CSPI04G26700 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G26700
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptiontRNA pseudouridine(55) synthase
LocationChr4: 23716841 .. 23723288 (-)
RNA-Seq ExpressionCSPI04G26700
SyntenyCSPI04G26700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCCCTTCCCTTGCCGCATATAAACTGAAAGCGCACTATACTAACAATTCGGCGGACAACTCTCTCTCTCTCTGCACAGAGTTGCTTCCCGCAGTTCTTCAATGGCCAACGAACAGGACGAAGTTCAAGCTAATCCTGAGGCTACAGCACTCGCACTTACTTCCGTCACTCAAGAGGCCATTTCTCCCGGTGACAAACTGCAGATCCCTTCTTACGCCACAAAAGACCTGTTGTCGATTGGCGTATGTTTTCTTGTCTTTAAACCTTCGATATCGTAGTACTAAATACTCGTGGTTACGAATGCGATCAATTTCTCTCACTTCATATTGTCTTAGTTTGTAGTTTCAAGCTATTTCTAGTCGTGTTTGAATGTGCTTGTTCATTTTGTCGTTATCCTTGATTTTTTTTTTGTTCACTTCAAACTAAAACCTCCAGAGTTGAGAATCGCCGTCGTTAGAAATGCTATCGACGAAGCTATCACTTCCTAACTTGATGATTTTCAGTTTGGAGGAAGTTTCTGGTTGTGTCTGAACTTTGAATGTGTTTCTATATTCAGTCGCTATTCTTAATTTTTCTTCGCAACCTAAATTTTCGTAGGCAATTATAGGAATGCAATCGATTTAACTTTCGCTTCTTAAATTGATAGTTTATAATTAGGAGGAAACTCTTGAATGTGCTTTTTCATTCGATCGCTAACCTCAAATTTTCCTCACTTGAACCTAGGATTTCATAGCGTAATAACCGTCGATAGGAAGGCGATTAATATAACGAACATCACTTTTATCTTATATCTTGATAGCCTGTAGTTTGGAGGAAATTGCTTTTGTGTTGAATGTGTTCCTTCATTCGATCATTATTCTTGATTTTTCTACGTCACCGAGGTAGCGCTATTTATTATTGTGACACCTTTAACATGATAAGTCATCTTTTATGTTTCCAAATTTCAGTTCTACGTGTTCCGTTTATTTTCTATTTATACAGTAAACACGAGTTAGGGCTGTTAACTTTCGCTTAATAAGTTGATTTTCTAGCTTTTCAAGACGTCCTGTTTTATATATACTCTCGCTTGATTTTTAGCAATCTGCAGTTTTACAAGTCCTATTAAATTCTTGAAAATAATTATGTCTGTTATTACTGGCGACTGGCGTAATGCTAATGATATTGGTTTTATTATAACAATAAAAAATCAGAAGATGTTTATGGAATCATGATGGAAGTAGCATCGGCAGCAGCATTATTTTTTCCTTTTTGCTCTTTTTTTATAATTTAATTTTATCGGTGCCTAATAGTGGTCTACAGCTGAGCTGATCAGCATCTATTATGTTGTAGGATACATTGGGCGGTGTTTGCCTGCTTTCATATTGGCATGCATTTACTATTTCAAGCAATAGTATTGGCATGTATTGGAATTGCATCGATGGTACTGTTTAGGATAGCCAGAAGATGTTTGATCGTTACTTTGCTTGCAGGTATGCATCCCCTGCATCATTCGCCTATCTGGCATTGAAAGGCACAGTTATTCCTTTTCATTGTCAGCACCAATCCTAGGTTCTGATAACGAGGCAATTGATGATGATAATGATAGGAACACTTGCTGTCTATGTTTAGGTATTTTACAGTTCAGTTACATTGATGATAAAGGTGACTTTATAAAATGTAAAGGAGTTGATGATTTAGCTGTCTCGATTTCTGAGATGGTAAAGAAAGAGGGCCATCAGATTGATAGCTTTTCTCTTGAGGCGTCAGTTCCTTCAATCATTCTGGAGAATGAGAAGCGTGTTTGGTAAGAGCAACCCCCCCCCCCCCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTCCCCCCCCAAAATAAATAAATAAATAAATAGATAAAGGGAGACAGTTCTGTTGGTTAGGTTAAATGTGTGTGTGTTTCCAATAACAATATAAATTGCTTTGATTTTCATTTTCGTTTATGGTTTTTGTATTCTGAACAGAACTGTAGTTAACTTTTCATGTAAGAAGAAAGCTTTGTGTTAATACTAATACATTAGGAGAGTGCCATGAGTGAATGTGCGTACAAAGAGACTAAGCCGAGATGTTGGAAAAGAGATTGCAGAAGGAAATTACTGAGGGAAAGTAAGCGCAAAGATTAATGAAATAAATTGCCTATAGAAAGTGAAAAACAAGTTCATAACTAGAGCATATTATCTAACAAATGATTTGAAAAATAAAAAATTGGATACATATTGTTTTTCGCTCTTACATGGGCTGATCTATTGTAAAATTCTTTTGAAGACAAAGTGAAGTGTTAGATTAGCAGAAAAAAATGATTATCATATAAGCTCTAAAACGATAACCTCTTTGAAAGTTATTGTGATTGGGAGATTTCATATGTGACATGGCTGAAGTGGTTTATCCTCTTAAAACCTCTTTCGTTTGTGCTGCCAAACCTTGCTGACGTTGGATTTTACTTAGTCCATTTAATGTGCCAGTTCATATTGTAGTTACTATTTTTCATTTCTGCATTGCAGGCTGTATTTGAAAAGAAAATATGGGTCTGAACAGTGGTTTCAAGGCAAGCCTCCTCCTATATGTCTTTCAGCGAAGGATGCCTTAAAAATCTGTATAACAAAGCCTCTTGAAACTTTACTGGTGAGTGCATTCTCCATTGTTTACCGAAGATAAGTGTTTATCTTATGCAAAATGATGCCAGCGAGCCTTTCACAATGAAGAGGACTATTTTTTAGTTTTTCTTTAAAAAAAAATTATTATTTGGGTATTTGAAAGGAAAAAGTTCATTGATTAATATTTATCCATGAAGTTATGCAGGATTGCAAGTCTAGTTCAAGTGGCTTGCGCATCCGGTTAACTTATAGTCATCCTAAAGCTTCAAATAGCAATGGTAATTCCATCAAAAGAAACCAGGATGGCTTCAAGATGAGAAAAATCGGTTAGTTAGTTGAACCTTTGCTATTAATTGTCACTACTCTTCTGTATAGATTCAGAAACAAGAAGTTATCTATCGCTTTACCTTCAAATTTTCTCATTCTCATCAACTTTATTAGATGTCAAGCCCTAGTAATCTATATGCTTGGAAGTACATGCATGGTAAATTCATACCAAAACCTATTGCAAATTCGCAATACTATATACCTTAATTAGAAAGTGCAAGAGATTTGAGAAACATAGAGTTGTAGTTCATTTTCTTAAATCACACACATAGATGCCGAAAAAGTCATTTGAAACGTGTCCATATTGTCATTGCACTCCTTTGTGAAGAACTCTGCTGTCCGATTATCACCATCTTGAAATTTATTGGAGTCTAAATATTTGTAGCTGTGTACAAATTTCTTTGATGGGCCTTTCAAGCCAAATTCTGTTTGTAGCAGAAATTGTATCCCAAGAAAGATCCGTAGTTAAGGATATAAGATTTATTATTATTGTCATTATTGTTATTATTATTTTTGGATGAAGTTAGTACTACTTTCCTTTTTTCCATTAGCCATTAGCCATTAGCCATTTGGAGTTGGAGTTGTTCTCCAAAGTAACTATTATATTTAAAAATGTCTTATCTGTAGCAGAAAGTTGTACAGGAGAGCCCAATTCTATTTCTTCTCTTAGAGGTGATTGTTTTACTTCCTAAACCCAATCAAGATGTTGCAGCGAGTCCCTTTACCTTTAGGAACCTAGAGTAGGGAATTGATTTGTATTTCTTTACATGTATTTAATCAGTTACCTTCAACATGTAGTTTCCGGAAAGGAAAAAAAAAAACAAAGTTACCTTTGACATGTGAACTGAGATGGCTGTTGAAGCCAATGATTGTAGCTCAGAGACTATTGATGATTCTATTGCAGTTGGCAGTGATGAACCCTCGGATATTTCAAACTGTAATGCCGTTGCTGATGATTGTATCAACCCTTCACAAGAAGTTGTTTTTCCTCAAGAAACGGTAAGTTTGTTCTATACTATTTAAATGATTAAAAAGATGATGTCACTGACTAAATGAACTTCGGTCCTTATGTCCTAGATATTAAAAAAATGGTGACCTTGACCAATTGATTTCAATTTCATCATTGGAAGTGTAATATTGGAAAAAAATGAATGGAGGTATAAGATTTTGATTAAAGGATTTAAAGTCAATAAACTGCAGAGTTCATGATGTAGAAAATGATGGTGTATCTTTTTCTTTTTATCGTTTTCTATTCTCGGTTTAAGGGACTCAAGATCAACTCTTTGTTGTGCATTGTAATCTGATCCTTCGCATTCAGATTTTTGTTACATATTTTATTGGTTACTAATGGTGGTACTCTCTGTTTCTCGTCAATTCTACGTAGATCAAAGAACCATGCCATTTGGCTTTAACTTGTTATAGAACTTCGATATACATAGTTGGCAGATATATAAAGGTAAATAAGTTTTATTTTCTACATTTCATTTTTCCCCAAAGACTTGGTTAAGTACTTCACTGATTCATAACCTCCTGGATTATTAACTTTTTTATTGTAGTATTCAAGAAATGTGAGTCAGACACGTTGGGTAATTGGTGAAGAGAGAATGGGAGAAGCATCTGTCGAGGTAGTGATTTTTGACTTTGGTGACTGGCTGTTCTATTATACAACTGTTCATTTAGCAAGAAAAAGTTTACAGTACCATGGGTATCTGCAGGAGATTTTAGGCAACAATATTCTTCCCTTCTGTCGTGGTGATAGTTACAAGTTTCATGCTGCTGGTAGAGAGGATCTCGATGTAATATTCTTCCCTTATTGACAATCATGATTCTCTCTACTGTTTGTAATATTAAATTTTCTTGTCAGGTTCGAATGTTGGGTTCAGGTAGACCTTTCTTGGTTGAGATACAGAACGCACGTCTTCTCCCATCTGAGATGATTATAAATGAAATACAGTCGAAGATAAATAGTTCAGAAAATAAATTGGTAACACACTGTTCATATTGTACTGCTTCTTCCATTGATTAAAATTACTGCAAACTTCATATGAATCACATTACTATTTCAGATTGGCGTAAGAAATCTGAAAGTCGTAGGCAGCGAGGGTTGGGCCCTGGTTCAAGAAGGAGAAGCAGAGAAGCAGGTTTTCTCTATGATCAATCTTTTTTTCAATTACAAATACAAAATTCTGTTTAATTCTGTATTTGTGACTCTTTAAATCAATGCAGAAACAATATGCTGCACTTGTGTGGACATCTCGCCCACTCGAGGATGCTGATCTTTTATCAATATCATCACTCAAAGACTTGGTGAGGAGTTTAGCAAGCTTGATGCTTTGATTTCATATCTCATATGATTTAAGCGTTACATTTTAGAACTATGCTTATGAAATTATTTTTCTTTTGTGCAGAAAATTTTGCAAAGAACTCCAATAAGAGTGCTTCATCGCCGAAGTCCATTAGAACGTGAAAAGATCATCCACTGGTTAGAGTCCCTTTTCCAATCGTACAGTAATGCTACAATATCCACTCATGCTTCCTCTGAGGGTCACTGACGATGTTCTCTTTTTTCATTTTAGGATGAAGATTGAGAAGGTTGCTGAAAGTTCTCAGTATTATCTCCTACATTTGTGTACCCAGGTATCTATATACATTATACATAAACCCCTAAATTACCTTGACCTTTCTATTGAAATTGTTTAAAATCGTAATCTAAAAGATGGTGGTAAACACCAAAATTCGCAAACCTTGTTAAAGAAAAAGTGAAAAGCATAAATGATTAGTTGAATCGGATGTAAACCCTAAACTTTTAAATAGTAAAATGTAAAAGACTAACTATTACTGTATGTGTTGCATAGGCTGGAACATACATTAAAGAATTCGTCCATGGGGATCTTGGTCGGACTCATCCCAGGTATGAGGAACTCTTAATCAGGTTGTTTGGTAGTAACGACATTGTTGAGTTGTAATTTGTCTTCACATCTATTTAACGGATTTGTTATCGGTTACAGTATAGGATCCATTCTTAGTTGCCGAGCCGAGATTCTGCAACTTGACGTTACGGATATAAAAATGGACTGTCATTTGGGTGAATAAAAGAAAGGTTAGATCAAGGTGTGTATTCCTGCTCTTCAACTCTGGCTCTCATTCTCGTGGATTTTGCAAAAATTGTATTCCTCTTTTCATCATGTTGGACCCCAACTTATGACGTTATCAACAGGTTCATTGATAGAAGAAGGAGAAGGATGATGATTCCATATTAGTTAGTTTTCGTTTCATCACTCCACTTTGGTGCCCTTCTACTCCTCCCTCAATTTGGTAATTTCAAATAGATTTATTTGATTTGGTAATTTTAAAATTTGATTAACCTAAACTTTTATTTTCCCCGTGATTCATTGTTGGAGGCGTATTGAATTTACAAGTTATAAAGGGTAAGGTCAAAACGTACTTTCAAAATTAAGCACTGTGGCATTGTATTGTCAGAGTGAGGTTA

mRNA sequence

CCTTCCCTTCCCTTGCCGCATATAAACTGAAAGCGCACTATACTAACAATTCGGCGGACAACTCTCTCTCTCTCTGCACAGAGTTGCTTCCCGCAGTTCTTCAATGGCCAACGAACAGGACGAAGTTCAAGCTAATCCTGAGGCTACAGCACTCGCACTTACTTCCGTCACTCAAGAGGCCATTTCTCCCGGTGACAAACTGCAGATCCCTTCTTACGCCACAAAAGACCTGTTGTCGATTGGCGTATGCATCCCCTGCATCATTCGCCTATCTGGCATTGAAAGGCACAGTTATTCCTTTTCATTGTCAGCACCAATCCTAGGTTCTGATAACGAGGCAATTGATGATGATAATGATAGGAACACTTGCTGTCTATGTTTAGGTATTTTACAGTTCAGTTACATTGATGATAAAGGTGACTTTATAAAATGTAAAGGAGTTGATGATTTAGCTGTCTCGATTTCTGAGATGGTAAAGAAAGAGGGCCATCAGATTGATAGCTTTTCTCTTGAGGCGTCAGTTCCTTCAATCATTCTGGAGAATGAGAAGCGTGTTTGGCTGTATTTGAAAAGAAAATATGGGTCTGAACAGTGGTTTCAAGGCAAGCCTCCTCCTATATGTCTTTCAGCGAAGGATGCCTTAAAAATCTGTATAACAAAGCCTCTTGAAACTTTACTGGATTGCAAGTCTAGTTCAAGTGGCTTGCGCATCCGGTTAACTTATAGTCATCCTAAAGCTTCAAATAGCAATGGTAATTCCATCAAAAGAAACCAGGATGGCTTCAAGATGAGAAAAATCGTTGGCAGTGATGAACCCTCGGATATTTCAAACTGTAATGCCGTTGCTGATGATTGTATCAACCCTTCACAAGAAGTTGTTTTTCCTCAAGAAACGATCAAAGAACCATGCCATTTGGCTTTAACTTGTTATAGAACTTCGATATACATAGTTGGCAGATATATAAAGTATTCAAGAAATGTGAGTCAGACACGTTGGGTAATTGGTGAAGAGAGAATGGGAGAAGCATCTGTCGAGGAGATTTTAGGCAACAATATTCTTCCCTTCTGTCGTGGTGATAGTTACAAGTTTCATGCTGCTGGTAGAGAGGATCTCGATGTTCGAATGTTGGGTTCAGGTAGACCTTTCTTGGTTGAGATACAGAACGCACGTCTTCTCCCATCTGAGATGATTATAAATGAAATACAGTCGAAGATAAATAGTTCAGAAAATAAATTGATTGGCGTAAGAAATCTGAAAGTCGTAGGCAGCGAGGGTTGGGCCCTGGTTCAAGAAGGAGAAGCAGAGAAGCAGAAACAATATGCTGCACTTGTGTGGACATCTCGCCCACTCGAGGATGCTGATCTTTTATCAATATCATCACTCAAAGACTTGAAAATTTTGCAAAGAACTCCAATAAGAGTGCTTCATCGCCGAAGTCCATTAGAACGTGAAAAGATCATCCACTGGATGAAGATTGAGAAGGTTGCTGAAAGTTCTCAGTATTATCTCCTACATTTGTGTACCCAGGCTGGAACATACATTAAAGAATTCGTCCATGGGGATCTTGGTCGGACTCATCCCAGTATAGGATCCATTCTTAGTTGCCGAGCCGAGATTCTGCAACTTGACGTTACGGATATAAAAATGGACTGTCATTTGGGTGAATAAAAGAAAGGTTAGATCAAGGTTCATTGATAGAAGAAGGAGAAGGATGATGATTCCATATTAGTTAGTTTTCGTTTCATCACTCCACTTTGGTGCCCTTCTACTCCTCCCTCAATTTGGTAATTTCAAATAGATTTATTTGATTTGGTAATTTTAAAATTTGATTAACCTAAACTTTTATTTTCCCCGTGATTCATTGTTGGAGGCGTATTGAATTTACAAGTTATAAAGGGTAAGGTCAAAACGTACTTTCAAAATTAAGCACTGTGGCATTGTATTGTCAGAGTGAGGTTA

Coding sequence (CDS)

ATGGCCAACGAACAGGACGAAGTTCAAGCTAATCCTGAGGCTACAGCACTCGCACTTACTTCCGTCACTCAAGAGGCCATTTCTCCCGGTGACAAACTGCAGATCCCTTCTTACGCCACAAAAGACCTGTTGTCGATTGGCGTATGCATCCCCTGCATCATTCGCCTATCTGGCATTGAAAGGCACAGTTATTCCTTTTCATTGTCAGCACCAATCCTAGGTTCTGATAACGAGGCAATTGATGATGATAATGATAGGAACACTTGCTGTCTATGTTTAGGTATTTTACAGTTCAGTTACATTGATGATAAAGGTGACTTTATAAAATGTAAAGGAGTTGATGATTTAGCTGTCTCGATTTCTGAGATGGTAAAGAAAGAGGGCCATCAGATTGATAGCTTTTCTCTTGAGGCGTCAGTTCCTTCAATCATTCTGGAGAATGAGAAGCGTGTTTGGCTGTATTTGAAAAGAAAATATGGGTCTGAACAGTGGTTTCAAGGCAAGCCTCCTCCTATATGTCTTTCAGCGAAGGATGCCTTAAAAATCTGTATAACAAAGCCTCTTGAAACTTTACTGGATTGCAAGTCTAGTTCAAGTGGCTTGCGCATCCGGTTAACTTATAGTCATCCTAAAGCTTCAAATAGCAATGGTAATTCCATCAAAAGAAACCAGGATGGCTTCAAGATGAGAAAAATCGTTGGCAGTGATGAACCCTCGGATATTTCAAACTGTAATGCCGTTGCTGATGATTGTATCAACCCTTCACAAGAAGTTGTTTTTCCTCAAGAAACGATCAAAGAACCATGCCATTTGGCTTTAACTTGTTATAGAACTTCGATATACATAGTTGGCAGATATATAAAGTATTCAAGAAATGTGAGTCAGACACGTTGGGTAATTGGTGAAGAGAGAATGGGAGAAGCATCTGTCGAGGAGATTTTAGGCAACAATATTCTTCCCTTCTGTCGTGGTGATAGTTACAAGTTTCATGCTGCTGGTAGAGAGGATCTCGATGTTCGAATGTTGGGTTCAGGTAGACCTTTCTTGGTTGAGATACAGAACGCACGTCTTCTCCCATCTGAGATGATTATAAATGAAATACAGTCGAAGATAAATAGTTCAGAAAATAAATTGATTGGCGTAAGAAATCTGAAAGTCGTAGGCAGCGAGGGTTGGGCCCTGGTTCAAGAAGGAGAAGCAGAGAAGCAGAAACAATATGCTGCACTTGTGTGGACATCTCGCCCACTCGAGGATGCTGATCTTTTATCAATATCATCACTCAAAGACTTGAAAATTTTGCAAAGAACTCCAATAAGAGTGCTTCATCGCCGAAGTCCATTAGAACGTGAAAAGATCATCCACTGGATGAAGATTGAGAAGGTTGCTGAAAGTTCTCAGTATTATCTCCTACATTTGTGTACCCAGGCTGGAACATACATTAAAGAATTCGTCCATGGGGATCTTGGTCGGACTCATCCCAGTATAGGATCCATTCTTAGTTGCCGAGCCGAGATTCTGCAACTTGACGTTACGGATATAAAAATGGACTGTCATTTGGGTGAATAA

Protein sequence

MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIERHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSDISNCNAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE*
Homology
[Close]
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match: Q3MIT2 (tRNA pseudouridine synthase Pus10 OS=Homo sapiens OX=9606 GN=PUS10 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.2e-66
Identity = 163/440 (37.05%), Postives = 252/440 (57.27%), Query Frame = 0 

Query: 83  DNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEASVPS 142
           +++ N C +CLGILQ      + DFIK          + + V+  G +  S     S P 
Sbjct: 103 NSNLNVCNVCLGILQEFC---EKDFIK---------KVCQKVEASGFEFTSLVFSVSFPP 162

Query: 143 IILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPL--ETLLDCKSSSSG 202
            +   E   WL +K++ G +    G+   + L  K+A K  IT PL  E L       S 
Sbjct: 163 QLSVREHAAWLLVKQEMGKQSLSLGRDDIVQL--KEAYK-WITHPLFSEELGVPIDGKSL 222

Query: 203 LRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGS--DEPSDISNCNAVADDCINPSQEV 262
             + + ++HP+             D FK  K   S     + +   N + ++  +  ++ 
Sbjct: 223 FEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEE--DFLKQF 282

Query: 263 VFPQETIKEPCH-LALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNN 322
             P  + K  C  L + C   ++++ GRY KYSRN+ QT W+I  ER  E+SVEE++ ++
Sbjct: 283 PCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDH 342

Query: 323 ILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNA-RLLPSEMIINEIQSKINSSEN 382
           +L   + +S+ F ++GRED+DVR LG+GRPF +E+ N  R+  +   I E+Q KIN+S N
Sbjct: 343 LLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSN 402

Query: 383 KLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDADLLSISSLKDLKILQRT 442
           K I VR+L++V  E    ++EGE EK K Y+AL+WT++ ++  D+  ++ +KDLKI Q+T
Sbjct: 403 K-IQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKT 462

Query: 443 PIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIG 502
           P+RVLHRR    R ++IH+M+ + V E   ++ LHL TQAGTYIKEFVHGD GRT P+IG
Sbjct: 463 PLRVLHRRPLAVRARVIHFMETQYVDE--HHFRLHLKTQAGTYIKEFVHGDFGRTKPNIG 522

Query: 503 SILSCRAEILQLDVTDIKMD 517
           S+++  A+IL+LDV  + +D
Sbjct: 523 SLMNVTADILELDVESVDVD 522

[Close]
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match: Q9D3U0 (tRNA pseudouridine synthase Pus10 OS=Mus musculus OX=10090 GN=Pus10 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.9e-63
Identity = 164/450 (36.44%), Postives = 243/450 (54.00%), Query Frame = 0 

Query: 76  DNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFS 135
           D    +  +    C +CLGILQ     +KG   K          + + V+  G +  S  
Sbjct: 94  DESMAEKPSKLRVCNVCLGILQ--EFCEKGFITK----------VCQKVEASGFEFTSVV 153

Query: 136 LEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPL--ETLLD 195
           L  S P  +   E   WL +K++ G +    G+   + L  K+A K  IT PL  E L  
Sbjct: 154 LSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRNDVVQL--KEAYK-WITHPLFSEELGV 213

Query: 196 CKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSDISNCNAVADDCIN 255
                S   + + ++HP+ +       +  +D FK  K         +    AV      
Sbjct: 214 PTDGKSLFEVSVVFAHPETAEDCHFLGEVCRDCFKPAK-----NKQSVFTRMAVLKALSK 273

Query: 256 PSQEVVF-----PQETIKEPCH-LALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMGE 315
             +E        P  + K  C  L + C   ++++ GRY KYSRN+ QT W+I  ER  E
Sbjct: 274 IKEEDFLGQFPCPPNSPKTVCTVLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKME 333

Query: 316 ASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNA-RLLPSEMIINE 375
           +SVEE++ +++L   R +S+ F ++GRED+DVR LG+GRPF VE+ N  R+  +   + E
Sbjct: 334 SSVEELISDHLLAVFRAESFNFSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQEMKE 393

Query: 376 IQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDADLLSISS 435
           +Q  IN S +K I VR+L++V  E    ++EGE EK K Y+AL+WT+R ++  D+  +  
Sbjct: 394 LQQTINKSSDK-IQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGFLDD 453

Query: 436 LKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHG 495
           LKDLKI Q+TP+RVLHRR    R + IH MK   + E   ++ LHL TQAGTYIKEFVHG
Sbjct: 454 LKDLKIDQKTPLRVLHRRPLAVRTRAIHSMKTHYLDE--HHFRLHLKTQAGTYIKEFVHG 513

Query: 496 DLGRTHPSIGSILSCRAEILQLDVTDIKMD 517
           D GRT P++GS+++  A+IL+LDV  + +D
Sbjct: 514 DFGRTKPNLGSLMNVTADILELDVESVDVD 520

[Close]
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match: Q6ING2 (tRNA pseudouridine synthase Pus10 OS=Xenopus laevis OX=8355 GN=pus10 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.7e-62
Identity = 158/453 (34.88%), Postives = 244/453 (53.86%), Query Frame = 0 

Query: 75  SDNEAIDDDNDRN------TCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSISEMVKKEG 134
           S NE + +  D N       C LCLGILQ         F + + ++ + V I+  V    
Sbjct: 76  SSNEHLGNCEDVNGSQVVRICPLCLGILQ--------QFCEPEFIEKVFVKINSAV---- 135

Query: 135 HQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPL 194
           +++  F L  S+P+ +   E   WL  K++ G       K   + L  K+A K  I   L
Sbjct: 136 YELKDFVLSISLPAQLSVREHSAWLQAKQEMGKHGHSLDKTDIVQL--KEAYKWIIHPLL 195

Query: 195 ETLLDCKSSSSGL-RIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGS--DEPSDISNCN 254
              L  ++ S  L  + + ++HP+            +D FK  K   S     + +    
Sbjct: 196 SDQLGIQADSKSLFEVAVVFTHPETDGDCHFLATVCRDCFKPTKNKQSVFTRMAVVKALE 255

Query: 255 AVADDCINPSQEVVFPQETIKEPCHLA-LTCYRTSIYIVGRYIKYSRNVSQTRWVIGEER 314
            + ++      +  FP  + +  C +  + C  + +++ GRY KYSRN+ QT W+I  ER
Sbjct: 256 KIKEEDFR--VQFPFPPSSPETTCEVVDIQCNHSPVFVAGRYNKYSRNLPQTPWIIDGER 315

Query: 315 MGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLP-SEMI 374
             E+SVEE++ ++++   R  S+ F ++GRED+DVR LG GRPF +E+ N   +  +   
Sbjct: 316 KIESSVEELITDHLVAAFRSSSFNFSSSGREDVDVRTLGKGRPFAIELLNPHKVQFTGQE 375

Query: 375 INEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDADLLS 434
           I  +Q KIN+S+   I VR+L++V  E  A ++EGE EK K Y AL+W  + + + DL  
Sbjct: 376 IKALQQKINTSDK--IKVRDLQIVSREAVAHMKEGEEEKTKCYCALIWIEKMVRNEDLQL 435

Query: 435 ISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEF 494
           +  L++L I Q+TP+RVLHRR    R + IH M+ E V E   ++ L+L TQAGTYIKEF
Sbjct: 436 LDGLEELTIAQKTPLRVLHRRPLASRSRTIHTMRTEYVDE--HHFRLYLKTQAGTYIKEF 495

Query: 495 VHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD 517
           VHGD GRT P++GSI+   A+IL+LDV  + +D
Sbjct: 496 VHGDFGRTKPNVGSIMETNADILELDVESVDVD 508

[Close]
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match: Q9VPK7 (Putative tRNA pseudouridine synthase Pus10 OS=Drosophila melanogaster OX=7227 GN=Pus10 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 4.5e-36
Identity = 117/395 (29.62%), Postives = 195/395 (49.37%), Query Frame = 0 

Query: 136 LEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCK 195
           L   +P  +   +  +W  L+R++G     +  PP + +  K+A+K+ +   +   L   
Sbjct: 105 LAICLPMSLQLRQLAMWFALRRRFG-PSIDENNPPDVPI--KEAVKLILHPIICEKLAKV 164

Query: 196 SSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSDISNCNAVADDCINPS 255
             ++GL I +  +H          +K N++ F  +    +     I     + +    PS
Sbjct: 165 YDANGLMINIDLTHSLEGEEVEKLVKLNKEAFPAK----ASHQKRIEISRGLLEKQYQPS 224

Query: 256 Q------EVVF--PQETIKEPCHL-ALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMG 315
           +      E  F  P   ++E   L ++      I + GRY K SR +S T W++  +R+ 
Sbjct: 225 KVKAETYEKYFQIPCSNVEESIKLTSIDLQGPLICVAGRYRKLSRELSHTPWILNGQRLM 284

Query: 316 EASVEEILGNNILPFCRGDSYK--FHAAGREDLDVRMLGSGRPFLVEIQNAR---LLPSE 375
           E S+EEI+  ++ P       K  F ++GRED+DVR LG GRPF++EI NA+   L   E
Sbjct: 285 EDSIEEIIIRHVGPHFTEKLGKITFMSSGREDVDVRCLGKGRPFVLEIPNAKRSCLTKDE 344

Query: 376 MIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDADL 435
                ++  +++S   ++ + +L+VV  E    ++ GE +K+K Y AL     P+    L
Sbjct: 345 AF--AMERAVDAS--GMVSIHHLQVVPREELTHIKTGEEQKKKFYRALCALQEPVSVKIL 404

Query: 436 LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIK 495
             +       I Q+TPIRVLHRR    R + I+ +  +    + +  ++ + TQAGTYIK
Sbjct: 405 EQLQISASFNIQQKTPIRVLHRRPLHTRPRTIYSVTAKVHRGNPKALIIDIVTQAGTYIK 464

Query: 496 EFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD 517
           E VHG+ GRT PS+ SI+    +I  LDV  I +D
Sbjct: 465 ELVHGEFGRTTPSLSSIIGKPMDIQALDVVGIDLD 488

[Close]
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match: A1RX33 (tRNA pseudouridine synthase Pus10 OS=Thermofilum pendens (strain DSM 2475 / Hrk 5) OX=368408 GN=pus10 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.7e-35
Identity = 90/245 (36.73%), Postives = 136/245 (55.51%), Query Frame = 0 

Query: 272 ALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMG-EASVEEILGNNILPFCRGDSYKFH 331
           A+  +   +++ GRY KY+R + Q  W   +ER+    S+EE++    L     +  KFH
Sbjct: 200 AIELHPAPVFVYGRYRKYARGLPQNPWPQPDERIKFNTSIEELIVKPALELFEAEKAKFH 259

Query: 332 AAGREDLDVRMLGSGRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSE 391
           AAGRED+DVR LG+GRPF++EI+  R     + +  +  KINS    LI V +L     +
Sbjct: 260 AAGREDIDVRTLGTGRPFVLEIKKPR--KRNIDLKVLAEKINSGAGGLIEVLDLAYTDRK 319

Query: 392 GWALVQEGEAEKQKQYAALVWTSRPLEDADLLSISSL-KDLKILQRTPIRVLHRRSPLER 451
               ++   +  +K Y A V   +P++D  L  IS +  +  I QRTP RVLHRR    R
Sbjct: 320 TIKKLKSLASIAKKAYVARVKFEKPVDDEKLAEISKVFSNAVINQRTPTRVLHRRVDKLR 379

Query: 452 EKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLD 511
           +KI++ ++  K+++    + L   TQ G Y+KEF+HGD GRT PSI   L      ++LD
Sbjct: 380 KKIVYRLEARKISQDEVEFYLE--TQGGFYVKEFIHGDNGRTTPSIAEFLGNNVLSIELD 439

Query: 512 VTDIK 515
           V  I+
Sbjct: 440 VVSIE 440

[Close]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match: A0A0A0L378 (tRNA pseudouridine(55) synthase OS=Cucumis sativus OX=3659 GN=Csa_4G653400 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.3e-300
Identity = 518/521 (99.42%), Postives = 519/521 (99.62%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RHSYSFSLSAPILGSDNEAIDD NDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHSYSFSLSAPILGSDNEAIDDANDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240

Query: 241 ISNCNAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI 300
           ISNCN+VADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI
Sbjct: 241 ISNCNSVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI 300

Query: 301 GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS 360
           GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS
Sbjct: 301 GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS 360

Query: 361 EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD 420
           EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD
Sbjct: 361 EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD 420

Query: 421 LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI 480
           LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI
Sbjct: 421 LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI 480

Query: 481 KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE
Sbjct: 481 KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 521

[Close]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match: A0A1S3BUF4 (tRNA pseudouridine(55) synthase OS=Cucumis melo OX=3656 GN=LOC103493791 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 4.7e-291
Identity = 501/523 (95.79%), Postives = 509/523 (97.32%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240

Query: 241 ISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
           ISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300

Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
           VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360

Query: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
           PSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420

Query: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
            D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVA+SSQYYLLHLCTQAGT
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKSSQYYLLHLCTQAGT 480

Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 523

[Close]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match: A0A5D3D906 (tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00830 PE=3 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 2.9e-288
Identity = 502/537 (93.48%), Postives = 509/537 (94.79%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKI-------- 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI        
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKISNDCSLET 240

Query: 241 ------VGSDEPSDISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVG 300
                 VGSDEPSDISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ G
Sbjct: 241 IDDSIAVGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300

Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
           RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS
Sbjct: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360

Query: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQK 420
           GRPFLVEIQNARLLPSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420

Query: 421 QYAALVWTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAES 480
           QYAALVWTSRPLED D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVA+S
Sbjct: 421 QYAALVWTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKS 480

Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 537

[Close]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match: A0A5A7V921 (tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003460 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.7e-286
Identity = 501/553 (90.60%), Postives = 509/553 (92.04%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240

Query: 241 ISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
           ISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300

Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
           VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360

Query: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
           PSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420

Query: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHW----------------------- 480
            D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHW                       
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWLESFFNCTVKLQSTHASTKVTDN 480

Query: 481 -------MKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 522
                  MKIEKVA+SSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL
Sbjct: 481 VLLFHYRMKIEKVAKSSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 540

[Close]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match: A0A6J1IEB1 (tRNA pseudouridine(55) synthase OS=Cucurbita maxima OX=3661 GN=LOC111476467 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.3e-260
Identity = 461/537 (85.85%), Postives = 484/537 (90.13%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKL--------QIPSYATKDLLSIGVCIPC 60
           MANEQDEV A+ EAT   LTS  Q+AISP D+L        Q+PSY TKDL SIGVCIPC
Sbjct: 1   MANEQDEVAADLEAT--LLTSAAQQAISPNDELHVLNDAVPQMPSYVTKDLSSIGVCIPC 60

Query: 61  IIRLSGIERHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKG 120
           IIRLSGIERH YSFSLS PI+GSD +AIDDD DRN CCLCLGILQFSYIDDKG+F+KCKG
Sbjct: 61  IIRLSGIERHHYSFSLSVPIVGSDKKAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKG 120

Query: 121 VDDLAVSISEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180
           VDDLAVSISEMVKKEGHQIDSFSLE S+PSIILENEK VWLYLKRKYGSE WFQGKPPPI
Sbjct: 121 VDDLAVSISEMVKKEGHQIDSFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPI 180

Query: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKI 240
            LSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNG+S KR+QD  KMRKI
Sbjct: 181 SLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGSSNKRHQDSCKMRKI 240

Query: 241 VGSDEPSDIS--NCNAVADDCINPSQEV------VFPQETIKEPCHLALTCYRTSIYIVG 300
            G+DE  DIS  + N+VAD+C NPSQEV       FPQE I EPCHLAL CYRTSIY++G
Sbjct: 241 AGNDETLDISKFSANSVADNCFNPSQEVELSRCFEFPQEKINEPCHLALICYRTSIYLIG 300

Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
           RYIKYSRNVSQTRWVIG+ERMGEASVEEILGNNILP CRGDSYKFHAAGREDLDVRMLGS
Sbjct: 301 RYIKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGS 360

Query: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQK 420
           GRPFLVEIQNARLLPSEMI+NEI+SKIN S+NKLIGVRNLKVVGSEGWA VQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIVNEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQK 420

Query: 421 QYAALVWTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAES 480
           QYAALVWTSRPLED DLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWM+IEKVAES
Sbjct: 421 QYAALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAES 480

Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           SQYYLLHLCTQAGTYIKEFVHGDLGRTHPS+GSILSCRAEILQLDVTDIKMD HL E
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSMGSILSCRAEILQLDVTDIKMDGHLAE 535

[Close]
BLAST of CSPI04G26700 vs. NCBI nr
Match: XP_011654255.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis sativus] >KGN55479.1 hypothetical protein Csa_012687 [Cucumis sativus])

HSP 1 Score: 1039.6 bits (2687), Expect = 8.8e-300
Identity = 518/521 (99.42%), Postives = 519/521 (99.62%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RHSYSFSLSAPILGSDNEAIDD NDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHSYSFSLSAPILGSDNEAIDDANDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240

Query: 241 ISNCNAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI 300
           ISNCN+VADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI
Sbjct: 241 ISNCNSVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRWVI 300

Query: 301 GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS 360
           GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS
Sbjct: 301 GEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLPS 360

Query: 361 EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD 420
           EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD
Sbjct: 361 EMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDAD 420

Query: 421 LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI 480
           LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI
Sbjct: 421 LLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGTYI 480

Query: 481 KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE
Sbjct: 481 KEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 521

[Close]
BLAST of CSPI04G26700 vs. NCBI nr
Match: XP_008452922.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Cucumis melo])

HSP 1 Score: 1009.6 bits (2609), Expect = 9.8e-291
Identity = 501/523 (95.79%), Postives = 509/523 (97.32%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240

Query: 241 ISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
           ISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300

Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
           VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360

Query: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
           PSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420

Query: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
            D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVA+SSQYYLLHLCTQAGT
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKSSQYYLLHLCTQAGT 480

Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 523

[Close]
BLAST of CSPI04G26700 vs. NCBI nr
Match: TYK19969.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa])

HSP 1 Score: 1000.3 bits (2585), Expect = 5.9e-288
Identity = 502/537 (93.48%), Postives = 509/537 (94.79%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKI-------- 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI        
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKISNDCSLET 240

Query: 241 ------VGSDEPSDISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVG 300
                 VGSDEPSDISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ G
Sbjct: 241 IDDSIAVGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300

Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
           RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS
Sbjct: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360

Query: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQK 420
           GRPFLVEIQNARLLPSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420

Query: 421 QYAALVWTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAES 480
           QYAALVWTSRPLED D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVA+S
Sbjct: 421 QYAALVWTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKS 480

Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 537

[Close]
BLAST of CSPI04G26700 vs. NCBI nr
Match: KAA0064623.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa])

HSP 1 Score: 993.8 bits (2568), Expect = 5.6e-286
Identity = 501/553 (90.60%), Postives = 509/553 (92.04%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
           MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60

Query: 61  RHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
           RH+YSFSLSAPILGSD EAI+DDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI
Sbjct: 61  RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120

Query: 121 SEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
           SEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180

Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
           KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240

Query: 241 ISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
           ISNC  N+VADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300

Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
           VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360

Query: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
           PSEMIINEIQSKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420

Query: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHW----------------------- 480
            D LSISSLKDLKILQRTPIRVLHRRSPLEREKIIHW                       
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWLESFFNCTVKLQSTHASTKVTDN 480

Query: 481 -------MKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 522
                  MKIEKVA+SSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL
Sbjct: 481 VLLFHYRMKIEKVAKSSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 540

[Close]
BLAST of CSPI04G26700 vs. NCBI nr
Match: XP_038897427.1 (tRNA pseudouridine synthase Pus10 [Benincasa hispida])

HSP 1 Score: 946.8 bits (2446), Expect = 7.8e-272
Identity = 479/531 (90.21%), Postives = 495/531 (93.22%), Query Frame = 0 

Query: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQI--------PSYATKDLLSIGVCIPC 60
           MANEQD VQA+PEAT   LTS TQEAISP D+ Q+        PS+AT DLLSIGVCIPC
Sbjct: 1   MANEQDVVQADPEAT--PLTSATQEAISPNDEPQLLNHALPQMPSHATIDLLSIGVCIPC 60

Query: 61  IIRLSGIERHSYSFSLSAPILGSDNEAIDDDNDRNTCCLCLGILQFSYIDDKGDFIKCKG 120
           IIRLSGIERH+YSFSLS PILGSD EAID DNDR+TC LCLG+LQFSYIDDKG++IK KG
Sbjct: 61  IIRLSGIERHNYSFSLSVPILGSDKEAIDADNDRSTCSLCLGVLQFSYIDDKGNYIKFKG 120

Query: 121 VDDLAVSISEMVKKEGHQIDSFSLEASVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180
            DDLAVSISEMVKKEGHQIDSFSLE SVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI
Sbjct: 121 ADDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180

Query: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKI 240
           CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS SNGNSIKRNQ+G KMRKI
Sbjct: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASKSNGNSIKRNQNGCKMRKI 240

Query: 241 VGSDEPSDISNC--NAVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYS 300
            G+DEPSDISNC  N+VADDC NPSQEV FPQETIKEPCHLAL CYRTSIY+VGRYIKYS
Sbjct: 241 TGNDEPSDISNCDANSVADDCFNPSQEVEFPQETIKEPCHLALICYRTSIYLVGRYIKYS 300

Query: 301 RNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLV 360
           RNVSQTRWVIGEERMGEASVEEI+GNNILP CRGD+YKFHAAGREDLDVRMLGSGRPFLV
Sbjct: 301 RNVSQTRWVIGEERMGEASVEEIIGNNILPLCRGDNYKFHAAGREDLDVRMLGSGRPFLV 360

Query: 361 EIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420
           EIQNARLLPSEMIINEIQSKINSSENKL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALV
Sbjct: 361 EIQNARLLPSEMIINEIQSKINSSENKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420

Query: 421 WTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLL 480
           WTSRPLED DLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLL
Sbjct: 421 WTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLL 480

Query: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 522
           HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDYHFAE 529

[Close]
BLAST of CSPI04G26700 vs. TAIR 10
Match: AT1G20410.1 (Pseudouridine synthase family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 266/484 (54.96%), Postives = 333/484 (68.80%), Query Frame = 0 

Query: 39  ATKDLLSIGVCIPCIIRLSGIERHSYSF-SLSAPIL------GSDNEAIDDDNDRNTCCL 98
           A KDLLS+GVC  CI RL  +E       S+S   L      G D  +  + +    C +
Sbjct: 41  AVKDLLSLGVCERCIFRLVSVEAFDSDISSVSTSTLRSWLKSGDDETSASESSCSTICIV 100

Query: 99  CLGILQFSYIDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEASVPSIILENEKRV 158
           CLGILQF + D K + +K     D    I+++VK++ H+ DSF LE SVPS I+ENE+ +
Sbjct: 101 CLGILQFVFSDVKKELVKSDSSSDYVARITDLVKQDRHEFDSFGLEVSVPSTIMENERAL 160

Query: 159 WLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPK 218
             YLK KY +E W Q       +S KDALK+ +   L+  L  +S SS   IRLTY+  K
Sbjct: 161 LSYLKGKYSTEVWLQRDK----ISVKDALKVLLLDQLKASLGAESDSSSFHIRLTYT--K 220

Query: 219 ASNSNGNSIKRNQDGFKMRKIVGSDEPSDISNCNAVADDCINPSQEVVFPQETIKEPCHL 278
           AS+    + +   +  K RK       +D  N      +CI+ +       E + EPC  
Sbjct: 221 ASDEAQGASETTHES-KRRK-------TDAEN----GSNCISENS-----FEKVYEPCIF 280

Query: 279 ALTCYRTSIYIVGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHA 338
           ++ C R  I+  GRY KYSRNVSQ+RW+I +ERMGEASVEEILG NILP C GDSYKFHA
Sbjct: 281 SVHCNRMPIFFSGRYFKYSRNVSQSRWIIDDERMGEASVEEILGGNILPACLGDSYKFHA 340

Query: 339 AGREDLDVRMLGSGRPFLVEIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEG 398
           AGRED+DVRMLGSGRPFL+E+QN+R LPSE  + E++ KIN SE KL+GV++LK +GS+ 
Sbjct: 341 AGREDIDVRMLGSGRPFLIEVQNSRQLPSEQSLKEVEEKINKSEKKLVGVKDLKCIGSQC 400

Query: 399 WALVQEGEAEKQKQYAALVWTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREK 458
           WA+++EGEAEKQKQY ALVW SRPLE+ D  SIS LK+LKILQ+TP+RVLHRRSPL+REK
Sbjct: 401 WAMMREGEAEKQKQYVALVWISRPLEEKDFNSISCLKELKILQKTPVRVLHRRSPLDREK 460

Query: 459 IIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVT 516
           IIHWMK+EK+   S Y+LLHLCTQAGTYIKEFVHGDLGRT PS+GSIL CRAEI+QLDVT
Sbjct: 461 IIHWMKVEKIKGHSHYFLLHLCTQAGTYIKEFVHGDLGRTTPSMGSILGCRAEIIQLDVT 501

The following BLAST results are available for this feature:
BLAST of CSPI04G26700 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3MIT23.2e-6637.05tRNA pseudouridine synthase Pus10 OS=Homo sapiens OX=9606 GN=PUS10 PE=1 SV=1[more]
Q9D3U01.9e-6336.44tRNA pseudouridine synthase Pus10 OS=Mus musculus OX=10090 GN=Pus10 PE=1 SV=1[more]
Q6ING24.7e-6234.88tRNA pseudouridine synthase Pus10 OS=Xenopus laevis OX=8355 GN=pus10 PE=2 SV=1[more]
Q9VPK74.5e-3629.62Putative tRNA pseudouridine synthase Pus10 OS=Drosophila melanogaster OX=7227 GN... [more]
A1RX331.7e-3536.73tRNA pseudouridine synthase Pus10 OS=Thermofilum pendens (strain DSM 2475 / Hrk ... [more]
BLAST of CSPI04G26700 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3784.3e-30099.42tRNA pseudouridine(55) synthase OS=Cucumis sativus OX=3659 GN=Csa_4G653400 PE=3 ... [more]
A0A1S3BUF44.7e-29195.79tRNA pseudouridine(55) synthase OS=Cucumis melo OX=3656 GN=LOC103493791 PE=3 SV=... [more]
A0A5D3D9062.9e-28893.48tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5A7V9212.7e-28690.60tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1IEB14.3e-26085.85tRNA pseudouridine(55) synthase OS=Cucurbita maxima OX=3661 GN=LOC111476467 PE=3... [more]
BLAST of CSPI04G26700 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011654255.18.8e-30099.42putative tRNA pseudouridine synthase Pus10 [Cucumis sativus] >KGN55479.1 hypothe... [more]
XP_008452922.19.8e-29195.79PREDICTED: putative tRNA pseudouridine synthase Pus10 [Cucumis melo][more]
TYK19969.15.9e-28893.48putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa][more]
KAA0064623.15.6e-28690.60putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa][more]
XP_038897427.17.8e-27290.21tRNA pseudouridine synthase Pus10 [Benincasa hispida][more]
BLAST of CSPI04G26700 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G20410.11.6e-14254.96Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.2510coord: 279..403
e-value: 1.1E-39
score: 137.4
NoneNo IPR availableGENE3D3.30.70.3190coord: 404..514
e-value: 6.0E-41
score: 140.4
NoneNo IPR availablePANTHERPTHR21568:SF1BNAC07G15400D PROTEINcoord: 38..516
IPR039894tRNA pseudouridine synthase Pus10-likePANTHERPTHR21568UNCHARACTERIZEDcoord: 38..516
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 400..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G26700.1CSPI04G26700.1mRNA
CSPI04G26700.2CSPI04G26700.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0003723 RNA binding
molecular_function GO:0106029 tRNA pseudouridine synthase activity
molecular_function GO:0009982 pseudouridine synthase activity