CSPI04G26040 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G26040
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein TIC 55, chloroplastic
Location	Chr4: 23281685 .. 23282526 (+)
RNA-Seq Expression	CSPI04G26040
Synteny	CSPI04G26040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGCTCCGCCCATTTCTTTCCCTCTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTATCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGGGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTGTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGTAAAATAGGCGGAAAAAATAATGGGCCATGAAGGTTTCGAACCTGCGATCCGTTATTTGCTACGATATCATTTGATCTTTTAATTTTACCTTCTTATTTTCTCTCTCTTATTTTTTAATTCATCAAATACATCTTATTATATGTAATCTAATATCACTCATACAATTAAGCTAATCTGTGATACTTAGATGGTCTTAATTTAAAATGATTAAAATTTTGAATTATAGCTTAAAATTCAATGTAACTCTCTCCTATTTACATGGTGTGGTTTTAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGGTAATAGAGTCCGACCATGAGTACGTTTGGAATAACTTTTTAACTGTTGAAGAATAG

mRNA sequence

ATGGCTTCGCTCCGCCCATTTCTTTCCCTCTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTATCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGGGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTGTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGGTAATAGAGTCCGACCATGAGTACGTTTGGAATAACTTTTTAACTGTTGAAGAATAG

Coding sequence (CDS)

ATGGCTTCGCTCCGCCCATTTCTTTCCCTCTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTATCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGGGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTGTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGGTAATAGAGTCCGACCATGAGTACGTTTGGAATAACTTTTTAACTGTTGAAGAATAG

Protein sequence

MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIADPVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQVIESDHEYVWNNFLTVEE*

Homology

[Close]

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot
Match: Q9SK50 (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.8e-48
Identity = 100/171 (58.48%), Postives = 123/171 (71.93%), Query Frame = 0


Query: 6   PFLS----LSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRF-AVRTAAVAGAQIAD 65
           PFLS    L+P SP    T+ T   ++H+     S   + TK R   +R +AVAG  ++D
Sbjct: 4   PFLSSSLQLTPTSPILF-TKVTPTPIIHN---HRSTCTIPTKPRLRLLRRSAVAGTAVSD 63

Query: 66  PVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYR 125
             +    VLL P   ++ +    A Y+WTEEWYPLYLTK+VPEDAPLGLTV+D+Q+V+Y+
Sbjct: 64  QTEGGGDVLLNPEEEKRVE---VADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVLYK 123

Query: 126 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           DG G L C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 124 DGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 167

[Close]

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot
Match: O49931 (Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.4e-45
Identity = 88/161 (54.66%), Postives = 112/161 (69.57%), Query Frame = 0


Query: 16  APNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIADPV----DDNQVVLLG 75
           +P P++ T   L  +P  + SF    +  R        A A + D      +++Q VL+G
Sbjct: 24  SPPPSKKT--LLCTNPSSNFSFNKALSSRRRKQAWCVAAAADVKDATLLDGEEDQKVLVG 83

Query: 76  PTT-SEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRDGAGELHCHE 135
           P++  E+  +   A Y+WTEEWYPLYLTK+VP DAPLGL V+DK +V++RDG  +  C+E
Sbjct: 84  PSSEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLGLKVYDKNIVLFRDGNDQFQCYE 143

Query: 136 DRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           DRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 144 DRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 182

[Close]

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot
Match: Q0DV66 (Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PAO PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 58/172 (33.72%), Postives = 86/172 (50.00%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           M  + P  SL  +SP P    P +  +   P LS+S +L   + R   R    A   +  
Sbjct: 1   MPVMAPTASLL-LSPRP---LPASRRVPSLPALSASGRLRLRRARADTRLRVAAPPSVPG 60

Query: 61  PVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYR 120
             D        P  +E     A   + W + WYP+ L +D+    P    + ++ LV+++
Sbjct: 61  EADQ------APGETEPSTSSADEKFVWRDHWYPVSLVEDLDPSVPTPFQLLNRDLVIWK 120

Query: 121 D-GAGELHCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQ 171
           D  +GE    +DRCPHRLA LSEG++ + G L+C YHGW F+G G C +IPQ
Sbjct: 121 DPKSGEWVALDDRCPHRLAPLSEGRIDETGCLQCSYHGWSFDGSGACTRIPQ 162

[Close]

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot
Match: Q9FYC2 (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-16
Identity = 55/176 (31.25%), Postives = 80/176 (45.45%), Query Frame = 0


Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PFLS +   P      P+   L H+PL                R AA      +D  ++ 
Sbjct: 22  PFLSPTTKFPLKVSISPSRSKLFHNPL----------------RVAAPPSVPTSDSTEEK 81

Query: 66  QVVLLGPTTSEKPDDEATAG--YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRD-G 125
           ++        E   D+   G  + W + WYP+ L +D+  + P    +  + LV++ D  
Sbjct: 82  RI------EEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRDLVLWFDRN 141

Query: 126 AGELHCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQVIESDHE 178
             +    +D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ   S  E
Sbjct: 142 DQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPE 175

[Close]

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot
Match: Q9ZWM5 (Chlorophyllide a oxygenase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=CAO PE=2 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 30/78 (38.46%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 92  WYPLYLTKDVPEDAPLGLTVFDKQLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLE 151
           WYP   +  +P+D  +   +F +  V++RD  G+  C  D C HR   LS G++++G++ 
Sbjct: 305 WYPAEFSARLPKDTLVPFELFGEPWVMFRDEKGQPSCIRDECAHRGCPLSLGKVVEGQVM 364

Query: 152 CLYHGWQFEGEGKCVKIP 170
           C YHGW+F G+G C K+P
Sbjct: 365 CPYHGWEFNGDGACTKMP 382

[Close]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL
Match: A0A0A0L0A9 (Rieske domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G651770 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.5e-93
Identity = 167/171 (97.66%), Postives = 169/171 (98.83%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPL SSSFKLVATKTRFAVRTAAVAGAQIAD
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  PVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYR 120
           PVDDNQVVLLGPTTSEKPDDEATA YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLV+YR
Sbjct: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120

Query: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 171

[Close]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL
Match: A0A5D3D8Y6 (Protein TIC 55 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001440 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.5e-81
Identity = 152/176 (86.36%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPV PAPN TRPT+  L+H+P  SSSFKLV+ K RFAVRTAAVAGA+IAD
Sbjct: 1   MASLRPFLSLSPVFPAPNHTRPTDSLLIHAP-FSSSFKLVSAKPRFAVRTAAVAGAEIAD 60

Query: 61  PV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120
           PV     DDNQVVLL PTTSEKPD EATA YNWTEEWYPLYLT+DVPEDAPLGLTVFDKQ
Sbjct: 61  PVGDLKDDDNQVVLLDPTTSEKPDGEATAEYNWTEEWYPLYLTRDVPEDAPLGLTVFDKQ 120

Query: 121 LVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           LV+YRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 175

[Close]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL
Match: A0A1S3BUS9 (protein TIC 55, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493728 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.5e-81
Identity = 152/176 (86.36%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPV PAPN TRPT+  L+H+P  SSSFKLV+ K RFAVRTAAVAGA+IAD
Sbjct: 1   MASLRPFLSLSPVFPAPNHTRPTDSLLIHAP-FSSSFKLVSAKPRFAVRTAAVAGAEIAD 60

Query: 61  PV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120
           PV     DDNQVVLL PTTSEKPD EATA YNWTEEWYPLYLT+DVPEDAPLGLTVFDKQ
Sbjct: 61  PVGDLKDDDNQVVLLDPTTSEKPDGEATAEYNWTEEWYPLYLTRDVPEDAPLGLTVFDKQ 120

Query: 121 LVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           LV+YRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 175

[Close]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL
Match: A0A6J1IH83 (protein TIC 55, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477196 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.5e-61
Identity = 124/177 (70.06%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSS-SFKLVATKTRFAVRTAAVAGAQIA 60
           MASLRPFLS+SP S AP  T   N     SP  SS  FKLVA + RF VR  AV  A  A
Sbjct: 1   MASLRPFLSISPFSRAPKRTHFANVLNAPSPSASSLPFKLVAARKRFVVRGDAVVDAGTA 60

Query: 61  DPV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDK 120
             +     DDNQ V+LGPTT E+    +T  Y+WTEEWYPLYLT+DVPEDAPLGLTVFDK
Sbjct: 61  ASIGDLKDDDNQAVILGPTTDEELSGGSTVEYDWTEEWYPLYLTRDVPEDAPLGLTVFDK 120

Query: 121 QLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           QLV+YRDG+GEL CHEDRCPHRLAKLSEGQLIDG+LECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 QLVLYRDGSGELRCHEDRCPHRLAKLSEGQLIDGKLECLYHGWQFEGEGKCVKIPQL 177

[Close]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL
Match: A0A6J1FAV6 (protein TIC 55, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443647 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.8e-60
Identity = 122/177 (68.93%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSS-SFKLVATKTRFAVRTAAVAGAQIA 60
           MASLRPFLS+SP S AP  TR  N     SP  SS  FKLVA + RF VR  AV  A   
Sbjct: 1   MASLRPFLSVSPFSHAPKRTRFANVLNAPSPSASSLPFKLVAARKRFVVRGDAVVDAGTT 60

Query: 61  DPV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDK 120
             +     DD+Q V+LGP T E+    +T  Y+WTEEWYPLYLT+DVPEDAPLGLTVFDK
Sbjct: 61  ASIGDLKDDDDQAVILGPATDEELSGGSTVEYDWTEEWYPLYLTRDVPEDAPLGLTVFDK 120

Query: 121 QLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           QLV+YRDG GEL CHEDRCPHRLAKLSEGQLIDG+LECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 QLVLYRDGTGELRCHEDRCPHRLAKLSEGQLIDGKLECLYHGWQFEGEGKCVKIPQL 177

[Close]

BLAST of CSPI04G26040 vs. NCBI nr
Match: XP_004145578.1 (protein TIC 55, chloroplastic [Cucumis sativus] >KGN55410.1 hypothetical protein Csa_012107 [Cucumis sativus])

HSP 1 Score: 352.1 bits (902), Expect = 3.1e-93
Identity = 167/171 (97.66%), Postives = 169/171 (98.83%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPL SSSFKLVATKTRFAVRTAAVAGAQIAD
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  PVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYR 120
           PVDDNQVVLLGPTTSEKPDDEATA YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLV+YR
Sbjct: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120

Query: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 171

[Close]

BLAST of CSPI04G26040 vs. NCBI nr
Match: XP_008452818.2 (PREDICTED: protein TIC 55, chloroplastic [Cucumis melo] >KAA0064564.1 protein TIC 55 [Cucumis melo var. makuwa] >TYK20026.1 protein TIC 55 [Cucumis melo var. makuwa])

HSP 1 Score: 309.7 bits (792), Expect = 1.8e-80
Identity = 152/176 (86.36%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPV PAPN TRPT+  L+H+P  SSSFKLV+ K RFAVRTAAVAGA+IAD
Sbjct: 1   MASLRPFLSLSPVFPAPNHTRPTDSLLIHAP-FSSSFKLVSAKPRFAVRTAAVAGAEIAD 60

Query: 61  PV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120
           PV     DDNQVVLL PTTSEKPD EATA YNWTEEWYPLYLT+DVPEDAPLGLTVFDKQ
Sbjct: 61  PVGDLKDDDNQVVLLDPTTSEKPDGEATAEYNWTEEWYPLYLTRDVPEDAPLGLTVFDKQ 120

Query: 121 LVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           LV+YRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 175

[Close]

BLAST of CSPI04G26040 vs. NCBI nr
Match: XP_038897660.1 (protein TIC 55, chloroplastic [Benincasa hispida])

HSP 1 Score: 267.3 bits (682), Expect = 1.0e-67
Identity = 135/176 (76.70%), Postives = 144/176 (81.82%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           M SLRPFLSLS  S A NPTRPTNF LL + L SSSFK VATK   +VRT AVA A+ A 
Sbjct: 1   MVSLRPFLSLSSFSHARNPTRPTNFLLLRA-LSSSSFKSVATKKSSSVRTGAVADAEAAS 60

Query: 61  PV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120
           P      DD QVVLLGP T E+P  EATA Y WTEEWYPLYLT++VPEDAPLGLTVFDKQ
Sbjct: 61  PSGDLKDDDAQVVLLGPATVEEPGGEATAEYEWTEEWYPLYLTRNVPEDAPLGLTVFDKQ 120

Query: 121 LVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           LV+YRDG GEL CHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEG+GKCVKIPQ+
Sbjct: 121 LVLYRDGTGELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGKGKCVKIPQL 175

[Close]

BLAST of CSPI04G26040 vs. NCBI nr
Match: XP_022976996.1 (protein TIC 55, chloroplastic isoform X1 [Cucurbita maxima] >XP_022976997.1 protein TIC 55, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 245.7 bits (626), Expect = 3.1e-61
Identity = 124/177 (70.06%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSS-SFKLVATKTRFAVRTAAVAGAQIA 60
           MASLRPFLS+SP S AP  T   N     SP  SS  FKLVA + RF VR  AV  A  A
Sbjct: 1   MASLRPFLSISPFSRAPKRTHFANVLNAPSPSASSLPFKLVAARKRFVVRGDAVVDAGTA 60

Query: 61  DPV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDK 120
             +     DDNQ V+LGPTT E+    +T  Y+WTEEWYPLYLT+DVPEDAPLGLTVFDK
Sbjct: 61  ASIGDLKDDDNQAVILGPTTDEELSGGSTVEYDWTEEWYPLYLTRDVPEDAPLGLTVFDK 120

Query: 121 QLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           QLV+YRDG+GEL CHEDRCPHRLAKLSEGQLIDG+LECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 QLVLYRDGSGELRCHEDRCPHRLAKLSEGQLIDGKLECLYHGWQFEGEGKCVKIPQL 177

[Close]

BLAST of CSPI04G26040 vs. NCBI nr
Match: XP_022937322.1 (protein TIC 55, chloroplastic isoform X1 [Cucurbita moschata] >XP_022937323.1 protein TIC 55, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 241.5 bits (615), Expect = 5.9e-60
Identity = 122/177 (68.93%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLLSS-SFKLVATKTRFAVRTAAVAGAQIA 60
           MASLRPFLS+SP S AP  TR  N     SP  SS  FKLVA + RF VR  AV  A   
Sbjct: 1   MASLRPFLSVSPFSHAPKRTRFANVLNAPSPSASSLPFKLVAARKRFVVRGDAVVDAGTT 60

Query: 61  DPV-----DDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDK 120
             +     DD+Q V+LGP T E+    +T  Y+WTEEWYPLYLT+DVPEDAPLGLTVFDK
Sbjct: 61  ASIGDLKDDDDQAVILGPATDEELSGGSTVEYDWTEEWYPLYLTRDVPEDAPLGLTVFDK 120

Query: 121 QLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           QLV+YRDG GEL CHEDRCPHRLAKLSEGQLIDG+LECLYHGWQFEGEGKCVKIPQ+
Sbjct: 121 QLVLYRDGTGELRCHEDRCPHRLAKLSEGQLIDGKLECLYHGWQFEGEGKCVKIPQL 177

[Close]

BLAST of CSPI04G26040 vs. TAIR 10
Match: AT2G24820.1 (translocon at the inner envelope membrane of chloroplasts 55-II )

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-49
Identity = 100/171 (58.48%), Postives = 123/171 (71.93%), Query Frame = 0


Query: 6   PFLS----LSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRF-AVRTAAVAGAQIAD 65
           PFLS    L+P SP    T+ T   ++H+     S   + TK R   +R +AVAG  ++D
Sbjct: 4   PFLSSSLQLTPTSPILF-TKVTPTPIIHN---HRSTCTIPTKPRLRLLRRSAVAGTAVSD 63

Query: 66  PVDDNQVVLLGPTTSEKPDDEATAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYR 125
             +    VLL P   ++ +    A Y+WTEEWYPLYLTK+VPEDAPLGLTV+D+Q+V+Y+
Sbjct: 64  QTEGGGDVLLNPEEEKRVE---VADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVLYK 123

Query: 126 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQV 172
           DG G L C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ+
Sbjct: 124 DGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQL 167

[Close]

BLAST of CSPI04G26040 vs. TAIR 10
Match: AT3G44880.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 87.4 bits (215), Expect = 1.3e-17
Identity = 55/176 (31.25%), Postives = 80/176 (45.45%), Query Frame = 0


Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLLSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PFLS +   P      P+   L H+PL                R AA      +D  ++ 
Sbjct: 22  PFLSPTTKFPLKVSISPSRSKLFHNPL----------------RVAAPPSVPTSDSTEEK 81

Query: 66  QVVLLGPTTSEKPDDEATAG--YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRD-G 125
           ++        E   D+   G  + W + WYP+ L +D+  + P    +  + LV++ D  
Sbjct: 82  RI------EEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRDLVLWFDRN 141

Query: 126 AGELHCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQVIESDHE 178
             +    +D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ   S  E
Sbjct: 142 DQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPE 175

[Close]

BLAST of CSPI04G26040 vs. TAIR 10
Match: AT4G25650.1 (ACD1-like )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = 0


Query: 73  TTSEKPDDEA-----TAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRD-GAGEL 132
           +T+  P+ EA     +  ++W   WYP+    D+ +  P G  V    LVV+ D    + 
Sbjct: 61  STNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQW 120

Query: 133 HCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQ 171
              +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ
Sbjct: 121 KVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQ 165

[Close]

BLAST of CSPI04G26040 vs. TAIR 10
Match: AT4G25650.2 (ACD1-like )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = 0


Query: 73  TTSEKPDDEA-----TAGYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVVYRD-GAGEL 132
           +T+  P+ EA     +  ++W   WYP+    D+ +  P G  V    LVV+ D    + 
Sbjct: 61  STNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQW 120

Query: 133 HCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQ 171
              +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ
Sbjct: 121 KVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQ 165

[Close]

BLAST of CSPI04G26040 vs. TAIR 10
Match: AT1G44446.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 75.1 bits (183), Expect = 6.8e-14
Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 92  WYPLYLTKDVPEDAPLGLTVFDKQLVVYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLE 151
           WYP+  T D+  D  + +  F++  V++R   G+  C  + C HR   L  G + +GR++
Sbjct: 221 WYPVAFTADLKHDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQ 280

Query: 152 CLYHGWQFEGEGKCVKIP 170
           C YHGW++  +G+C K+P
Sbjct: 281 CPYHGWEYSTDGECKKMP 298

The following BLAST results are available for this feature:

BLAST of CSPI04G26040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SK50	1.8e-48	58.48	Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1	[more]
O49931	9.4e-45	54.66	Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1	[more]
Q0DV66	1.8e-19	33.72	Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9FYC2	1.9e-16	31.25	Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO P...	[more]
Q9ZWM5	1.3e-14	38.46	Chlorophyllide a oxygenase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 G...	[more]

BLAST of CSPI04G26040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0A9	1.5e-93	97.66	Rieske domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G651770 PE=4...	[more]
A0A5D3D8Y6	8.5e-81	86.36	Protein TIC 55 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00144...	[more]
A0A1S3BUS9	8.5e-81	86.36	protein TIC 55, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493728 PE=4 SV=1	[more]
A0A6J1IH83	1.5e-61	70.06	protein TIC 55, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1FAV6	2.8e-60	68.93	protein TIC 55, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of CSPI04G26040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145578.1	3.1e-93	97.66	protein TIC 55, chloroplastic [Cucumis sativus] >KGN55410.1 hypothetical protein...	[more]
XP_008452818.2	1.8e-80	86.36	PREDICTED: protein TIC 55, chloroplastic [Cucumis melo] >KAA0064564.1 protein TI...	[more]
XP_038897660.1	1.0e-67	76.70	protein TIC 55, chloroplastic [Benincasa hispida]	[more]
XP_022976996.1	3.1e-61	70.06	protein TIC 55, chloroplastic isoform X1 [Cucurbita maxima] >XP_022976997.1 prot...	[more]
XP_022937322.1	5.9e-60	68.93	protein TIC 55, chloroplastic isoform X1 [Cucurbita moschata] >XP_022937323.1 pr...	[more]

BLAST of CSPI04G26040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24820.1	1.3e-49	58.48	translocon at the inner envelope membrane of chloroplasts 55-II	[more]
AT3G44880.1	1.3e-17	31.25	Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain	[more]
AT4G25650.1	1.2e-15	39.05	ACD1-like	[more]
AT4G25650.2	1.2e-15	39.05	ACD1-like	[more]
AT1G44446.1	6.8e-14	34.62	Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	PFAM	PF00355	Rieske	coord: 91..171 e-value: 5.2E-22 score: 77.6
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	PROSITE	PS51296	RIESKE	coord: 92..186 score: 29.14065
IPR036922	Rieske [2Fe-2S] iron-sulphur domain superfamily	GENE3D	2.102.10.10		coord: 87..188 e-value: 8.9E-32 score: 111.5
IPR036922	Rieske [2Fe-2S] iron-sulphur domain superfamily	SUPERFAMILY	50022	ISP domain	coord: 85..173
None	No IPR available	PANTHER	PTHR21266	IRON-SULFUR DOMAIN CONTAINING PROTEIN	coord: 57..174
None	No IPR available	PANTHER	PTHR21266:SF29	PROTEIN TIC 55, CHLOROPLASTIC	coord: 57..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G26040.1	CSPI04G26040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045036	protein targeting to chloroplast
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031967	organelle envelope
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0010277	chlorophyllide a oxygenase [overall] activity
molecular_function	GO:0046872	metal ion binding