CSPI04G24160 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G24160
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr4: 22024621 .. 22027914 (-)
RNA-Seq ExpressionCSPI04G24160
SyntenyCSPI04G24160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTGATTGAAAATGAATTTAGTAAAAATTATGTGATATTGAAGCCAGAAAATGCTAATTTATTGGATTTGTTTCTGTTCTTACTTCCATTTGGATTAAGAAAGAAAAAATTCATTGATTGTCCATATGGCAAAGAAGATTATTTCACAAACTTCACAGACCGATGGCTAATTGTCGTCTCCATGTTGCTTCAGTTCATCCTATCCCTCATTGCCACTCCTCTTGCAAAATTAGACGCATTTCTTCTTAAACTCTTCAACTTTATTTCTTTCAACGGCGGCTTCTTGGGGATTCTCTCCAAAATTCTCAGAGGTATATATTATTAATTATTATGATTACATTTTAAATATTAACTAACAAGGGTTTCTTTTTTTTGTTTTGTTTGTGATGTAATAATTAATTATTTTGTATAGGAAAGGGTTTTGTGAAGGTTAATGAAGATTCGGCTGAGTACACATCGGTGGTGGGATTTGCAGATTGGAGAAGAGATTTGGATTCGTCAATAAAACCTGAACAGAGTTTCAGATATTACAGTGCTTTAACTGCAATGGCTACCAAAATCTCTTATGAATCCAAACCATTTGTCCAATCCGTTGTAAATGATCGTTGGAAGGTAAAACTTATATTCCTTTAAATAGGAACAGACATACTAGATCGAAAATTCGAGCCAATCCTTCACCAAAATTTCATAATTAATTACTTAACTCCTTTACAATATAATCGTTATGTCTTAATAACTATTTGAATTTGAATAATCACTTGCATCTCTCCATCTGAAAAATTATTAGTCATACTATGACGATCCAGTCATAGTAGTAATTATACACATCTAGAAATAAAGTTTGACTCTACCAAACAACAAATGTAAAGATTGGTAGAAGAAATTAACAATATATAATAGAAATAATTAAATTTTGAAAGTTGGATTAAAATATACAAAAAAAAAATTCATATTAGAGATGTAAACTAAAATTTGATTATAAATCAATATTAGAAGGGTGTGGTAAATGATTGTTTTTCTTGTCTTTTTTTCCCTCCCTCTTGCAGATGAAGTTGCTGGGTTACTTCAACTTTTGGAATGGTAAGAGTTTTTGAGCTCTGCATCAAAGGTCATAATTAGATTAGTTAATCAACTTTGAATTAGTTAGAATTAATTGATTGGGTCTTAAAGCTGACCAGTTCAAGTTTATTAATACTCTATTAATTAAGTACATGTTGGTTTAGTGGAGGAATATTAATGAAGTGACCATAGAATAAAAACAGTGAAAGAGCCGTGAGGTTTATACCATTGTATGGTGTAAGAACAGTGACAGGAAGGCCTAATTTTTAACTTTATTGGACAAATAACGTAACCTTCACAAGGTTATTTGTCTTCAACCAAACTTAGGCTGTCCTTAAGTTTCTCCACTCATCTTGGACATCTTTTTTTTTCTTTTTTTTTTCTTTCATTTTTTGAAAATGGAGTAACTGTCTGATTAATAAGAAGAATTAAATTTTGTTATTCAGTTTTACATTTTGAAAATCTTCTTTGTCGTTTTTAATTTTGATTTTGATTTTTAGCTAGAGGATTTTATTCTTCAAACTATAAATATAAAGAAAATGTGAAATTGAAAATAACTCAACCTAGATAAGGCTTGCATCAATTATAATTTTGAAGTCAAATGGTTGAAATAGTTTAATTAATTGAACATAGTTTTAATAAGAAAAATAAATCTGTTTAATAAAAGTAAGAAGGATAATATATATATATATATAAATATATATATAGAATATAGTAAATAATAATATTGAACTTTTATGTATGAAATTGTTGTTGTTCAGATTTTCAAGGTAGAACTACGACTCAGGCGTTTATGTTTAAGAACACGGCCATAGATCCGAATGTAACAATTGTTGCATTTAGAGGCACCTCCCCACTTGACGCATACGATTGGCAAGTGGATTTTGATTTCTCTTGGTACGCTTACATTACATTCTTTCATTTCTTTTAATTTAATTACGGCCTTATTCATTATTTAATCTCAAAGTTTAGTTTTCTTCTTTAAAAGAATTATGTTTTAAGATTAGTATGATTCACCAAAGTGAATTTGATTAGTGGTTTTAGCTTGTTTGGAGCTCACCATCTTTGGATGAGTAACTAATACCTCTTATAATCATTTCTATTAAACCCAAAACGTGTTTTGTGAATCTAATATTTACAGGTACGAAATCGAGGATGTCGGCCTGATCCACAGTGGGTTCATGAAAGCCCTAGGGCTTCAGAAGGCAACAGGATGGCCAAAGGAGCTTCCCAAAACTCAAACACATGAGTTCGCATATTACACCCTCAGGAAACAACTAAGAGACATTGCCAAAGCCAACGAGAAGGCAAGGTTCATATTCACTGGCCACAGTTTAGGTGGAGCTTTGGCCACATTATTTGTCACTGTGTTGTGCCTACATGATGAGTCAACCATTTTGGAGAAATTGGATAGCGTTTACACATATGGGCAACCAAGAGTTGGTGACCAACAATTTGCAAAGTTCATGCTTAATTGTATCCAAAAATATGGGTTCAAGTATCATAGATATGTTTACTCTTATGACTTGGTGCCCAGAGTTCCTTCTGATGCCGTCCTTTTCAAATATAAACACTTCGGTAGATGTGTCTACTTCAACAGCCTTTACAAAGGAAGGGTAAGAGAACAAATTAACACCACCTTTTCTTTTTTCCTTTTTATCACTCAATAAATTACTCTATAATGCTCATCGCCACCTAAGAAGCATTGATAGATACGATTTACATATCTTTTCTTTCTCGAAAATTTATAGCAGCATACACTTTTTTAAAAAAATATATATATATTGTTGGTTGGGTGCAGATTGTGAAAGAGCAACCAAACAAGAACTACTTCTCGTTGCTATGGGTGATACCCAAATATTTAAGTGCATGGTGGGAGCTAATTAGGAGTTTCATAATCCCATTTGTGAAGGGTTTTGATTACTACGAGAGTTTGTTAATGAAGGGTGCAAGGTTGGTTGGACTCGTGATTCCAGGACTTACTGCTCATTTTCCTTTGAACTATGTTAATGTCACTCGTTTGGGTAAGTTAAATGTACCCGACGAGGTCGAAGATCCCATTCTTCGATACGAAATTGAAGACGACTTCTAAAAATGTTCAAGTACTTGTCATTTATTGTTGAACGACTAAGTTAATTACCTTAGTTGTACTTTATGCAACGTGTAGCTCACAACTATGTAACGTGTAGCTCATTTTCAATACCTTTTTCTAGATGATTTAAGTGTGGAG

mRNA sequence

ATGGTTTTGATTGAAAATGAATTTAGTAAAAATTATGTGATATTGAAGCCAGAAAATGCTAATTTATTGGATTTGTTTCTGTTCTTACTTCCATTTGGATTAAGAAAGAAAAAATTCATTGATTGTCCATATGGCAAAGAAGATTATTTCACAAACTTCACAGACCGATGGCTAATTGTCGTCTCCATGTTGCTTCAGTTCATCCTATCCCTCATTGCCACTCCTCTTGCAAAATTAGACGCATTTCTTCTTAAACTCTTCAACTTTATTTCTTTCAACGGCGGCTTCTTGGGGATTCTCTCCAAAATTCTCAGAGGAAAGGGTTTTGTGAAGGTTAATGAAGATTCGGCTGAGTACACATCGGTGGTGGGATTTGCAGATTGGAGAAGAGATTTGGATTCGTCAATAAAACCTGAACAGAGTTTCAGATATTACAGTGCTTTAACTGCAATGGCTACCAAAATCTCTTATGAATCCAAACCATTTGTCCAATCCGTTGTAAATGATCGTTGGAAGATGAAGTTGCTGGGTTACTTCAACTTTTGGAATGATTTTCAAGGTAGAACTACGACTCAGGCGTTTATGTTTAAGAACACGGCCATAGATCCGAATGTAACAATTGTTGCATTTAGAGGCACCTCCCCACTTGACGCATACGATTGGCAAGTGGATTTTGATTTCTCTTGGTACGAAATCGAGGATGTCGGCCTGATCCACAGTGGGTTCATGAAAGCCCTAGGGCTTCAGAAGGCAACAGGATGGCCAAAGGAGCTTCCCAAAACTCAAACACATGAGTTCGCATATTACACCCTCAGGAAACAACTAAGAGACATTGCCAAAGCCAACGAGAAGGCAAGGTTCATATTCACTGGCCACAGTTTAGGTGGAGCTTTGGCCACATTATTTGTCACTGTGTTGTGCCTACATGATGAGTCAACCATTTTGGAGAAATTGGATAGCGTTTACACATATGGGCAACCAAGAGTTGGTGACCAACAATTTGCAAAGTTCATGCTTAATTGTATCCAAAAATATGGGTTCAAGTATCATAGATATGTTTACTCTTATGACTTGGTGCCCAGAGTTCCTTCTGATGCCGTCCTTTTCAAATATAAACACTTCGGTAGATGTGTCTACTTCAACAGCCTTTACAAAGGAAGGATTGTGAAAGAGCAACCAAACAAGAACTACTTCTCGTTGCTATGGGTGATACCCAAATATTTAAGTGCATGGTGGGAGCTAATTAGGAGTTTCATAATCCCATTTGTGAAGGGTTTTGATTACTACGAGAGTTTGTTAATGAAGGGTGCAAGGTTGGTTGGACTCGTGATTCCAGGACTTACTGCTCATTTTCCTTTGAACTATGTTAATGTCACTCGTTTGGGTAAGTTAAATGTACCCGACGAGGTCGAAGATCCCATTCTTCGATACGAAATTGAAGACGACTTCTAAAAATGTTCAAGTACTTGTCATTTATTGTTGAACGACTAAGTTAATTACCTTAGTTGTACTTTATGCAACGTGTAGCTCACAACTATGTAACGTGTAGCTCATTTTCAATACCTTTTTCTAGATGATTTAAGTGTGGAG

Coding sequence (CDS)

ATGGTTTTGATTGAAAATGAATTTAGTAAAAATTATGTGATATTGAAGCCAGAAAATGCTAATTTATTGGATTTGTTTCTGTTCTTACTTCCATTTGGATTAAGAAAGAAAAAATTCATTGATTGTCCATATGGCAAAGAAGATTATTTCACAAACTTCACAGACCGATGGCTAATTGTCGTCTCCATGTTGCTTCAGTTCATCCTATCCCTCATTGCCACTCCTCTTGCAAAATTAGACGCATTTCTTCTTAAACTCTTCAACTTTATTTCTTTCAACGGCGGCTTCTTGGGGATTCTCTCCAAAATTCTCAGAGGAAAGGGTTTTGTGAAGGTTAATGAAGATTCGGCTGAGTACACATCGGTGGTGGGATTTGCAGATTGGAGAAGAGATTTGGATTCGTCAATAAAACCTGAACAGAGTTTCAGATATTACAGTGCTTTAACTGCAATGGCTACCAAAATCTCTTATGAATCCAAACCATTTGTCCAATCCGTTGTAAATGATCGTTGGAAGATGAAGTTGCTGGGTTACTTCAACTTTTGGAATGATTTTCAAGGTAGAACTACGACTCAGGCGTTTATGTTTAAGAACACGGCCATAGATCCGAATGTAACAATTGTTGCATTTAGAGGCACCTCCCCACTTGACGCATACGATTGGCAAGTGGATTTTGATTTCTCTTGGTACGAAATCGAGGATGTCGGCCTGATCCACAGTGGGTTCATGAAAGCCCTAGGGCTTCAGAAGGCAACAGGATGGCCAAAGGAGCTTCCCAAAACTCAAACACATGAGTTCGCATATTACACCCTCAGGAAACAACTAAGAGACATTGCCAAAGCCAACGAGAAGGCAAGGTTCATATTCACTGGCCACAGTTTAGGTGGAGCTTTGGCCACATTATTTGTCACTGTGTTGTGCCTACATGATGAGTCAACCATTTTGGAGAAATTGGATAGCGTTTACACATATGGGCAACCAAGAGTTGGTGACCAACAATTTGCAAAGTTCATGCTTAATTGTATCCAAAAATATGGGTTCAAGTATCATAGATATGTTTACTCTTATGACTTGGTGCCCAGAGTTCCTTCTGATGCCGTCCTTTTCAAATATAAACACTTCGGTAGATGTGTCTACTTCAACAGCCTTTACAAAGGAAGGATTGTGAAAGAGCAACCAAACAAGAACTACTTCTCGTTGCTATGGGTGATACCCAAATATTTAAGTGCATGGTGGGAGCTAATTAGGAGTTTCATAATCCCATTTGTGAAGGGTTTTGATTACTACGAGAGTTTGTTAATGAAGGGTGCAAGGTTGGTTGGACTCGTGATTCCAGGACTTACTGCTCATTTTCCTTTGAACTATGTTAATGTCACTCGTTTGGGTAAGTTAAATGTACCCGACGAGGTCGAAGATCCCATTCTTCGATACGAAATTGAAGACGACTTCTAA

Protein sequence

MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIVVSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIEDDF*
Homology
BLAST of CSPI04G24160 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.3e-85
Identity = 182/480 (37.92%), Postives = 263/480 (54.79%), Query Frame = 0

Query: 56  RWLIVVSMLLQFILSLIATPLAKLDAFLLKLF-NFISFNGGFLGILSKILRGKGFVKVNE 115
           RW+I VS++++ ++++   P+ +   +L++ F N  S NG FLG+L  IL GK  V  + 
Sbjct: 86  RWVIFVSIIVRKLIAIFGKPM-EWTGYLVEFFLNLFSLNGNFLGLLYNILHGK-VVMPHR 145

Query: 116 DSAEYTSVVGFADWRRDL-------------DSSIKPEQSFRYYSALTAMATKISYESKP 175
            S  + S +G  D R +L             D   K     R    L  MA+K++YE++ 
Sbjct: 146 GSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEK 205

Query: 176 FVQSVVNDRWKMKLLGYFNFWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDW 235
            V++VVN  WKM  + ++N WNDF+   +TQ F+  +   D N+ +V+FRGT P DA DW
Sbjct: 206 VVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDW 265

Query: 236 QVDFDFSWYEIEDVGLIHSGFMKALGLQK-------------------------ATGWPK 295
             DFD+SWYEI  +G +H GF++ALGL                           AT  P 
Sbjct: 266 ITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPS 325

Query: 296 ELPKTQT---------------------------------HEFAYYTLRKQLRDIAKANE 355
           E  K+ T                                    AYY +R +L+ + K ++
Sbjct: 326 ESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHK 385

Query: 356 KARFIFTGHSLGGALATLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQ 415
            A+F+ TGHSLGGALA LF  VL LH+E  ++E+L  +YTYGQPRVG++Q  +FM   ++
Sbjct: 386 NAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLE 445

Query: 416 KYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWV 464
               KY R VY  DLVPR+P D   F +KHFG C Y+NSLY  + + E+PN NYF + ++
Sbjct: 446 HPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFL 505

BLAST of CSPI04G24160 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-83
Identity = 182/500 (36.40%), Postives = 278/500 (55.60%), Query Frame = 0

Query: 11  NYVILKPENANLLDLFLFLLPFGLR-----KKKFIDCPYGKEDYFTNFTD-----RWLIV 70
           NY+I++P     +DLF     +G+R     K KF++ P  +E       +     RW+IV
Sbjct: 18  NYLIVRPHRGGYIDLF----RYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIV 77

Query: 71  VSMLLQFILSLIATPLAKLDAFLLKLF-NFISFNGGFLGILSKILRGKGFVKVNEDSAEY 130
           VS+L++ I+ L+ TP+ +   F++  F N  S NGGF G+L ++++ K  V     S  +
Sbjct: 78  VSILVRKIIRLLRTPM-EFTGFVVDFFLNLFSANGGFFGLLLRLIQAK-VVIPERGSVTF 137

Query: 131 TSVVGFADWRRDL-----------------DSSIKPEQSFRYYSALTAMATKISYESKPF 190
            S +G  D R  L                    +K E   R    L  MA+K++YE+   
Sbjct: 138 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 197

Query: 191 VQSVVNDRWKMKLLGYFNFWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQ 250
           V++VV+  WKM L+ + + WND+Q + +TQ F+F +   D N+ +++FRGT P DA DW 
Sbjct: 198 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 257

Query: 251 VDFDFSWYEIEDVGLIHSGFMKALGLQ-------------KATGWPKELPK----TQTHE 310
            DFD+SWYE+ +VG +H GF++A+GL              + T   +E  K         
Sbjct: 258 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 317

Query: 311 FAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLCLHDESTILEKLDSVYTYG 370
            AYY +R  L+ +   +E ARF+ TGHSLGGALA LF T+L L++E+ I+++L  VYT+G
Sbjct: 318 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 377

Query: 371 QPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFGRCVYFNSLYK 430
           QPR+G+++   FM   + +   +Y R VY  D+VPR+P D   F YKHFG C++++S Y 
Sbjct: 378 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 437

Query: 431 GRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVKGFDYYESLLMKGARLVGLVIP 466
               +++P+ N + L + I  ++ A WEL+R   + +  G DY E       RL+GLVIP
Sbjct: 438 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 497

BLAST of CSPI04G24160 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.9e-75
Identity = 174/506 (34.39%), Postives = 259/506 (51.19%), Query Frame = 0

Query: 9   SKNYVILKPENANLLDLFLFLLPFGLRK-KKFIDCPYGKEDYFTNFTDRWLIVVSMLLQF 68
           S +++I+ P+    LDLF  ++   L    KF      +      +  RW   VS+ +  
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRW--PVSVFICK 68

Query: 69  ILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKV-NEDSAEYTSVVGFA 128
           IL L   P A L      L NF   N GFLGIL  I   +  +K+     A++ S +G+ 
Sbjct: 69  ILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIR--LKIPKRGEADFISTIGYL 128

Query: 129 DWRRDL--------------------------DSSIKPEQSFRYYSALTAMATKISYESK 188
           D R DL                          +S IK     R    L  MA+K++YE+ 
Sbjct: 129 DSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYENT 188

Query: 189 PFVQSVVNDRWKMKLLGYFNFWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYD 248
             V+ VV + WKM  +  +   N FQ    T AF+F +   D N+ +++FRGT P    +
Sbjct: 189 KVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPN 248

Query: 249 WQVDFDFSWYEIEDVGLIHSGFMKALGL--------------QKATGWPKELPKTQ---- 308
           W  DFDFS   + D G +H GF++A+GL               K+ G   EL K      
Sbjct: 249 WCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAP 308

Query: 309 -----THEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLCLHDESTILEK 368
                 ++  Y+     L+ + K ++ A+F+ TGHSLGGALA LF  +L +  E+ +L++
Sbjct: 309 DHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDR 368

Query: 369 LDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFGRC 428
           L +VYT+GQPR+G+     FM N +     +Y R VY  D+VPRVP D V F ++HFG C
Sbjct: 369 LLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGTC 428

Query: 429 VYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVKGFDYYESLLMKGA 464
           +Y++S + G   KE+P++N F +   I  +++AWWEL RSFI+  V G +Y E+   +  
Sbjct: 429 IYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRMF 488

BLAST of CSPI04G24160 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-09
Identity = 52/167 (31.14%), Postives = 84/167 (50.30%), Query Frame = 0

Query: 207 IVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKALGLQKATGWPKELPKTQTHEF 266
           +++FRG+  ++  +W  + +F   EI D+           G +   G+      T +   
Sbjct: 99  VLSFRGSRSIE--NWIGNLNFDLKEINDI---------CSGCRGHDGF------TSSWRS 158

Query: 267 AYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLCLHDESTILEKLDSVYTYGQ 326
              TLR+++ D  + +   R +FTGHSLGGALAT  V    L      ++    V++YG 
Sbjct: 159 VADTLRQKVEDAVREHPDYRVVFTGHSLGGALAT--VAGADLRGNGYDID----VFSYGA 218

Query: 327 PRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKH 374
           PRVG++ FA+F+   +Q  G  Y R  ++ D+VPR+P     F Y H
Sbjct: 219 PRVGNRAFAEFL--TVQTGGTLY-RITHTNDIVPRLPPRE--FGYSH 237

BLAST of CSPI04G24160 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 2.8e-08
Identity = 54/170 (31.76%), Postives = 72/170 (42.35%), Query Frame = 0

Query: 208 VAFRGTSPLDAYDWQVDFDFSWYEIEDVG--LIHSGFMKALGLQKATGWPKELPKTQTHE 267
           + FRG+S +   +W  D  F       V    +H GF+ + G  +      EL  T   +
Sbjct: 171 IVFRGSSSI--RNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQ-----NELVATVLDQ 230

Query: 268 FAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLCLHDESTILEKLDSVYTYG 327
           F  Y   K                TGHSLGGA A L    L   +E      L  +YT G
Sbjct: 231 FKQYPSYK-------------VAVTGHSLGGATALLCALDLYQREEGLSSSNL-FLYTQG 290

Query: 328 QPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFG 376
           QPRVGD  FA ++++     G  Y R V   D+VP +P  A  F + H G
Sbjct: 291 QPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLPPAA--FGFLHAG 313

BLAST of CSPI04G24160 vs. ExPASy TrEMBL
Match: A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.2e-285
Identity = 482/483 (99.79%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
           FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300
           GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT
Sbjct: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300

Query: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360
           LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP
Sbjct: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360

Query: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420
           RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII
Sbjct: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420

Query: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480
           PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE
Sbjct: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480

Query: 481 DDF 484
           DD+
Sbjct: 481 DDY 483

BLAST of CSPI04G24160 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.3e-270
Identity = 456/483 (94.41%), Postives = 467/483 (96.69%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MVL+ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNF DRWLI 
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           VSMLLQFIL+LIATPLAKLDAFLLKLFNFISFNGG LGILSKILRGKGFVKV EDS+EYT
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWRRDLDSSIK E+SFRYYSALT MATKISYESKPFVQSVVNDRWKMKLLGYFN
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
           FWNDFQG+ TTQAF F+NTAIDPNV IVAFRGTSPLDAYDWQVD D SWYEIE VG IHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300
           GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT
Sbjct: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300

Query: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360
           LFVTVL LHDESTILEKLDSVYTYGQPRVGDQQFAKFM+NC+QKYGFKYHRYVYSYDLVP
Sbjct: 301 LFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVP 360

Query: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420
           RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPN+NYFSLLWVIPKYLSAWWELIRSFII
Sbjct: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFII 420

Query: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480
           PFVKGFDYYESLLMKGARLVGL+IPGL+AHFPLNYVNVTRLGKLNVPDEVEDP L YEIE
Sbjct: 421 PFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEIE 480

Query: 481 DDF 484
           DDF
Sbjct: 481 DDF 483

BLAST of CSPI04G24160 vs. ExPASy TrEMBL
Match: A0A5D3D9F3 (Lipase class 3 family protein-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003310 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.5e-233
Identity = 397/421 (94.30%), Postives = 407/421 (96.67%), Query Frame = 0

Query: 63  MLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSV 122
           MLLQFIL+LIATPLAKLDAFLLKLFNFISFNGG LGILSKILRGKGFVKV EDS+EYTSV
Sbjct: 1   MLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSV 60

Query: 123 VGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFW 182
           VGFADWRRDLDSSIK E+SFRYYSALT MATKISYESKPFVQSVVNDRWKMKLLGYFNFW
Sbjct: 61  VGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFW 120

Query: 183 NDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGF 242
           NDFQG+ TTQAF F+NTAIDPNV IVAFRGTSPLDAYDWQVD D SWYEIE VG IHSGF
Sbjct: 121 NDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGF 180

Query: 243 MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF 302
           MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF
Sbjct: 181 MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF 240

Query: 303 VTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRV 362
           VTVL LHDESTILEKLDSVYTYGQPRVGDQQFAKFM+NC+QKYGFKYHRYVYSYDLVPRV
Sbjct: 241 VTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRV 300

Query: 363 PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPF 422
           PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPN+NYFSLLWVIPKYLSAWWELIRSFIIPF
Sbjct: 301 PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPF 360

Query: 423 VKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIEDD 482
           VKGFDYYESLLMKGARLVGL+IPGL+AHFPLNYVNVTRLGKLNVPDEVEDP L YEIEDD
Sbjct: 361 VKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEIEDD 420

Query: 483 F 484
           F
Sbjct: 421 F 421

BLAST of CSPI04G24160 vs. ExPASy TrEMBL
Match: A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.1e-224
Identity = 378/484 (78.10%), Postives = 422/484 (87.19%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MV  E EFSKNY+ILKP +A LLDLFLFLLPFGLRK KFIDCP G ED + NFTDRW I 
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           +S+LLQ IL+  ATPLAKLD+FL+ LFNFISFNGG LG+L+KIL+GKG VK NEDS++YT
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWR DLDSSI    +FRYY  LT MA KI+YESKPFVQSVVNDRWKMKLLG+FN
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAI-DPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIH 240
           FWNDFQG+ TTQAFMF+NTA  DPN+ ++AFRGTSPLD YDWQVD DFSWY+IE VG IH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
           SGFMKALGLQKATGWPKEL K Q H+FAYYTLR++LRDIAKAN KARFI TGHSLGGALA
Sbjct: 241 SGFMKALGLQKATGWPKELTKPQ-HDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALA 300

Query: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360
           TLFVT+L LHDE+ ILEKL  VYTYGQPRVGD++FA+FM+N +Q+YGFKY+RYVYS DLV
Sbjct: 301 TLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLV 360

Query: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420
           PR+PSD V+FKYKHFGR +YFN+LYKGRIVK QPNKNYFSLLWVIPKYLSAWWEL+RSFI
Sbjct: 361 PRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFI 420

Query: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480
            P V GFDYYESLLM  AR++GL+IPGL AHFPLNYVN TRLGKL  PDEVEDPIL  +I
Sbjct: 421 TPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDDI 480

Query: 481 EDDF 484
           E D+
Sbjct: 481 EGDY 483

BLAST of CSPI04G24160 vs. ExPASy TrEMBL
Match: A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.7e-221
Identity = 372/484 (76.86%), Postives = 423/484 (87.40%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MV  + EFSKNY+ILKP +A LLDLFLFLLPFGLRK KFIDCP G E  ++NFTDRW I 
Sbjct: 1   MVSKKCEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEASYSNFTDRWFIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           +S+LLQ IL+  ATPL+KLD+FL+ LFNFISFNGG LG+LSKIL+GKG VK NEDS++YT
Sbjct: 61  ISILLQIILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SV+GFADWR DLDSSI    +FRYY  LT MA KI+YESKPFVQSVVNDRWKMKLLG+FN
Sbjct: 121 SVLGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAI-DPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIH 240
           FWNDFQG+ TTQAFMF+NTA  DPN+ ++AFRGTSPLD  DWQV+ DFSWY+IE +G IH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIH 240

Query: 241 SGFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
           SGFMKALGLQKATGWPKEL K Q H+FAYY+LR++LRDIAKAN KARFI TGHSLGGALA
Sbjct: 241 SGFMKALGLQKATGWPKELTKPQ-HDFAYYSLRQKLRDIAKANAKARFIITGHSLGGALA 300

Query: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360
           TLFVT+L LHDE+TILEKL  VYTYGQPRVGD++FA+FM+N +QKYGFKY+RYVYS DLV
Sbjct: 301 TLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDLV 360

Query: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420
           PR+PSD V+FKYKHFGR +YFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWEL+RSFI
Sbjct: 361 PRIPSDGVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSFI 420

Query: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480
            P + GFD+YESLLM  AR+VGL+IPGL AHFPLNYVN  RLGKL VPDE+EDPIL  +I
Sbjct: 421 TPLMWGFDHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKVPDEIEDPILEDDI 480

Query: 481 EDDF 484
           E ++
Sbjct: 481 EGEY 483

BLAST of CSPI04G24160 vs. NCBI nr
Match: XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])

HSP 1 Score: 989.2 bits (2556), Expect = 1.3e-284
Identity = 482/483 (99.79%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
           FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300
           GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT
Sbjct: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300

Query: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360
           LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP
Sbjct: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360

Query: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420
           RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII
Sbjct: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420

Query: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480
           PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE
Sbjct: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480

Query: 481 DDF 484
           DD+
Sbjct: 481 DDY 483

BLAST of CSPI04G24160 vs. NCBI nr
Match: XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])

HSP 1 Score: 939.9 bits (2428), Expect = 8.8e-270
Identity = 456/483 (94.41%), Postives = 467/483 (96.69%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MVL+ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNF DRWLI 
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           VSMLLQFIL+LIATPLAKLDAFLLKLFNFISFNGG LGILSKILRGKGFVKV EDS+EYT
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWRRDLDSSIK E+SFRYYSALT MATKISYESKPFVQSVVNDRWKMKLLGYFN
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
           FWNDFQG+ TTQAF F+NTAIDPNV IVAFRGTSPLDAYDWQVD D SWYEIE VG IHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300
           GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT
Sbjct: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300

Query: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360
           LFVTVL LHDESTILEKLDSVYTYGQPRVGDQQFAKFM+NC+QKYGFKYHRYVYSYDLVP
Sbjct: 301 LFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVP 360

Query: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420
           RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPN+NYFSLLWVIPKYLSAWWELIRSFII
Sbjct: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFII 420

Query: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480
           PFVKGFDYYESLLMKGARLVGL+IPGL+AHFPLNYVNVTRLGKLNVPDEVEDP L YEIE
Sbjct: 421 PFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEIE 480

Query: 481 DDF 484
           DDF
Sbjct: 481 DDF 483

BLAST of CSPI04G24160 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 861.3 bits (2224), Expect = 4.0e-246
Identity = 412/483 (85.30%), Postives = 447/483 (92.55%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           M L+E+EFSKNYVILKPENANLLDLFLFLLPFGLRK+KFIDCP GKED F NF DRWLI 
Sbjct: 1   MDLMESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           +SMLLQFIL++IATPLAKLD+FLLKL NFISFNGG  GILSKIL+GK F++VNEDS EYT
Sbjct: 61  ISMLLQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWRRDLDSSIKP+ +FRYYSALT MA K+SYESKPFVQSVVNDRWKMKLLGYFN
Sbjct: 121 SVVGFADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
           FWNDFQG+ TTQAFMF+NTAIDPNV +VAFRGTS LD+YDWQVD DFSWYEIE VG IHS
Sbjct: 181 FWNDFQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALAT 300
           GFMKALGLQKATGWPKELPK+Q  EFAYYT+R++LRDIAK N+KARFI TGHSLGGALAT
Sbjct: 241 GFMKALGLQKATGWPKELPKSQLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALAT 300

Query: 301 LFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVP 360
           LFVTVL LHDESTILEKL+ +YTYGQPRVGDQQFAKFM+N +QKYGFKYHRYVYS DLVP
Sbjct: 301 LFVTVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVP 360

Query: 361 RVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFII 420
           R+PSDA+LFKYKHFGRCV+FN+LY+GRIVKEQPNKNYFSL+WVIPKYLSAWWELIRS II
Sbjct: 361 RLPSDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLII 420

Query: 421 PFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIE 480
           P VKGFDYYESLLMKG R+VGL IPGL AHFPLNYVN TRLGKLNVPD+VEDPIL  +IE
Sbjct: 421 PLVKGFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNVPDKVEDPILGDDIE 480

Query: 481 DDF 484
           DDF
Sbjct: 481 DDF 483

BLAST of CSPI04G24160 vs. NCBI nr
Match: KAA0064390.1 (lipase class 3 family protein-like 1 [Cucumis melo var. makuwa] >TYK20196.1 lipase class 3 family protein-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 817.8 bits (2111), Expect = 5.1e-233
Identity = 397/421 (94.30%), Postives = 407/421 (96.67%), Query Frame = 0

Query: 63  MLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSV 122
           MLLQFIL+LIATPLAKLDAFLLKLFNFISFNGG LGILSKILRGKGFVKV EDS+EYTSV
Sbjct: 1   MLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSV 60

Query: 123 VGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFW 182
           VGFADWRRDLDSSIK E+SFRYYSALT MATKISYESKPFVQSVVNDRWKMKLLGYFNFW
Sbjct: 61  VGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFW 120

Query: 183 NDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGF 242
           NDFQG+ TTQAF F+NTAIDPNV IVAFRGTSPLDAYDWQVD D SWYEIE VG IHSGF
Sbjct: 121 NDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGF 180

Query: 243 MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF 302
           MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF
Sbjct: 181 MKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF 240

Query: 303 VTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRV 362
           VTVL LHDESTILEKLDSVYTYGQPRVGDQQFAKFM+NC+QKYGFKYHRYVYSYDLVPRV
Sbjct: 241 VTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRV 300

Query: 363 PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPF 422
           PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPN+NYFSLLWVIPKYLSAWWELIRSFIIPF
Sbjct: 301 PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPF 360

Query: 423 VKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEIEDD 482
           VKGFDYYESLLMKGARLVGL+IPGL+AHFPLNYVNVTRLGKLNVPDEVEDP L YEIEDD
Sbjct: 361 VKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEIEDD 420

Query: 483 F 484
           F
Sbjct: 421 F 421

BLAST of CSPI04G24160 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 788.1 bits (2034), Expect = 4.3e-224
Identity = 378/484 (78.10%), Postives = 422/484 (87.19%), Query Frame = 0

Query: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
           MV  E EFSKNY+ILKP +A LLDLFLFLLPFGLRK KFIDCP G ED + NFTDRW I 
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
           +S+LLQ IL+  ATPLAKLD+FL+ LFNFISFNGG LG+L+KIL+GKG VK NEDS++YT
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
           SVVGFADWR DLDSSI    +FRYY  LT MA KI+YESKPFVQSVVNDRWKMKLLG+FN
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQGRTTTQAFMFKNTAI-DPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIH 240
           FWNDFQG+ TTQAFMF+NTA  DPN+ ++AFRGTSPLD YDWQVD DFSWY+IE VG IH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
           SGFMKALGLQKATGWPKEL K Q H+FAYYTLR++LRDIAKAN KARFI TGHSLGGALA
Sbjct: 241 SGFMKALGLQKATGWPKELTKPQ-HDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALA 300

Query: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360
           TLFVT+L LHDE+ ILEKL  VYTYGQPRVGD++FA+FM+N +Q+YGFKY+RYVYS DLV
Sbjct: 301 TLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLV 360

Query: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420
           PR+PSD V+FKYKHFGR +YFN+LYKGRIVK QPNKNYFSLLWVIPKYLSAWWEL+RSFI
Sbjct: 361 PRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFI 420

Query: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480
            P V GFDYYESLLM  AR++GL+IPGL AHFPLNYVN TRLGKL  PDEVEDPIL  +I
Sbjct: 421 TPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDDI 480

Query: 481 EDDF 484
           E D+
Sbjct: 481 EGDY 483

BLAST of CSPI04G24160 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 444.9 bits (1143), Expect = 8.3e-125
Identity = 220/469 (46.91%), Postives = 314/469 (66.95%), Query Frame = 0

Query: 7   EFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGK-EDYFTNFTDRWLIVVSMLL 66
           +F  +Y ++ P  A+ LDL L L    L   +FID P    + +  +F  RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVGF 126
           Q +L L++ P A +   L    N ++ NGGF  ++  ++ GK  VK ++ SA YTS +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 ADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWNDF 186
           +D R +LD  I    S  Y S L+ MA+KISYESKP++ SVV + WKM L+G ++F+N F
Sbjct: 127 SDRRIELDEKIN-VGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186

Query: 187 QGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKA 246
           Q    TQAF+FK ++ +P++ +V+FRGT P +A DW  D D SWYE+++VG +H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246

Query: 247 LGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTV 306
           LGLQK  GWPKE   +  H++AYYT+R+ LRD    N+  ++I TGHSLGGALA LF  +
Sbjct: 247 LGLQK-DGWPKE-NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAI 306

Query: 307 LCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVP-S 366
           L +H E  +L+KL+ +YT+GQPRVGD+ F +FM   ++K+G +Y R+VY+ D+VPRVP  
Sbjct: 307 LAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFD 366

Query: 367 DAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFVK 426
           D  LF YKH+G C  FNSLYKG++ ++ PN NYF+LLW+IP+ L+  WE IRSFI+ F K
Sbjct: 367 DKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFWK 426

Query: 427 GFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDP 474
           G +Y E+ LM+  R+VG+V PG + HFP +YVN TRLG L  P     P
Sbjct: 427 GDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTP 471

BLAST of CSPI04G24160 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 435.6 bits (1119), Expect = 5.0e-122
Identity = 220/461 (47.72%), Postives = 311/461 (67.46%), Query Frame = 0

Query: 9   SKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGK-EDYFTNFTDRWLIVVSMLLQF 68
           +KNY +L P  A + DL   L    L  +KFI     + ED  + F  RW+I VS+++Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 69  ILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVGFAD 128
           ++ L   PL  +   L    N +S NGGFL IL  + +G   +   + SA + S+ G  D
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKG-NIIWPEKASATFASLNGNLD 123

Query: 129 WRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWNDFQG 188
            R +L+  ++   S RY + L+ MA+K+SYE+  FV SV+++ WKM LLG+++ WN +Q 
Sbjct: 124 RRVELNPKVE-RGSKRYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQK 183

Query: 189 RTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKALG 248
           + +T+  + K+T+ DPN+ IV+FRGT P DA DW  D D SWYE+++VG IH GFMKALG
Sbjct: 184 QRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALG 243

Query: 249 LQKATGWPKE--LPKTQ--THEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFV 308
           LQK  GWPKE  L +TQ  T  +AYYT+R+ L++I   N  ++FI TGHSLGGALA LF 
Sbjct: 244 LQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFT 303

Query: 309 TVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVP 368
            VL +HDE  +LE+L+ VYT+GQPRVGD++F  FM + ++K+  KY RYVY  D+VPR+P
Sbjct: 304 AVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLP 363

Query: 369 SDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFV 428
            D     +KHFG C+Y++S YKG++ +E+PNKNYF+L+WV+PK ++A WELIRSF++P+ 
Sbjct: 364 FDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYW 423

Query: 429 KGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKL 465
           KG ++ E   ++  R+V L+IPGL AHFP  Y+NVT LG L
Sbjct: 424 KGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL 461

BLAST of CSPI04G24160 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 6.8e-111
Identity = 209/470 (44.47%), Postives = 300/470 (63.83%), Query Frame = 0

Query: 10  KNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPY-GKEDYFTNFTDRWLIVVSMLLQFI 69
           KNY +L P  A + DL   L    L  +KF+D      E+    F  RW+I VS+++Q +
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 70  LSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVGFADW 129
           L ++  PL+ L   L    N  S NGGF  I   +++G+ F+   + SA + S+ G  D 
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGR-FIWPEKTSATFASINGNLDQ 124

Query: 130 RRD--LDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWNDFQ 189
           + +  L  SIK     RY   L+ MA+K++YE++ F++SV+ D W+M LLG+++  NDF 
Sbjct: 125 KVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFD 184

Query: 190 GRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKAL 249
              +T+  + ++T  +PN+ +V+FRGT P +A DW  D D SW+ + +VG IH GFMKAL
Sbjct: 185 QTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKAL 244

Query: 250 GLQKATGWPKELPKTQTH----EFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLF 309
           GL K  GW +E+   QT     + AYYT+ +QL+++ + N  ++FI +GHSLGGALA LF
Sbjct: 245 GLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILF 304

Query: 310 VTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRV 369
             VL +HDE  +LE+L+ VYT+GQPRVGD+ F  +M + ++++  KY RYVY  D+VPR+
Sbjct: 305 TAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRL 364

Query: 370 PSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPF 429
           P D     +KHFG C+Y +S YKG++ +E+PNKNYF++ WVIPK ++A WELIRSFII  
Sbjct: 365 PFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISC 424

Query: 430 VKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVED 473
            +G +Y E  L+   RLV L+IPGL AHFP  YVNV  LG  N P +V D
Sbjct: 425 WQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLG--NFPPQVPD 469

BLAST of CSPI04G24160 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 1.0e-98
Identity = 187/465 (40.22%), Postives = 278/465 (59.78%), Query Frame = 0

Query: 5   ENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIVVSML 64
           +++  + Y+IL+PE     +L   L    + K + +D    +E    +F  RWLI VS++
Sbjct: 6   DDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEH---SFRHRWLIFVSLV 65

Query: 65  LQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVG 124
           L  +L   +  LA + + L    NF+S N  F G+    LRG+  V     S  Y S +G
Sbjct: 66  LLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGL---FLRGE-VVMPQRTSENYQSFIG 125

Query: 125 FADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWND 184
             D R  LD ++  E   +YY+AL+ MA+KI+YE+   ++ VV + W MK LG  ++WN+
Sbjct: 126 HLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNE 185

Query: 185 FQGRTTTQAFMFKNTAI------DPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLI 244
           +Q + TTQAF+                 +VAFRGT   ++ DW  DFD +W+E+ ++G I
Sbjct: 186 YQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNI 245

Query: 245 HSGFMKALGLQKATGWPKELPKTQTHE--FAYYTLRKQLRDIAKANEKARFIFTGHSLGG 304
           H GFMKALGLQ    WPKE       +   AYY++R  L+ +   N+  +F+ TGHSLGG
Sbjct: 246 HGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGG 305

Query: 305 ALATLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSY 364
           ALA LF  VL +H E+ +LE++  VYTYGQPRVGD +F +FM   ++KY  KY+R+VY+ 
Sbjct: 306 ALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNN 365

Query: 365 DLVPRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIR 424
           D+VPR+P D     +KHFG C+Y++  Y+ ++++EQ ++N+F L  +I    SA  E IR
Sbjct: 366 DIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIR 425

Query: 425 SFIIPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRL 462
           SF I   KG +Y E  L+KG R +G+++PG++ H P +YVN TRL
Sbjct: 426 SFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

BLAST of CSPI04G24160 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 355.5 bits (911), Expect = 6.6e-98
Identity = 176/384 (45.83%), Postives = 256/384 (66.67%), Query Frame = 0

Query: 7   EFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGK-EDYFTNFTDRWLIVVSMLL 66
           +F  +Y ++ P  A+ LDL L L    L   +FID P    + +  +F  RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVVGF 126
           Q +L L++ P A +   L    N ++ NGGF  ++  ++ GK  VK ++ SA YTS +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 ADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWNDF 186
           +D R +LD  I    S  Y S L+ MA+KISYESKP++ SVV + WKM L+G ++F+N F
Sbjct: 127 SDRRIELDEKIN-VGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186

Query: 187 QGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFMKA 246
           Q    TQAF+FK ++ +P++ +V+FRGT P +A DW  D D SWYE+++VG +H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246

Query: 247 LGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTV 306
           LGLQK  GWPKE   +  H++AYYT+R+ LRD    N+  ++I TGHSLGGALA LF  +
Sbjct: 247 LGLQK-DGWPKE-NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAI 306

Query: 307 LCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVP-S 366
           L +H E  +L+KL+ +YT+GQPRVGD+ F +FM   ++K+G +Y R+VY+ D+VPRVP  
Sbjct: 307 LAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFD 366

Query: 367 DAVLFKYKHFGRCVYFNSLYKGRI 389
           D  LF YKH+G C  FNSLYKG++
Sbjct: 367 DKYLFSYKHYGPCNSFNSLYKGKV 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1S3ZP856.3e-8537.92Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
F4JFU81.2e-8336.40Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
Q5VKJ75.9e-7534.39Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
O599522.5e-0931.14Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
P195152.8e-0831.76Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L2D56.2e-28599.79Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE... [more]
A0A1S3BUY04.3e-27094.41uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=... [more]
A0A5D3D9F32.5e-23394.30Lipase class 3 family protein-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1FNK82.1e-22478.10uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC1114458... [more]
A0A6J1J3Y93.7e-22176.86uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121... [more]
Match NameE-valueIdentityDescription
XP_011654113.11.3e-28499.79uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical ... [more]
XP_008452563.18.8e-27094.41PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo][more]
XP_038899591.14.0e-24685.30triacylglycerol lipase OBL1-like [Benincasa hispida][more]
KAA0064390.15.1e-23394.30lipase class 3 family protein-like 1 [Cucumis melo var. makuwa] >TYK20196.1 lipa... [more]
XP_022940183.14.3e-22478.10uncharacterized protein LOC111445886 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G45201.18.3e-12546.91triacylglycerol lipase-like 1 [more]
AT5G42930.15.0e-12247.72alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.16.8e-11144.47alpha/beta-Hydrolases superfamily protein [more]
AT5G67050.11.0e-9840.22alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.26.6e-9845.83triacylglycerol lipase-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 207..365
e-value: 3.6E-36
score: 124.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 133..406
e-value: 1.5E-46
score: 161.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 150..397
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..467
NoneNo IPR availableCDDcd00519Lipase_3coord: 142..389
e-value: 1.38318E-42
score: 149.164
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G24160.1CSPI04G24160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004806 triglyceride lipase activity