CSPI05G00740 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G00740
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase
Location	Chr5: 972255 .. 972671 (+)
RNA-Seq Expression	CSPI05G00740
Synteny	CSPI05G00740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCAACCCTATCATCCAAATGAAGCTGAAGAAGGACCCAGTGGAATCAAAGGACTTAGGAGACCAAAGTCCCAAGGGAAGGTATGCACGATGACGCTGATTAAGGCTAAGAACAGACTGCATGTGATTACTGGTTCGTTACTATTTTGTAACTCCCCTAATCTAGTGTTGTTTGATTTAGGAGCTATGCACTCTTTCATTTCTATATCACATGCAAAGTCTCTAGACCATGAGATAGAACCTTTAGAAGGTGGAATATTGGTTAGCACGACTTCAGATGAGGCGTTTATGGTAGAGTTTGTATGTAGGAATTGCGAGATAAGGATTAAAAATGTCACGTTGAAGGTGGACTTTATATTATTTGAGCTTCATGAGCTAGATGTGATCTTGGCAATGGATTTCCTTAACAAGTAA

mRNA sequence

ATGAACCAACCCTATCATCCAAATGAAGCTGAAGAAGGACCCAGTGGAATCAAAGGACTTAGGAGACCAAAGTCCCAAGGGAAGGTATGCACGATGACGCTGATTAAGGCTAAGAACAGACTGCATGTGATTACTGGTTCGTTACTATTTTGTAACTCCCCTAATCTAGTGTTGTTTGATTTAGGAGCTATGCACTCTTTCATTTCTATATCACATGCAAAGTCTCTAGACCATGAGATAGAACCTTTAGAAGGTGGAATATTGGTTAGCACGACTTCAGATGAGGCGTTTATGGTAGAGTTTGTATGTAGGAATTGCGAGATAAGGATTAAAAATGTCACGTTGAAGGTGGACTTTATATTATTTGAGCTTCATGAGCTAGATGTGATCTTGGCAATGGATTTCCTTAACAAGTAA

Coding sequence (CDS)

ATGAACCAACCCTATCATCCAAATGAAGCTGAAGAAGGACCCAGTGGAATCAAAGGACTTAGGAGACCAAAGTCCCAAGGGAAGGTATGCACGATGACGCTGATTAAGGCTAAGAACAGACTGCATGTGATTACTGGTTCGTTACTATTTTGTAACTCCCCTAATCTAGTGTTGTTTGATTTAGGAGCTATGCACTCTTTCATTTCTATATCACATGCAAAGTCTCTAGACCATGAGATAGAACCTTTAGAAGGTGGAATATTGGTTAGCACGACTTCAGATGAGGCGTTTATGGTAGAGTTTGTATGTAGGAATTGCGAGATAAGGATTAAAAATGTCACGTTGAAGGTGGACTTTATATTATTTGAGCTTCATGAGCTAGATGTGATCTTGGCAATGGATTTCCTTAACAAGTAA

Protein sequence

MNQPYHPNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHSFISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHELDVILAMDFLNK*

Homology

[Close]

BLAST of CSPI05G00740 vs. ExPASy TrEMBL
Match: A0A5A7UZA0 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold518G00110 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.1e-20
Identity = 62/137 (45.26%), Postives = 83/137 (60.58%), Query Frame = 0


Query: 3   QPYHPNEAEEGPSGI--KG-LRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLF 62
           QP H  +  EG SG   KG + RP+ QGKV  MT  +A++   VITG++L CN P  VLF
Sbjct: 258 QPGHFKKDSEGTSGARQKGVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLF 317

Query: 63  DLGAMHSFISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDF 122
           D GA HSF+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VD 
Sbjct: 318 DPGATHSFVSSIFLTKLNRMLEPLSEGLAIYTPVGDVLLVNEVLRNCEVLVEGISLLVDL 377

Query: 123 ILFELHELDVILAMDFL 137
           +  EL  LDVIL MDFL
Sbjct: 378 LPLELQRLDVILGMDFL 394

[Close]

BLAST of CSPI05G00740 vs. ExPASy TrEMBL
Match: A0A5D3CHM7 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold73G00100 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-19
Identity = 58/130 (44.62%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G    + +RRPK QGKV  MT  +A++   VITG++L CN P  VLFD GA HS
Sbjct: 325 PTEGTSGARQKRVVRRPKQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLFDPGATHS 384

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S +    L+  ++PL  G+ + T   +  +V  V RNCEI ++ ++L VD +  EL  
Sbjct: 385 FVSCTFMTKLNRMLKPLSEGLAIYTPVGDVLLVNEVLRNCEILVECISLLVDLLPLELQM 444

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 445 LDVILGMDFL 454

[Close]

BLAST of CSPI05G00740 vs. ExPASy TrEMBL
Match: A0A5A7U430 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold13G001700 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-19
Identity = 59/128 (46.09%), Postives = 82/128 (64.06%), Query Frame = 0


Query: 12  EGPSGI--KG-LRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHSFI 71
           EG SG   KG + RP+ QGKV  MT  +A++ L VITG++L CN P  +LFDLGA HSF+
Sbjct: 278 EGTSGARQKGVVGRPRQQGKVYAMTQQEAEDALDVITGTILICNVPADILFDLGATHSFV 337

Query: 72  SISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHELD 131
           S      L+  +EPL  G+ + T   +  +V  V RNCE+ +++++L VD +  EL  LD
Sbjct: 338 SSIFLTKLNRMLEPLSEGLAIYTPVGDILLVNEVLRNCEVLVEDISLLVDLLPLELQRLD 397

Query: 132 VILAMDFL 137
           VIL M+FL
Sbjct: 398 VILGMNFL 405

[Close]

BLAST of CSPI05G00740 vs. ExPASy TrEMBL
Match: A0A5A7VGR2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G004150 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-19
Identity = 56/130 (43.08%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G    + + RP+ QGKV  MT  +A++   VITG++L CN P  +LFD GA HS
Sbjct: 390 PTEGTNGARQKEVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADILFDPGATHS 449

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VDF+  EL  
Sbjct: 450 FVSSIFLTKLNRMLEPLSEGLAIYTPVGDVLLVNEVLRNCEVLVEGISLLVDFLPLELQR 509

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 510 LDVILGMDFL 519

[Close]

BLAST of CSPI05G00740 vs. ExPASy TrEMBL
Match: A0A5A7V0R0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold160G00330 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-19
Identity = 57/130 (43.85%), Postives = 77/130 (59.23%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G      + RP+ QGKV  MT  +A++   VITG++L CN P  VLFDLGA HS
Sbjct: 393 PTEGTSGARQKVVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLFDLGATHS 452

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VD +  EL  
Sbjct: 453 FVSSIFLTKLNRMLEPLSEGLAIYTLVGDVLLVNEVLRNCEVLVEGISLLVDLLPLELQR 512

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 513 LDVILGMDFL 522

[Close]

BLAST of CSPI05G00740 vs. NCBI nr
Match: KAA0059506.1 (uncharacterized protein E6C27_scaffold518G00110 [Cucumis melo var. makuwa])

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-19
Identity = 62/137 (45.26%), Postives = 83/137 (60.58%), Query Frame = 0


Query: 3   QPYHPNEAEEGPSGI--KG-LRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLF 62
           QP H  +  EG SG   KG + RP+ QGKV  MT  +A++   VITG++L CN P  VLF
Sbjct: 258 QPGHFKKDSEGTSGARQKGVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLF 317

Query: 63  DLGAMHSFISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDF 122
           D GA HSF+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VD 
Sbjct: 318 DPGATHSFVSSIFLTKLNRMLEPLSEGLAIYTPVGDVLLVNEVLRNCEVLVEGISLLVDL 377

Query: 123 ILFELHELDVILAMDFL 137
           +  EL  LDVIL MDFL
Sbjct: 378 LPLELQRLDVILGMDFL 394

[Close]

BLAST of CSPI05G00740 vs. NCBI nr
Match: TYK11035.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 106.3 bits (264), Expect = 2.2e-19
Identity = 58/130 (44.62%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G    + +RRPK QGKV  MT  +A++   VITG++L CN P  VLFD GA HS
Sbjct: 325 PTEGTSGARQKRVVRRPKQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLFDPGATHS 384

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S +    L+  ++PL  G+ + T   +  +V  V RNCEI ++ ++L VD +  EL  
Sbjct: 385 FVSCTFMTKLNRMLKPLSEGLAIYTPVGDVLLVNEVLRNCEILVECISLLVDLLPLELQM 444

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 445 LDVILGMDFL 454

[Close]

BLAST of CSPI05G00740 vs. NCBI nr
Match: KAA0049994.1 (uncharacterized protein E6C27_scaffold13G001700 [Cucumis melo var. makuwa])

HSP 1 Score: 106.3 bits (264), Expect = 2.2e-19
Identity = 59/128 (46.09%), Postives = 82/128 (64.06%), Query Frame = 0


Query: 12  EGPSGI--KG-LRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHSFI 71
           EG SG   KG + RP+ QGKV  MT  +A++ L VITG++L CN P  +LFDLGA HSF+
Sbjct: 278 EGTSGARQKGVVGRPRQQGKVYAMTQQEAEDALDVITGTILICNVPADILFDLGATHSFV 337

Query: 72  SISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHELD 131
           S      L+  +EPL  G+ + T   +  +V  V RNCE+ +++++L VD +  EL  LD
Sbjct: 338 SSIFLTKLNRMLEPLSEGLAIYTPVGDILLVNEVLRNCEVLVEDISLLVDLLPLELQRLD 397

Query: 132 VILAMDFL 137
           VIL M+FL
Sbjct: 398 VILGMNFL 405

[Close]

BLAST of CSPI05G00740 vs. NCBI nr
Match: KAA0066350.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 105.5 bits (262), Expect = 3.7e-19
Identity = 56/130 (43.08%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G    + + RP+ QGKV  MT  +A++   VITG++L CN P  +LFD GA HS
Sbjct: 390 PTEGTNGARQKEVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADILFDPGATHS 449

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VDF+  EL  
Sbjct: 450 FVSSIFLTKLNRMLEPLSEGLAIYTPVGDVLLVNEVLRNCEVLVEGISLLVDFLPLELQR 509

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 510 LDVILGMDFL 519

[Close]

BLAST of CSPI05G00740 vs. NCBI nr
Match: KAA0060066.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 105.1 bits (261), Expect = 4.8e-19
Identity = 57/130 (43.85%), Postives = 77/130 (59.23%), Query Frame = 0


Query: 7   PNEAEEGPSGIKGLRRPKSQGKVCTMTLIKAKNRLHVITGSLLFCNSPNLVLFDLGAMHS 66
           P E   G      + RP+ QGKV  MT  +A++   VITG++L CN P  VLFDLGA HS
Sbjct: 393 PTEGTSGARQKVVVGRPRQQGKVYAMTQQEAEDAPDVITGTILICNVPADVLFDLGATHS 452

Query: 67  FISISHAKSLDHEIEPLEGGILVSTTSDEAFMVEFVCRNCEIRIKNVTLKVDFILFELHE 126
           F+S      L+  +EPL  G+ + T   +  +V  V RNCE+ ++ ++L VD +  EL  
Sbjct: 453 FVSSIFLTKLNRMLEPLSEGLAIYTLVGDVLLVNEVLRNCEVLVEGISLLVDLLPLELQR 512

Query: 127 LDVILAMDFL 137
           LDVIL MDFL
Sbjct: 513 LDVILGMDFL 522

The following BLAST results are available for this feature:

BLAST of CSPI05G00740 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI05G00740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UZA0	6.1e-20	45.26	CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A5D3CHM7	1.0e-19	44.62	DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5A7U430	1.0e-19	46.09	CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A5A7VGR2	1.8e-19	43.08	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21...	[more]
A0A5A7V0R0	2.3e-19	43.85	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold16...	[more]

BLAST of CSPI05G00740 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0059506.1	1.3e-19	45.26	uncharacterized protein E6C27_scaffold518G00110 [Cucumis melo var. makuwa]	[more]
TYK11035.1	2.2e-19	44.62	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0049994.1	2.2e-19	46.09	uncharacterized protein E6C27_scaffold13G001700 [Cucumis melo var. makuwa]	[more]
KAA0066350.1	3.7e-19	43.08	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0060066.1	4.8e-19	43.85	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]

BLAST of CSPI05G00740 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF08284	RVP_2	coord: 26..138 e-value: 8.5E-27 score: 93.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	CDD	cd00303	retropepsin_like	coord: 47..137 e-value: 2.74164E-10 score: 51.5684
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 24..138 e-value: 4.8E-11 score: 44.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G00740.1	CSPI05G00740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
molecular_function	GO:0016787	hydrolase activity