CSPI05G02340 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G02340
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr5: 3030412 .. 3032973 (+)
RNA-Seq ExpressionCSPI05G02340
SyntenyCSPI05G02340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCACCCTCCTCCATTCAAATCACTACAAAAACCCAACAAAAAAAAAGGGTAAAAAAAGGAAACACATAAATCAAAACCCAAACCTTTTGTTTTCCCATCTCTGTATGGTGGGAAAACTTTCTTGCATTAAGGTTTTGATTTTTGAAGGAAAAAAAATAGCAAGGTAAAAGAAAAAAGAAAAAAATGGTTGGGAAGGTGGTCATGTCGGGGATATCCCTTATTCTTGTAGTCGGTGTAGCTCTCGCGGTTGTGGCTATAGTAAACAAGAGCGATAGTTCTGATGAAACTGCAAATTTATCTCCAAAAATGAAGGCTGTCGCATCTATTTGTTCTAATGCCGATTACCAACCAGAATGTCAAACTACACTTGGTAGTGTTGGAAATAATTCGTCGGTAGAGGACCCGAAAGAGTTCATTAAAGCCGCGATTACGTCTACCATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAACAATGCTACAATTAAAATGAGTGTTGATGATTGTAAGGATTTATTACAATCTGCTATTGATGAGTTGCATGCGTCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTATCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAGCTTAAACAAAAGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACTAGTAATGCTCTTGCTATTGTTGGTGCTGTTTCTGATATTCTCAAAGAACTTGGCTTGCAATTGAAGGTAATTATGATATATCTCACTTCAAACTTTCAAATATACCAAAATTTCAACCTCTATCCATATTAATACATATCAATAAACTTTTATCACTTTCTATTGATACTAATAGACACAATTAAACTTTTATCACTTTCTATTAAATTCGATGTTAGTTGTCATTGTTGATAGATATGATAGTAGTCTATCAAGGTTTATAAATATCTATCATTGATTAGACATTGGCATTTTGCTATATCAATGAAGACTCTTTTATCAATATTTGCTACACCGTGACTCATATCATTTATTATTGATAGACTTTGAAAGATTTTGCTATATCTGCAATTTTTTAAAATGTTCTATATATTATTGACAGGTCCAACCGAGTGGTCGTAGGCTACTTGGAACAACAGAAGTAGATAGTGACTCGTTCCCGACATGGTTGACGGGATCAGATAGGAAGTTGTTGGCAGCAAAAAGAGGTGGTGTTCGAGTAAAACCGAACGTAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGGTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGGTCTATAATGAACCCATCATTGTCACTAAAGACATGAAAAATATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACTGGTCGCAAAAGCAATCGTGACGGAATCACCACCCAAAACACCGCATCTTTTGGTAAGTCTTCACAAAAATCACGTGGTTGAGTGATATAATATTAAATTTTCCTTTCACTATCAACTTAAACATTTTGGGTAATGGATGATTAAGAATACAAATTTACCTCTTGCAGCTGCCATTGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCTGAAGGCCATCAAGCAGTAGCTCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACCCACCGTCAATTCTACCGCAATTGTGTCATATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGTCGTCCCATGGACAACCAACAAAACACTGTTACGGCCAATGGTCGTGCAGATAAAAAGGAAATCTCTGGATTGGTTATCCATAACTGCAGAATCGTCCCAGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGACCATGGAAGGAATATGCTAGAACAGTGATCATGGAGTCGACATTGGGTGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGAATTTTGCATTGGCAACTTGTTCGTATTTCGAGTATGGGAACAGAGGGCCTGGAGCTAACACTAATAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAAAGCTTGGCTTCCAGGCACCGGTGGACCTTACCTCTTAGGGCTCAAGAATTAAAAACATTGTATTTTCATTCTTTTGAATCTCATCTTGGAGCCATGGTTTGCGCTCCATATTCAATATAGTGATTTCATATTCATTATTTACTTTCAAATTATATATCATGGTGGTTACAAGGTGCCACCCATTCAAAGAATGCTTAACACAATGCATCCCAAAAAAATGATAATTAAAGACCCGAATGAGCCTAGCTAGGAGATGGTC

mRNA sequence

ATCACCCTCCTCCATTCAAATCACTACAAAAACCCAACAAAAAAAAAGGGTAAAAAAAGGAAACACATAAATCAAAACCCAAACCTTTTGTTTTCCCATCTCTGTATGGTGGGAAAACTTTCTTGCATTAAGGTTTTGATTTTTGAAGGAAAAAAAATAGCAAGGTAAAAGAAAAAAGAAAAAAATGGTTGGGAAGGTGGTCATGTCGGGGATATCCCTTATTCTTGTAGTCGGTGTAGCTCTCGCGGTTGTGGCTATAGTAAACAAGAGCGATAGTTCTGATGAAACTGCAAATTTATCTCCAAAAATGAAGGCTGTCGCATCTATTTGTTCTAATGCCGATTACCAACCAGAATGTCAAACTACACTTGGTAGTGTTGGAAATAATTCGTCGGTAGAGGACCCGAAAGAGTTCATTAAAGCCGCGATTACGTCTACCATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAACAATGCTACAATTAAAATGAGTGTTGATGATTGTAAGGATTTATTACAATCTGCTATTGATGAGTTGCATGCGTCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTATCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAGCTTAAACAAAAGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACTAGTAATGCTCTTGCTATTGTTGGTGCTGTTTCTGATATTCTCAAAGAACTTGGCTTGCAATTGAAGGTCCAACCGAGTGGTCGTAGGCTACTTGGAACAACAGAAGTAGATAGTGACTCGTTCCCGACATGGTTGACGGGATCAGATAGGAAGTTGTTGGCAGCAAAAAGAGGTGGTGTTCGAGTAAAACCGAACGTAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGGTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGGTCTATAATGAACCCATCATTGTCACTAAAGACATGAAAAATATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACTGGTCGCAAAAGCAATCGTGACGGAATCACCACCCAAAACACCGCATCTTTTGCTGCCATTGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCTGAAGGCCATCAAGCAGTAGCTCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACCCACCGTCAATTCTACCGCAATTGTGTCATATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGTCGTCCCATGGACAACCAACAAAACACTGTTACGGCCAATGGTCGTGCAGATAAAAAGGAAATCTCTGGATTGGTTATCCATAACTGCAGAATCGTCCCAGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGACCATGGAAGGAATATGCTAGAACAGTGATCATGGAGTCGACATTGGGTGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGAATTTTGCATTGGCAACTTGTTCGTATTTCGAGTATGGGAACAGAGGGCCTGGAGCTAACACTAATAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAAAGCTTGGCTTCCAGGCACCGGTGGACCTTACCTCTTAGGGCTCAAGAATTAAAAACATTGTATTTTCATTCTTTTGAATCTCATCTTGGAGCCATGGTTTGCGCTCCATATTCAATATAGTGATTTCATATTCATTATTTACTTTCAAATTATATATCATGGTGGTTACAAGGTGCCACCCATTCAAAGAATGCTTAACACAATGCATCCCAAAAAAATGATAATTAAAGACCCGAATGAGCCTAGCTAGGAGATGGTC

Coding sequence (CDS)

ATGGTTGGGAAGGTGGTCATGTCGGGGATATCCCTTATTCTTGTAGTCGGTGTAGCTCTCGCGGTTGTGGCTATAGTAAACAAGAGCGATAGTTCTGATGAAACTGCAAATTTATCTCCAAAAATGAAGGCTGTCGCATCTATTTGTTCTAATGCCGATTACCAACCAGAATGTCAAACTACACTTGGTAGTGTTGGAAATAATTCGTCGGTAGAGGACCCGAAAGAGTTCATTAAAGCCGCGATTACGTCTACCATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAACAATGCTACAATTAAAATGAGTGTTGATGATTGTAAGGATTTATTACAATCTGCTATTGATGAGTTGCATGCGTCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTATCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAGCTTAAACAAAAGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACTAGTAATGCTCTTGCTATTGTTGGTGCTGTTTCTGATATTCTCAAAGAACTTGGCTTGCAATTGAAGGTCCAACCGAGTGGTCGTAGGCTACTTGGAACAACAGAAGTAGATAGTGACTCGTTCCCGACATGGTTGACGGGATCAGATAGGAAGTTGTTGGCAGCAAAAAGAGGTGGTGTTCGAGTAAAACCGAACGTAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGGTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGGTCTATAATGAACCCATCATTGTCACTAAAGACATGAAAAATATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACTGGTCGCAAAAGCAATCGTGACGGAATCACCACCCAAAACACCGCATCTTTTGCTGCCATTGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCTGAAGGCCATCAAGCAGTAGCTCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACCCACCGTCAATTCTACCGCAATTGTGTCATATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGTCGTCCCATGGACAACCAACAAAACACTGTTACGGCCAATGGTCGTGCAGATAAAAAGGAAATCTCTGGATTGGTTATCCATAACTGCAGAATCGTCCCAGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGACCATGGAAGGAATATGCTAGAACAGTGATCATGGAGTCGACATTGGGTGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGAATTTTGCATTGGCAACTTGTTCGTATTTCGAGTATGGGAACAGAGGGCCTGGAGCTAACACTAATAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAAAGCTTGGCTTCCAGGCACCGGTGGACCTTACCTCTTAGGGCTCAAGAATTAA

Protein sequence

MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGLKN*
Homology
BLAST of CSPI05G02340 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.3e-125
Identity = 228/561 (40.64%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 5   VVMSGISLILVVGVALAVVAIVNKSD-SSDETANLSPKMKAVASICSNADYQPECQTTLG 64
           + +S + LI +V VA+ V   +NK D  S   A ++  +KAV  +C+  DY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  SVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSA 124
            + N  +  DP E +K A   T++++      +  +M E   ++  +M++D CK+L+  A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLD 184
           +DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLA 244
            A +LT N LAI+  +S+ + ++ +      + RRLL      ++ FP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 AKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMK 304
           A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y E + V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DGITT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME 484
           + NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTA 544
           + + DF+QP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + ++FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLPGTGGPYLLGL 565
             ++ G  W+PG G PY  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02340 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 7.3e-125
Identity = 228/563 (40.50%), Postives = 349/563 (61.99%), Query Frame = 0

Query: 4   KVVMSGISLILVVGVALAVV--AIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 63
           + V+  IS +L++ + +AV     VNKSD++ +   ++  +KA+  +C+  DY+  C+ T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-EEITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 123
           L     ++S  DP E +K A  +T++++      +   M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 183
            AI EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 243
           L  A +LT N LA+V  +S+ L ++ +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKD 303
           L A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++K G+Y E + V + 
Sbjct: 255 LNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAV 363
           M ++   GDGP KT+++G KS +DGITT  TA+ A +G+ F+ K++ F NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 483
           +P+ NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQF 543
           M + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E ++F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLPGTGGPYLLGL 565
           T   ++ G AW+PG G PY+LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI05G02340 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.3e-123
Identity = 245/593 (41.32%), Postives = 357/593 (60.20%), Query Frame = 0

Query: 4   KVVMSGISLILV----VGVALAVVAIVNKSDSSDETAN------------LSPKM----- 63
           ++++  +S+++V    +G A A V   NK+    +T N             SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVASICSNADYQPECQTTL-GSVGNNSSVEDPKEFIKAAITSTIEEMKKG 123
                    K + ++C++  Y+P CQ TL      ++   DP+  +K+AI +  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNW 183
           +    +L  E   N   K ++  CK L+  A +EL  S   + D +++     + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKV 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L  +    KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAAL 303
           +        ++  ++D   +WL+  +R++L A      +KPN  VAKDGSG + TI AAL
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRMLKAV-DVKALKPNATVAKDGSGNFTTINAAL 323

Query: 304 AAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNT 363
            A P   +GRY IY+K G+Y+E +I+ K   N+ M GDG +KTIVTG KS+   I T  T
Sbjct: 324 KAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLT 383

Query: 364 ASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQF 423
           A+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+
Sbjct: 384 ATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQY 443

Query: 424 YRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRI 483
           YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHNC +
Sbjct: 444 YRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTV 503

Query: 484 VPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFE 543
            P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA+ T SY E
Sbjct: 504 APNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAE 563

Query: 544 YGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           Y N GP   T  RV+W G RV+ + EAM+FT GPFL G+ W+   G P  LGL
Sbjct: 564 YKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of CSPI05G02340 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.8e-122
Identity = 257/602 (42.69%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           M+GKVV+S  S++L+VGVA+ VVA +NK+      ANLSP+MKAV  IC +   +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNAT--IKMSVDDCKD 120
           TL  V +    EDP + IKA + +T +E+ K  N T    V   ++ +   K  +D CK 
Sbjct: 61  TLEPVKS----EDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +   A+++L      +G+ DL     +I  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVGAVSDILKELGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   + ++  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVDSDSFPTWLTGSDRKLLA--------AKRGGVRVKPNVVVAKDGSGQY 300
           G  RRLL   ++D    PTW++G+DRKL+A         + GG RV+ N VVAKDGSGQ+
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKS--N 360
           KT+  A+ A P+  +GR +IY+KAG+Y E +I+ K   NIFM+GDG RKT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEIS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLL 565
              +C Y EY NRGPGA  NRRV W  V      E   FTA  +L    W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of CSPI05G02340 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 434.1 bits (1115), Expect = 2.4e-120
Identity = 228/524 (43.51%), Postives = 334/524 (63.74%), Query Frame = 0

Query: 43  KAVASICSNADYQPECQTTL-GSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMV 102
           K + ++CS+  Y   C+ TL        ++++P  F+K+AI +  E++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 103 EAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQ 162
               N   K +++ CK L++ A +E  AS + +   ++++ E  + D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLG 222
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L  +   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV---MKVVE--RHLL- 286

Query: 223 TTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKG 282
                 D  P+W++  DR++L A      +KPN  VAKDGSG + TI  AL A P+  +G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAV-DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEG 342
           RY+IYVK G+Y+E + V K   N+ M GDG +KTIVTG KS+   I T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGT 402
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFEYGNRGPGAN 522
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   +FA+ T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           T  RV+W G +VI + EA+ +T GPFL G  W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of CSPI05G02340 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLGKAWLPGTGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CSPI05G02340 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.4e-303
Identity = 519/566 (91.70%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MV KVVM+ ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLG+AWLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CSPI05G02340 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.2e-302
Identity = 514/557 (92.28%), Postives = 540/557 (96.95%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTTLGSVGNNS 69
           ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQTTLG+VG+NS
Sbjct: 3   VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNS 62

Query: 70  SVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHA 129
           SV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLLQSAIDELHA
Sbjct: 63  SVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHA 122

Query: 130 SYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLT 189
           SYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ GLDVA KLT
Sbjct: 123 SYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLT 182

Query: 190 SNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGV 249
           SNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D FPTWLTG+DRKLLAAKRGGV
Sbjct: 183 SNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRGGV 242

Query: 250 RVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYG 309
           RVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTKDMKN+FMYG
Sbjct: 243 RVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYG 302

Query: 310 DGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR 369
           DGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR
Sbjct: 303 DGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR 362

Query: 370 SAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN 429
           SAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN
Sbjct: 363 SAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN 422

Query: 430 TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDF 489
           TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME+TLGDF
Sbjct: 423 TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDF 482

Query: 490 IQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLG 549
           IQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQFTAGPFLLG
Sbjct: 483 IQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLG 542

Query: 550 KAWLPGTGGPYLLGLKN 567
           +AWLP TGGPYLLGLKN
Sbjct: 543 RAWLPATGGPYLLGLKN 559

BLAST of CSPI05G02340 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.0e-275
Identity = 472/567 (83.25%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60
           MV KV++ GISLILVVGVALAVVAIV+ + SSD  T +LSPKMKAV++IC+NA+YQ ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120
            TL +  +N+S +DPKE+++AAI STIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240
           DGL  AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEV  D +PTWLTG+DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+YNE + +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 567
           QFTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02340 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.3e-275
Identity = 471/567 (83.07%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60
           MV KV++ GI+LILVVGVALAVVAIV+ + SSD  T +LSPKMKAV++IC+NA+YQ ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120
            TL +  +N+S +DPKE+++AAI STIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240
           DGL  AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEV  D +PTWLTG+DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+YNE + +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 567
           QFTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02340 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLGKAWLPGTGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CSPI05G02340 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.1e-303
Identity = 519/566 (91.70%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MV KVVM+ ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLG+AWLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CSPI05G02340 vs. NCBI nr
Match: XP_008466382.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1047.3 bits (2707), Expect = 4.6e-302
Identity = 514/557 (92.28%), Postives = 540/557 (96.95%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTTLGSVGNNS 69
           ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQTTLG+VG+NS
Sbjct: 3   VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNS 62

Query: 70  SVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHA 129
           SV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLLQSAIDELHA
Sbjct: 63  SVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHA 122

Query: 130 SYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLT 189
           SYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ GLDVA KLT
Sbjct: 123 SYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLT 182

Query: 190 SNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGV 249
           SNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D FPTWLTG+DRKLLAAKRGGV
Sbjct: 183 SNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRGGV 242

Query: 250 RVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYG 309
           RVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTKDMKN+FMYG
Sbjct: 243 RVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYG 302

Query: 310 DGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR 369
           DGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR
Sbjct: 303 DGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDR 362

Query: 370 SAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN 429
           SAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN
Sbjct: 363 SAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQN 422

Query: 430 TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDF 489
           TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME+TLGDF
Sbjct: 423 TVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDF 482

Query: 490 IQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLG 549
           IQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQFTAGPFLLG
Sbjct: 483 IQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLG 542

Query: 550 KAWLPGTGGPYLLGLKN 567
           +AWLP TGGPYLLGLKN
Sbjct: 543 RAWLPATGGPYLLGLKN 559

BLAST of CSPI05G02340 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.5e-289
Identity = 494/566 (87.28%), Postives = 536/566 (94.70%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MVGKVV+SGISLILVVGVALAVVA+V K+++SD   + SPKMKAV++ICS  DYQ ECQ 
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLS-SPKMKAVSTICSTTDYQQECQN 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TL +V  N+SV+DPKE+ KAA+ +TI+E+ KGYNLT +LMVEAANNA+IKMSVDDCKDLL
Sbjct: 61  TLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVAGKLTSNALAIVGAVSDILK  GLQLKVQPSGRRLL TTE+ SD FPTW++G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLA++ GG RVKPN VVAKDGSGQYK+IGAALAAYPKAL+GRYVIYVKAG+Y+E I VTK
Sbjct: 241 LLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRK+NRDGITTQNTASF+AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAF+NCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQ+NTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YARTV
Sbjct: 421 RRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQP GYMPWSGNFAL TCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEA+Q
Sbjct: 481 IMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           +TAGPFL+G+AWLP TGGPYLLGLK+
Sbjct: 541 YTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of CSPI05G02340 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 958.7 bits (2477), Expect = 2.2e-275
Identity = 472/567 (83.25%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60
           MV KV++ GISLILVVGVALAVVAIV+ + SSD  T +LSPKMKAV++IC+NA+YQ ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120
            TL +  +N+S +DPKE+++AAI STIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240
           DGL  AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEV  D +PTWLTG+DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+YNE + +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 567
           QFTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of CSPI05G02340 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 5.2e-126
Identity = 228/561 (40.64%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 5   VVMSGISLILVVGVALAVVAIVNKSD-SSDETANLSPKMKAVASICSNADYQPECQTTLG 64
           + +S + LI +V VA+ V   +NK D  S   A ++  +KAV  +C+  DY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  SVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSA 124
            + N  +  DP E +K A   T++++      +  +M E   ++  +M++D CK+L+  A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLD 184
           +DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLA 244
            A +LT N LAI+  +S+ + ++ +      + RRLL      ++ FP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 AKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMK 304
           A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y E + V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DGITT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME 484
           + NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTA 544
           + + DF+QP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + ++FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLPGTGGPYLLGL 565
             ++ G  W+PG G PY  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02340 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 5.2e-126
Identity = 228/563 (40.50%), Postives = 349/563 (61.99%), Query Frame = 0

Query: 4   KVVMSGISLILVVGVALAVV--AIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 63
           + V+  IS +L++ + +AV     VNKSD++ +   ++  +KA+  +C+  DY+  C+ T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-EEITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 123
           L     ++S  DP E +K A  +T++++      +   M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 183
            AI EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 243
           L  A +LT N LA+V  +S+ L ++ +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKD 303
           L A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++K G+Y E + V + 
Sbjct: 255 LNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAV 363
           M ++   GDGP KT+++G KS +DGITT  TA+ A +G+ F+ K++ F NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 483
           +P+ NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQF 543
           M + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E ++F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLPGTGGPYLLGL 565
           T   ++ G AW+PG G PY+LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI05G02340 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 444.1 bits (1141), Expect = 1.7e-124
Identity = 245/593 (41.32%), Postives = 357/593 (60.20%), Query Frame = 0

Query: 4   KVVMSGISLILV----VGVALAVVAIVNKSDSSDETAN------------LSPKM----- 63
           ++++  +S+++V    +G A A V   NK+    +T N             SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVASICSNADYQPECQTTL-GSVGNNSSVEDPKEFIKAAITSTIEEMKKG 123
                    K + ++C++  Y+P CQ TL      ++   DP+  +K+AI +  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNW 183
           +    +L  E   N   K ++  CK L+  A +EL  S   + D +++     + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKV 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L  +    KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAAL 303
           +        ++  ++D   +WL+  +R++L A      +KPN  VAKDGSG + TI AAL
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRMLKAV-DVKALKPNATVAKDGSGNFTTINAAL 323

Query: 304 AAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNT 363
            A P   +GRY IY+K G+Y+E +I+ K   N+ M GDG +KTIVTG KS+   I T  T
Sbjct: 324 KAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLT 383

Query: 364 ASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQF 423
           A+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+
Sbjct: 384 ATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQY 443

Query: 424 YRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRI 483
           YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHNC +
Sbjct: 444 YRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTV 503

Query: 484 VPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFE 543
            P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA+ T SY E
Sbjct: 504 APNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAE 563

Query: 544 YGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           Y N GP   T  RV+W G RV+ + EAM+FT GPFL G+ W+   G P  LGL
Sbjct: 564 YKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of CSPI05G02340 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.5 bits (1129), Expect = 4.1e-123
Identity = 257/602 (42.69%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           M+GKVV+S  S++L+VGVA+ VVA +NK+      ANLSP+MKAV  IC +   +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNAT--IKMSVDDCKD 120
           TL  V +    EDP + IKA + +T +E+ K  N T    V   ++ +   K  +D CK 
Sbjct: 61  TLEPVKS----EDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +   A+++L      +G+ DL     +I  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVGAVSDILKELGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   + ++  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVDSDSFPTWLTGSDRKLLA--------AKRGGVRVKPNVVVAKDGSGQY 300
           G  RRLL   ++D    PTW++G+DRKL+A         + GG RV+ N VVAKDGSGQ+
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKS--N 360
           KT+  A+ A P+  +GR +IY+KAG+Y E +I+ K   NIFM+GDG RKT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEIS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLL 565
              +C Y EY NRGPGA  NRRV W  V      E   FTA  +L    W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of CSPI05G02340 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.1 bits (1115), Expect = 1.7e-121
Identity = 228/524 (43.51%), Postives = 334/524 (63.74%), Query Frame = 0

Query: 43  KAVASICSNADYQPECQTTL-GSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMV 102
           K + ++CS+  Y   C+ TL        ++++P  F+K+AI +  E++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 103 EAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQ 162
               N   K +++ CK L++ A +E  AS + +   ++++ E  + D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLG 222
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L  +   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV---MKVVE--RHLL- 286

Query: 223 TTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKG 282
                 D  P+W++  DR++L A      +KPN  VAKDGSG + TI  AL A P+  +G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAV-DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEG 342
           RY+IYVK G+Y+E + V K   N+ M GDG +KTIVTG KS+   I T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGT 402
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFEYGNRGPGAN 522
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   +FA+ T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           T  RV+W G +VI + EA+ +T GPFL G  W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX867.3e-12540.64Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z87.3e-12540.50Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY62.3e-12341.32Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
O807225.8e-12242.69Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q7Y2012.4e-12043.51Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0KLN20.0e+00100.00Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A5A7U7W43.4e-30391.70Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
A0A1S3CR402.2e-30292.28Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1[more]
A0A5A7U9J81.0e-27583.25Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR272.3e-27583.07Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KGN49779.10.0e+00100.00hypothetical protein Csa_017783 [Cucumis sativus][more]
KAA0050276.17.1e-30391.70pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [... [more]
XP_008466382.24.6e-30292.28PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875879.14.5e-28987.28LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KAA0050275.12.2e-27583.25pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
Match NameE-valueIdentityDescription
AT3G05610.15.2e-12640.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.15.2e-12640.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.7e-12441.32Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.14.1e-12342.69Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.11.7e-12143.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..195
e-value: 2.4E-33
score: 126.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..195
e-value: 8.3E-24
score: 84.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 18..196
e-value: 1.3E-20
score: 71.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 254..549
e-value: 7.2E-130
score: 432.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 248..564
e-value: 3.7E-109
score: 366.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 42..199
e-value: 6.1E-29
score: 102.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..199
NoneNo IPR availablePANTHERPTHR31707:SF292PECTINESTERASEcoord: 1..564
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 1..564
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 8.49054E-47
score: 159.141
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 398..407
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 251..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02340.1CSPI05G02340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity