CSPI05G01500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G01500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calmodulin-like
Location	Chr5: 2105155 .. 2105833 (+)
RNA-Seq Expression	CSPI05G01500
Synteny	CSPI05G01500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAAAATGGTGTTGAAGTTTGTGCCATCTTCTAAATCTTCATATAGAAACAAATACATGGATGCCATAACCAAACCAAACCAAACCATGCATATCCCCCAACTTCAAATGTCCAAGGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAGTATGAACAAAATGGTGAAGGTGTTATACTAAGTGCTTTCAACGATAATGCTCGAGGTTCGACTCAAATCAACACTAATCGAGAAATGGCGACAAAAAAGCTAAAACCTAACGATGATGTTGCGCATAAACTTAGTTCAAATGCTCAATTAAAATCAAATAATCAAATACGAGGAAAAGCTCAAGTACCCATCAAAAGAGTTTATGATACAAAGGCATCTAACATGCAATTTAGTCGAGAGCAGATAAAGGAAATTTTTCAATACTATGATAGCGACCGTGACGGTTTTCTCAACATAAGAGAGGTGACAAAAGCTTTTGCTCTTCTAGGATCAATTTTTCCATTCAACAAAGCCTACCATGGCATGGTATATACTGATACTAATAAAGATGGACTTATTGGTGAAGATGAACTCGACAAACTCATAAATTATGCGAATAAATTTATGAAGAAAAAATGAGTGTTGAGCAGCTATATTTACATATCCATTTTCTTGTATTAAGGTAAAATTGTGAGATTTGAAGTCCACCG

mRNA sequence

TGAAAATGGTGTTGAAGTTTGTGCCATCTTCTAAATCTTCATATAGAAACAAATACATGGATGCCATAACCAAACCAAACCAAACCATGCATATCCCCCAACTTCAAATGTCCAAGGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAGTATGAACAAAATGGTGAAGGTGTTATACTAAGTGCTTTCAACGATAATGCTCGAGGTTCGACTCAAATCAACACTAATCGAGAAATGGCGACAAAAAAGCTAAAACCTAACGATGATGTTGCGCATAAACTTAGTTCAAATGCTCAATTAAAATCAAATAATCAAATACGAGGAAAAGCTCAAGTACCCATCAAAAGAGTTTATGATACAAAGGCATCTAACATGCAATTTAGTCGAGAGCAGATAAAGGAAATTTTTCAATACTATGATAGCGACCGTGACGGTTTTCTCAACATAAGAGAGGTGACAAAAGCTTTTGCTCTTCTAGGATCAATTTTTCCATTCAACAAAGCCTACCATGGCATGGTATATACTGATACTAATAAAGATGGACTTATTGGTGAAGATGAACTCGACAAACTCATAAATTATGCGAATAAATTTATGAAGAAAAAATGAGTGTTGAGCAGCTATATTTACATATCCATTTTCTTGTATTAAGGTAAAATTGTGAGATTTGAAGTCCACCG

Coding sequence (CDS)

ATGGTGTTGAAGTTTGTGCCATCTTCTAAATCTTCATATAGAAACAAATACATGGATGCCATAACCAAACCAAACCAAACCATGCATATCCCCCAACTTCAAATGTCCAAGGCCAAGTATGAAGCAAGTAAGTTGATGAAGAAGTATGAACAAAATGGTGAAGGTGTTATACTAAGTGCTTTCAACGATAATGCTCGAGGTTCGACTCAAATCAACACTAATCGAGAAATGGCGACAAAAAAGCTAAAACCTAACGATGATGTTGCGCATAAACTTAGTTCAAATGCTCAATTAAAATCAAATAATCAAATACGAGGAAAAGCTCAAGTACCCATCAAAAGAGTTTATGATACAAAGGCATCTAACATGCAATTTAGTCGAGAGCAGATAAAGGAAATTTTTCAATACTATGATAGCGACCGTGACGGTTTTCTCAACATAAGAGAGGTGACAAAAGCTTTTGCTCTTCTAGGATCAATTTTTCCATTCAACAAAGCCTACCATGGCATGGTATATACTGATACTAATAAAGATGGACTTATTGGTGAAGATGAACTCGACAAACTCATAAATTATGCGAATAAATTTATGAAGAAAAAATGA

Protein sequence

MVLKFVPSSKSSYRNKYMDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK*

Homology

[Close]

BLAST of CSPI05G01500 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-05
Identity = 39/156 (25.00%), Postives = 66/156 (42.31%), Query Frame = 0


Query: 41  EASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLKS 100
           E  ++ + +++NG+G +           TQ+     + +  LKP+ D    L   A   S
Sbjct: 64  ELREIFRSFDRNGDGSL-----------TQLELGSLLRSLGLKPSTDELDSLIQRADTNS 123

Query: 101 NNQIRGK---AQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALL 160
           N  I      A V  + +YD       +S +QI+ +F  +D D +GF+   E+  + A L
Sbjct: 124 NGLIEFSEFVALVAPELLYD----RAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKL 183

Query: 161 GSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYA 194
           G      +    +   DT+ DG I   E  + I  A
Sbjct: 184 GHALTVKELTGMIKEADTDGDGRISFQEFSRAITAA 204

[Close]

BLAST of CSPI05G01500 vs. ExPASy Swiss-Prot
Match: Q9LQN4 (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 39/157 (24.84%), Postives = 68/157 (43.31%), Query Frame = 0


Query: 40  YEASKLMKKYEQNGEGVILSAFNDNARGS-TQINTNREMATKKLKPNDDVAHKLSSNAQL 99
           ++ SK + + + N    I  +F+ N  GS TQ+     +    +KP+ D    L   A  
Sbjct: 3   HKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADT 62

Query: 100 KSNNQIRGKAQVPI--KRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFAL 159
           KSN  +     V +    +         ++ EQ+  +F+ +D+D +GF+   E+  + A 
Sbjct: 63  KSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAK 122

Query: 160 LGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYA 194
           LG      +    +   D++ DG I   E  K IN A
Sbjct: 123 LGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

[Close]

BLAST of CSPI05G01500 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-04
Identity = 38/155 (24.52%), Postives = 71/155 (45.81%), Query Frame = 0


Query: 32  QLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHK 91
           QL+ S+ K +  +L ++++ NG+G +           TQ+     + +  L+P  D  H 
Sbjct: 17  QLRGSQLK-QLRELFRRFDMNGDGSL-----------TQLELAALLRSLGLRPTGDEVHA 76

Query: 92  LSSNAQLKSNNQIR-GKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREV 151
           L +      N  +   +    I  V  T+   +   + Q+ E+F+ +D D +GF++  E+
Sbjct: 77  LLAGMDANGNGSVEFDELAAAIAPVLTTQTHLV--DQAQLLEVFRAFDRDGNGFISAAEL 136

Query: 152 TKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDE 186
            ++ A LG    F +    M   DT+ DG+I   E
Sbjct: 137 ARSMARLGQPLTFEELTRMMRDADTDGDGVISFKE 157

[Close]

BLAST of CSPI05G01500 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 8.6e-04
Identity = 32/121 (26.45%), Postives = 57/121 (47.11%), Query Frame = 0


Query: 77  MATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKAS------------NMQ 136
           MAT+K KP+ + + K  S   LK N   + + + P      T  S            + Q
Sbjct: 1   MATQKEKPSSN-SFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQ 60

Query: 137 FSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGED 186
            S+E+++++F ++DSD DG ++  E+   F  +G       A   +   DT+ DG +G +
Sbjct: 61  ASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFE 120

[Close]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL
Match: A0A0A0KLF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.2e-106
Identity = 199/200 (99.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MVLKFVPSSKSSYRNKYMDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSA 60
           MVLKFVPSSKSSYRNKYMDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSA
Sbjct: 1   MVLKFVPSSKSSYRNKYMDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSA 60

Query: 61  FNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKA 120
           FNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKA
Sbjct: 61  FNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKA 120

Query: 121 SNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGL 180
           SNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVY DTNKDGL
Sbjct: 121 SNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDGL 180

Query: 181 IGEDELDKLINYANKFMKKK 201
           IGEDELDKLINYANKFMKKK
Sbjct: 181 IGEDELDKLINYANKFMKKK 200

[Close]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL
Match: A0A0A0KIY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 3.7e-50
Identity = 115/176 (65.34%), Postives = 133/176 (75.57%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATK-KLKP-N 87
           M+I  LQ+SKAKYEASKLMKK+E N EGV L AFN     ST+IN  + MAT+  LKP N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 88  DDVAHKLSSNAQLKSNNQI-RGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGF 147
             VAH L+SNA +  + Q+  GKAQV    V D KASNMQFSREQIKEIFQY+DSD DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 148 LNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
           LN+REVTKAFA LG+I P  KAYHGMVY D NKDGLI +DEL+KL++YA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL
Match: A0A0A0KMQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 6.1e-45
Identity = 103/173 (59.54%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M+I +LQ S A YEAS L+K+YE+NG+ V + AF D+ R STQI  NRE   K LKP+D 
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAF-DDVRSSTQIG-NRESVLKILKPSDG 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           VAHKL S A ++   +  GK +  I RV D K+SNM  SRE+IKEIFQY+D++ DGFLN 
Sbjct: 61  VAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNR 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
            E+TKAFA LGS+FPF KA +GMVY D N+DGLI E ELDKLI+YA+K +KKK
Sbjct: 121 MELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL
Match: A0A6J1EP72 (calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 2.2e-42
Identity = 100/173 (57.80%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M IP+LQ S AKYEAS+++KK  +NG+ V L AFN  AR STQ+N N+ M  + L+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQVN-NQSMVPEILRDNKN 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           V  K SSN  L                    KASNMQFSREQ+KEIF+Y+DSD+DGFLNI
Sbjct: 61  VGPKFSSNTHL--------------------KASNMQFSREQMKEIFRYHDSDKDGFLNI 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
           RE+TKAFA LGSI PF KA +GM Y D NKDGLI E ELDKL++YA KF+KKK
Sbjct: 121 RELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL
Match: A0A5A7VI66 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001550 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.4e-41
Identity = 98/173 (56.65%), Postives = 122/173 (70.52%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M+I +LQ S A YEA   +K YE++G+ V +  FND  R STQI   + +  K LK +DD
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFND-VRSSTQIG-YQGIVPKILKSSDD 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           VAHKLS  A ++   Q  GK +  I R  D K+SNM FSR+QIKEIF+Y+D++ DGFLNI
Sbjct: 61  VAHKLSLEAHIQPTQQTGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLNI 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
            E+TKAFA LGS+FPF KA +GMVY D N DGLI E ELDKLI+YA K +KKK
Sbjct: 121 VELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of CSPI05G01500 vs. NCBI nr
Match: KAE8648234.1 (hypothetical protein Csa_018399 [Cucumis sativus])

HSP 1 Score: 301.6 bits (771), Expect = 5.1e-78
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 18  MDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREM 77
           MDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREM
Sbjct: 1   MDAITKPNQTMHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREM 60

Query: 78  ATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYY 137
           ATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYY
Sbjct: 61  ATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYY 120

Query: 138 DSDRDGFLNIREVTKAFALLGSIFPFNKAYHGM 171
           DSDRDGFLNIREVTKAFALLGSIFPFNKAYHGM
Sbjct: 121 DSDRDGFLNIREVTKAFALLGSIFPFNKAYHGM 153

[Close]

BLAST of CSPI05G01500 vs. NCBI nr
Match: XP_022930012.1 (calmodulin-2/4-like [Cucurbita moschata])

HSP 1 Score: 182.2 bits (461), Expect = 4.5e-42
Identity = 100/173 (57.80%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M IP+LQ S AKYEAS+++KK  +NG+ V L AFN  AR STQ+N N+ M  + L+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQVN-NQSMVPEILRDNKN 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           V  K SSN  L                    KASNMQFSREQ+KEIF+Y+DSD+DGFLNI
Sbjct: 61  VGPKFSSNTHL--------------------KASNMQFSREQMKEIFRYHDSDKDGFLNI 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
           RE+TKAFA LGSI PF KA +GM Y D NKDGLI E ELDKL++YA KF+KKK
Sbjct: 121 RELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of CSPI05G01500 vs. NCBI nr
Match: XP_023530739.1 (calmodulin-2/4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 181.4 bits (459), Expect = 7.6e-42
Identity = 100/173 (57.80%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M IP+LQ S AKYEAS+++KK  +NG+ V L AFN  AR STQ+N N+ M  + L+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFNGIARASTQVN-NQGMVPEILRDNKN 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           V  K SSN  L                    KASNMQFSRE++KEIF+Y+DSD+DGFLNI
Sbjct: 61  VGPKFSSNTHL--------------------KASNMQFSREKMKEIFRYHDSDKDGFLNI 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
           RE+TKAFA LGSI PF KA +GM Y D NKDGLI E ELDKL++YA KFMKKK
Sbjct: 121 RELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFMKKK 152

[Close]

BLAST of CSPI05G01500 vs. NCBI nr
Match: KAA0065465.1 (putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 179.5 bits (454), Expect = 2.9e-41
Identity = 98/173 (56.65%), Postives = 122/173 (70.52%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M+I +LQ S A YEA   +K YE++G+ V +  FND  R STQI   + +  K LK +DD
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFND-VRSSTQIG-YQGIVPKILKSSDD 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           VAHKLS  A ++   Q  GK +  I R  D K+SNM FSR+QIKEIF+Y+D++ DGFLNI
Sbjct: 61  VAHKLSLEAHIQPTQQTGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLNI 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
            E+TKAFA LGS+FPF KA +GMVY D N DGLI E ELDKLI+YA K +KKK
Sbjct: 121 VELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of CSPI05G01500 vs. NCBI nr
Match: XP_022930019.1 (calcium-binding protein CML38-like [Cucurbita moschata])

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-40
Identity = 98/173 (56.65%), Postives = 119/173 (68.79%), Query Frame = 0


Query: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87
           M IP+LQ S AKYEAS+++KK  +NG+ V L AF+  AR STQ+N N+ M  +  + N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKRTRNGDNVQLKAFSGIARASTQVN-NQGMVPEIFRDNKN 60

Query: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147
           V  KLSSN QL                    KASNMQFSRE +KEIF+Y+DSD+DGFLN+
Sbjct: 61  VGPKLSSNTQL--------------------KASNMQFSREHLKEIFKYHDSDKDGFLNL 120

Query: 148 REVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYANKFMKKK 201
           RE+TKAFA LGSI PF K  +GM Y D +KDGLI E ELDKLI+YA KFMKKK
Sbjct: 121 RELTKAFAFLGSIIPFEKGSYGMTYADADKDGLISEVELDKLIDYAQKFMKKK 152

[Close]

BLAST of CSPI05G01500 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 48.1 bits (113), Expect = 9.4e-06
Identity = 39/157 (24.84%), Postives = 68/157 (43.31%), Query Frame = 0


Query: 40  YEASKLMKKYEQNGEGVILSAFNDNARGS-TQINTNREMATKKLKPNDDVAHKLSSNAQL 99
           ++ SK + + + N    I  +F+ N  GS TQ+     +    +KP+ D    L   A  
Sbjct: 3   HKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADT 62

Query: 100 KSNNQIRGKAQVPI--KRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFAL 159
           KSN  +     V +    +         ++ EQ+  +F+ +D+D +GF+   E+  + A 
Sbjct: 63  KSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAK 122

Query: 160 LGSIFPFNKAYHGMVYTDTNKDGLIGEDELDKLINYA 194
           LG      +    +   D++ DG I   E  K IN A
Sbjct: 123 LGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

[Close]

BLAST of CSPI05G01500 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 45.4 bits (106), Expect = 6.1e-05
Identity = 32/121 (26.45%), Postives = 57/121 (47.11%), Query Frame = 0


Query: 77  MATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKAS------------NMQ 136
           MAT+K KP+ + + K  S   LK N   + + + P      T  S            + Q
Sbjct: 1   MATQKEKPSSN-SFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQ 60

Query: 137 FSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGED 186
            S+E+++++F ++DSD DG ++  E+   F  +G       A   +   DT+ DG +G +
Sbjct: 61  ASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFE 120

[Close]

BLAST of CSPI05G01500 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 43.1 bits (100), Expect = 3.0e-04
Identity = 35/135 (25.93%), Postives = 61/135 (45.19%), Query Frame = 0


Query: 57  ILSAFNDNARGS-TQINTNREMATKKLKPNDDVAHKLSSNAQLKSNNQIRGKAQVPIKRV 116
           I   F+ +A GS T +     + +  LKP+ D  H L   A + SN     +    +  +
Sbjct: 11  IFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLL--ASMDSNGNGFVEFDELVGTI 70

Query: 117 YDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDT 176
                  +  + EQ+ EIF+ +D D +GF++  E+  A A +G    + +    +   DT
Sbjct: 71  LPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADT 130

Query: 177 NKDGLIGEDELDKLI 191
           N DG+I   E   ++
Sbjct: 131 NGDGVISFGEFASIM 143

[Close]

BLAST of CSPI05G01500 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 41.6 bits (96), Expect = 8.8e-04
Identity = 18/62 (29.03%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 128 EQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELD 187
           E++K++F  +DS+ DG +++ E+   F  +G+ +   +    +   DT++DG I  DE  
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 188 KL 190
            L
Sbjct: 82  TL 83

[Close]

BLAST of CSPI05G01500 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 41.6 bits (96), Expect = 8.8e-04
Identity = 18/62 (29.03%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 128 EQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYTDTNKDGLIGEDELD 187
           E++K++F  +DS+ DG +++ E+   F  +G+ +   +    +   DT++DG I  DE  
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 188 KL 190
            L
Sbjct: 82  TL 83

The following BLAST results are available for this feature:

BLAST of CSPI05G01500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AWK2	4.6e-05	25.00	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LQN4	1.3e-04	24.84	Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 ...	[more]
Q6L5F4	2.3e-04	24.52	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8L3R2	8.6e-04	26.45	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]

BLAST of CSPI05G01500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLF3	6.2e-106	99.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0KIY4	3.7e-50	65.34	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0KMQ1	6.1e-45	59.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1	[more]
A0A6J1EP72	2.2e-42	57.80	calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1	[more]
A0A5A7VI66	1.4e-41	56.65	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of CSPI05G01500 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648234.1	5.1e-78	100.00	hypothetical protein Csa_018399 [Cucumis sativus]	[more]
XP_022930012.1	4.5e-42	57.80	calmodulin-2/4-like [Cucurbita moschata]	[more]
XP_023530739.1	7.6e-42	57.80	calmodulin-2/4-like [Cucurbita pepo subsp. pepo]	[more]
KAA0065465.1	2.9e-41	56.65	putative calcium-binding protein CML11 [Cucumis melo var. makuwa]	[more]
XP_022930019.1	1.4e-40	56.65	calcium-binding protein CML38-like [Cucurbita moschata]	[more]

BLAST of CSPI05G01500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32250.1	9.4e-06	24.84	Calcium-binding EF-hand family protein	[more]
AT3G50770.1	6.1e-05	26.45	calmodulin-like 41	[more]
AT1G18530.1	3.0e-04	25.93	EF hand calcium-binding protein family	[more]
AT1G18210.1	8.8e-04	29.03	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	8.8e-04	29.03	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 127..188 e-value: 2.5E-7 score: 31.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 125..160 score: 12.616518
None	No IPR available	GENE3D	1.10.238.10		coord: 28..195 e-value: 2.8E-11 score: 45.6
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 174..186
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 138..150
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 121..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G01500.1	CSPI05G01500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding