CSPI05G01520 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G01520
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calmodulin-like
Location	Chr5: 2125071 .. 2125601 (+)
RNA-Seq Expression	CSPI05G01520
Synteny	CSPI05G01520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATATCTCAAATCTTCAAGTTTCCAAAGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAACATGAAAATAATGACGAAGGTGTTCCACTTAAAGCTTTCAATGGCATTCCTCCAGCTTCAACTAAAATCAACAACAAGCAAGCAATGGCAACGGAAATTATCCTGAAACCTAGTAATCATCCTGTTGCACACTACCTTACATCAAATGCCTATATGATACCAAGTAAGCAATTGGGAGCTGGAAAAGCTCAAGTAACCACCACAAGTGTTCTTGATATAAAGGCTTCTAATATGCAATTTAGTCGAGAGCAAATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACCTAAGGGAGGTAACCAAAGCTTTTGCTTTTCTAGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATACGCTGATGCTAATAAAGATGGTCTTATTAGCAAGGATGAACTCGAAAAACTCGTTGATTATGCATATAAATTTTTCAAGAAACGATGA

mRNA sequence

ATGTATATCTCAAATCTTCAAGTTTCCAAAGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAACATGAAAATAATGACGAAGGTGTTCCACTTAAAGCTTTCAATGGCATTCCTCCAGCTTCAACTAAAATCAACAACAAGCAAGCAATGGCAACGGAAATTATCCTGAAACCTAGTAATCATCCTGTTGCACACTACCTTACATCAAATGCCTATATGATACCAAGTAAGCAATTGGGAGCTGGAAAAGCTCAAGTAACCACCACAAGTGTTCTTGATATAAAGGCTTCTAATATGCAATTTAGTCGAGAGCAAATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACCTAAGGGAGGTAACCAAAGCTTTTGCTTTTCTAGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATACGCTGATGCTAATAAAGATGGTCTTATTAGCAAGGATGAACTCGAAAAACTCGTTGATTATGCATATAAATTTTTCAAGAAACGATGA

Coding sequence (CDS)

ATGTATATCTCAAATCTTCAAGTTTCCAAAGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAACATGAAAATAATGACGAAGGTGTTCCACTTAAAGCTTTCAATGGCATTCCTCCAGCTTCAACTAAAATCAACAACAAGCAAGCAATGGCAACGGAAATTATCCTGAAACCTAGTAATCATCCTGTTGCACACTACCTTACATCAAATGCCTATATGATACCAAGTAAGCAATTGGGAGCTGGAAAAGCTCAAGTAACCACCACAAGTGTTCTTGATATAAAGGCTTCTAATATGCAATTTAGTCGAGAGCAAATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACCTAAGGGAGGTAACCAAAGCTTTTGCTTTTCTAGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATACGCTGATGCTAATAAAGATGGTCTTATTAGCAAGGATGAACTCGAAAAACTCGTTGATTATGCATATAAATTTTTCAAGAAACGATGA

Protein sequence

MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR*

Homology

[Close]

BLAST of CSPI05G01520 vs. ExPASy TrEMBL
Match: A0A0A0KIY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.0e-92
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
           HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFF KR
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CSPI05G01520 vs. ExPASy TrEMBL
Match: A0A0A0KLF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.3e-51
Identity = 117/176 (66.48%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           M+I  LQ+SKAKYEASKLMKK+E N EGV L AFN     ST+IN  + MAT+  LKP N
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATK-KLKP-N 87

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             VAH L+SNA +  + Q+  GKAQV    V D KASNMQFSREQIKEIFQY+DSD DGF
Sbjct: 88  DDVAHKLSSNAQLKSNNQI-RGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGF 147

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+REVTKAFA LG+I P  KAYHGMVYAD NKDGLI +DEL+KL++YA KF KK+
Sbjct: 148 LNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of CSPI05G01520 vs. ExPASy TrEMBL
Match: A0A0A0KMQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.3e-43
Identity = 101/176 (57.39%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           MYIS LQ S A YEAS L+K++E N + V +KAF+ +  +ST+I N++++    ILKPS+
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLK--ILKPSD 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             VAH L S A++ P+++ G GK + + T VLD K+SNM  SRE+IKEIFQYHD++NDGF
Sbjct: 61  -GVAHKLRSEAHIQPTQRTG-GKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN  E+TKAFAFLG++ P YKA +GMVYADAN+DGLIS+ EL+KL+DYA K  KK+
Sbjct: 121 LNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of CSPI05G01520 vs. ExPASy TrEMBL
Match: A0A6J1EP72 (calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 4.7e-41
Identity = 99/176 (56.25%), Postives = 121/176 (68.75%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           M+I  LQ S AKYEAS+++KK   N + V LKAFNGI  AST++NN Q+M  EI+    N
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQVNN-QSMVPEIL--RDN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             V    +SN +                     +KASNMQFSREQ+KEIF+YHDSD DGF
Sbjct: 61  KNVGPKFSSNTH---------------------LKASNMQFSREQMKEIFRYHDSDKDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+RE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KLVDYA KF KK+
Sbjct: 121 LNIRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of CSPI05G01520 vs. ExPASy TrEMBL
Match: A0A5A7VI66 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001550 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 2.6e-39
Identity = 94/176 (53.41%), Postives = 127/176 (72.16%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           MYIS LQ S A YEA   +K +E + + V +K FN +  +ST+I   Q +  +I+   S+
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDV-RSSTQI-GYQGIVPKIL--KSS 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             VAH L+  A++ P++Q G GK + +    LDIK+SNM FSR+QIKEIF+YHD++NDGF
Sbjct: 61  DDVAHKLSLEAHIQPTQQTG-GKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+ E+TKAFAFLG++ P YKA +GMVYADAN DGLIS+ EL+KL+DYA K  KK+
Sbjct: 121 LNIVELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of CSPI05G01520 vs. NCBI nr
Match: KAE8648235.1 (hypothetical protein Csa_018526 [Cucumis sativus])

HSP 1 Score: 290.4 bits (742), Expect = 1.0e-74
Identity = 147/150 (98.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
           HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYAD 151
           LNLREVTKAFAFLGTILPLYKAYHGM   D
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMTDYD 150

[Close]

BLAST of CSPI05G01520 vs. NCBI nr
Match: XP_022930012.1 (calmodulin-2/4-like [Cucurbita moschata])

HSP 1 Score: 177.6 bits (449), Expect = 9.7e-41
Identity = 99/176 (56.25%), Postives = 121/176 (68.75%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           M+I  LQ S AKYEAS+++KK   N + V LKAFNGI  AST++NN Q+M  EI+    N
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQVNN-QSMVPEIL--RDN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             V    +SN +                     +KASNMQFSREQ+KEIF+YHDSD DGF
Sbjct: 61  KNVGPKFSSNTH---------------------LKASNMQFSREQMKEIFRYHDSDKDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+RE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KLVDYA KF KK+
Sbjct: 121 LNIRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of CSPI05G01520 vs. NCBI nr
Match: XP_023530739.1 (calmodulin-2/4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-40
Identity = 98/176 (55.68%), Postives = 121/176 (68.75%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           M+I  LQ S AKYEAS+++KK+  N + V LKAFNGI  AST++NN Q M  EI+    N
Sbjct: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFNGIARASTQVNN-QGMVPEIL--RDN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             V    +SN +                     +KASNMQFSRE++KEIF+YHDSD DGF
Sbjct: 61  KNVGPKFSSNTH---------------------LKASNMQFSREKMKEIFRYHDSDKDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+RE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KLVDYA KF KK+
Sbjct: 121 LNIRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFMKKK 152

[Close]

BLAST of CSPI05G01520 vs. NCBI nr
Match: KAA0065465.1 (putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 171.8 bits (434), Expect = 5.3e-39
Identity = 94/176 (53.41%), Postives = 127/176 (72.16%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           MYIS LQ S A YEA   +K +E + + V +K FN +  +ST+I   Q +  +I+   S+
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDV-RSSTQI-GYQGIVPKIL--KSS 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             VAH L+  A++ P++Q G GK + +    LDIK+SNM FSR+QIKEIF+YHD++NDGF
Sbjct: 61  DDVAHKLSLEAHIQPTQQTG-GKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LN+ E+TKAFAFLG++ P YKA +GMVYADAN DGLIS+ EL+KL+DYA K  KK+
Sbjct: 121 LNIVELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of CSPI05G01520 vs. NCBI nr
Match: XP_022930019.1 (calcium-binding protein CML38-like [Cucurbita moschata])

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-38
Identity = 96/176 (54.55%), Postives = 117/176 (66.48%), Query Frame = 0


Query: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60
           M+I  LQ S AKYEAS+++KK   N + V LKAF+GI  AST++NN Q M  EI     N
Sbjct: 1   MFIPKLQASMAKYEASEMIKKRTRNGDNVQLKAFSGIARASTQVNN-QGMVPEIF--RDN 60

Query: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120
             V   L+SN                       +KASNMQFSRE +KEIF+YHDSD DGF
Sbjct: 61  KNVGPKLSSNT---------------------QLKASNMQFSREHLKEIFKYHDSDKDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFKKR 177
           LNLRE+TKAFAFLG+I+P  K  +GM YADA+KDGLIS+ EL+KL+DYA KF KK+
Sbjct: 121 LNLRELTKAFAFLGSIIPFEKGSYGMTYADADKDGLISEVELDKLIDYAQKFMKKK 152

The following BLAST results are available for this feature:

BLAST of CSPI05G01520 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI05G01520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIY4	2.0e-92	99.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0KLF3	1.3e-51	66.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0KMQ1	2.3e-43	57.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1	[more]
A0A6J1EP72	4.7e-41	56.25	calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1	[more]
A0A5A7VI66	2.6e-39	53.41	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of CSPI05G01520 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648235.1	1.0e-74	98.00	hypothetical protein Csa_018526 [Cucumis sativus]	[more]
XP_022930012.1	9.7e-41	56.25	calmodulin-2/4-like [Cucurbita moschata]	[more]
XP_023530739.1	2.2e-40	55.68	calmodulin-2/4-like [Cucurbita pepo subsp. pepo]	[more]
KAA0065465.1	5.3e-39	53.41	putative calcium-binding protein CML11 [Cucumis melo var. makuwa]	[more]
XP_022930019.1	1.2e-38	54.55	calcium-binding protein CML38-like [Cucurbita moschata]	[more]

BLAST of CSPI05G01520 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 41..171 e-value: 4.1E-11 score: 45.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 103..165 e-value: 4.5E-8 score: 33.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 101..136 score: 12.114401
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 114..126
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 150..162
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 97..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G01520.1	CSPI05G01520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding