CSPI04G25000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G25000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RNA-binding (RRM/RBD/RNP motifs) family protein
Location	Chr4: 22553227 .. 22554239 (+)
RNA-Seq Expression	CSPI04G25000
Synteny	CSPI04G25000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTGGAAGTTGAAAAGAAAAAAAAAAACCATACGTGATAAAATCTTTGGAGCCAACAAGGTTGAATATGAGTACTACTAGGATGGACAATAACAATGATCAGTGTAGGGGAAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAACTAACTGAGGAGGAGTTCAGAAGTTACTTTGAAAATTTTGGTCAGATAACTGATGTAGTTGTGATGCATGATAGTGTAACCAATCGCCCTAGAGGATTTGGATTTGTCACATTTGAATCTTTAGAATCTGTTGACTCAGTTCTGCAAAGGAACTTTCATGAACTGAATGGGAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGCAACTACAATGGTGTTCATATTCCCAATGCTAGAGCAGCAAAAAGTCCACTTCTTAATGGTAATAGGAATGTTCCTTTCCCTTACCCTTATTATGGTTCGGGTTATCCAATTTATCCCGGTTTTACACATTTTCCACATTATGGAGCAGCAGTTGATTCGTATGGTTCTAACTTCTATGGGAATTGGTTTCCCCCTGGTTCTGGTAACATAGCTTCAGATGGATGGTGGTATGGACCTAATTTTCTTAGTGTTGGGATGTTACCTCCACTTTGTAGAAATTCTTTAATGCATCCAAGCAAGGGGATCTATATAGGGAGCAGAAATGGAATGGCTAATACCAGAAACGGGGAGATAAAGCAGAACACGGCCGGAAAACGAATTATGTCGGCTGATGCAGCTCCATTTCAGATCAAAGACAGATCTTCTTGAAGATATTTTAACTTAAATATTTGTTATTGGATTTGCATCTTACGTAATGGCATTTGATTTGTAAATTCTGTTGGGATAGATCTATGTCTCTCATTCTTAGAATTTTTGGGATATAATTGTTCTGTGAATAAATCTCTCCTTTGGGGTATGACAATGCTTGTGAATAATTTTTGTGGACCAAGACAATGATTATGATTAATTGCTTATGGTGCACTGAAATATTGAAGGG

mRNA sequence

AATTGGAAGTTGAAAAGAAAAAAAAAAACCATACGTGATAAAATCTTTGGAGCCAACAAGGTTGAATATGAGTACTACTAGGATGGACAATAACAATGATCAGTGTAGGGGAAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAACTAACTGAGGAGGAGTTCAGAAGTTACTTTGAAAATTTTGGTCAGATAACTGATGTAGTTGTGATGCATGATAGTGTAACCAATCGCCCTAGAGGATTTGGATTTGTCACATTTGAATCTTTAGAATCTGTTGACTCAGTTCTGCAAAGGAACTTTCATGAACTGAATGGGAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGCAACTACAATGGTGTTCATATTCCCAATGCTAGAGCAGCAAAAAGTCCACTTCTTAATGGTAATAGGAATGTTCCTTTCCCTTACCCTTATTATGGTTCGGGTTATCCAATTTATCCCGGTTTTACACATTTTCCACATTATGGAGCAGCAGTTGATTCGTATGGTTCTAACTTCTATGGGAATTGGTTTCCCCCTGGTTCTGGTAACATAGCTTCAGATGGATGGTGGTATGGACCTAATTTTCTTAGTGTTGGGATGTTACCTCCACTTTGTAGAAATTCTTTAATGCATCCAAGCAAGGGGATCTATATAGGGAGCAGAAATGGAATGGCTAATACCAGAAACGGGGAGATAAAGCAGAACACGGCCGGAAAACGAATTATGTCGGCTGATGCAGCTCCATTTCAGATCAAAGACAGATCTTCTTGAAGATATTTTAACTTAAATATTTGTTATTGGATTTGCATCTTACGTAATGGCATTTGATTTGTAAATTCTGTTGGGATAGATCTATGTCTCTCATTCTTAGAATTTTTGGGATATAATTGTTCTGTGAATAAATCTCTCCTTTGGGGTATGACAATGCTTGTGAATAATTTTTGTGGACCAAGACAATGATTATGATTAATTGCTTATGGTGCACTGAAATATTGAAGGG

Coding sequence (CDS)

ATGAGTACTACTAGGATGGACAATAACAATGATCAGTGTAGGGGAAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAACTAACTGAGGAGGAGTTCAGAAGTTACTTTGAAAATTTTGGTCAGATAACTGATGTAGTTGTGATGCATGATAGTGTAACCAATCGCCCTAGAGGATTTGGATTTGTCACATTTGAATCTTTAGAATCTGTTGACTCAGTTCTGCAAAGGAACTTTCATGAACTGAATGGGAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGCAACTACAATGGTGTTCATATTCCCAATGCTAGAGCAGCAAAAAGTCCACTTCTTAATGGTAATAGGAATGTTCCTTTCCCTTACCCTTATTATGGTTCGGGTTATCCAATTTATCCCGGTTTTACACATTTTCCACATTATGGAGCAGCAGTTGATTCGTATGGTTCTAACTTCTATGGGAATTGGTTTCCCCCTGGTTCTGGTAACATAGCTTCAGATGGATGGTGGTATGGACCTAATTTTCTTAGTGTTGGGATGTTACCTCCACTTTGTAGAAATTCTTTAATGCATCCAAGCAAGGGGATCTATATAGGGAGCAGAAATGGAATGGCTAATACCAGAAACGGGGAGATAAAGCAGAACACGGCCGGAAAACGAATTATGTCGGCTGATGCAGCTCCATTTCAGATCAAAGACAGATCTTCTTGA

Protein sequence

MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSVGMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRSS*

Homology

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BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot
Match: Q9C652 (RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBP1 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.4e-31
Identity = 76/133 (57.14%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 6   MDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFE 65
           M +N    R +KIFVGGLS+  TEEEF+SYFE FG+ TDVVVMHD VTNRPRGFGFVT++
Sbjct: 110 MSSNGVTSRTKKIFVGGLSSNTTEEEFKSYFERFGRTTDVVVMHDGVTNRPRGFGFVTYD 169

Query: 66  SLESVDSVLQRNFHELNGRRVEVKRAIPKKG----NYNGVHIPNARAAKS-----PLLNG 125
           S +SV+ V+Q NFHEL+ +RVEVKRAIPK+G    N N V+IP + ++       P  NG
Sbjct: 170 SEDSVEVVMQSNFHELSDKRVEVKRAIPKEGIQSNNGNAVNIPPSYSSFQATPYVPEQNG 229

Query: 126 NRNV-PFPYPYYG 129
              V  FP P +G
Sbjct: 230 YGMVLQFPPPVFG 242

[Close]

BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot
Match: Q8W034 (Heterogeneous nuclear ribonucleoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=RNP1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.6e-27
Identity = 77/180 (42.78%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           ++T+R    +   + +KIFVGGL   LT+EEFR YFE +G +TDV +M+D  TNRPRGFG
Sbjct: 95  LNTSRSSGGDAYNKTKKIFVGGLPPTLTDEEFRQYFEVYGPVTDVAIMYDQATNRPRGFG 154

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120
           FV+F+S ++VDSVL + FH+L+G++VEVKRA+PK  N  G         +S    G+   
Sbjct: 155 FVSFDSEDAVDSVLHKTFHDLSGKQVEVKRALPKDANPGG-------GGRSMGGGGSGG- 214

Query: 121 PFPYPYYGSGYPIYPG------FTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYG 175
              Y  YG     Y G      F      G  + SYGS+ YG     G GN  S+G  YG
Sbjct: 215 ---YQGYGGNESSYDGRMDSNRFLQHQSVGNGLPSYGSSGYG----AGYGN-GSNGAGYG 258

[Close]

BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot
Match: Q9VVE5 (RNA-binding protein Musashi homolog Rbp6 OS=Drosophila melanogaster OX=7227 GN=Rbp6 PE=2 SV=3)

HSP 1 Score: 98.6 bits (244), Expect = 1.0e-19
Identity = 62/139 (44.60%), Postives = 80/139 (57.55%), Query Frame = 0


Query: 14  RGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESVDSV 73
           R +KIFVGGLSA  T E+ +SYFE FG I D ++M D  TNR RGFGFVTF+S + VD V
Sbjct: 116 RTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKV 175

Query: 74  LQRNFHELNGRRVEVKRAIPKKGNYNGVHIP--------NARAAKSPLLNGNRNVP--FP 133
            + +FHE+N + VE K+A PK+     V +P          R+A   ++ G   +P  FP
Sbjct: 176 CEIHFHEINNKMVECKKAQPKE-----VMLPANLAKTRAAGRSAYDNIMWGLGTLPEGFP 235

Query: 134 YPYY-----GSGYPIYPGF 138
              Y     G GY  YP F
Sbjct: 236 AAAYAAYAAGRGYSGYPSF 249

[Close]

BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot
Match: Q96DH6 (RNA-binding protein Musashi homolog 2 OS=Homo sapiens OX=9606 GN=MSI2 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-18
Identity = 59/147 (40.14%), Postives = 81/147 (55.10%), Query Frame = 0


Query: 14  RGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESVDSV 73
           R +KIFVGGLSA    E+ + YFE FG++ D ++M D  TNR RGFGFVTFE+ + V+ V
Sbjct: 108 RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKV 167

Query: 74  LQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYY--GSGY 133
            + +FHE+N + VE K+A PK+     V  P     ++      R +P+    +  G G 
Sbjct: 168 CEIHFHEINNKMVECKKAQPKE-----VMFPPGTRGRA------RGLPYTMDAFMLGMGM 227

Query: 134 PIYPGF--THFPHYGAAVDSYGSNFYG 157
             YP F  T+   Y     SYG  F G
Sbjct: 228 LGYPNFVATYGRGYPGFAPSYGYQFPG 243

[Close]

BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot
Match: Q920Q6 (RNA-binding protein Musashi homolog 2 OS=Mus musculus OX=10090 GN=Msi2 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-18
Identity = 59/147 (40.14%), Postives = 81/147 (55.10%), Query Frame = 0


Query: 14  RGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESVDSV 73
           R +KIFVGGLSA    E+ + YFE FG++ D ++M D  TNR RGFGFVTFE+ + V+ V
Sbjct: 108 RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKV 167

Query: 74  LQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYY--GSGY 133
            + +FHE+N + VE K+A PK+     V  P     ++      R +P+    +  G G 
Sbjct: 168 CEIHFHEINNKMVECKKAQPKE-----VMFPPGTRGRA------RGLPYTMDAFMLGMGM 227

Query: 134 PIYPGF--THFPHYGAAVDSYGSNFYG 157
             YP F  T+   Y     SYG  F G
Sbjct: 228 LGYPNFVATYGRGYPGFAPSYGYQFPG 243

[Close]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL
Match: A0A0A0L563 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G644730 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 4.4e-135
Identity = 234/238 (98.32%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG
Sbjct: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120
           FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV
Sbjct: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120

Query: 121 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSV 180
           PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGS NIASDGWW+GPNF SV
Sbjct: 121 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSANIASDGWWHGPNFFSV 180

Query: 181 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRSS 239
           GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDR+S
Sbjct: 181 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRTS 238

[Close]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL
Match: A0A6J1J2K5 (RNA-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481242 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 9.7e-66
Identity = 142/242 (58.68%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 4   TRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVT 63
           TRM+   DQ RGRKIFVGGLSAELTE++F++YFE FG++TDVVVM DSVT RPRGFGF+T
Sbjct: 5   TRME---DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFIT 64

Query: 64  FESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFP 123
           F+S++SVDSVLQ NFHELNGRRVEVKRAIPKKGNYNG HI     +KSPL     NV   
Sbjct: 65  FDSVQSVDSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-----SKSPL--SESNVLPS 124

Query: 124 YPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSVGML 183
           YPYY  GY +YPGFT FP YG     YGS+ YGNWF P  G +AS   WYGPNF   GM 
Sbjct: 125 YPYYSCGYTVYPGFTPFPPYGPGFGLYGSDPYGNWFSPSFGPMASPAAWYGPNFF--GMF 184

Query: 184 PPLCRNSLMHPSKGIYIGSRNGMANTRNG--------EIKQNTAGKRIMSADAAPFQIKD 238
           PP  +NSL++P+      S NG++    G        +++  T  ++ MSADA PF  KD
Sbjct: 185 PPPHKNSLVYPTY-----SSNGLSTPVAGYETADARRKVEPPTRAQKNMSADAVPFHAKD 229

[Close]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL
Match: A0A6J1FS87 (RNA-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446397 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 4.1e-64
Identity = 137/235 (58.30%), Postives = 163/235 (69.36%), Query Frame = 0


Query: 11  DQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESV 70
           DQ RGRKIFVGGLSAELTE++F++YFE FG++TDVVVM DSVT RPRGFGF+TF+S++SV
Sbjct: 9   DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFITFDSVQSV 68

Query: 71  DSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYYGSG 130
           DSVLQ NFHELNGRRVEVKRAIPKKGNYNG HI     +KSPL     NV   YPYY  G
Sbjct: 69  DSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-----SKSPL--SESNVLPSYPYYSCG 128

Query: 131 YPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSVGMLPPLCRNS 190
           Y +YPGFT FP YG     YGS+ YGNWF P  G +AS   WY PNF   GM PP  +NS
Sbjct: 129 YTVYPGFTPFPPYGPGFGLYGSDPYGNWFSPSFGPMASPAAWYAPNFF--GMFPPPHKNS 188

Query: 191 LMHPSKGIYIGSRNGMANTRNG--------EIKQNTAGKRIMSADAAPFQIKDRS 238
           L++P+      S NG++    G        ++   T  ++ MSADA PF  KD++
Sbjct: 189 LVYPTY-----SSNGLSTPVAGYETADARRKVDPPTRAQKNMSADAVPFHAKDKA 229

[Close]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL
Match: A0A6J1C724 (RNA-binding protein 1-like OS=Momordica charantia OX=3673 GN=LOC111008498 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.9e-53
Identity = 125/243 (51.44%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 2   STTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGF 61
           S+ RM++NNDQ RG+KIFVGGL ++LTE EF+SYFE FGQITDVVVM DSVTNRPRGFGF
Sbjct: 4   SSNRMESNNDQSRGKKIFVGGLPSDLTENEFKSYFEKFGQITDVVVMQDSVTNRPRGFGF 63

Query: 62  VTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVP 121
           +TF+S+ESV+ VLQ NFHELNGRRVEVKRA+PK  N N     N +  KSPL   + +VP
Sbjct: 64  ITFDSVESVNYVLQNNFHELNGRRVEVKRAVPKNRNCNS---SNPQRGKSPL---SPHVP 123

Query: 122 FPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPP---GSGNIAS----DGWWYG 181
             YP++ SG  +YPG+  FPHY      YGS+ YG+W      G+G+ A        WY 
Sbjct: 124 LYYPHFSSGPVVYPGYAPFPHY------YGSSLYGSWHYNGLYGTGHEAPPLPLGSAWYA 183

Query: 182 PNFLSVGMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIK 238
           PN    GM P    NS++HP   +Y      + +   G  ++N      MSADA PFQ+ 
Sbjct: 184 PNVFGDGMFPSPYINSVIHP---VYSSKGGRLLDPMAGNGRRNGN----MSADAVPFQLN 227

[Close]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL
Match: A0A2I4DTF8 (heterogeneous nuclear ribonucleoprotein 1-like OS=Juglans regia OX=51240 GN=LOC108983296 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 9.2e-40
Identity = 103/210 (49.05%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 7   DNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFES 66
           DN ++  R +KIFVGGLSA +TEEEF++YFE FG++TDVVVMHDS T+RPRGFGF+TF+S
Sbjct: 105 DNGDNLFRTKKIFVGGLSASITEEEFKNYFERFGKVTDVVVMHDSTTHRPRGFGFITFDS 164

Query: 67  LESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPY 126
            E+V +V+Q +FHELNGR VEVKRA+PK+ N    +  N R      + G    P  YP 
Sbjct: 165 EEAVVNVMQNSFHELNGRLVEVKRAVPKEANNGSDNGFNMR------IEGRGGPPGNYPP 224

Query: 127 YGSGYPIYPGFTHFPHYGAAVDS-YGSNFYGNWFPP-GSGNIAS-------DGWWYGPNF 186
           Y  GY I PG+  FP + +   S YG+  YG W+P  GSG I S          WYGP  
Sbjct: 225 YSPGYWILPGYAPFPGFSSGGGSLYGAGIYGGWYPTGGSGGIGSAVAPFAPRSSWYGPRG 284

Query: 187 LSVGMLPPLCRNSLMHPSKGIYIGSRNGMA 208
            S+G + P     L H S  IY G  +G A
Sbjct: 285 -SIGRVFP-----LPHSSGSIYAGYLSGGA 302

[Close]

BLAST of CSPI04G25000 vs. NCBI nr
Match: XP_004141412.1 (RNA-binding protein 1 [Cucumis sativus])

HSP 1 Score: 490.3 bits (1261), Expect = 9.2e-135
Identity = 234/238 (98.32%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG
Sbjct: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120
           FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV
Sbjct: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120

Query: 121 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSV 180
           PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGS NIASDGWW+GPNF SV
Sbjct: 121 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSANIASDGWWHGPNFFSV 180

Query: 181 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRSS 239
           GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDR+S
Sbjct: 181 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRTS 238

[Close]

BLAST of CSPI04G25000 vs. NCBI nr
Match: KAE8649895.1 (hypothetical protein Csa_012205 [Cucumis sativus])

HSP 1 Score: 490.3 bits (1261), Expect = 9.2e-135
Identity = 234/238 (98.32%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG
Sbjct: 53  MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 112

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120
           FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV
Sbjct: 113 FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 172

Query: 121 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSV 180
           PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGS NIASDGWW+GPNF SV
Sbjct: 173 PFPYPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSANIASDGWWHGPNFFSV 232

Query: 181 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRSS 239
           GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDR+S
Sbjct: 233 GMLPPLCRNSLMHPSKGIYIGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQIKDRTS 290

[Close]

BLAST of CSPI04G25000 vs. NCBI nr
Match: XP_038898856.1 (RNA-binding protein 1-like [Benincasa hispida])

HSP 1 Score: 383.3 bits (983), Expect = 1.6e-102
Identity = 192/245 (78.37%), Postives = 209/245 (85.31%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           MSTTRM+NNNDQCRGRKIFVGGLSAELTE+EF++YFE FG+ITDVVVMHDSVTNRPRGFG
Sbjct: 3   MSTTRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFG 62

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNAR--AAKSPLLNGNR 120
           F+TFES+ESVDSVLQ NFHELNGRRVEVKRAIPKKGNYNG HIPNAR    KSPL N NR
Sbjct: 63  FITFESVESVDSVLQSNFHELNGRRVEVKRAIPKKGNYNGGHIPNARTQGGKSPLPNSNR 122

Query: 121 NVPFPYPYYGSGYPIYPGFTHFPHYGA-AVDSYGSNFYGNWFPPGSGNIASDGWWYGPNF 180
           NVPFPYPYYGS YP+YPGFT FPHYG   VD YGS+ YGNWFPP  GN+AS+G WYGPNF
Sbjct: 123 NVPFPYPYYGSSYPVYPGFTPFPHYGGPGVDLYGSHLYGNWFPPSFGNMASEGTWYGPNF 182

Query: 181 LSVGMLPPLCRNSLMHP---SKGIY-IGSRNGMANT-RNGEIKQNTAGKRIMSADAAPFQ 238
             VG+ PP  RNSL+ P   SKG+   G+ NGM +T RNGEIKQNT GKRIMSADA PFQ
Sbjct: 183 FGVGIFPPPYRNSLIPPIYSSKGLSDTGTGNGMVDTRRNGEIKQNTTGKRIMSADAVPFQ 242

[Close]

BLAST of CSPI04G25000 vs. NCBI nr
Match: XP_022982410.1 (RNA-binding protein 1-like [Cucurbita maxima])

HSP 1 Score: 260.0 bits (663), Expect = 2.0e-65
Identity = 142/242 (58.68%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 4   TRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVT 63
           TRM+   DQ RGRKIFVGGLSAELTE++F++YFE FG++TDVVVM DSVT RPRGFGF+T
Sbjct: 5   TRME---DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFIT 64

Query: 64  FESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFP 123
           F+S++SVDSVLQ NFHELNGRRVEVKRAIPKKGNYNG HI     +KSPL     NV   
Sbjct: 65  FDSVQSVDSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-----SKSPL--SESNVLPS 124

Query: 124 YPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSVGML 183
           YPYY  GY +YPGFT FP YG     YGS+ YGNWF P  G +AS   WYGPNF   GM 
Sbjct: 125 YPYYSCGYTVYPGFTPFPPYGPGFGLYGSDPYGNWFSPSFGPMASPAAWYGPNFF--GMF 184

Query: 184 PPLCRNSLMHPSKGIYIGSRNGMANTRNG--------EIKQNTAGKRIMSADAAPFQIKD 238
           PP  +NSL++P+      S NG++    G        +++  T  ++ MSADA PF  KD
Sbjct: 185 PPPHKNSLVYPTY-----SSNGLSTPVAGYETADARRKVEPPTRAQKNMSADAVPFHAKD 229

[Close]

BLAST of CSPI04G25000 vs. NCBI nr
Match: XP_023524364.1 (RNA-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-64
Identity = 140/242 (57.85%), Postives = 170/242 (70.25%), Query Frame = 0


Query: 4   TRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVT 63
           TRM+   DQ RGRKIFVGGLSAELTE++F++YFE FG++TDVVVM DSVT RPRGFGF+T
Sbjct: 5   TRME---DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFIT 64

Query: 64  FESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFP 123
           F+S++SVDSVLQ NFHELNGRRVEVKRAIPKKGNYNG HI     +KSPL     NV   
Sbjct: 65  FDSVQSVDSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-----SKSPL--SESNVLPS 124

Query: 124 YPYYGSGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYGPNFLSVGML 183
           YPYY  GY +YPGFT FP YG     YGS+ YGNWF P  G +AS   WY PNF   GM 
Sbjct: 125 YPYYSCGYTVYPGFTPFPPYGPGFGLYGSDPYGNWFSPSFGPMASPAAWYAPNFF--GMF 184

Query: 184 PPLCRNSLMHPSKGIYIGSRNGMA------NTRNG--EIKQNTAGKRIMSADAAPFQIKD 238
           PP  +NSL++P+      S NG++       T +G  +++  +  ++ MSADA PF  KD
Sbjct: 185 PPPHKNSLVYPTY-----SSNGLSTPVAGYETADGRRKVEPPSRAQKNMSADAVPFHAKD 229

[Close]

BLAST of CSPI04G25000 vs. TAIR 10
Match: AT1G58470.1 (RNA-binding protein 1 )

HSP 1 Score: 136.7 bits (343), Expect = 2.4e-32
Identity = 76/133 (57.14%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 6   MDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFE 65
           M +N    R +KIFVGGLS+  TEEEF+SYFE FG+ TDVVVMHD VTNRPRGFGFVT++
Sbjct: 110 MSSNGVTSRTKKIFVGGLSSNTTEEEFKSYFERFGRTTDVVVMHDGVTNRPRGFGFVTYD 169

Query: 66  SLESVDSVLQRNFHELNGRRVEVKRAIPKKG----NYNGVHIPNARAAKS-----PLLNG 125
           S +SV+ V+Q NFHEL+ +RVEVKRAIPK+G    N N V+IP + ++       P  NG
Sbjct: 170 SEDSVEVVMQSNFHELSDKRVEVKRAIPKEGIQSNNGNAVNIPPSYSSFQATPYVPEQNG 229

Query: 126 NRNV-PFPYPYYG 129
              V  FP P +G
Sbjct: 230 YGMVLQFPPPVFG 242

[Close]

BLAST of CSPI04G25000 vs. TAIR 10
Match: AT4G26650.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.2e-28
Identity = 70/157 (44.59%), Postives = 91/157 (57.96%), Query Frame = 0


Query: 9   NNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLE 68
           N    R +KIFVGGL + +TE EF++YF+ FG I DVVVM+D  T RPRGFGF+TF+S E
Sbjct: 115 NGGGARTKKIFVGGLPSSITEAEFKNYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSEE 174

Query: 69  SVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYYG 128
           SVD VL + FHELNG+ VEVKRA+PK+       + +    +SPL+            YG
Sbjct: 175 SVDMVLHKTFHELNGKMVEVKRAVPKE-------LSSTTPNRSPLIG-----------YG 234

Query: 129 SGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGN 166
           + Y + P            +   +N Y N FPPG  N
Sbjct: 235 NNYGVVP------------NRSSANSYFNSFPPGYNN 241

[Close]

BLAST of CSPI04G25000 vs. TAIR 10
Match: AT4G26650.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.2e-28
Identity = 70/157 (44.59%), Postives = 91/157 (57.96%), Query Frame = 0


Query: 9   NNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLE 68
           N    R +KIFVGGL + +TE EF++YF+ FG I DVVVM+D  T RPRGFGF+TF+S E
Sbjct: 112 NGGGARTKKIFVGGLPSSITEAEFKNYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSEE 171

Query: 69  SVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYYG 128
           SVD VL + FHELNG+ VEVKRA+PK+       + +    +SPL+            YG
Sbjct: 172 SVDMVLHKTFHELNGKMVEVKRAVPKE-------LSSTTPNRSPLIG-----------YG 231

Query: 129 SGYPIYPGFTHFPHYGAAVDSYGSNFYGNWFPPGSGN 166
           + Y + P            +   +N Y N FPPG  N
Sbjct: 232 NNYGVVP------------NRSSANSYFNSFPPGYNN 238

[Close]

BLAST of CSPI04G25000 vs. TAIR 10
Match: AT4G14300.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 122.5 bits (306), Expect = 4.7e-28
Identity = 77/180 (42.78%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60
           ++T+R    +   + +KIFVGGL   LT+EEFR YFE +G +TDV +M+D  TNRPRGFG
Sbjct: 95  LNTSRSSGGDAYNKTKKIFVGGLPPTLTDEEFRQYFEVYGPVTDVAIMYDQATNRPRGFG 154

Query: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNV 120
           FV+F+S ++VDSVL + FH+L+G++VEVKRA+PK  N  G         +S    G+   
Sbjct: 155 FVSFDSEDAVDSVLHKTFHDLSGKQVEVKRALPKDANPGG-------GGRSMGGGGSGG- 214

Query: 121 PFPYPYYGSGYPIYPG------FTHFPHYGAAVDSYGSNFYGNWFPPGSGNIASDGWWYG 175
              Y  YG     Y G      F      G  + SYGS+ YG     G GN  S+G  YG
Sbjct: 215 ---YQGYGGNESSYDGRMDSNRFLQHQSVGNGLPSYGSSGYG----AGYGN-GSNGAGYG 258

[Close]

BLAST of CSPI04G25000 vs. TAIR 10
Match: AT5G55550.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-27
Identity = 77/175 (44.00%), Postives = 101/175 (57.71%), Query Frame = 0


Query: 14  RGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFGFVTFESLESVDSV 73
           R +KIFVGGL + +TEEEF++YF+ FG I DVVVM+D  T RPRGFGF+TF+S ++VD V
Sbjct: 108 RTKKIFVGGLPSSITEEEFKNYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSDDAVDRV 167

Query: 74  LQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNARAAKSPLLNGNRNVPFPYPYYGSGYPI 133
           L + FHELNG+ VEVKRA+PK+       I      +SPL +G          YG G   
Sbjct: 168 LHKTFHELNGKLVEVKRAVPKE-------ISPVSNIRSPLASGVN--------YGGGSNR 227

Query: 134 YPGFTHFPHYGAAVDSYGS-NFYGNWFPP--GSGNIASDGWWYGPNF-LSVGMLP 185
            P  ++F ++      Y S    G  F P  GSG  A   +  G N  LS+ + P
Sbjct: 228 MPANSYFNNFAPGPGFYNSLGPVGRRFSPVIGSGRNAVSAFGLGLNHDLSLNLNP 267

The following BLAST results are available for this feature:

BLAST of CSPI04G25000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C652	3.4e-31	57.14	RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBP1 PE=2 SV=1	[more]
Q8W034	6.6e-27	42.78	Heterogeneous nuclear ribonucleoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=RNP...	[more]
Q9VVE5	1.0e-19	44.60	RNA-binding protein Musashi homolog Rbp6 OS=Drosophila melanogaster OX=7227 GN=R...	[more]
Q96DH6	1.5e-18	40.14	RNA-binding protein Musashi homolog 2 OS=Homo sapiens OX=9606 GN=MSI2 PE=1 SV=1	[more]
Q920Q6	1.5e-18	40.14	RNA-binding protein Musashi homolog 2 OS=Mus musculus OX=10090 GN=Msi2 PE=1 SV=1	[more]

BLAST of CSPI04G25000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L563	4.4e-135	98.32	RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G644730 PE=4 SV...	[more]
A0A6J1J2K5	9.7e-66	58.68	RNA-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481242 PE=4 SV=1	[more]
A0A6J1FS87	4.1e-64	58.30	RNA-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446397 PE=4 SV...	[more]
A0A6J1C724	1.9e-53	51.44	RNA-binding protein 1-like OS=Momordica charantia OX=3673 GN=LOC111008498 PE=4 S...	[more]
A0A2I4DTF8	9.2e-40	49.05	heterogeneous nuclear ribonucleoprotein 1-like OS=Juglans regia OX=51240 GN=LOC1...	[more]

BLAST of CSPI04G25000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141412.1	9.2e-135	98.32	RNA-binding protein 1 [Cucumis sativus]	[more]
KAE8649895.1	9.2e-135	98.32	hypothetical protein Csa_012205 [Cucumis sativus]	[more]
XP_038898856.1	1.6e-102	78.37	RNA-binding protein 1-like [Benincasa hispida]	[more]
XP_022982410.1	2.0e-65	58.68	RNA-binding protein 1-like [Cucurbita maxima]	[more]
XP_023524364.1	6.5e-64	57.85	RNA-binding protein 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI04G25000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G58470.1	2.4e-32	57.14	RNA-binding protein 1	[more]
AT4G26650.1	1.2e-28	44.59	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT4G26650.2	1.2e-28	44.59	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT4G14300.1	4.7e-28	42.78	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G55550.1	2.3e-27	44.00	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 17..89 e-value: 3.1E-25 score: 99.8
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 18..86 e-value: 1.6E-17 score: 63.1
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 16..93 score: 19.160774
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 13..100 e-value: 1.1E-27 score: 98.0
None	No IPR available	PANTHER	PTHR48032:SF12	NUCLEOTIDE-BINDING ALPHA-BETA PLAIT DOMAIN-CONTAINING PROTEIN-RELATED	coord: 6..228
None	No IPR available	PANTHER	PTHR48032	RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6	coord: 6..228
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 16..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G25000.1	CSPI04G25000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding