Homology
BLAST of CSPI04G24600 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 475.3 bits (1222), Expect = 8.6e-133
Identity = 243/482 (50.41%), Postives = 322/482 (66.80%), Query Frame = 0
Query: 29 RNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGPVVYFRFGQRRVLVVSSPFAAEEC 88
RNLPP+P LPVIGHLHL+ ++R+L ++S YG V + G R VLVVSSP AAEEC
Sbjct: 30 RNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEEC 89
Query: 89 LTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWRNLRRISTIEILSTHRIQMLSMFR 148
TKNDIVFANRP I+ K+ GYN T +V S YG+HWRNLRR++ +EI S + R
Sbjct: 90 FTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIR 149
Query: 149 LEEVKSLIRRLANNEKQIH---NMKNEFFDLTYNVMLRMLVGKRFYGEDVDDVDEAKIFR 208
+EVK L+ L + Q MK++ +L++NV +RM+ GKR++G+DVD DEAK+FR
Sbjct: 150 EDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVDS-DEAKLFR 209
Query: 209 QLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHVKRDTFMQNLIDQHKKRIVDQ 268
L ++ + G S DF+PF+ W+ F E K+ + + D F+Q LI H+ RI
Sbjct: 210 ALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEKKVSKISQEMDAFLQRLI--HESRI--- 269
Query: 269 NKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALMLVLLAAGTETSVAAMEWALSL 328
NK+N TMI+ LL LQ+ P+ YTD+ I+ +++VLL AGT+TS +EWA+SL
Sbjct: 270 NKNN--------VTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSL 329
Query: 329 MLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGIINETLRMYPPAPLVVPHESSK 388
+LNHP+ L+K + EI+ QVG +RLI+E D+ L L II+ET R+ P AP++VPHESS
Sbjct: 330 LLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSD 389
Query: 389 DCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRFESLENEKFGFNLIPFGSGRR 448
DC + GY +P+GTIL VN WAIH DP+ WD P F P R +E E L+PFG GRR
Sbjct: 390 DCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVELEP--SKLMPFGMGRR 449
Query: 449 GCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVALTMPKAHCLQAKCTPRPIVH 508
CPG GLA RV+GL LGAL+QCFEW+R GE +DM EG LTMPKA L+A C PR I+H
Sbjct: 450 SCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILH 494
BLAST of CSPI04G24600 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 453.8 bits (1166), Expect = 2.7e-126
Identity = 245/514 (47.67%), Postives = 327/514 (63.62%), Query Frame = 0
Query: 3 VLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS-- 62
VL SIF L F++ + ++L K +NLPP+P LP+IGHL LLK PI+RTL S
Sbjct: 9 VLYSIF---SLIFLIIS-FKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSET 68
Query: 63 ---NHYGPVVYFRFGQRRVLVVSS-PFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLV 122
N G V+ R G R V VVSS AAEEC KND+V ANRP +II K GYNNTN++
Sbjct: 69 LDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMI 128
Query: 123 WSSYGDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLI---RRLANNEKQIHNMKNEF 182
+ YGDHWRNLRR+ TIEI STHR+ R +EV+ LI RLA +K + +K
Sbjct: 129 AAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPML 188
Query: 183 FDLTYNVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFG 242
DLT+N ++RM+ GKR+YGE+ D +EAK R+L D+G D++P +
Sbjct: 189 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--- 248
Query: 243 STIENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPD 302
S+ EN++ + + D F+Q LID + Q TMI+ LL LQ+ + +
Sbjct: 249 SSYENRVKKLGEETDKFLQGLIDDKR------------GQQETGTTMIDHLLVLQKSDIE 308
Query: 303 QYTDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDES 362
YTD+ I+ ++L+++ AGT TS +EWALS +LNHP + K ++EIDN+VG DRLI+E+
Sbjct: 309 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEA 368
Query: 363 DMANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKI 422
D++ LP L+ I+ ETLR++P PL+VPH +S+DC IG Y +PRGT L VN WAIH DP
Sbjct: 369 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 428
Query: 423 WDNPRKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERP 482
WD+P F P RFE E + L+ FG GRR CPG GLA R++GL LG+L+QCFEWER
Sbjct: 429 WDDPDSFKPERFEKEEEAQ---KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERV 488
Query: 483 GEELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
G VDM EGV T+PKA L+A C RP +H++
Sbjct: 489 GNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKI 500
BLAST of CSPI04G24600 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 437.6 bits (1124), Expect = 2.0e-121
Identity = 230/498 (46.18%), Postives = 316/498 (63.45%), Query Frame = 0
Query: 11 IPLFFVLYTLIEYL--IHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGPVVY 70
I LFF++ TL + K +NLPP P LP+IG+LH LK P++ T +S YG +
Sbjct: 10 ISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 69
Query: 71 FRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWRNLR 130
FG R V+VVSS A+EC TKNDIV ANRP + K+ GYNNT + S YGDHWRNLR
Sbjct: 70 LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 129
Query: 131 RISTIEILSTHRIQMLSMFRLEEVKSLIRRLAN---NEKQIHNMKNEFFDLTYNVMLRML 190
RI +IEILS+HR+ R +E+ LI++LA N ++ F ++T+N ++RM+
Sbjct: 130 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 189
Query: 191 VGKRFYGEDVD--DVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIEC 250
GKR+YG D D DV+EA++FR + ++ LGG + + DF+ F+ W F +E ++ +
Sbjct: 190 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG-LEKRLKKI 249
Query: 251 HVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRAL 310
+ D F+Q LID+H+ + SN TMI+ LL QQ P+ YTD+ I+ L
Sbjct: 250 SKRTDAFLQGLIDEHR---FGKRNSN---------TMIDHLLTQQQSQPEYYTDQIIKGL 309
Query: 311 MLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRG 370
M+V+L AGT+TS +EWA+S +LNHP+ +KK +NE+D +GHDR +DE D++ LP L+
Sbjct: 310 MVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQS 369
Query: 371 IINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPN 430
I+ ETLR++ APL+VPH SS+D ++GGY+IP+ TIL VN W IH DP +W +P F P
Sbjct: 370 IVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPE 429
Query: 431 RFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEG 490
RF E E L+ FG GRR CPGE L+ R GL LG L+QCFEW+R GEE +DM E
Sbjct: 430 RF---EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEA 489
Query: 491 VALTMPKAHCLQAKCTPR 502
+T K L A C R
Sbjct: 490 KGITAGKKTSLNAMCKVR 490
BLAST of CSPI04G24600 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 433.3 bits (1113), Expect = 3.8e-120
Identity = 232/515 (45.05%), Postives = 324/515 (62.91%), Query Frame = 0
Query: 3 VLESIFVYIPLFFVLYTLIEYLIHKIR----NLPPTPFPLLPVIGHLHLLKNPIYRTLAE 62
++E+I++ + LFF L L+ R NLPP+P P+IGHLHLLK P++R
Sbjct: 7 IMETIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLS 66
Query: 63 ISNHYGPVVYF--RFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLV 122
+S F G R V VVSS AEEC TKND+V ANRP ++ K GYN+T +V
Sbjct: 67 LSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMV 126
Query: 123 WSSYGDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIH---NMKNEF 182
++YGD WRNLRRI TIEI S+ R+ R +E++ LI LA N + MK F
Sbjct: 127 GAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLF 186
Query: 183 FDLTYNVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFG 242
LT N ++RM+ GKRFYG+ ++ +EAK RQL ++ GG D+ P + ++
Sbjct: 187 MCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--- 246
Query: 243 STIENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPD 302
+ E + + + D F+Q+L+++ + V++ K N TMI+ LL LQ+ PD
Sbjct: 247 TNYEKHVKKLAGRVDEFLQSLVNEKR---VEKVKGN---------TMIDHLLSLQETQPD 306
Query: 303 QYTDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDES 362
YTD I+ ++LV++ AGT+TS +EWA+S +LNHP+ L+K + EID+Q+G DRL++E
Sbjct: 307 YYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQ 366
Query: 363 DMANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKI 422
D+ LP L+ I++ETLR+YP AP+++PH +S+DC + GY +PRGTI+ VN WAIH DPK+
Sbjct: 367 DIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKL 426
Query: 423 WDNPRKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERP 482
W+ P KF P RFE +K L+PFG GRR CPG GLA R++ L LG+LVQCFEWER
Sbjct: 427 WEEPEKFKPERFEKKGEDK---KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERV 486
Query: 483 GEELVDMTEG-VALTMPKAHCLQAKCTPRPIVHRL 508
E+ +DM E TM KA LQA C RPIVH++
Sbjct: 487 EEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKV 503
BLAST of CSPI04G24600 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 430.3 bits (1105), Expect = 3.2e-119
Identity = 220/513 (42.88%), Postives = 321/513 (62.57%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
+ +L Y+ LFF+ +I K +NLPP P P LP+IG+LH LK P++RT +S
Sbjct: 4 LSLLSYSVFYLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLS 63
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
YG V FG R V+VVSS ++C TKND+V ANRP + K+ YN T L +SY
Sbjct: 64 EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 123
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIH---NMKNEFFDLT 180
G+HWRNLRRI+ +++LS HRI S R +E + LI RLA++ + + +D+T
Sbjct: 124 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 183
Query: 181 YNVMLRMLVGKRFYGEDVD--DVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGST 240
+N ++RM+ GKR+YGED D D+ EA FR + +L QL G + DF+P + ++ F
Sbjct: 184 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-EN 243
Query: 241 IENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQY 300
+E ++ + K D F++ LI++H+ ++ R TMI+ LL LQ P+ Y
Sbjct: 244 LEKRLKDISGKTDAFLRGLIEEHR------------TKKERANTMIDHLLNLQDSQPEYY 303
Query: 301 TDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDM 360
TD+ I+ L L +L AGT++S +EW++S +LNHP+ LKK+++E+D VG DRL+DESD+
Sbjct: 304 TDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDL 363
Query: 361 ANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWD 420
L L+ +INETLR+Y PAPL++PH +S +C IGGY +P+ TI+ +N WAIH DP++W
Sbjct: 364 PKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWT 423
Query: 421 NPRKFNPNRFESL-ENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPG 480
F P RFE E EK LI FG GRR CPGEGLA+R I + L L+QCF+W+
Sbjct: 424 EATTFKPERFEKKGELEK----LIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLIN 483
Query: 481 EELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
+ +D+ E T+ K L+A C RP+++++
Sbjct: 484 GDKIDLAERDGFTLTKLVPLKAMCKSRPVINKV 496
BLAST of CSPI04G24600 vs. ExPASy TrEMBL
Match:
A0A0A0L2I7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1)
HSP 1 Score: 1048.9 bits (2711), Expect = 7.0e-303
Identity = 514/516 (99.61%), Postives = 514/516 (99.61%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS
Sbjct: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRP LIISKWFGYNNTNLVWSSY
Sbjct: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNV 180
GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNE QIHNMKNEFFDLTYNV
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNENQIHNMKNEFFDLTYNV 180
Query: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI
Sbjct: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
Query: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI
Sbjct: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
Query: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS
Sbjct: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
Query: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF
Sbjct: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
Query: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM
Sbjct: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
Query: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 517
TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP
Sbjct: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516
BLAST of CSPI04G24600 vs. ExPASy TrEMBL
Match:
A0A1S3BUR6 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1)
HSP 1 Score: 969.5 bits (2505), Expect = 5.4e-279
Identity = 467/516 (90.50%), Postives = 490/516 (94.96%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
MGVL SIFVY PLF VLY LIE+L+HKIRNLPPTPFPLLP+IGHLHLLK PIYRTLA+IS
Sbjct: 1 MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
NHYGPVVYFRFG R+VLVVSS FAAEECLTKND+VFANRP LIISKWFGYNNTNL+WSSY
Sbjct: 61 NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNV 180
GDHWRNLRRISTIEILSTHR+QM S RLEEVKSLIRRLAN+E QIHNMKNEFFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180
Query: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
MLRMLVGKR+YGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIP VSWMGFGST+ENKI
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240
Query: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
IE HVKRDTFMQNLIDQHKKR DQN SN I QDGRRKTMIEVLLELQQY P+QYTDETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300
Query: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
RALML+LLAAGTETSV MEWALSLMLNHP+FLKKLQNEID+QVGHDRLIDESDMANLPS
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360
Query: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
LRGIINET RMYPPAPL+VPHESSKDC +GGYHIPRGTIL+VNLWAIHNDPKIWDNPRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420
Query: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
NPNRFESLE+EKFGFNL+PFGSGRRGCPGEGL LRVIGLVLG+LVQCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480
Query: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 517
TEG+ALTMPKAHCLQAKCTPRPIVHRLHLSHK SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516
BLAST of CSPI04G24600 vs. ExPASy TrEMBL
Match:
A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)
HSP 1 Score: 796.2 bits (2055), Expect = 8.2e-227
Identity = 383/510 (75.10%), Postives = 440/510 (86.27%), Query Frame = 0
Query: 6 SIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGP 65
+I VYIPLFFVLY L E +H+IRNLPPTPF LP+IGHLHLLK P+YRTLAEISN YGP
Sbjct: 3 AIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRYGP 62
Query: 66 VVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWR 125
VV+ RFG R VLVVSSP AAEECL+KND+VFA+RP L+ISKWFGYNN NLVWSSYGDHWR
Sbjct: 63 VVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDHWR 122
Query: 126 NLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNVMLRML 185
NLRRIST+EILSTHR+QML+ RL+EVKSLI RLA E Q+ +M N FFDLT+NVMLRM+
Sbjct: 123 NLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLRMI 182
Query: 186 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 245
VGKRFYGEDV+D DEA FR+LQ QLGGK+ + DFIP +SW+G G E +I+ECH
Sbjct: 183 VGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMECHQ 242
Query: 246 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 305
KRD FMQNLI+QHK R ++ N+ +DGR+KT+IEVLLELQ+ +PDQY DETIRALML
Sbjct: 243 KRDAFMQNLINQHKNRAIETEPDNSF-RDGRKKTLIEVLLELQESDPDQYNDETIRALML 302
Query: 306 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 365
VLLAAGTETS+ MEWALSLMLN+P+ L+K Q EID +VGHDRL+DESDMAN+P LRGII
Sbjct: 303 VLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGII 362
Query: 366 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 425
NETLRMYPPAPL+VPHESSKDC++GGYHIPRGT+LFVNLWAIHNDPKIW +PRKF+P+RF
Sbjct: 363 NETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDRF 422
Query: 426 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVA 485
+E+EK GF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCFEWERPGEELVDMTEGVA
Sbjct: 423 VKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGVA 482
Query: 486 LTMPKAHCLQAKCTPRPIVHRLHLSHKESN 516
++MPKA LQAKC PRPIV RLHLS +SN
Sbjct: 483 VSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511
BLAST of CSPI04G24600 vs. ExPASy TrEMBL
Match:
A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)
HSP 1 Score: 793.9 bits (2049), Expect = 4.1e-226
Identity = 381/510 (74.71%), Postives = 441/510 (86.47%), Query Frame = 0
Query: 6 SIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGP 65
+I +YIPLF VLY L E IHKIRNLPPTPF LP+IGHLHLLK P+YRTLAEISN YGP
Sbjct: 3 AIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRYGP 62
Query: 66 VVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWR 125
VV+ RFG R VLVVSSP AAEECL+KND+VFA+RP L+ISKWFGYNN NLVWSSYGDHWR
Sbjct: 63 VVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDHWR 122
Query: 126 NLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNVMLRML 185
NLRRIST+EILSTHR+QML+ RL+EVKSLI RLA E Q+ +M N FFDLT+NVMLRM+
Sbjct: 123 NLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLRMI 182
Query: 186 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 245
VGKRFYGEDV+D DEA FR+LQ QLGGK+ + DFIP +SW+G G E +I+ECH
Sbjct: 183 VGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMECHQ 242
Query: 246 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 305
KRD FMQNLI+QHK R + + + +N+ +DGR+KT+IEVLLELQ+ +PDQY DETIRALML
Sbjct: 243 KRDAFMQNLINQHKNRAI-ETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALML 302
Query: 306 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 365
VLLAAGTETS+ MEWALSLMLN+P+ L+K Q EID +VGHDRL+DESDMAN+P LRGII
Sbjct: 303 VLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGII 362
Query: 366 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 425
NETLRMYPPAPL+VPHESSKDC++GGYHIPRGT+LFVNLWAIHNDPKIW +PRKF+P+RF
Sbjct: 363 NETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDRF 422
Query: 426 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVA 485
+++EK GF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCFEWERPGE+LVDMTEGVA
Sbjct: 423 VKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGVA 482
Query: 486 LTMPKAHCLQAKCTPRPIVHRLHLSHKESN 516
++MPKA LQAKC PRPIV RLHLS +SN
Sbjct: 483 VSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511
BLAST of CSPI04G24600 vs. ExPASy TrEMBL
Match:
A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)
HSP 1 Score: 747.3 bits (1928), Expect = 4.4e-212
Identity = 360/514 (70.04%), Postives = 437/514 (85.02%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
+ + +IF+Y PLF VLY L E+ +HKIRNLPPTPFP LP+IGHL+LL PIYRTLA+IS
Sbjct: 6 LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
N +GPVV+ RFG R VLVVSSP AEECLTKND+VFANRP L+ISK FGYNNTNLVWSSY
Sbjct: 66 NRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSY 125
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQ--IHNMKNEFFDLTY 180
GDHWR+LRRIST+E+LSTHR+Q+LS RLEEV+SLIR+LA +E Q I M++ FFDLT+
Sbjct: 126 GDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTF 185
Query: 181 NVMLRMLVGKRFYGEDVDDVDE-AKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIE 240
NVMLRM+ GKRFYG+DVDD DE AKIFR+LQ + +L G S + DF+P ++W+GFG ++E
Sbjct: 186 NVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVE 245
Query: 241 NKIIECHVKRDTFMQNLIDQHKKRIVDQNKS--NNISQDGRRKTMIEVLLELQQYNPDQY 300
K+++CH KRD FMQ LI++ +KR + +S ++ +DGRRKTMIEVLLELQ+ PD Y
Sbjct: 246 KKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHY 305
Query: 301 TDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDM 360
TDE+I+ALMLVLL AGTETSV MEWALSL+LNHP+ LKK Q EID+QVG DRLIDESDM
Sbjct: 306 TDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDM 365
Query: 361 ANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWD 420
A+LP LRGII ET RMYPPAPL+VPH+SS+DC++GG+HIPRGT+L+VNLWAIHNDPKIW
Sbjct: 366 AHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWA 425
Query: 421 NPRKFNPNRFESLENEK--FGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERP 480
NP KF+P+RF+ LE++K +GFNL+PFGSGRRGCPGEGL LR+IGLVLG+L+QCFEWERP
Sbjct: 426 NPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERP 485
Query: 481 GEELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
GE+LVDMTEG+A++MPKA LQAKC PRPI+ L
Sbjct: 486 GEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANL 519
BLAST of CSPI04G24600 vs. NCBI nr
Match:
XP_004141397.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55249.1 hypothetical protein Csa_012321 [Cucumis sativus])
HSP 1 Score: 1048.9 bits (2711), Expect = 1.4e-302
Identity = 514/516 (99.61%), Postives = 514/516 (99.61%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS
Sbjct: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRP LIISKWFGYNNTNLVWSSY
Sbjct: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNV 180
GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNE QIHNMKNEFFDLTYNV
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNENQIHNMKNEFFDLTYNV 180
Query: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI
Sbjct: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
Query: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI
Sbjct: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
Query: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS
Sbjct: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
Query: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF
Sbjct: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
Query: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM
Sbjct: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
Query: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 517
TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP
Sbjct: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516
BLAST of CSPI04G24600 vs. NCBI nr
Match:
XP_008453068.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])
HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 467/516 (90.50%), Postives = 490/516 (94.96%), Query Frame = 0
Query: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
MGVL SIFVY PLF VLY LIE+L+HKIRNLPPTPFPLLP+IGHLHLLK PIYRTLA+IS
Sbjct: 1 MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60
Query: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSY 120
NHYGPVVYFRFG R+VLVVSS FAAEECLTKND+VFANRP LIISKWFGYNNTNL+WSSY
Sbjct: 61 NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120
Query: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNV 180
GDHWRNLRRISTIEILSTHR+QM S RLEEVKSLIRRLAN+E QIHNMKNEFFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180
Query: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
MLRMLVGKR+YGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIP VSWMGFGST+ENKI
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240
Query: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
IE HVKRDTFMQNLIDQHKKR DQN SN I QDGRRKTMIEVLLELQQY P+QYTDETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300
Query: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
RALML+LLAAGTETSV MEWALSLMLNHP+FLKKLQNEID+QVGHDRLIDESDMANLPS
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360
Query: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
LRGIINET RMYPPAPL+VPHESSKDC +GGYHIPRGTIL+VNLWAIHNDPKIWDNPRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420
Query: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
NPNRFESLE+EKFGFNL+PFGSGRRGCPGEGL LRVIGLVLG+LVQCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480
Query: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 517
TEG+ALTMPKAHCLQAKCTPRPIVHRLHLSHK SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516
BLAST of CSPI04G24600 vs. NCBI nr
Match:
XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 383/510 (75.10%), Postives = 440/510 (86.27%), Query Frame = 0
Query: 6 SIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGP 65
+I VYIPLFFVLY L E +H+IRNLPPTPF LP+IGHLHLLK P+YRTLAEISN YGP
Sbjct: 3 AIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRYGP 62
Query: 66 VVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWR 125
VV+ RFG R VLVVSSP AAEECL+KND+VFA+RP L+ISKWFGYNN NLVWSSYGDHWR
Sbjct: 63 VVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDHWR 122
Query: 126 NLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNVMLRML 185
NLRRIST+EILSTHR+QML+ RL+EVKSLI RLA E Q+ +M N FFDLT+NVMLRM+
Sbjct: 123 NLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLRMI 182
Query: 186 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 245
VGKRFYGEDV+D DEA FR+LQ QLGGK+ + DFIP +SW+G G E +I+ECH
Sbjct: 183 VGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMECHQ 242
Query: 246 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 305
KRD FMQNLI+QHK R ++ N+ +DGR+KT+IEVLLELQ+ +PDQY DETIRALML
Sbjct: 243 KRDAFMQNLINQHKNRAIETEPDNSF-RDGRKKTLIEVLLELQESDPDQYNDETIRALML 302
Query: 306 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 365
VLLAAGTETS+ MEWALSLMLN+P+ L+K Q EID +VGHDRL+DESDMAN+P LRGII
Sbjct: 303 VLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGII 362
Query: 366 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 425
NETLRMYPPAPL+VPHESSKDC++GGYHIPRGT+LFVNLWAIHNDPKIW +PRKF+P+RF
Sbjct: 363 NETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDRF 422
Query: 426 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVA 485
+E+EK GF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCFEWERPGEELVDMTEGVA
Sbjct: 423 VKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGVA 482
Query: 486 LTMPKAHCLQAKCTPRPIVHRLHLSHKESN 516
++MPKA LQAKC PRPIV RLHLS +SN
Sbjct: 483 VSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511
BLAST of CSPI04G24600 vs. NCBI nr
Match:
XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 793.9 bits (2049), Expect = 8.4e-226
Identity = 381/510 (74.71%), Postives = 441/510 (86.47%), Query Frame = 0
Query: 6 SIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGP 65
+I +YIPLF VLY L E IHKIRNLPPTPF LP+IGHLHLLK P+YRTLAEISN YGP
Sbjct: 3 AIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRYGP 62
Query: 66 VVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWR 125
VV+ RFG R VLVVSSP AAEECL+KND+VFA+RP L+ISKWFGYNN NLVWSSYGDHWR
Sbjct: 63 VVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDHWR 122
Query: 126 NLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNVMLRML 185
NLRRIST+EILSTHR+QML+ RL+EVKSLI RLA E Q+ +M N FFDLT+NVMLRM+
Sbjct: 123 NLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLRMI 182
Query: 186 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 245
VGKRFYGEDV+D DEA FR+LQ QLGGK+ + DFIP +SW+G G E +I+ECH
Sbjct: 183 VGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMECHQ 242
Query: 246 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 305
KRD FMQNLI+QHK R + + + +N+ +DGR+KT+IEVLLELQ+ +PDQY DETIRALML
Sbjct: 243 KRDAFMQNLINQHKNRAI-ETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALML 302
Query: 306 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 365
VLLAAGTETS+ MEWALSLMLN+P+ L+K Q EID +VGHDRL+DESDMAN+P LRGII
Sbjct: 303 VLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGII 362
Query: 366 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 425
NETLRMYPPAPL+VPHESSKDC++GGYHIPRGT+LFVNLWAIHNDPKIW +PRKF+P+RF
Sbjct: 363 NETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDRF 422
Query: 426 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVA 485
+++EK GF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCFEWERPGE+LVDMTEGVA
Sbjct: 423 VKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGVA 482
Query: 486 LTMPKAHCLQAKCTPRPIVHRLHLSHKESN 516
++MPKA LQAKC PRPIV RLHLS +SN
Sbjct: 483 VSMPKARHLQAKCKPRPIVDRLHLSQTKSN 511
BLAST of CSPI04G24600 vs. NCBI nr
Match:
XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 785.8 bits (2028), Expect = 2.3e-223
Identity = 377/510 (73.92%), Postives = 438/510 (85.88%), Query Frame = 0
Query: 6 SIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYGP 65
+I VYIPLF VLY L E +H+IRNLPPTPF LP+IGHLHLLK P+YRTLAEISN YGP
Sbjct: 3 AIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRYGP 62
Query: 66 VVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWR 125
VV+ RFG R VLVVSSP AAEECL+KND+VFA+RP L+ISKWFGYNN NLVWSSYGDHWR
Sbjct: 63 VVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDHWR 122
Query: 126 NLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIHNMKNEFFDLTYNVMLRML 185
NLRRIST+EILSTHR+QML+ R +EVKSLI RLA E Q+ +M N FFDLT+NVMLRM+
Sbjct: 123 NLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLRMI 182
Query: 186 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 245
VGKRFYGEDV+D DEA FR+LQ QLGGK+ ++DFIP +SW+G G E +I+ECH
Sbjct: 183 VGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEIMECHQ 242
Query: 246 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 305
KRD FMQNLI+QHK R ++ N+ +DGR+KT+IEVL+ELQ+ +PDQY DETIRALML
Sbjct: 243 KRDAFMQNLINQHKNRAIETEPDNSF-RDGRKKTLIEVLVELQESDPDQYNDETIRALML 302
Query: 306 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 365
VLLAAGTETS+ MEWALSLMLN+P+ L+K Q EID +VGHDRL+DESDMAN+P LRGII
Sbjct: 303 VLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGII 362
Query: 366 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 425
NETLRMYPPAPL+VPHESSKDC++GGY IPRGT+LFVNLWAIHNDPKIW +PRKF+P+RF
Sbjct: 363 NETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPRKFDPDRF 422
Query: 426 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTEGVA 485
+++EK GF L+PFGSGRRGCPGEGL LRVIGLVLG+L+QCFEWERPGE+LVDMTEGVA
Sbjct: 423 VKVDSEKHGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGVA 482
Query: 486 LTMPKAHCLQAKCTPRPIVHRLHLSHKESN 516
++MPKA LQAKC PRPIV RLHLS +SN
Sbjct: 483 VSMPKARHLQAKCKPRPIVARLHLSQTKSN 511
BLAST of CSPI04G24600 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 453.8 bits (1166), Expect = 1.9e-127
Identity = 245/514 (47.67%), Postives = 327/514 (63.62%), Query Frame = 0
Query: 3 VLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS-- 62
VL SIF L F++ + ++L K +NLPP+P LP+IGHL LLK PI+RTL S
Sbjct: 9 VLYSIF---SLIFLIIS-FKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSET 68
Query: 63 ---NHYGPVVYFRFGQRRVLVVSS-PFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLV 122
N G V+ R G R V VVSS AAEEC KND+V ANRP +II K GYNNTN++
Sbjct: 69 LDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMI 128
Query: 123 WSSYGDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLI---RRLANNEKQIHNMKNEF 182
+ YGDHWRNLRR+ TIEI STHR+ R +EV+ LI RLA +K + +K
Sbjct: 129 AAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPML 188
Query: 183 FDLTYNVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFG 242
DLT+N ++RM+ GKR+YGE+ D +EAK R+L D+G D++P +
Sbjct: 189 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--- 248
Query: 243 STIENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPD 302
S+ EN++ + + D F+Q LID + Q TMI+ LL LQ+ + +
Sbjct: 249 SSYENRVKKLGEETDKFLQGLIDDKR------------GQQETGTTMIDHLLVLQKSDIE 308
Query: 303 QYTDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDES 362
YTD+ I+ ++L+++ AGT TS +EWALS +LNHP + K ++EIDN+VG DRLI+E+
Sbjct: 309 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEA 368
Query: 363 DMANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKI 422
D++ LP L+ I+ ETLR++P PL+VPH +S+DC IG Y +PRGT L VN WAIH DP
Sbjct: 369 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 428
Query: 423 WDNPRKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERP 482
WD+P F P RFE E + L+ FG GRR CPG GLA R++GL LG+L+QCFEWER
Sbjct: 429 WDDPDSFKPERFEKEEEAQ---KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERV 488
Query: 483 GEELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
G VDM EGV T+PKA L+A C RP +H++
Sbjct: 489 GNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKI 500
BLAST of CSPI04G24600 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 446.4 bits (1147), Expect = 3.1e-125
Identity = 240/512 (46.88%), Postives = 331/512 (64.65%), Query Frame = 0
Query: 4 LESIFVYIPLFFVLYTLIEYLIHKIR---NLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 63
+E+ + + FV+ +LI YLI K++ NLPP+P LPVIGHL LLK PI+RT +S
Sbjct: 1 METKTLIFSILFVVLSLI-YLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLS 60
Query: 64 N--HYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWS 123
+ P+ R G R V V SS AEEC TKND+V ANRP I++K Y+ T ++ +
Sbjct: 61 QSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAA 120
Query: 124 SYGDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIH---NMKNEFFD 183
SYGDHWRNLRRI ++EI S HR+ R +E++ L+ RL+ N Q +MK+ D
Sbjct: 121 SYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSD 180
Query: 184 LTYNVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGST 243
LT+N +LRM+ GKR+YG+ V+D EAK RQL D+ G D++P + + S
Sbjct: 181 LTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV---SD 240
Query: 244 IENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQY 303
E ++ + + D F+Q L+D+ ++ + K N TMI+ LL LQ+ PD +
Sbjct: 241 YETRVKKLAGRLDEFLQGLVDEKREA---KEKGN---------TMIDHLLTLQESQPDYF 300
Query: 304 TDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDM 363
TD I+ ML L+ AGT+TS +EWALS +LNHP L K ++EID ++G DRL+DESD+
Sbjct: 301 TDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDI 360
Query: 364 ANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWD 423
+NLP L+ I++ETLR+YP AP+++PH +S+DC + GY +PRGTIL N+WAIH DP++WD
Sbjct: 361 SNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWD 420
Query: 424 NPRKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGE 483
+P F P RF E E L+PFG GRR CPG GLA R+I L LG+L+QC EWE+ GE
Sbjct: 421 DPMSFKPERF---EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE 480
Query: 484 ELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
E VDM+EG +TMPKA L+A C RP V ++
Sbjct: 481 E-VDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492
BLAST of CSPI04G24600 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 230/512 (44.92%), Postives = 322/512 (62.89%), Query Frame = 0
Query: 4 LESIFVYIPLFFVLYTLIEYLIHKIR---NLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 63
+E++ + F++ +LI +LI +I+ NLPP+P LPVIGHL LLK P++R +S
Sbjct: 1 MEALMLIFTFCFIVLSLI-FLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60
Query: 64 NHYG--PVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWS 123
G P++ R G R + VVSS AEEC TKND++ ANR I +K Y N+ +V +
Sbjct: 61 QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120
Query: 124 SYGDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIH---NMKNEFFD 183
SY +HWRNLRRI +EI S HR+ S R +E++ LI RL N MK+ F D
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSD 180
Query: 184 LTYNVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGST 243
LT+N ++RML GK +YG+ +D EAK R L + G D+IP ++W+ +
Sbjct: 181 LTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-- 240
Query: 244 IENKIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQY 303
E +I + + D F+Q L+D+ ++ ++ + TM++ LL LQ+ P+ Y
Sbjct: 241 -ETRIKKLAGRLDEFLQGLVDEKRE-----------GKEKKENTMVDHLLCLQETQPEYY 300
Query: 304 TDETIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDM 363
D I+ ML L+A GT+T+ +EWALS +LN+P+ L K ++EID +G DRL++ESD+
Sbjct: 301 MDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDI 360
Query: 364 ANLPSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWD 423
NLP L+ I++ETLR+YP AP+++PH +SKDC +GGY +PRGT+L N WAIH DP +WD
Sbjct: 361 PNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWD 420
Query: 424 NPRKFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGE 483
+P F P RFE K L+PFG GRR CPG GLA R++ L LG+L+QCFEWER GE
Sbjct: 421 DPTSFKPERFEKEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE 480
Query: 484 ELVDMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
E VDMTEG LTMPKA L+A C R V ++
Sbjct: 481 EEVDMTEGPGLTMPKARPLEAMCRARDFVGKI 494
BLAST of CSPI04G24600 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 438.7 bits (1127), Expect = 6.4e-123
Identity = 238/509 (46.76%), Postives = 317/509 (62.28%), Query Frame = 0
Query: 9 VYIPLFFVLYTLIEYLIHKIR---NLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYG- 68
+ I F L+TL +L+ + + NLPP+P LPVIGHLHLLK P++RT +S G
Sbjct: 6 ILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGN 65
Query: 69 -PVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDH 128
PV + R G R V V+SS AEEC TKND+V ANRP ISK GYN T L+ +SYGDH
Sbjct: 66 APVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDH 125
Query: 129 WRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNEKQIH-----NMKNEFFDLTY 188
WRNLRRI+ +EI STHR+ R +E++ LI L+ + +H MK +L
Sbjct: 126 WRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRD--SLHGFVEVEMKTLLTNLAS 185
Query: 189 NVMLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIEN 248
N +RML GKR++GED DD AK+ + L + G D++ + W+ S+ E
Sbjct: 186 NTTIRMLAGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEK 245
Query: 249 KIIECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDE 308
+I + DTF+Q L+D+ + ++ + +TMI+ LL LQ PD YTD
Sbjct: 246 RIKNLGNRFDTFLQKLVDEKR------------AEKEKGETMIDHLLALQDIQPDYYTDV 305
Query: 309 TIRALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANL 368
I+ ++L L+ AGT+TS +EWA+S +LNHP+ LKK + EID +VG DRL+DESD+ NL
Sbjct: 306 IIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNL 365
Query: 369 PSLRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPR 428
L+ I+ ETLRMYP PL++PH SS+DC +GGY IP GT++ N WA+H DP++W++P
Sbjct: 366 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 425
Query: 429 KFNPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELV 488
F P RF E E LI FG GRR CPG GLA R+I LG+LVQCFEWER GE+ V
Sbjct: 426 IFKPERF---EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFV 485
Query: 489 DMTEGVALTMPKAHCLQAKCTPRPIVHRL 508
DMTE T+PKA L+A C R IV +L
Sbjct: 486 DMTEDKGATLPKAIPLRAMCKARSIVDKL 491
BLAST of CSPI04G24600 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 437.6 bits (1124), Expect = 1.4e-122
Identity = 230/503 (45.73%), Postives = 320/503 (63.62%), Query Frame = 0
Query: 14 FFVLYTLIEYLIHKIR---NLPPTPFPLLPVIGHLHLLKNPIYRTLAEISNHYG--PVVY 73
F + + ++I +I+ NLPP+P LPVIGHL LLK P++R +S G P++
Sbjct: 10 FLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIIS 69
Query: 74 FRFGQRRVLVVSSPFAAEECLTKNDIVFANRPCLIISKWFGYNNTNLVWSSYGDHWRNLR 133
R G R V VVSS AEEC TKND+V ANR + SK Y T +V +SYGDHWRNLR
Sbjct: 70 LRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129
Query: 134 RISTIEILSTHRIQMLSMFRLEEVKSLIRRLANN---EKQIHNMKNEFFDLTYNVMLRML 193
RI +EI S HR+ S R +E+ LI L+ N E MK+ F +LT+N ++RML
Sbjct: 130 RIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRML 189
Query: 194 VGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKIIECHV 253
GK +YG+ +D EAK R+L + G D++P ++W+ + E +I +
Sbjct: 190 AGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKKIAS 249
Query: 254 KRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETIRALML 313
+ D F+Q L+D+ ++ ++ R+ TM++ LL LQ+ P+ YTD I+ +ML
Sbjct: 250 RLDEFLQGLVDERRE-----------GKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIML 309
Query: 314 VLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPSLRGII 373
L+ AGT+TS +EW LS +LNHP+ L K ++EIDN+VG +RL++ESD+++LP L+ I+
Sbjct: 310 SLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIV 369
Query: 374 NETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKFNPNRF 433
+E+LR+YP +PL+VPH +S+DC +GGYH+PRGT+L N WAIH DPKIWD+P F P RF
Sbjct: 370 SESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF 429
Query: 434 ESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDMTE-GV 493
E E L+ FG GRR CPG GLA R+ L +G+L+QCFEWER GEE VDMTE G
Sbjct: 430 ---EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGG 489
Query: 494 ALTMPKAHCLQAKCTPRPIVHRL 508
+ MPKA L A C RP+V ++
Sbjct: 490 GVIMPKAIPLVAMCKARPVVGKI 495
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 8.6e-133 | 50.41 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q9FG65 | 2.7e-126 | 47.67 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Q6WNQ8 | 2.0e-121 | 46.18 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q9LHA1 | 3.8e-120 | 45.05 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
P93147 | 3.2e-119 | 42.88 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0L2I7 | 7.0e-303 | 99.61 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1 | [more] |
A0A1S3BUR6 | 5.4e-279 | 90.50 | cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1 | [more] |
A0A6J1FJC9 | 8.2e-227 | 75.10 | cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459... | [more] |
A0A6J1J228 | 4.1e-226 | 74.71 | cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119... | [more] |
A0A6J1CYI5 | 4.4e-212 | 70.04 | cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV... | [more] |
Match Name | E-value | Identity | Description | |
XP_004141397.1 | 1.4e-302 | 99.61 | cytochrome P450 81Q32 [Cucumis sativus] >KGN55249.1 hypothetical protein Csa_012... | [more] |
XP_008453068.1 | 1.1e-278 | 90.50 | PREDICTED: cytochrome P450 81D1-like [Cucumis melo] | [more] |
XP_022940352.1 | 1.7e-226 | 75.10 | cytochrome P450 81D1-like isoform X1 [Cucurbita moschata] | [more] |
XP_022982225.1 | 8.4e-226 | 74.71 | cytochrome P450 81D1-like isoform X1 [Cucurbita maxima] | [more] |
XP_023523281.1 | 2.3e-223 | 73.92 | cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
AT5G36220.1 | 1.9e-127 | 47.67 | cytochrome p450 81d1 | [more] |
AT4G37370.1 | 3.1e-125 | 46.88 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37360.1 | 2.6e-124 | 44.92 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37330.1 | 6.4e-123 | 46.76 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT4G37340.1 | 1.4e-122 | 45.73 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |