CSPI04G24940 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G24940
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMixed-linked glucan synthase
LocationChr4: 22511983 .. 22512764 (+)
RNA-Seq ExpressionCSPI04G24940
SyntenyCSPI04G24940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGTTTAGTGATTTAACTTCCACTGTCTTTTACAAAATTTATACTCACCTTGTCGGTAATTTTAACAGTATGAAAGGGCATGCTTCAAAAATTCAACCCTTAATTATTAAAACAGGATCTATAGAAGATGCTTCTTTAGAACTTATAAACTGCTATAGGTTTAGAGCTTTCATAACTTTGTCTCTGTGTTTTCTCTCTCTGTTTCTGACTCACGGCTAAGGAAAGAGCTTAAAATTTCCTTTTTCATGGCTGCCATTACTTCCACTTGCAGTAGCTTCTTCTCCATCAGATCAAATTCTATGGAGCCAAGAGTTAGAACTTCTTCACCAAGCCATGGCTCTCCATCATGTGGGAAGCTTGATGGGGTGGCAACTTGGCTCATCAATGGCTTTGTCACAGCTTTCTTTGGATCGTTGGAACGATGCTCTTGTATTCGTATTGCAACGGCTGAGGACGATGGCGATGAGGGGAACGACATCCCCTTGATTCCGAACGACGGAAATCTTCGACAGGAGGGCACTGCAGGTGGGCGGAGGAGGGCTGGGAAAGGCAAGAAGTAAATGCAATCTAATGAGTTGCTTGCTTCTCATGTTGTATAATCCAATTTCAAATCGAAAAAGCTCCTCTTTCTCTTTGGTAAGTGATATAAAGGAGCTTCTGAATCTGAAATATTTACTACTTTTTCTTCTTCTTTTAATTTAGTTTTGTGGTGATGAAATCAACCAAATTACTTATAATATTTGTTCAGTGCTTTTAGAATCTATGAATCAAGAACTAAAA

mRNA sequence

CTTTGTTTAGTGATTTAACTTCCACTGTCTTTTACAAAATTTATACTCACCTTGTCGGTAATTTTAACAGTATGAAAGGGCATGCTTCAAAAATTCAACCCTTAATTATTAAAACAGGATCTATAGAAGATGCTTCTTTAGAACTTATAAACTGCTATAGGTTTAGAGCTTTCATAACTTTGTCTCTGTGTTTTCTCTCTCTGTTTCTGACTCACGGCTAAGGAAAGAGCTTAAAATTTCCTTTTTCATGGCTGCCATTACTTCCACTTGCAGTAGCTTCTTCTCCATCAGATCAAATTCTATGGAGCCAAGAGTTAGAACTTCTTCACCAAGCCATGGCTCTCCATCATGTGGGAAGCTTGATGGGGTGGCAACTTGGCTCATCAATGGCTTTGTCACAGCTTTCTTTGGATCGTTGGAACGATGCTCTTGTATTCGTATTGCAACGGCTGAGGACGATGGCGATGAGGGGAACGACATCCCCTTGATTCCGAACGACGGAAATCTTCGACAGGAGGGCACTGCAGGTGGGCGGAGGAGGGCTGGGAAAGGCAAGAAGTAAATGCAATCTAATGAGTTGCTTGCTTCTCATGTTGTATAATCCAATTTCAAATCGAAAAAGCTCCTCTTTCTCTTTGGTAAGTGATATAAAGGAGCTTCTGAATCTGAAATATTTACTACTTTTTCTTCTTCTTTTAATTTAGTTTTGTGGTGATGAAATCAACCAAATTACTTATAATATTTGTTCAGTGCTTTTAGAATCTATGAATCAAGAACTAAAA

Coding sequence (CDS)

ATGGCTGCCATTACTTCCACTTGCAGTAGCTTCTTCTCCATCAGATCAAATTCTATGGAGCCAAGAGTTAGAACTTCTTCACCAAGCCATGGCTCTCCATCATGTGGGAAGCTTGATGGGGTGGCAACTTGGCTCATCAATGGCTTTGTCACAGCTTTCTTTGGATCGTTGGAACGATGCTCTTGTATTCGTATTGCAACGGCTGAGGACGATGGCGATGAGGGGAACGACATCCCCTTGATTCCGAACGACGGAAATCTTCGACAGGAGGGCACTGCAGGTGGGCGGAGGAGGGCTGGGAAAGGCAAGAAGTAA

Protein sequence

MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERCSCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK*
Homology
BLAST of CSPI04G24940 vs. ExPASy TrEMBL
Match: A0A0A0L2M3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G644660 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.9e-51
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK
Sbjct: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 104

BLAST of CSPI04G24940 vs. ExPASy TrEMBL
Match: A0A5D3D9D4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002500 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 4.3e-50
Identity = 102/104 (98.08%), Postives = 103/104 (99.04%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDEGNDIPLIPNDGNLRQ+GTA GRRRAGKGKK
Sbjct: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQDGTAAGRRRAGKGKK 104

BLAST of CSPI04G24940 vs. ExPASy TrEMBL
Match: A0A6J1IIU9 (uncharacterized protein LOC111477268 OS=Cucurbita maxima OX=3661 GN=LOC111477268 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.2e-44
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRS+SMEPRVRTSSPSH SP+CGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 91  MAAITSTCSSFFSIRSSSMEPRVRTSSPSHASPACGKLDGVATWLINGFVTAFFGSLERC 150

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDE ND PLIPNDGNL+Q+G A GRRR  KGKK
Sbjct: 151 SCIRIATAEDDGDEANDAPLIPNDGNLQQDGAAAGRRRTVKGKK 194

BLAST of CSPI04G24940 vs. ExPASy TrEMBL
Match: A0A2R6R383 (Protease OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc11731 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.0e-31
Identity = 73/105 (69.52%), Postives = 86/105 (81.90%), Query Frame = 0

Query: 1   MAAIT-STCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLER 60
           MAA T S+CSSFF++R+N +EPRVR SSPSHGSP CGK DGV  WLING  +AFF SL+R
Sbjct: 1   MAATTSSSCSSFFNLRANPVEPRVR-SSPSHGSPGCGKQDGVTMWLINGVASAFFASLDR 60

Query: 61  CSCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           CSCIRI+T EDDGD+ N++PLI NDGN R +  +GGRRR GKGKK
Sbjct: 61  CSCIRISTHEDDGDDANNLPLIFNDGNYRLDDRSGGRRRTGKGKK 104

BLAST of CSPI04G24940 vs. ExPASy TrEMBL
Match: A0A2P5CWX9 (Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_115610 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.4e-31
Identity = 77/105 (73.33%), Postives = 84/105 (80.00%), Query Frame = 0

Query: 1   MAAITST-CSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLER 60
           MAA TST C+SFFS+RSN MEP+VRTSS SHGSP CGK+DGVA WLIN   TAFF SLER
Sbjct: 1   MAATTSTSCTSFFSLRSNPMEPKVRTSS-SHGSPGCGKVDGVAMWLINSVTTAFFASLER 60

Query: 61  CSCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           CSCIRIAT  DDGD+ ND+PLI NDGNLR +G    RRR GKG K
Sbjct: 61  CSCIRIATV-DDGDDANDLPLIFNDGNLRHDGGTISRRRTGKGNK 103

BLAST of CSPI04G24940 vs. NCBI nr
Match: KAA0064464.1 (hypothetical protein E6C27_scaffold255G001780 [Cucumis melo var. makuwa] >TYK20124.1 hypothetical protein E5676_scaffold134G002500 [Cucumis melo var. makuwa])

HSP 1 Score: 206.8 bits (525), Expect = 8.9e-50
Identity = 102/104 (98.08%), Postives = 103/104 (99.04%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDEGNDIPLIPNDGNLRQ+GTA GRRRAGKGKK
Sbjct: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQDGTAAGRRRAGKGKK 104

BLAST of CSPI04G24940 vs. NCBI nr
Match: XP_038897734.1 (uncharacterized protein LOC120085674 isoform X1 [Benincasa hispida])

HSP 1 Score: 198.7 bits (504), Expect = 2.4e-47
Identity = 97/104 (93.27%), Postives = 101/104 (97.12%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRSNSMEPR+RTSS SHGSP+CGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 27  MAAITSTCSSFFSIRSNSMEPRLRTSSSSHGSPACGKLDGVATWLINGFVTAFFGSLERC 86

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDE ND+PLIPNDGNLRQ+GTA GRRRAGKGKK
Sbjct: 87  SCIRIATAEDDGDEANDVPLIPNDGNLRQDGTAAGRRRAGKGKK 130

BLAST of CSPI04G24940 vs. NCBI nr
Match: XP_038897735.1 (uncharacterized protein LOC120085674 isoform X2 [Benincasa hispida])

HSP 1 Score: 198.7 bits (504), Expect = 2.4e-47
Identity = 97/104 (93.27%), Postives = 101/104 (97.12%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRSNSMEPR+RTSS SHGSP+CGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 16  MAAITSTCSSFFSIRSNSMEPRLRTSSSSHGSPACGKLDGVATWLINGFVTAFFGSLERC 75

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDE ND+PLIPNDGNLRQ+GTA GRRRAGKGKK
Sbjct: 76  SCIRIATAEDDGDEANDVPLIPNDGNLRQDGTAAGRRRAGKGKK 119

BLAST of CSPI04G24940 vs. NCBI nr
Match: KAG6591437.1 (hypothetical protein SDJN03_13783, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 189.5 bits (480), Expect = 1.5e-44
Identity = 93/104 (89.42%), Postives = 98/104 (94.23%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRS+SMEPRVRTSSP+H SP+CGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 63  MAAITSTCSSFFSIRSSSMEPRVRTSSPTHASPACGKLDGVATWLINGFVTAFFGSLERC 122

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDE ND PLIPNDGNL+Q+GTA GRRR  KGKK
Sbjct: 123 SCIRIATAEDDGDEANDAPLIPNDGNLQQDGTAAGRRRTVKGKK 166

BLAST of CSPI04G24940 vs. NCBI nr
Match: XP_022977101.1 (uncharacterized protein LOC111477268 [Cucurbita maxima])

HSP 1 Score: 188.7 bits (478), Expect = 2.5e-44
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0

Query: 1   MAAITSTCSSFFSIRSNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERC 60
           MAAITSTCSSFFSIRS+SMEPRVRTSSPSH SP+CGKLDGVATWLINGFVTAFFGSLERC
Sbjct: 91  MAAITSTCSSFFSIRSSSMEPRVRTSSPSHASPACGKLDGVATWLINGFVTAFFGSLERC 150

Query: 61  SCIRIATAEDDGDEGNDIPLIPNDGNLRQEGTAGGRRRAGKGKK 105
           SCIRIATAEDDGDE ND PLIPNDGNL+Q+G A GRRR  KGKK
Sbjct: 151 SCIRIATAEDDGDEANDAPLIPNDGNLQQDGAAAGRRRTVKGKK 194

BLAST of CSPI04G24940 vs. TAIR 10
Match: AT4G10810.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G24026.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 52.4 bits (124), Expect = 2.6e-07
Identity = 27/67 (40.30%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 16 SNSMEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERCSCIRIATAEDD--GD 75
          S+S+     T+S +  + S  KLD  A+W+    ++AFF SLERCSC+ ++T++DD  G+
Sbjct: 4  SSSINSTASTAS-NLSTASLEKLDQAASWVSTTVISAFFASLERCSCVNLSTSDDDDEGE 63

Query: 76 EGNDIPL 81
          E ++ PL
Sbjct: 64 ESHNRPL 69

BLAST of CSPI04G24940 vs. TAIR 10
Match: AT4G24026.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G10810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-06
Identity = 21/59 (35.59%), Postives = 37/59 (62.71%), Query Frame = 0

Query: 19 MEPRVRTSSPSHGSPSCGKLDGVATWLINGFVTAFFGSLERCSCIRIATAEDDGDEGND 78
          M+  +  +S    + S  K+D  A+W+    ++AFF SLERC+C+ ++T+ DD D+ +D
Sbjct: 1  MDKSISITSNVSTTTSMEKIDHAASWISATVISAFFTSLERCACVNLSTSHDDDDDDDD 59

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L2M33.9e-51100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G644660 PE=4 SV=1[more]
A0A5D3D9D44.3e-5098.08Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1IIU91.2e-4489.42uncharacterized protein LOC111477268 OS=Cucurbita maxima OX=3661 GN=LOC111477268... [more]
A0A2R6R3832.0e-3169.52Protease OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc11731 PE=4... [more]
A0A2P5CWX93.4e-3173.33Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_115610 PE... [more]
Match NameE-valueIdentityDescription
KAA0064464.18.9e-5098.08hypothetical protein E6C27_scaffold255G001780 [Cucumis melo var. makuwa] >TYK201... [more]
XP_038897734.12.4e-4793.27uncharacterized protein LOC120085674 isoform X1 [Benincasa hispida][more]
XP_038897735.12.4e-4793.27uncharacterized protein LOC120085674 isoform X2 [Benincasa hispida][more]
KAG6591437.11.5e-4489.42hypothetical protein SDJN03_13783, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022977101.12.5e-4489.42uncharacterized protein LOC111477268 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G10810.12.6e-0740.30unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT4G24026.11.3e-0635.59unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..104
NoneNo IPR availablePANTHERPTHR34061:SF2PROTEIN, PUTATIVE-RELATEDcoord: 4..104
NoneNo IPR availablePANTHERPTHR34061PROTEIN, PUTATIVE-RELATEDcoord: 4..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G24940.1CSPI04G24940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008233 peptidase activity