CSPI04G26680 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G26680
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Alpha-amylase
Location	Chr4: 23685952 .. 23693440 (+)
RNA-Seq Expression	CSPI04G26680
Synteny	CSPI04G26680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATGGTCAGAGACGGAACTCCACATCCCGCACCCCCTCGATTGGTTCTAGCCAAGAATCTCGAGCCCACGCGGCTGCAATTCAATTGCCTCATTGCAAATGGTTCGAACAGATAAAATCGTTTCCCTGAACCAGCAGAACCAAGAACGTCGAACCGCGAAGTGATTGCTTCTTGGTAACGTTTTTCATTATTTGCTAAGTAACTTTTATTCATCATCGCCTTATGTTCTTGCGATGTTCAAATGCGGAATTCTAATCACATTACTTAGTGAAGCGTTTTGGTTGTTTAGTTATTTTCCCATTAAATATGAGTACACGACGAATCATTCTCAAAGCATCTTTCGTTGTAATTGCGTTCTTCTTTTACTCCTTTTGAATTGAGGATTATTGCTAGCTAATCTTATCTCTTTCTTGAATTTTTCAGCGGTAAATGTCTTCGCGCCAATCTGAAACTGTGATTTCAGCTGATTAATTCGTATTGTCAAGGTTGTGAACGCATCTGCTGCATAGCTATTTAGAAGTTGATTTTAGACAATTTTAAGAAGTGTTTATAGATTATCCAGTTTCAAATGGAGGAAACGATTCTCAAAATGAATTGTCCACACCACGTCACTAAGAATTCTGTTATAATTCTAGCTCGTTTTCTTATTGTCTTTTCCGTTGTTGGAGTGGCCATCTGGATAACGAAGTTAAGATCACACAAGATGATTGAGGTTACACTTTGCAGATTGTGATGCTTTTTCATTTTGGTTGTAGGACTAGGACGTCCCTATATTCTGCAACCAAGACGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAACGTATGCTTCTTGTTTAATTTGGCAATTGTTTCCTTGATGAGTTTTCTGCATCAGTGTGATATGCTTTTAGTTATAAATTTGTTGGAAATTAGAATATACATTATGATATACAAATTACTTATTTATGTTGGAATTCAGGTTTTTGATGAAAGCTACGAACAGACTGATACTGGTATGGTTTTTTCTTTGTTTTGACATTAAATTTCACATCATCGTATGCTCACGTCAAACTAGTGTTTGTGAAAAGATCATACCTCTCTTGGAAGTGATTTGAAAAGAAATGTGAAAAGATCGTATGCTTAAAATTTGACTCAAGGATAAGAAATTTGAAAAGAAATAGATGCTTCTTAGGTGTGAGTACGTTGCAATATTTGCTTTTAATTTACATAAAATTTGTGGTTTATGAAAAGGATTGATACACGTGGCCTTGAAATCAGAGCTGGTTTTTTGCTATGTAAATTTTCTTTTGTTTGAAATTATCTTATCATTTTAATAATTTCAGTGTTTCTTTATGGTCACCTGTAGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGATGTTACAATTTCAGCTTTCTGATTTTTCTCTAGAGCAGAGGTCAGCAGGAAGGAAAATATTATGTTGCTCTTAATTGAATTGAAGTCTCGGAAACAAAAAAAGAAAAAATGTTTGCCGTTTGAAATCCATGCTCGATGTTGTTGATTCTTTTAATGTTAATCGGTTAGATCAAATCTCCAGGTCATGTTAAACTTATGAGAGAAATTTTGCACCTATAATTTCCATTGTAATCACAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTCAAAGGAACAAATTCATTTGAATTTTTATGTTCCTTTCACGTATATGCGTGCATAACTCTTGATATTTTCTTTACAATTTCAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAACTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGAACGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGTATTTTTCTGTTACAAATTCCAAGTGTGCTCATAATTATAAGAAAACCTTTGGTCTTTAGTCTGTGGTATCTGTTCTAGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTGTCCTTCTCTGTTTGTGTGTGTGTGTATTTATATTGTGGGGTGGCTGGGGCGTTACAGAGGTTCAAGGATATATTGTTCTGAAGATGACAAGATCCCTTCTCCTGTTTCAAAGTTTCACTGTATCATCTTCTCTACATTCGGCTTGTCTTTCCTTTTAAGGAGTTCTTCAATGGATTATAACATACATTTGCCGTTTGTTTTTATTTTTTATGTTTTAACTCAGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGGAAAGCCAATGCTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGTCATGACTTGGACTATAATCAAGGTAACATGATAATGCATTATGTTGATAAATTGAAATCATGTTTTCAAGTTTCTGGATTTTTAGTTTTTCTTAATGGTTTTGTCCGTGAATAATATCCTATGTTCGTCCTATCAATTCATGTGTTCCATATTGATGATTACAACTCTAAATTCAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGTATTGGTGTTGCGTGAGGCCATTGCTAACTCCCTTCCCTCCTCAAAATTGTAATACTACGGACCTCCAATCTTAATTACTTAGAATAGAAAGGATGAAGTGACAGCCATGTCATGGTTTTTTTTCCCTCTAGATCAGTGGATCTACAATGTTTGACTTTTCACAACTCTTTCTATTTGCTTTTTTCTCCTTCTTTATTGAGTGATGCTTGGGTTTCGTTAATATTTGCTCTTGCTAGTACATTGCTTGACTAACATGCATTTGTTTCCTTGTTTGCTTCGTAATAAACATAATATAATTAATTTTGCTTGTAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAATCATGATACCGGTTCAACGCAGGTACCACTTCTTCAAATCTGTTTGTGAAATTATTGGTTGACAAATTTCAACATTTGTTTTCATTGCTCGTTGTGCATGTGCGTTATAAAATTGTCTCAACTTGACCACGTATCCATGTCCTTTTATTTGGATTAATATTTGAGAACACGTGTTTGGAAGTCATGCCTTGTATTGAGGATTGAGTGTGATCAAACCATACATGAAAATGTCATAGGAAGCAAGGAGAAAAGTAGTATCGATGCATACCTGAATGAAGTTGTAATGTCTGGTTGTTCTACACAGCTTCTATATGACCAAATATGGTGCCATTCAGAACAGCTACTGGACTCTGTTCCATTTGCCTGTGTTGCTATTATCTTTTGTAAGATGCGTATGTGTTTTTTGCAACATGTAACTGAGATTTATTGGCCAACCTTTCTTTTATATAGGTTTGTTCAAATTGAACTAATTCAAACGTAAATAGTTCTAGGCTTCTAGCAATCCAACTCTGCATCTTAATAAGTAGATAGCTTTAGATTATTATTTGGAAACTCTTAATTAATTTTTTTTCCTAGGTTGAAATCAAACATCAGGTGTGCTTTCAAGTATAGATATTGACTTATGCAGATCATGACAGTTAATGCAACTCGACCTGCTTGGCAAATGAACTATATAATTCTTCATATGTATTTTCTTAACATTTATATAAGCTGAAATTGTACCATTAGAATGTTGGTGACTCCACTAACCTCAGCATACCATTGTTCTTGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGTATACAAGTGTGGCATATCATTCCATTTGCAATCTCTTTATTGCTTATAGTCTTATTCTTGCTGTGCTGTGGCTTCTTCGTGGTTTTTTGAAGCTCTCAATTGTTTGGTCCAATTACTCATTGTGTAAAATCTAATTACCCCCCATCAGGTGGTCCTCATTGTTCGCTGTGTGTTTTGGAAGCTTTCAATTGTTTGTTTCAATTGATCCTATGTACTACTCTCTGTCTACATTTGAAGTTCGTTTCCTACTTTTAGCTGCAAATTCTATGGCTATAACTGTTCAGTATGCATTTAAAAAAAAAACTGTTCAGCACACTAAGCGGTCAATCTTTGATGTTTCTGGGTGAATTTCATCGAAAGAACTTCATAATTATTTTCTAGCCTTACAATTGTTACTGCAACTACTTTTATGCTTGGCCTAGAGTCTTCACTTACCTTCCAGTAAAAAAAAATTCCATCATGCATCTATTTTCTGATTCCTGGAGCTTTGGTTATTCCCTGCAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAGTAAGATTAGTATGTTGCATAGTCAATAATGCTTCGATAGAGTTTGTTATCTTCTATTCAAGCAAACTTCTCATAGTACTTAAATTTTATTTTTCTTGCAGATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCATTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAAGGTTTGGAAAATTTACCTGCCTTCCCCAACCTTATATGCTTCTTTTTGTGCCATCGGGGACTACCTGTGTAAACTATAAATTTTCATAACAAGTTGGCTACTCAAAGTATAGTATCATAAATAAAATCACTGTGGATGAAATGTATTTTCACATAGTCTAGATTAAATCACATCGCTTATATTATTGTCTTCTGTGTAATTCTAAATACACTGTATGGTTAAGTCTGTTAATAGAAATGATTGTTCTCTTCAAGCAAACTAACCTTCCCCATTTGGAGCTTTGAGTGCTATATAATATATATGGTGGAGTGCCTCTCGAACAAAGTTTGATGTACTTGGATAGTTGGAACTTGGTAGATAGTTATTCATAATTTGAATTATGTAGATACGTAGTATGTTTTAATTTATAAACTAAATATAAAGTCATGGAGTGTTTACTTCTTGGAGTACAGTGATTTATAAACTTAGAATTGGAATATAATGAAAAGGAGGCGCTTTGAAGTCCTCTACGAGATTGGGATGGTTTGTGGAATTGAGCTATGAAGAAATTTGAGGAGTTACACGTCCTTAGGAAGGCTGTCTTATTCTTGTTCGGGAACTTTAATCCGAAATTTTTTTTCTTTATTGTCTAAACTAAATATAGTCGAATCATATTAAAATATTTTGTTTGTAAAAAAATTGTCCTAAATTACAAACATGTAAGTAGTATTTGAAACTTTTATTGAGTACAATATAATCTCTATGGGCCATTCTATCACAAATTTTGAAATGAAGTAGAATACCTCTCTGCCCAGTCCTTATTAGGGTTCATTTCTTATCACATGTAGGCCTTCCCTTCATCAAGTTTCTATTCACTACGCCTACTGATGTCTTTATGTGGGCGTAAGATTGAGCAATCATTGATTCTTTGGATAAATCTTAAGGCATGGAGGAAGACTAAAGTTAATATGAATGTGCGAAGCTGTGCATAGGGGATCCACTGTATTAGAACTTTAAATTAAATTTGGTATGTAGATTGATTGGAAAAGTAGGGAGAGAGTTTAGGGATAGCAAACTGAAAAATCAAACATGGACATGGTTTGATCGGCTCAGTTTTATTTAGTAGGTCGATTCGATTCAATTTCAGTTGGTTGGATAGTTGGTTATGATATATCTGATATTCCAAAAGGTTAGTCTCGGTATCGGACTAAATGGAGTCGAACTAATCTGTTTGTTCTCTTAATTCTTAAAGTGTGTGCCACATAGCAGAAGTGTAGGGAAGAGACGACAAAGCATCGCCATCACAATACCACGGTCAATCCCCTCATCATTTTTGCTTTGAAACTCCAAAAGATACACAGAACAACGCAACCCTTCCACGGCTTCCCTTAATCTTGACCATCATCCCGTATATTGTATGTATTAGTTGGGCCATTATCATGTTTATCAACAGCGAGTCTCTATAATTTCTTATGTAACCTTATCCTGTGTGGACACTACCTTGTCCCAGTCCTCAAACTCTTTAACTTTGTTTTACCTGTCACTTGATTATTCGATATAGGCTGGTTAATTTATTCACAAGTGGTACTAATTAATCACAGTAGCTAGCTAGTTTGCTTTTATAAAATTGACTAGTAAATATTCATGATTCATCTACTAGCATCAGCACTAGCACTTTCTATATATCTATTGGAAATTATGAATTTTTTTATTATTCAGAAACACATTCAAACTACTGTCTGAAGCAGCAGTAAATCTGGCATTCATGTGACAGAATATTATCTACCAGTAACATGGCTGATTGCAGCAAAATGAGTCTAAACTTTTTTATACCAAGCATTCATTCTTTTGTTCTCTAACTGCCTTTTCAGATTCTCTTCGTGCAGGATTATAAAGTAGATTTTTCTTGAGGCTCCAAATGCAGCAGCCAGCGATGAACTCTTTCTCTCTGACGTCCACCGCCGGAGCATAATTGGGGATTCTAAGGTAAAGTTAAAAAATAAAAAAATTAAAGAACTTATAACTGTTGATTTTTTTATTATTTTAATTATTGTCGTTGAGACATTTTCATTACGGCAGATATCCACTTTTTAACCACAACGGCTCGCAATCATTGAAAACAGTATGGGAATCCCATCCAGTATCCACCGAGAGCTGTGCAGTAAACAAAAGTGTAAGGCCCACTTGAACAATAACAAAATGTTGAATAACAAAATGTTGTTGGTTTTTACTGCTTGCTTGCTGCCTGATTCTTTGAATGAAATTCAACTTTGTGTTTTGGTCAACCGTCCTGAATTTTAGTTAAGAAAGAGAAAGAAAATTAGATGCTTATAAGCATGCGTAAGTGAAGTTTTCTAGTGGTCGTCCCTACAAACAAAATGCCATATACACGTTTCCCCAAGGAACATGGAAGTTGTTGGAATTTTTGCACCCCAGCCCTAGCAACGAACCACATGATCAAAGTCCTTTCCAAGATTCAAGTAAAGATCACATGAATGTTTTCC

mRNA sequence

AAATGGTCAGAGACGGAACTCCACATCCCGCACCCCCTCGATTGGTTCTAGCCAAGAATCTCGAGCCCACGCGGCTGCAATTCAATTGCCTCATTGCAAATGGTTCGAACAGATAAAATCGTTTCCCTGAACCAGCAGAACCAAGAACGTCGAACCGCGAAGTGATTGCTTCTTGCGGTAAATGTCTTCGCGCCAATCTGAAACTGTGATTTCAGCTGATTAATTCGTATTGTCAAGGACTAGGACGTCCCTATATTCTGCAACCAAGACGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAACGTTTTTGATGAAAGCTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAACTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGAACGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGGAAAGCCAATGCTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGTCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAATCATGATACCGGTTCAACGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCATTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAAGGATTATAAAGTAGATTTTTCTTGAGGCTCCAAATGCAGCAGCCAGCGATGAACTCTTTCTCTCTGACGTCCACCGCCGGAGCATAATTGGGGATTCTAAGATATCCACTTTTTAACCACAACGGCTCGCAATCATTGAAAACAGTATGGGAATCCCATCCAGTATCCACCGAGAGCTGTGCAGTAAACAAAAGTGTAAGGCCCACTTGAACAATAACAAAATGTTGAATAACAAAATGTTGTTGGTTTTTACTGCTTGCTTGCTGCCTGATTCTTTGAATGAAATTCAACTTTGTGTTTTGGTCAACCGTCCTGAATTTTAGTTAAGAAAGAGAAAGAAAATTAGATGCTTATAAGCATGCGTAAGTGAAGTTTTCTAGTGGTCGTCCCTACAAACAAAATGCCATATACACGTTTCCCCAAGGAACATGGAAGTTGTTGGAATTTTTGCACCCCAGCCCTAGCAACGAACCACATGATCAAAGTCCTTTCCAAGATTCAAGTAAAGATCACATGAATGTTTTCC

Coding sequence (CDS)

ATGGGTGACCATGATAACGTTTTTGATGAAAGCTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAACTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGAACGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGGAAAGCCAATGCTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGTCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAATCATGATACCGGTTCAACGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCATTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAA

Protein sequence

MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK*

Homology

[Close]

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 1.4e-205
Identity = 317/413 (76.76%), Postives = 371/413 (89.83%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI A KP+ S+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+I   IDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRS+V++L+A S+ Y+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-114
Identity = 193/394 (48.98%), Postives = 267/394 (67.77%), Query Frame = 0


Query: 23  NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A KP  ++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRSSVKILEARSDHYSAIIG 382
           GYAYILTHPG P VF+DH++    S    ++ +R  Q ++ RS V I ++  D Y+AII 
Sbjct: 793 GYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIID 852

Query: 383 EKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 410
           EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 EKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot
Match: P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.4e-100
Identity = 187/413 (45.28%), Postives = 258/413 (62.47%), Query Frame = 0


Query: 19  AVIRNGREILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLP 78
           A + +G ++LFQ F+WES K    W+  +  KV DIA +G T  WLPPP+HS++ +GY+P
Sbjct: 19  AGLASGHQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNEGYMP 78

Query: 79  QNLYSLNSS-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIL-- 138
             LY +++S YG+   LK+L+  +    V+AIADIVINHR        G++  F+G    
Sbjct: 79  GRLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSD 138

Query: 139 --LPWNEYAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGF 198
             L W  + +    T  S G  N  TG +F   P+IDH    V+R++  WL WL+S +GF
Sbjct: 139 GRLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGF 198

Query: 199 QDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWID 258
             +R DFARG+S +  K YI+   P L++ E WD+    G    +Y+QD HRQ ++NW+D
Sbjct: 199 DAWRLDFARGYSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVD 258

Query: 259 GTGQLSAA---FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGS 318
             G  ++A   FDFTTKG+L  AV  +LWRL D QGK PGVMGWWP++A TF+DNHDTGS
Sbjct: 259 KVGGAASAGMVFDFTTKGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTGS 318

Query: 319 TQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWG--DSVHDEIIDIRKHQDINSRSS 378
           TQA WPFP++ +M+GYAYILTHPGIP +FYDH + WG  D +   ++ IRK   I + S+
Sbjct: 319 TQAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQI-AALVAIRKRNGITATSA 378

Query: 379 VKILEARSDHYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 411
           +KIL    D Y A I  K+ +KIG     G+  P+G  +  +  G  YAVW+K
Sbjct: 379 LKILMHEGDAYVAEIDGKVVVKIGSRYDVGAVIPAG--FVTSAHGNDYAVWEK 428

[Close]

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot
Match: P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 3.4e-100
Identity = 177/402 (44.03%), Postives = 254/402 (63.18%), Query Frame = 0


Query: 26  EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           +ILFQ F+W+S K    W+  L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 86  SS-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNE 145
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 146 YAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 206 ARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLD-YNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++  +P   + E W+S +Y+G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 266 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+ +LWR+RD  GK PG++GW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 326 HIMEGYAYILTHPGIPTVFYDHLYEWG-DSVHDEIIDIRKHQDINSRSSVKILEARSDHY 385
            ++ GYAYILTHPG+P +FYDH+++W      + +   RK   IN+ S +++L A SD Y
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDWNLKQEINALAATRKRNGINAGSKLRVLAAESDMY 388

Query: 386 SAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 411
            A++ E++  KIG     G+  PS  ++ +   G  Y VW+K
Sbjct: 389 VAMVDERVITKIGPRIDVGNIIPS--DFHIVAHGNDYCVWEK 428

[Close]

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.0e-99
Identity = 181/403 (44.91%), Postives = 253/403 (62.78%), Query Frame = 0


Query: 26  EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           ++LFQ F+WES +    W+  L  KV +IA +G T  WLPPP+HS++PQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 86  SS-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIL----LPWNE 145
           +S YG+E  LK+L+       V  +ADIVINHR        G++  F+G      L W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 146 YAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 206 ARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++   P   +GE W S  YNG      NQD  RQ ++NW++G G+ + 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 266 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+ +LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++
Sbjct: 266 AFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSD 325

Query: 326 HIMEGYAYILTHPGIPTVFYDHLYEWGDSVHD--EIIDIRKHQDINSRSSVKILEARSDH 385
            +M+GYAYILTHPGIP +FYDH+++W +  H+   + +IR    I++ S++ IL+A  D 
Sbjct: 326 KVMQGYAYILTHPGIPCIFYDHVFDW-NLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385

Query: 386 YSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 411
           Y A+I  K+  K+G     G   PS  ++ +   G  Y VW+K
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425

[Close]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL
Match: A0A0A0L5R6 (Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 1.8e-253
Identity = 408/410 (99.51%), Postives = 409/410 (99.76%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYE+TDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS 360

Query: 361 SVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SVKILEARSD YSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

[Close]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 2.6e-236
Identity = 387/413 (93.70%), Postives = 395/413 (95.64%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 2.6e-236
Identity = 387/413 (93.70%), Postives = 395/413 (95.64%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.4e-234
Identity = 387/417 (92.81%), Postives = 395/417 (94.72%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 295

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFL
Sbjct: 296 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 355

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKH
Sbjct: 356 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 415

Query: 361 QDINSRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           QDINSRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 416 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 471

[Close]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL
Match: E5GCD3 (Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.4e-234
Identity = 387/417 (92.81%), Postives = 395/417 (94.72%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           QDINSRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

[Close]

BLAST of CSPI04G26680 vs. NCBI nr
Match: KGN55476.1 (hypothetical protein Csa_012097 [Cucumis sativus])

HSP 1 Score: 884.4 bits (2284), Expect = 3.7e-253
Identity = 408/410 (99.51%), Postives = 409/410 (99.76%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYE+TDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRS 360

Query: 361 SVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SVKILEARSD YSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

[Close]

BLAST of CSPI04G26680 vs. NCBI nr
Match: XP_011654252.1 (probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylase 2 [Cucumis sativus])

HSP 1 Score: 879.0 bits (2270), Expect = 1.6e-251
Identity = 408/413 (98.79%), Postives = 409/413 (99.03%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYE+TDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI   IDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRSSVKILEARSD YSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 413

[Close]

BLAST of CSPI04G26680 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 827.4 bits (2136), Expect = 5.4e-236
Identity = 387/413 (93.70%), Postives = 395/413 (95.64%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of CSPI04G26680 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 827.4 bits (2136), Expect = 5.4e-236
Identity = 387/413 (93.70%), Postives = 395/413 (95.64%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of CSPI04G26680 vs. NCBI nr
Match: ADN34132.1 (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 821.6 bits (2121), Expect = 3.0e-234
Identity = 387/417 (92.81%), Postives = 395/417 (94.72%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQTDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           QDINSRSSVKILEARSD YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

[Close]

BLAST of CSPI04G26680 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 716.8 bits (1849), Expect = 9.6e-207
Identity = 317/413 (76.76%), Postives = 371/413 (89.83%), Query Frame = 0


Query: 1   MGDHDNVFDESYEQTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI A KP+ S+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+I   IDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDHYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 411
           SRS+V++L+A S+ Y+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of CSPI04G26680 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 414.1 bits (1063), Expect = 1.3e-115
Identity = 193/394 (48.98%), Postives = 267/394 (67.77%), Query Frame = 0


Query: 23  NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A KP  ++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIIDIRKHQDINSRSSVKILEARSDHYSAIIG 382
           GYAYILTHPG P VF+DH++    S    ++ +R  Q ++ RS V I ++  D Y+AII 
Sbjct: 793 GYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIID 852

Query: 383 EKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 410
           EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 EKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of CSPI04G26680 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 342.4 bits (877), Expect = 4.9e-94
Identity = 179/402 (44.53%), Postives = 251/402 (62.44%), Query Frame = 0


Query: 27  ILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 86
           +LFQ+F+WES K +  ++ +L + + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 87  S-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNEY 146
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L W+  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 147 AVTSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
            V        G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ R
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 207 GFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGH-DLDYNQDGHRQRIINWI-DGTGQLSAA 266
           G+++   K Y++   P  ++GE WD   Y G   LDY+Q+ HR  +  WI +  G +  A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 267 FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AV+ +LWRL+D QGKPPG++G  P  AVTF+DNHDT  T   W FP++ 
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 327 IMEGYAYILTHPGIPTVFYDHLYEWG--DSVHDEIIDIRKHQDINSRSSVKILEARSDHY 386
           ++ GY YILTHPG P +FY+H  EWG  +S+  +++ IR    I S SSV I  A +D Y
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWGLKESI-SKLVAIRNKNGIGSTSSVTIKAAEADLY 386

Query: 387 SAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 411
            A+I +K+ MKIG     G+  PS   + LA SG  +AVW+K
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:

BLAST of CSPI04G26680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFG1	1.4e-205	76.76	Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1	[more]
Q94A41	1.9e-114	48.98	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1	[more]
P00693	3.4e-100	45.28	Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1	[more]
P27932	3.4e-100	44.03	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2...	[more]
P27934	1.0e-99	44.91	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]

BLAST of CSPI04G26680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5R6	1.8e-253	99.51	Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1	[more]
A0A5A7VG02	2.6e-236	93.70	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440...	[more]
A0A1S3BW59	2.6e-236	93.70	Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1	[more]
A0A5D3D8R2	1.4e-234	92.81	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ...	[more]
E5GCD3	1.4e-234	92.81	Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of CSPI04G26680 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN55476.1	3.7e-253	99.51	hypothetical protein Csa_012097 [Cucumis sativus]	[more]
XP_011654252.1	1.6e-251	98.79	probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylas...	[more]
KAA0064621.1	5.4e-236	93.70	putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008452915.1	5.4e-236	93.70	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
ADN34132.1	3.0e-234	92.81	cytosolic alpha-amylase [Cucumis melo subsp. melo]	[more]

BLAST of CSPI04G26680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76130.1	9.6e-207	76.76	alpha-amylase-like 2	[more]
AT1G69830.1	1.3e-115	48.98	alpha-amylase-like 3	[more]
AT4G25000.1	4.9e-94	44.53	alpha-amylase-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 41..58 score: 21.79 coord: 290..302 score: 53.54 coord: 103..114 score: 52.04 coord: 187..198 score: 35.54
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 352..410 e-value: 1.3E-26 score: 104.4
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 354..409 e-value: 6.3E-21 score: 74.4
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 26..363 e-value: 2.7E-20 score: 83.4
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 47..114 e-value: 2.0E-8 score: 34.2
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 26..355 e-value: 4.2E-114 score: 383.6
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 22..410
None	No IPR available	PANTHER	PTHR43447:SF18	ALPHA-AMYLASE 2-RELATED	coord: 22..410
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 27..362 e-value: 2.29372E-159 score: 448.979
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 356..410
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 356..410 e-value: 6.6E-18 score: 66.6
IPR013775	Alpha-amylase, plant	PIRSF	PIRSF001028	Alpha-amylase_plant	coord: 7..410 e-value: 2.0E-175 score: 581.7
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 32..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G26680.1	CSPI04G26680.1	mRNA
CSPI04G26680.2	CSPI04G26680.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding