CSPI04G26060 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G26060
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein FAM33A
LocationChr4: 23285017 .. 23289527 (+)
RNA-Seq ExpressionCSPI04G26060
SyntenyCSPI04G26060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGCGAAAACCCTAGCGGAGAGAGAGTATGGGGATCGATCATCATCTTGATCAACATCATCAAGCGGCCGATGGATTGATGAATTTGTTCACGAAGGCCAATCTCGATCTCTCAGTAGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATCTATCCTGAAAGCGTAAGAGCAACACGATCTTTTTCCTTCCTTCCTAGCTTCTTCTTCTTCTTCTTCTTCATTTTATTATTCTCGTTGATTGAATACAGTAATTAGGCACTGATTAGTTTAAGGATGAACTTCTAGGGCTAGAAATGAGTATAGAACTGTTTACGTCTTTCTAAGCTGTGAGTGTTTCACAGTGCTAGTACCAGATCTTCGAGCGCTTACGTAGAATGACTTCATTTCTACAGCGCAAGAGCATTATAGGCTTTCTCTATGTTTTTTATCGAGACACTATTAGATGGAGTTCAAGATTTGGATCTCAATGTTAAACTATTTTGTTATCTATTTGCTGTCGTTTCATTGTCCTAATACAAGAAATCGAGCTTAATGTAGGATTATTCAATTCGACAGCGTAGAAGGAAGTCACCTTTCCTGTCAGTAAGAGCTTCATACATTTAACTTTTCTTCTAATTCTTCTTCTTCTGAGCTGAATCAAGATATATTTCATTATCTTTTGGTTATTCACATTTCACAACAGCATCACAAGATATTGACCTGCTCATGAGCTAGAGTGGAATTATTTCGGTTTACAGTGTAAGAGCAATTAATCTATCCTGCTGTTCTATATGATTTGAGGAGGAAATAGTAGCTAGATCTCGAGCTTAATGAAGAGTTATTTCATTTTTCAATTAGAACGGCGGTACTATTGTATGATTTTATGCTGAATGTAGAATTGTTTCTTTAGCTCTTGCCAGTCAGTTTATTTCATACCGCTAACCAGTAATTTATTGTGGTTCTTGATTTTAAAAGGTTTGTTTCGCTCTATAAATTTGTGGATCCTGTAATTTTTAGGTTGAACTTGGAAAATTTGTTCACCCATAGTTTCTCTTCATTTTTGAAATTCTGATTCTATTCAGCTTTTTGATGCTTAACGTCTGATTATTTCCGTTTGTTTCTCAATAATCTTTTTTGGCGGTTAAAATGCATTCAAACACAATAGTGACCAAGGTTATTCAGTCTTTGTTTCACGGTTGAGATTAGAAAGTCCTCTTAGAATATGTAATGGTGACGGGGATGAAATATCTCAATGTACCAAGTATATCTCCTTGCAGCACAAAGATAACCAATGAATAAAGTGGAATTCAATCTACTATTTTCTTCCATATTCTTTCTGGATAATAGGAGTACTAATTTGTTATTGTTCGGTGGTGATTTTATCCAAGATTTTGTGAGTTTTCTGCAGGCGAATCCAATGAAGTTAGTTTCTAGGATAAAGAAGATACAGGATGATATATCAACATTGAAAGAGCAGTGTCGTGAAGTTTTAATAGCTAAACAGGTACTTTCTTCATTTATTATCTCAAGAAAAGTATTGGCTAGAAGAAACAAGTTTGAACTGCTAGATCATGCTCTATGTATATGAACTTTTTTTTTTCTTATAAGAATTAGGTCTGTCTCCATTTCACTTAGATTCTGTGTGATGGAATGTTGATATGATGCATATTATATCTAAATAAATGAAATATGATGTTTGGTAATGTGATGTCTGATGATAATATAGGAGAATGTATTTGGTTGGTGAAAATGTGATTTGTATTTTTTTATATATAAGTTAAAGTCTAATTGTATGTTTCATTAGATTTATTTCAAGAATATTTTGTTTAGTATATTACAAACATTGAACTTAGAAATTATTTATGGTTAATAAATTTTCATTGAAATATCTAAATAAATATTTTGTTTAATCCAATTTTCTTTACGCAGTCCTCACCTTTCTTAAACAAGCATTTGGATTTGTAGTCAAATTCTAAAAACAAAAACTATTCTTTATTTAGTGTTCGTTTTGTATTTTAAAAACATGAGTAGAACATAGATAACAAAACAAAAATACTTGGAATTGTAGTGTTTATAAGCTTAATCTTTAAAAATGACATTCTAATGATATGTTTAATAATGACTTTTTATTCAAAGCCAAACCATGTTTAGAAAACTCGACAAACAAAAAATGTTAAACGTCTTGGTAGTTGGTGCCTCTTCTTCTCTTTATCGTAGGACAAGGATTAGAAAAGACGTGTACACTATTTTTACTTGTTTTAGAACTGAATGCTCTGTTTGATACCTATTACTAATTTTTTTAGGAGGCATGATATGGTGACATGTACGGTAGTTATTTTTGTTCTGGAAGTGAAACTGAGTAGTTCCAATACATACTGAAACGATGATATGCTGTTCAAATGCAGGATGTAATCGATCAGGCCCGGACGGTTCTTCTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCATACCCTTTGTTAGTGATCTCGATGATCCTGCATTTTCGGAATTCAAGCAGGTATCCATCATCAATGTCCCTTGATTTGCATTTTGCTGTGGTCAGTACTGGTGCAATGCATTGATTATTCCTAAGTCTTGAGCCTGAATTGCATTTCGTGGTTAATATGCTTATGCAAACTATTGCACCTTTTAGTTTTGATGTCACAATGTACTCTTACTCTTATTTTAGGCATGTTTGGAATTGATTTTGAAATGTTCAAAACCACTCTAAAACATGCCTTTAATGACTTGAAGTTAATTTAATATTTAACTTTACTCTTTTAAATAAATTTTTTATTTCAATAAAGTTAGTTTTGAATTATAAAGAACATGTTTTATAGTGATTTTAACCATTTTAAAATCACTCCCAAAACACTTTTTTGTTTTTGTTTTTAATGTACCTGTTGTCTTAGGTCATTTAAGTTTCCACTTAACATCTTACTAAACTATCGTTCTTCCTTAGCTATTAAAAAGAGTAACACAGTGAATTACTAATAAGTTAGTTTCTAGGATAAACACTTTTGTCTCACGTGCTCCATCATCTGCTTTTTTTTTTCTTTTTTTTTTTATTTAAAAGGGTTGCAATTTCCAAGTGGTGCTTTTAGTGTTTGTACCATTTGCCAGCAAGTAACCAATATTAAATATTACAAAAGGCTTTTTCTTTGGATTGGACATCCACATTAGTTTGTATGTGGTATTTCCATCTTTTGGATAAAGCTTACATAACCAAATGTCTCACCCTCTCTATGCAGAAGTGTTGTGTTTTTAAAGTATTTAACGAGGATGGAGATTGAACCCTAACCTTTAGAGAGGAAGATCATGCCAATTACTAATAAGTTAACCTCATTTTGGCTTTAGGAGTCATTGATTAATGCTAAACCATTTTCTGTATTGGTCTCCTGCAGATCACTGAAGAGTGGACAACACAAGTTCAATCAAAATTTTGTAAGGATTCTTTTCAAGCTTTCTACAGAATTTTCTGATTCCAATTGCACTCTTCCTAGTAAAAACTCTAGTCACAACCACTGAACCATTGATTTCTTTTATTTGTGTTCTTTGTAACAATACATGGGGTGCGGCATGGGAAGTGGAGAATTAAACATAGTTACTTTAAGGTCAACACTACAAGCTATATGCCAATTGTATATTCTTAGTAAGAAGATGAAACGAGTCGTTTTAGTGTATGTTCGGATGAACTTTCTAAGTGTTGAAAAAATTGTTTATAAACATTTCCAAAGCCAATCCAATTGTACCCTTGATAAGATTAATAATATGAGGCCCTGTAAGCTTTCACCCGCCCTTCATAGGGTGTGCTATATATATGATTGTGCAAATTGTGTGTTGGTGTCAATGCAGCAGGGGAAGATGGCCAAGAGGACGCCGATTGCAATGATGTCAACATGTTGCTATTCTCGGCCGTGGTGCAGGCTTGATTTGTAGAGTTTCAGAAGCAAAATTAAGTTGCATGTATTCAGGTATTTCTCTACCTAATTTCATGATCTCGCCCTGCCCTCCCGTCTACTAAATCCCTTTTATCTTTATCTTTTGGGAAAAAAAAAAAAAAAAACTCTGATGAAAACTAGTAGTCTGTTAATACTTGTTTTTTTTCTTCATTTTCTACACATGATATGATCTCGTTAGTAACGTTTTTTACCCCACGAGGCCATATTCATTCAAATTTTCAACAGCCTCCTCAAAGCTACTTTTTGAACTCTTCCATTAACAAAAATATACGTAAGATTTTTTTTTTAAAGTAATAATTAAAAATCTGAAATAATAACATATAAAATTTTGAGTTGGCTCCTGCAGGTACCTTAATATCAGTTTGGCAACGGTAAAAGATGTCAAGAGGTTAAACGAAACAGGTCTGTGAGCCATATGTAATTTGAGTATGAACAATTTACTGAAAAGCACAGTTTTTACAAAAATTTGGAAACAACATATTTGCATTAAAAAAACATCAAAAGCAGCATTATTAAGCTAAAACAATTAAAATTATGTAACAAAGCAATTTTTT

mRNA sequence

GCGCGAAAACCCTAGCGGAGAGAGAGTATGGGGATCGATCATCATCTTGATCAACATCATCAAGCGGCCGATGGATTGATGAATTTGTTCACGAAGGCCAATCTCGATCTCTCAGTAGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATCTATCCTGAAAGCGCGAATCCAATGAAGTTAGTTTCTAGGATAAAGAAGATACAGGATGATATATCAACATTGAAAGAGCAGTGTCGTGAAGTTTTAATAGCTAAACAGGATGTAATCGATCAGGCCCGGACGGTTCTTCTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCATACCCTTTGTTAGTGATCTCGATGATCCTGCATTTTCGGAATTCAAGCAGATCACTGAAGAGTGGACAACACAAGTTCAATCAAAATTTTCAGGGGAAGATGGCCAAGAGGACGCCGATTGCAATGATGTCAACATGTTGCTATTCTCGGCCGTGGTGCAGGCTTGATTTGTAGAGTTTCAGAAGCAAAATTAAGTTGCATGTATTCAGGTACCTTAATATCAGTTTGGCAACGGTAAAAGATGTCAAGAGGTTAAACGAAACAGGTCTGTGAGCCATATGTAATTTGAGTATGAACAATTTACTGAAAAGCACAGTTTTTACAAAAATTTGGAAACAACATATTTGCATTAAAAAAACATCAAAAGCAGCATTATTAAGCTAAAACAATTAAAATTATGTAACAAAGCAATTTTTT

Coding sequence (CDS)

ATGGGGATCGATCATCATCTTGATCAACATCATCAAGCGGCCGATGGATTGATGAATTTGTTCACGAAGGCCAATCTCGATCTCTCAGTAGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATCTATCCTGAAAGCGCGAATCCAATGAAGTTAGTTTCTAGGATAAAGAAGATACAGGATGATATATCAACATTGAAAGAGCAGTGTCGTGAAGTTTTAATAGCTAAACAGGATGTAATCGATCAGGCCCGGACGGTTCTTCTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCATACCCTTTGTTAGTGATCTCGATGATCCTGCATTTTCGGAATTCAAGCAGATCACTGAAGAGTGGACAACACAAGTTCAATCAAAATTTTCAGGGGAAGATGGCCAAGAGGACGCCGATTGCAATGATGTCAACATGTTGCTATTCTCGGCCGTGGTGCAGGCTTGA

Protein sequence

MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQDDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA*
Homology
BLAST of CSPI04G26060 vs. ExPASy Swiss-Prot
Match: Q8WVK7 (Spindle and kinetochore-associated protein 2 OS=Homo sapiens OX=9606 GN=SKA2 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 2.3e-04
Identity = 28/88 (31.82%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 15  DGLMNLFTKANLDLSVVQYRLEKEFQQIYPESA---NPMKLVSRIKKIQDDISTLKEQCR 74
           D L  +F KA  DL  +QYRLE E +  +P+SA   NP+ L+  +  I+    TL  + +
Sbjct: 6   DKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTLYARFK 65

Query: 75  EVLIAKQDVIDQARTVLLGNRNMLQKMQ 100
            V + +++   +    +    NM+QK+Q
Sbjct: 66  PVAVEQKESKSRICATVKKTMNMIQKLQ 93

BLAST of CSPI04G26060 vs. ExPASy Swiss-Prot
Match: Q4R8E8 (Spindle and kinetochore-associated protein 2 OS=Macaca fascicularis OX=9541 GN=SKA2 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 6.8e-04
Identity = 32/119 (26.89%), Postives = 59/119 (49.58%), Query Frame = 0

Query: 15  DGLMNLFTKANLDLSVVQYRLEKEFQQIYPESA---NPMKLVSRIKKIQDDISTLKEQCR 74
           D L  +F KA  DL  +QYRLE E +  +P+SA   NP+ L+  +  ++    TL  + +
Sbjct: 6   DKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSALKSRYQTLYARFK 65

Query: 75  EVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLD-DPAFSEFKQITEEWTTQV 130
            V + +++   +    +    N++QK+Q        +DL+  P   E K   E++ + +
Sbjct: 66  PVAVEQKETKSRICATVNKTMNVIQKLQKQ------TDLELSPLTKEEKTAAEQFKSHM 118

BLAST of CSPI04G26060 vs. ExPASy TrEMBL
Match: A0A0A0L5I9 (Protein FAM33A OS=Cucumis sativus OX=3659 GN=Csa_4G651780 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.9e-81
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ
Sbjct: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ
Sbjct: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           ITEEWTTQVQSKFSGEDGQEDADCNDVN LLFSAVVQA
Sbjct: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNKLLFSAVVQA 158

BLAST of CSPI04G26060 vs. ExPASy TrEMBL
Match: A0A5A7VFB8 (Protein FAM33A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001430 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.8e-75
Identity = 148/158 (93.67%), Postives = 152/158 (96.20%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MGIDHHL QHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ
Sbjct: 1   MGIDHHLHQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVL AKQDVID+ARTVLLGNRNMLQKMQASMGIPFVSDLDDPA +EFKQ
Sbjct: 61  DDISTLKEQCREVLTAKQDVIDKARTVLLGNRNMLQKMQASMGIPFVSDLDDPALAEFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           ITEEWTTQVQSKF GED QEDADC++VN LLFSAVVQA
Sbjct: 121 ITEEWTTQVQSKF-GEDAQEDADCSNVNKLLFSAVVQA 157

BLAST of CSPI04G26060 vs. ExPASy TrEMBL
Match: A0A1S3BUQ8 (Protein FAM33A OS=Cucumis melo OX=3656 GN=LOC103493729 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.8e-75
Identity = 148/158 (93.67%), Postives = 152/158 (96.20%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MGIDHHL QHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ
Sbjct: 1   MGIDHHLHQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVL AKQDVID+ARTVLLGNRNMLQKMQASMGIPFVSDLDDPA +EFKQ
Sbjct: 61  DDISTLKEQCREVLTAKQDVIDKARTVLLGNRNMLQKMQASMGIPFVSDLDDPALAEFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           ITEEWTTQVQSKF GED QEDADC++VN LLFSAVVQA
Sbjct: 121 ITEEWTTQVQSKF-GEDAQEDADCSNVNKLLFSAVVQA 157

BLAST of CSPI04G26060 vs. ExPASy TrEMBL
Match: A0A6J1CJF1 (Protein FAM33A OS=Momordica charantia OX=3673 GN=LOC111011710 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.9e-66
Identity = 134/156 (85.90%), Postives = 146/156 (93.59%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MG+ HHL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYP+SANPMKLVSRIKKI+
Sbjct: 1   MGL-HHLHQHHQAADGLANLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLVSRIKKIR 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTL+EQCRE+L+AKQDVID+ARTVL+GNRNMLQKMQASMGIP VSDLDD AF++FK+
Sbjct: 61  DDISTLQEQCREILMAKQDVIDKARTVLVGNRNMLQKMQASMGIPLVSDLDDHAFAQFKK 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVV 157
           ITEEWTTQVQSK  G+DGQEDAD NDVN LLFSAVV
Sbjct: 121 ITEEWTTQVQSKI-GDDGQEDADSNDVNKLLFSAVV 154

BLAST of CSPI04G26060 vs. ExPASy TrEMBL
Match: A0A6J1F6V9 (Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.2e-64
Identity = 133/157 (84.71%), Postives = 143/157 (91.08%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MG+D HL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYP+SANPMKL  RIKKI+
Sbjct: 1   MGLD-HLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIR 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVL+AKQDVID+ARTVL+GNRNM+ KMQASMG PFVSD DDPAF +FKQ
Sbjct: 61  DDISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQ 158
           ITEEWTTQVQSK  GEDGQEDA+ +DVN LLFSAVVQ
Sbjct: 121 ITEEWTTQVQSKI-GEDGQEDAESSDVNKLLFSAVVQ 155

BLAST of CSPI04G26060 vs. NCBI nr
Match: XP_004145489.1 (spindle and kinetochore-associated protein 2 [Cucumis sativus] >KGN55411.1 hypothetical protein Csa_012758 [Cucumis sativus])

HSP 1 Score: 311.6 bits (797), Expect = 3.9e-81
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ
Sbjct: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ
Sbjct: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           ITEEWTTQVQSKFSGEDGQEDADCNDVN LLFSAVVQA
Sbjct: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNKLLFSAVVQA 158

BLAST of CSPI04G26060 vs. NCBI nr
Match: XP_008452819.1 (PREDICTED: spindle and kinetochore-associated protein 2 [Cucumis melo] >KAA0064565.1 spindle and kinetochore-associated protein 2 [Cucumis melo var. makuwa] >TYK20025.1 spindle and kinetochore-associated protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-74
Identity = 148/158 (93.67%), Postives = 152/158 (96.20%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MGIDHHL QHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ
Sbjct: 1   MGIDHHLHQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVL AKQDVID+ARTVLLGNRNMLQKMQASMGIPFVSDLDDPA +EFKQ
Sbjct: 61  DDISTLKEQCREVLTAKQDVIDKARTVLLGNRNMLQKMQASMGIPFVSDLDDPALAEFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           ITEEWTTQVQSKF GED QEDADC++VN LLFSAVVQA
Sbjct: 121 ITEEWTTQVQSKF-GEDAQEDADCSNVNKLLFSAVVQA 157

BLAST of CSPI04G26060 vs. NCBI nr
Match: XP_038898183.1 (spindle and kinetochore-associated protein 2 [Benincasa hispida] >XP_038898184.1 spindle and kinetochore-associated protein 2 [Benincasa hispida])

HSP 1 Score: 272.3 bits (695), Expect = 2.6e-69
Identity = 139/153 (90.85%), Postives = 146/153 (95.42%), Query Frame = 0

Query: 6   HLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQDDIST 65
           HL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYPESANPMKLVSRIKKI+DDIST
Sbjct: 5   HLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRDDIST 64

Query: 66  LKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQITEEW 125
           LKEQCREVL+AKQDVID+ARTVL+GNRNMLQK+QASMG+PFVSDLDDPAF+EFKQITEEW
Sbjct: 65  LKEQCREVLMAKQDVIDKARTVLVGNRNMLQKLQASMGVPFVSDLDDPAFAEFKQITEEW 124

Query: 126 TTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQA 159
           T QVQSKF GEDGQEDAD NDVN LLFSAVVQA
Sbjct: 125 TRQVQSKF-GEDGQEDADYNDVNKLLFSAVVQA 156

BLAST of CSPI04G26060 vs. NCBI nr
Match: XP_022141272.1 (spindle and kinetochore-associated protein 2 [Momordica charantia])

HSP 1 Score: 261.2 bits (666), Expect = 6.0e-66
Identity = 134/156 (85.90%), Postives = 146/156 (93.59%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MG+ HHL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYP+SANPMKLVSRIKKI+
Sbjct: 1   MGL-HHLHQHHQAADGLANLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLVSRIKKIR 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTL+EQCRE+L+AKQDVID+ARTVL+GNRNMLQKMQASMGIP VSDLDD AF++FK+
Sbjct: 61  DDISTLQEQCREILMAKQDVIDKARTVLVGNRNMLQKMQASMGIPLVSDLDDHAFAQFKK 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVV 157
           ITEEWTTQVQSK  G+DGQEDAD NDVN LLFSAVV
Sbjct: 121 ITEEWTTQVQSKI-GDDGQEDADSNDVNKLLFSAVV 154

BLAST of CSPI04G26060 vs. NCBI nr
Match: KAG6591515.1 (hypothetical protein SDJN03_13861, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 259.6 bits (662), Expect = 1.7e-65
Identity = 134/157 (85.35%), Postives = 144/157 (91.72%), Query Frame = 0

Query: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60
           MG+D HL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYP+SANPMKL  RIKKI+
Sbjct: 1   MGLD-HLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIR 60

Query: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120
           DDISTLKEQCREVL+AKQDVID+ARTVL+GNRNM+ KMQASMG PFVSD DDPAF +FKQ
Sbjct: 61  DDISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ 120

Query: 121 ITEEWTTQVQSKFSGEDGQEDADCNDVNMLLFSAVVQ 158
           ITEEWTTQVQSK  GEDGQEDA+C+DVN LLFSAVVQ
Sbjct: 121 ITEEWTTQVQSKI-GEDGQEDAECSDVNKLLFSAVVQ 155

BLAST of CSPI04G26060 vs. TAIR 10
Match: AT2G24970.1 (unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 28; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink). )

HSP 1 Score: 164.9 bits (416), Expect = 5.5e-41
Identity = 81/148 (54.73%), Postives = 114/148 (77.03%), Query Frame = 0

Query: 8   DQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQDDISTLK 67
           + HHQA D L+N+F++A+ DL+VV  +L+KEFQQ+YP +ANPMKL+ RIKK+Q+D++ LK
Sbjct: 4   NHHHQAVDNLLNVFSRASHDLTVVHSKLDKEFQQMYPANANPMKLIQRIKKLQEDVTLLK 63

Query: 68  EQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQITEEWTT 127
            QC ++L AKQD+ID+A+T L+GN N++QKM AS+G     D DD A ++F QI +EWT 
Sbjct: 64  HQCLDLLSAKQDLIDKAQTTLVGNCNLIQKMNASLGESTNGDTDD-ALADFNQIIDEWTM 123

Query: 128 QVQSKFSGEDGQEDADCNDVNMLLFSAV 156
           QV+S+  GE   EDAD  D+N +LFSA+
Sbjct: 124 QVRSRTVGE--TEDADKEDINKMLFSAI 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8WVK72.3e-0431.82Spindle and kinetochore-associated protein 2 OS=Homo sapiens OX=9606 GN=SKA2 PE=... [more]
Q4R8E86.8e-0426.89Spindle and kinetochore-associated protein 2 OS=Macaca fascicularis OX=9541 GN=S... [more]
Match NameE-valueIdentityDescription
A0A0A0L5I91.9e-8199.37Protein FAM33A OS=Cucumis sativus OX=3659 GN=Csa_4G651780 PE=3 SV=1[more]
A0A5A7VFB85.8e-7593.67Protein FAM33A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00143... [more]
A0A1S3BUQ85.8e-7593.67Protein FAM33A OS=Cucumis melo OX=3656 GN=LOC103493729 PE=3 SV=1[more]
A0A6J1CJF12.9e-6685.90Protein FAM33A OS=Momordica charantia OX=3673 GN=LOC111011710 PE=3 SV=1[more]
A0A6J1F6V91.2e-6484.71Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004145489.13.9e-8199.37spindle and kinetochore-associated protein 2 [Cucumis sativus] >KGN55411.1 hypot... [more]
XP_008452819.11.2e-7493.67PREDICTED: spindle and kinetochore-associated protein 2 [Cucumis melo] >KAA00645... [more]
XP_038898183.12.6e-6990.85spindle and kinetochore-associated protein 2 [Benincasa hispida] >XP_038898184.1... [more]
XP_022141272.16.0e-6685.90spindle and kinetochore-associated protein 2 [Momordica charantia][more]
KAG6591515.11.7e-6585.35hypothetical protein SDJN03_13861, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G24970.15.5e-4154.73unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bac... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042091Ska2, N-terminalPFAMPF16740SKA2coord: 11..118
e-value: 6.1E-40
score: 135.5
NoneNo IPR availableGENE3D6.10.250.1380coord: 11..124
e-value: 1.2E-25
score: 91.7
IPR026762Spindle and kinetochore-associated protein 2PANTHERPTHR32017SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2coord: 13..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G26060.1CSPI04G26060.1mRNA
CSPI04G26060.2CSPI04G26060.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0007059 chromosome segregation
biological_process GO:0000278 mitotic cell cycle
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000940 outer kinetochore
cellular_component GO:0005876 spindle microtubule
molecular_function GO:0008017 microtubule binding