CSPI04G25640 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G25640
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Omega-amidase
Location	Chr4: 22945917 .. 22950088 (+)
RNA-Seq Expression	CSPI04G25640
Synteny	CSPI04G25640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTCTGACGTATCATCTTCAATCGAAAGCATAGATTGGTATAAAACAAAATTCTCAAGAGACTGAAATGAAAGCAGCCATTTCTTCGTTGATAAGCTCCAGAAATTTCAATCTCCATCAATCCTCATTCTCCCGTTCCATTTTCCACCAGCCTTCCCTTCTTCTACCGTCTCGCTTTCCGGTCTCATCCACCGGCGGCGGCGGCGGCGGCGCCAATTTAACCACCCTGCGCCATAAGTTTCGTTTCCACTCCATCAAGTCCTCGGCGAGTATGGCATCATCCTTCAATCCCGAACAGGCTAGATCTCCACCGGCACTTCCGTTGCCGATCCCTCCCGTAACAAAGGTTAGTTTGTCAACTTGACTCATACTCAGCTCGGTTTTGTTTCGTTAACTAATGTGAGTGTCGATATTTCTGGCTTGTTTCGACGTAGTTTAAAATCGCGCTATGTCAATTGGCGGTGACGGCTGATAAGCAAAGGAATATTGAACATGCTCGTAAGGCCATTGAGGAGGCTGTGGAAAAAGGCGCCCAGCTTGTTGTATTGCCGGTCAGTTCTTAGGTTTTTTAAATTATTGTTAGTCTGGTGTAATTTAAGCTTTCCTGGTGGTATTTCGTGGTTTTAGAGTTTCTGTTCTGCTCGTGATTTTTTCTATTGTTCCATATTTCTGTGACAGGAAATATGGAATTGTCCTTATTCACCTGATTGCTTACCACTTTATGCCGAGGACATTGAAGCCGGTGGTGACGCCTCCCCTTCAACAGCCATGTTGTCGGAAGTTTCTCGCCGTTTAAAAGTCACAATAGTCGGCGGCTCAATTGTAGAGCGCTCGGGGGATAAAATTTATAATTCTTCTTGTGTTTTTGGTACTGACGGAAAGTTGAAAGCTAAACACAGGAAGGTACAAAATGATATACATACCTGATTTTTAAATCCTGTGATGGAATACTAGCATTCAATGACGCATTAGAAATATTTCCTTATTTGATTTTGGATGTGGGAAGCCTTGATGGGGCACTTCGTTTATTTAGTGTGAAACTTTTAATGTGTTCCTAATAGTTTCACTTATTTGATGTTTACATATTGGTGGTAGATGATATAATATTGAATTTACTTTCAGTCATTAGCTTAAGTTTTTTTATCAATAGGTGATTTAAGACTTGTTTTTCATAAAACTGATTCATCTTTTTCTAACTCGATAATGCTGAAAAGACTAAAGAATTCATACTTGACTTTCCACTGGTAAATTTAAAATCTCCACTTTTAGGGTGTTCCTCAGTTTGTCTTCTGAAACATTTTTCGAGTTTCTATTTTGTTGTTAGGAGATGGATTTAAAATGGTTATTAAATGCTCCAAGTGTTCTACAAGAGAACAAGTTAATGTTGTTTAGATTAATCAAATTTGTTTGAAGGATGGTTTTTTCTTGTTTAACTCACCACTCACGAGTTTATTGTCATTTTCATCTGCAGATTCATCTTTTTGATATTGATATCCCTGGAAAGATCACTTTTATTGAGTCCAAGACCATTGCAGCAGGTCAAACTCCTACTGTTGTAGACACAGGTCGGTTTGTGATTCTTTTACTCTTCATGGTGGTGGTTGTAAGTGTAAATGACACTTAAGCTTAACTGCAATTACGTTAATTCTTTTAGTGACTATGGTATTTAGAAACTAACTACTATTTGCTGGCTCATGCAGAAGTTGGACGTATTGGAATAGGAATCTGTTATGACATCCGTTTTCCAGAACTAGCAATGATGTATGCTGCAAGAGGTTTGTCCTTTTATTTCTGTTTCTTTTCCAAATTTTCTAGTCTCCTATGTACATCATGTCTTTTCTGTACTGAAGATCACCTTCATTAGCACCTTAGATTATTGCATATACTGTATGAATCATTTACAGTATGACAAAAGCAGTGGCTCTCCCTCTTTTTATATATTTTATTCCATGTGACATGATTAGTCCTATACAGTGAATTATATATTCTACCACAATATTCCTTTCTTTAAAAATTGTGCTTCCGTCTATGATATTAGCAGATGTGGATAATATGAGGACTCAACTTTTCAGTATCTATGTTAGTTATGGATCTCTTGTGTTGCATTTTTGTTAGAGCAGAGGCAAATTGTGTGTGCATAATTTGCCATGATAACTATGATCTGATAAAAGATGCATTTTTGACATGTCATCTGGTGGTATGCTTTAACTTATCAGACAGCCCATAGATTGTTGTGGTGCCCACACAAAATCAATCATGTTCATTTTAATTTTAATGTTGGCTATCTCGATAGAGCCATGTTCTGGTGCTGACACAGAAATTCTTAACATCAGGTGCTCACTTGATTTGCTATCCTGGGGCATTTAACATGACTACTGGACCATTGCACTGGGAGTTATTGCAGCGCTCAAGGTACAAGCTTCTGTCTAAATTTCTGCCTTCATGTTTTGTTGCTAATGTTTGCCGGCATGTCTTGATGTTGTGAACATGATGAGTAGTGTAGAGATGGTTTGATCTCCAAGATGGGTGGGTTTTAGTTGGGACTCGGCATCTGATGCCTAAAAATGCAGAATGAGTCCTGGGATATATTGACTACAACCACAAACAGTTGTGCGTACAGAGTATGTTGGGACCTGAGGTACTGGGTTGTCCTATTGATAAGTGTCATCTTTTGTGAGCCAAAGCCTTTTCCATCCTCTACATGACCAGGCCTAAAACCACTGTTACCTTTAAGAAAAAGGGGAAACATTTTTTTCACTTTCATTACAATGTGACCACTGTATAATTTGATTCTTTTGGTCTATTCTCCTTTAGATAATTTTTTTTGGGTCTACTCTATTACATCTTCTAAATGATTAGAAATTGTTTTACTTGTAAATCAGTTATTCTGTTTGTATTCTGTTATTTTGGTATATTTTCTTTTACTTCTTCCTCTGTTTTTACTAAGAGGATTACCTTGTAATCTCATGAATAGTGAATAATTGTCTGAACTCTTTAACATCTTGACCCATGTAATTAGTGATATACTCAAATCTGGAAGTGCATCAAATTGCATACTCCATTGTAGGGCTGTAGCTTATTGAAATACTAGATTGCTATAACTGATATTTTTGCGTTAATGTTTTATGCAGGGCTGTGGATAATCAGGTATGCTACAAGTCTTCAGTTTTTCTTGTTGCCCGTGTTATGCACAGACACATCAATATTGAAGAAAAAAATGATATCGATATGACATGATTGGTTGTGTGGCTATTTTTCTTTTTGGTTGAAGAAGGAGGTTGTGTAGCTGAATTTCTTTGACTATGATACTACTAGTTCTAAAATAACAATAATAATAACAATAACAATACAATCTTTTTCATTAAAAAAAAAAACTTCCATTAAGAAAAATGAGAAATGTGCAAGAGCATACCCCCAAAAAAACCCTCCAAAAGGGAGTCGCAAAGCACAACATGCATGAAGTTAATAATGCGATTCTTCAAAAATACTTGTTCATTGTTCAATCTTTGCTGCAGTCTTGCAGTTGCAGTATTTATGCGTAATAAATGGTTGCACTTAACGTCATTGAATGTAATATAGTTTCCAAAAACACAAGGAAGAATCTTTCCATCTTGTCTGTGAACAGAACAATCTTACATTTAATACTTTTTGTTGTTCAGTTGTATGTGGCAACGTGTTCACCTGCTCGAAATGCTGATGCTAGTTATGTAGCTTGGGGCCACTCAACCCTTGTTGGGCCGGTGAGTTGATGCTGAAGTTCACCTTTGCCATAATTCAATTATGCTATGCAACTGAAGCCATTATATATCTGTGTTTCAATTGGTCACTTTGGAATGTCTCAACTCTATTCATGGACGAACTGTGCCTTTTGTTGTAGCTTTATCATGTATTCTTTTCCACAAAATCATTACAGTATAATGCTTCTTTCAGTTTGGGGAAGTGCTAGCTACAACGGAACATGATGAAGCAATAGTCATATCTGAGATTGATTATTCCTTTATAGAGCTTAGAAGGTGATCCATCTTTCAAACCTTTTAAGAAGTGTTTTTCACATTATTCAACTTTCTCATTGATAGTGTGTTAAAACTATTCAGGACTAACCTACCGCTTCTGAAACAAAGGCGTGGTGACCTTTACCAGTTGGTAGATGCTCAGAGATTGAAATCCGAATGA

mRNA sequence

TGTCTGACGTATCATCTTCAATCGAAAGCATAGATTGGTATAAAACAAAATTCTCAAGAGACTGAAATGAAAGCAGCCATTTCTTCGTTGATAAGCTCCAGAAATTTCAATCTCCATCAATCCTCATTCTCCCGTTCCATTTTCCACCAGCCTTCCCTTCTTCTACCGTCTCGCTTTCCGGTCTCATCCACCGGCGGCGGCGGCGGCGGCGCCAATTTAACCACCCTGCGCCATAAGTTTCGTTTCCACTCCATCAAGTCCTCGGCGAGTATGGCATCATCCTTCAATCCCGAACAGGCTAGATCTCCACCGGCACTTCCGTTGCCGATCCCTCCCGTAACAAAGTTTAAAATCGCGCTATGTCAATTGGCGGTGACGGCTGATAAGCAAAGGAATATTGAACATGCTCGTAAGGCCATTGAGGAGGCTGTGGAAAAAGGCGCCCAGCTTGTTGTATTGCCGGAAATATGGAATTGTCCTTATTCACCTGATTGCTTACCACTTTATGCCGAGGACATTGAAGCCGGTGGTGACGCCTCCCCTTCAACAGCCATGTTGTCGGAAGTTTCTCGCCGTTTAAAAGTCACAATAGTCGGCGGCTCAATTGTAGAGCGCTCGGGGGATAAAATTTATAATTCTTCTTGTGTTTTTGGTACTGACGGAAAGTTGAAAGCTAAACACAGGAAGATTCATCTTTTTGATATTGATATCCCTGGAAAGATCACTTTTATTGAGTCCAAGACCATTGCAGCAGGTCAAACTCCTACTGTTGTAGACACAGAAGTTGGACGTATTGGAATAGGAATCTGTTATGACATCCGTTTTCCAGAACTAGCAATGATGTATGCTGCAAGAGGTGCTCACTTGATTTGCTATCCTGGGGCATTTAACATGACTACTGGACCATTGCACTGGGAGTTATTGCAGCGCTCAAGGGCTGTGGATAATCAGTTGTATGTGGCAACGTGTTCACCTGCTCGAAATGCTGATGCTAGTTATGTAGCTTGGGGCCACTCAACCCTTGTTGGGCCGTTTGGGGAAGTGCTAGCTACAACGGAACATGATGAAGCAATAGTCATATCTGAGATTGATTATTCCTTTATAGAGCTTAGAAGGACTAACCTACCGCTTCTGAAACAAAGGCGTGGTGACCTTTACCAGTTGGTAGATGCTCAGAGATTGAAATCCGAATGA

Coding sequence (CDS)

ATGAAAGCAGCCATTTCTTCGTTGATAAGCTCCAGAAATTTCAATCTCCATCAATCCTCATTCTCCCGTTCCATTTTCCACCAGCCTTCCCTTCTTCTACCGTCTCGCTTTCCGGTCTCATCCACCGGCGGCGGCGGCGGCGGCGCCAATTTAACCACCCTGCGCCATAAGTTTCGTTTCCACTCCATCAAGTCCTCGGCGAGTATGGCATCATCCTTCAATCCCGAACAGGCTAGATCTCCACCGGCACTTCCGTTGCCGATCCCTCCCGTAACAAAGTTTAAAATCGCGCTATGTCAATTGGCGGTGACGGCTGATAAGCAAAGGAATATTGAACATGCTCGTAAGGCCATTGAGGAGGCTGTGGAAAAAGGCGCCCAGCTTGTTGTATTGCCGGAAATATGGAATTGTCCTTATTCACCTGATTGCTTACCACTTTATGCCGAGGACATTGAAGCCGGTGGTGACGCCTCCCCTTCAACAGCCATGTTGTCGGAAGTTTCTCGCCGTTTAAAAGTCACAATAGTCGGCGGCTCAATTGTAGAGCGCTCGGGGGATAAAATTTATAATTCTTCTTGTGTTTTTGGTACTGACGGAAAGTTGAAAGCTAAACACAGGAAGATTCATCTTTTTGATATTGATATCCCTGGAAAGATCACTTTTATTGAGTCCAAGACCATTGCAGCAGGTCAAACTCCTACTGTTGTAGACACAGAAGTTGGACGTATTGGAATAGGAATCTGTTATGACATCCGTTTTCCAGAACTAGCAATGATGTATGCTGCAAGAGGTGCTCACTTGATTTGCTATCCTGGGGCATTTAACATGACTACTGGACCATTGCACTGGGAGTTATTGCAGCGCTCAAGGGCTGTGGATAATCAGTTGTATGTGGCAACGTGTTCACCTGCTCGAAATGCTGATGCTAGTTATGTAGCTTGGGGCCACTCAACCCTTGTTGGGCCGTTTGGGGAAGTGCTAGCTACAACGGAACATGATGAAGCAATAGTCATATCTGAGATTGATTATTCCTTTATAGAGCTTAGAAGGACTAACCTACCGCTTCTGAAACAAAGGCGTGGTGACCTTTACCAGTTGGTAGATGCTCAGAGATTGAAATCCGAATGA

Protein sequence

MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQLVDAQRLKSE*

Homology

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BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot
Match: Q8RUF8 (Omega-amidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NLP3 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.1e-148
Identity = 265/381 (69.55%), Postives = 306/381 (80.31%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MK+AISS +   + NL   +         S  LP   P S T             H  + 
Sbjct: 1   MKSAISSSLFFNSKNLLNPNPLSRFISLKSNFLPKLSPRSITS------------HTLKL 60

Query: 61  HSIKSSA------SMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHA 120
            S  +SA      SMASSFNPEQAR P ALPLP PP+TKF I LCQL+VT+DK+RNI HA
Sbjct: 61  PSSSTSALRSISSSMASSFNPEQARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHA 120

Query: 121 RKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVT 180
           +KAIEEA  KGA+LV+LPEIWN PYS D  P+YAE+I+AGGDASPSTAMLSEVS+RLK+T
Sbjct: 121 KKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKIT 180

Query: 181 IVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPT 240
           I+GGSI ER GD++YN+ CVFG+DG+LKAKHRKIHLFDIDIPGKITF+ESKT+ AG+TPT
Sbjct: 181 IIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPT 240

Query: 241 VVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDN 300
           +VDT+VGRIGIGICYDIRF ELAM+YAARGAHL+CYPGAFNMTTGPLHWELLQR+RA DN
Sbjct: 241 IVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDN 300

Query: 301 QLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLP 360
           QLYVATCSPAR++ A Y AWGHSTLVGPFGEVLATTEH+EAI+I+EIDYS +E RRT+LP
Sbjct: 301 QLYVATCSPARDSGAGYTAWGHSTLVGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLP 360

Query: 361 LLKQRRGDLYQLVDAQRLKSE 376
           L +QRRGDLYQLVD QRL S+
Sbjct: 361 LNRQRRGDLYQLVDVQRLDSK 369

[Close]

BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot
Match: Q9NQR4 (Omega-amidase NIT2 OS=Homo sapiens OX=9606 GN=NIT2 PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 7.3e-81
Identity = 150/274 (54.74%), Postives = 191/274 (69.71%), Query Frame = 0


Query: 91  VTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAED 150
           +T F++AL QL +++ K  N+  A   I EA  +GA++V LPE +N PY     P YAE 
Sbjct: 1   MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEK 60

Query: 151 IEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHL 210
           I        ST  LSEV++   + ++GGSI E    K+YN+  VFG DG L AK+RKIHL
Sbjct: 61  I-----PGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHL 120

Query: 211 FDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICY 270
           FDID+PGKITF ESKT++ G + +  DT   R+G+GICYD+RF ELA +YA RG  L+ Y
Sbjct: 121 FDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVY 180

Query: 271 PGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATT 330
           PGAFN+TTGP HWELLQRSRAVDNQ+YVAT SPAR+  ASYVAWGHST+V P+GEVLA  
Sbjct: 181 PGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKA 240

Query: 331 EHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLY 365
             +EAIV S+ID   +   R  +P+ +Q+R DLY
Sbjct: 241 GTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLY 269

[Close]

BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot
Match: Q6IR61 (Omega-amidase NIT2-A OS=Xenopus laevis OX=8355 GN=nit2a PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-80
Identity = 146/276 (52.90%), Postives = 193/276 (69.93%), Query Frame = 0


Query: 91  VTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAED 150
           + KFK++L Q  V+  K  N+  A K I+EA +KGAQ+V LPE +N PY     P YAE 
Sbjct: 1   MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60

Query: 151 IEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHL 210
           I        ST +LS+V++   + ++GGSI E    K+YN+  VFG DG L  KHRKIHL
Sbjct: 61  I-----PGESTELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIHL 120

Query: 211 FDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICY 270
           FDID+PGKI F ES+T++ G + +V DT   ++G+GICYDIRF ELA +YA +G  L+ Y
Sbjct: 121 FDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLVY 180

Query: 271 PGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATT 330
           PGAFNMTTGP HWELLQR+RA+DNQ+YVAT SPAR+  ASYVAWGHST+V P+GEV+A  
Sbjct: 181 PGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAKA 240

Query: 331 EHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQL 367
             +E ++ +EID  ++   R  +P+ +QRR DLY +
Sbjct: 241 GSEETVLSAEIDLQYLAEIREQIPIRRQRRRDLYNV 271

[Close]

BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot
Match: Q497B0 (Omega-amidase NIT2 OS=Rattus norvegicus OX=10116 GN=Nit2 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.1e-80
Identity = 148/276 (53.62%), Postives = 191/276 (69.20%), Query Frame = 0


Query: 91  VTKFKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAED 150
           ++ F++AL QL V++ K  NI  A   + EA ++GA +V LPE +N PY  +  P YAE 
Sbjct: 1   MSTFRLALIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEK 60

Query: 151 IEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHL 210
           I        ST  LSEV++   + ++GGSI E    K+YN+  VFG DG L  KHRKIHL
Sbjct: 61  I-----PGESTKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHL 120

Query: 211 FDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICY 270
           FDID+PGKITF ESKT++ G + +  DT   R+G+GICYD+RF ELA +YA RG  L+ Y
Sbjct: 121 FDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVY 180

Query: 271 PGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATT 330
           PGAFNMTTGP HWELLQR+RAVDNQ+YVAT SPAR+  ASYVAWGHST+V P+G+VL   
Sbjct: 181 PGAFNMTTGPAHWELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKA 240

Query: 331 EHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQL 367
             +E I+ S+ID   +   R  +P+LKQ+R DLY +
Sbjct: 241 GTEETILYSDIDLKKLSEIRQQIPILKQKRADLYSV 271

[Close]

BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot
Match: Q2T9R6 (Omega-amidase NIT2 OS=Bos taurus OX=9913 GN=NIT2 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.6e-80
Identity = 149/273 (54.58%), Postives = 192/273 (70.33%), Query Frame = 0


Query: 94  FKIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEA 153
           F++AL QL V++ K  N+  A   I EA ++GAQ+V LPE +N PY     P YAE I  
Sbjct: 4   FRLALIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKIP- 63

Query: 154 GGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDI 213
            GD   ST  LSEV++   + ++GGSI E+   K+YN+  VFG DG L  KHRK+HLFDI
Sbjct: 64  -GD---STQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDI 123

Query: 214 DIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYPGA 273
           D+PGKITF ES+T++ G + ++ DT   R+G+GICYDIRF ELA +YA RG  L+ YPGA
Sbjct: 124 DVPGKITFQESETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGA 183

Query: 274 FNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHD 333
           FN+TTGP HWELLQR RAVDNQ+YVAT SPAR+  ASYVAWGHST+V P+GEVLA    +
Sbjct: 184 FNLTTGPAHWELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTE 243

Query: 334 EAIVISEIDYSFIELRRTNLPLLKQRRGDLYQL 367
           E IV ++ID   +   R  +P+  Q+R DLY++
Sbjct: 244 ETIVYADIDLKKLAEIRQQIPIFSQKRSDLYEV 271

[Close]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL
Match: A0A0A0L5D9 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646400 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.3e-210
Identity = 374/375 (99.73%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSST GGGGGANLTTLRHKFRF
Sbjct: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSST-GGGGGANLTTLRHKFRF 60

Query: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120
           HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE
Sbjct: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120

Query: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180
           AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI
Sbjct: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180

Query: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240
           VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV
Sbjct: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240

Query: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300
           GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT
Sbjct: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300

Query: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360
           CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR
Sbjct: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360

Query: 361 GDLYQLVDAQRLKSE 376
           GDLYQLVDAQRLKSE
Sbjct: 361 GDLYQLVDAQRLKSE 374

[Close]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL
Match: A0A1S3BUK3 (omega-amidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493689 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 364/377 (96.55%), Postives = 367/377 (97.35%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300

Query: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360
           ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ
Sbjct: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360

Query: 361 RRGDLYQLVDAQRLKSE 376
           RRGDLYQLVD QRLKSE
Sbjct: 361 RRGDLYQLVDVQRLKSE 377

[Close]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL
Match: A0A5D3D924 (Omega-amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001870 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.8e-203
Identity = 361/375 (96.27%), Postives = 365/375 (97.33%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300

Query: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360
           ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ
Sbjct: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360

Query: 361 RRGDLYQLVDAQRLK 374
           RRGDLYQLVD QRL+
Sbjct: 361 RRGDLYQLVDVQRLQ 375

[Close]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL
Match: A0A5A7VDY8 (Omega-amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002440 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.3e-197
Identity = 358/389 (92.03%), Postives = 362/389 (93.06%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRS--------- 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQ+S         
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQQSFHLVCEQNN 300

Query: 301 ---RAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI 360
                   QLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI
Sbjct: 301 LTFNTFVVQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI 360

Query: 361 ELRRTNLPLLKQRRGDLYQLVDAQRLKSE 376
           ELRRTNLPLLKQRRGDLYQLVD QRLKSE
Sbjct: 361 ELRRTNLPLLKQRRGDLYQLVDVQRLKSE 389

[Close]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL
Match: A0A6J1FPQ5 (omega-amidase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445858 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.4e-183
Identity = 325/375 (86.67%), Postives = 343/375 (91.47%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MK+AISSLISSRNFNLH+SS S SIF +PSLLLPS+FPV ST     GAN T+LR K RF
Sbjct: 1   MKSAISSLISSRNFNLHRSSLSFSIFRRPSLLLPSQFPVISTDDKATGANFTSLRRKCRF 60

Query: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120
           HS KSS  MASSFNPEQARSPPA+PLPIPPVTKFKI LCQL VT DKQRNIEHARKAIEE
Sbjct: 61  HSTKSSPIMASSFNPEQARSPPAVPLPIPPVTKFKIGLCQLTVTPDKQRNIEHARKAIEE 120

Query: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180
           AVEKGAQLVVLPEIWN PYS D  P+YAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI
Sbjct: 121 AVEKGAQLVVLPEIWNGPYSNDSFPVYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180

Query: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240
            ERSGDK+YN+ C+FGTDGKLKAKHRKIHLFDIDIPGKITFIESKT+ AGQTPTVVDTEV
Sbjct: 181 AERSGDKLYNACCIFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTLTAGQTPTVVDTEV 240

Query: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300
           GRIGIGICYDIRF ELAM+YAARGAHLICYPGAFNMTTGPLHWELLQR+RA DNQLYVAT
Sbjct: 241 GRIGIGICYDIRFQELAMIYAARGAHLICYPGAFNMTTGPLHWELLQRARAADNQLYVAT 300

Query: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360
           CSPAR+  A YVAWGHSTLVGPFGEVLATTEHDEAI+I+EIDYS IELRRTNLPLLKQ+R
Sbjct: 301 CSPARDVGAGYVAWGHSTLVGPFGEVLATTEHDEAIIIAEIDYSLIELRRTNLPLLKQKR 360

Query: 361 GDLYQLVDAQRLKSE 376
           GDLYQLVD +RLKSE
Sbjct: 361 GDLYQLVDVERLKSE 375

[Close]

BLAST of CSPI04G25640 vs. NCBI nr
Match: XP_004141338.1 (omega-amidase, chloroplastic [Cucumis sativus] >KGN55366.1 hypothetical protein Csa_012020 [Cucumis sativus])

HSP 1 Score: 741.9 bits (1914), Expect = 2.7e-210
Identity = 374/375 (99.73%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSST GGGGGANLTTLRHKFRF
Sbjct: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSST-GGGGGANLTTLRHKFRF 60

Query: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120
           HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE
Sbjct: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120

Query: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180
           AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI
Sbjct: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180

Query: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240
           VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV
Sbjct: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240

Query: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300
           GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT
Sbjct: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300

Query: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360
           CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR
Sbjct: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360

Query: 361 GDLYQLVDAQRLKSE 376
           GDLYQLVDAQRLKSE
Sbjct: 361 GDLYQLVDAQRLKSE 374

[Close]

BLAST of CSPI04G25640 vs. NCBI nr
Match: XP_008452770.2 (PREDICTED: omega-amidase, chloroplastic [Cucumis melo])

HSP 1 Score: 721.5 bits (1861), Expect = 3.8e-204
Identity = 364/377 (96.55%), Postives = 367/377 (97.35%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300

Query: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360
           ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ
Sbjct: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360

Query: 361 RRGDLYQLVDAQRLKSE 376
           RRGDLYQLVD QRLKSE
Sbjct: 361 RRGDLYQLVDVQRLKSE 377

[Close]

BLAST of CSPI04G25640 vs. NCBI nr
Match: TYK20064.1 (omega-amidase [Cucumis melo var. makuwa])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 361/375 (96.27%), Postives = 365/375 (97.33%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYV 300

Query: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360
           ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ
Sbjct: 301 ATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQ 360

Query: 361 RRGDLYQLVDAQRLK 374
           RRGDLYQLVD QRL+
Sbjct: 361 RRGDLYQLVDVQRLQ 375

[Close]

BLAST of CSPI04G25640 vs. NCBI nr
Match: KAA0064526.1 (omega-amidase [Cucumis melo var. makuwa])

HSP 1 Score: 698.7 bits (1802), Expect = 2.7e-197
Identity = 358/389 (92.03%), Postives = 362/389 (93.06%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGG--ANLTTLRHKF 60
           MKAAISSLISSRNFNLHQSS SRSIF QPSLLLPSRFPVSSTGGGGGG  AN  TL HKF
Sbjct: 1   MKAAISSLISSRNFNLHQSSLSRSIFRQPSLLLPSRFPVSSTGGGGGGGDANFITLHHKF 60

Query: 61  RFHSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAI 120
           RFHSIKSSASMASSFNPEQARSPPALPL IPP+TKFKIALCQLAVTADKQRNIEHARKAI
Sbjct: 61  RFHSIKSSASMASSFNPEQARSPPALPLSIPPLTKFKIALCQLAVTADKQRNIEHARKAI 120

Query: 121 EEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180
           EEAVEKGAQLVVLPEIWNCPYS DCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG
Sbjct: 121 EEAVEKGAQLVVLPEIWNCPYSHDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGG 180

Query: 181 SIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDT 240
           SIVERSGDK+YNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAG+TPTVVDT
Sbjct: 181 SIVERSGDKLYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGETPTVVDT 240

Query: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRS--------- 300
           EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQ+S         
Sbjct: 241 EVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQQSFHLVCEQNN 300

Query: 301 ---RAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI 360
                   QLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI
Sbjct: 301 LTFNTFVVQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFI 360

Query: 361 ELRRTNLPLLKQRRGDLYQLVDAQRLKSE 376
           ELRRTNLPLLKQRRGDLYQLVD QRLKSE
Sbjct: 361 ELRRTNLPLLKQRRGDLYQLVDVQRLKSE 389

[Close]

BLAST of CSPI04G25640 vs. NCBI nr
Match: XP_038898898.1 (omega-amidase, chloroplastic [Benincasa hispida])

HSP 1 Score: 683.7 bits (1763), Expect = 8.9e-193
Identity = 349/375 (93.07%), Postives = 354/375 (94.40%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MKAAISSLISSRNFNLHQ S SRSIF QPSLLLPS FPV  T     GANL  + HKFRF
Sbjct: 1   MKAAISSLISSRNFNLHQFSLSRSIFRQPSLLLPSHFPVIFT-----GANL--ISHKFRF 60

Query: 61  HSIKSSASMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120
           HSIKSS  MASSF+PEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE
Sbjct: 61  HSIKSSPIMASSFSPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHARKAIEE 120

Query: 121 AVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSI 180
           AVEKGAQLVVLPEIWNCPYS D LPLYAEDIEAGGDASPSTAMLSEVSRRLKVTI+GGSI
Sbjct: 121 AVEKGAQLVVLPEIWNCPYSHDSLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIIGGSI 180

Query: 181 VERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240
           VERSGDK YN+SCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV
Sbjct: 181 VERSGDKFYNASCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPTVVDTEV 240

Query: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDNQLYVAT 300
           GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQR+RAVDNQLYVAT
Sbjct: 241 GRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRARAVDNQLYVAT 300

Query: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360
           CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR
Sbjct: 301 CSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLPLLKQRR 360

Query: 361 GDLYQLVDAQRLKSE 376
           GDLYQLVD Q LKSE
Sbjct: 361 GDLYQLVDVQGLKSE 368

[Close]

BLAST of CSPI04G25640 vs. TAIR 10
Match: AT5G12040.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 525.8 bits (1353), Expect = 2.9e-149
Identity = 265/381 (69.55%), Postives = 306/381 (80.31%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MK+AISS +   + NL   +         S  LP   P S T             H  + 
Sbjct: 1   MKSAISSSLFFNSKNLLNPNPLSRFISLKSNFLPKLSPRSITS------------HTLKL 60

Query: 61  HSIKSSA------SMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHA 120
            S  +SA      SMASSFNPEQAR P ALPLP PP+TKF I LCQL+VT+DK+RNI HA
Sbjct: 61  PSSSTSALRSISSSMASSFNPEQARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHA 120

Query: 121 RKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVT 180
           +KAIEEA  KGA+LV+LPEIWN PYS D  P+YAE+I+AGGDASPSTAMLSEVS+RLK+T
Sbjct: 121 KKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKIT 180

Query: 181 IVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPT 240
           I+GGSI ER GD++YN+ CVFG+DG+LKAKHRKIHLFDIDIPGKITF+ESKT+ AG+TPT
Sbjct: 181 IIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPT 240

Query: 241 VVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDN 300
           +VDT+VGRIGIGICYDIRF ELAM+YAARGAHL+CYPGAFNMTTGPLHWELLQR+RA DN
Sbjct: 241 IVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDN 300

Query: 301 QLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSFIELRRTNLP 360
           QLYVATCSPAR++ A Y AWGHSTLVGPFGEVLATTEH+EAI+I+EIDYS +E RRT+LP
Sbjct: 301 QLYVATCSPARDSGAGYTAWGHSTLVGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLP 360

Query: 361 LLKQRRGDLYQLVDAQRLKSE 376
           L +QRRGDLYQLVD QRL S+
Sbjct: 361 LNRQRRGDLYQLVDVQRLDSK 369

[Close]

BLAST of CSPI04G25640 vs. TAIR 10
Match: AT5G12040.2 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 202/303 (66.67%), Postives = 236/303 (77.89%), Query Frame = 0


Query: 1   MKAAISSLISSRNFNLHQSSFSRSIFHQPSLLLPSRFPVSSTGGGGGGANLTTLRHKFRF 60
           MK+AISS +   + NL   +         S  LP   P S T             H  + 
Sbjct: 1   MKSAISSSLFFNSKNLLNPNPLSRFISLKSNFLPKLSPRSITS------------HTLKL 60

Query: 61  HSIKSSA------SMASSFNPEQARSPPALPLPIPPVTKFKIALCQLAVTADKQRNIEHA 120
            S  +SA      SMASSFNPEQAR P ALPLP PP+TKF I LCQL+VT+DK+RNI HA
Sbjct: 61  PSSSTSALRSISSSMASSFNPEQARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNISHA 120

Query: 121 RKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVT 180
           +KAIEEA  KGA+LV+LPEIWN PYS D  P+YAE+I+AGGDASPSTAMLSEVS+RLK+T
Sbjct: 121 KKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKIT 180

Query: 181 IVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFDIDIPGKITFIESKTIAAGQTPT 240
           I+GGSI ER GD++YN+ CVFG+DG+LKAKHRKIHLFDIDIPGKITF+ESKT+ AG+TPT
Sbjct: 181 IIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGETPT 240

Query: 241 VVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYPGAFNMTTGPLHWELLQRSRAVDN 298
           +VDT+VGRIGIGICYDIRF ELAM+YAARGAHL+CYPGAFNMTTGPLHWELLQR+RA DN
Sbjct: 241 IVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDN 291

[Close]

BLAST of CSPI04G25640 vs. TAIR 10
Match: AT4G08790.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-31
Identity = 81/273 (29.67%), Postives = 140/273 (51.28%), Query Frame = 0


Query: 95  KIALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAG 154
           ++A  Q+    D   N     + ++EA   GA+L+  PE  N  +  D      ++ E+ 
Sbjct: 38  RVAAAQMTSVNDLMTNFATCSRLVQEAALAGAKLICFPE--NFSFVGD------KEGESV 97

Query: 155 GDASPSTAMLSE----VSRRLKVTIVGGSIVERSGD-KIYNSSCVFGTDGKLKAKHRKIH 214
             A P    + E    ++R   + +  G   ER  D  + N+  V    G ++  ++K+H
Sbjct: 98  KIAEPLDGPVMERYCSLARDSNIWLSLGGFQERFDDTHLCNTHVVIDDAGMIRDTYQKMH 157

Query: 215 LFDIDIPGKITFIESKTIAAGQTPTVVDTEVGRIGIGICYDIRFPEL-AMMYAARGAHLI 274
           LFD+D+PG  ++ ES     G     VD+ VGR+G+ +CYD+RFP++   +   + A ++
Sbjct: 158 LFDVDVPGGSSYKESSFTVPGTKIVSVDSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVL 217

Query: 275 CYPGAFNMTTGPLHWELLQRSRAVDNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLA 334
             P AF   TG  HWE+L R+RA++ Q YV   + A   +    ++G + ++ P+G V+ 
Sbjct: 218 LVPSAFTKVTGEAHWEILLRARAIETQCYVIAAAQAGKHNEKRESYGDTLIIDPWGTVVG 277

Query: 335 TTEH--DEAIVISEIDYSFIELRRTNLPLLKQR 360
                    IV+++ID+S I+  RT +P+ KQR
Sbjct: 278 RLPDRVSTGIVVADIDFSLIDSVRTKMPIDKQR 302

[Close]

BLAST of CSPI04G25640 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-17
Identity = 70/290 (24.14%), Postives = 126/290 (43.45%), Query Frame = 0


Query: 96  IALCQLAVTADKQRNIEHARKAIEEAVEKGAQLVVLPEIWNCPYSPDCLPLYAEDIEAGG 155
           ++  Q A + D   N+  A + + EA  KGA ++++ E++   Y         ED     
Sbjct: 12  VSSLQFACSDDISTNVAAAERLVREAHAKGANIILIQELFEGYY---FCQAQREDFFKRA 71

Query: 156 DA---SPSTAMLSEVSRRLKVTIVGGSIVERSGDKIYNSSCVFGTDGKLKAKHRKIHLFD 215
                 P+ A + ++++ L V ++  S  E +    YNS  +   DG     +RK H   
Sbjct: 72  KPYKNHPTIARMQKLAKELGV-VIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSH--- 131

Query: 216 IDIPGKITFIESKTIAAGQTP-TVVDTEVGRIGIGICYDIRFPELAMMYAARGAHLICYP 275
             IP    + E      G T   V  T+  +IG+ IC+D  FPE A     +GA ++ YP
Sbjct: 132 --IPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYP 191

Query: 276 GAFNMTTGPL------HWELLQRSRAVDNQLYVATCS-------PARNADASYVAWGHST 335
            A              HW  + +  A  N + +   +          +  +    +G S 
Sbjct: 192 TAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIGKEIIETEHGPSQITFYGTSF 251

Query: 336 LVGPFGEVLA-TTEHDEAIVISEIDYSFIELRRTNLPLLKQRRGDLYQLV 368
           + GP GE++A   +  EA+++++ D   I+ +R +  + + RR DLY+++
Sbjct: 252 IAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQSWGVFRDRRPDLYKVL 292

[Close]

BLAST of CSPI04G25640 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 82.8 bits (203), Expect = 6.5e-16
Identity = 79/291 (27.15%), Postives = 123/291 (42.27%), Query Frame = 0


Query: 88  IPPVTKFKIALCQLA-VTADKQRNIEHARKAIEEAVEKGAQLVVLPE------------- 147
           + P T  ++ + Q + V  D    I+ A K I EA  KGA+LV+ PE             
Sbjct: 19  VAPSTTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFG 78

Query: 148 ----IWNCPYSPDCLPLYAEDIEAGGDASPSTAMLSEVSRRLKVTIVGGSIVERSGDKIY 207
               + N     +    +A  I   G   P  A L++V+R+  V +V G+I E+ G  +Y
Sbjct: 79  LAVGVHNEEGRDEFRKYHASAIHVPG---PEVARLADVARKNHVYLVMGAI-EKEGYTLY 138

Query: 208 NSSCVFGTDGKLKAKHRKIHLFDID--IPGKITFIESKTIAAGQTPTVVDTEVGRIGIGI 267
            +   F   G+   KHRK+    ++  I G+           G T  V DT +G++G  I
Sbjct: 139 CTVLFFSPQGQFLGKHRKLMPTSLERCIWGQ---------GDGSTIPVYDTPIGKLGAAI 198

Query: 268 CYDIRFPELAMMYAARGAHLICYP---GAFNMTTGPLH------------WELLQRSRAV 327
           C++ R P       A+G  L C P   G+    +  LH             +  QR    
Sbjct: 199 CWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFP 258

Query: 328 DNQLYVATCSPARNADASYVAWGHSTLVGPFGEVLATTEHD-EAIVISEID 343
           D+  Y+ T         S V+ G S ++ P G+VLA    + E +V ++ID
Sbjct: 259 DHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAGPNFESEGLVTADID 296

The following BLAST results are available for this feature:

BLAST of CSPI04G25640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RUF8	4.1e-148	69.55	Omega-amidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NLP3 PE=1 SV=1	[more]
Q9NQR4	7.3e-81	54.74	Omega-amidase NIT2 OS=Homo sapiens OX=9606 GN=NIT2 PE=1 SV=1	[more]
Q6IR61	1.2e-80	52.90	Omega-amidase NIT2-A OS=Xenopus laevis OX=8355 GN=nit2a PE=2 SV=1	[more]
Q497B0	2.1e-80	53.62	Omega-amidase NIT2 OS=Rattus norvegicus OX=10116 GN=Nit2 PE=1 SV=1	[more]
Q2T9R6	3.6e-80	54.58	Omega-amidase NIT2 OS=Bos taurus OX=9913 GN=NIT2 PE=2 SV=1	[more]

BLAST of CSPI04G25640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5D9	1.3e-210	99.73	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G64640...	[more]
A0A1S3BUK3	1.9e-204	96.55	omega-amidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493689 PE=3 SV=1	[more]
A0A5D3D924	7.8e-203	96.27	Omega-amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001870...	[more]
A0A5A7VDY8	1.3e-197	92.03	Omega-amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002440...	[more]
A0A6J1FPQ5	2.4e-183	86.67	omega-amidase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445858 PE=4 ...	[more]

BLAST of CSPI04G25640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141338.1	2.7e-210	99.73	omega-amidase, chloroplastic [Cucumis sativus] >KGN55366.1 hypothetical protein ...	[more]
XP_008452770.2	3.8e-204	96.55	PREDICTED: omega-amidase, chloroplastic [Cucumis melo]	[more]
TYK20064.1	1.6e-202	96.27	omega-amidase [Cucumis melo var. makuwa]	[more]
KAA0064526.1	2.7e-197	92.03	omega-amidase [Cucumis melo var. makuwa]	[more]
XP_038898898.1	8.9e-193	93.07	omega-amidase, chloroplastic [Benincasa hispida]	[more]

BLAST of CSPI04G25640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G12040.1	2.9e-149	69.55	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT5G12040.2	1.5e-110	66.67	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT4G08790.1	1.4e-31	29.67	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein	[more]
AT2G27450.1	1.2e-17	24.14	nitrilase-like protein 1	[more]
AT3G44310.1	6.5e-16	27.15	nitrilase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 104..124
None	No IPR available	PANTHER	PTHR23088:SF54	OMEGA-AMIDASE, CHLOROPLASTIC-LIKE	coord: 58..374
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 58..374
None	No IPR available	CDD	cd07572	nit	coord: 95..360 e-value: 2.00891E-149 score: 420.68
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 95..347 e-value: 3.3E-62 score: 210.2
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 94..343 score: 64.065445
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 91..369 e-value: 1.3E-95 score: 321.6
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 92..367
IPR001110	Uncharacterised protein family UPF0012, conserved site	PROSITE	PS01227	UPF0012	coord: 244..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G25640.1	CSPI04G25640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006528	asparagine metabolic process
biological_process	GO:0006541	glutamine metabolic process
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006107	oxaloacetate metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0050152	omega-amidase activity