 541/567 (95.41%), Postives = 558/567 (98.41%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSD-ATEDLSPKMKAVSTICSTANYQEECQ 60
           MVNKV+LPGI+L+LVVGVALAVVAIV+SNKSSD ATEDLSPKMKAVSTIC+ ANYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAI++TI+EVKKGYNLTDGF+IEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240
           DGL+ AGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPK LRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 567
           QFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02360 vs. NCBI nr
Match: XP_038875878.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.9e-288
Identity = 494/565 (87.43%), Postives = 531/565 (93.98%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           MVNKVVLPGISL+LVVGVALAVVA+VN N +S  TE+LSPKMKAVSTICS  NYQEECQN
Sbjct: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNS-TTENLSPKMKAVSTICSNTNYQEECQN 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLL 120
           TL+N AHNASSDDPKEYVKAAI+AT++EVKKGYNLTDGF+IEAANN SIKMGVEDC+DLL
Sbjct: 61  TLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLL 120

Query: 121 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQD 180
           QFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQD
Sbjct: 121 QFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQD 180

Query: 181 GLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GL+ A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EV +DG+PTW++GADRK
Sbjct: 181 GLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRK 240

Query: 241 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITK 300
           LLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPK L+GRYVIYVKAGIY EYV +TK
Sbjct: 241 LLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 360
           DM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQA
Sbjct: 301 DMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIV 420
           VALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTT+IQNSLIIV
Sbjct: 361 VALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540
           IME+T+GDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA++
Sbjct: 481 IMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALK 540

Query: 541 FTAGSFLQGKTWLPTTGGPYLLGLK 566
           FTAGSFLQG +WLP TGGPYLLGLK
Sbjct: 541 FTAGSFLQGGSWLPGTGGPYLLGLK 563

BLAST of CSPI05G02360 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 961.4 bits (2484), Expect = 3.3e-276
Identity = 470/566 (83.04%), Postives = 511/566 (90.28%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           MV KVV+  ++ LLVVGV LAV A+VN N SSD T D+SPKMKAVS+ICS  +YQ+ECQ 
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLL 120
           TL N  HN+S DDPKE++KAAI ATI+E+KKGYNLTD  ++EAANN SIKM V+DCRDLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQD 180
           Q AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GL+ A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVDNDGYPTWLTGADRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITK 300
           LLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPK L+GRYVIYVKAGIY EY+ +TK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 360
           DMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540
           IME+T+GDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAMQ
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGKTWLPTTGGPYLLGLKS 567
           FTAG FL G+ WLP TGGPYLLGLK+
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CSPI05G02360 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 960.7 bits (2482), Expect = 5.7e-276
Identity = 470/566 (83.04%), Postives = 518/566 (91.52%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDL-SPKMKAVSTICSTANYQEECQ 60
           MV KVV+ GISL+LVVGVALAVVA+V  N +SD   DL SPKMKAVSTICST +YQ+ECQ
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDV--DLSSPKMKAVSTICSTTDYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120
           NTL+N A NAS DDPKEY KAA++ATI E+ KGYNLT   ++EAANN SIKM V+DC+DL
Sbjct: 61  NTLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240
           DGL+ AGKLTSNALAIV AVSDIL +FGLQLK QPSGRRLL TTE+ +DG+PTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGA+VKPNAVVAKDGSGQ+K+I AALAAYPK LRGRYVIYVKAGIY EY+ +T
Sbjct: 241 KLLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG K+NR G TTQ+TA+F AIGEGFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAF+NCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQ+NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+T+GDFIQP GYMPWSGNFALETC Y EYGNRGPGA TNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540

Query: 541 QFTAGSFLQGKTWLPTTGGPYLLGLK 566
           Q+TAG FL G+ WLP TGGPYLLGLK
Sbjct: 541 QYTAGPFLMGRAWLPATGGPYLLGLK 564

BLAST of CSPI05G02360 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 460.7 bits (1184), Expect = 1.7e-129
Identity = 267/605 (44.13%), Postives = 363/605 (60.00%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA  +LSP+MKAV  IC + + +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA--NLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIE-----AANNRSIKMGVED 120
           TL        S+DP + +KA ++AT DE+ K  N T    +      + NN+++   ++ 
Sbjct: 61  TL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LDY 120

Query: 121 CRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLR 180
           C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  LR
Sbjct: 121 CKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK-----------------------A 240
           + + +G+  +  LT+NA+ I   V   +A    ++                        A
Sbjct: 181 KAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVA 240

Query: 241 QPSG--RRLLGTTEVDNDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDGS 300
            P G  RRLL   ++D  G PTW++GADRKL+A         RGGGA+V+ N VVAKDGS
Sbjct: 241 DPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGS 300

Query: 301 GQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKS 360
           GQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   NIFM+GDG +KT+++ N+S
Sbjct: 301 GQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRS 360

Query: 361 --NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGY 420
                G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGY
Sbjct: 361 VALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGY 420

Query: 421 QDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPK 480
           QDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR+    Q NTVTA+G     
Sbjct: 421 QDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGL 480

Query: 481 EVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWS 540
            +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + MGD I+P G+  W 
Sbjct: 481 GMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWD 540

Query: 541 GNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGP 565
           G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L    W+     P
Sbjct: 541 GESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVP 588

BLAST of CSPI05G02360 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 451.4 bits (1160), Expect = 1.0e-126
Identity = 227/564 (40.25%), Postives = 351/564 (62.23%), Query Frame = 0

Query: 4   KVVLPGISLLLVVGVALAVVAIVNSNKSSDA-TEDLSPKMKAVSTICSTANYQEECQNTL 63
           + V+  IS +L++ + +AV   V+ NKS +A  E+++  +KA+  +C+  +Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E VK A  AT+ ++      +   +IE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 NGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL      +  +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIYKEYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGSFLQGKTWLPTTGGPYLLGLKS 567
              ++QG  W+P  G PY+LGL S
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of CSPI05G02360 vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-126
Identity = 266/613 (43.39%), Postives = 367/613 (59.87%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA  +LSP+MKAV  IC   + +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYI--NKNGDA--NLSPQMKAVRGICEATSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAIVATIDEVKKGYNL---TDGFLIE--AANNRSIKMGVED 120
           TL        SDDP + +KA ++AT D + +  N    T+G L    + NN+++   ++ 
Sbjct: 61  TL----EPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAV---LDY 120

Query: 121 CRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLR 180
           C+ +  +A++ L      +GE DL+    ++  +K WLT V +YQ  CLD +EE D  LR
Sbjct: 121 CKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK------------------------ 240
           + + +G+  +  LTSNA+ I   V   +A   L+++                        
Sbjct: 181 KTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGT 240

Query: 241 ---------AQPSG--RRLLGTTEVDNDGYPTWLTGADRKLLASRG-----GGAKVKPNA 300
                    A P G  RRLL   ++D  G PTW++GADRKL+   G     GGA+++   
Sbjct: 241 PPVADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATF 300

Query: 301 VVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKT 360
           VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY+E V I K   NIFM+GDG +KT
Sbjct: 301 VVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKT 360

Query: 361 IVTGNKSNR--GGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFF 420
           +++ N+S +   G TT  + T     EGF+ K +GF+NTAGP GHQAVA+RV  DR+  F
Sbjct: 361 VISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIF 420

Query: 421 NCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTA 480
           NCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S TVIQNSLI+VR+    Q NTVTA
Sbjct: 421 NCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTA 480

Query: 481 NGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQP 540
           +G      +  G+V+ NCRIVP++KL AER  + ++LGRPWK+++ TVI+ S +GD I+P
Sbjct: 481 DGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRP 540

Query: 541 VGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVI-GRNEAMQFTAGSFLQGKT 565
            G+  W G    ++C Y+EY NRGPGA+TNRRV W  VK+     E   FT  ++L    
Sbjct: 541 EGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPIN 595

BLAST of CSPI05G02360 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-126
Identity = 223/558 (39.96%), Postives = 344/558 (61.65%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNK---SSDATEDLSPKMKAVSTICSTANYQEECQNTLSNAA 69
           IS +L++ + +AV   V+ NK    S    +++  +KAV  +C+  +Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAIVATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T+ ++      +   ++E   +   +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQ-TIMELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAG 189
           L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRG 249
           +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RKLL +  
Sbjct: 200 ELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRKLLQAAA 259

Query: 250 GGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIF 309
             + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+YKEYV + K M ++ 
Sbjct: 260 AYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLV 319

Query: 310 MYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQ 369
             GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RVQ
Sbjct: 320 FIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQ 379

Query: 370 SDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDN 429
           SD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  V QN  ++VR+P+ N
Sbjct: 380 SDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPN 439

Query: 430 QQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTM 489
           Q   +TA+GR DP+E +G V   C I  E    A +     +LGRPWKEY+RT+IM + +
Sbjct: 440 QACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFI 499

Query: 490 GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSF 549
            DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++FT   +
Sbjct: 500 PDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQY 559

Query: 550 LQGKTWLPTTGGPYLLGL 565
           +QG  W+P  G PY  GL
Sbjct: 560 IQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02360 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-126
Identity = 255/598 (42.64%), Postives = 364/598 (60.87%), Query Frame = 0

Query: 4   KVVLPGISLLLV----VGVALAVVAIVN---------SNKSSDA---TEDLSPKM----- 63
           +++L  +S+L+V    +G A A V   N         +NKS D+   +E  SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSTANYQEECQNTLSN-AAHNASSDDPKEYVKAAIVATIDEVKKG 123
                    K + T+C++  Y+  CQNTL N    +    DP+  +K+AIVA  D++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFLIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNW 183
           +      L     N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRV---LSLKTENKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTSVISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASF-----G 243
           L++V+SYQ++C+DG EE   +L+ +++   N +  LTSN+LA++ ++   L+S       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKT 303
           L L+A+ S +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAK--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 ISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGF 363
           I+AAL A P   +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALET 543
           HNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 565
             Y EY N GP   T  RV+W G +V+ + EAM+FT G FLQG+ W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O807222.4e-12844.13Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q3E8Z81.5e-12540.25Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX862.5e-12539.96Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY62.5e-12542.64Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q5MFV82.5e-12543.39Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7U9J80.0e+0095.59Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR270.0e+0095.41Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A5A7U7W41.6e-27683.04Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
A0A0A0KLN28.0e-27682.51Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A1S3CR401.8e-27583.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0050275.10.0e+0095.59pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.10.0e+0095.41PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875878.14.9e-28887.43pectinesterase-like [Benincasa hispida][more]
KAA0050276.13.3e-27683.04pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [... [more]
XP_038875879.15.7e-27683.04LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G47030.11.7e-12944.13Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.11.0e-12640.25Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47040.11.8e-12643.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.11.8e-12639.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.8e-12642.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..195
e-value: 2.5E-32
score: 123.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..195
e-value: 1.3E-23
score: 84.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 19..197
e-value: 1.9E-18
score: 64.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 254..549
e-value: 7.3E-131
score: 435.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 42..200
e-value: 3.4E-29
score: 103.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..199
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 248..564
e-value: 2.1E-112
score: 377.6
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 4..564
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..564
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 7.1942E-47
score: 159.526
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 398..407
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 251..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02360.1CSPI05G02360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity