CSPI04G25430 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G25430
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Protein of unknown function (DUF803)
Location	Chr4: 22839541 .. 22842262 (-)
RNA-Seq Expression	CSPI04G25430
Synteny	CSPI04G25430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGAACCGATTTGCTTAACTTTAGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTTCGCTTTCTCTTTTTCTTTCTCTTACCTTTGTTTTTTTTTTTTCACATTTTCTTCTAGGTCCTTTAATTTCATGCGATTCGTTTTCTTGTTCTTCCTTGAGCTGTCCGATCGCTTGCAGTTATGGTGTTTCTTTGATTCTCTAATTTTTCGGATTCCATTAAAGATTTGAGCGAATTTTATTTTCGCTTTCTTCTGGTGAATTCCTTGAATGAAGTTGTGAATTCTCGTTGGTTTTTTGTGTTGTATAGGTGATAAGGGCTTACGCTTTTAACAAAACGTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTAAGTTCTTTTATTCAGCTACATACGCCATTTTCTTTTATGTTTTTTGATTGGCATTCGATGATTGGATCTTAGGCATTATTGAGCGAATATGCTGTTTTTGTTCTGAGATCTAGATGAATTTAGCGCGCCTTTATAGTCTCCCTACAGGCTACATTTGATGGAAACTATCAATTGCTGTTGTGATGGCTAACTGTCTCCTCTTTTATTCAGCATCGCCTTTATGGTCGTATTTATTATATTGTATTTATCTCTATGAAAATTACATATAATCTACGATTCTAAATATTTCATAGATTTTTTTCCCAAGAATCCGTACCTCGCAGGAAAATTTGTATGTTGCGCACATTTTCGTCTCTAATATGCATTTCCTTTCTAATTTTTGAATTTCAGGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATGCTTGCTGGCATTGGCACCATAGGTAATGTAAGTTCATCTTTGTGTCTTTATTAGTTTAGTTATTGTTTGATCTCTATTTTATGCATTTCAGGTTTGGCCATGCCTCGTATGCCAAGTTTTTATTAATCTTCATCTTCCTTGCACACATTTGCAATTTACTTGGCTATTTTCGCCATTTCTAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTAGGTATCTTATATTATTATAACCCCACGTTTTATAACAATCTCTGGTCATTTATGTTAGAAGTTTTATGGACTTGCTTGAAGAAACAAATATAACATACTAGTACTTCGTAAATAAGGCTGTAAAAGATGAGTGAATTGACATCTAGAAATGATTGGCTACGCATATATTTTTAACAGGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGGTAAATTCATTGCTTTTATATTGACACTTCAATTGTTTTCTTTTCCTCCCAGAAAAATTATATGTTACATATGTATGTTGCAGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGTACTTTTCTTGTTTGCACTCTTTTACTTGATGGATTACATTTCCTTAAAATCATTAAGGTCGTAGAGGATGACTATTAGGAATTAGAACTTTGCTTCACTGTGAGGAATTGGAGCTTCTGCCTGACTGTGGTGGTATCCTAGTTGTTTCAAATTGGATCTTAGTCTGCTTTTATTTTGACCTTCAGAATGTACTAGGCATTGAGAAGTAGAATTTTGAATAATCGTTGTTGTGGTTTTCTGGAACATAAGCATACCATAGAATTGTTAGTTTAGTCTTTTACATATATGGCATAAATATAGAGTGGAACTAATCCAATTGGCCTTTTAGATTCGTAATAGCAAAATTCACAAGTTAAATCTGGAATTGCAGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTCATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGGTACTTGAAATGCTTGGTGATTTGATCTCTTTACGATTCTATTTTGCACTCAGTGTTAGTTGTTTTATCAACTTCTGTCTGTGTTCTTCTAGTTCATTGTCTTCTTTTTTAGTAGACTCATTGTCTTCTTTTTTAGTAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATTGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCATCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGTAAACTCTCCTACCTATAATTCCCTTTCGAGTTTACAATGACTGACCAGACTTGATAAAGAGTGGTTTCTGGCCATGGGAATGTGTTCATTCATCTTCCTCTGTACAGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACATTGCAAAATTTGTTGTCTTCAAAAGCAAAGGCTGACGCTTGA

mRNA sequence

ATGTGGGAACCGATTTGCTTAACTTTAGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGGGCTTACGCTTTTAACAAAACGTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATGCTTGCTGGCATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTCATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATTGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCATCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACATTGCAAAATTTGTTGTCTTCAAAAGCAAAGGCTGACGCTTGA

Coding sequence (CDS)

ATGTGGGAACCGATTTGCTTAACTTTAGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGGGCTTACGCTTTTAACAAAACGTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATGCTTGCTGGCATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTCATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATTGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCATCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACATTGCAAAATTTGTTGTCTTCAAAAGCAAAGGCTGACGCTTGA

Protein sequence

MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA*

Homology

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BLAST of CSPI04G25430 vs. ExPASy Swiss-Prot
Match: Q8RWH8 (Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g11960 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-141
Identity = 254/341 (74.49%), Postives = 299/341 (87.68%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MNV DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR L   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 342
           ++ ++R F  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

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BLAST of CSPI04G25430 vs. ExPASy Swiss-Prot
Match: P55580 (Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a02340 PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 6.0e-05
Identity = 37/117 (31.62%), Postives = 55/117 (47.01%), Query Frame = 0


Query: 4   PICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLML 63
           PI L  AA   + IG IL K G   LPP +          F    + G +      +L  
Sbjct: 5   PIILVFAAGLNSCIGNILLKWGRASLPPSAGLADTFLTPGF----VGGVVFYGINVLLFA 64

Query: 64  RALSLAPVSIIQPV-SGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGG 120
           +AL    VS+  P+ +G G A+L I SH++  E  ++  W+G+ L  +G I +  GG
Sbjct: 65  KALDSLEVSVAYPILAGSGFAMLIIASHYFFGEPFHLHKWIGVALVLVGIIFLARGG 117

[Close]

BLAST of CSPI04G25430 vs. ExPASy Swiss-Prot
Match: Q8BGN5 (NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-04
Identity = 65/268 (24.25%), Postives = 113/268 (42.16%), Query Frame = 0


Query: 40  RAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN- 99
           RAY   KTW +G L+ + G + +  + + AP+S+I P+S   +   +I    ++KE    
Sbjct: 72  RAYFKTKTWWLGLLLLLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKP 131

Query: 100 -------VVDWMGIMLAGIGT---IGVGAGGEEQKASAISVFHL---PWLAFIMT--ILF 159
                  V+ ++G  LA +GT   +       E+        HL   P+L +++   +LF
Sbjct: 132 KDFVRRYVLSFVGCGLAIVGTYLLVTFAPNSHEKMTGENIARHLVSWPFLLYMLVAIVLF 191

Query: 160 VLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQIL 219
            LL   L+FYK +          VV  ++  L   +       +S M  L + QG  Q+ 
Sbjct: 192 CLL---LYFYKERNANS-----IVVILLLVALLGSMTVVTVKAVSGMLVLSI-QGNLQLD 251

Query: 220 VPICIFISICCSATGFYYQTRGLKHGRAI----VVSTCAAVASIVTGVLAGMLALGEELP 279
            PI   + +C  AT   YQ   L     I    ++++   + S    + AG +   + L 
Sbjct: 252 YPIFYVMFVCMVATAI-YQATFLSEASQIYDSSLIASVGYILSTTAAITAGAIFYLDFLG 311

Query: 280 SSPTGRLFLLLGWLLIIIGVILLVSSSR 288
                     LG L+  +GV L+  + +
Sbjct: 312 EEALHICMFALGCLIAFLGVFLITRNRK 329

[Close]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL
Match: A0A0A0L5B6 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 8.0e-186
Identity = 343/344 (99.71%), Postives = 343/344 (99.71%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

[Close]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL
Match: A0A5A7VA96 (Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002250 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 340/344 (98.84%), Postives = 342/344 (99.42%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+GVDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

[Close]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL
Match: A0A1S3BUH9 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 340/344 (98.84%), Postives = 342/344 (99.42%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+GVDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

[Close]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL
Match: A0A6J1FDL9 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.8e-177
Identity = 327/345 (94.78%), Postives = 338/345 (97.97%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RRLTWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 345
           RSGVDR+FGHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

[Close]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL
Match: A0A6J1FKB2 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111446202 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.0e-177
Identity = 322/344 (93.60%), Postives = 335/344 (97.38%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGK+LQKKGTVILPPLSFKLKVIRAYAFN+ WIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKVLQKKGTVILPPLSFKLKVIRAYAFNRAWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN+VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNIVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKAS IS+F LPWLAF +TILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASEISIFRLPWLAFFITILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISK GFLFLEQGFH+ILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKTGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASI+TGV+AGMLALGEELPSSPT RLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIITGVVAGMLALGEELPSSPTARLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           +SG DRNFGHRSGS+VR+RDSSPSAIIQTTTLQNLLSSKAKAD+
Sbjct: 301 QSGTDRNFGHRSGSSVRIRDSSPSAIIQTTTLQNLLSSKAKADS 344

[Close]

BLAST of CSPI04G25430 vs. NCBI nr
Match: XP_004141325.1 (probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hypothetical protein Csa_012133 [Cucumis sativus])

HSP 1 Score: 659.4 bits (1700), Expect = 1.6e-185
Identity = 343/344 (99.71%), Postives = 343/344 (99.71%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

[Close]

BLAST of CSPI04G25430 vs. NCBI nr
Match: XP_008452740.1 (PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 putative magnesium transporter NIPA9 [Cucumis melo var. makuwa])

HSP 1 Score: 655.6 bits (1690), Expect = 2.4e-184
Identity = 340/344 (98.84%), Postives = 342/344 (99.42%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+GVDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

[Close]

BLAST of CSPI04G25430 vs. NCBI nr
Match: XP_038896971.1 (probable magnesium transporter NIPA9 [Benincasa hispida])

HSP 1 Score: 644.0 bits (1660), Expect = 7.1e-181
Identity = 333/345 (96.52%), Postives = 340/345 (98.55%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGA+
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAI 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILF+LLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFILLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASI+TGV+AGMLALGEELPSSPTGRL LLLGWLLII+GVILLVSSSRLIRR TWLYRRFK
Sbjct: 241 ASIITGVVAGMLALGEELPSSPTGRLLLLLGWLLIIVGVILLVSSSRLIRRFTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNL-LSSKAKADA 345
           RSGVDRNFGHRSGS+VRLRDSSPSAIIQTTTLQNL LSSK KADA
Sbjct: 301 RSGVDRNFGHRSGSSVRLRDSSPSAIIQTTTLQNLMLSSKEKADA 345

[Close]

BLAST of CSPI04G25430 vs. NCBI nr
Match: XP_031740158.1 (probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus])

HSP 1 Score: 642.9 bits (1657), Expect = 1.6e-180
Identity = 343/376 (91.22%), Postives = 343/376 (91.22%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTI------- 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTI       
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGNVWPCL 120

Query: 121 -------------------------GVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 180
                                    GVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG
Sbjct: 121 VCQVFINIHLPCTHLQFTWLFSPFLGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 180

Query: 181 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 240
           WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI
Sbjct: 181 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 240

Query: 241 FISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL 300
            ISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL
Sbjct: 241 LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL 300

Query: 301 LLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQ 345
           LLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQ
Sbjct: 301 LLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQ 360

[Close]

BLAST of CSPI04G25430 vs. NCBI nr
Match: XP_022936425.1 (probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probable magnesium transporter NIPA9 [Cucurbita pepo subsp. pepo] >KAG6591472.1 putative magnesium transporter NIPA9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 631.7 bits (1628), Expect = 3.7e-177
Identity = 327/345 (94.78%), Postives = 338/345 (97.97%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICI ISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RRLTWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 345
           RSGVDR+FGHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

[Close]

BLAST of CSPI04G25430 vs. TAIR 10
Match: AT5G11960.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 504.2 bits (1297), Expect = 8.2e-143
Identity = 254/341 (74.49%), Postives = 299/341 (87.68%), Query Frame = 0


Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MNV DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICIFISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR L   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 342
           ++ ++R F  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

The following BLAST results are available for this feature:

BLAST of CSPI04G25430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWH8	1.2e-141	74.49	Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g1196...	[more]
P55580	6.0e-05	31.62	Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR23...	[more]
Q8BGN5	3.0e-04	24.25	NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1	[more]

BLAST of CSPI04G25430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5B6	8.0e-186	99.71	Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 S...	[more]
A0A5A7VA96	1.1e-184	98.84	Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A1S3BUH9	1.1e-184	98.84	Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1	[more]
A0A6J1FDL9	1.8e-177	94.78	Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=...	[more]
A0A6J1FKB2	3.0e-177	93.60	Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111446202 PE=...	[more]

BLAST of CSPI04G25430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141325.1	1.6e-185	99.71	probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hy...	[more]
XP_008452740.1	2.4e-184	98.84	PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 put...	[more]
XP_038896971.1	7.1e-181	96.52	probable magnesium transporter NIPA9 [Benincasa hispida]	[more]
XP_031740158.1	1.6e-180	91.22	probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus]	[more]
XP_022936425.1	3.7e-177	94.78	probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probab...	[more]

BLAST of CSPI04G25430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11960.1	8.2e-143	74.49	Protein of unknown function (DUF803)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008521	Magnesium transporter NIPA	PFAM	PF05653	Mg_trans_NIPA	coord: 40..283 e-value: 1.6E-7 score: 30.7
IPR008521	Magnesium transporter NIPA	PANTHER	PTHR12570	UNCHARACTERIZED	coord: 1..341
None	No IPR available	GENE3D	1.10.3730.20		coord: 5..118 e-value: 1.5E-6 score: 30.4
None	No IPR available	PANTHER	PTHR12570:SF65	MAGNESIUM TRANSPORTER NIPA9-RELATED	coord: 1..341
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 25..119
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 190..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G25430.1	CSPI04G25430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1903830	magnesium ion transmembrane transport
biological_process	GO:0015693	magnesium ion transport
cellular_component	GO:0005769	early endosome
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015095	magnesium ion transmembrane transporter activity