CSPI04G25680 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G25680
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMechanosensitive ion channel protein
LocationChr4: 22978697 .. 22982167 (+)
RNA-Seq ExpressionCSPI04G25680
SyntenyCSPI04G25680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACAAAACAAATATGGTCTATAAATCTTTGGCCCAGATGGGTTTTTTGTTAACCTTTTTCGCCATTTCTGTGTTCCAAGTGCCCCTAGTGTTGAGTGAAGCTCATTGTTCAACGAAGCTTTGACTACACTCCCTCTGTTCCCCATTGCACTCTACTTTTTGACATTTCTGGTTTGTTATTACGCCATTTTTCTTTTTCCCACCTTCAATCTCTTTGTTTTTGTTTTAACCAAGTGGGTTTTCACTCGTTTTTTCTGCTTCTTTGTGTGATTTTGCATTCAAGAAACTCTCTTTGTTTATATTTATCTTTGACATTGTGGGTTTGCTGAGGTTTTCTTTATTAAGCTTGATGATTTTGATTGTGTTTTGGGTTGCTCTCAACGACCCTTTTCTTTGATTTCGTTTGTTTAGTTCCAAGCTTGGATTGTGATGGATGTGAGCGGAAATAAAGGTATTCGAAGAGGTTCGTCTCAGAAGGAGAGTGGAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTGCTCTGTTCCGAAGCAAAACAGAGCCGATTCACAAACCAAAGAGCCGACCGGTTCAAGCGTTGGCTATGACAACGCTTCACCACTCGCTCCCACTCCGAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCCAGAAGATCTCTGAGAAGATCAACTCTTTCCAAACCCAAATCCAGATTTGGGGAACAGTCATGTTTTATCGATTCAGACATGCTTGAGGAGGAGAATCATGTGTCGTTGAGAGAACAAATCGGTGCAACTTCATCCAGAAGCTCAAATCTCAACACGCCGAAGGCCCAACCTGAGGGAGAAGATGATGGGAATATGGGGTCGAATGCAAAGCACAAGAAAGTGAAGGTGAAGACAGTGATTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGCTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAGTACTTTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTTGATCGAAGAAACCATATGATTTCTAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCTTTCTTATAGGGGCAGTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCTCTTTTCCTTCATCACGTTCTACAAACCCTCTTGAGGCCTCCTCTAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAACCAGACCGTAAAAAGGTGATTGACATAGGAAAGATTCACGAGCTGAAGCGAGAAAAGGTTTCAGCTTGGACAATGAAGGTACTGGTTGATGCAGTTACTAGTTCAGAGATGTCGGTCTCACAAATACTTGACGATGAAAGCTACAGAGATGTCGCCGATGGTGATATCACAAATGAGATGAAAGTTGCCAAAGAAGCCGCGAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTGAGAAACTTTCCTTTTTGCTTGTGATTTTTTGTTCAAGCTCATGTTAATATTTTACCATTTCCCCTTTGTTAGGTTCATAGAGGAAAGAGATCTTCTGGAATTCATGATTCCAGAAGAAGTTAACCTTGTGTGGCCACACTTTGAGGTAGATAAGACAAGGAAGATTGACATGAAAGCTCTAACAAATTGGGTGGTGAGACCTTGTATCTGTTATTTATCTTTATTCATATTGAGTCATCTAACAACTTGAACGCATTAAAATTGTCATTCTTTTATTAGACAACAATGAGAATTGTTTCTCGATTGCTCATCCCCTAATAAGCAAACAAAGTCTTAGAATACAATTAAATACATAACATAGGCTTCTTATGTTCGAGATGATTTAGAATGCTTAATTGGTTATGGCCAAACATTTAGTTTCTCTTACTTCCTTGTCTTCATTAATTTTCTAAAACATGTTTCAAGAACCAAAGCGTGTTCATAATGTTCTATAGCACGTAGTGATTTCGTTTCGTACAGGTGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCTTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAGTAGTAACAGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACAACCAAAGTACTCGTCTTCCTTCTAACTCAGCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACAGATGTGTTGTTGATGGTGTCCAGGTAAGCTTCTTAACTTACCATCATCAAGGTCTCCTGTTTCTTTCTGTCTTTAACTTGTGGCAATCTGCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAGCTCAACAATGAGAAGGTGTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCGTCGAATTCTCAATCGGTTTCGAAACACCAGTGGAGAGGATTGGGGCCATGAAAGAACAAATAAAGAGGTGAGTGGAAGTGATACAAATAATGAAGATTTTGAATGCAACTTTACAAATATTTTGATCATTCTGAAACAAAGAAAAACCATTGTTTCACAATATATGATTTGATCCTTATCAATGGCTGCAGGTATTTGGAGGAGAATCCACAACACTGGTACCCAAATCATAATGTGGTGGTGAAGGAGATCGAAAACGTAAATAAGATCAAGATTGCTCTTTATACAAATCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCAAGCTCGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGAAAGTCTACACAAAATCCTAAGCTTATGCTTCTGGATACTTTAACAAAGACATGGTTAGCTTTTTTAGCTGCTTGGTTAACTTAATTAGAGACACAAGTAACATTTAGTAGAAAGAATCCTATATCAGTATTAGTTTAGGCAAACTAGTTACTCACGGTGATTAATGGGGAAAACATGAATTTATGATTGACATTAGAATTGGAAGATATAGGTTGTAGAAGTGTATTAGTAGGTTAAGGTACTAACCTTATTTAGCTATGTAATATTATTTAGGATTGGTG

mRNA sequence

CAACAAAACAAATATGGTCTATAAATCTTTGGCCCAGATGGGTTTTTTGTTAACCTTTTTCGCCATTTCTGTGTTCCAAGTGCCCCTAGTGTTGAGTGAAGCTCATTGTTCAACGAAGCTTTGACTACACTCCCTCTGTTCCCCATTGCACTCTACTTTTTGACATTTCTGTTCCAAGCTTGGATTGTGATGGATGTGAGCGGAAATAAAGGTATTCGAAGAGGTTCGTCTCAGAAGGAGAGTGGAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTGCTCTGTTCCGAAGCAAAACAGAGCCGATTCACAAACCAAAGAGCCGACCGGTTCAAGCGTTGGCTATGACAACGCTTCACCACTCGCTCCCACTCCGAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCCAGAAGATCTCTGAGAAGATCAACTCTTTCCAAACCCAAATCCAGATTTGGGGAACAGTCATGTTTTATCGATTCAGACATGCTTGAGGAGGAGAATCATGTGTCGTTGAGAGAACAAATCGGTGCAACTTCATCCAGAAGCTCAAATCTCAACACGCCGAAGGCCCAACCTGAGGGAGAAGATGATGGGAATATGGGGTCGAATGCAAAGCACAAGAAAGTGAAGGTGAAGACAGTGATTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGCTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAGTACTTTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTTGATCGAAGAAACCATATGATTTCTAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCTTTCTTATAGGGGCAGTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCTCTTTTCCTTCATCACGTTCTACAAACCCTCTTGAGGCCTCCTCTAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAACCAGACCGTAAAAAGGTGATTGACATAGGAAAGATTCACGAGCTGAAGCGAGAAAAGGTTTCAGCTTGGACAATGAAGGTACTGGTTGATGCAGTTACTAGTTCAGAGATGTCGGTCTCACAAATACTTGACGATGAAAGCTACAGAGATGTCGCCGATGGTGATATCACAAATGAGATGAAAGTTGCCAAAGAAGCCGCGAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTTCATAGAGGAAAGAGATCTTCTGGAATTCATGATTCCAGAAGAAGTTAACCTTGTGTGGCCACACTTTGAGGTAGATAAGACAAGGAAGATTGACATGAAAGCTCTAACAAATTGGGTGGTGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCTTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAGTAGTAACAGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACAACCAAAGTACTCGTCTTCCTTCTAACTCAGCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACAGATGTGTTGTTGATGGTGTCCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAGCTCAACAATGAGAAGGTGTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCGTCGAATTCTCAATCGGTTTCGAAACACCAGTGGAGAGGATTGGGGCCATGAAAGAACAAATAAAGAGGTATTTGGAGGAGAATCCACAACACTGGTACCCAAATCATAATGTGGTGGTGAAGGAGATCGAAAACGTAAATAAGATCAAGATTGCTCTTTATACAAATCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCAAGCTCGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGAAAGTCTACACAAAATCCTAAGCTTATGCTTCTGGATACTTTAACAAAGACATGGTTAGCTTTTTTAGCTGCTTGGTTAACTTAATTAGAGACACAAGTAACATTTAGTAGAAAGAATCCTATATCAGTATTAGTTTAGGCAAACTAGTTACTCACGGTGATTAATGGGGAAAACATGAATTTATGATTGACATTAGAATTGGAAGATATAGGTTGTAGAAGTGTATTAGTAGGTTAAGGTACTAACCTTATTTAGCTATGTAATATTATTTAGGATTGGTG

Coding sequence (CDS)

ATGGATGTGAGCGGAAATAAAGGTATTCGAAGAGGTTCGTCTCAGAAGGAGAGTGGAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTGCTCTGTTCCGAAGCAAAACAGAGCCGATTCACAAACCAAAGAGCCGACCGGTTCAAGCGTTGGCTATGACAACGCTTCACCACTCGCTCCCACTCCGAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCCAGAAGATCTCTGAGAAGATCAACTCTTTCCAAACCCAAATCCAGATTTGGGGAACAGTCATGTTTTATCGATTCAGACATGCTTGAGGAGGAGAATCATGTGTCGTTGAGAGAACAAATCGGTGCAACTTCATCCAGAAGCTCAAATCTCAACACGCCGAAGGCCCAACCTGAGGGAGAAGATGATGGGAATATGGGGTCGAATGCAAAGCACAAGAAAGTGAAGGTGAAGACAGTGATTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGCTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAGTACTTTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTTGATCGAAGAAACCATATGATTTCTAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCTTTCTTATAGGGGCAGTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCTCTTTTCCTTCATCACGTTCTACAAACCCTCTTGAGGCCTCCTCTAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAACCAGACCGTAAAAAGGTGATTGACATAGGAAAGATTCACGAGCTGAAGCGAGAAAAGGTTTCAGCTTGGACAATGAAGGTACTGGTTGATGCAGTTACTAGTTCAGAGATGTCGGTCTCACAAATACTTGACGATGAAAGCTACAGAGATGTCGCCGATGGTGATATCACAAATGAGATGAAAGTTGCCAAAGAAGCCGCGAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTTCATAGAGGAAAGAGATCTTCTGGAATTCATGATTCCAGAAGAAGTTAACCTTGTGTGGCCACACTTTGAGGTAGATAAGACAAGGAAGATTGACATGAAAGCTCTAACAAATTGGGTGGTGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCTTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAGTAGTAACAGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACAACCAAAGTACTCGTCTTCCTTCTAACTCAGCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACAGATGTGTTGTTGATGGTGTCCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAGCTCAACAATGAGAAGGTGTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCGTCGAATTCTCAATCGGTTTCGAAACACCAGTGGAGAGGATTGGGGCCATGAAAGAACAAATAAAGAGGTATTTGGAGGAGAATCCACAACACTGGTACCCAAATCATAATGTGGTGGTGAAGGAGATCGAAAACGTAAATAAGATCAAGATTGCTCTTTATACAAATCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCAAGCTCGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGA

Protein sequence

MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEGH*
Homology
BLAST of CSPI04G25680 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 7.0e-189
Identity = 385/740 (52.03%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
           S    G GG+VV+ +  VE      K+  +    K    S       S L  +PNKPP+ 
Sbjct: 6   SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65

Query: 74  PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
           P  N   LT R+S  RS  SKPKSRF + SC +D+ +LEEE    +REQ+GA  S S   
Sbjct: 66  PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125

Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
              +A P  + + ++GS A                 +KKVK+            +I+   
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185

Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
              I+S LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245

Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
           L++KVLYFVHGLKKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S 
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305

Query: 314 LIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
           L GA  WL+KTLLLKILA+ F++N FFDRIQ+S+F  +VLQTL   PL+E+      E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365

Query: 374 AKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
                     +      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S +S  LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425

Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
            +Y   ++ AD +IT+EM+ A  AA  +F+NVA P   +IEE DLL FMI EEV+LV+P 
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485

Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
           F+   +T +I  KA T WVVKVY  R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545

Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
           LL+E+ATTKVL+F  TQL   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605

Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
           EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I  +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665

Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMEL 716
           E+I  YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+  E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725

BLAST of CSPI04G25680 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 2.7e-172
Identity = 355/748 (47.46%), Postives = 487/748 (65.11%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPL----APTP-- 73
           +++   NG EVV+ +S  E +   P+ + + +    P  S  G + + P+     PTP  
Sbjct: 2   AERRVSNGEEVVINVSDKE-DSKDPRASPSFNPLASP-DSDAGIEKSKPVPPISIPTPEI 61

Query: 74  -------NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 133
                  +KPPKIP   G L  R+SL RS  SKPKSRFGEQ  F      EE    SLRE
Sbjct: 62  YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121

Query: 134 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAK--------------HKKVKVKTV----- 193
           Q GA S    + +  +A P  + + ++ S A               +KKVK+  V     
Sbjct: 122 QFGAGSFARGSFD--RASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGM 181

Query: 194 -----IKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNV 253
                ++ +    I+  L+ SLT++ +    +WGL+ WKWC+L  V L G++ T W M+ 
Sbjct: 182 KPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 241

Query: 254 VVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKIL 313
           VVF+IE+N+LL+KKVLYFVHGLKK+VQV +W SLVL  W  LFD     +  +R T + L
Sbjct: 242 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFL 301

Query: 314 DAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVE 373
           D +TWT+ S L+G++L+L+KT  LK+LASKF++  FF+RIQES+F  +VLQTL  PPL+E
Sbjct: 302 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 361

Query: 374 D-------ESTAKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAV-TS 433
           +        ST      R   +  K   KKVID+GK+H +K+EKVSAWTM+VL++AV TS
Sbjct: 362 EAENVGRVPSTGHLSFTR--TKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTS 421

Query: 434 SEMSVSQILDD-ESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIP 493
              ++S  LD+  + ++  D +ITNEM+ A  AA ++F NVA P + +IEE DLL FMI 
Sbjct: 422 GISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIK 481

Query: 494 EEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALII 553
           EEV+LV P  E   T KI  K  T WVV VY  RKT+ H+L DTKTAVKQL+ LIT ++ 
Sbjct: 482 EEVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 541

Query: 554 VVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCV 613
           V+T ++W++L++IA+TK+L+   +Q    AFM G+TCK  FE+ +FVFVMHP+DVGDRCV
Sbjct: 542 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 601

Query: 614 VDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETP 673
           VDGV LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD V+F I F TP
Sbjct: 602 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 661

Query: 674 VERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRR 716
            E+IG +K +I  YL  N QHWYP   V+V+ IEN+NK+ + +   HT+NFQ + EK+ R
Sbjct: 662 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 721

BLAST of CSPI04G25680 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 9.9e-127
Identity = 259/625 (41.44%), Postives = 382/625 (61.12%), Query Frame = 0

Query: 134 TPKAQPEGEDDG-----NMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFL 193
           +PK Q E E+D      ++    +  K+ +  V++W+ +  II+  V +L +  L+ + L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270

Query: 194 WGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWL 253
           W L++WKW  +  V++CG + + W++ +VVF IERNFLL+K+VLYFV+G++K+VQ  LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330

Query: 254 SLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILASKFH 313
            LVL  W  LFD +     +     K L  VT      L+G +LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEK----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390

Query: 314 MNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKK-------------- 373
           M+ +FDRIQESLF  +V++TL  PPL+E +   +    R   E KK              
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE-ERISVEVKKFQNPGGVEIQSGAQ 450

Query: 374 ----------------------PDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEM- 433
                                     K I I  +H+L  + VSAW MK L++ + +  + 
Sbjct: 451 KSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLT 510

Query: 434 SVSQILDDESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVN 493
           ++ + L D S  D     I +E + AK AA++IF NVA PG+KFI   D++ F+  +E  
Sbjct: 511 TLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEAL 570

Query: 494 LVWPHFE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVT 553
                FE   +T +I   +L NWVV  ++ R+ LA  L DTKTAV +L+ ++  ++ ++ 
Sbjct: 571 KTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIII 630

Query: 554 AVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDG 613
            VIWL+++ I +TK LV + +Q+ V AF+FGN CK  FE++I++FV+HPFDVGDRC +DG
Sbjct: 631 LVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDG 690

Query: 614 VQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVER 673
           VQ++VEEMNILTTVFL+ +N+KV YPNS+L TK I NYYRSPDMGD +EFSI   TP E+
Sbjct: 691 VQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEK 750

Query: 674 IGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTK 716
           I  +K++I  Y+E    HWYP   +V K++E++N ++IA++  H MN QD  EK  RR++
Sbjct: 751 IILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQ 810

BLAST of CSPI04G25680 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 3.0e-123
Identity = 256/618 (41.42%), Postives = 376/618 (60.84%), Query Frame = 0

Query: 138 QPEGED---DGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVW 197
           + E ED   D ++    K  K+     ++WI +  I++ LV SLT++ L+ +  W L +W
Sbjct: 244 EEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLW 303

Query: 198 KWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLAT 257
           KW +   V++CG + + W++ ++VFL+E+NF  +K+VLYFV+G++KSVQ  LWL LVL  
Sbjct: 304 KWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLA 363

Query: 258 WGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFD 317
           W  LFD++  +   +R TA  L  VT  L   L+  ++WL+KT+L+K+LAS FHM+ +FD
Sbjct: 364 WHFLFDKK--VERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 423

Query: 318 RIQESLFLHHVLQTLLRPPLVEDE-------------------------STAKFRCCRFC 377
           RIQESLF  +V++TL  PPL+E +                            K     F 
Sbjct: 424 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 483

Query: 378 FESKKPDRKKV----------IDIGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILD 437
              K P   ++          I I ++  +  + VSAW MK L++ +    +S + Q + 
Sbjct: 484 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 543

Query: 438 DESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE 497
           D +  D     I +E + AK AA++IF NV  PG+++I   D L F+  EE       FE
Sbjct: 544 DTTQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 603

Query: 498 -VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLL 557
              ++ KI    L NWVVK ++ R+ LA  L DTKTAV +L+ +I  +I ++  +IWLL+
Sbjct: 604 GASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 663

Query: 558 MEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEE 617
           + IATT+ L+ L +QL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEE
Sbjct: 664 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 723

Query: 618 MNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQ 677
           MNILTTVFL+ +N+K+ YPNSVL TKPI NYYRSPDMGD VEF +   TP E+I A+K++
Sbjct: 724 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 783

Query: 678 IKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKR 716
           I  Y++    +WYP   +V   ++++N +KIA++  H MN QD  E+  RR  L+ E+ +
Sbjct: 784 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 843

BLAST of CSPI04G25680 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 434.9 bits (1117), Expect = 1.8e-120
Identity = 249/614 (40.55%), Postives = 382/614 (62.21%), Query Frame = 0

Query: 139 PEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCL 198
           P  E+D  +    K  K+   T+++W+ +  II+ L  SL++   K   +W L +WKW +
Sbjct: 281 PLAEED--VPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEV 340

Query: 199 LATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSL 258
              V++CG + + W + +VVF IERNFLL+K+VLYFV+G++++VQ  LWL LVL  W  L
Sbjct: 341 FLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFL 400

Query: 259 FDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFDRIQE 318
           FD++    + SR     L  VT  L  FL+  +LWLIKTL++K+LAS FH++ +FDRIQE
Sbjct: 401 FDKKVQRETRSR----FLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQE 460

Query: 319 SLFLHHVLQTLLRPPLV------EDESTAK---FR-----------CCRFCFESKKPDR- 378
           +LF  +V++TL  PP++      E+E  A+   F+            C   F   K  R 
Sbjct: 461 ALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRV 520

Query: 379 ----------KKVIDIG----KIHELKREKVSAWTMKVLVDAVTS-SEMSVSQILDDESY 438
                     K   D G     +H +  + +SAW MK L+  V + S  ++ + + + +Y
Sbjct: 521 MNPKLSPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTY 580

Query: 439 RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFE-VDK 498
            D +   I +E K AK AA++IFKNV   G K+I   DL+ F+  +E       FE   +
Sbjct: 581 EDESTRQIRSE-KEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPE 640

Query: 499 TRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIA 558
            ++I   AL NW+V  ++ R+ LA  L DTKTAV +L+++I  +  +V  VIWL+L+EIA
Sbjct: 641 NKRISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIA 700

Query: 559 TTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNIL 618
           ++KVL+F+ +Q+ + AF+FGNT KT FE++IF+F++HP+DVGDRC +D VQL+VEEMNIL
Sbjct: 701 SSKVLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNIL 760

Query: 619 TTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRY 678
           TTVFL+ +N K+ YPNS+L  K I NYYRSPDMGD +EF +   TP+E+I  +K++I  Y
Sbjct: 761 TTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNY 820

Query: 679 LEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEE 716
           ++  P++WYP   ++VK++E+++ +++A++  H +N QD AE+  RR  LV E+ +I  E
Sbjct: 821 IDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLE 880

BLAST of CSPI04G25680 vs. ExPASy TrEMBL
Match: A0A0A0L5E6 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 PE=3 SV=1)

HSP 1 Score: 1396.7 bits (3614), Expect = 0.0e+00
Identity = 719/721 (99.72%), Postives = 720/721 (99.86%), Query Frame = 0

Query: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
           MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA
Sbjct: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60

Query: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
           SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE
Sbjct: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120

Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
           QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
           NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGA LWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
           KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360

Query: 361 IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
           ID+GKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
           AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
           ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
           ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720

Query: 721 H 722
           H
Sbjct: 721 H 721

BLAST of CSPI04G25680 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 686/721 (95.15%), Postives = 696/721 (96.53%), Query Frame = 0

Query: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
           MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DSQTKEPTGSSVGY NA
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
           S LAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENHVSLRE
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
           QIGATSSRSSNLNTPKAQPEGED  NMG N KHKK KVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGA LWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
           KILASKFHMNRFFDRIQESLFLHHVLQTLLRPP VEDESTAKFRCCRFCFESK+ DRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
           ID+GKIH+LKREKVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVADGDITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
           AKEIFKNVALPGNKFIEERDLL FMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALII+VTA+IWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
           ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
           ENVNKIKIALYTNHTMNFQDWAEKNRRRT+LVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELKINYNLLPQTVHLFPVEG 720

Query: 721 H 722
           H
Sbjct: 721 H 721

BLAST of CSPI04G25680 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 686/721 (95.15%), Postives = 696/721 (96.53%), Query Frame = 0

Query: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
           MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DSQTKEPTGSSVGY NA
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
           S LAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENHVSLRE
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
           QIGATSSRSSNLNTPKAQPEGED  NMG N KHKK KVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGA LWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
           KILASKFHMNRFFDRIQESLFLHHVLQTLLRPP VEDESTAKFRCCRFCFESK+ DRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
           ID+GKIH+LKREKVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVADGDITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
           AKEIFKNVALPGNKFIEERDLL FMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALII+VTA+IWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
           ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
           ENVNKIKIALYTNHTMNFQDWAEKNRRRT+LVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELKINYNLLPQTVHLFPVEG 720

Query: 721 H 722
           H
Sbjct: 721 H 721

BLAST of CSPI04G25680 vs. ExPASy TrEMBL
Match: A0A5D3D919 (Mechanosensitive ion channel protein 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001770 PE=4 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 4.0e-309
Identity = 570/713 (79.94%), Postives = 621/713 (87.10%), Query Frame = 0

Query: 6   NKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAP 65
           +K + R S  KES +GG+ VV+LS +EN CSVP+QN   SQT E   SS+ YDN S LA 
Sbjct: 10  SKVVPRSSFPKESEDGGQFVVELSSIENGCSVPEQN-LGSQTNEFIDSSISYDNDSQLA- 69

Query: 66  TPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGAT 125
             NKP  IP SNG LT    LRR+ LSK KSRFG Q  + DS+M EEEN+ S REQIG T
Sbjct: 70  --NKPQNIPSSNGNLT----LRRAILSKSKSRFGVQPVYTDSNMCEEENYPSSREQIGET 129

Query: 126 SSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKN 185
           SSRS   NT KA PE +D+       KHKKVKVKTVIKWIGVFCIISCLVASLTV+PLKN
Sbjct: 130 SSRSFTHNTQKATPERKDE-------KHKKVKVKTVIKWIGVFCIISCLVASLTVDPLKN 189

Query: 186 RFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVT 245
           RFLWGLKVWKWCLLATVILCGL+FTRWVMNVVVFLIE+NFLLKKKVLYFVHGLKKSVQVT
Sbjct: 190 RFLWGLKVWKWCLLATVILCGLLFTRWVMNVVVFLIEKNFLLKKKVLYFVHGLKKSVQVT 249

Query: 246 LWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILAS 305
           LWL+LV ATW SLFDRRNH +S+SRIT+K+LD VTWTL S LIGA LWLIKTLLLKILAS
Sbjct: 250 LWLTLVFATWESLFDRRNHTVSNSRITSKVLDFVTWTLVSLLIGAFLWLIKTLLLKILAS 309

Query: 306 KFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKVIDIGK 365
           KFHMNRFFDRIQESLF HH+LQ LL     EDES A+FRCCRF  ESKK D +KVIDI K
Sbjct: 310 KFHMNRFFDRIQESLFHHHILQMLLMARTQEDESYAEFRCCRFPSESKKSDCQKVIDIEK 369

Query: 366 IHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEAAKEIF 425
           IH+LKREKVSAW MK LVDAVTSSEMS+S+ L DESYR+ ADG+IT+EMKVAK+AAK+IF
Sbjct: 370 IHQLKREKVSAWKMKTLVDAVTSSEMSISKAL-DESYRNAADGEITDEMKVAKQAAKKIF 429

Query: 426 KNVALPGNKFIEERDLLEFMIPE-EVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTL 485
           KNVA PG KF+EE+DLL+FMI E EVNL+WPHFEVDKT+KIDMKALTNWVVKVYQGRKTL
Sbjct: 430 KNVA-PGKKFLEEKDLLKFMIDEAEVNLLWPHFEVDKTKKIDMKALTNWVVKVYQGRKTL 489

Query: 486 AHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTC 545
           AHALKDTKTAVKQL+NL+ ALI++VTAVIWLLLMEIATTKVLVFLLTQ AVAAFMFGNTC
Sbjct: 490 AHALKDTKTAVKQLDNLVAALIVIVTAVIWLLLMEIATTKVLVFLLTQFAVAAFMFGNTC 549

Query: 546 KTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP 605
           K  FE LIFVFVMHPFDVGD CVVDG+QLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP
Sbjct: 550 KNTFEGLIFVFVMHPFDVGDLCVVDGIQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKP 609

Query: 606 ITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVN 665
           ITNYYRSPDMGDT+EFSI F TP+E+IG MKE+IKRYLE+NPQHWYPNH+VVVKEIENVN
Sbjct: 610 ITNYYRSPDMGDTIEFSISFTTPLEKIGIMKEKIKRYLEDNPQHWYPNHSVVVKEIENVN 669

Query: 666 KIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFP 718
           KIKIALYTNHTMNFQDW EKNRRRT+L+MELKRIFEELKINYNLLPQTVHLFP
Sbjct: 670 KIKIALYTNHTMNFQDWTEKNRRRTELMMELKRIFEELKINYNLLPQTVHLFP 705

BLAST of CSPI04G25680 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 5.9e-300
Identity = 565/752 (75.13%), Postives = 631/752 (83.91%), Query Frame = 0

Query: 1   MDVSGN---KGIRRGSSQKESGNGGEVVVQLSGV--------ENECSVPKQNR-ADSQTK 60
           MDV+GN   K +RR SSQKES NGG+VVV++SGV        EN CSVP QNR  DSQ K
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  EPTGSSVGYDNASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQS-CFIDS 120
            PT SS+G         + NKPPKIP S+GTL PR+SL+RS  SKPKSRFGEQ   +IDS
Sbjct: 61  GPTDSSIG---------SANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDS 120

Query: 121 DMLEEENHVSLREQIGATSSR------SSNLNTPKAQPEGEDDGNMG-----SNAKHKKV 180
           DM EE+  +SLREQIGATSSR       S L TP+AQ E ED+  +      S  KHKK+
Sbjct: 121 DMFEED-RLSLREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKKL 180

Query: 181 KVKTVIKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNV 240
           K+KT++KW+GVFCII CLVASLTVN L+N FLWGL++WKWCLLATVILCG+IFT+WVMNV
Sbjct: 181 KMKTLVKWVGVFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMNV 240

Query: 241 VVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKIL 300
           +VFLIERNFLLKKKVLYFVHGLKK VQVTLWL+LVLATW SLFDR NH +  S+   KIL
Sbjct: 241 IVFLIERNFLLKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKIL 300

Query: 301 DAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLV- 360
           DA TWTL S LIGA LWL+KTLLLKILASKFHMNRFFDRIQES+F HHVLQTL RPPL+ 
Sbjct: 301 DAGTWTLVSLLIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLMG 360

Query: 361 --EDESTAKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMSV 420
             E  ++AK    R   + KK D KKVID+GKIH+LKREKVSAWTMKVLVDAVTSSE+S+
Sbjct: 361 VAECAASAKSSSGRLSLKGKKSDHKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELSI 420

Query: 421 SQILDDESYR----DVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEE 480
           SQ+L DESY+    +VAD DIT+EM+VA+ AA  IF NVALPGNKFIEE DLL+FMI EE
Sbjct: 421 SQLL-DESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMIKEE 480

Query: 481 VNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVV 540
           V+LV P FEV  TR+ID KALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNL+TAL+I+V
Sbjct: 481 VDLVLPLFEVADTRQIDRKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALLIIV 540

Query: 541 TAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVD 600
           TAV+WLLLMEIATTKVLVFLL+QLAVAAFMFGNTCKT FEALIFVFVMHPFDVGDRCVVD
Sbjct: 541 TAVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDRCVVD 600

Query: 601 GVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVE 660
           GV LLVEEMNILTTVFLKL+NEKVYYPNSVL+TKPITNYYRSPDM DTVEFSI F TP+E
Sbjct: 601 GVPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFTTPLE 660

Query: 661 RIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRT 720
           RIGAMKE+IKRYLE+N QHW+PNH+VVVKEIE+VNKIKIALY NHT+NFQ+WAEKNRRRT
Sbjct: 661 RIGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKNRRRT 720

Query: 721 KLVMELKRIFEELKINYNLLPQTVHLFPVEGH 722
           +LVMELK+IFEEL INYNLLPQTVHLFPVE H
Sbjct: 721 ELVMELKKIFEELNINYNLLPQTVHLFPVEAH 741

BLAST of CSPI04G25680 vs. NCBI nr
Match: KAE8649918.1 (hypothetical protein Csa_012502 [Cucumis sativus])

HSP 1 Score: 1396.7 bits (3614), Expect = 0.0e+00
Identity = 719/721 (99.72%), Postives = 720/721 (99.86%), Query Frame = 0

Query: 1    MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
            MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA
Sbjct: 738  MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 797

Query: 61   SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
            SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE
Sbjct: 798  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 857

Query: 121  QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
            QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV
Sbjct: 858  QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 917

Query: 181  NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
            NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 918  NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 977

Query: 241  SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
            SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGA LWLIKTLLL
Sbjct: 978  SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 1037

Query: 301  KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
            KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV
Sbjct: 1038 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 1097

Query: 361  IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
            ID+GKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA
Sbjct: 1098 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 1157

Query: 421  AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
            AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 1158 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 1217

Query: 481  RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
            RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 1218 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 1277

Query: 541  GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
            GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 1278 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 1337

Query: 601  ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
            ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 1338 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 1397

Query: 661  ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
            ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 1398 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 1457

Query: 721  H 722
            H
Sbjct: 1458 H 1458

BLAST of CSPI04G25680 vs. NCBI nr
Match: XP_004141433.2 (mechanosensitive ion channel protein 10 [Cucumis sativus])

HSP 1 Score: 1396.7 bits (3614), Expect = 0.0e+00
Identity = 719/721 (99.72%), Postives = 720/721 (99.86%), Query Frame = 0

Query: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
           MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA
Sbjct: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60

Query: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
           SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE
Sbjct: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120

Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
           QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
           NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGA LWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
           KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360

Query: 361 IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
           ID+GKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
           AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
           ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
           ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720

Query: 721 H 722
           H
Sbjct: 721 H 721

BLAST of CSPI04G25680 vs. NCBI nr
Match: XP_008452779.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_016901266.1 PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >KAA0064530.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK20060.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 686/721 (95.15%), Postives = 696/721 (96.53%), Query Frame = 0

Query: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60
           MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DSQTKEPTGSSVGY NA
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120
           S LAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENHVSLRE
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180
           QIGATSSRSSNLNTPKAQPEGED  NMG N KHKK KVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLKKKVLYFVHGLKK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGA LWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360
           KILASKFHMNRFFDRIQESLFLHHVLQTLLRPP VEDESTAKFRCCRFCFESK+ DRKKV
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 IDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420
           ID+GKIH+LKREKVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVADGDITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480
           AKEIFKNVALPGNKFIEERDLL FMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALII+VTA+IWLLLMEIATTKVLVFLLTQLAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660
           ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

Query: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQTVHLFPVEG 720
           ENVNKIKIALYTNHTMNFQDWAEKNRRRT+LVMELKRIFEELKINYNLLPQTVHLFPVEG
Sbjct: 661 ENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELKINYNLLPQTVHLFPVEG 720

Query: 721 H 722
           H
Sbjct: 721 H 721

BLAST of CSPI04G25680 vs. NCBI nr
Match: XP_038899727.1 (mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida] >XP_038899728.1 mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 598/729 (82.03%), Postives = 648/729 (88.89%), Query Frame = 0

Query: 1   MDVSGNKGIR--RGS-SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGY 60
           MDV+ N+ ++   GS SQ ES NGG+V V +S VEN  SVPKQNR DSQTKEP  SS+GY
Sbjct: 1   MDVNSNRALKVVGGSLSQMESENGGQVAVNISSVENGYSVPKQNRVDSQTKEPIDSSIGY 60

Query: 61  DNASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVS 120
              S LAPT NKPPKIP S GTLTPRRSLRR  LSK KSRFGEQ  +IDSD+  EENHVS
Sbjct: 61  SYDSQLAPTANKPPKIPSSKGTLTPRRSLRRPILSKSKSRFGEQPGYIDSDVF-EENHVS 120

Query: 121 LREQIGATSSRSSNLNTPKAQPEGEDDGNMGS----NAKHKKVKVKTVIKWIGVFCIISC 180
           LREQIG+TSSRSS +NTPKAQPE EDD  +      N KHKKVKVKTVI+WIGVFCII C
Sbjct: 121 LREQIGSTSSRSSTVNTPKAQPEEEDDAGIFEIEQLNNKHKKVKVKTVIEWIGVFCIIGC 180

Query: 181 LVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLY 240
           LVASLTVNP KNRFLWGL+VWKW LLATVILCG+IFTRWVMNV+VFLIERNFLLKKKVLY
Sbjct: 181 LVASLTVNPWKNRFLWGLRVWKWLLLATVILCGVIFTRWVMNVIVFLIERNFLLKKKVLY 240

Query: 241 FVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLW 300
           FVHGLKKSVQVTLWL+LVL TWGSLFD+RNH I SSRITAKILD+VTWTL S LIGAVL 
Sbjct: 241 FVHGLKKSVQVTLWLTLVLVTWGSLFDQRNHKI-SSRITAKILDSVTWTLVSLLIGAVLL 300

Query: 301 LIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLV-EDESTAKFRCCRFCFES 360
           L+KTLLLKILASKFHMNRFFDR+QES+FLH+VLQTL R   + E  +T+K    R   +S
Sbjct: 301 LVKTLLLKILASKFHMNRFFDRVQESIFLHYVLQTLSRGVFIGEAGTTSKSSSGRLSLKS 360

Query: 361 KKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITN 420
           KK DRK+VID+GKIH+LKREKVSAWTMKVLVDAVTSSEMSVSQIL DESY+DVADG+I +
Sbjct: 361 KKSDRKEVIDLGKIHQLKREKVSAWTMKVLVDAVTSSEMSVSQIL-DESYQDVADGEIAD 420

Query: 421 EMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTN 480
           EM+VAK AA +IF NVALPGNKFIEE DLL+FMI EEV+LV PHFE+D TR+ID KALTN
Sbjct: 421 EMEVAKAAAYKIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLPHFEIDDTRRIDKKALTN 480

Query: 481 WVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQ 540
           WVVKVYQGRKTLAHALKDTKTAVKQLNNL+TALII+VTAVIWLLLMEIATTKVLVFLLTQ
Sbjct: 481 WVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALIIIVTAVIWLLLMEIATTKVLVFLLTQ 540

Query: 541 LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK 600
           LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK
Sbjct: 541 LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK 600

Query: 601 VYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPN 660
           VYYPNSVLATKPITNYYRSPDMG+T+EFSIGF TP+ERIG MK++IKRYLE+N QHWYPN
Sbjct: 601 VYYPNSVLATKPITNYYRSPDMGETIEFSIGFTTPLERIGVMKDKIKRYLEKNSQHWYPN 660

Query: 661 HNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQT 720
           H+VVV+EIENVNKIKIALYTNHTMNFQDW EKNRRRT+LVMELKRIFEELKINYNLLPQT
Sbjct: 661 HSVVVQEIENVNKIKIALYTNHTMNFQDWTEKNRRRTELVMELKRIFEELKINYNLLPQT 720

Query: 721 VHLFPVEGH 722
           VHL  VEGH
Sbjct: 721 VHLIQVEGH 726

BLAST of CSPI04G25680 vs. NCBI nr
Match: XP_038899729.1 (mechanosensitive ion channel protein 10-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 596/729 (81.76%), Postives = 646/729 (88.61%), Query Frame = 0

Query: 1   MDVSGNKGIR--RGS-SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGY 60
           MDV+ N+ ++   GS SQ ES NGG+V V +S VEN  SVPKQNR DSQTKEP  SS+GY
Sbjct: 1   MDVNSNRALKVVGGSLSQMESENGGQVAVNISSVENGYSVPKQNRVDSQTKEPIDSSIGY 60

Query: 61  DNASPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVS 120
              S LAPT NKPPKIP S GTLTPRRSLRR  LSK KSRFGEQ  +IDSD+  EENHVS
Sbjct: 61  SYDSQLAPTANKPPKIPSSKGTLTPRRSLRRPILSKSKSRFGEQPGYIDSDVF-EENHVS 120

Query: 121 LREQIGATSSRSSNLNTPKAQPEGEDDGNMGS----NAKHKKVKVKTVIKWIGVFCIISC 180
           LREQIG+TSSRSS +NTPKAQPE EDD  +      N KHKKVKVKTVI+WIGVFCII C
Sbjct: 121 LREQIGSTSSRSSTVNTPKAQPEEEDDAGIFEIEQLNNKHKKVKVKTVIEWIGVFCIIGC 180

Query: 181 LVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLY 240
           LVASLTVNP KNRFLWGL+VWKW LLATVILCG+IFTRWVMNV+VFLIERNFLLKKKVLY
Sbjct: 181 LVASLTVNPWKNRFLWGLRVWKWLLLATVILCGVIFTRWVMNVIVFLIERNFLLKKKVLY 240

Query: 241 FVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLW 300
           FVHGLKKSVQVTLWL+LVL TWGSLFD+RNH I SSRITAKILD+VTWTL S LIGAVL 
Sbjct: 241 FVHGLKKSVQVTLWLTLVLVTWGSLFDQRNHKI-SSRITAKILDSVTWTLVSLLIGAVLL 300

Query: 301 LIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLV-EDESTAKFRCCRFCFES 360
           L+KTLLLKILASKFHMNRFFDR+QES+FLH+VLQTL R   + E  +T+K    R   +S
Sbjct: 301 LVKTLLLKILASKFHMNRFFDRVQESIFLHYVLQTLSRGVFIGEAGTTSKSSSGRLSLKS 360

Query: 361 KKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITN 420
           KK DRK+VID+GKIH+LKREKVSAWTMKVLVDAVTSSEMSVSQIL DESY+DVADG+I +
Sbjct: 361 KKSDRKEVIDLGKIHQLKREKVSAWTMKVLVDAVTSSEMSVSQIL-DESYQDVADGEIAD 420

Query: 421 EMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTN 480
           EM+VAK AA +IF NVALPGNKFIEE DLL+FMI EEV+LV PHFE+D TR+ID KALTN
Sbjct: 421 EMEVAKAAAYKIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLPHFEIDDTRRIDKKALTN 480

Query: 481 WVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQ 540
           WV  VYQGRKTLAHALKDTKTAVKQLNNL+TALII+VTAVIWLLLMEIATTKVLVFLLTQ
Sbjct: 481 WV--VYQGRKTLAHALKDTKTAVKQLNNLVTALIIIVTAVIWLLLMEIATTKVLVFLLTQ 540

Query: 541 LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK 600
           LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK
Sbjct: 541 LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEK 600

Query: 601 VYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPN 660
           VYYPNSVLATKPITNYYRSPDMG+T+EFSIGF TP+ERIG MK++IKRYLE+N QHWYPN
Sbjct: 601 VYYPNSVLATKPITNYYRSPDMGETIEFSIGFTTPLERIGVMKDKIKRYLEKNSQHWYPN 660

Query: 661 HNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKINYNLLPQT 720
           H+VVV+EIENVNKIKIALYTNHTMNFQDW EKNRRRT+LVMELKRIFEELKINYNLLPQT
Sbjct: 661 HSVVVQEIENVNKIKIALYTNHTMNFQDWTEKNRRRTELVMELKRIFEELKINYNLLPQT 720

Query: 721 VHLFPVEGH 722
           VHL  VEGH
Sbjct: 721 VHLIQVEGH 724

BLAST of CSPI04G25680 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 662.1 bits (1707), Expect = 4.9e-190
Identity = 385/740 (52.03%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
           S    G GG+VV+ +  VE      K+  +    K    S       S L  +PNKPP+ 
Sbjct: 6   SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65

Query: 74  PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
           P  N   LT R+S  RS  SKPKSRF + SC +D+ +LEEE    +REQ+GA  S S   
Sbjct: 66  PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125

Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
              +A P  + + ++GS A                 +KKVK+            +I+   
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185

Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
              I+S LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245

Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
           L++KVLYFVHGLKKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S 
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305

Query: 314 LIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
           L GA  WL+KTLLLKILA+ F++N FFDRIQ+S+F  +VLQTL   PL+E+      E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365

Query: 374 AKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
                     +      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S +S  LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425

Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
            +Y   ++ AD +IT+EM+ A  AA  +F+NVA P   +IEE DLL FMI EEV+LV+P 
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485

Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
           F+   +T +I  KA T WVVKVY  R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545

Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
           LL+E+ATTKVL+F  TQL   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605

Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
           EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I  +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665

Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMEL 716
           E+I  YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+  E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725

BLAST of CSPI04G25680 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 662.1 bits (1707), Expect = 4.9e-190
Identity = 385/740 (52.03%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
           S    G GG+VV+ +  VE      K+  +    K    S       S L  +PNKPP+ 
Sbjct: 6   SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65

Query: 74  PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
           P  N   LT R+S  RS  SKPKSRF + SC +D+ +LEEE    +REQ+GA  S S   
Sbjct: 66  PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125

Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
              +A P  + + ++GS A                 +KKVK+            +I+   
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185

Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
              I+S LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245

Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
           L++KVLYFVHGLKKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S 
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305

Query: 314 LIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
           L GA  WL+KTLLLKILA+ F++N FFDRIQ+S+F  +VLQTL   PL+E+      E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365

Query: 374 AKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
                     +      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S +S  LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425

Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
            +Y   ++ AD +IT+EM+ A  AA  +F+NVA P   +IEE DLL FMI EEV+LV+P 
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485

Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
           F+   +T +I  KA T WVVKVY  R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545

Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
           LL+E+ATTKVL+F  TQL   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605

Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
           EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I  +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665

Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMEL 716
           E+I  YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+  E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725

BLAST of CSPI04G25680 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 662.1 bits (1707), Expect = 4.9e-190
Identity = 385/740 (52.03%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPLAPTPNKPPKI 73
           S    G GG+VV+ +  VE      K+  +    K    S       S L  +PNKPP+ 
Sbjct: 6   SSNGGGGGGDVVINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRA 65

Query: 74  PISNGT-LTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLREQIGATSSRSSNL 133
           P  N   LT R+S  RS  SKPKSRF + SC +D+ +LEEE    +REQ+GA  S S   
Sbjct: 66  PNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VREQLGAGFSFS--- 125

Query: 134 NTPKAQPEGEDDGNMGSNAK----------------HKKVKVK----------TVIKWIG 193
              +A P  + + ++GS A                 +KKVK+            +I+   
Sbjct: 126 ---RASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAF 185

Query: 194 VFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFL 253
              I+S LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFL
Sbjct: 186 FVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFL 245

Query: 254 LKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASF 313
           L++KVLYFVHGLKKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S 
Sbjct: 246 LRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISI 305

Query: 314 LIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVED------EST 373
           L GA  WL+KTLLLKILA+ F++N FFDRIQ+S+F  +VLQTL   PL+E+      E +
Sbjct: 306 LTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPS 365

Query: 374 AKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDD 433
                     +      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S +S  LD+
Sbjct: 366 TGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDE 425

Query: 434 ESY---RDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPH 493
            +Y   ++ AD +IT+EM+ A  AA  +F+NVA P   +IEE DLL FMI EEV+LV+P 
Sbjct: 426 TAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPL 485

Query: 494 FE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWL 553
           F+   +T +I  KA T WVVKVY  R+ LAH+L DTKTAVKQLN L+TA+++VVT VIWL
Sbjct: 486 FDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWL 545

Query: 554 LLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLV 613
           LL+E+ATTKVL+F  TQL   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LV
Sbjct: 546 LLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLV 605

Query: 614 EEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMK 673
           EEMN+LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+TVEFSI F TPV +I  +K
Sbjct: 606 EEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLK 665

Query: 674 EQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMEL 716
           E+I  YLE+NPQHW P H+VVVKEIEN+NK+K+ALY++HT+ FQ+  E+N RRT+L + +
Sbjct: 666 ERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAI 725

BLAST of CSPI04G25680 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 607.1 bits (1564), Expect = 1.9e-173
Identity = 355/748 (47.46%), Postives = 487/748 (65.11%), Query Frame = 0

Query: 14  SQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNASPL----APTP-- 73
           +++   NG EVV+ +S  E +   P+ + + +    P  S  G + + P+     PTP  
Sbjct: 2   AERRVSNGEEVVINVSDKE-DSKDPRASPSFNPLASP-DSDAGIEKSKPVPPISIPTPEI 61

Query: 74  -------NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 133
                  +KPPKIP   G L  R+SL RS  SKPKSRFGEQ  F      EE    SLRE
Sbjct: 62  YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121

Query: 134 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAK--------------HKKVKVKTV----- 193
           Q GA S    + +  +A P  + + ++ S A               +KKVK+  V     
Sbjct: 122 QFGAGSFARGSFD--RASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGM 181

Query: 194 -----IKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNV 253
                ++ +    I+  L+ SLT++ +    +WGL+ WKWC+L  V L G++ T W M+ 
Sbjct: 182 KPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHF 241

Query: 254 VVFLIERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKIL 313
           VVF+IE+N+LL+KKVLYFVHGLKK+VQV +W SLVL  W  LFD     +  +R T + L
Sbjct: 242 VVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFL 301

Query: 314 DAVTWTLASFLIGAVLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVE 373
           D +TWT+ S L+G++L+L+KT  LK+LASKF++  FF+RIQES+F  +VLQTL  PPL+E
Sbjct: 302 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 361

Query: 374 D-------ESTAKFRCCRFCFESKKPDRKKVIDIGKIHELKREKVSAWTMKVLVDAV-TS 433
           +        ST      R   +  K   KKVID+GK+H +K+EKVSAWTM+VL++AV TS
Sbjct: 362 EAENVGRVPSTGHLSFTR--TKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTS 421

Query: 434 SEMSVSQILDD-ESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIP 493
              ++S  LD+  + ++  D +ITNEM+ A  AA ++F NVA P + +IEE DLL FMI 
Sbjct: 422 GISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIK 481

Query: 494 EEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALII 553
           EEV+LV P  E   T KI  K  T WVV VY  RKT+ H+L DTKTAVKQL+ LIT ++ 
Sbjct: 482 EEVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILT 541

Query: 554 VVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCV 613
           V+T ++W++L++IA+TK+L+   +Q    AFM G+TCK  FE+ +FVFVMHP+DVGDRCV
Sbjct: 542 VITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCV 601

Query: 614 VDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETP 673
           VDGV LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD V+F I F TP
Sbjct: 602 VDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTP 661

Query: 674 VERIGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRR 716
            E+IG +K +I  YL  N QHWYP   V+V+ IEN+NK+ + +   HT+NFQ + EK+ R
Sbjct: 662 AEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLR 721

BLAST of CSPI04G25680 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 455.7 bits (1171), Expect = 7.0e-128
Identity = 259/625 (41.44%), Postives = 382/625 (61.12%), Query Frame = 0

Query: 134 TPKAQPEGEDDG-----NMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTVNPLKNRFL 193
           +PK Q E E+D      ++    +  K+ +  V++W+ +  II+  V +L +  L+ + L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270

Query: 194 WGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKKSVQVTLWL 253
           W L++WKW  +  V++CG + + W++ +VVF IERNFLL+K+VLYFV+G++K+VQ  LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330

Query: 254 SLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAVLWLIKTLLLKILASKFH 313
            LVL  W  LFD +     +     K L  VT      L+G +LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEK----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390

Query: 314 MNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKK-------------- 373
           M+ +FDRIQESLF  +V++TL  PPL+E +   +    R   E KK              
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE-ERISVEVKKFQNPGGVEIQSGAQ 450

Query: 374 ----------------------PDRKKVIDIGKIHELKREKVSAWTMKVLVDAVTSSEM- 433
                                     K I I  +H+L  + VSAW MK L++ + +  + 
Sbjct: 451 KSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLT 510

Query: 434 SVSQILDDESYRDVADGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVN 493
           ++ + L D S  D     I +E + AK AA++IF NVA PG+KFI   D++ F+  +E  
Sbjct: 511 TLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEAL 570

Query: 494 LVWPHFE-VDKTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVT 553
                FE   +T +I   +L NWVV  ++ R+ LA  L DTKTAV +L+ ++  ++ ++ 
Sbjct: 571 KTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIII 630

Query: 554 AVIWLLLMEIATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDG 613
            VIWL+++ I +TK LV + +Q+ V AF+FGN CK  FE++I++FV+HPFDVGDRC +DG
Sbjct: 631 LVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDG 690

Query: 614 VQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVER 673
           VQ++VEEMNILTTVFL+ +N+KV YPNS+L TK I NYYRSPDMGD +EFSI   TP E+
Sbjct: 691 VQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEK 750

Query: 674 IGAMKEQIKRYLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTK 716
           I  +K++I  Y+E    HWYP   +V K++E++N ++IA++  H MN QD  EK  RR++
Sbjct: 751 IILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQ 810

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYG97.0e-18952.03Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M972.7e-17247.46Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9SYM19.9e-12741.44Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LH743.0e-12341.42Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
F4IME21.8e-12040.55Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 P... [more]
Match NameE-valueIdentityDescription
A0A0A0L5E60.0e+0099.72Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 ... [more]
A0A1S4DZ480.0e+0095.15Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=... [more]
A0A5D3D9910.0e+0095.15Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3D9194.0e-30979.94Mechanosensitive ion channel protein 10-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1C8Z85.9e-30075.13Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009... [more]
Match NameE-valueIdentityDescription
KAE8649918.10.0e+0099.72hypothetical protein Csa_012502 [Cucumis sativus][more]
XP_004141433.20.0e+0099.72mechanosensitive ion channel protein 10 [Cucumis sativus][more]
XP_008452779.10.0e+0095.15PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_01690... [more]
XP_038899727.10.0e+0082.03mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida] >XP_... [more]
XP_038899729.10.0e+0081.76mechanosensitive ion channel protein 10-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G12080.14.9e-19052.03mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.24.9e-19052.03mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.34.9e-19052.03mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.11.9e-17347.46mechanosensitive channel of small conductance-like 9 [more]
AT1G78610.17.0e-12841.44mechanosensitive channel of small conductance-like 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..61
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..87
NoneNo IPR availablePANTHERPTHR31618:SF20MECHANOSENSITIVE ION CHANNEL PROTEIN 10coord: 89..573
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 89..573

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G25680.1CSPI04G25680.1mRNA
CSPI04G25680.3CSPI04G25680.3mRNA
CSPI04G25680.2CSPI04G25680.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0050982 detection of mechanical stimulus
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity