CSPI04G25620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G25620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RWP-RK domain-containing protein
Location	Chr4: 22942533 .. 22943757 (-)
RNA-Seq Expression	CSPI04G25620
Synteny	CSPI04G25620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGAGGAGTACTCAGCTCTTCCAGAGCCATTATCTGATTTTTATGATATTTTGTGCATCGGATTGGATTGGAATGACGATGAAGCCCCTCACATATCCCACCTTCAACCACTCTCGTCTTATCCAGGTTCATCTTTATTCTTACTTTTTTTATACTTCTTTTTTAAATGACTTTTTAAGTAATTAAAAATATAACCTCATAAAATTGTCGTAATATGTTCTTGTACTCGTTTTGTTTCCAGTGTATATTTGTTAAAATTTATTATTTTCGAGTTTCAAAATTTATGTAGGTTTTTGGAATGTTTTTTTAGTGGAATTTGAGTTAATAAACCAACCTAACTATTCTTCCTTTTACTTCTACTAAAAGCACTTGAAAAGTTATCACAAAAACATTATAGATTCCAAAATGTATACTATAATGTATATTTTTGTATAACCAATTTTGTCGCACACGCACACACACAAATCAAGATATTGAAATAAAAGTACTTAAATGGGAACTAACTATTACACGTGTCAAATTGCTATTCGATATGGTGTGACAAGTATTAAGCATATCTCTTCGGAACTCAAACTTGGCTTACTTGAGTTGCAGAGGGTGGAGAGGAATCCAATGGAGAAGAAGAGAGAGAAGAACAACAAATCCAAATGGAGAAACAATCACATCCACCGCCGGAAGTCGGGGGTAGTCGGCGAGTTCCCCAAACACCTCTAGCAAGACAGGTCTGTAAAAAGGAAAAAAAATATATCACATCATGGTTGTTCATAAAAGCAGATTAAAGCAGAGTGACTCTGTTTTTCGTTTTCCTTTTTCAAATCTCAATCGGAACAGAGACAGAGAGCTGGAAGAATGACGGTGAACGATTTGAAAGAATATCTTCACCTTCCAATCAGCGAGGCTGCGAAGAAGATGAACCTTTGTCTCACAGTCGTCAAGAAAATTTGCCGCCGGAGTGGACTCCGCCGCTGGCCTTACAGAAAGGTCCTTTTTTTTTTCTTTTCTCTTTCTTCTATTTTTATCCCCCAAATGTTTTCGTTATTTACGGGGTTTCCATGGAAAATTGAAATGGGTTATTAGGTGAAGAGTTATCAGAGGAAAATGGGAGCTTTAGGGACGAGACTGAGATCGAGAGATGCAGGGACGAGGGCCAGAGCTGAGGCTGAAATGGAAAGGCTACGCCAAGAATTGGCACAGTTTTGTGCTGGAATTGTACCTGATTAA

mRNA sequence

ATGGTTGAGGAGTACTCAGCTCTTCCAGAGCCATTATCTGATTTTTATGATATTTTGTGCATCGGATTGGATTGGAATGACGATGAAGCCCCTCACATATCCCACCTTCAACCACTCTCGTCTTATCCAGAGGGTGGAGAGGAATCCAATGGAGAAGAAGAGAGAGAAGAACAACAAATCCAAATGGAGAAACAATCACATCCACCGCCGGAAGTCGGGGGTAGTCGGCGAGTTCCCCAAACACCTCTAGCAAGACAGAGACAGAGAGCTGGAAGAATGACGGTGAACGATTTGAAAGAATATCTTCACCTTCCAATCAGCGAGGCTGCGAAGAAGATGAACCTTTGTCTCACAGTCGTCAAGAAAATTTGCCGCCGGAGTGGACTCCGCCGCTGGCCTTACAGAAAGGTGAAGAGTTATCAGAGGAAAATGGGAGCTTTAGGGACGAGACTGAGATCGAGAGATGCAGGGACGAGGGCCAGAGCTGAGGCTGAAATGGAAAGGCTACGCCAAGAATTGGCACAGTTTTGTGCTGGAATTGTACCTGATTAA

Coding sequence (CDS)

ATGGTTGAGGAGTACTCAGCTCTTCCAGAGCCATTATCTGATTTTTATGATATTTTGTGCATCGGATTGGATTGGAATGACGATGAAGCCCCTCACATATCCCACCTTCAACCACTCTCGTCTTATCCAGAGGGTGGAGAGGAATCCAATGGAGAAGAAGAGAGAGAAGAACAACAAATCCAAATGGAGAAACAATCACATCCACCGCCGGAAGTCGGGGGTAGTCGGCGAGTTCCCCAAACACCTCTAGCAAGACAGAGACAGAGAGCTGGAAGAATGACGGTGAACGATTTGAAAGAATATCTTCACCTTCCAATCAGCGAGGCTGCGAAGAAGATGAACCTTTGTCTCACAGTCGTCAAGAAAATTTGCCGCCGGAGTGGACTCCGCCGCTGGCCTTACAGAAAGGTGAAGAGTTATCAGAGGAAAATGGGAGCTTTAGGGACGAGACTGAGATCGAGAGATGCAGGGACGAGGGCCAGAGCTGAGGCTGAAATGGAAAGGCTACGCCAAGAATTGGCACAGTTTTGTGCTGGAATTGTACCTGATTAA

Protein sequence

MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQIQMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGIVPD*

Homology

[Close]

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot
Match: Q9LVU8 (Protein RKD4 OS=Arabidopsis thaliana OX=3702 GN=RKD4 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.5e-07
Identity = 23/54 (42.59%), Postives = 42/54 (77.78%), Query Frame = 0


Query: 86  QRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKS 140
           +++R  ++ ++++K++   PI +AAK++N+ LTV+KK CR  G+ RWP+RK+KS
Sbjct: 141 KKKRQDKLEMSEIKQFFDRPIMKAAKELNVGLTVLKKRCRELGIYRWPHRKLKS 194

[Close]

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot
Match: Q9M1B0 (Protein NLP9 OS=Arabidopsis thaliana OX=3702 GN=NLP9 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.4e-06
Identity = 26/83 (31.33%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 71  EVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLR 130
           E+ G+RR+ +   + ++     +++N L++Y    + +AAK + +C T +K+ICR+ G+ 
Sbjct: 518 EISGARRLEKKKSSTEK----NVSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIM 577

Query: 131 RWPYRKVKSYQRKMGALGTRLRS 154
           RWP RK+    R +  + T L S
Sbjct: 578 RWPSRKINKVNRSLRKIQTVLDS 596

[Close]

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot
Match: Q9M9U9 (Protein RKD1 OS=Arabidopsis thaliana OX=3702 GN=RKD1 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.2e-05
Identity = 23/72 (31.94%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 101 YLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRA 160
           Y ++PI++AA+++N+ LT++KK CR  G++RWP+RK+ S Q+ +  +    +        
Sbjct: 136 YFYMPITQAARELNIGLTLLKKRCRELGIKRWPHRKLMSLQKLISNVKELEKMEGEENED 195

Query: 161 RAEAEMERLRQE 173
           +    +E+L +E
Sbjct: 196 KLRNALEKLEKE 207

[Close]

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot
Match: Q9FGD1 (Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.1e-04
Identity = 40/155 (25.81%), Postives = 74/155 (47.74%), Query Frame = 0


Query: 25  WNDDEAPHISHLQP----LSSYPEGGEESNGEEEREEQQIQMEKQSHPPPEVGGSRRVPQ 84
           WN++   + + ++P    +S     G+ +  +   E+     EK S         +R+  
Sbjct: 95  WNENNGNYNNQVEPNLDEISRTNTMGDPNMEQILHEDVNTMKEKTS--------QKRIIM 154

Query: 85  TPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKSY 144
               R+      M+   +K+Y ++PI++AAK++N+ +T++KK CR  G+ RWP+RK+ S 
Sbjct: 155 KRRYREDGVINNMSREMMKQYFYMPITKAAKELNIGVTLLKKRCRELGIPRWPHRKLTS- 214

Query: 145 QRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQ 176
              + AL   L+     T+ R      R   EL +
Sbjct: 215 ---LNALIANLKDLLGNTKGRTPKSKLRNALELLE 237

[Close]

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot
Match: O81791 (Protein RKD5 OS=Arabidopsis thaliana OX=3702 GN=RKD5 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.1e-04
Identity = 28/103 (27.18%), Postives = 49/103 (47.57%), Query Frame = 0


Query: 37  QPLSSYPEGGEESNGEEEREEQQIQMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVN 96
           Q L+  P+   ES      + +  + E       E      + +       +    +++ 
Sbjct: 186 QDLNCLPDSETESEESVNEKTEHSEFENDKTEQSESDAKTEILKKKKRTPSRHVAELSLE 245

Query: 97  DLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKS 140
           +L +Y  L I EA++ + + LTV+KK CR  G+ RWP+RK+KS
Sbjct: 246 ELSKYFDLTIVEASRNLKVGLTVLKKKCREFGIPRWPHRKIKS 288

[Close]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL
Match: A0A0A0L2U8 (RWP-RK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646380 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.7e-97
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI
Sbjct: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
           QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI
Sbjct: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180

Query: 181 VPD 184
           VPD
Sbjct: 181 VPD 183

[Close]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL
Match: A0A5D3D916 (Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001880 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.7e-90
Identity = 173/183 (94.54%), Postives = 177/183 (96.72%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDD+AP +SH QPLSSYPEGGEES+GEEEREE QI
Sbjct: 212 MVEEYSALPEPLSDFYDILCIGLDWNDDDAPLVSHPQPLSSYPEGGEESDGEEEREE-QI 271

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
           QME+QSHPPPE GG RRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 272 QMEQQSHPPPEFGGRRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 331

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDA TRARAEAEMERLRQELAQFCAGI
Sbjct: 332 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAETRARAEAEMERLRQELAQFCAGI 391

Query: 181 VPD 184
           VPD
Sbjct: 392 VPD 393

[Close]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL
Match: A0A5A7VF79 (Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002430 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.1e-64
Identity = 139/183 (75.96%), Postives = 144/183 (78.69%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDD+AP +SH QPLSSYP    +S          +
Sbjct: 212 MVEEYSALPEPLSDFYDILCIGLDWNDDDAPLVSHPQPLSSYPVKQSDS------VFPFL 271

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
             + QS                   QRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 272 FFKSQS------------------EQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 331

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDA TRARAEAEMERLRQELAQFCAGI
Sbjct: 332 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAETRARAEAEMERLRQELAQFCAGI 370

Query: 181 VPD 184
           VPD
Sbjct: 392 VPD 370

[Close]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL
Match: A0A6J1CQA6 (protein RKD1-like OS=Momordica charantia OX=3673 GN=LOC111013572 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 2.2e-49
Identity = 107/121 (88.43%), Postives = 111/121 (91.74%), Query Frame = 0


Query: 62  MEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVK 121
           + +Q  P PE  G RR+PQT LARQRQRAGRMTVNDLK+YLHLPISEAAKKMNLCLTVVK
Sbjct: 2   LPEQQDPAPE-SGRRRIPQTALARQRQRAGRMTVNDLKDYLHLPISEAAKKMNLCLTVVK 61

Query: 122 KICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGIV 181
           KICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDA TR RAEAEMERLRQELAQFCAGIV
Sbjct: 62  KICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAETRNRAEAEMERLRQELAQFCAGIV 121

Query: 182 P 183
           P
Sbjct: 122 P 121

[Close]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL
Match: A0A6J1BQU6 (protein RKD3-like OS=Momordica charantia OX=3673 GN=LOC111004772 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 8.6e-38
Identity = 92/184 (50.00%), Postives = 123/184 (66.85%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDD-EAPHISHLQPLSSYPEGGEESNGEEEREEQQ 60
           ++E+Y+ +P+P+S+FYD LC+G+DW ++        L P           N E+E  +Q 
Sbjct: 67  ILEDYNMIPDPMSNFYDALCVGIDWFENLNTDGFFQLSP----------DNSEDEDMDQ- 126

Query: 61  IQMEKQSHPPPEVGGS--RRVPQTP-LARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLC 120
                   PPPE       R P+ P LA QR R GRMTVND+ +YLHLPISEA+KK+N+C
Sbjct: 127 --------PPPEFQNEPPERPPRRPSLAAQRARTGRMTVNDVWQYLHLPISEASKKLNVC 186

Query: 121 LTVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQF 180
            TV+KKICRRSGL RWPYRK++SY+R++ AL   + S    TRARAEAE+ER+++ELA F
Sbjct: 187 NTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMNSSYGDTRARAEAEIERVQKELADF 231

[Close]

BLAST of CSPI04G25620 vs. NCBI nr
Match: KGN55364.1 (hypothetical protein Csa_012951 [Cucumis sativus])

HSP 1 Score: 365.2 bits (936), Expect = 3.4e-97
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI
Sbjct: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
           QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI
Sbjct: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180

Query: 181 VPD 184
           VPD
Sbjct: 181 VPD 183

[Close]

BLAST of CSPI04G25620 vs. NCBI nr
Match: TYK20065.1 (protein RKD2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 173/183 (94.54%), Postives = 177/183 (96.72%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDD+AP +SH QPLSSYPEGGEES+GEEEREE QI
Sbjct: 212 MVEEYSALPEPLSDFYDILCIGLDWNDDDAPLVSHPQPLSSYPEGGEESDGEEEREE-QI 271

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
           QME+QSHPPPE GG RRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 272 QMEQQSHPPPEFGGRRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 331

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDA TRARAEAEMERLRQELAQFCAGI
Sbjct: 332 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAETRARAEAEMERLRQELAQFCAGI 391

Query: 181 VPD 184
           VPD
Sbjct: 392 VPD 393

[Close]

BLAST of CSPI04G25620 vs. NCBI nr
Match: KAG6608345.1 (Protein RKD4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 262.7 bits (670), Expect = 2.4e-66
Identity = 143/185 (77.30%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDE---APHISHLQPLSSYPEGGEESNGEEEREE 60
           M +EY+A+PEPLSDFYD+LCIGLDWND+    +PH ++ QPL   P   E  + EEERE+
Sbjct: 189 MFDEYTAIPEPLSDFYDVLCIGLDWNDESPLLSPH-NYSQPLPLDPV-AEREDDEEERED 248

Query: 61  QQIQMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL 120
            Q++M++Q    PE  G RRVPQT LARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL
Sbjct: 249 -QMEMDQQPDQSPEF-GRRRVPQTALARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL 308

Query: 121 TVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFC 180
           TVVKKICRRSGLRRWPYRKVKSYQRKMGALG RLRSRDA TRARAEAEM+RLRQELAQFC
Sbjct: 309 TVVKKICRRSGLRRWPYRKVKSYQRKMGALGMRLRSRDAETRARAEAEMDRLRQELAQFC 368

Query: 181 AGIVP 183
           AGIVP
Sbjct: 369 AGIVP 369

[Close]

BLAST of CSPI04G25620 vs. NCBI nr
Match: KAG7037698.1 (Protein RKD3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 259.6 bits (662), Expect = 2.0e-65
Identity = 141/184 (76.63%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDE---APHISHLQPLSSYPEGGEESNGEEEREE 60
           M +EY+A+PEPLSDFYD+LCIGLDWND+    +PH ++ QPL   P   E  + EEERE+
Sbjct: 187 MFDEYTAIPEPLSDFYDVLCIGLDWNDESPLLSPH-NYSQPLPLDPV-AEREDDEEERED 246

Query: 61  QQIQMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL 120
            Q++M++Q    PE  G RRVPQT LARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL
Sbjct: 247 -QMEMDQQPDQSPEF-GRRRVPQTALARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCL 306

Query: 121 TVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFC 180
           TVVKKICRRSGLRRWPYRKVKSYQRKMGALG RLRSRDA TRARAEAEM+RLRQELAQFC
Sbjct: 307 TVVKKICRRSGLRRWPYRKVKSYQRKMGALGMRLRSRDAETRARAEAEMDRLRQELAQFC 366

Query: 181 AGIV 182
           AGI+
Sbjct: 367 AGII 366

[Close]

BLAST of CSPI04G25620 vs. NCBI nr
Match: KAA0064525.1 (protein RKD2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 254.2 bits (648), Expect = 8.5e-64
Identity = 139/183 (75.96%), Postives = 144/183 (78.69%), Query Frame = 0


Query: 1   MVEEYSALPEPLSDFYDILCIGLDWNDDEAPHISHLQPLSSYPEGGEESNGEEEREEQQI 60
           MVEEYSALPEPLSDFYDILCIGLDWNDD+AP +SH QPLSSYP    +S          +
Sbjct: 212 MVEEYSALPEPLSDFYDILCIGLDWNDDDAPLVSHPQPLSSYPVKQSDS------VFPFL 271

Query: 61  QMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 120
             + QS                   QRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV
Sbjct: 272 FFKSQS------------------EQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVV 331

Query: 121 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRARAEAEMERLRQELAQFCAGI 180
           KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDA TRARAEAEMERLRQELAQFCAGI
Sbjct: 332 KKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAETRARAEAEMERLRQELAQFCAGI 370

Query: 181 VPD 184
           VPD
Sbjct: 392 VPD 370

[Close]

BLAST of CSPI04G25620 vs. TAIR 10
Match: AT5G53040.1 (RWP-RK domain-containing protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.2e-08
Identity = 23/54 (42.59%), Postives = 42/54 (77.78%), Query Frame = 0


Query: 86  QRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKS 140
           +++R  ++ ++++K++   PI +AAK++N+ LTV+KK CR  G+ RWP+RK+KS
Sbjct: 141 KKKRQDKLEMSEIKQFFDRPIMKAAKELNVGLTVLKKRCRELGIYRWPHRKLKS 194

[Close]

BLAST of CSPI04G25620 vs. TAIR 10
Match: AT3G59580.1 (Plant regulator RWP-RK family protein )

HSP 1 Score: 52.4 bits (124), Expect = 4.6e-07
Identity = 26/83 (31.33%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 71  EVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLR 130
           E+ G+RR+ +   + ++     +++N L++Y    + +AAK + +C T +K+ICR+ G+ 
Sbjct: 518 EISGARRLEKKKSSTEK----NVSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIM 577

Query: 131 RWPYRKVKSYQRKMGALGTRLRS 154
           RWP RK+    R +  + T L S
Sbjct: 578 RWPSRKINKVNRSLRKIQTVLDS 596

[Close]

BLAST of CSPI04G25620 vs. TAIR 10
Match: AT3G59580.2 (Plant regulator RWP-RK family protein )

HSP 1 Score: 52.4 bits (124), Expect = 4.6e-07
Identity = 26/83 (31.33%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 71  EVGGSRRVPQTPLARQRQRAGRMTVNDLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLR 130
           E+ G+RR+ +   + ++     +++N L++Y    + +AAK + +C T +K+ICR+ G+ 
Sbjct: 518 EISGARRLEKKKSSTEK----NVSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIM 577

Query: 131 RWPYRKVKSYQRKMGALGTRLRS 154
           RWP RK+    R +  + T L S
Sbjct: 578 RWPSRKINKVNRSLRKIQTVLDS 596

[Close]

BLAST of CSPI04G25620 vs. TAIR 10
Match: AT1G18790.1 (RWP-RK domain-containing protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.3e-06
Identity = 23/72 (31.94%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 101 YLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKSYQRKMGALGTRLRSRDAGTRA 160
           Y ++PI++AA+++N+ LT++KK CR  G++RWP+RK+ S Q+ +  +    +        
Sbjct: 136 YFYMPITQAARELNIGLTLLKKRCRELGIKRWPHRKLMSLQKLISNVKELEKMEGEENED 195

Query: 161 RAEAEMERLRQE 173
           +    +E+L +E
Sbjct: 196 KLRNALEKLEKE 207

[Close]

BLAST of CSPI04G25620 vs. TAIR 10
Match: AT4G35590.1 (RWP-RK domain-containing protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.5e-05
Identity = 28/103 (27.18%), Postives = 49/103 (47.57%), Query Frame = 0


Query: 37  QPLSSYPEGGEESNGEEEREEQQIQMEKQSHPPPEVGGSRRVPQTPLARQRQRAGRMTVN 96
           Q L+  P+   ES      + +  + E       E      + +       +    +++ 
Sbjct: 186 QDLNCLPDSETESEESVNEKTEHSEFENDKTEQSESDAKTEILKKKKRTPSRHVAELSLE 245

Query: 97  DLKEYLHLPISEAAKKMNLCLTVVKKICRRSGLRRWPYRKVKS 140
           +L +Y  L I EA++ + + LTV+KK CR  G+ RWP+RK+KS
Sbjct: 246 ELSKYFDLTIVEASRNLKVGLTVLKKKCREFGIPRWPHRKIKS 288

The following BLAST results are available for this feature:

BLAST of CSPI04G25620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVU8	4.5e-07	42.59	Protein RKD4 OS=Arabidopsis thaliana OX=3702 GN=RKD4 PE=3 SV=1	[more]
Q9M1B0	6.4e-06	31.33	Protein NLP9 OS=Arabidopsis thaliana OX=3702 GN=NLP9 PE=2 SV=1	[more]
Q9M9U9	3.2e-05	31.94	Protein RKD1 OS=Arabidopsis thaliana OX=3702 GN=RKD1 PE=3 SV=1	[more]
Q9FGD1	2.1e-04	25.81	Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1	[more]
O81791	2.1e-04	27.18	Protein RKD5 OS=Arabidopsis thaliana OX=3702 GN=RKD5 PE=3 SV=1	[more]

BLAST of CSPI04G25620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2U8	1.7e-97	100.00	RWP-RK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646380 PE=4...	[more]
A0A5D3D916	5.7e-90	94.54	Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A5A7VF79	4.1e-64	75.96	Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A6J1CQA6	2.2e-49	88.43	protein RKD1-like OS=Momordica charantia OX=3673 GN=LOC111013572 PE=4 SV=1	[more]
A0A6J1BQU6	8.6e-38	50.00	protein RKD3-like OS=Momordica charantia OX=3673 GN=LOC111004772 PE=4 SV=1	[more]

BLAST of CSPI04G25620 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN55364.1	3.4e-97	100.00	hypothetical protein Csa_012951 [Cucumis sativus]	[more]
TYK20065.1	1.2e-89	94.54	protein RKD2-like isoform X1 [Cucumis melo var. makuwa]	[more]
KAG6608345.1	2.4e-66	77.30	Protein RKD4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7037698.1	2.0e-65	76.63	Protein RKD3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAA0064525.1	8.5e-64	75.96	protein RKD2-like isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI04G25620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53040.1	3.2e-08	42.59	RWP-RK domain-containing protein	[more]
AT3G59580.1	4.6e-07	31.33	Plant regulator RWP-RK family protein	[more]
AT3G59580.2	4.6e-07	31.33	Plant regulator RWP-RK family protein	[more]
AT1G18790.1	2.3e-06	31.94	RWP-RK domain-containing protein	[more]
AT4G35590.1	1.5e-05	27.18	RWP-RK domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..179
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..91
None	No IPR available	PANTHER	PTHR46373:SF5	RWP-RK DOMAIN PROTEIN	coord: 3..179
IPR003035	RWP-RK domain	PFAM	PF02042	RWP-RK	coord: 93..139 e-value: 1.1E-19 score: 70.1
IPR003035	RWP-RK domain	PROSITE	PS51519	RWP_RK	coord: 80..160 score: 16.847216
IPR044607	Transcription factor RKD-like	PANTHER	PTHR46373	PROTEIN RKD4	coord: 3..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G25620.1	CSPI04G25620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity